FastQCFastQC Report
Fri 27 May 2016
SRR939889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029295
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115770.570493693622662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93970.4630672228532569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65760.3240534274218386No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.1064901850149929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52700.055.1358031
GTATCAA97750.042.3683321
TATCAAC127500.032.1784862
ATCAACG127950.032.101383
TCAACGC129700.031.6311074
CAACGCA132300.031.0451265
AACGCAG136250.030.0934286
TGGTATC25300.026.4681952
ACGCAGA155900.026.209697
CGCAGAG155450.026.1946098
GTACATG113700.025.6384411
TACATGG113450.025.1897962
GTGGTAT28500.024.6606791
GCAGAGT164500.024.2951099
ACATGGG114350.024.2898563
GAGTACT92450.022.81261412-13
CATGGGA69150.022.299274
GTACTTT98000.021.40044614-15
AGTACTT95450.020.76246612-13
CAGAGTA162600.020.62259310-11
Produced by FastQC (version 0.11.5)