FastQCFastQC Report
Fri 27 May 2016
SRR939890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854688
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156830.5493770247396563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109620.3839999327422121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76890.26934642244616575No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30320.10621125671176676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82000.045.7819331
GTATCAA150450.042.8384251
TATCAAC189750.033.594072
ATCAACG193200.032.8492283
TCAACGC194650.032.6286964
CAACGCA199800.031.7887825
AACGCAG203850.031.4561466
ACGCAGA226600.027.965857
CGCAGAG228100.027.616468
GTACATG160800.027.1790811
TACATGG162850.026.1725222
GCAGAGT233150.025.0408529
ACATGGG168650.024.1017043
GAGTACT123850.022.88660612-13
AGTACTT126150.022.02180912-13
CATGGGA112400.021.8486464
GTACTTT132750.021.23506714-15
AGAGTAC218550.020.95475810-11
CATGGGG57450.020.5543654
ATGGGAG45650.020.1999765
Produced by FastQC (version 0.11.5)