FastQCFastQC Report
Fri 27 May 2016
SRR939891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780173
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149900.5391750801119211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105940.3810554235293991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73450.264192192356375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77650.051.3688131
GTATCAA145350.042.7465671
TATCAAC185900.034.2904132
ATCAACG191900.033.0717663
TCAACGC193700.032.8379024
CAACGCA197700.032.0049865
AACGCAG202450.031.3712086
ACGCAGA227000.027.64417
CGCAGAG229100.027.3709878
GCAGAGT233200.025.1811899
GTACATG157450.024.9420781
TACATGG162200.024.5045052
GAGTACT120850.023.76446712-13
ACATGGG166300.022.8178123
AGTACTT124900.022.61413412-13
GTACTTT131950.021.8401814-15
AGAGTAC219050.020.87195210-11
CAGAGTA231050.020.81427610-11
CATGGGG55450.020.3587784
CATGGGA109150.020.3375384
Produced by FastQC (version 0.11.5)