FastQCFastQC Report
Fri 27 May 2016
SRR939888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099013
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106500.5073813263662493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78450.3737470897035893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54900.26155150063386934No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21650.10314371564158964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53850.051.9667321
GTATCAA103650.044.1507071
TATCAAC131800.034.6138952
ATCAACG132500.034.325363
TCAACGC133650.034.064384
CAACGCA136550.033.1703035
AACGCAG140500.032.205826
ACGCAGA161600.027.8260867
CGCAGAG161800.027.6463388
GTACATG121450.027.4176271
TACATGG120200.027.1158312
GCAGAGT165950.025.311039
ACATGGG125100.024.8512743
GAGTACT90650.023.58229812-13
TGGTATC24650.023.468342
CATGGGG49950.022.315524
GTACTTT97300.021.88040214-15
GTGGTAT28350.021.7326341
AGTACTT92700.021.56399712-13
CATGGGA71700.021.4497764
Produced by FastQC (version 0.11.5)