FastQCFastQC Report
Fri 27 May 2016
SRR939889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029295
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105090.5178645785851737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75690.3729866776392787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.2639340263490522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.049.3371771
GTATCAA103050.043.0432051
TATCAAC136250.033.634242
ATCAACG136550.033.633153
TCAACGC137700.033.59514
CAACGCA140200.032.8260275
AACGCAG145300.031.7710156
ACGCAGA162350.028.2298567
CGCAGAG163950.027.838618
GTACATG119400.027.6134991
TACATGG121000.027.2483632
GCAGAGT167550.025.485479
ACATGGG123800.025.331723
GAGTACT89050.022.98984712-13
AGTACTT92600.022.44152512-13
AGAGTAC155850.021.8415210-11
CATGGGG46050.021.5338634
CATGGGA75650.021.4497624
GTACTTT95900.021.15217214-15
CAGAGTA162450.020.92494210-11
Produced by FastQC (version 0.11.5)