##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939891_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2780173 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.455006217239 31.0 31.0 34.0 30.0 34.0 2 31.66790196149664 31.0 31.0 34.0 30.0 34.0 3 31.716112630401057 31.0 31.0 34.0 30.0 34.0 4 35.52460908008243 37.0 35.0 37.0 33.0 37.0 5 35.26239266405364 37.0 35.0 37.0 33.0 37.0 6 35.29879435560305 37.0 35.0 37.0 32.0 37.0 7 35.21953669789614 37.0 35.0 37.0 32.0 37.0 8 35.16147915975013 37.0 35.0 37.0 32.0 37.0 9 36.759707399503554 39.0 35.0 39.0 32.0 39.0 10-11 36.61580178643559 39.0 35.0 39.0 32.0 39.0 12-13 36.551800013884026 38.0 35.0 39.0 32.0 39.0 14-15 37.5628128177635 39.0 36.0 41.0 32.0 41.0 16-17 37.51019810637683 39.0 36.0 41.0 32.0 41.0 18-19 37.53013607426588 39.0 36.0 41.0 32.0 41.0 20-21 37.48219769057537 39.0 36.0 41.0 32.0 41.0 22-23 37.340440145271536 39.0 36.0 41.0 31.5 41.0 24-25 37.17604012412177 39.0 36.0 40.5 31.0 41.0 26-27 36.89727707592297 39.0 36.0 40.0 31.0 41.0 28-29 36.66481276524878 38.5 35.0 40.0 30.0 41.0 30-31 36.38522836528519 38.0 35.0 40.0 30.0 41.0 32-33 36.30776897696654 38.0 35.0 40.0 30.0 41.0 34-35 36.34817167852504 38.0 35.0 40.0 30.0 41.0 36-37 36.292385581760556 38.0 35.0 40.0 30.0 41.0 38-39 36.15981703296881 38.0 35.0 40.0 30.0 41.0 40-41 35.970659919364735 38.0 35.0 40.0 29.5 41.0 42-43 35.726510904177545 38.0 35.0 40.0 28.5 41.0 44-45 35.42034632377194 38.0 34.0 40.0 27.0 41.0 46-47 35.04133447810622 38.0 34.0 40.0 26.0 41.0 48-49 34.6880636564703 38.0 33.5 40.0 24.5 41.0 50-51 34.41003383602387 38.0 33.0 40.0 24.0 41.0 52-53 34.12667808801827 38.0 33.0 40.0 23.5 41.0 54-55 33.81471710573406 38.0 33.0 40.0 23.0 41.0 56-57 32.45069533442703 36.5 31.5 39.5 15.0 41.0 58-59 29.057467826642444 34.5 23.0 39.0 7.0 40.0 60-61 29.039406000993463 34.0 23.5 38.0 7.0 40.0 62-63 31.02262467119852 35.0 29.0 38.5 6.5 40.0 64-65 30.637390191185943 35.0 29.0 38.5 2.0 40.0 66-67 25.242657201548248 28.5 18.0 36.0 2.0 39.0 68-69 17.64668259852894 13.0 7.0 29.5 2.0 35.5 70-71 16.49791523764888 14.5 7.5 26.5 2.0 33.5 72-73 18.64312094966752 19.5 7.0 30.0 2.0 35.0 74-75 19.321419386491417 18.5 7.5 31.0 2.0 35.5 76-77 20.214327489692188 23.0 7.5 31.5 2.0 34.5 78-79 23.215745566912563 28.5 11.5 34.0 2.0 36.0 80-81 25.834140717142425 31.5 21.5 35.0 2.0 36.0 82-83 26.8585704918363 33.0 25.5 35.0 2.0 36.0 84-85 26.862498304961598 33.0 26.0 35.0 2.0 36.0 86-87 26.574698229210917 33.0 25.0 35.0 2.0 36.0 88-89 26.145414511974614 33.0 24.5 35.0 2.0 35.0 90-91 24.491428411109666 31.5 11.0 34.5 2.0 35.0 92-93 22.35656809846006 31.0 2.0 34.0 2.0 35.0 94-95 18.041599209833343 23.0 2.0 33.0 2.0 35.0 96-97 16.764024756732766 15.0 2.0 32.5 2.0 35.0 98-99 15.71008386888154 2.0 2.0 32.0 2.0 34.0 100-101 15.08566840984356 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37676988849491266 1101 2 0.41258495503585735 1101 3 0.3984668058751204 1101 4 0.34523628574790877 1101 5 0.34708273873077644 1101 6 0.48167419045987714 1101 7 0.37923730850860693 1101 8 0.49040917247261007 1101 9 0.5519036762769076 1101 10-11 0.5223952267938472 1101 12-13 0.5195884892264502 1101 14-15 0.5276795970945543 1101 16-17 0.5614891843941692 1101 18-19 0.6035246495303213 1101 20-21 0.6437211577219628 1101 22-23 0.677029957564848 1101 24-25 0.6733038832006031 1101 26-27 0.7838703110993492 1101 28-29 0.7652561754055043 1101 30-31 0.8089094744566552 1101 32-33 0.720579177134816 1101 34-35 0.87316020473245 1101 36-37 0.8172393743989019 1101 38-39 0.8882168380635562 1101 40-41 0.8060635246123198 1101 42-43 0.8669528579269326 1101 44-45 0.7564863681612266 1101 46-47 0.5419180644806119 1101 48-49 0.6428493339567112 1101 50-51 0.5585747496900737 1101 52-53 0.5936659597657155 1101 54-55 0.47264833042358845 1101 56-57 1.2118466151573983 1101 58-59 -8.35180273739445 1101 60-61 -9.461899927506238 1101 62-63 -2.0904777259927023 1101 64-65 0.3809001842565678 1101 66-67 -0.7699371518954621 1101 68-69 -3.5831981639273884 1101 70-71 -4.050994610425551 1101 72-73 -6.5117960236098344 1101 74-75 -7.510261642593715 1101 76-77 -8.44368928531451 1101 78-79 -11.554953657665349 1101 80-81 -7.547763811741426 1101 82-83 -3.6180157817980962 1101 84-85 -2.7088090180279423 1101 86-87 -2.572852421163237 1101 88-89 -2.6353785865786854 1101 90-91 -8.626679885153653 1101 92-93 -14.521252683735028 1101 94-95 -12.592613502040619 1101 96-97 -12.3086597189319 1101 98-99 -11.853889849049168 1101 100-101 -11.632279700932102 1102 1 0.44648584451111617 1102 2 0.5283354216302918 1102 3 0.4762952807320495 1102 4 0.3814234405812513 1102 5 0.4391303674342595 1102 6 0.47584406428764936 1102 7 0.4751510541382302 1102 8 0.4841741827489585 1102 9 0.6296445331083689 1102 10-11 0.6524794334057944 1102 12-13 0.5908252438274815 1102 14-15 0.7256340917058139 1102 16-17 0.8266277016650463 1102 18-19 0.7804078055452592 1102 20-21 0.7106324266062103 1102 22-23 0.7175506771796663 1102 24-25 0.7333510194225141 1102 26-27 0.9718502331443375 1102 28-29 0.9607045669092429 1102 30-31 0.9216820660817859 1102 32-33 0.7135855518582233 1102 34-35 0.6590504254911878 1102 36-37 0.8858363230613335 1102 38-39 0.760657168481373 1102 40-41 0.8972293973686405 1102 42-43 1.062845484198725 1102 44-45 0.8524500674443232 1102 46-47 0.8974816839288948 1102 48-49 0.9878430334241557 1102 50-51 0.9011082032763014 1102 52-53 0.870766271936219 1102 54-55 0.7803906005224164 1102 56-57 1.4642578745971093 1102 58-59 -8.74426947611272 1102 60-61 -9.70729252794779 1102 62-63 -2.601462864382526 1102 64-65 0.2632438549238536 1102 66-67 -0.9362287678972869 1102 68-69 -3.323111110298557 1102 70-71 -3.754889834742489 1102 72-73 -6.417739666330382 1102 74-75 -7.146592445472955 1102 76-77 -8.257654597224441 1102 78-79 -11.219887308062145 1102 80-81 -7.0234801915102665 1102 82-83 -2.787192079051234 1102 84-85 -1.8527500722616779 1102 86-87 -1.5601332423967484 1102 88-89 -1.7082596015276827 1102 90-91 -7.620774771606381 1102 92-93 -13.980432697357925 1102 94-95 -12.24788083601527 1102 96-97 -12.13938544879574 1102 98-99 -11.708500707812778 1102 100-101 -11.540578789272235 1103 1 0.3435029991347456 1103 2 0.311039146619013 1103 3 0.2407676484089727 1103 4 0.27015398927429146 1103 5 0.216053403261526 1103 6 0.1951149870594051 1103 7 0.2449403858657746 1103 8 0.30360148681631927 1103 9 0.3278819044982981 1103 10-11 0.3069253101370393 1103 12-13 0.4273111599707704 1103 14-15 0.541656496436481 1103 16-17 0.4878085826969638 1103 18-19 0.3821315105532008 1103 20-21 0.3759995792073383 1103 22-23 0.37363676397624346 1103 24-25 0.3813087132830404 1103 26-27 0.28118342604741997 1103 28-29 0.5394103800347381 1103 30-31 0.5217022147699453 1103 32-33 0.5662465143091566 1103 34-35 0.4711055993155995 1103 36-37 0.22880851405563618 1103 38-39 0.2770081342064685 1103 40-41 0.3292181873712323 1103 42-43 0.5133522923946643 1103 44-45 0.5420234266046151 1103 46-47 0.4094524469612466 1103 48-49 0.3307437881604329 1103 50-51 0.4138798003422295 1103 52-53 0.386645956792421 1103 54-55 0.4102076824886538 1103 56-57 1.0133660280232561 1103 58-59 -6.122879888252612 1103 60-61 -8.26259197637745 1103 62-63 -2.0648943691460886 1103 64-65 0.04686266616651835 1103 66-67 -1.6417497374155303 1103 68-69 -3.990982424290676 1103 70-71 -4.829502563981517 1103 72-73 -7.529989839769373 1103 74-75 -8.212513152752967 1103 76-77 -9.034987552006662 1103 78-79 -11.92109241331233 1103 80-81 -8.452163070238822 1103 82-83 -4.5910340675435855 1103 84-85 -3.558156865880349 1103 86-87 -3.388864508505204 1103 88-89 -3.4623269271480375 1103 90-91 -8.893497340197783 1103 92-93 -14.491081355371339 1103 94-95 -12.695999414097924 1103 96-97 -12.47129150706361 1103 98-99 -12.035649309730415 1103 100-101 -11.726072802287984 1104 1 0.2104927804672272 1104 2 0.2271310547906218 1104 3 0.22446830860004852 1104 4 0.11321932359160058 1104 5 0.08874489524016838 1104 6 0.14124791616698218 1104 7 0.1390824366466319 1104 8 0.15037067258065662 1104 9 0.21598716275733665 1104 10-11 0.26048256326347285 1104 12-13 0.23718543778289813 1104 14-15 0.27591895064528416 1104 16-17 0.3277105845452297 1104 18-19 0.3167709318721208 1104 20-21 0.2652071504620963 1104 22-23 0.389603681289465 1104 24-25 0.33345649502772545 1104 26-27 0.2048166709563688 1104 28-29 0.20595405037799708 1104 30-31 0.3092553192636345 1104 32-33 0.2587972586031313 1104 34-35 0.2522874942567981 1104 36-37 0.34427048159790985 1104 38-39 0.5521785007355149 1104 40-41 0.5419173915517774 1104 42-43 0.6204039707958415 1104 44-45 0.4260854300485164 1104 46-47 0.3633992992526345 1104 48-49 0.33088373803112603 1104 50-51 0.36392102073169497 1104 52-53 0.2526810207172687 1104 54-55 -0.028773509046537527 1104 56-57 0.8515726751434869 1104 58-59 -6.345626894788037 1104 60-61 -8.492808183968652 1104 62-63 -2.438698146795332 1104 64-65 -0.17561208634493397 1104 66-67 -1.5136437478270004 1104 68-69 -4.326406362699435 1104 70-71 -4.6604959753676525 1104 72-73 -7.625240851995873 1104 74-75 -8.382308579949115 1104 76-77 -9.051857328818198 1104 78-79 -12.032815625045512 1104 80-81 -8.035416289750692 1104 82-83 -4.099578655372934 1104 84-85 -3.146371491140467 1104 86-87 -2.940685186514127 1104 88-89 -3.168272303483544 1104 90-91 -8.852748990675472 1104 92-93 -15.08381659415042 1104 94-95 -12.643052821096083 1104 96-97 -12.430342374353902 1104 98-99 -11.796207713038683 1104 100-101 -11.400746862030953 1105 1 0.1351281384014129 1105 2 0.1573643253585999 1105 3 0.07225774447771371 1105 4 0.15660426888538836 1105 5 0.1195298495130146 1105 6 0.13070703373714565 1105 7 0.13506272497993876 1105 8 0.21856765192090677 1105 9 0.23995199655238508 1105 10-11 0.199990537456614 1105 12-13 0.26153795548351866 1105 14-15 0.4402315643689789 1105 16-17 0.32637706282780243 1105 18-19 0.3116658109057582 1105 20-21 0.3325700019987039 1105 22-23 0.31349404174570594 1105 24-25 0.26758614730061225 1105 26-27 0.2477147666044317 1105 28-29 0.2832934243265939 1105 30-31 0.22575169198948686 1105 32-33 0.1774418702457936 1105 34-35 0.2608083533939052 1105 36-37 0.11831282576508073 1105 38-39 0.13274874995710917 1105 40-41 0.2793535808408194 1105 42-43 0.08089322365923834 1105 44-45 0.23817414311224638 1105 46-47 0.1860815806720595 1105 48-49 0.17129952109715418 1105 50-51 0.3197996608802143 1105 52-53 0.24147687661928785 1105 54-55 0.22135298999349828 1105 56-57 0.9943650028050541 1105 58-59 -6.582873071427176 1105 60-61 -8.9136312728164 1105 62-63 -2.6687102421106417 1105 64-65 -0.3660677469789064 1105 66-67 -1.5583772679598944 1105 68-69 -4.055242994074895 1105 70-71 -4.692515021527917 1105 72-73 -6.0671153353700955 1105 74-75 -6.360513640438896 1105 76-77 -8.452746123221607 1105 78-79 -11.849445759282386 1105 80-81 -7.825110579335782 1105 82-83 -3.8182582589212437 1105 84-85 -2.8681504532145254 1105 86-87 -2.605711767157924 1105 88-89 -2.7236683194240108 1105 90-91 -8.264760985636638 1105 92-93 -11.42626560500187 1105 94-95 -11.645649748674723 1105 96-97 -12.109193662716255 1105 98-99 -11.679582200374254 1105 100-101 -11.199322757493626 1106 1 0.11683897781540509 1106 2 0.07721419707968735 1106 3 0.12175576661550735 1106 4 0.04758877167760289 1106 5 0.03529439102882037 1106 6 0.030792624494310417 1106 7 0.04159469633102475 1106 8 0.08081397906416754 1106 9 0.0362847288114736 1106 10-11 0.0938332702705793 1106 12-13 0.09544417235329661 1106 14-15 0.2506854312725366 1106 16-17 0.2385857546992014 1106 18-19 0.24256534751477687 1106 20-21 0.07838120160155171 1106 22-23 0.07182922244192724 1106 24-25 0.19445899128069755 1106 26-27 0.27318847110267086 1106 28-29 0.16272270580116555 1106 30-31 0.1212412360049342 1106 32-33 0.1851952991702106 1106 34-35 0.2700711470408308 1106 36-37 0.2922724209232186 1106 38-39 0.24235745839445855 1106 40-41 0.14026102782641914 1106 42-43 0.1702093768305062 1106 44-45 0.15977168266613972 1106 46-47 0.23442702260906856 1106 48-49 0.21713192404760662 1106 50-51 0.21204162136693583 1106 52-53 0.09600562752765995 1106 54-55 -0.1229759052906445 1106 56-57 0.7105988057661676 1106 58-59 -6.265966792636128 1106 60-61 -8.976728253604453 1106 62-63 -2.444128257664943 1106 64-65 -0.11700735550238761 1106 66-67 -1.34428846104894 1106 68-69 -3.959317414340255 1106 70-71 -4.713172403040474 1106 72-73 -6.299541352172476 1106 74-75 -6.64654271181001 1106 76-77 -8.260742640082556 1106 78-79 -11.792717891516219 1106 80-81 -7.79684183936951 1106 82-83 -3.763687114720522 1106 84-85 -2.7833273456937135 1106 86-87 -2.6916367920135578 1106 88-89 -2.8915588249080635 1106 90-91 -8.057052282104738 1106 92-93 -11.164783161293274 1106 94-95 -11.733690417285164 1106 96-97 -12.13491116550338 1106 98-99 -11.609132469597796 1106 100-101 -11.328580281130266 1107 1 0.005472936567375797 1107 2 0.08588173379767383 1107 3 0.031975202633365285 1107 4 -0.03049833423042969 1107 5 0.0012186121442638864 1107 6 0.051680583358660215 1107 7 0.08576589330584028 1107 8 0.02950429856927883 1107 9 -0.08715017470331787 1107 10-11 0.008013576666876077 1107 12-13 0.09029080668190659 1107 14-15 0.05667803504488944 1107 16-17 -0.006191035455131555 1107 18-19 0.11208389889252146 1107 20-21 0.12270494775022911 1107 22-23 0.05187739975300332 1107 24-25 0.16393721902467462 1107 26-27 0.2713086801154958 1107 28-29 0.22131715345971514 1107 30-31 0.30837930270236313 1107 32-33 0.3263259068527802 1107 34-35 0.2738220891787293 1107 36-37 0.16756607643407762 1107 38-39 0.20230416577255994 1107 40-41 0.09938075116171063 1107 42-43 0.16212242393331877 1107 44-45 0.22894115826895955 1107 46-47 0.28322864431297035 1107 48-49 0.36180552421603096 1107 50-51 0.42200659771224736 1107 52-53 0.4258977337773331 1107 54-55 0.4541201915873785 1107 56-57 1.1620762969476033 1107 58-59 -6.2933005971373355 1107 60-61 -8.591857834003868 1107 62-63 -2.1166156679062773 1107 64-65 0.3178648975902121 1107 66-67 -1.3049163304969227 1107 68-69 -3.866751987035048 1107 70-71 -4.632295025667455 1107 72-73 -5.8316375532561615 1107 74-75 -6.512585146993429 1107 76-77 -8.998319214755412 1107 78-79 -12.159032184760447 1107 80-81 -7.773735289212684 1107 82-83 -3.71891950521686 1107 84-85 -2.754055381844374 1107 86-87 -2.5732399684131657 1107 88-89 -2.600621498941411 1107 90-91 -7.991422633246163 1107 92-93 -11.342735432989883 1107 94-95 -11.544492705905299 1107 96-97 -12.22780022393532 1107 98-99 -11.747099973787787 1107 100-101 -11.220436766982962 1108 1 0.030294765534954138 1108 2 -0.01958394338956637 1108 3 -0.020329495824547905 1108 4 0.045137304291785085 1108 5 -0.16885772725267145 1108 6 0.008678246978817583 1108 7 0.03397270706047095 1108 8 0.04055771947272291 1108 9 9.336505252690586E-4 1108 10-11 0.03238446078361079 1108 12-13 -0.033044694325425894 1108 14-15 0.06821673047786447 1108 16-17 0.06344524650334193 1108 18-19 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1.4697565989839632 2312 86-87 1.20526959092361 2312 88-89 1.3801002844326717 2312 90-91 2.9749034997494945 2312 92-93 4.992022710216528 2312 94-95 8.821001747431836 2312 96-97 9.414951084386411 2312 98-99 9.797857026098923 2312 100-101 9.017295318511112 2313 1 -0.2919055471520622 2313 2 -0.2751361694858119 2313 3 -0.3405319213074378 2313 4 -0.27091380742314897 2313 5 -0.2811470931007136 2313 6 -0.17912738257256677 2313 7 -0.30786915829450123 2313 8 -0.29147024713901004 2313 9 -0.3427216767930332 2313 10-11 -0.4482826316588202 2313 12-13 -0.415789284729982 2313 14-15 -0.6141873451189284 2313 16-17 -0.4274282963997038 2313 18-19 -0.5371099065163776 2313 20-21 -0.5563458353632669 2313 22-23 -0.4950429845472897 2313 24-25 -0.43909019581847986 2313 26-27 -0.5476318437851901 2313 28-29 -0.6062261118581844 2313 30-31 -0.5942929383179845 2313 32-33 -0.5067561511492116 2313 34-35 -0.4731096008340643 2313 36-37 -0.6714872093683013 2313 38-39 -0.9063472539498321 2313 40-41 -1.0989915617186838 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0.9670599940022058 2314 66-67 1.5551600481218522 2314 68-69 -0.43833328023602647 2314 70-71 2.5377641035923517 2314 72-73 8.11160192600914 2314 74-75 9.602213858741287 2314 76-77 8.471210907563869 2314 78-79 6.081371944978141 2314 80-81 3.0910081914073864 2314 82-83 1.446226260954358 2314 84-85 1.0970969693172883 2314 86-87 1.0669204936267214 2314 88-89 1.1813821353491285 2314 90-91 2.4332535035003353 2314 92-93 4.617454057269615 2314 94-95 8.552544469544308 2314 96-97 9.219537585099067 2314 98-99 9.332347598977817 2314 100-101 8.551219357669924 2315 1 -0.656069314416353 2315 2 -0.5185618240070511 2315 3 -0.5332694929653528 2315 4 -0.42178228127904305 2315 5 -0.6999815406653767 2315 6 -0.7167812573189636 2315 7 -0.5915889663732443 2315 8 -0.7462160000744973 2315 9 -0.993866402720819 2315 10-11 -0.8572995168530255 2315 12-13 -0.6710287778549997 2315 14-15 -0.8632646749106101 2315 16-17 -0.9072683366414438 2315 18-19 -0.8562104754463178 2315 20-21 -0.7812612834903874 2315 22-23 -0.8404501584344288 2315 24-25 -0.8399388328138855 2315 26-27 -0.8918997523710814 2315 28-29 -0.9284983825038182 2315 30-31 -0.8300223301622935 2315 32-33 -0.9231826527263536 2315 34-35 -1.0159365774417992 2315 36-37 -1.0910082611815923 2315 38-39 -1.0864376924659638 2315 40-41 -0.9178486716154808 2315 42-43 -1.0232825928642129 2315 44-45 -1.0060494675516622 2315 46-47 -1.1511276722146917 2315 48-49 -0.9132060281114391 2315 50-51 -0.8846496803415818 2315 52-53 -0.8788199246252617 2315 54-55 -0.967247305421914 2315 56-57 0.37832229604241263 2315 58-59 3.717037802934861 2315 60-61 3.36249537476111 2315 62-63 0.9829063609063482 2315 64-65 0.8246059605671903 2315 66-67 1.7309717126914776 2315 68-69 6.177359752640292 2315 70-71 5.165821351224093 2315 72-73 9.067253457234916 2315 74-75 9.833324107693151 2315 76-77 8.569314827867078 2315 78-79 5.831148167258139 2315 80-81 2.7128862334688897 2315 82-83 1.1863179909578072 2315 84-85 0.5992994411929686 2315 86-87 0.7371558031296352 2315 88-89 0.8133572583470752 2315 90-91 2.0596288151544613 2315 92-93 4.014706471186532 2315 94-95 7.959539073343127 2315 96-97 8.744981406880113 2315 98-99 8.886736708533048 2315 100-101 8.040731161448926 2316 1 -0.5803224323612497 2316 2 -0.5779359700883404 2316 3 -0.6470185522848482 2316 4 -0.45963657991496376 2316 5 -0.5255321036124343 2316 6 -0.5893417348558785 2316 7 -0.6472965407691973 2316 8 -0.6857102610101364 2316 9 -0.8031044332463608 2316 10-11 -0.6696055905439309 2316 12-13 -0.7602891655249664 2316 14-15 -0.8164967435309691 2316 16-17 -1.069375022596624 2316 18-19 -1.044464060991622 2316 20-21 -0.8417253768935709 2316 22-23 -0.9289483574422306 2316 24-25 -2.214650856719956 2316 26-27 -1.3263757841971326 2316 28-29 -1.1250223579840153 2316 30-31 -0.7426739897784245 2316 32-33 -0.6683331250758258 2316 34-35 -0.7945143415812268 2316 36-37 -0.7103845991397932 2316 38-39 -0.9268781135985762 2316 40-41 -0.9138534645196401 2316 42-43 -1.003832169466655 2316 44-45 -0.9351893019918975 2316 46-47 -0.9110931866847309 2316 48-49 -1.041235311586881 2316 50-51 -1.2399708999018983 2316 52-53 -1.0488425009573774 2316 54-55 -0.9092072817496373 2316 56-57 0.17349023034455513 2316 58-59 3.580067561316966 2316 60-61 3.4981892133951327 2316 62-63 0.9454503993664787 2316 64-65 0.5301039184685408 2316 66-67 5.595827889035281 2316 68-69 8.723149230169899 2316 70-71 5.5838341956227495 2316 72-73 9.14681858885712 2316 74-75 9.91585894027029 2316 76-77 8.499814794988925 2316 78-79 5.982747579835131 2316 80-81 2.9769739377700866 2316 82-83 1.4637879805098635 2316 84-85 1.161599012315719 2316 86-87 1.0259652486578439 2316 88-89 1.0529850776487386 2316 90-91 2.4820921925445205 2316 92-93 4.311655378535892 2316 94-95 8.41454407812872 2316 96-97 9.068659668575604 2316 98-99 9.466629160439615 2316 100-101 8.52788325578731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 27.0 4 69.0 5 146.0 6 322.0 7 785.0 8 1711.0 9 3052.0 10 5226.0 11 8070.0 12 11379.0 13 15277.0 14 20035.0 15 27896.0 16 37487.0 17 49009.0 18 49349.0 19 46927.0 20 48231.0 21 47808.0 22 46253.0 23 44876.0 24 46963.0 25 49779.0 26 64911.0 27 93424.0 28 131119.0 29 173446.0 30 207000.0 31 221879.0 32 227325.0 33 235993.0 34 242476.0 35 238934.0 36 203520.0 37 132828.0 38 44548.0 39 2088.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.36111885606109 20.137617467906228 15.126624835542232 22.374638840490448 2 15.432426054914398 21.498318726807856 39.97210827485687 23.097146943420878 3 18.542849116334885 26.501644383646592 30.488631756981572 24.466874743036954 4 12.650363844264367 17.896799947341407 37.45051117322555 32.002325035168674 5 12.953089075185037 38.58230647299929 33.93958764341132 14.525016808404345 6 30.342752051760808 37.466373495462335 17.814395003476402 14.376479449300458 7 27.648890914342385 33.881812390811646 20.88952737833221 17.579769316513758 8 26.32308551137917 35.19247757689463 19.690608625090285 18.793828286635915 9 26.915231534152728 16.54483372077925 20.262336192747718 36.277598552320306 10-11 24.642261470778976 27.48312784851878 27.18230124528222 20.692309435420025 12-13 25.84767206932806 24.92754947264073 27.92860372358123 21.29617473444998 14-15 22.629877516372666 25.78914759632288 26.825002450391928 24.755972436912526 16-17 21.37530650071057 29.428870073912666 27.212695037323215 21.98312838805355 18-19 21.388786675774124 27.32331009348813 30.362025064214414 20.92587816652333 20-21 22.462073403345766 26.340393205746548 30.303635780938816 20.893897609968874 22-23 21.988469782276137 26.621940433203257 30.079980634298657 21.30960915022195 24-25 21.608025831486028 26.785563344439357 30.333328177778863 21.27308264629575 26-27 21.45443571636421 27.153131947639192 30.1628568890187 21.229575446977904 28-29 21.195115169613366 27.225077217973197 30.376509333959685 21.203298278453747 30-31 21.38527785037126 26.86690596435427 30.4066759702231 21.341140215051364 32-33 21.173779254109018 27.01449374407634 30.142563480944478 21.669163520870164 34-35 21.65948568500666 27.020519976569563 30.001318276801797 21.31867606162198 36-37 21.72056817760713 26.767765803928285 29.94013634909802 21.57152966936657 38-39 21.316934270933153 26.984195227321667 30.152902932299003 21.54596756944618 40-41 21.506569425001267 26.856498026420173 30.154450475576795 21.482482073001766 42-43 21.498279690052787 26.784900273112473 29.918336097078512 21.79848393975623 44-45 21.430532264383988 27.00513451153781 29.7183915965413 21.8459416275369 46-47 21.520985094028745 27.243518765541857 29.18871212545057 22.04678401497883 48-49 21.5028543190048 27.318548405231446 29.06810842472497 22.11048885103878 50-51 21.498216233641013 27.378960767213812 28.896601273892685 22.22622172525249 52-53 21.76419575936378 27.482271321498203 28.685930904248497 22.067602014889516 54-55 21.387275231100418 27.295463179343134 28.867784227612326 22.44947736194412 56-57 21.5008651313692 27.633102883807254 28.710095072587816 22.155936912235727 58-59 20.16244688128656 27.06012560075598 30.26933289273063 22.508094625226825 60-61 21.496258056043562 27.323181039302003 28.9638386083616 22.216722296292836 62-63 21.332266729588614 27.296609120900783 28.92933654273633 22.441787606774277 64-65 20.54799577802618 27.144730825757495 29.420479886645655 22.886793509570666 66-67 20.80568971826166 27.20791723953347 29.248541571214187 22.73785147099068 68-69 21.50315531165674 26.645135102592874 29.11117570539295 22.740533880357436 70-71 20.69414963151676 27.0888418413083 29.395539029495747 22.821469497679193 72-73 20.568204600527483 26.959471892327848 29.407892869795756 23.064430637348917 74-75 20.976186220654505 27.008015943159048 28.782305810048054 23.233492026138396 76-77 19.379171279326563 27.389136624607453 29.638357161596513 23.593334934469475 78-79 20.22431921474732 27.466148076322344 28.623171526645468 23.68636118228487 80-81 20.602179653236657 27.57149120898133 28.207640044644798 23.61868909313721 82-83 21.02318996549849 27.49619807148345 27.743952883422875 23.736659079595192 84-85 20.872909707417488 27.09129971408254 27.805697702984673 24.2300928755153 86-87 20.865029704978923 27.08116051659488 27.962265243618955 24.091544534807245 88-89 20.869640283111927 26.995141488522744 27.869349833508533 24.265868394856792 90-91 20.58175224974362 27.189032533644774 27.955594485627415 24.27362073098419 92-93 20.378195653290028 26.93014621439554 28.201805229418703 24.489852902895734 94-95 19.28240955694785 26.902367569595036 28.743219843667212 25.0720030297899 96-97 19.117451909293212 26.796328404803837 28.943383346758228 25.14283633914472 98-99 19.271366884320667 26.755863530541347 28.474441663195655 25.498327921942334 100-101 19.024909049319632 26.74192826816292 28.762657314301464 25.470505368215978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 835.0 1 621.0 2 445.0 3 536.5 4 608.5 5 716.5 6 970.5 7 1512.5 8 2182.0 9 2777.0 10 3150.0 11 3441.0 12 3871.0 13 4361.0 14 4829.5 15 5115.0 16 5306.5 17 5564.0 18 5991.5 19 6475.5 20 7027.0 21 7941.5 22 9185.0 23 10420.0 24 11893.5 25 14244.0 26 17395.5 27 21012.0 28 24843.0 29 29047.0 30 34127.0 31 39507.0 32 45685.0 33 52401.0 34 58561.0 35 64531.5 36 70602.0 37 76326.0 38 80889.5 39 84382.0 40 88162.0 41 92932.5 42 97511.5 43 102010.0 44 105758.0 45 108041.0 46 109330.0 47 109961.5 48 110709.5 49 109417.0 50 105768.5 51 101262.0 52 96236.5 53 90768.0 54 84264.5 55 76855.5 56 68773.5 57 60517.5 58 52252.0 59 44127.5 60 36114.0 61 28521.0 62 21961.0 63 16463.0 64 12093.5 65 8939.5 66 6609.0 67 4746.5 68 3198.0 69 2067.0 70 1508.0 71 1202.0 72 969.5 73 751.5 74 549.0 75 378.5 76 230.5 77 131.0 78 75.5 79 43.5 80 20.0 81 13.5 82 8.0 83 2.0 84 3.5 85 2.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02100588704371994 2 0.004927750898954849 3 0.003740774405046017 4 0.0 5 0.06478014137969111 6 0.0 7 0.0 8 1.7984492331952004E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9782941565147204E-4 16-17 0.0 18-19 0.0012229454785727364 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.193796932780802E-5 28-29 0.0020142631411786244 30-31 0.008362788934357683 32-33 2.8775187731123206E-4 34-35 0.0010251160629212643 36-37 0.024818599418093765 38-39 0.023901390309164213 40-41 0.025394103172716228 42-43 0.026329296773977735 44-45 0.02683286255927239 46-47 0.028181699484168793 48-49 0.028361544407488313 50-51 0.02377549886284055 52-53 0.02037642981210162 54-55 0.015430694420814819 56-57 0.1544508201468038 58-59 0.7307818614165377 60-61 0.3028048973930759 62-63 3.2372086197513607E-4 64-65 0.1347578010433164 66-67 1.357919093524036 68-69 2.614045960449224 70-71 2.115497848515182 72-73 1.7729292385761604 74-75 1.4906806159185058 76-77 1.080598221765336 78-79 0.4002628613399238 80-81 2.1581390798342405E-4 82-83 1.7984492331952004E-4 84-85 0.0 86-87 0.0030034102194359848 88-89 1.7984492331952004E-5 90-91 0.21466290047417913 92-93 0.16637453856288797 94-95 1.2268301289164378 96-97 1.2017417621133648 98-99 0.9500847609123606 100-101 0.38405883374883504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2780173.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.94820493292131 #Duplication Level Percentage of deduplicated Percentage of total 1 69.34428554262738 29.78212586411773 2 14.666507257747922 12.598003187118708 3 5.984049106555768 7.710125020710653 4 3.0526531947261724 5.244238999849463 5 1.7726327538266764 3.8065697391078372 6 1.12855786976354 2.9081720801559374 7 0.7714547679341587 2.319281822880085 8 0.5603743142946207 1.9253656711576503 9 0.39051415309481763 1.5094693688690188 >10 2.059292108419314 15.891783511520375 >50 0.16286832087927725 4.888351504956487 >100 0.09992733764931669 7.759497704142381 >500 0.00570807961852414 1.641219018309259 >1k 9.233658206395498E-4 0.6213876416721108 >5k 8.394234733086817E-5 0.33200039406814047 >10k+ 1.6788469466173634E-4 1.0624084713643207 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16581 0.5964017347121924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12888 0.4635682743483948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9209 0.331238379769892 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2882 0.10366261380137134 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7984492331952004E-5 0.0 16-17 0.0 0.0 0.0 5.395347699585601E-5 0.0 18-19 0.0 0.0 0.0 7.193796932780802E-5 0.0 20-21 0.0 0.0 0.0 2.5178289264732805E-4 0.0 22-23 0.0 0.0 0.0 7.193796932780802E-4 0.0 24-25 0.0 0.0 0.0 0.0017085267715354403 0.0 26-27 0.0 0.0 0.0 0.00420837120567677 0.0 28-29 0.0 0.0 0.0 0.012337361739719076 0.0 30-31 0.0 0.0 0.0 0.03825301519006191 0.0 32-33 0.0 0.0 0.0 0.09056990338371029 0.0 34-35 0.0 0.0 0.0 0.16428833745238158 0.0 36-37 0.0 0.0 0.0 0.2847124981071322 0.0 38-39 0.0 0.0 0.0 0.47110377663548275 0.0 40-41 0.0 0.0 0.0 0.6857666771096619 0.0 42-43 0.0 0.0 0.0 0.8965808962248032 0.0 44-45 0.0 0.0 0.0 1.137645031442288 0.0 46-47 0.0 0.0 0.0 1.4123401673205227 0.0 48-49 0.0 0.0 0.0 1.691819178159057 0.0 50-51 0.0 0.0 0.0 1.9783121410070525 0.0 52-53 0.0 0.0 0.0 2.245309914167212 0.0 54-55 1.7984492331952004E-5 0.0 0.0 2.52482489399041 0.0 56-57 3.596898466390401E-5 0.0 0.0 2.7724713534013894 0.0 58-59 3.596898466390401E-5 0.0 0.0 2.9495106959171244 0.0 60-61 3.596898466390401E-5 0.0 0.0 3.0877395039805076 0.0 62-63 3.596898466390401E-5 0.0 0.0 3.205609866724121 0.0 64-65 3.596898466390401E-5 3.596898466390401E-5 0.0 3.32758069371942 0.0 66-67 3.596898466390401E-5 7.193796932780802E-5 0.0 3.4293909048106 0.0 68-69 3.596898466390401E-5 7.193796932780802E-5 0.0 3.5448693300740635 0.0 70-71 3.596898466390401E-5 7.193796932780802E-5 0.0 3.752302464630798 0.0 72-73 3.596898466390401E-5 7.193796932780802E-5 0.0 4.008599464853447 0.0 74-75 3.596898466390401E-5 7.193796932780802E-5 0.0 4.296603125057326 0.0 76-77 3.596898466390401E-5 7.193796932780802E-5 0.0 4.698556528676453 0.0 78-79 3.596898466390401E-5 7.193796932780802E-5 0.0 5.265175944086932 0.0 80-81 3.596898466390401E-5 7.193796932780802E-5 0.0 5.912239993698234 0.0 82-83 3.596898466390401E-5 7.193796932780802E-5 0.0 6.514990254203605 0.0 84-85 3.596898466390401E-5 7.193796932780802E-5 0.0 6.996920695222924 0.0 86-87 3.596898466390401E-5 7.193796932780802E-5 0.0 7.420635334563713 0.0 88-89 3.596898466390401E-5 7.193796932780802E-5 0.0 7.853414158039806 3.596898466390401E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8115 0.0 49.650692 1 GTATCAA 14785 0.0 42.18578 1 TATCAAC 19035 0.0 32.659466 2 ATCAACG 19030 0.0 32.54236 3 TCAACGC 19125 0.0 32.4042 4 CAACGCA 19485 0.0 31.805511 5 AACGCAG 20040 0.0 30.99817 6 GTACATG 16385 0.0 27.182007 1 ACGCAGA 22960 0.0 26.912197 7 CGCAGAG 22905 0.0 26.853355 8 TACATGG 16530 0.0 26.508759 2 ACATGGG 16595 0.0 25.266645 3 GCAGAGT 24320 0.0 24.670797 9 AGTACTT 13245 0.0 23.14822 12-13 GAGTACT 12990 0.0 22.985804 12-13 CATGGGA 11095 0.0 22.14715 4 GTACTTT 13850 0.0 21.303488 14-15 AGAGTAC 22835 0.0 21.03253 10-11 CATGGGG 5650 0.0 20.618446 4 CAGAGTA 23890 0.0 20.389786 10-11 >>END_MODULE