##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939888_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099013 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.013174287153056 31.0 30.0 33.0 26.0 34.0 2 30.061926248193792 31.0 30.0 33.0 26.0 34.0 3 29.992147738008292 31.0 30.0 34.0 25.0 34.0 4 33.647013143796634 35.0 33.0 37.0 28.0 37.0 5 33.52359180243286 35.0 33.0 37.0 28.0 37.0 6 33.509071168210966 35.0 33.0 37.0 28.0 37.0 7 33.50874053662364 35.0 33.0 37.0 28.0 37.0 8 33.3500516671407 35.0 33.0 37.0 26.0 37.0 9 34.63265401405327 37.0 34.0 39.0 27.0 39.0 10-11 34.421507870603946 37.0 33.0 39.0 27.0 39.0 12-13 34.31589751945319 37.0 33.0 39.0 27.0 39.0 14-15 34.87608842822793 38.0 33.0 40.0 25.0 41.0 16-17 34.68820083534499 38.0 33.0 40.0 25.0 41.0 18-19 34.76754145877133 38.0 33.0 40.0 25.0 41.0 20-21 34.92169700711716 38.0 33.5 40.0 25.0 41.0 22-23 34.83448101560114 38.0 33.5 40.0 25.0 41.0 24-25 34.65663099752122 38.0 33.0 40.0 25.0 41.0 26-27 34.52945741641429 38.0 33.0 40.0 24.0 41.0 28-29 34.4006754603235 38.0 33.0 40.0 24.0 41.0 30-31 34.183255177552496 38.0 33.0 40.0 23.5 41.0 32-33 33.87616894226001 38.0 32.5 40.0 22.5 41.0 34-35 33.58682819020177 37.5 32.0 40.0 19.5 41.0 36-37 33.35480223324009 37.0 31.5 40.0 19.0 41.0 38-39 33.073578391367754 37.0 31.0 40.0 18.0 41.0 40-41 32.76305839935246 37.0 31.0 40.0 15.5 41.0 42-43 32.41284832442676 37.0 30.5 40.0 14.5 41.0 44-45 31.959630073753715 37.0 30.0 39.0 9.0 41.0 46-47 31.50299569369032 36.0 30.0 39.0 8.5 40.5 48-49 31.124720523407905 36.0 29.5 39.0 7.5 40.0 50-51 30.386010948955533 35.5 28.0 38.5 2.0 40.0 52-53 30.49061773319174 35.5 28.0 38.5 2.0 40.0 54-55 30.568910959579576 36.0 28.0 39.0 2.0 40.0 56-57 30.34959716781173 35.0 28.0 39.0 2.0 40.0 58-59 29.82818686687505 35.0 27.0 39.0 2.0 40.0 60-61 29.337486475786477 34.5 26.0 38.0 2.0 40.0 62-63 28.903589448945766 34.0 26.0 38.0 2.0 40.0 64-65 28.379495982159234 34.0 25.5 38.0 2.0 40.0 66-67 27.843261809240822 33.5 24.5 37.0 2.0 39.0 68-69 27.310526900023966 33.0 23.0 36.5 2.0 39.0 70-71 26.765574581958283 32.5 22.0 36.0 2.0 39.0 72-73 26.281501591462273 32.0 20.5 36.0 2.0 38.5 74-75 25.677217577975934 31.5 19.0 35.0 2.0 38.0 76-77 25.024240678833337 31.0 17.0 35.0 2.0 37.0 78-79 24.3651461424965 31.0 10.5 34.5 2.0 36.5 80-81 23.679953149408796 30.0 7.0 34.0 2.0 36.0 82-83 22.93169027538181 29.5 2.0 34.0 2.0 35.5 84-85 22.18777444446509 29.0 2.0 34.0 2.0 35.0 86-87 21.392978033008845 29.0 2.0 34.0 2.0 35.0 88-89 20.543014026116083 27.0 2.0 33.0 2.0 35.0 90-91 19.60676517963443 26.0 2.0 32.5 2.0 34.0 92-93 18.63448868587284 25.0 2.0 32.0 2.0 34.0 94-95 17.27477295281163 21.5 2.0 32.0 2.0 34.0 96-97 14.729227737036407 2.0 2.0 30.5 2.0 34.0 98-99 13.356777923719385 2.0 2.0 30.0 2.0 34.0 100-101 12.104187777779366 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8214453639275163 1101 2 0.781857962278643 1101 3 1.0034241446530565 1101 4 1.0108867465501135 1101 5 1.0512643754208426 1101 6 0.9859713115578046 1101 7 0.8321680999383148 1101 8 0.8182627911290936 1101 9 0.9028231136882496 1101 10-11 0.9052524215876545 1101 12-13 0.7161904434465711 1101 14-15 0.9474953242638904 1101 16-17 0.8585329583074426 1101 18-19 0.8539513280759436 1101 20-21 0.9551523050052424 1101 22-23 0.8525527817212861 1101 24-25 0.9864759400705196 1101 26-27 1.0352793713982678 1101 28-29 1.311205838455514 1101 30-31 1.0571236554925392 1101 32-33 1.162906167888039 1101 34-35 0.9898331926006421 1101 36-37 1.0435263607681335 1101 38-39 1.0467214649790577 1101 40-41 0.9471445979824082 1101 42-43 0.8730789969825707 1101 44-45 0.7495926038935252 1101 46-47 0.6065979084769602 1101 48-49 0.3772940601530159 1101 50-51 0.7195595464042661 1101 52-53 0.7341144772484824 1101 54-55 0.5814515092175476 1101 56-57 0.25092187341777006 1101 58-59 0.13771958772261783 1101 60-61 0.3488966301229759 1101 62-63 0.3646245478802328 1101 64-65 0.3631748821135865 1101 66-67 0.10535914540257352 1101 68-69 0.24284602604240746 1101 70-71 0.30160823851834095 1101 72-73 0.023849720097526728 1101 74-75 -0.030947205646256748 1101 76-77 -0.40233425851644355 1101 78-79 -0.18401045949176265 1101 80-81 -0.37874089266304267 1101 82-83 -0.35170459488752925 1101 84-85 -0.40010277410146955 1101 86-87 -0.5905866416282493 1101 88-89 -0.7609855672429191 1101 90-91 -0.7126944494971923 1101 92-93 -0.8161966266985168 1101 94-95 -0.7320674729787555 1101 96-97 0.7547834609177357 1101 98-99 0.8002144063292018 1101 100-101 0.13001761012340118 1102 1 0.9777621203147682 1102 2 0.763465490859069 1102 3 0.9337841298685348 1102 4 1.0721401719290213 1102 5 1.0375568983080399 1102 6 1.0562864662885545 1102 7 0.8512990522483008 1102 8 1.0184529629991559 1102 9 1.178794639574079 1102 10-11 0.9192203417591855 1102 12-13 0.7603748505455243 1102 14-15 1.2150649843987296 1102 16-17 1.1075977478962784 1102 18-19 1.1335130435176168 1102 20-21 0.9885572011856993 1102 22-23 0.912552553197095 1102 24-25 0.9953208198369836 1102 26-27 1.3233389575906358 1102 28-29 1.3800821601698843 1102 30-31 1.4910889824113056 1102 32-33 1.504161962963444 1102 34-35 1.2151559333409452 1102 36-37 0.9675603244307993 1102 38-39 1.0286438353114562 1102 40-41 1.1882453195227 1102 42-43 1.164257441587317 1102 44-45 0.8954398473949894 1102 46-47 1.0041428134804526 1102 48-49 0.9596047477326266 1102 50-51 1.0950136140355013 1102 52-53 1.15789532553028 1102 54-55 0.994414842014347 1102 56-57 0.6637805073597711 1102 58-59 0.4867789871213013 1102 60-61 0.8192597749337871 1102 62-63 0.7606497670150603 1102 64-65 0.4140390264693359 1102 66-67 0.3873581210180461 1102 68-69 0.2819540911578926 1102 70-71 0.5023084912859694 1102 72-73 0.3681068823437421 1102 74-75 -0.023665977815589656 1102 76-77 -0.252685067988871 1102 78-79 -0.39824654783325286 1102 80-81 -0.23571679230316533 1102 82-83 -0.23074599552222708 1102 84-85 -0.22243043208620605 1102 86-87 -0.4614051457287687 1102 88-89 -0.46390685158861444 1102 90-91 -0.5931287299142625 1102 92-93 -0.4939787596836993 1102 94-95 -0.39124885461614056 1102 96-97 0.9477946198533296 1102 98-99 0.7364626153950535 1102 100-101 0.4304266932644758 1103 1 0.5993773246671275 1103 2 0.6606109655581349 1103 3 0.8473025695608385 1103 4 0.8313242461847565 1103 5 0.8940006772511637 1103 6 1.0401026842099839 1103 7 0.8013125087233846 1103 8 0.5500019749385672 1103 9 0.9102509326789132 1103 10-11 0.7359474725337876 1103 12-13 0.650662684667644 1103 14-15 0.7280056479457997 1103 16-17 0.8616421929169178 1103 18-19 0.7580170085562301 1103 20-21 0.7761009467337274 1103 22-23 0.7809673132096862 1103 24-25 0.8609578560897617 1103 26-27 1.1695650585427657 1103 28-29 1.0388277526040994 1103 30-31 0.957206154338003 1103 32-33 0.9595947416883348 1103 34-35 1.1429481999371234 1103 36-37 0.9793310789129137 1103 38-39 1.0128593060083801 1103 40-41 1.090174505092655 1103 42-43 1.0187990679505745 1103 44-45 0.9144481688023589 1103 46-47 0.7217535821176675 1103 48-49 0.5603428491956493 1103 50-51 0.8687538490880691 1103 52-53 0.9940622011864484 1103 54-55 0.7321788728204304 1103 56-57 0.7619430322890643 1103 58-59 0.7053463538847922 1103 60-61 0.6623228239813805 1103 62-63 0.5506795393161354 1103 64-65 0.4941701148640938 1103 66-67 0.4412686495033995 1103 68-69 0.16713086554598178 1103 70-71 0.19750660637112105 1103 72-73 -0.041847932229391205 1103 74-75 -0.09697039593287826 1103 76-77 0.004731778557616195 1103 78-79 -0.005891506240619293 1103 80-81 -0.02453440148727637 1103 82-83 -0.10121427845439968 1103 84-85 -0.23112336300730973 1103 86-87 -0.10040393116485191 1103 88-89 -0.23913762233373248 1103 90-91 -0.5944087155862974 1103 92-93 -0.6712726805500218 1103 94-95 -0.47915516873996467 1103 96-97 1.003594936806735 1103 98-99 1.3551488456878005 1103 100-101 0.921811788291679 1104 1 0.35344658819781927 1104 2 0.39109405193642033 1104 3 0.39092714811991414 1104 4 0.6839951345179429 1104 5 0.6217342892642534 1104 6 0.7630153852886252 1104 7 0.6153435367282114 1104 8 0.5033106749721554 1104 9 0.7162491826364104 1104 10-11 0.7657106362125319 1104 12-13 0.6140082102402431 1104 14-15 0.8844561304516034 1104 16-17 0.7244338839473272 1104 18-19 0.9136795572318803 1104 20-21 0.6139234293888123 1104 22-23 0.6547859308419888 1104 24-25 0.8024091887346927 1104 26-27 0.991769863280318 1104 28-29 1.034520035414289 1104 30-31 0.8611238442818845 1104 32-33 0.9510695049971574 1104 34-35 1.0060840936407018 1104 36-37 0.9351476893874562 1104 38-39 0.7635653361903962 1104 40-41 0.7645981729661031 1104 42-43 0.6705831160228328 1104 44-45 0.7610022024015208 1104 46-47 0.4477817320905011 1104 48-49 0.5760019973147692 1104 50-51 0.8634155973888298 1104 52-53 0.7535018558226092 1104 54-55 0.624551258642601 1104 56-57 0.4526468645503918 1104 58-59 0.36720662418756334 1104 60-61 0.670749568943787 1104 62-63 0.503543471437709 1104 64-65 0.5370834794659594 1104 66-67 0.45801120663897166 1104 68-69 0.2817130325298969 1104 70-71 0.4375029569933808 1104 72-73 0.5673835419948041 1104 74-75 0.4188276773615556 1104 76-77 0.1601600205943008 1104 78-79 0.12323633394981215 1104 80-81 0.15490508726808017 1104 82-83 0.1746672743558264 1104 84-85 -0.03380710710299084 1104 86-87 0.1420433083711785 1104 88-89 0.08596491273969065 1104 90-91 -0.1301270002128767 1104 92-93 0.06864902370938353 1104 94-95 0.3818118334206311 1104 96-97 1.6830199109915327 1104 98-99 1.768686585644753 1104 100-101 1.703673344170701 1105 1 0.5667831155006482 1105 2 0.7858747379310884 1105 3 0.7751764822983951 1105 4 0.7359229328636303 1105 5 0.6151258676818117 1105 6 1.005718936919905 1105 7 0.8635553399707163 1105 8 0.7538587412972646 1105 9 1.0421273784563425 1105 10-11 0.9698387072119559 1105 12-13 0.7566524805415753 1105 14-15 0.7646208172609121 1105 16-17 0.9136938474948053 1105 18-19 0.9028783777958438 1105 20-21 0.9078327717944532 1105 22-23 0.7287673708287841 1105 24-25 0.7431654283021416 1105 26-27 0.9650360694232418 1105 28-29 1.0081970389684756 1105 30-31 1.0376180231379024 1105 32-33 1.0953536005094051 1105 34-35 1.0746418414615562 1105 36-37 0.9516523697639698 1105 38-39 0.9387635350069274 1105 40-41 0.8046376310499639 1105 42-43 0.7582606067470934 1105 44-45 0.9752805469743038 1105 46-47 0.9159944459731939 1105 48-49 0.8449571072398072 1105 50-51 0.8675826733949847 1105 52-53 1.0589420112863799 1105 54-55 0.9925140399261174 1105 56-57 0.8599312458152504 1105 58-59 0.654857221868081 1105 60-61 0.720453476307906 1105 62-63 0.7072266520930199 1105 64-65 0.7874260910115289 1105 66-67 0.5704771753874169 1105 68-69 0.5086609389770942 1105 70-71 0.6376547154036807 1105 72-73 0.660029180499361 1105 74-75 0.5704799958379958 1105 76-77 0.5243043933987259 1105 78-79 0.4860921284699131 1105 80-81 0.3693812605696358 1105 82-83 0.32112726158892 1105 84-85 0.26287957570398746 1105 86-87 0.15175328966044077 1105 88-89 0.2292903688111707 1105 90-91 0.0895678590662996 1105 92-93 -0.12570603893414045 1105 94-95 0.09811354869888689 1105 96-97 1.295650400570537 1105 98-99 1.3994147030285156 1105 100-101 0.8788382350890149 1106 1 0.9278576864654475 1106 2 1.0896017706810568 1106 3 1.0913459206018672 1106 4 1.0100522139478443 1106 5 0.9472391927553332 1106 6 0.9956155152234061 1106 7 0.9545183102563897 1106 8 0.9306273530292088 1106 9 1.4368481575953709 1106 10-11 1.3432871369545438 1106 12-13 1.2228553062541678 1106 14-15 1.4028223084097462 1106 16-17 1.4779756847908772 1106 18-19 1.4268621718458903 1106 20-21 1.3435749491851183 1106 22-23 1.1462829838724389 1106 24-25 1.1579922124651318 1106 26-27 1.4472636857534198 1106 28-29 1.3926176158711954 1106 30-31 1.4333192076914685 1106 32-33 1.553961256951105 1106 34-35 1.488519695770286 1106 36-37 1.6468396633367561 1106 38-39 1.6470093390815919 1106 40-41 1.616452134148794 1106 42-43 1.6999935158675896 1106 44-45 1.899399382835547 1106 46-47 1.8456097306917876 1106 48-49 1.5711003811923518 1106 50-51 1.754390380104315 1106 52-53 1.764592014247416 1106 54-55 1.6883950781626247 1106 56-57 1.7495290860788764 1106 58-59 1.7423297856713695 1106 60-61 1.7808252329845722 1106 62-63 1.5669337848280236 1106 64-65 1.5356240568976212 1106 66-67 1.567060095302864 1106 68-69 1.7295324063117583 1106 70-71 1.8675172348194415 1106 72-73 1.9648543605745417 1106 74-75 1.962786366351505 1106 76-77 1.822280141829264 1106 78-79 1.6064205141560386 1106 80-81 1.7485654700038147 1106 82-83 1.7452513433768857 1106 84-85 1.8750883160240868 1106 86-87 2.006420835466706 1106 88-89 1.9670068565489913 1106 90-91 1.736514776349928 1106 92-93 2.0939776340166034 1106 94-95 2.564533788929687 1106 96-97 3.951188280591296 1106 98-99 4.055386129748049 1106 100-101 3.7305096572853955 1107 1 0.5296505549581205 1107 2 0.5772801841253568 1107 3 0.4747829318354455 1107 4 0.12756383357986323 1107 5 0.49972863920810795 1107 6 0.6421225645360593 1107 7 0.6697582924052412 1107 8 0.49126334875933253 1107 9 0.663725770599207 1107 10-11 0.6978833238784361 1107 12-13 0.8608215625935642 1107 14-15 0.9393537115296411 1107 16-17 0.7118065012872208 1107 18-19 0.5615999449839748 1107 20-21 0.6539506540817683 1107 22-23 0.5474255406391464 1107 24-25 0.7190725255710007 1107 26-27 0.8488122546845673 1107 28-29 0.9138200118390856 1107 30-31 0.8751091317594657 1107 32-33 1.10036744356578 1107 34-35 0.9878093187039312 1107 36-37 0.698037462679288 1107 38-39 0.5939457759677538 1107 40-41 0.7630977514095818 1107 42-43 0.9846736927361306 1107 44-45 0.6845093395598951 1107 46-47 0.36158208079033827 1107 48-49 0.6118289740019591 1107 50-51 0.8524128389573669 1107 52-53 0.7312252907690429 1107 54-55 0.6090915222852473 1107 56-57 0.5427184425836344 1107 58-59 0.5139429242665479 1107 60-61 0.6098190012343494 1107 62-63 0.5124690816957198 1107 64-65 0.4337295411424087 1107 66-67 0.354818765087483 1107 68-69 0.038069345688452216 1107 70-71 0.4551110998937169 1107 72-73 0.4411060525803343 1107 74-75 0.3613914336233002 1107 76-77 0.38160972217490396 1107 78-79 0.08349680659924985 1107 80-81 0.19657791412089765 1107 82-83 0.07119246319050632 1107 84-85 -0.047478391578390955 1107 86-87 0.06194423925855119 1107 88-89 -0.04691962605883049 1107 90-91 0.009789807305615739 1107 92-93 0.28751344500484777 1107 94-95 0.758264471911609 1107 96-97 2.2238409672403634 1107 98-99 2.3171925573625085 1107 100-101 2.0844457715770695 1108 1 0.47779699997992253 1108 2 0.37852307707036204 1108 3 0.5319200956702375 1108 4 0.4279169281665105 1108 5 0.4867242476968343 1108 6 0.49162084176293774 1108 7 0.4125105470099726 1108 8 0.5495485900625354 1108 9 0.4348201886391081 1108 10-11 0.3494464106586932 1108 12-13 0.27478571446037137 1108 14-15 0.39831393829847883 1108 16-17 0.5882506581466131 1108 18-19 0.5297273831157767 1108 20-21 0.43495712146075505 1108 22-23 0.24752353122985937 1108 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0.9235183893180867 2311 86-87 1.2706933239691693 2311 88-89 1.310748738400708 2311 90-91 1.7080479575174365 2311 92-93 1.645180642815106 2311 94-95 2.2497276749450634 2311 96-97 3.800265297520042 2311 98-99 3.8934448599039353 2311 100-101 3.448136273524737 2312 1 0.07060370228772328 2312 2 -0.4442555581087255 2312 3 -0.5981818734654283 2312 4 -0.2177001314082645 2312 5 -1.98407590314946 2312 6 -0.5221701854179202 2312 7 -0.0510956723361673 2312 8 0.2517232907731355 2312 9 0.14316268577904623 2312 10-11 0.14422964305810382 2312 12-13 0.010635476623939155 2312 14-15 0.2952798996425301 2312 16-17 -0.4378603279634845 2312 18-19 -0.021311903470000004 2312 20-21 0.4206390829696005 2312 22-23 0.272806869792106 2312 24-25 0.23241232430842018 2312 26-27 0.5862630761767846 2312 28-29 0.5314830909023769 2312 30-31 0.27172529615533136 2312 32-33 -0.3864920338499047 2312 34-35 0.1974434034032626 2312 36-37 0.4890856615708046 2312 38-39 0.6639138474996713 2312 40-41 0.6705830065558089 2312 42-43 0.5494763998552301 2312 44-45 0.6552337748017862 2312 46-47 0.4038403287670498 2312 48-49 0.8189164359892445 2312 50-51 0.7506181515963455 2312 52-53 0.3531437064793437 2312 54-55 0.6538894341242099 2312 56-57 0.547083855581878 2312 58-59 0.8384884955596483 2312 60-61 0.7606036748105218 2312 62-63 0.8912459183417809 2312 64-65 0.7043348535283407 2312 66-67 0.8581235861153544 2312 68-69 0.8364474275890537 2312 70-71 1.0317565041111614 2312 72-73 1.0820028332266354 2312 74-75 1.1413570316004282 2312 76-77 1.025157508280106 2312 78-79 1.2216245976515623 2312 80-81 1.1669037970565945 2312 82-83 1.1360685545437548 2312 84-85 1.4671775787035237 2312 86-87 1.4115606484794085 2312 88-89 1.4644296253270923 2312 90-91 1.3921773380589855 2312 92-93 1.290697118784088 2312 94-95 1.7374353692426325 2312 96-97 3.466703876001617 2312 98-99 3.8272386059694394 2312 100-101 3.3463711840945205 2313 1 -0.2039972648800692 2313 2 -0.6440039565634663 2313 3 -1.1428756275246137 2313 4 -0.20132284281314838 2313 5 -0.29006697591206887 2313 6 -0.11363565452736424 2313 7 -0.19792769674865696 2313 8 0.06468522651211117 2313 9 0.2696617242772348 2313 10-11 -0.7792673966212575 2313 12-13 -0.18899062952659307 2313 14-15 -0.11331149263266838 2313 16-17 -0.8457941848651558 2313 18-19 -0.4290464430712291 2313 20-21 -2.6178400443654226 2313 22-23 -0.705089275106829 2313 24-25 -0.16206816931292423 2313 26-27 -0.08985336787352338 2313 28-29 -0.3661211494244796 2313 30-31 -0.09250312610860334 2313 32-33 0.08060075042022419 2313 34-35 -0.21798163847152097 2313 36-37 -0.8631945101989729 2313 38-39 -0.300758194382567 2313 40-41 -0.03145486917544815 2313 42-43 -0.15262428552609464 2313 44-45 0.04409930750726687 2313 46-47 -0.2708338121471563 2313 48-49 -0.42186390384423333 2313 50-51 -0.24313364926621617 2313 52-53 -0.25067167226687914 2313 54-55 -0.3718922637285047 2313 56-57 -0.34529861425486885 2313 58-59 -0.296759836455994 2313 60-61 -0.48785426258509546 2313 62-63 -0.3890340292086307 2313 64-65 -0.35846390818394624 2313 66-67 -0.10102281965030713 2313 68-69 -0.34746638587616374 2313 70-71 -0.07237282206731876 2313 72-73 -0.46235852369570196 2313 74-75 -0.1648971762932625 2313 76-77 -0.015092286605554506 2313 78-79 0.04948532516676707 2313 80-81 -0.06931835674377496 2313 82-83 0.14552145728719523 2313 84-85 -0.0017541269424263817 2313 86-87 0.21321573820848982 2313 88-89 0.2858681417369411 2313 90-91 0.49375304364928496 2313 92-93 0.6016515653544516 2313 94-95 0.9808814393446568 2313 96-97 2.4050695628579035 2313 98-99 2.6425004032448935 2313 100-101 2.3946740229898875 2314 1 -0.384198543836753 2314 2 -0.4146467785568859 2314 3 -1.6283888860476061 2314 4 -0.6035421662206772 2314 5 -0.6964411180200685 2314 6 -0.6621463616552958 2314 7 -0.40724590170281516 2314 8 -1.9737947278758199 2314 9 -1.633617427956274 2314 10-11 -1.0033696906235718 2314 12-13 -0.8078910309659264 2314 14-15 -0.9765494145473426 2314 16-17 -1.2177374699402037 2314 18-19 -2.1531303288849344 2314 20-21 -1.7951099953211838 2314 22-23 -2.347698719332435 2314 24-25 -2.129924856635661 2314 26-27 -2.073837338465488 2314 28-29 -3.0859157261105814 2314 30-31 -1.9602480364959334 2314 32-33 -1.7533477315982964 2314 34-35 -2.26501224676257 2314 36-37 -3.527065412264591 2314 38-39 -3.621130184643736 2314 40-41 -4.605683727018764 2314 42-43 -3.3483745489744337 2314 44-45 -3.6682482012713287 2314 46-47 -3.620771063359985 2314 48-49 -1.9823465725849587 2314 50-51 -1.5425324760509902 2314 52-53 -2.6980753531803465 2314 54-55 -2.103893380693126 2314 56-57 -2.297061012220194 2314 58-59 -4.186269663612155 2314 60-61 -2.778133994626245 2314 62-63 -2.5644793564861104 2314 64-65 -1.9208317565020856 2314 66-67 -2.997086867270987 2314 68-69 -2.7373504122761254 2314 70-71 -2.3099331113997827 2314 72-73 -1.8788698440275589 2314 74-75 -2.108540657762557 2314 76-77 -1.3411834898251165 2314 78-79 -1.0939217066405718 2314 80-81 -0.9158402351274084 2314 82-83 -0.8704406552405501 2314 84-85 -0.7951424286693367 2314 86-87 -0.3173094645129453 2314 88-89 -0.10605215221466935 2314 90-91 0.2645586221955156 2314 92-93 0.4373111035925845 2314 94-95 1.0550646287856367 2314 96-97 2.530061666449132 2314 98-99 2.4463588400496814 2314 100-101 2.293665676737291 2315 1 -2.4411961517562624 2315 2 -2.054014059021 2315 3 -1.772512638592275 2315 4 -1.9529534559898707 2315 5 -1.9853922824187187 2315 6 -3.512870466611492 2315 7 -2.4462370946250473 2315 8 -1.6382122396225363 2315 9 -1.434558288872502 2315 10-11 -3.9406663627785257 2315 12-13 -2.3320674566334603 2315 14-15 -1.628137232713918 2315 16-17 -2.5596670373953856 2315 18-19 -1.922240487783121 2315 20-21 -1.5101906763718915 2315 22-23 -1.8426500089330844 2315 24-25 -3.693574623306489 2315 26-27 -4.9867927557192075 2315 28-29 -6.439213695229832 2315 30-31 -9.132292837944426 2315 32-33 -7.267810568876229 2315 34-35 -6.665997329666759 2315 36-37 -4.5837257372360085 2315 38-39 -6.696814090528754 2315 40-41 -4.626918708296653 2315 42-43 -4.5557973420785345 2315 44-45 -3.7023409663213513 2315 46-47 -2.7209344680196637 2315 48-49 -3.5045402319865637 2315 50-51 -3.3599835479526554 2315 52-53 -2.3984860317872148 2315 54-55 -3.7797823249152387 2315 56-57 -4.020186523612171 2315 58-59 -2.920880953541861 2315 60-61 -3.1845548306465403 2315 62-63 -3.529365813923647 2315 64-65 -2.9999563096172253 2315 66-67 -3.3169293174098904 2315 68-69 -2.4763433708675713 2315 70-71 -3.1046761724201097 2315 72-73 -2.212561260427332 2315 74-75 -2.5834391618795713 2315 76-77 -1.8980036279245276 2315 78-79 -1.827349195622194 2315 80-81 -1.8389622954386553 2315 82-83 -1.492959538872114 2315 84-85 -1.32865402771154 2315 86-87 -1.9043362902450056 2315 88-89 -2.2302090953276164 2315 90-91 -1.9374515922008655 2315 92-93 -1.3613596685225247 2315 94-95 -0.6097266831587547 2315 96-97 0.8693615906457417 2315 98-99 0.8678818661819871 2315 100-101 0.8128979276485158 2316 1 -3.631426563342 2316 2 -2.2188978080554413 2316 3 -2.352764345571579 2316 4 -2.908427874571732 2316 5 -2.3050565125265514 2316 6 -5.38381001153677 2316 7 -3.2133601669754945 2316 8 -4.382220460558976 2316 9 -6.216070041349482 2316 10-11 -5.429912413577185 2316 12-13 -3.312782591550558 2316 14-15 -4.526088571759768 2316 16-17 -3.854685299338527 2316 18-19 -2.3252296816773566 2316 20-21 -3.063791602492884 2316 22-23 -3.7347304170076967 2316 24-25 -2.5858352828431137 2316 26-27 -3.396610275099814 2316 28-29 -3.395221519327265 2316 30-31 -3.0436889355308914 2316 32-33 -2.9450945161668614 2316 34-35 -4.921418958862354 2316 36-37 -4.928973528900428 2316 38-39 -5.002740117615055 2316 40-41 -4.129047533179602 2316 42-43 -3.4122160292763084 2316 44-45 -2.7293230587570747 2316 46-47 -2.961118010142574 2316 48-49 -3.5925385670591297 2316 50-51 -4.826848613708716 2316 52-53 -2.676417761597012 2316 54-55 -2.787943653852313 2316 56-57 -2.065803820566959 2316 58-59 -2.663030114382508 2316 60-61 -2.8832627767352257 2316 62-63 -1.9662621858679472 2316 64-65 -2.3906315524157407 2316 66-67 -3.046669298041536 2316 68-69 -2.2901734143565484 2316 70-71 -1.6651544164973267 2316 72-73 -1.7009229971450672 2316 74-75 -2.8919764876092735 2316 76-77 -3.1225695954686508 2316 78-79 -3.2365944217142797 2316 80-81 -3.416998317968659 2316 82-83 -2.5035565080346203 2316 84-85 -2.188831866294418 2316 86-87 -1.9647095067149678 2316 88-89 -1.6867931933517468 2316 90-91 -1.0746477572336346 2316 92-93 -1.0858245890975624 2316 94-95 -1.1414550231795602 2316 96-97 0.5212118116834361 2316 98-99 1.0212303381885075 2316 100-101 0.5541261321234892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18829.0 3 5955.0 4 5667.0 5 6507.0 6 7207.0 7 8628.0 8 10906.0 9 12922.0 10 15599.0 11 18237.0 12 21656.0 13 24456.0 14 27450.0 15 30385.0 16 34143.0 17 36517.0 18 32892.0 19 31017.0 20 32977.0 21 36378.0 22 40118.0 23 44803.0 24 49918.0 25 55609.0 26 61974.0 27 70363.0 28 78653.0 29 88263.0 30 99797.0 31 113273.0 32 128856.0 33 147727.0 34 168256.0 35 184927.0 36 181813.0 37 134585.0 38 31466.0 39 284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43209554423591 19.278419311093547 14.247493266006916 22.041991878663627 2 15.482313885603084 20.477973442210097 41.178740412866794 22.86097225932003 3 19.185152075592487 26.098975668000605 29.720082068680288 24.995790187726623 4 12.545627946941574 17.1382647854586 37.620758109739896 32.69534915785993 5 13.420686035971565 37.984017938075475 33.775296980105914 14.819999045847048 6 30.51411462013302 36.819094990718426 18.178331168189903 14.488459220958653 7 28.087526700825276 33.62684162337201 20.719267868977138 17.56636380682558 8 26.320688774962488 35.09054604357566 19.759529938284125 18.829235243177735 9 27.406911169037397 16.75329366289287 20.220997849474678 35.61879731859505 10-11 24.932169735857393 27.183683615743732 27.128837303508135 20.75530934489074 12-13 26.112349268103692 24.809421635159268 27.72870477327413 21.349524323462905 14-15 23.178617908829995 25.671547729909623 26.61714838025159 24.532685981008793 16-17 21.779914176686106 29.17720732396476 26.968100243767985 22.07477825558115 18-19 21.60315182007923 27.212601363377388 30.21222726010389 20.97201955643949 20-21 22.847612181059116 26.22773938152479 30.18642369272751 20.738224744688587 22-23 22.359362576238855 26.405861025502826 29.91014491439025 21.324631483868075 24-25 21.83036112913677 26.61534964762823 30.284681246678385 21.269607976556618 26-27 21.688369082491462 26.839814850406967 30.154233230517548 21.31758283658402 28-29 21.434666592810693 26.94103523789423 30.419699297954413 21.204598871340668 30-31 21.608767280280784 26.778048372848172 30.298283313038706 21.314901033832342 32-33 21.427370789585904 26.901571684184738 30.113703922835477 21.557353603393878 34-35 21.92572977599683 26.87491392251033 29.954966405611692 21.244389895881145 36-37 21.900160953732005 26.641016853364086 29.92803859834693 21.530783594556976 38-39 21.56757859912566 26.96190793313211 30.00514386716172 21.465369600580505 40-41 21.759039267354964 26.70616417448435 30.097024584191434 21.43777197396926 42-43 21.643938213230182 26.72331018759954 29.883892636191117 21.748858962979156 44-45 21.611383434530715 27.017660353436202 29.62426369446545 21.746692517567627 46-47 21.71212142915121 27.18595868009301 29.114658683435273 21.987261207320508 48-49 21.807253253563015 27.246047300588533 29.002388716015 21.94431072983345 50-51 21.74146146218818 27.408720704748184 28.819798021095828 22.030019811967804 52-53 21.837717011224473 27.64732733513166 28.539915542352194 21.975040111291666 54-55 21.523884044637022 27.46388665764296 28.71442735162772 22.297801946092303 56-57 21.56496250533063 27.704675814254898 28.647450777949324 22.08291090246515 58-59 21.644426298787344 27.25539773609052 28.730878416934676 22.369297548187465 60-61 21.705898219959277 27.426496038651493 28.795745280158886 22.071860461230344 62-63 21.49462889669332 27.384105849616745 28.76634805551405 22.354917198175883 64-65 21.417330426742797 27.159620793991817 28.805723507631708 22.61732527163368 66-67 21.463972031903943 27.459886788301187 28.442512212415483 22.633628967379384 68-69 21.455000397380676 27.221268482079804 28.58147986473305 22.742251255806472 70-71 21.356232206719962 27.36761608713003 28.53614888022867 22.740002825921334 72-73 21.615067205129048 27.209445917623277 28.31139323560212 22.864093641645546 74-75 21.472485665189645 27.357136648496027 28.16266081589261 23.00771687042172 76-77 21.565797031529694 27.289482577642016 28.243338615549778 22.90138177527851 78-79 21.636725278981835 27.379635239413684 28.10702146576241 22.876618015842066 80-81 21.541918541558402 27.54727191789197 27.959641426955645 22.951168113593983 82-83 21.745616781427863 27.476130124939374 27.75245970581682 23.025793387815945 84-85 21.742315526063816 27.069166831821473 27.82184293658772 23.366674705526997 86-87 21.711905274087776 27.016935499081875 27.96352133656096 23.30763789026939 88-89 21.907521282425034 27.0066840722343 27.7995804995565 23.286214145784164 90-91 21.90337047478131 27.129691995865553 27.78608015857799 23.180857370775147 92-93 22.033933515525103 26.80126889523257 27.77232953548262 23.392468053759703 94-95 21.901437442271636 26.76587906152184 27.703836649858776 23.628846846347745 96-97 22.271841258993362 26.578838622168078 27.53055999841284 23.618760120425726 98-99 22.195447782395405 26.50194054290281 27.322576459875336 23.98003521482645 100-101 22.28094139080871 26.496271419087908 27.48398810715793 23.73879908294545 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3331.0 1 2012.5 2 722.0 3 754.5 4 687.0 5 653.5 6 962.0 7 1842.5 8 2985.0 9 3439.5 10 3078.0 11 2633.5 12 2551.0 13 2800.5 14 3094.5 15 3283.5 16 3478.0 17 3713.0 18 4084.5 19 4492.5 20 4943.0 21 5655.5 22 6543.5 23 7648.5 24 8923.0 25 10677.0 26 13304.5 27 16511.5 28 19248.5 29 22161.5 30 25496.5 31 29223.5 32 33734.5 33 37983.0 34 42798.5 35 47483.0 36 51948.5 37 56717.5 38 60344.0 39 61729.0 40 62845.5 41 66150.0 42 69570.0 43 72766.0 44 75637.0 45 77230.0 46 77353.5 47 78221.5 48 80692.0 49 80574.0 50 78106.5 51 74960.0 52 71873.0 53 69404.0 54 65884.0 55 60364.0 56 53708.5 57 47477.0 58 42369.0 59 37323.5 60 31596.5 61 25780.5 62 20488.5 63 15917.0 64 12256.5 65 9500.5 66 7213.5 67 5480.0 68 4069.5 69 2929.5 70 2185.0 71 1714.0 72 1385.5 73 1085.5 74 880.5 75 693.5 76 449.0 77 287.5 78 189.5 79 117.0 80 81.5 81 62.5 82 45.0 83 27.0 84 17.0 85 10.5 86 5.5 87 7.0 88 5.5 89 3.0 90 2.0 91 3.5 92 3.0 93 0.5 94 1.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12196208408428151 2 0.11686445010107131 3 0.12958471433954913 4 0.14216205426074063 5 0.13877951208496564 6 0.1393035679150153 7 0.14787902695219135 8 0.14854600709952726 9 0.15464411130374134 10-11 0.15038020250470102 12-13 0.15035638178515331 14-15 0.1374455517902938 16-17 0.14113776332018907 18-19 0.15888419938323392 20-21 0.1893032582456612 22-23 0.20783577805378053 24-25 0.2275355131197377 26-27 0.22936970852491145 28-29 0.2309180552955127 30-31 0.23168031832103944 32-33 0.2329666371766159 34-35 0.20216644680142526 36-37 0.1906133978207853 38-39 0.204167387243433 40-41 0.19192353739590942 42-43 0.19525843813258897 44-45 0.1991173946993182 46-47 0.2202463729381381 48-49 0.23825483691620775 50-51 0.2538812289395063 52-53 0.275105490056517 54-55 0.26862625433953957 56-57 0.23734964957339474 58-59 0.2309656967346081 60-61 0.209527049141668 62-63 0.199712912688011 64-65 0.19075632213807156 66-67 0.18634948902174497 68-69 0.19268580042143618 70-71 0.19633037051223598 72-73 0.23353833444576094 74-75 0.24578218429328452 76-77 0.2625281501353255 78-79 0.2517135434606646 80-81 0.25635858377246834 82-83 0.25333335238990895 84-85 0.23906474138082995 86-87 0.2287980112557664 88-89 0.2313230075278238 90-91 0.2335145137262132 92-93 0.24132770973786252 94-95 0.2455916185369028 96-97 8.748945337642025 98-99 0.30626299122492334 100-101 0.3173872672537045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2099013.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.048292170381465 #Duplication Level Percentage of deduplicated Percentage of total 1 78.43014938906893 44.7430607731427 2 11.887885904553901 13.563671767423008 3 4.120619264688767 7.052228752046016 4 1.8448468061335648 4.2098143842361075 5 1.0204962483148199 2.9108784066320994 6 0.5972247690363754 2.044239186922544 7 0.3902282909399766 1.5583300288284685 8 0.2941502062152372 1.3424613524915847 9 0.19152047196896219 0.9833324257345236 >10 1.0851553014124886 11.485272693452652 >50 0.08945515308043767 3.524941136248009 >100 0.04613729746809804 4.468402545895334 >500 0.0013637741558368843 0.5041990181145136 >1k 5.114153084388257E-4 0.44288211563187846 >5k 1.7047176947960856E-4 0.6484225968325757 >10k+ 8.523588473980428E-5 0.5178628163679738 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10650 0.5073813263662493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7845 0.3737470897035893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5490 0.26155150063386934 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2165 0.10314371564158964 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.764143909542247E-5 0.0 0.0 0.0 0.0 12-13 4.764143909542247E-5 0.0 0.0 4.764143909542247E-5 0.0 14-15 4.764143909542247E-5 0.0 0.0 4.764143909542247E-5 0.0 16-17 4.764143909542247E-5 0.0 0.0 4.764143909542247E-5 0.0 18-19 4.764143909542247E-5 2.3820719547711235E-5 0.0 4.764143909542247E-5 0.0 20-21 4.764143909542247E-5 4.764143909542247E-5 0.0 2.6202791502482356E-4 0.0 22-23 4.764143909542247E-5 4.764143909542247E-5 0.0 5.716972691450696E-4 0.0 24-25 4.764143909542247E-5 4.764143909542247E-5 0.0 0.0012148566969332729 0.0 26-27 4.764143909542247E-5 4.764143909542247E-5 0.0 0.0030014106630116156 0.0 28-29 4.764143909542247E-5 4.764143909542247E-5 0.0 0.00907569414767798 0.0 30-31 4.764143909542247E-5 4.764143909542247E-5 0.0 0.030371417423331822 0.0 32-33 4.764143909542247E-5 4.764143909542247E-5 0.0 0.07470177650162244 0.0 34-35 7.14621586431337E-5 4.764143909542247E-5 0.0 0.1376122968271278 0.0 36-37 9.528287819084494E-5 4.764143909542247E-5 0.0 0.24123242685967167 0.0 38-39 9.528287819084494E-5 4.764143909542247E-5 0.0 0.4009979928661709 0.0 40-41 1.1910359773855618E-4 4.764143909542247E-5 0.0 0.5812970191227973 0.0 42-43 1.429243172862674E-4 4.764143909542247E-5 0.0 0.7552120925406369 0.0 44-45 1.429243172862674E-4 4.764143909542247E-5 0.0 0.9654299425491886 0.0 46-47 1.429243172862674E-4 4.764143909542247E-5 0.0 1.2118791069898092 0.0 48-49 1.429243172862674E-4 4.764143909542247E-5 0.0 1.4794572496692493 0.0 50-51 1.9056575638168988E-4 4.764143909542247E-5 0.0 1.7732858252902672 0.0 52-53 1.9056575638168988E-4 4.764143909542247E-5 0.0 2.0449134902928185 0.0 54-55 1.9056575638168988E-4 4.764143909542247E-5 0.0 2.3290708537774663 0.0 56-57 1.9056575638168988E-4 4.764143909542247E-5 0.0 2.652508583796289 0.0 58-59 1.9056575638168988E-4 4.764143909542247E-5 0.0 3.017370545108582 0.0 60-61 1.9056575638168988E-4 4.764143909542247E-5 0.0 3.410817369878129 0.0 62-63 1.9056575638168988E-4 4.764143909542247E-5 2.3820719547711235E-5 3.791877420482865 4.764143909542247E-5 64-65 1.9056575638168988E-4 4.764143909542247E-5 4.764143909542247E-5 4.160288669007767 4.764143909542247E-5 66-67 2.858486345725348E-4 4.764143909542247E-5 4.764143909542247E-5 4.568837829970563 7.14621586431337E-5 68-69 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 5.024552015637826 9.528287819084494E-5 70-71 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 5.525668492763027 9.528287819084494E-5 72-73 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 6.008252450080109 9.528287819084494E-5 74-75 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 6.4808078844676045 9.528287819084494E-5 76-77 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 6.969751973903925 9.528287819084494E-5 78-79 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 7.511911550809833 9.528287819084494E-5 80-81 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 8.090564470062834 9.528287819084494E-5 82-83 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 8.673814788188544 1.429243172862674E-4 84-85 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 9.222263035055047 1.429243172862674E-4 86-87 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 9.694508800088423 1.429243172862674E-4 88-89 3.334900736679573E-4 4.764143909542247E-5 4.764143909542247E-5 10.179975064470778 1.429243172862674E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5385 0.0 51.966732 1 GTATCAA 10365 0.0 44.150707 1 TATCAAC 13180 0.0 34.613895 2 ATCAACG 13250 0.0 34.32536 3 TCAACGC 13365 0.0 34.06438 4 CAACGCA 13655 0.0 33.170303 5 AACGCAG 14050 0.0 32.20582 6 ACGCAGA 16160 0.0 27.826086 7 CGCAGAG 16180 0.0 27.646338 8 GTACATG 12145 0.0 27.417627 1 TACATGG 12020 0.0 27.115831 2 GCAGAGT 16595 0.0 25.31103 9 ACATGGG 12510 0.0 24.851274 3 GAGTACT 9065 0.0 23.582298 12-13 TGGTATC 2465 0.0 23.46834 2 CATGGGG 4995 0.0 22.31552 4 GTACTTT 9730 0.0 21.880402 14-15 GTGGTAT 2835 0.0 21.732634 1 AGTACTT 9270 0.0 21.563997 12-13 CATGGGA 7170 0.0 21.449776 4 >>END_MODULE