FastQCFastQC Report
Fri 27 May 2016
SRR939890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854688
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165350.5792226681164456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133210.46663593359414407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93280.32676075283883915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78750.050.6790921
GTATCAA144400.041.4082371
TATCAAC190400.031.3471582
ATCAACG191950.031.0655043
TCAACGC193400.030.8325924
CAACGCA198450.030.0718925
AACGCAG203850.029.3218276
GTACATG172100.026.4987811
ACGCAGA232450.025.5712957
CGCAGAG232750.025.3752828
TACATGG177200.025.2483182
ACATGGG177200.024.7097993
GCAGAGT246550.023.3007749
GTGGTAT40100.022.7216681
GAGTACT128150.022.35885412-13
AGTACTT131650.022.2869212-13
CATGGGA114800.021.9837074
TGGTATC39650.021.6580752
CATGGGG59750.021.0396824
GTACTTT137450.020.7597514-15
Produced by FastQC (version 0.11.5)