##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939892_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1883511 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33441270053639 31.0 31.0 34.0 30.0 34.0 2 31.56031528353166 31.0 31.0 34.0 30.0 34.0 3 31.58462626446036 31.0 31.0 34.0 30.0 34.0 4 35.41874881537724 37.0 35.0 37.0 33.0 37.0 5 35.14897975111374 37.0 35.0 37.0 32.0 37.0 6 35.18581946163309 37.0 35.0 37.0 32.0 37.0 7 35.087221683335 37.0 35.0 37.0 32.0 37.0 8 35.041605278652476 37.0 35.0 37.0 32.0 37.0 9 36.60881831855508 39.0 35.0 39.0 32.0 39.0 10-11 36.46728848411291 38.0 35.0 39.0 32.0 39.0 12-13 36.379356159852534 38.0 35.0 39.0 32.0 39.0 14-15 37.34407656764415 39.0 36.0 41.0 32.0 41.0 16-17 37.272996812867035 39.0 36.0 40.5 32.0 41.0 18-19 37.29499243699665 39.0 36.0 40.0 31.5 41.0 20-21 37.224971343411326 39.0 36.0 40.0 31.0 41.0 22-23 37.09478919953215 39.0 36.0 40.0 31.0 41.0 24-25 36.92029061683208 39.0 36.0 40.0 31.0 41.0 26-27 36.58710833119636 38.0 35.0 40.0 30.0 41.0 28-29 36.33204796786427 38.0 35.0 40.0 30.0 41.0 30-31 35.99428991919878 38.0 34.5 40.0 29.5 41.0 32-33 35.86591211837892 38.0 34.0 40.0 29.0 41.0 34-35 35.66261280130565 38.0 34.0 40.0 28.5 41.0 36-37 35.62160587328664 38.0 34.0 40.0 28.0 41.0 38-39 35.46242602246549 38.0 34.0 40.0 27.0 41.0 40-41 35.310519290835046 38.0 34.0 40.0 27.0 41.0 42-43 34.99849615956583 38.0 33.5 40.0 26.0 41.0 44-45 34.650046907079386 38.0 33.0 40.0 25.0 41.0 46-47 34.234503276062625 38.0 33.0 40.0 24.0 41.0 48-49 33.80603351931579 37.0 33.0 40.0 23.0 41.0 50-51 33.49871251083748 37.0 32.5 40.0 22.0 41.0 52-53 33.18719986238466 37.0 32.0 40.0 20.5 41.0 54-55 32.86285718533101 36.0 31.5 39.5 19.5 41.0 56-57 32.43799664562617 36.0 31.0 39.0 17.5 40.5 58-59 32.02418488662928 35.5 31.0 39.0 15.0 40.0 60-61 31.662527853567088 35.0 30.0 39.0 13.0 40.0 62-63 31.32872969682683 35.0 30.0 38.0 10.0 40.0 64-65 30.85335710808166 35.0 29.5 38.0 8.0 40.0 66-67 29.905764553538578 34.0 28.0 37.0 4.5 39.5 68-69 27.974704156227386 33.0 24.0 36.0 2.0 39.0 70-71 27.31253361408561 32.0 22.5 35.5 2.0 39.0 72-73 27.866273411729477 33.0 25.5 35.0 2.0 38.0 74-75 27.98376754900821 33.0 26.0 35.0 2.0 37.5 76-77 27.585269212656577 32.0 26.0 34.5 2.0 36.5 78-79 28.111653714791153 33.0 26.5 35.0 2.0 37.0 80-81 27.94413199604356 33.0 26.5 35.0 2.0 36.0 82-83 27.509957202267465 33.0 26.0 35.0 2.0 36.0 84-85 27.164222030027965 33.0 25.5 35.0 2.0 35.5 86-87 26.78919767391855 32.5 25.0 35.0 2.0 35.0 88-89 26.328978699885482 32.0 24.5 34.5 2.0 35.0 90-91 25.832595880777973 32.0 23.0 34.0 2.0 35.0 92-93 25.336269870470627 32.0 21.0 34.0 2.0 35.0 94-95 24.501247935371758 31.0 18.0 34.0 2.0 35.0 96-97 23.971603032846637 31.0 11.0 34.0 2.0 35.0 98-99 23.288757538448145 31.0 2.0 34.0 2.0 35.0 100-101 21.83646525026931 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30435677031913855 1101 2 0.38738910148103756 1101 3 0.36157675078554874 1101 4 0.33567055841417215 1101 5 0.3379728498244816 1101 6 0.18756873264413088 1101 7 0.24431212161743332 1101 8 0.24264851073080962 1101 9 0.5570574994258735 1101 10-11 0.5032328990524562 1101 12-13 0.38505271779779093 1101 14-15 0.5032502300506181 1101 16-17 0.5490543555818874 1101 18-19 0.3837593006762674 1101 20-21 0.4248056433796208 1101 22-23 0.3174942622900332 1101 24-25 0.3014592714267792 1101 26-27 0.42310598553446965 1101 28-29 0.6009481497106393 1101 30-31 0.4563249531577114 1101 32-33 0.4517237256105986 1101 34-35 0.5211311034437571 1101 36-37 0.6870662198150939 1101 38-39 0.8019802209979545 1101 40-41 0.748233225820087 1101 42-43 0.44610131942094 1101 44-45 0.5372572771868747 1101 46-47 0.5732037533920149 1101 48-49 0.5971406394437935 1101 50-51 0.47338006548846323 1101 52-53 0.491103031467496 1101 54-55 0.25344641426128334 1101 56-57 0.3118308289115461 1101 58-59 0.4736059467635343 1101 60-61 0.2808608926705354 1101 62-63 0.40518688218034526 1101 64-65 0.07097479490791514 1101 66-67 0.08915839224082944 1101 68-69 -7.8902837736650575 1101 70-71 -5.881482724256614 1101 72-73 -1.182681142348848 1101 74-75 -0.09043195952628125 1101 76-77 0.11453091079511424 1101 78-79 0.03621373175800002 1101 80-81 -0.03018361545142767 1101 82-83 6.15371726226499E-4 1101 84-85 0.03463856319870828 1101 86-87 -0.004045103851780141 1101 88-89 0.02884445166423788 1101 90-91 -0.2293788085133599 1101 92-93 -0.30503585592999016 1101 94-95 0.08625469237271588 1101 96-97 -0.30298285075625486 1101 98-99 -0.08773411134295372 1101 100-101 0.016436224752830242 1102 1 0.24775229755612926 1102 2 0.18525485361508487 1102 3 0.2446012466149199 1102 4 0.14374358295061285 1102 5 0.3063048227296292 1102 6 0.2040740768933773 1102 7 0.25753467142534703 1102 8 0.2500389861090895 1102 9 0.4072159791575203 1102 10-11 0.4026690827874191 1102 12-13 0.31735974004383394 1102 14-15 0.4446936951221261 1102 16-17 0.42711811093222707 1102 18-19 0.4384406457539498 1102 20-21 0.3475188549647825 1102 22-23 0.39100611628968807 1102 24-25 0.33386208203081225 1102 26-27 0.3340624071616247 1102 28-29 0.320965936801592 1102 30-31 0.2902598191047687 1102 32-33 0.23465065640338878 1102 34-35 0.1699842827847462 1102 36-37 0.15258824859932218 1102 38-39 0.37665427661713835 1102 40-41 0.3103388031695644 1102 42-43 0.3569602775359968 1102 44-45 0.35827645110238393 1102 46-47 0.39690139721160733 1102 48-49 0.28401194902851756 1102 50-51 0.37602848414298506 1102 52-53 0.2139198487965075 1102 54-55 0.1807274665423435 1102 56-57 -0.039373353085316865 1102 58-59 0.028290337575974434 1102 60-61 0.12628807727454827 1102 62-63 0.2865126194512051 1102 64-65 0.11075742081859019 1102 66-67 0.47300422120861185 1102 68-69 -4.16090974322395 1102 70-71 0.6757336873805322 1102 72-73 0.7976733608715065 1102 74-75 0.618239704175874 1102 76-77 0.48060741559113396 1102 78-79 0.20094356493295962 1102 80-81 0.13610182674865 1102 82-83 0.22314335187616052 1102 84-85 0.1322589306361479 1102 86-87 0.05364263523839696 1102 88-89 -0.12576440492632557 1102 90-91 -0.05132642390609732 1102 92-93 0.0031815871959857134 1102 94-95 0.21171561356534596 1102 96-97 -0.23641070339142445 1102 98-99 -0.07045388141028397 1102 100-101 0.2626686508633078 1103 1 0.1968675744321544 1103 2 0.15254107657776572 1103 3 0.14675290060258206 1103 4 0.15796152711398292 1103 5 0.2035658203898265 1103 6 0.16347956214612225 1103 7 0.1767912447042903 1103 8 0.14877001353739416 1103 9 0.2635637004072109 1103 10-11 0.36211986843665755 1103 12-13 0.23228559541740879 1103 14-15 0.3622682838200575 1103 16-17 0.2689973959669203 1103 18-19 0.28447046796201647 1103 20-21 0.28823365183525596 1103 22-23 0.1941507549686179 1103 24-25 0.20821549862499467 1103 26-27 0.1470753862994485 1103 28-29 0.029220153949495398 1103 30-31 0.10829591037728648 1103 32-33 0.18117245367811563 1103 34-35 -0.03473982121169428 1103 36-37 0.1344710249547063 1103 38-39 0.4568764297615715 1103 40-41 0.42807134350351106 1103 42-43 0.32608735548432577 1103 44-45 0.3613986054718481 1103 46-47 0.4171250254900798 1103 48-49 0.2747544620852338 1103 50-51 0.11777296540622473 1103 52-53 0.19926089570270733 1103 54-55 0.24855351710319695 1103 56-57 0.2632095606770193 1103 58-59 0.17452933276978655 1103 60-61 0.018825966270966887 1103 62-63 -0.1614964345029719 1103 64-65 -0.052437704195192225 1103 66-67 0.34093838020866585 1103 68-69 -3.9921984335907617 1103 70-71 0.39728822854748813 1103 72-73 0.7851921445852099 1103 74-75 0.6192147473579972 1103 76-77 0.2998179993971206 1103 78-79 0.24122557074774065 1103 80-81 0.22409663468517493 1103 82-83 -0.030542696724658924 1103 84-85 -0.15799615015423996 1103 86-87 -0.18038533820306668 1103 88-89 -0.10511827069627344 1103 90-91 -0.2680900972246434 1103 92-93 -0.18139430411164525 1103 94-95 -0.26888050392269847 1103 96-97 -0.3962680186270582 1103 98-99 -0.36644297154352756 1103 100-101 -0.3320150477315913 1104 1 0.08706562055529687 1104 2 0.1612894811411003 1104 3 0.11916325564116903 1104 4 0.08843864435008442 1104 5 0.1538188707350514 1104 6 0.23056496183698272 1104 7 0.0482395745021833 1104 8 0.2702708387503563 1104 9 0.24980616089349894 1104 10-11 0.18446303931933272 1104 12-13 0.11812178378642102 1104 14-15 0.19235109938981765 1104 16-17 0.1278943338756875 1104 18-19 0.09652940048707848 1104 20-21 0.10294403924393691 1104 22-23 0.006508574690634816 1104 24-25 0.157434725962311 1104 26-27 0.24315758239838203 1104 28-29 0.1900167276074214 1104 30-31 0.07745928059789264 1104 32-33 0.21506267067745455 1104 34-35 0.24907495700826843 1104 36-37 0.35658885209840463 1104 38-39 0.3031411752373643 1104 40-41 0.12504137298572715 1104 42-43 0.26041808507832087 1104 44-45 0.28500555671270433 1104 46-47 0.1320208052577243 1104 48-49 0.22474516004731981 1104 50-51 0.19833559655124589 1104 52-53 -0.06357367216650545 1104 54-55 -0.0771755457052663 1104 56-57 -0.03223076986702722 1104 58-59 -0.05943375434965503 1104 60-61 -0.13558586811585016 1104 62-63 0.12639739956762597 1104 64-65 0.21113329579133833 1104 66-67 0.37921763786114937 1104 68-69 -3.730270003919408 1104 70-71 0.7726702230939502 1104 72-73 0.9090436671018765 1104 74-75 0.6089957230195786 1104 76-77 0.4814001388857925 1104 78-79 0.06360639867400764 1104 80-81 -0.08316563839373003 1104 82-83 -0.02297275811810806 1104 84-85 -0.024044809039779125 1104 86-87 0.017446314362576487 1104 88-89 -0.1293826241397973 1104 90-91 -0.15219680481695264 1104 92-93 -0.32923211647183237 1104 94-95 0.04700174075631125 1104 96-97 -0.37074626906585095 1104 98-99 -0.3160489940769011 1104 100-101 -0.2840197740646353 1105 1 0.13559233435629992 1105 2 0.09786357217485175 1105 3 0.1707790141253973 1105 4 0.10188440438873414 1105 5 0.1734852935123996 1105 6 0.20877086052622218 1105 7 0.13200070422126942 1105 8 0.2051994194432325 1105 9 0.24220136851789675 1105 10-11 0.1713115800922509 1105 12-13 0.10113797849920303 1105 14-15 0.13366717103585302 1105 16-17 0.19125836294043097 1105 18-19 0.09579302965914138 1105 20-21 0.19869576123783617 1105 22-23 0.20029167424995364 1105 24-25 0.13827126302441428 1105 26-27 0.12252172433205999 1105 28-29 0.2708358392892549 1105 30-31 0.14259356988494432 1105 32-33 0.06552642341130621 1105 34-35 0.1200142741115755 1105 36-37 -0.055054321657387106 1105 38-39 0.2568386726161336 1105 40-41 0.1698669261177983 1105 42-43 -0.0669372372367647 1105 44-45 0.040408662192696454 1105 46-47 0.05591520943347916 1105 48-49 0.04280290340117432 1105 50-51 0.16764069020771188 1105 52-53 0.18117965474451836 1105 54-55 0.2651824349703915 1105 56-57 0.2004250362993787 1105 58-59 0.034416522030731755 1105 60-61 0.2772510926998173 1105 62-63 0.24043413741762976 1105 64-65 0.1329188329237354 1105 66-67 0.7473802800240357 1105 68-69 1.7554901931457998 1105 70-71 1.951512296674597 1105 72-73 1.1877465356560961 1105 74-75 0.6484592589262554 1105 76-77 0.4416121047756114 1105 78-79 0.32030625936997126 1105 80-81 0.4141158537732679 1105 82-83 0.3542201418314974 1105 84-85 0.2871828783053374 1105 86-87 0.33930546382580573 1105 88-89 0.43061093044966015 1105 90-91 0.429385657329874 1105 92-93 0.40147274015986056 1105 94-95 0.6056871542279119 1105 96-97 0.2646516583039791 1105 98-99 0.46264372617346794 1105 100-101 0.5620297140153134 1106 1 0.3605211706497613 1106 2 0.2746462236875473 1106 3 0.30504138418092097 1106 4 0.1854145810404546 1106 5 0.2662166890965594 1106 6 0.33561966945372745 1106 7 0.26483528518801336 1106 8 0.291710796340773 1106 9 0.35289857896433574 1106 10-11 0.17894823326539466 1106 12-13 0.34151527495294687 1106 14-15 0.4461047915785912 1106 16-17 0.3448515259128868 1106 18-19 0.4062076834070112 1106 20-21 0.3217226581709056 1106 22-23 0.476131575961972 1106 24-25 0.3817593655787661 1106 26-27 0.27719542300163624 1106 28-29 0.5006766843619204 1106 30-31 0.6055849898711259 1106 32-33 0.5896397520455423 1106 34-35 0.7811264357619478 1106 36-37 0.7492966106283347 1106 38-39 0.9830861422477213 1106 40-41 0.844044314039273 1106 42-43 0.5943322839811529 1106 44-45 0.7653859678888182 1106 46-47 0.7540245309096534 1106 48-49 0.9292780012828032 1106 50-51 0.9993055252178706 1106 52-53 0.9426595995664186 1106 54-55 0.9850917002486099 1106 56-57 0.9890421365171065 1106 58-59 1.0979730554140659 1106 60-61 1.1460256959013968 1106 62-63 1.050132373556309 1106 64-65 1.0115092205249177 1106 66-67 1.6359566925021056 1106 68-69 2.920075552677222 1106 70-71 3.224470725596678 1106 72-73 2.1557924511860094 1106 74-75 1.4653787720969191 1106 76-77 1.0622387703303566 1106 78-79 0.9912348693843605 1106 80-81 1.122840903982258 1106 82-83 1.11157753156958 1106 84-85 0.9755033723373572 1106 86-87 1.1305906386688847 1106 88-89 1.3517740526971984 1106 90-91 1.2329027830969501 1106 92-93 1.1534710074783092 1106 94-95 1.3993620698114775 1106 96-97 1.0778305287252117 1106 98-99 1.2196389897168807 1106 100-101 1.311379294469475 1107 1 0.10783005544379876 1107 2 0.10853187308252643 1107 3 0.10541089828942063 1107 4 0.08579810670685362 1107 5 0.1456284969296746 1107 6 0.10738795521564981 1107 7 0.09801601201856869 1107 8 0.13471210462926564 1107 9 0.14422059117611497 1107 10-11 0.13847329576073975 1107 12-13 0.028187106345022528 1107 14-15 0.2569893553493756 1107 16-17 0.16010818452562603 1107 18-19 0.07647255072895831 1107 20-21 0.13110749647047015 1107 22-23 0.02141063134401122 1107 24-25 0.16030302705951982 1107 26-27 0.2720969537914897 1107 28-29 0.13181101772946846 1107 30-31 0.22668761801578086 1107 32-33 0.19847086226599941 1107 34-35 0.1788291648115461 1107 36-37 0.061907229977208544 1107 38-39 0.37590451281215564 1107 40-41 0.24646358872934826 1107 42-43 0.20020603224124756 1107 44-45 0.17348725515996932 1107 46-47 0.24843728642371588 1107 48-49 0.3851197347623341 1107 50-51 0.2930191420082764 1107 52-53 0.3112166589205785 1107 54-55 0.26265916888689134 1107 56-57 0.3012804720309177 1107 58-59 0.295032990667643 1107 60-61 0.3585431492518758 1107 62-63 0.20383115330168167 1107 64-65 0.09003830020995096 1107 66-67 0.5621802735195942 1107 68-69 2.0145450875858266 1107 70-71 2.2477832696882807 1107 72-73 1.2659533016984383 1107 74-75 0.727275562309682 1107 76-77 0.47194744940431477 1107 78-79 0.5913125574990303 1107 80-81 0.5362550427303496 1107 82-83 0.7344014633104834 1107 84-85 0.6709591742899619 1107 86-87 0.4915653000155018 1107 88-89 0.5936071609223603 1107 90-91 0.6269845019783915 1107 92-93 0.7160842064744735 1107 94-95 1.0955232016412282 1107 96-97 0.6038732900448416 1107 98-99 1.02919788632299 1107 100-101 0.8981358197551792 1108 1 -0.14289416995505633 1108 2 -0.18395325125666417 1108 3 -0.08563288712176487 1108 4 -0.08508826932140323 1108 5 -0.08897074921213743 1108 6 -0.1268509561941613 1108 7 -0.17081977490981615 1108 8 -0.1770888578437706 1108 9 -0.23773659536822578 1108 10-11 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-0.06574726921659035 2316 76-77 -0.36845485344064954 2316 78-79 -0.7126904890212167 2316 80-81 -0.5915566174494273 2316 82-83 -0.5092060568452013 2316 84-85 -0.5297283199181742 2316 86-87 -0.559758140814818 2316 88-89 -0.7520278010830133 2316 90-91 -0.6061582790428268 2316 92-93 -0.38529309868723516 2316 94-95 -0.07319523270220785 2316 96-97 0.1339052611318543 2316 98-99 0.06339913041528789 2316 100-101 -0.2976902737486675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 54.0 5 117.0 6 300.0 7 791.0 8 1671.0 9 3006.0 10 4725.0 11 7277.0 12 9794.0 13 12191.0 14 15021.0 15 19147.0 16 23285.0 17 26857.0 18 23778.0 19 19379.0 20 18232.0 21 18472.0 22 19458.0 23 21954.0 24 25715.0 25 30750.0 26 37366.0 27 45774.0 28 55495.0 29 65843.0 30 77682.0 31 91962.0 32 109109.0 33 131923.0 34 163618.0 35 203716.0 36 243808.0 37 236329.0 38 111362.0 39 7530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35218706670356 18.998371296584928 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25.602319285631992 29.653450391317065 22.280968892669062 20-21 23.25263829093645 24.66210709679954 29.952678800389272 22.132575811874737 22-23 22.880142457357564 24.7697252630858 30.031361643229054 22.31877063632758 24-25 22.590713023063074 25.256296958574914 29.903289525903386 22.249700492458622 26-27 22.050565302454732 25.531681625480157 29.671994499616407 22.745758572448707 28-29 22.203896502778456 25.885667154851088 29.574419454746575 22.336016887623874 30-31 22.30448326986558 25.563650698631935 29.337444468483582 22.794421563018897 32-33 21.942169021405423 25.597042482458587 29.290992722746267 23.169795773389723 34-35 22.8190793342255 25.672330356761243 29.172698435116136 22.335891873897125 36-37 23.05835420695901 25.146620279650776 29.0854774307078 22.70954808268241 38-39 22.52184770365615 25.36145796437779 29.06700493883387 23.049689393132187 40-41 22.65910586294462 25.463315805194238 29.27749308281594 22.6000852490452 42-43 22.63523290314094 25.519253689498793 28.790567213519903 23.05494619384036 44-45 22.416223125101308 25.708652315672275 29.07707924225474 22.798045316971677 46-47 22.98386871781137 26.027558503830285 28.15294148594003 22.835631292418316 48-49 22.728702718616447 26.23705604203711 27.725312286437315 23.30892895290913 50-51 22.776009210425975 26.52222190632754 27.68946750165601 23.012301381590465 52-53 23.182280779073185 26.038680568782823 27.747728075117973 23.03131057702602 54-55 22.456638792917282 26.38798761021092 27.927600324380634 23.227773272491156 56-57 22.618081527690883 26.41718014928084 27.599163840343643 23.365574482684632 58-59 22.495061155125526 25.969549475928886 27.637353782713127 23.898035586232456 60-61 22.080621743005352 26.314167224847417 28.071460941550708 23.53375009059652 62-63 22.260387432186626 26.32210198367064 27.976700550789552 23.44081003335319 64-65 21.883571499111724 26.306637599008614 27.878298119062006 23.93149278281766 66-67 21.720916108803078 26.460788943558455 27.994754274117895 23.823540673520572 68-69 22.281091154862885 25.915474557449862 28.247009562202308 23.55642472548494 70-71 21.86344033081529 26.100751734191952 27.839115394837048 24.19669254015571 72-73 21.925851255671937 26.422465383244585 27.50934905073223 24.14233431035125 74-75 22.401271373776723 26.703124398400956 26.946406072217112 23.94919815560521 76-77 22.221888812617756 26.36464182293029 27.16194810936838 24.251521255083574 78-79 22.389966759927887 26.52391036622484 27.063965303534342 24.02215757031293 80-81 22.609737663862195 26.59289544011738 26.72390290210066 24.073463993919766 82-83 22.472558554889925 26.65758964444318 26.671951125305647 24.197900675361247 84-85 22.34786583714804 26.73318106378438 26.589087773732125 24.329865325335465 86-87 22.63730505163449 26.578627481743233 26.286030721332516 24.49803674528977 88-89 22.258245458669773 26.64893909312232 26.238985636630403 24.8538298115775 90-91 22.170675079386907 26.679230176634206 26.29114885768334 24.858945886295544 92-93 22.37557437371397 26.31297271597102 26.44015854824978 24.87129436206523 94-95 21.855249831823016 25.91206123873905 27.00167805027349 25.23101087916444 96-97 22.359784889995737 25.398246887980573 26.882378476255457 25.359589745768226 98-99 22.09640565651502 25.875911807983076 26.459861863446914 25.567820672054985 100-101 21.903399580159775 25.602289130109853 26.787526704615296 25.706784585115077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1164.0 1 798.5 2 502.0 3 610.0 4 589.5 5 598.5 6 947.0 7 1899.5 8 3068.0 9 3602.0 10 3211.5 11 2672.0 12 2538.5 13 2570.5 14 2818.5 15 3057.5 16 3224.0 17 3501.5 18 3826.0 19 4205.0 20 4664.5 21 5316.5 22 6120.5 23 7064.5 24 8354.5 25 10005.5 26 11870.5 27 13969.0 28 15922.5 29 17832.5 30 20198.5 31 24091.0 32 29507.0 33 30509.5 34 28800.5 35 29501.0 36 31830.0 37 34477.5 38 37195.5 39 39173.5 40 41022.0 41 44512.5 42 47032.0 43 49466.5 44 52662.5 45 57306.5 46 61555.0 47 64672.5 48 67346.5 49 67876.0 50 68302.0 51 68804.5 52 69873.0 53 73632.5 54 76530.0 55 74003.0 56 68605.0 57 63604.5 58 59309.5 59 52881.5 60 44332.0 61 37072.5 62 31215.5 63 23847.5 64 16856.5 65 12630.5 66 9277.0 67 7041.5 68 5253.0 69 3677.0 70 2738.5 71 2063.5 72 1698.5 73 1608.0 74 1306.5 75 939.5 76 650.5 77 389.0 78 250.5 79 158.5 80 93.0 81 64.5 82 48.5 83 29.0 84 15.5 85 12.5 86 7.5 87 4.0 88 2.0 89 1.0 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022192596698399954 2 0.02155548865921144 3 1.0618467319808593E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.716463561933007E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.327308414976074E-4 26-27 3.185540195942577E-4 28-29 7.963850489856443E-5 30-31 4.5128486109186517E-4 32-33 0.0020971472956621967 34-35 0.004273933096222958 36-37 0.10353005636813375 38-39 0.09511492101718547 40-41 0.10432644141711941 42-43 0.10557411132719693 44-45 0.0996012234598046 46-47 0.09198247315784193 48-49 0.09264612736532996 50-51 0.09187628848464384 52-53 0.07175429291360655 54-55 0.0612951026035951 56-57 0.0327579716816095 58-59 0.01202541423968323 60-61 0.008521320024146396 62-63 0.0017520471077684177 64-65 0.004725217957314823 66-67 0.16580736719881115 68-69 0.43315382814329195 70-71 0.4131380172454528 72-73 0.1933091975571154 74-75 0.026997453160613343 76-77 0.032306686820517634 78-79 0.00485794879881243 80-81 0.00161931626627081 82-83 2.654616829952148E-5 84-85 4.778310293913866E-4 86-87 0.0016724086028698532 88-89 2.389155146956933E-4 90-91 7.432927123866014E-4 92-93 0.0024687936518554975 94-95 0.08308950677750222 96-97 0.015317139108823893 98-99 0.016033885652910973 100-101 0.00931770507313204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1883511.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.26303927398469 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14228588497652 32.20116080052738 2 13.962057351738515 12.639303005147342 3 5.6473869330682644 7.668536896405705 4 2.8654997470151837 5.188049103549659 5 1.650790558874696 3.7359898949734247 6 1.0633553025557918 2.8878415693069606 7 0.6995754045569579 2.2165436308102717 8 0.4950271759506231 1.7925147605394227 9 0.38589549944623414 1.572012283237995 >10 1.905246835199323 15.258972717867813 >50 0.11184865423768378 3.464550331999878 >100 0.06199124588431023 5.389017666702286 >500 0.004695795582280225 1.5321376958449844 >1k 0.0039914262449381906 3.03361563409468 >5k 2.347897791140112E-4 0.8245721288798804 >10k+ 1.173948895570056E-4 0.595181880112356 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11201 0.5946872622458801 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9106 0.48345881707088517 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6412 0.34042806227306344 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2821 0.1497734815459002 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2676 0.14207509273903896 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2568 0.13634112038634233 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2541 0.13490762729816816 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2530 0.13432361159557868 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2372 0.1259350224129299 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2019 0.10719342759346773 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1967 0.10443262609031749 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.1236934639617182E-4 2 0.0 0.0 0.0 0.0 2.1236934639617182E-4 3 0.0 0.0 0.0 0.0 2.1236934639617182E-4 4 0.0 0.0 0.0 0.0 2.1236934639617182E-4 5 0.0 0.0 0.0 0.0 2.1236934639617182E-4 6 0.0 0.0 0.0 0.0 3.716463561933007E-4 7 0.0 0.0 0.0 0.0 3.716463561933007E-4 8 0.0 0.0 0.0 0.0 4.7783102939138664E-4 9 0.0 0.0 0.0 0.0 5.309233659904296E-4 10-11 0.0 0.0 0.0 2.6546168299521478E-5 6.371080391885154E-4 12-13 0.0 0.0 0.0 5.3092336599042956E-5 0.0014865854247732028 14-15 0.0 0.0 0.0 5.3092336599042956E-5 0.001884777949266025 16-17 0.0 0.0 0.0 5.3092336599042956E-5 0.0024687936518554975 18-19 0.0 0.0 0.0 5.3092336599042956E-5 0.0026811629982516696 20-21 0.0 0.0 0.0 7.963850489856443E-5 0.003212086364242099 22-23 0.0 0.0 0.0 4.2473869279234365E-4 0.003318271037440185 24-25 0.0 0.0 0.0 0.0013007622466765525 0.003397909542338749 26-27 0.0 0.0 0.0 0.0035306403838363566 0.003822648235131093 28-29 0.0 0.0 0.0 0.01146794470539328 0.0039288329083291785 30-31 0.0 0.0 0.0 0.0367133507582382 0.003981925244928222 32-33 0.0 0.0 0.0 0.0812047288282362 0.00414120225472535 34-35 0.0 0.0 0.0 0.14398641685660452 0.004273933096222958 36-37 0.0 0.0 0.0 0.24918888182760812 0.00430047926452248 38-39 0.0 0.0 0.0 0.41496970285811974 0.004565940947517694 40-41 0.0 0.0 0.0 0.600129226747282 0.00467212562071578 42-43 0.0 0.0 0.0 0.7813333715598156 0.004725217957314823 44-45 0.0 0.0 0.0 0.9961980577761426 0.004884494967111953 46-47 0.0 0.0 0.0 1.24764336390921 0.0050968643135081235 48-49 0.0 0.0 0.0 1.5192372117816142 0.005441964501401903 50-51 0.0 0.0 0.0 1.8311546893009916 0.00557469534289951 52-53 0.0 0.0 0.0 2.114959774591176 0.006185257213788505 54-55 0.0 0.0 0.0 2.3973313667931855 0.006477265065083241 56-57 0.0 0.0 0.0 2.7169472331194244 0.006609995906580848 58-59 0.0 0.0 0.0 3.050924576495704 0.006742726748078455 60-61 0.0 0.0 0.0 3.4021569292666727 0.0068489114212765415 62-63 0.0 0.0 0.0 3.737143027038334 0.007220557777469842 64-65 0.0 0.0 0.0 4.0665544294670966 0.0073001962823684066 66-67 0.0 0.0 0.0 4.431139504892724 0.007379834787266971 68-69 0.0 0.0 0.0 4.833791785659866 0.007432927123866014 70-71 0.0 0.0 0.0 5.294686359676158 0.007592204133663143 72-73 0.0 0.0 0.0 5.784383526297431 0.008149673667953094 74-75 0.0 0.0 0.0 6.2534808663182755 0.008521320024146396 76-77 0.0 0.0 0.0 6.747717427718765 0.00878678170714161 78-79 0.0 0.0 0.0 7.313655189696265 0.009078789558436346 80-81 0.0 0.0 0.0 7.904360526697216 0.009264612736532996 82-83 0.0 0.0 0.0 8.515718782635195 0.00990172077572151 84-85 0.0 0.0 0.0 9.100690147283451 0.009981359280620076 86-87 0.0 0.0 0.0 9.672468066286843 0.010167182458716726 88-89 0.0 0.0 0.0 10.269916130035874 0.010459190310011463 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4770 0.0 61.971188 1 GTATCAA 8935 0.0 43.70443 1 AGGTAAG 1165 0.0 32.57816 2 TATCAAC 12160 0.0 32.070133 2 ATCAACG 12410 0.0 31.38085 3 TCAACGC 12645 0.0 30.797655 4 TAAGGTG 1380 0.0 30.591743 5 CAACGCA 12890 0.0 30.212286 5 GTGGTAT 1890 0.0 29.623772 1 AACGCAG 13415 0.0 29.029917 6 TGGTATC 1905 0.0 28.141418 2 GGTAAGG 1420 0.0 28.05978 3 AAGGTAA 1320 0.0 27.678144 1 TAAGGTA 640 0.0 27.423008 4 GTAAGGT 1470 0.0 26.782684 4 ACGCAGA 15195 0.0 25.56681 7 CGCAGAG 15440 0.0 25.130396 8 GCAGAGT 16385 0.0 23.27571 9 GTACATG 10445 0.0 22.895084 1 AAGGTGT 1940 0.0 22.739168 6 >>END_MODULE