##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939889_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2029295 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40052185611259 31.0 31.0 34.0 30.0 34.0 2 31.611918424871693 31.0 31.0 34.0 30.0 34.0 3 31.65771166833802 31.0 31.0 34.0 30.0 34.0 4 35.473259925244975 37.0 35.0 37.0 33.0 37.0 5 35.20771844409019 37.0 35.0 37.0 32.0 37.0 6 35.241627757423146 37.0 35.0 37.0 32.0 37.0 7 35.16041383830345 37.0 35.0 37.0 32.0 37.0 8 35.109174861220275 37.0 35.0 37.0 32.0 37.0 9 36.693950362071554 39.0 35.0 39.0 32.0 39.0 10-11 36.547772255881966 38.5 35.0 39.0 32.0 39.0 12-13 36.48650689032398 38.0 35.0 39.0 32.0 39.0 14-15 37.48538112989979 39.0 36.0 41.0 32.0 41.0 16-17 37.42165505754461 39.0 36.0 41.0 32.0 41.0 18-19 37.43377305911659 39.0 36.0 41.0 32.0 41.0 20-21 37.38674933905618 39.0 36.0 41.0 32.0 41.0 22-23 37.23825909983516 39.0 36.0 40.0 31.0 41.0 24-25 37.07871822480221 39.0 36.0 40.0 31.0 41.0 26-27 36.78218272848453 39.0 35.5 40.0 30.5 41.0 28-29 36.54962536250274 38.0 35.0 40.0 30.0 41.0 30-31 36.27348660495394 38.0 35.0 40.0 30.0 41.0 32-33 36.19345018836591 38.0 35.0 40.0 30.0 41.0 34-35 36.238974865655315 38.0 35.0 40.0 30.0 41.0 36-37 36.18641794317731 38.0 35.0 40.0 30.0 41.0 38-39 36.04654104011492 38.0 35.0 40.0 29.5 41.0 40-41 35.86050894522482 38.0 35.0 40.0 29.0 41.0 42-43 35.6244382901451 38.0 34.5 40.0 28.5 41.0 44-45 35.31799294828993 38.0 34.0 40.0 27.0 41.0 46-47 34.94161494509177 38.0 34.0 40.0 26.0 41.0 48-49 34.58121761498451 38.0 33.0 40.0 24.5 41.0 50-51 34.302671617482915 38.0 33.0 40.0 24.0 41.0 52-53 34.01419409203689 38.0 33.0 40.0 23.5 41.0 54-55 33.70337136788885 37.0 33.0 40.0 22.5 41.0 56-57 32.310453876839 36.5 30.5 39.5 14.5 41.0 58-59 28.85022286064865 34.0 23.0 38.5 7.0 40.0 60-61 28.853125839269303 34.0 23.0 38.0 6.5 40.0 62-63 30.821106837596307 35.0 29.0 38.0 4.5 40.0 64-65 30.40428695680027 35.0 29.0 38.0 2.0 40.0 66-67 24.940052826227827 28.0 17.5 36.0 2.0 39.0 68-69 17.349776646569374 13.0 7.0 29.0 2.0 35.5 70-71 16.203328988638912 13.0 7.5 25.5 2.0 33.5 72-73 18.271228185157902 17.0 7.0 30.0 2.0 34.5 74-75 18.954913405887268 17.5 7.5 30.5 2.0 35.0 76-77 19.92540291086313 22.5 7.5 31.0 2.0 34.5 78-79 22.920303849366405 27.5 10.5 34.0 2.0 36.0 80-81 25.543547636001666 31.5 20.0 34.5 2.0 36.0 82-83 26.56762126748452 32.5 25.0 35.0 2.0 36.0 84-85 26.56907004649398 33.0 25.0 35.0 2.0 36.0 86-87 26.272906107786202 33.0 24.5 35.0 2.0 35.5 88-89 25.825475596204594 33.0 23.0 35.0 2.0 35.0 90-91 24.14312507545724 31.5 10.0 34.5 2.0 35.0 92-93 21.969286131390458 30.5 2.0 34.0 2.0 35.0 94-95 17.606087582140596 20.0 2.0 33.0 2.0 35.0 96-97 16.288284601302422 13.5 2.0 32.5 2.0 34.5 98-99 15.215029603877209 2.0 2.0 31.5 2.0 34.0 100-101 14.601890311659961 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24378686261834304 1101 2 0.28369882836898697 1101 3 0.29447561105699194 1101 4 0.2664339480135851 1101 5 0.26167299779839936 1101 6 0.30662592433980507 1101 7 0.4314929817605915 1101 8 0.4748019090104023 1101 9 0.6662837383026385 1101 10-11 0.5638430477862642 1101 12-13 0.6302773848915777 1101 14-15 0.5656379748620708 1101 16-17 0.5576331975942708 1101 18-19 0.6276360970257926 1101 20-21 0.6474412546032298 1101 22-23 0.6521229764428043 1101 24-25 0.5729312018823407 1101 26-27 0.741887191857117 1101 28-29 0.745182625769182 1101 30-31 0.8809763977943845 1101 32-33 0.8472926692504785 1101 34-35 1.1876297099596087 1101 36-37 1.0753230253842432 1101 38-39 0.880223264994207 1101 40-41 0.8792993443095938 1101 42-43 0.927569332684385 1101 44-45 0.8464597294381662 1101 46-47 0.7146747486876635 1101 48-49 0.746160572798992 1101 50-51 0.7710206740649781 1101 52-53 0.8055862877279267 1101 54-55 0.7236698187855026 1101 56-57 1.5437473492306069 1101 58-59 -7.264736675036367 1101 60-61 -9.248394246943192 1101 62-63 -2.205644010454879 1101 64-65 0.31721728533474547 1101 66-67 -0.8142403507463243 1101 68-69 -3.4769184363452723 1101 70-71 -3.6370094702391604 1101 72-73 -6.486247104062894 1101 74-75 -7.2848046615261595 1101 76-77 -8.28602765817115 1101 78-79 -10.993376218009693 1101 80-81 -6.891016461844167 1101 82-83 -3.1609955201614177 1101 84-85 -2.122078450788024 1101 86-87 -1.807835247460801 1101 88-89 -2.221919193378053 1101 90-91 -8.181135070189931 1101 92-93 -13.756060411032092 1101 94-95 -11.41044814452907 1101 96-97 -11.642448189799234 1101 98-99 -11.460487314189109 1101 100-101 -11.316842066435338 1102 1 0.4430644309822753 1102 2 0.5839134083696891 1102 3 0.508748409951437 1102 4 0.38611165364967803 1102 5 0.39532654058248085 1102 6 0.5014961154322464 1102 7 0.4760026489309581 1102 8 0.44280771724636026 1102 9 0.7402815500663493 1102 10-11 0.726909779767908 1102 12-13 0.6940778033502468 1102 14-15 0.7653768071999991 1102 16-17 0.7556321422222965 1102 18-19 0.7985431634053626 1102 20-21 0.8695024956985264 1102 22-23 0.8244495827023428 1102 24-25 0.8768437351625309 1102 26-27 0.919207441899097 1102 28-29 0.7518677245442476 1102 30-31 0.6973571269235777 1102 32-33 0.7496894429857264 1102 34-35 0.5686291398373342 1102 36-37 0.7477912396837993 1102 38-39 0.8032030210409218 1102 40-41 0.6983733937829228 1102 42-43 0.6541355083816782 1102 44-45 0.658211674588344 1102 46-47 0.6358237480216005 1102 48-49 0.6373986791074131 1102 50-51 0.5996604158309538 1102 52-53 0.5990352783900974 1102 54-55 0.42062907441616204 1102 56-57 1.0340271335066689 1102 58-59 -8.800285828247965 1102 60-61 -9.826224470434816 1102 62-63 -2.7173714163616403 1102 64-65 0.262077097987774 1102 66-67 -1.1155022305999722 1102 68-69 -3.8040406196368135 1102 70-71 -4.400705781418909 1102 72-73 -6.692965090959309 1102 74-75 -7.697806585919459 1102 76-77 -8.411777212128888 1102 78-79 -11.581580268276396 1102 80-81 -7.8795351402675315 1102 82-83 -3.5977059515539374 1102 84-85 -2.629214896361642 1102 86-87 -2.3506497695267505 1102 88-89 -2.449412970927046 1102 90-91 -7.971649960713538 1102 92-93 -14.10986417826722 1102 94-95 -12.301869603267162 1102 96-97 -12.185859126183551 1102 98-99 -11.559115699636045 1102 100-101 -11.354110119846016 1103 1 0.3151864734995584 1103 2 0.32935865579809587 1103 3 0.28578008639860286 1103 4 0.20397677664318792 1103 5 0.24658701554694318 1103 6 0.2850079939618695 1103 7 0.3156391854744598 1103 8 0.3704889049999167 1103 9 0.32655704094138116 1103 10-11 0.36560965816056523 1103 12-13 0.3176687156040927 1103 14-15 0.3568189531442627 1103 16-17 0.41576476159575293 1103 18-19 0.49119166297405314 1103 20-21 0.5319253313695711 1103 22-23 0.44089703032890526 1103 24-25 0.4622828304304534 1103 26-27 0.32571379342493145 1103 28-29 0.5254303887721434 1103 30-31 0.40782908157652287 1103 32-33 0.44489061973830246 1103 34-35 0.43505325453922694 1103 36-37 0.12463108911601495 1103 38-39 0.22258279600659847 1103 40-41 0.24327740019795385 1103 42-43 0.42632346295950896 1103 44-45 0.37261764317808854 1103 46-47 0.376562509835793 1103 48-49 0.45734609304620477 1103 50-51 0.45419978800131844 1103 52-53 0.44468218303602924 1103 54-55 0.4161516380446315 1103 56-57 0.952637750346355 1103 58-59 -6.286603898258885 1103 60-61 -8.637750631475622 1103 62-63 -2.427896111708197 1103 64-65 -0.12945227719792385 1103 66-67 -1.6700519852314883 1103 68-69 -4.15444744091379 1103 70-71 -4.494514631472974 1103 72-73 -7.45581435308663 1103 74-75 -7.851058891875127 1103 76-77 -8.768202823451514 1103 78-79 -11.824307713207933 1103 80-81 -8.17627941030872 1103 82-83 -4.405846077225899 1103 84-85 -3.397491755733416 1103 86-87 -3.077240357052549 1103 88-89 -3.29513222144589 1103 90-91 -8.570909188678145 1103 92-93 -14.181674636498247 1103 94-95 -12.383274035473557 1103 96-97 -12.265804353178392 1103 98-99 -11.917441494639872 1103 100-101 -11.55475463321233 1104 1 0.07328318515060772 1104 2 0.1139465598416578 1104 3 0.1066965322898028 1104 4 0.14391070823086238 1104 5 0.04563300463151876 1104 6 0.09787938418690345 1104 7 0.1273143931408356 1104 8 0.2561851007200744 1104 9 0.11484763666359044 1104 10-11 0.12377462326627864 1104 12-13 0.08731924626888343 1104 14-15 0.13869605359774795 1104 16-17 0.16189790055815934 1104 18-19 0.22026388421401322 1104 20-21 0.24870051637021362 1104 22-23 0.26318401956651627 1104 24-25 0.32261960648666843 1104 26-27 0.2584646895135876 1104 28-29 0.3273247366173706 1104 30-31 0.15765384723772513 1104 32-33 0.18872806656524688 1104 34-35 0.42148735629464795 1104 36-37 0.40293012450602106 1104 38-39 0.27762734361547814 1104 40-41 0.49696722155793793 1104 42-43 0.2988067915290813 1104 44-45 0.19813292764241908 1104 46-47 0.1964508443886004 1104 48-49 0.3122320270807535 1104 50-51 0.12309214624670517 1104 52-53 0.054584571277978 1104 54-55 0.028074491607419816 1104 56-57 0.7699157979155231 1104 58-59 -6.373992546707704 1104 60-61 -8.503659265491045 1104 62-63 -2.376146715099022 1104 64-65 -0.17976345937338323 1104 66-67 -1.7709119554655288 1104 68-69 -4.216885025691919 1104 70-71 -4.678535612538349 1104 72-73 -7.759621428290988 1104 74-75 -8.489578579269644 1104 76-77 -8.875831533044245 1104 78-79 -12.276682292826052 1104 80-81 -8.54810256723832 1104 82-83 -4.683500431947724 1104 84-85 -3.6056546592586685 1104 86-87 -3.3466530488872728 1104 88-89 -3.5453192196053784 1104 90-91 -8.740177290344578 1104 92-93 -14.664805667057857 1104 94-95 -12.485363732626222 1104 96-97 -12.01855073941421 1104 98-99 -11.345034945383125 1104 100-101 -11.028416021068292 1105 1 0.14952411459325177 1105 2 0.20540715022338318 1105 3 0.1283740817424608 1105 4 0.126072729943715 1105 5 0.03253929970187386 1105 6 0.12298204576238447 1105 7 0.21149664968086057 1105 8 0.10400520148940018 1105 9 0.1582159154135354 1105 10-11 0.22373774323675377 1105 12-13 0.25060198782718857 1105 14-15 0.23442197119735653 1105 16-17 0.27571668522585924 1105 18-19 0.25136714076533906 1105 20-21 0.23887317704193123 1105 22-23 0.2049103956364391 1105 24-25 0.21001972209273845 1105 26-27 0.2887198465985037 1105 28-29 0.39234291068169824 1105 30-31 0.2798691343512232 1105 32-33 0.4246597591962029 1105 34-35 0.4100155959946079 1105 36-37 0.3394455195059649 1105 38-39 0.3740596773517453 1105 40-41 0.29336709992679744 1105 42-43 0.20645820911551738 1105 44-45 0.23993527610244314 1105 46-47 0.001955994883601875 1105 48-49 -0.0281866495122145 1105 50-51 0.08832701107797902 1105 52-53 -0.028062115685649758 1105 54-55 0.039330196597312295 1105 56-57 0.6127276754032138 1105 58-59 -6.531160428744329 1105 60-61 -8.850032878312401 1105 62-63 -2.785741218350676 1105 64-65 -0.47274622766795105 1105 66-67 -1.762353293859924 1105 68-69 -3.889499803310283 1105 70-71 -4.560679088326973 1105 72-73 -5.9713231533443665 1105 74-75 -6.4645193405751264 1105 76-77 -8.601150861851245 1105 78-79 -11.846152665476746 1105 80-81 -8.081957940096121 1105 82-83 -4.246318513376568 1105 84-85 -3.238597986868186 1105 86-87 -2.9885387834000134 1105 88-89 -3.0064555057887574 1105 90-91 -8.24857014479767 1105 92-93 -11.235759713646726 1105 94-95 -11.439119188140483 1105 96-97 -11.74130364651137 1105 98-99 -11.29762104271816 1105 100-101 -10.95241256418333 1106 1 0.08702799341874368 1106 2 0.05992404250839911 1106 3 0.11586389982554479 1106 4 0.01929642180981972 1106 5 -0.017446154702525973 1106 6 -0.03130551096543854 1106 7 0.08035404466166085 1106 8 0.09980200921602744 1106 9 -0.13451710502787506 1106 10-11 0.033882568883555564 1106 12-13 0.058979281182764964 1106 14-15 0.18072593535701031 1106 16-17 0.17151688692477762 1106 18-19 0.11956513541256442 1106 20-21 0.07942508542407012 1106 22-23 0.11720312089923368 1106 24-25 0.06796501125917587 1106 26-27 0.09103283872449452 1106 28-29 -0.019513931566471854 1106 30-31 0.0018746810716692153 1106 32-33 -0.005250349023029344 1106 34-35 0.035330992992392396 1106 36-37 -0.03880857265493631 1106 38-39 -0.11391911516988529 1106 40-41 0.022118409030397856 1106 42-43 -0.026681991031878738 1106 44-45 -0.022035021791353415 1106 46-47 -0.06627375764733046 1106 48-49 -0.1312853462268535 1106 50-51 -0.05162780060503991 1106 52-53 -0.10461825198757424 1106 54-55 -0.18822619182827083 1106 56-57 0.8223764962402669 1106 58-59 -6.574900731680604 1106 60-61 -9.115471088622321 1106 62-63 -2.680200800989052 1106 64-65 -0.2837611223578378 1106 66-67 -1.5971759345643193 1106 68-69 -4.115916059467917 1106 70-71 -4.773103936083073 1106 72-73 -6.196532609920915 1106 74-75 -6.749913865612946 1106 76-77 -8.45792038204053 1106 78-79 -12.034029638829214 1106 80-81 -8.057273821947096 1106 82-83 -4.317406475441654 1106 84-85 -3.3734228094119203 1106 86-87 -3.2192047777369304 1106 88-89 -3.3003727801380833 1106 90-91 -8.401196836935291 1106 92-93 -11.706509733040853 1106 94-95 -11.562185108779712 1106 96-97 -12.087196212742313 1106 98-99 -11.57955904938974 1106 100-101 -11.335318580241669 1107 1 0.11163761916433046 1107 2 0.13894108073682787 1107 3 0.1710110700179044 1107 4 0.006221679114233325 1107 5 0.017653509437373316 1107 6 0.10942996735271038 1107 7 0.2141153171314727 1107 8 0.10938640338162742 1107 9 0.12508183426006525 1107 10-11 0.0441140406816416 1107 12-13 0.20848378536238243 1107 14-15 0.20258941796416963 1107 16-17 0.1968818973708224 1107 18-19 0.19975347671566368 1107 20-21 0.2236349246583984 1107 22-23 0.2346268551730688 1107 24-25 0.05904560566182937 1107 26-27 0.2759523116381004 1107 28-29 0.34675472766174664 1107 30-31 0.24450763671265463 1107 32-33 0.1474996407593281 1107 34-35 0.0655465012181935 1107 36-37 0.09775413845969894 1107 38-39 0.2824657607878649 1107 40-41 0.13909485074051986 1107 42-43 0.2546935207520704 1107 44-45 0.04876762854630812 1107 46-47 -0.33061873429099364 1107 48-49 -0.26258488679332004 1107 50-51 -0.24089686613503147 1107 52-53 -0.1726564876281671 1107 54-55 -0.27509855777874037 1107 56-57 0.42800810315171844 1107 58-59 -6.96015558386129 1107 60-61 -9.371440238538053 1107 62-63 -3.073125260114857 1107 64-65 -0.704780386831434 1107 66-67 -1.9899693673814838 1107 68-69 -4.364701121026243 1107 70-71 -4.758389801830392 1107 72-73 -6.219254254745858 1107 74-75 -7.057622059204917 1107 76-77 -9.066959514429993 1107 78-79 -12.414689915258663 1107 80-81 -8.327162653937712 1107 82-83 -4.333252244253547 1107 84-85 -3.3601683563205427 1107 86-87 -3.0915686846769965 1107 88-89 -3.1940872437596504 1107 90-91 -8.575278991632885 1107 92-93 -11.839082512745959 1107 94-95 -12.10358846064906 1107 96-97 -12.32286722371246 1107 98-99 -11.649333924554124 1107 100-101 -11.20971207268181 1108 1 0.02903937625596953 1108 2 -0.04383842547881045 1108 3 -0.06826951311234453 1108 4 -0.05142608176893049 1108 5 -0.20040417098150698 1108 6 0.01965792702944924 1108 7 -0.042119106088314595 1108 8 -0.0399090631173209 1108 9 0.02439357266356268 1108 10-11 0.057530540004620434 1108 12-13 -0.03159737547186836 1108 14-15 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1.276502867477138 2314 64-65 1.1530384025858673 2314 66-67 1.972530232715311 2314 68-69 0.14385369427000683 2314 70-71 2.743678207695142 2314 72-73 8.59823743639274 2314 74-75 10.220932371754827 2314 76-77 9.206889692967216 2314 78-79 6.650889057626721 2314 80-81 3.778532354218168 2314 82-83 2.2087768850181426 2314 84-85 1.7993140089679045 2314 86-87 1.7422628904778605 2314 88-89 1.9546417511230914 2314 90-91 3.1409055689040883 2314 92-93 5.303402624274302 2314 94-95 9.004906406740886 2314 96-97 9.721085305790735 2314 98-99 10.135403815165036 2314 100-101 9.147149740308636 2315 1 -0.7625350130964321 2315 2 -0.5790081043214101 2315 3 -0.6825470329569114 2315 4 -0.5535782007867169 2315 5 -0.7893673369685388 2315 6 -0.9157008708891681 2315 7 -0.9027223894714993 2315 8 -0.9011832124588537 2315 9 -1.3928835439282423 2315 10-11 -1.006209597015065 2315 12-13 -0.7850696620875439 2315 14-15 -1.0357606370249997 2315 16-17 -1.1591838917181576 2315 18-19 -1.0477080252421374 2315 20-21 -1.0823737386661847 2315 22-23 -1.137182704621189 2315 24-25 -1.1026054638719174 2315 26-27 -1.1444510322432535 2315 28-29 -1.0692755714838356 2315 30-31 -1.2536870541897258 2315 32-33 -1.1449214121089923 2315 34-35 -1.2632229558396446 2315 36-37 -1.240945868062532 2315 38-39 -1.3795396459183351 2315 40-41 -1.1544190135025758 2315 42-43 -1.0338884108634332 2315 44-45 -1.0970787211398374 2315 46-47 -1.276181591748717 2315 48-49 -1.0643458896878428 2315 50-51 -1.014681987797161 2315 52-53 -0.8887911425008426 2315 54-55 -0.9049908509733555 2315 56-57 0.38991185298585407 2315 58-59 3.8011917520314533 2315 60-61 3.4416399871761882 2315 62-63 0.8462651135191273 2315 64-65 0.7997681959323266 2315 66-67 1.4812445326214423 2315 68-69 5.7637836068279675 2315 70-71 4.853767190565586 2315 72-73 8.740699234986124 2315 74-75 9.689264586635584 2315 76-77 8.448022729353081 2315 78-79 5.819943029214642 2315 80-81 2.988546931270765 2315 82-83 1.3839998490458711 2315 84-85 0.7673526553902406 2315 86-87 0.8418233487528219 2315 88-89 0.8057530739855459 2315 90-91 1.9562320523067243 2315 92-93 4.19486481010232 2315 94-95 8.005739569434176 2315 96-97 8.788959833658451 2315 98-99 9.154248355663496 2315 100-101 8.15041954148088 2316 1 -0.5478053417933495 2316 2 -0.45990213462859586 2316 3 -0.5790544304829837 2316 4 -0.39116902215992866 2316 5 -0.5631169283382675 2316 6 -0.49299684574917535 2316 7 -0.6332214983938727 2316 8 -0.6624777531584272 2316 9 -0.7703999155023382 2316 10-11 -0.6382488466117096 2316 12-13 -0.7266070609844988 2316 14-15 -0.8623854037102987 2316 16-17 -0.974302684492514 2316 18-19 -1.0391626435550947 2316 20-21 -0.8281788193904376 2316 22-23 -0.7452034577663227 2316 24-25 -2.364631848205825 2316 26-27 -1.458852744799536 2316 28-29 -1.0807425277946692 2316 30-31 -0.7547310386248682 2316 32-33 -0.7282508788333075 2316 34-35 -0.8333961096217166 2316 36-37 -0.7257859486286051 2316 38-39 -1.0582439452360504 2316 40-41 -0.8727356676906339 2316 42-43 -1.0216848655344748 2316 44-45 -0.954949203798094 2316 46-47 -0.7820305409098367 2316 48-49 -1.0851833972013694 2316 50-51 -0.9307656752767315 2316 52-53 -0.8371993298696836 2316 54-55 -0.8056952768346477 2316 56-57 0.2624239537145385 2316 58-59 3.8211967083212066 2316 60-61 3.6033890301904563 2316 62-63 1.197714564636403 2316 64-65 0.9398661617444724 2316 66-67 6.241322673274617 2316 68-69 8.663732462135943 2316 70-71 5.607291956196351 2316 72-73 9.237785569335323 2316 74-75 10.023394968691704 2316 76-77 8.605037988398308 2316 78-79 6.032842564167034 2316 80-81 2.9382616809781332 2316 82-83 1.3680316325720092 2316 84-85 1.1934939201932018 2316 86-87 1.1643333404220364 2316 88-89 1.149783908434923 2316 90-91 2.688346173514372 2316 92-93 4.575738804344589 2316 94-95 8.416955020403819 2316 96-97 9.318369613367187 2316 98-99 9.554581587058953 2316 100-101 8.602342476448031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 47.0 5 95.0 6 238.0 7 575.0 8 1264.0 9 2361.0 10 3911.0 11 5951.0 12 8430.0 13 11355.0 14 14965.0 15 21063.0 16 28215.0 17 35861.0 18 37483.0 19 36054.0 20 37292.0 21 37234.0 22 35502.0 23 34351.0 24 35379.0 25 37785.0 26 49522.0 27 71298.0 28 100113.0 29 130814.0 30 153545.0 31 162984.0 32 166274.0 33 171437.0 34 173894.0 35 168053.0 36 140172.0 37 87066.0 38 27489.0 39 1205.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.214030433824696 20.1847243354769 15.21947536080589 22.381769869892516 2 15.589855040324071 21.346649024115592 40.01010257812866 23.053393357431677 3 18.87061539136293 26.147073592975463 30.382770993318154 24.59954002234346 4 12.788382172133673 17.912476993241494 37.22879127973015 32.070349554894676 5 13.07253131774169 38.459478757586574 34.01725113958906 14.450738785082676 6 30.62472034581519 37.25910317490041 17.669857270417467 14.446319208866933 7 27.76545549069997 33.71885309922904 20.72705052739991 17.788640882671075 8 26.05709594409068 35.65909388819516 19.567374928915886 18.716435238798276 9 26.744017010833808 16.649181119551372 20.182378609319983 36.42442326029483 10-11 24.774786317415654 27.399712708107987 27.086278732269086 20.73922224220727 12-13 25.78218051096563 24.85535617049271 27.857260772829974 21.50520254571169 14-15 22.61990089639704 25.898386652872023 26.611887258132832 24.869825192598103 16-17 21.426948767921864 29.413195222971524 27.054445016619074 22.10541099248754 18-19 21.338369963144327 27.322993967772604 30.213003020302402 21.125633048780667 20-21 22.495891430275048 26.44595783264631 30.159759916621287 20.89839082045735 22-23 22.05751258441971 26.545302679009215 29.959345486979466 21.437839249591605 24-25 21.64611355175073 26.797533133428114 30.243557491641187 21.31279582317997 26-27 21.440773077316354 27.064136885145935 30.135847838593993 21.359242198943722 28-29 21.2584458252454 27.053670382805134 30.37872975182542 21.309154040124046 30-31 21.445346718363638 26.88636646448667 30.31098264898916 21.357304168160532 32-33 21.307670473584825 26.9782800667032 30.101099498937558 21.61294996077442 34-35 21.748447294496746 26.950611312728824 29.978556439092575 21.32238495368186 36-37 21.714505978106565 26.72407844096028 29.9708291271018 21.59058645383135 38-39 21.33511505091302 27.055818497461985 30.079595653292614 21.529470798332376 40-41 21.49944878643056 26.835782719571665 30.105182837035738 21.55958565696204 42-43 21.472874980467775 26.80478967062142 29.900788633503538 21.821546715407266 44-45 21.460030857351295 27.109563314256985 29.65699540097304 21.77341042741868 46-47 21.50269168753848 27.27642430716342 29.19676258088495 22.024121424413146 48-49 21.616108687352565 27.306386111514364 29.08658959221293 21.990915608920144 50-51 21.584534004876378 27.432143912502237 28.885716179903937 22.097605902717447 52-53 21.7501852051748 27.602299057930402 28.62732282777619 22.020192909118602 54-55 21.44169040421477 27.44127097720333 28.803743398398733 22.313295220183164 56-57 21.593599680980983 27.56200426263497 28.774390037762593 22.070006018621452 58-59 20.36144799623887 27.014323182346907 30.313232424177883 22.310996397236345 60-61 21.745907353874845 27.30445738287634 28.876024598013565 22.07361066523525 62-63 21.299712017219832 27.374675255557612 28.88466299509976 22.440949732122796 64-65 20.604413499069828 27.14642414791933 29.475773620658174 22.773388732352664 66-67 21.111285998745803 27.02028946401937 29.406081592576772 22.462342944658058 68-69 21.971370432986767 26.31579080573806 29.438544629931464 22.274294131343705 70-71 21.129357509188313 26.756329468496613 29.62138129494229 22.492931727372778 72-73 20.948098097872045 26.72358547330883 29.62874304385639 22.699573384962733 74-75 21.386179683528344 26.89888998747083 28.91056253177326 22.80436779722757 76-77 19.6849609115677 27.461171260467736 29.659006581125407 23.19486124683916 78-79 20.564396324274313 27.618484511509216 28.613241073228778 23.203878090987693 80-81 20.88606830811064 27.864467901466057 27.970243787175864 23.279220003247442 82-83 21.266792507036325 27.785129827395338 27.580156709943697 23.367920955624637 84-85 21.226544798338733 27.43597034239494 27.65240029290975 23.685084566356576 86-87 21.193468872028685 27.41585995911762 27.78407735719256 23.606593811661135 88-89 21.29102535167403 27.384425322304214 27.697711617932146 23.626837708089614 90-91 21.021384211837603 27.522354493010493 27.78690704387834 23.669354251273564 92-93 20.802905746609003 27.301470717080395 27.92383101672749 23.97179251958311 94-95 19.779212665067764 27.238763515071305 28.736038293824862 24.24598552603607 96-97 19.65704484001887 27.213443798365976 28.887532370793107 24.241978990822048 98-99 19.913192988863514 27.19386608901475 28.318685127047182 24.57425579507455 100-101 19.64227453240542 27.30433255457589 28.46645798110946 24.586934931909227 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 687.0 1 512.5 2 368.0 3 422.0 4 461.5 5 532.5 6 717.0 7 1091.5 8 1561.0 9 1944.0 10 2171.0 11 2353.0 12 2631.0 13 3025.0 14 3359.5 15 3597.0 16 3865.5 17 4037.0 18 4247.5 19 4648.0 20 5146.0 21 5834.5 22 6638.5 23 7594.5 24 8826.5 25 10576.5 26 13018.0 27 15837.5 28 18520.0 29 21466.0 30 25066.5 31 28846.0 32 33190.5 33 38153.0 34 42968.5 35 47531.5 36 52026.5 37 56196.5 38 59644.0 39 62472.5 40 65029.0 41 67951.5 42 71185.5 43 74062.0 44 76721.0 45 78334.5 46 78931.5 47 79454.5 48 79864.0 49 79215.5 50 76928.0 51 74094.0 52 70643.0 53 66589.5 54 61964.0 55 56314.5 56 50099.5 57 43502.5 58 37209.0 59 31479.0 60 25922.0 61 20551.5 62 15788.0 63 11971.0 64 8939.0 65 6717.0 66 5032.5 67 3722.0 68 2710.0 69 1876.0 70 1343.0 71 1015.0 72 801.5 73 634.0 74 490.5 75 352.5 76 199.0 77 96.5 78 51.0 79 27.5 80 16.5 81 12.5 82 7.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021386737758679737 2 0.005420601736070902 3 0.0032523610416425406 4 0.0 5 0.06756040891048369 6 1.478345928019337E-4 7 0.0 8 4.4350377840580106E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.2175188920290053E-4 16-17 0.0 18-19 0.0013797895328180477 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.231954940016114E-4 28-29 0.0018725715088244933 30-31 0.00800770711010474 32-33 3.449473832045119E-4 34-35 7.145338652093461E-4 36-37 0.027546512458760306 38-39 0.026659504901948704 40-41 0.02904949748557997 42-43 0.029369805769984155 44-45 0.029813309548389955 46-47 0.031636602859613804 48-49 0.032055467539219284 50-51 0.026339196617544518 52-53 0.022865083686699074 54-55 0.016582113492616894 56-57 0.15194932230158747 58-59 0.7395671895904735 60-61 0.30175504300754696 62-63 3.449473832045119E-4 64-65 0.1372397803177951 66-67 1.3802823147940542 68-69 2.6469783841186225 70-71 2.1428377835652284 72-73 1.8051589345068115 74-75 1.515748079998226 76-77 1.0898119790370548 78-79 0.40395802483128374 80-81 2.2175188920290053E-4 82-83 2.2175188920290053E-4 84-85 4.9278197600644565E-5 86-87 0.002907413658438029 88-89 4.9278197600644565E-5 90-91 0.21517324982321448 92-93 0.16648639059377762 94-95 1.2527010612059855 96-97 1.2309940151629013 98-99 0.9633641239938009 100-101 0.38991373851510003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2029295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.823703480429316 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98003085546615 37.59939151671223 2 13.940077081117133 14.169726881300516 3 4.97572360017054 7.586541025669254 4 2.3675501118996625 4.8131065944898275 5 1.2481329806724761 3.1717370256921167 6 0.7686591721393732 2.3439663505394264 7 0.5260215267992725 1.8714053471658247 8 0.34865464641138627 1.417593629302898 9 0.25220067634975946 1.153599515312751 >10 1.401839862556313 13.258703357116538 >50 0.12046677990944987 4.235268233973976 >100 0.06792285994757759 5.951002321830451 >500 0.0018456101663066942 0.6147898226054326 >1k 5.828242630442183E-4 0.45305759271260326 >5k 1.9427475434807275E-4 0.7885680427588984 >10k+ 9.713737717403638E-5 0.5715427428172395 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11577 0.570493693622662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9397 0.4630672228532569 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6576 0.3240534274218386 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2161 0.1064901850149929 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.927819760064456E-5 0.0 20-21 0.0 0.0 0.0 1.2319549400161142E-4 0.0 22-23 0.0 0.0 0.0 4.6814287720612334E-4 0.0 24-25 0.0 0.0 0.0 9.116466556119242E-4 0.0 26-27 0.0 0.0 0.0 0.002389992583631261 0.0 28-29 0.0 0.0 0.0 0.009264301148921177 0.0 30-31 0.0 0.0 0.0 0.031020625389605747 0.0 32-33 0.0 0.0 0.0 0.07534636413138553 0.0 34-35 0.0 0.0 0.0 0.13820070517100766 0.0 36-37 0.0 0.0 0.0 0.24868242419165276 0.0 38-39 0.0 0.0 0.0 0.41967776986588934 0.0 40-41 0.0 0.0 0.0 0.6118381014096028 0.0 42-43 2.463909880032228E-5 0.0 0.0 0.7966313424120199 0.0 44-45 4.927819760064456E-5 0.0 0.0 1.0151801487708785 0.0 46-47 4.927819760064456E-5 0.0 0.0 1.265069888803747 0.0 48-49 4.927819760064456E-5 4.927819760064456E-5 0.0 1.5099086135825495 0.0 50-51 4.927819760064456E-5 4.927819760064456E-5 0.0 1.761621646926642 0.0 52-53 9.855639520128912E-5 4.927819760064456E-5 0.0 2.0007933789813706 0.0 54-55 1.2319549400161142E-4 9.855639520128912E-5 0.0 2.2402607802217025 0.0 56-57 1.7247369160225596E-4 9.855639520128912E-5 0.0 2.4527976464732824 0.0 58-59 1.9711279040257823E-4 9.855639520128912E-5 0.0 2.6083689162985175 0.0 60-61 1.9711279040257823E-4 9.855639520128912E-5 0.0 2.7274743198992755 0.0 62-63 1.9711279040257823E-4 9.855639520128912E-5 0.0 2.8307367829714263 0.0 64-65 1.9711279040257823E-4 1.2319549400161142E-4 0.0 2.9341224415375784 0.0 66-67 1.9711279040257823E-4 1.478345928019337E-4 0.0 3.0178953774586743 0.0 68-69 1.9711279040257823E-4 1.478345928019337E-4 0.0 3.1168706373395687 0.0 70-71 1.9711279040257823E-4 1.478345928019337E-4 0.0 3.302329134009595 0.0 72-73 1.9711279040257823E-4 1.478345928019337E-4 0.0 3.5312510009633886 0.0 74-75 1.9711279040257823E-4 1.478345928019337E-4 0.0 3.79126741060319 0.0 76-77 1.9711279040257823E-4 1.478345928019337E-4 0.0 4.155605764563555 0.0 78-79 1.9711279040257823E-4 1.478345928019337E-4 0.0 4.672952922073922 0.0 80-81 1.9711279040257823E-4 1.478345928019337E-4 0.0 5.260201202880803 0.0 82-83 1.9711279040257823E-4 1.478345928019337E-4 0.0 5.803025188550704 0.0 84-85 1.9711279040257823E-4 1.478345928019337E-4 4.927819760064456E-5 6.239802492984016 0.0 86-87 1.9711279040257823E-4 1.478345928019337E-4 4.927819760064456E-5 6.62158532889501 0.0 88-89 1.9711279040257823E-4 1.478345928019337E-4 4.927819760064456E-5 7.0154413232181625 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5270 0.0 55.135803 1 GTATCAA 9775 0.0 42.368332 1 TATCAAC 12750 0.0 32.178486 2 ATCAACG 12795 0.0 32.10138 3 TCAACGC 12970 0.0 31.631107 4 CAACGCA 13230 0.0 31.045126 5 AACGCAG 13625 0.0 30.093428 6 TGGTATC 2530 0.0 26.468195 2 ACGCAGA 15590 0.0 26.20969 7 CGCAGAG 15545 0.0 26.194609 8 GTACATG 11370 0.0 25.638441 1 TACATGG 11345 0.0 25.189796 2 GTGGTAT 2850 0.0 24.660679 1 GCAGAGT 16450 0.0 24.295109 9 ACATGGG 11435 0.0 24.289856 3 GAGTACT 9245 0.0 22.812614 12-13 CATGGGA 6915 0.0 22.29927 4 GTACTTT 9800 0.0 21.400446 14-15 AGTACTT 9545 0.0 20.762466 12-13 CAGAGTA 16260 0.0 20.622593 10-11 >>END_MODULE