##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939889_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2029295 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.811071825436912 31.0 28.0 33.0 16.0 34.0 2 29.620512542533245 31.0 30.0 33.0 25.0 34.0 3 29.85273506316233 31.0 30.0 33.0 25.0 34.0 4 33.63894406678181 35.0 33.0 37.0 28.0 37.0 5 33.60376091204088 35.0 33.0 37.0 28.0 37.0 6 33.637112396176995 35.0 33.0 37.0 28.0 37.0 7 33.56023495844616 35.0 33.0 37.0 28.0 37.0 8 33.42565176576102 35.0 33.0 37.0 27.0 37.0 9 34.67044958963581 37.0 34.0 39.0 27.0 39.0 10-11 34.49162541670876 37.0 33.5 39.0 27.0 39.0 12-13 34.35617344939992 37.0 33.0 39.0 27.0 39.0 14-15 34.96216838852902 38.0 33.0 40.0 25.5 41.0 16-17 34.80877077999995 38.0 33.0 40.0 25.0 41.0 18-19 34.70178239240722 38.0 33.0 40.0 25.0 41.0 20-21 34.924773135497794 38.0 34.0 40.0 25.0 41.0 22-23 34.81677824071907 38.0 33.0 40.0 25.0 41.0 24-25 34.60359927955275 38.0 33.0 40.0 24.5 41.0 26-27 34.477612668439036 38.0 33.0 40.0 24.0 41.0 28-29 34.503573654890005 38.0 33.0 40.0 24.0 41.0 30-31 34.25362330267408 38.0 33.0 40.0 23.5 41.0 32-33 33.87698215390074 38.0 32.5 40.0 22.0 41.0 34-35 33.67161181592622 38.0 32.0 40.0 20.0 41.0 36-37 33.448100202287 37.0 32.0 40.0 19.0 41.0 38-39 33.214601129949074 37.0 31.0 40.0 18.0 41.0 40-41 32.95671895904735 37.0 31.0 40.0 16.0 41.0 42-43 32.527004945067134 37.0 31.0 40.0 15.0 41.0 44-45 32.120812153974654 37.0 30.0 40.0 10.5 41.0 46-47 31.662006016867927 36.5 30.0 39.0 8.5 41.0 48-49 31.25568781276256 36.0 29.5 39.0 7.5 40.0 50-51 30.625268134992695 35.5 28.5 38.5 4.0 40.0 52-53 30.78606240098162 35.5 29.0 39.0 2.0 40.0 54-55 30.870125585486583 36.0 29.0 39.0 2.0 40.0 56-57 30.57219798008668 36.0 28.0 39.0 2.0 40.0 58-59 30.181092941144584 35.0 28.0 39.0 2.0 40.0 60-61 29.772123816399294 35.0 27.0 38.5 2.0 40.0 62-63 29.31597574527114 34.5 26.0 38.0 2.0 40.0 64-65 28.770574509866726 34.0 26.0 38.0 2.0 40.0 66-67 28.16987426667882 34.0 25.0 37.5 2.0 39.5 68-69 27.606359351400364 33.0 23.5 37.0 2.0 39.0 70-71 27.036050451018703 32.5 22.5 36.0 2.0 39.0 72-73 26.378942933383268 32.0 21.5 36.0 2.0 38.5 74-75 26.004399557481783 32.0 20.0 35.0 2.0 38.0 76-77 25.42628794729204 31.5 18.0 35.0 2.0 37.5 78-79 24.743188890723133 31.0 15.0 35.0 2.0 37.0 80-81 23.99567017116782 30.5 7.5 34.0 2.0 36.0 82-83 23.235620005962662 30.0 4.0 34.0 2.0 35.5 84-85 22.42914435801596 29.0 2.0 34.0 2.0 35.0 86-87 21.46563806642208 29.0 2.0 34.0 2.0 35.0 88-89 20.451943408917877 27.5 2.0 33.5 2.0 35.0 90-91 19.044558085443466 25.5 2.0 32.5 2.0 34.5 92-93 17.49795175171673 20.5 2.0 32.0 2.0 34.0 94-95 16.98635141761055 19.5 2.0 31.0 2.0 34.0 96-97 16.656549934829584 18.5 2.0 31.5 2.0 34.0 98-99 15.766114586592881 4.0 2.0 31.0 2.0 34.0 100-101 14.172641483865085 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7632159547221349 1101 2 0.9611071416587329 1101 3 0.9403782823899149 1101 4 0.8704938778999178 1101 5 0.9695459583728692 1101 6 1.142439992757538 1101 7 0.9443910745919979 1101 8 1.120957683852751 1101 9 1.1496938964677241 1101 10-11 1.1806188820599886 1101 12-13 1.2958690067440983 1101 14-15 1.4597992978456702 1101 16-17 1.2826555098504784 1101 18-19 1.3561847767606636 1101 20-21 1.2895299455490417 1101 22-23 1.5996463255443416 1101 24-25 1.6429792108028565 1101 26-27 1.6449717609430863 1101 28-29 1.6073236062124678 1101 30-31 1.6688180800588626 1101 32-33 1.6573097868713234 1101 34-35 1.5544401044211824 1101 36-37 1.392052673420629 1101 38-39 1.2959049626541344 1101 40-41 1.359509818460083 1101 42-43 1.2469213665082393 1101 44-45 1.144913274425484 1101 46-47 0.9617093517625257 1101 48-49 1.113998811089214 1101 50-51 0.6554680110052473 1101 52-53 0.8071999757832913 1101 54-55 1.1600914893783916 1101 56-57 1.1308249216475978 1101 58-59 1.1281917055480086 1101 60-61 0.8995519681243884 1101 62-63 0.3694269275122508 1101 64-65 0.6510714804485822 1101 66-67 0.8727659554498537 1101 68-69 0.8159348350193021 1101 70-71 0.7608507849322272 1101 72-73 0.33117991065745045 1101 74-75 0.2228455790250976 1101 76-77 0.3574564448541686 1101 78-79 0.0041121316616354875 1101 80-81 -0.25608111426436864 1101 82-83 -0.44839417222449995 1101 84-85 -0.39678764605362815 1101 86-87 -0.9892917291077694 1101 88-89 -4.757558713785087 1101 90-91 -7.834456620432867 1101 92-93 -10.173492011998976 1101 94-95 -8.345371308831758 1101 96-97 -5.189839570753074 1101 98-99 -3.579530007101937 1101 100-101 -2.7589533492147673 1102 1 1.8670492428515963 1102 2 1.1701159960819787 1102 3 1.0826376271367621 1102 4 1.0005984556681042 1102 5 1.1189110746390654 1102 6 1.1356331202933418 1102 7 1.0355068536945282 1102 8 1.2179019945306422 1102 9 1.3173762664051267 1102 10-11 1.4556496022406549 1102 12-13 1.3823576162915217 1102 14-15 1.4883446188761766 1102 16-17 1.6672774763479552 1102 18-19 1.655756354781623 1102 20-21 1.6583171810833406 1102 22-23 1.542133857579124 1102 24-25 1.6908775537581633 1102 26-27 1.5814972254820319 1102 28-29 1.458855187111709 1102 30-31 1.558807544789694 1102 32-33 1.8597131105550133 1102 34-35 1.7844985096655748 1102 36-37 1.6851082059129894 1102 38-39 1.8158523232094197 1102 40-41 1.7920181350495312 1102 42-43 1.8260821059328336 1102 44-45 1.6232839147890346 1102 46-47 1.6125239900669932 1102 48-49 1.5750243116067502 1102 50-51 1.6056704136370357 1102 52-53 1.6548138111194355 1102 54-55 1.787638127778795 1102 56-57 1.731848650911477 1102 58-59 1.5517798687764142 1102 60-61 1.4981752796676098 1102 62-63 1.4220649884323073 1102 64-65 1.5502283360305213 1102 66-67 1.4709575239134196 1102 68-69 1.280891919019158 1102 70-71 1.1632913787450185 1102 72-73 1.1703895163874556 1102 74-75 1.0271243559006464 1102 76-77 1.1872345861079587 1102 78-79 1.0416472569132544 1102 80-81 1.1398475853955894 1102 82-83 0.9691894872224651 1102 84-85 1.0733279835993734 1102 86-87 0.962839218199278 1102 88-89 0.7114671797293575 1102 90-91 -5.09680950028836 1102 92-93 -9.850034340348813 1102 94-95 -7.703450993105793 1102 96-97 -4.143516674900944 1102 98-99 -2.499230868189523 1102 100-101 -1.5220684677872853 1103 1 2.036692583220706 1103 2 1.3164429795462524 1103 3 1.0450321319430707 1103 4 1.0121017255911084 1103 5 0.9948160883737387 1103 6 0.9205927446691504 1103 7 0.8970293974416848 1103 8 0.7877558006414915 1103 9 0.8313080933979791 1103 10-11 0.956433671348087 1103 12-13 0.9525039813808291 1103 14-15 0.9301837835077649 1103 16-17 0.9364180681261516 1103 18-19 0.8435750770099091 1103 20-21 0.8739116101197695 1103 22-23 0.9263331047402374 1103 24-25 0.7599044907222634 1103 26-27 0.8092674373173736 1103 28-29 0.8544443531239452 1103 30-31 0.8075812546308327 1103 32-33 0.8879447068142383 1103 34-35 0.9184928688965286 1103 36-37 0.8964731626766635 1103 38-39 0.9972121053102754 1103 40-41 0.7771228929780349 1103 42-43 0.6175219323911065 1103 44-45 0.6496635119274501 1103 46-47 0.5971255023718705 1103 48-49 0.8419002290463062 1103 50-51 0.7243814310850922 1103 52-53 0.6976090181777401 1103 54-55 0.6324734884533498 1103 56-57 0.5020679417451994 1103 58-59 0.7014445649844951 1103 60-61 0.6487897225546853 1103 62-63 0.6136802122130014 1103 64-65 0.5412407419670799 1103 66-67 0.5364948770264455 1103 68-69 0.4547814374828185 1103 70-71 0.38450697165534464 1103 72-73 0.3379371735409151 1103 74-75 0.2913499844537384 1103 76-77 0.21985967543578155 1103 78-79 -0.17325160245189153 1103 80-81 -0.4115698966808594 1103 82-83 -0.5674951168026148 1103 84-85 -0.6582809775691167 1103 86-87 -0.7214560943404145 1103 88-89 -1.4353964610021386 1103 90-91 -6.7935953830129385 1103 92-93 -10.173621414405584 1103 94-95 -8.63580401224818 1103 96-97 -5.846507044615969 1103 98-99 -4.487571861349249 1103 100-101 -3.700318205740592 1104 1 1.8813167384677314 1104 2 1.1435411384966692 1104 3 0.8919857566324652 1104 4 0.6908900194480907 1104 5 0.7424702544085306 1104 6 0.8462759139961875 1104 7 0.7085541213820861 1104 8 0.7583108409960531 1104 9 0.7474702552201649 1104 10-11 0.9033664938753603 1104 12-13 0.7700496849889191 1104 14-15 0.8181554049792297 1104 16-17 0.8566918599875919 1104 18-19 0.8834913512192131 1104 20-21 1.036751074966098 1104 22-23 0.9603438244927176 1104 24-25 0.8451412917736931 1104 26-27 0.8098373420164506 1104 28-29 0.668346300630283 1104 30-31 0.8009401701975847 1104 32-33 0.7521095000051048 1104 34-35 0.8717948102996544 1104 36-37 0.8653953614255911 1104 38-39 0.8176750035010514 1104 40-41 0.8207735396503963 1104 42-43 0.8421901751767962 1104 44-45 0.5873976090472297 1104 46-47 0.6564964571025946 1104 48-49 0.4633479337385289 1104 50-51 0.4721088589338507 1104 52-53 0.4416622903485212 1104 54-55 0.7188272701023948 1104 56-57 0.7537915744223476 1104 58-59 0.46954168895164017 1104 60-61 0.4099261588810812 1104 62-63 0.41290766696713277 1104 64-65 0.4707831433192915 1104 66-67 0.5292304265704182 1104 68-69 0.2930703676343782 1104 70-71 0.12898863325657928 1104 72-73 0.2876839951545733 1104 74-75 0.2786924628182099 1104 76-77 0.12444320939466991 1104 78-79 -0.10053197250208967 1104 80-81 -0.15004723432091893 1104 82-83 -0.05940813068648865 1104 84-85 -0.262752790173554 1104 86-87 -0.18339163186432472 1104 88-89 -0.39074565662469496 1104 90-91 -3.379285498817854 1104 92-93 -8.016936873719885 1104 94-95 -5.488674838741609 1104 96-97 -3.093327493537341 1104 98-99 -2.0934970299387743 1104 100-101 -1.4448733621027348 1105 1 1.8372482804718935 1105 2 1.0443917562548144 1105 3 0.9192216702028091 1105 4 0.764429316096269 1105 5 0.7733951123486378 1105 6 0.6569987531606571 1105 7 0.6773228320543723 1105 8 0.7007914101394022 1105 9 0.8200980243996625 1105 10-11 0.6844982283052516 1105 12-13 0.6436787457931956 1105 14-15 0.7357846710125173 1105 16-17 0.5383834318535179 1105 18-19 0.5348791653231544 1105 20-21 0.545152755208008 1105 22-23 0.5555233669261668 1105 24-25 0.7854907271114868 1105 26-27 0.5249931277799575 1105 28-29 0.4006621264228656 1105 30-31 0.6307606042286906 1105 32-33 0.6250867468861827 1105 34-35 0.5092131658692622 1105 36-37 0.6366326805322302 1105 38-39 0.4362876036207979 1105 40-41 0.12685295480419967 1105 42-43 0.13055799733927387 1105 44-45 0.14201818014872458 1105 46-47 0.14587138414274037 1105 48-49 0.10374505187136052 1105 50-51 0.16792993030459513 1105 52-53 0.01116937828068032 1105 54-55 -0.09903786807886306 1105 56-57 -0.1268358662024518 1105 58-59 -0.31046893494454864 1105 60-61 -0.25221864430617913 1105 62-63 -0.4100179431655313 1105 64-65 -0.03805806120553612 1105 66-67 -0.23093688214296648 1105 68-69 -0.26855446714685627 1105 70-71 -0.3719157159038673 1105 72-73 -0.20292654231949214 1105 74-75 -0.2622619038526004 1105 76-77 -0.0680102939963163 1105 78-79 -0.34409303001516633 1105 80-81 -0.33432193805683497 1105 82-83 -0.6441127562471003 1105 84-85 -0.8560837417206777 1105 86-87 -0.4081071590392824 1105 88-89 -0.9175719359169001 1105 90-91 -3.786404662500228 1105 92-93 -8.181769659148248 1105 94-95 -5.869163537966257 1105 96-97 -3.6130757255553263 1105 98-99 -2.654709759221001 1105 100-101 -2.0855147780699284 1106 1 1.551723572353513 1106 2 1.1426079813818468 1106 3 0.6978381901205779 1106 4 0.7914773359558538 1106 5 0.7703419918335257 1106 6 0.6395857358223083 1106 7 0.6647937408631819 1106 8 -0.35963729704806013 1106 9 -0.6301254929192979 1106 10-11 -0.6712152816439314 1106 12-13 -0.3789352916762567 1106 14-15 0.4594135062012228 1106 16-17 0.7515823477217936 1106 18-19 0.8115269388773285 1106 20-21 0.596410464373669 1106 22-23 0.668987012634247 1106 24-25 0.7760855450006332 1106 26-27 0.6927920882547269 1106 28-29 0.5905347554907863 1106 30-31 0.7550973250696202 1106 32-33 0.8495109837272707 1106 34-35 0.9490911283222303 1106 36-37 0.931333477630865 1106 38-39 0.8157819502326404 1106 40-41 0.7370685690162233 1106 42-43 1.0063737628254827 1106 44-45 1.0634962066023093 1106 46-47 0.9353893431047631 1106 48-49 0.517201182355052 1106 50-51 0.38870661662397765 1106 52-53 0.5491937470018335 1106 54-55 0.6158088213435811 1106 56-57 0.6224186720233718 1106 58-59 0.44559682084459595 1106 60-61 0.37788831430675174 1106 62-63 0.5330314439800397 1106 64-65 0.5417659855730967 1106 66-67 0.34711672322533005 1106 68-69 0.3140395458860006 1106 70-71 0.16591277212995692 1106 72-73 0.4083264152849466 1106 74-75 0.3731912884207773 1106 76-77 0.17840082271754198 1106 78-79 0.21466899434385667 1106 80-81 -0.015211449712708713 1106 82-83 -0.2603124284863476 1106 84-85 -0.037884777367086286 1106 86-87 0.24368994929631782 1106 88-89 -0.21292292113857414 1106 90-91 -3.42838155439736 1106 92-93 -8.57672524659637 1106 94-95 -6.052454042601454 1106 96-97 -3.3367217730321492 1106 98-99 -2.3826423937192747 1106 100-101 -1.9801809081657353 1107 1 1.824670846886086 1107 2 1.0899159316735911 1107 3 0.8686955349265268 1107 4 0.8577853030766605 1107 5 0.6675784185898195 1107 6 0.6786979304047946 1107 7 0.7252435097039296 1107 8 0.739443114639009 1107 9 0.6830100748333265 1107 10-11 0.6069416710447513 1107 12-13 0.47393995444884496 1107 14-15 0.7946106587230517 1107 16-17 0.8101026640120921 1107 18-19 0.8877062357589054 1107 20-21 0.5227263171145253 1107 22-23 0.5542714358901009 1107 24-25 0.8161331953022355 1107 26-27 0.7130746002093744 1107 28-29 0.7237454770490572 1107 30-31 0.8061241385819216 1107 32-33 1.0207967485001888 1107 34-35 0.7843013834749328 1107 36-37 0.5568072759753662 1107 38-39 0.6885204241247536 1107 40-41 0.8523188292657267 1107 42-43 0.8375121049700383 1107 44-45 0.5938955122830833 1107 46-47 0.4010532377836533 1107 48-49 0.3121708539718675 1107 50-51 0.4171970252577708 1107 52-53 0.6337362488109584 1107 54-55 0.6664918410552296 1107 56-57 0.5547712313638655 1107 58-59 0.5053365232586096 1107 60-61 0.41200996922913546 1107 62-63 0.2424626936344474 1107 64-65 0.27350175960199863 1107 66-67 0.36786011120504725 1107 68-69 0.39144568176388717 1107 70-71 0.6197970410392166 1107 72-73 0.5208802983517771 1107 74-75 0.11743803139524545 1107 76-77 0.14864474802533323 1107 78-79 -0.004019203912168479 1107 80-81 -0.08346858343407249 1107 82-83 -0.1760672001279282 1107 84-85 -0.11988224339039633 1107 86-87 -0.07233805427144802 1107 88-89 -0.30030436637077784 1107 90-91 -3.388009704137593 1107 92-93 -4.296845959090991 1107 94-95 -2.580521440611296 1107 96-97 -1.2461833150074497 1107 98-99 -0.9896401602616471 1107 100-101 -0.6994903394153891 1108 1 1.6146445217992387 1108 2 0.8841698597113492 1108 3 -0.5667659808202252 1108 4 -0.3009158951978037 1108 5 0.3985173954078931 1108 6 0.6007480853247458 1108 7 0.6049259643429963 1108 8 0.5106601366269743 1108 9 0.8711374994898406 1108 10-11 0.6563827967819265 1108 12-13 0.6128369317113425 1108 14-15 0.6969484004293491 1108 16-17 0.6791534558771843 1108 18-19 0.7006552689565524 1108 20-21 0.35947640783753343 1108 22-23 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0.922970560202014 2311 86-87 1.3115193649496142 2311 88-89 1.7085287105387401 2311 90-91 2.4440817633355323 2311 92-93 3.2849772805872703 2311 94-95 2.7659951446894944 2311 96-97 1.997225548574864 2311 98-99 1.854850056794529 2311 100-101 1.9812135999811211 2312 1 1.1523424936620899 2312 2 0.2585483628394698 2312 3 -0.320401041911758 2312 4 -0.6663223356369201 2312 5 -0.33812080973272174 2312 6 -0.5489831665396991 2312 7 -0.21661335778529178 2312 8 -0.04276221810401637 2312 9 -0.1765066979211909 2312 10-11 -0.2525844871510756 2312 12-13 -0.16346225896185018 2312 14-15 0.03257884660794019 2312 16-17 -0.3850413733846878 2312 18-19 -0.6558470664277181 2312 20-21 -0.10763778622629872 2312 22-23 0.17131112530319115 2312 24-25 0.06296188704938999 2312 26-27 0.12858847577525268 2312 28-29 -0.016342849662684955 2312 30-31 0.25038594449617335 2312 32-33 0.3185712338219986 2312 34-35 0.10735183313466479 2312 36-37 0.27084970852640566 2312 38-39 0.28931359100565857 2312 40-41 0.23567915383370774 2312 42-43 0.4138513427168604 2312 44-45 0.19748003886201104 2312 46-47 0.18965232150567246 2312 48-49 0.12260727051976161 2312 50-51 0.022564138412533907 2312 52-53 -0.141478224704624 2312 54-55 -0.25949748154893015 2312 56-57 -0.13721250140090646 2312 58-59 0.02284198946907523 2312 60-61 -0.03845626065850993 2312 62-63 -0.13940761121763146 2312 64-65 -0.17286076698469088 2312 66-67 0.1352814320796938 2312 68-69 0.09569294995319311 2312 70-71 0.10838466805012814 2312 72-73 0.015905043553228637 2312 74-75 0.13493184671306224 2312 76-77 0.14986097176389634 2312 78-79 -0.03241954929641011 2312 80-81 0.2278604209453725 2312 82-83 0.45217967710920703 2312 84-85 0.6644519663489703 2312 86-87 0.9915237247162985 2312 88-89 1.2637664814128975 2312 90-91 2.079203389394479 2312 92-93 2.9897077456986914 2312 94-95 2.6806456163602768 2312 96-97 2.133020553606727 2312 98-99 1.799920445740117 2312 100-101 1.8582553682015774 2313 1 -7.241576488142556 2313 2 -2.7941995546997376 2313 3 -1.734002186683501 2313 4 -0.8625564554191385 2313 5 -0.5004945823605809 2313 6 -0.1551921571860717 2313 7 -0.08547417029402027 2313 8 -0.002228689321846389 2313 9 0.03995055928017166 2313 10-11 -0.07725408555025126 2313 12-13 -0.29978374902653115 2313 14-15 -0.43257076482116474 2313 16-17 -0.5850512621649955 2313 18-19 -0.5939706412799595 2313 20-21 -0.5349308198714269 2313 22-23 -1.0469191320377789 2313 24-25 -0.20794241282496984 2313 26-27 -0.3286710523554177 2313 28-29 -0.5928633950580036 2313 30-31 -0.14688824229707365 2313 32-33 -0.3707768047093367 2313 34-35 -0.20112925021030748 2313 36-37 -0.1528781289718424 2313 38-39 -0.09257947056072169 2313 40-41 -0.10712704934001493 2313 42-43 -0.2682773044979996 2313 44-45 -0.3191435825256832 2313 46-47 -1.390076260559571 2313 48-49 -0.7162887925822936 2313 50-51 -0.4855382628160001 2313 52-53 -0.9300561017580016 2313 54-55 -0.7274188599285658 2313 56-57 -0.45747286716943947 2313 58-59 -0.5867286047884512 2313 60-61 -0.5642904083619875 2313 62-63 -0.3876020362723125 2313 64-65 -0.34806203274856884 2313 66-67 -0.38539019061098756 2313 68-69 -0.30542506391436675 2313 70-71 -0.1825240526907166 2313 72-73 -0.3150293880164128 2313 74-75 -0.15163802624519462 2313 76-77 0.006504487050786878 2313 78-79 -0.17438985968777132 2313 80-81 0.14989204177463122 2313 82-83 0.38364228588578797 2313 84-85 0.46714287797817633 2313 86-87 0.9022577048922003 2313 88-89 1.0542482395216943 2313 90-91 1.9600528937920814 2313 92-93 2.8257831796416397 2313 94-95 2.3568828772844697 2313 96-97 1.382618603771931 2313 98-99 1.3458177652989605 2313 100-101 1.2622300761917096 2314 1 0.6050117984560366 2314 2 -0.06038691046266109 2314 3 -1.2469815940668951 2314 4 -0.177212293209827 2314 5 -0.13098716886423034 2314 6 -3.4114195327080274 2314 7 -2.802745957328696 2314 8 -2.653248093309159 2314 9 -3.2925611533494035 2314 10-11 -2.523328328570912 2314 12-13 -1.326742382411581 2314 14-15 -1.2123972558176845 2314 16-17 -2.0058984885680005 2314 18-19 -3.2177363771109633 2314 20-21 -2.8223811328514117 2314 22-23 -4.102927884671672 2314 24-25 -3.22214296314489 2314 26-27 -1.8537542178141635 2314 28-29 -1.98382672507244 2314 30-31 -2.264477833300539 2314 32-33 -2.457308631416449 2314 34-35 -1.826905525478324 2314 36-37 -2.16791438602451 2314 38-39 -3.651486085977396 2314 40-41 -2.9106052372206257 2314 42-43 -1.7721800028408659 2314 44-45 -2.1726969280540445 2314 46-47 -1.6770215025691257 2314 48-49 -2.3451424792627016 2314 50-51 -3.1545685844405646 2314 52-53 -2.1802432722799914 2314 54-55 -2.7580668751491935 2314 56-57 -1.8450051878523617 2314 58-59 -1.262085675974994 2314 60-61 -1.5419738933613338 2314 62-63 -1.256597721702338 2314 64-65 -1.6667026730647123 2314 66-67 -1.3817651607022938 2314 68-69 -1.4353976251017606 2314 70-71 -1.371108676921775 2314 72-73 -2.304506931412959 2314 74-75 -2.5311279962207287 2314 76-77 -1.6300891831123003 2314 78-79 -2.5529899013669812 2314 80-81 -1.6880228207225692 2314 82-83 -1.2467599956649842 2314 84-85 -2.46639595701075 2314 86-87 -1.1430828103863142 2314 88-89 -0.021495715063402088 2314 90-91 1.1098458484620792 2314 92-93 2.2308176807646625 2314 94-95 2.2546185963666012 2314 96-97 1.3714944035882297 2314 98-99 1.0408429850796033 2314 100-101 1.0287638903796203 2315 1 -2.921532426375233 2315 2 -2.0206527845485525 2315 3 -2.8184815302035915 2315 4 -4.147375357513081 2315 5 -4.158563140853584 2315 6 -3.3415997083219757 2315 7 -5.754497535157789 2315 8 -3.3166053567969875 2315 9 -2.8744604682887527 2315 10-11 -4.3411739658962745 2315 12-13 -3.4086031766277927 2315 14-15 -2.3831973312372057 2315 16-17 -2.7642765431092897 2315 18-19 -2.494869056673288 2315 20-21 -1.8148321861904506 2315 22-23 -1.22989269497738 2315 24-25 -2.672166481060067 2315 26-27 -4.27597479558446 2315 28-29 -3.648627806085571 2315 30-31 -3.640135473277745 2315 32-33 -4.445006734372207 2315 34-35 -4.173831166290828 2315 36-37 -3.24919250361814 2315 38-39 -2.1308889539466023 2315 40-41 -1.823101461176055 2315 42-43 -3.448020008031026 2315 44-45 -6.960482817710417 2315 46-47 -3.874534381668795 2315 48-49 -2.9210476655712156 2315 50-51 -3.7157983889377597 2315 52-53 -3.537942753872155 2315 54-55 -2.353450673333043 2315 56-57 -1.8681184326961606 2315 58-59 -3.140943779822809 2315 60-61 -3.5499521433446084 2315 62-63 -5.20168967472328 2315 64-65 -4.769373375621882 2315 66-67 -4.118940801007945 2315 68-69 -2.729588294571652 2315 70-71 -3.935803420464989 2315 72-73 -3.006014916353383 2315 74-75 -1.9908004484296882 2315 76-77 -3.818842145660021 2315 78-79 -2.4569025931342914 2315 80-81 -1.9805459483619998 2315 82-83 -1.5307081062235177 2315 84-85 -2.9494611320468884 2315 86-87 -2.265582257918048 2315 88-89 -1.9900085718615976 2315 90-91 -0.9137303546516122 2315 92-93 -0.2516179845591857 2315 94-95 -0.2975040982188055 2315 96-97 -0.6621071218324062 2315 98-99 -0.9908737619061494 2315 100-101 -1.1535401962636218 2316 1 -7.49590524481685 2316 2 -6.57734219390645 2316 3 -3.676088380428734 2316 4 -4.3977776606060175 2316 5 -2.296337450400241 2316 6 -2.923250890977073 2316 7 -3.328761737036963 2316 8 -2.6663591241044564 2316 9 -7.297997571917389 2316 10-11 -7.009881850615088 2316 12-13 -6.252272307378938 2316 14-15 -4.903890368177382 2316 16-17 -4.605198096642493 2316 18-19 -4.197393600852809 2316 20-21 -2.869065068536564 2316 22-23 -3.514539269337547 2316 24-25 -3.0725972564557793 2316 26-27 -4.123560449761978 2316 28-29 -4.209873311152585 2316 30-31 -6.737487021854662 2316 32-33 -4.949380923702474 2316 34-35 -5.51852055455813 2316 36-37 -4.534104292206017 2316 38-39 -6.676789996876508 2316 40-41 -4.092681969248481 2316 42-43 -5.038347861324851 2316 44-45 -3.3243113933571067 2316 46-47 -4.184422179705262 2316 48-49 -5.416126702574665 2316 50-51 -4.807935287987629 2316 52-53 -6.461024827921232 2316 54-55 -5.169686213094085 2316 56-57 -4.549482783264022 2316 58-59 -3.1162957291210205 2316 60-61 -3.249912071214773 2316 62-63 -4.441644751756673 2316 64-65 -3.010380873328277 2316 66-67 -4.045163264600308 2316 68-69 -3.104223690916651 2316 70-71 -2.4196617338501376 2316 72-73 -1.9158684421735437 2316 74-75 -2.333865333644127 2316 76-77 -2.976552339798946 2316 78-79 -4.872330363120266 2316 80-81 -5.686273099339022 2316 82-83 -3.2101410855863683 2316 84-85 -1.973112652151496 2316 86-87 -2.053911250103983 2316 88-89 -0.9983356658940181 2316 90-91 -0.9295161374817589 2316 92-93 -1.0575692231043572 2316 94-95 -1.8557315391915044 2316 96-97 -3.4650835320022892 2316 98-99 -4.883749359033612 2316 100-101 -4.384060923045514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17558.0 3 5937.0 4 5666.0 5 6520.0 6 7201.0 7 8521.0 8 10081.0 9 12044.0 10 14218.0 11 16981.0 12 20117.0 13 22986.0 14 25525.0 15 28466.0 16 32588.0 17 35211.0 18 31034.0 19 29360.0 20 30801.0 21 33837.0 22 37252.0 23 41547.0 24 46318.0 25 52509.0 26 58771.0 27 66533.0 28 75035.0 29 85646.0 30 96439.0 31 108933.0 32 121046.0 33 134106.0 34 152008.0 35 176446.0 36 187526.0 37 150270.0 38 43556.0 39 702.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.286260606105465 20.022600040956405 13.985379056472963 21.705760296465165 2 15.430656062774725 20.577184857137077 41.15811946202961 22.83403961805859 3 19.142296059091425 26.16631058523997 29.72566105324445 24.965732302424147 4 12.428713052549405 17.139511461426217 37.71180063291607 32.719974853108305 5 13.341619123696258 38.11703687110925 33.74834650129295 14.792997503901542 6 30.470995325774563 36.83918335280927 18.20144964153744 14.488371679878723 7 28.012036298443338 33.65434736251846 20.816221360480817 17.517394978557384 8 26.302242710204727 35.0818312834383 19.794582749294218 18.82134325706276 9 27.356347597056562 16.78486740917267 20.23439088774944 35.62439410602133 10-11 24.89021703882889 27.238513693934436 27.122153873338323 20.74911539389835 12-13 26.10813868038553 24.849509898515112 27.717076141550766 21.3252752795486 14-15 23.067902514140187 25.716288114345502 26.66918376815028 24.546625603364035 16-17 21.64847763397971 29.252189173836417 27.00342164058116 22.09591155160271 18-19 21.551369895976606 27.228862080462957 30.275055239830824 20.944712783729617 20-21 22.784343371892675 26.27297289492721 30.199050911511456 20.743632821668655 22-23 22.31141354907139 26.422373013255168 30.02006743130954 21.246146006363904 24-25 21.834208765184236 26.609349957895308 30.358124476778215 21.19831680014224 26-27 21.599842131403875 26.878857339593083 30.235985395672987 21.28531513333006 28-29 21.351824859340294 26.92728864986933 30.493606781104305 21.22727970968607 30-31 21.527677627744797 26.78741468040341 30.38155516263817 21.303352529213626 32-33 21.435708186132562 26.854087633456132 30.179396847676898 21.530807332734405 34-35 21.891371817060108 26.87765149822227 29.998125848646108 21.232850836071513 36-37 21.811321723854288 26.698854947034295 29.9637708985316 21.52605243057982 38-39 21.45504062972995 27.01359756443843 30.06521003755565 21.46615176827597 40-41 21.645515390285187 26.74868727923265 30.14066144088775 21.46513588959441 42-43 21.54861288233419 26.76336248413306 29.904577405582938 21.783447227949807 44-45 21.54852204106392 27.055584647210395 29.655835436203237 21.740057875522446 46-47 21.670864446288174 27.20763699577259 29.178642684998618 21.94285587294062 48-49 21.807728989602346 27.269134309393262 29.017070550552244 21.90606615045215 50-51 21.687219305853457 27.479884383839526 28.816915471306793 22.015980839000225 52-53 21.80017425041321 27.69341511789076 28.556120169053038 21.950290462643 54-55 21.474690235756775 27.54201825978697 28.65944212793708 22.323849376519178 56-57 21.5516594175912 27.759596093303156 28.67149290782594 22.017251581279705 58-59 21.639345962671104 27.305705329556172 28.733377473205522 22.3215712345672 60-61 21.60434237294831 27.478248632206835 28.831129392245554 22.0862796025993 62-63 21.47498707619932 27.411492332372013 28.80027630024297 22.313244291185697 64-65 21.386314703989378 27.1592890007631 28.826605368464424 22.627790926783096 66-67 21.385172644767277 27.506208782486084 28.487244559738745 22.621374013007888 68-69 21.440935552388176 27.24335131630568 28.69397994673498 22.62173318457117 70-71 21.396493938045648 27.413386719157018 28.509605151982193 22.680514190815142 72-73 21.576600640723086 27.26651902027804 28.305106890502348 22.85177344849653 74-75 21.472163075417587 27.438730263484505 28.175891139472487 22.913215521625418 76-77 21.565619587116487 27.320086003401006 28.277496336654306 22.836798072828202 78-79 21.679154101833443 27.401702823085834 28.130249070235276 22.788894004845446 80-81 21.51261006601614 27.654542049776197 27.930642784051972 22.90220510015569 82-83 21.770044559798745 27.51503958057413 27.789977763333784 22.92493809629334 84-85 21.74513114516019 27.13922855090748 27.861690240753052 23.25395006317928 86-87 21.555032585977532 27.04238474127579 28.264537969715516 23.138044703031166 88-89 21.84266452467685 26.949057593640426 28.028252924734943 23.180024956947783 90-91 21.70327814454005 27.093508171668002 28.160314994064912 23.042898689727036 92-93 22.08930931352129 26.630133238637843 28.147061833661226 23.13349561417964 94-95 21.76829310499976 26.598140554095895 28.201818705013203 23.431747635891142 96-97 22.172204411664783 26.660810802463413 27.596241869429054 23.570742916442747 98-99 22.150675299092804 26.593202891261335 27.414758087338438 23.841363722307424 100-101 22.350101799361813 26.58599845941325 27.540509619767768 23.52339012145717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3218.0 1 1982.5 2 754.5 3 760.0 4 702.0 5 656.0 6 918.0 7 1715.5 8 2794.0 9 3323.5 10 3067.5 11 2731.5 12 2679.5 13 2799.0 14 2998.5 15 3235.0 16 3456.0 17 3709.5 18 4056.0 19 4458.0 20 4891.5 21 5515.0 22 6339.5 23 7358.5 24 8646.5 25 10556.0 26 13059.5 27 15884.0 28 18562.0 29 21556.0 30 25088.5 31 28361.5 32 32403.5 33 37026.5 34 41626.5 35 45709.0 36 50175.0 37 55082.0 38 58057.5 39 59349.5 40 60943.0 41 63893.5 42 67219.5 43 70320.0 44 72857.5 45 74453.5 46 74779.0 47 75005.5 48 76997.5 49 77937.5 50 75768.0 51 72214.0 52 69434.0 53 67184.0 54 63863.5 55 58646.5 56 51866.0 57 45767.5 58 40962.0 59 35993.0 60 30633.5 61 24961.5 62 19569.0 63 15309.5 64 11856.0 65 9201.0 66 6992.0 67 5251.5 68 3889.5 69 2796.5 70 2145.5 71 1666.0 72 1338.0 73 1081.5 74 824.5 75 644.0 76 450.0 77 301.5 78 209.0 79 136.0 80 80.0 81 57.0 82 44.0 83 26.0 84 14.5 85 11.0 86 9.5 87 7.5 88 6.0 89 6.0 90 5.5 91 3.0 92 2.0 93 0.5 94 0.5 95 1.0 96 1.5 97 2.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13551504340177253 2 0.12280126842080626 3 0.12836970474967907 4 0.13807750967700605 5 0.12477239632483202 6 0.14108347973064536 7 0.16867927038700634 8 0.15645827738204646 9 0.16572257853096764 10-11 0.1602526985972961 12-13 0.15187540500518654 14-15 0.1554727134300336 16-17 0.15148117942438138 18-19 0.16540227024656348 20-21 0.1703300900066279 22-23 0.20317400870745753 24-25 0.22569414501095209 26-27 0.23784122071951094 28-29 0.2329873182558475 30-31 0.22446219007093596 32-33 0.22500425024454307 34-35 0.21576458819442224 36-37 0.22379693440332726 38-39 0.2118962496827716 40-41 0.21670087394883444 42-43 0.2094569789015397 44-45 0.20137535449503396 46-47 0.2175632424068457 48-49 0.2531174619757108 50-51 0.2554335372629411 52-53 0.2560741538317494 54-55 0.2550146725833356 56-57 0.23007990459740943 58-59 0.23015382189381042 60-61 0.20376534707866525 62-63 0.19524021889375376 64-65 0.19733454229178116 66-67 0.20398709896786815 68-69 0.2039378207702675 70-71 0.21130491131156384 72-73 0.2396645140307348 74-75 0.24178347652756252 76-77 0.2557045673497446 78-79 0.2526493190985047 80-81 0.26590515425307804 82-83 0.26538773317827125 84-85 0.2413153336503564 86-87 0.2443213037039957 88-89 0.2468344917816286 90-91 0.42800578526039834 92-93 0.5716024530686765 94-95 0.33297278118755524 96-97 0.2800233578656627 98-99 0.3105758403780623 100-101 0.32962186375071145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2029295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.14132295148323 #Duplication Level Percentage of deduplicated Percentage of total 1 78.95271691830752 45.904154122443515 2 11.784097651763794 13.702860545260275 3 3.9606335738187606 6.908294271236512 4 1.7764947382046616 4.131510171822715 5 0.9440356296465116 2.7443740210492322 6 0.5883062545321524 2.052294236347882 7 0.3599231388810909 1.464848521877794 8 0.2495273073363257 1.1606278208844256 9 0.18938764207413447 0.9910123254766948 >10 1.0640383787378833 11.323554839632985 >50 0.08565737578606394 3.433988789849348 >100 0.043187762956984545 4.185414494544229 >500 0.0013001921440439297 0.47770931671147476 >1k 4.3339738134797476E-4 0.3383627336639763 >5k 1.733589525391899E-4 0.6513759804299811 >10k+ 8.667947626959494E-5 0.5296178087689494 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10509 0.5178645785851737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7569 0.3729866776392787 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5356 0.2639340263490522 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.927819760064456E-5 0.0 4 0.0 0.0 0.0 4.927819760064456E-5 0.0 5 0.0 0.0 0.0 4.927819760064456E-5 0.0 6 0.0 0.0 0.0 4.927819760064456E-5 0.0 7 0.0 0.0 0.0 4.927819760064456E-5 0.0 8 0.0 0.0 0.0 4.927819760064456E-5 0.0 9 0.0 0.0 0.0 4.927819760064456E-5 0.0 10-11 0.0 0.0 0.0 4.927819760064456E-5 0.0 12-13 0.0 0.0 0.0 4.927819760064456E-5 4.927819760064456E-5 14-15 0.0 0.0 0.0 4.927819760064456E-5 4.927819760064456E-5 16-17 0.0 0.0 0.0 4.927819760064456E-5 4.927819760064456E-5 18-19 0.0 0.0 0.0 9.855639520128912E-5 4.927819760064456E-5 20-21 0.0 0.0 0.0 1.7247369160225596E-4 4.927819760064456E-5 22-23 0.0 0.0 0.0 5.174210748067679E-4 4.927819760064456E-5 24-25 0.0 0.0 0.0 9.362857544122466E-4 4.927819760064456E-5 26-27 2.463909880032228E-5 0.0 0.0 0.002316075287230294 4.927819760064456E-5 28-29 4.927819760064456E-5 0.0 0.0 0.008697601876513765 4.927819760064456E-5 30-31 4.927819760064456E-5 0.0 0.0 0.029443723066385125 4.927819760064456E-5 32-33 4.927819760064456E-5 0.0 0.0 0.0715519429161359 4.927819760064456E-5 34-35 4.927819760064456E-5 0.0 0.0 0.13100608832131355 4.927819760064456E-5 36-37 4.927819760064456E-5 0.0 0.0 0.23601792740828711 4.927819760064456E-5 38-39 7.391729640096684E-5 0.0 0.0 0.39868525768801477 4.927819760064456E-5 40-41 9.855639520128912E-5 0.0 0.0 0.5816305662804078 4.927819760064456E-5 42-43 9.855639520128912E-5 0.0 0.0 0.7595002205199342 4.927819760064456E-5 44-45 9.855639520128912E-5 0.0 0.0 0.9690064776190745 4.927819760064456E-5 46-47 9.855639520128912E-5 0.0 0.0 1.216900450649117 4.927819760064456E-5 48-49 9.855639520128912E-5 0.0 0.0 1.483101274087799 4.927819760064456E-5 50-51 9.855639520128912E-5 0.0 0.0 1.7770210836768434 4.927819760064456E-5 52-53 9.855639520128912E-5 0.0 0.0 2.060789584560155 4.927819760064456E-5 54-55 9.855639520128912E-5 0.0 0.0 2.3430058222190464 4.927819760064456E-5 56-57 9.855639520128912E-5 0.0 0.0 2.669399964026916 4.927819760064456E-5 58-59 9.855639520128912E-5 0.0 0.0 3.0365471752505178 4.927819760064456E-5 60-61 1.7247369160225596E-4 0.0 0.0 3.432078628292092 4.927819760064456E-5 62-63 1.9711279040257823E-4 0.0 0.0 3.8221402013999937 4.927819760064456E-5 64-65 1.9711279040257823E-4 0.0 0.0 4.194092036889659 4.927819760064456E-5 66-67 2.463909880032228E-4 0.0 0.0 4.6006864452925775 4.927819760064456E-5 68-69 2.956691856038674E-4 0.0 0.0 5.060599863499393 4.927819760064456E-5 70-71 2.956691856038674E-4 0.0 0.0 5.561685215801547 4.927819760064456E-5 72-73 2.956691856038674E-4 0.0 0.0 6.050401740505939 4.927819760064456E-5 74-75 3.2030828440418965E-4 0.0 0.0 6.523546354768528 4.927819760064456E-5 76-77 3.449473832045119E-4 0.0 0.0 7.014800706649353 4.927819760064456E-5 78-79 3.9422558080515647E-4 0.0 0.0 7.547793691897925 4.927819760064456E-5 80-81 3.9422558080515647E-4 0.0 0.0 8.10096609906396 4.927819760064456E-5 82-83 3.9422558080515647E-4 0.0 0.0 8.615282647421889 4.927819760064456E-5 84-85 3.9422558080515647E-4 0.0 0.0 9.096508886090982 4.927819760064456E-5 86-87 3.9422558080515647E-4 0.0 0.0 9.58416592954696 4.927819760064456E-5 88-89 3.9422558080515647E-4 0.0 0.0 10.106416267718592 4.927819760064456E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5500 0.0 49.337177 1 GTATCAA 10305 0.0 43.043205 1 TATCAAC 13625 0.0 33.63424 2 ATCAACG 13655 0.0 33.63315 3 TCAACGC 13770 0.0 33.5951 4 CAACGCA 14020 0.0 32.826027 5 AACGCAG 14530 0.0 31.771015 6 ACGCAGA 16235 0.0 28.229856 7 CGCAGAG 16395 0.0 27.83861 8 GTACATG 11940 0.0 27.613499 1 TACATGG 12100 0.0 27.248363 2 GCAGAGT 16755 0.0 25.48547 9 ACATGGG 12380 0.0 25.33172 3 GAGTACT 8905 0.0 22.989847 12-13 AGTACTT 9260 0.0 22.441525 12-13 AGAGTAC 15585 0.0 21.84152 10-11 CATGGGG 4605 0.0 21.533863 4 CATGGGA 7565 0.0 21.449762 4 GTACTTT 9590 0.0 21.152172 14-15 CAGAGTA 16245 0.0 20.924942 10-11 >>END_MODULE