FastQCFastQC Report
Fri 27 May 2016
SRR939891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780173
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165810.5964017347121924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128880.4635682743483948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92090.331238379769892No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28820.10366261380137134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81150.049.6506921
GTATCAA147850.042.185781
TATCAAC190350.032.6594662
ATCAACG190300.032.542363
TCAACGC191250.032.40424
CAACGCA194850.031.8055115
AACGCAG200400.030.998176
GTACATG163850.027.1820071
ACGCAGA229600.026.9121977
CGCAGAG229050.026.8533558
TACATGG165300.026.5087592
ACATGGG165950.025.2666453
GCAGAGT243200.024.6707979
AGTACTT132450.023.1482212-13
GAGTACT129900.022.98580412-13
CATGGGA110950.022.147154
GTACTTT138500.021.30348814-15
AGAGTAC228350.021.0325310-11
CATGGGG56500.020.6184464
CAGAGTA238900.020.38978610-11
Produced by FastQC (version 0.11.5)