FastQCFastQC Report
Fri 27 May 2016
SRR939888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099013
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116310.5541175781188588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97250.46331299520298347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67240.3203410364776207No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22160.10557342903545618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59550.050.5287671
GTATCAA102150.040.0033151
TATCAAC133950.030.2873632
ATCAACG134450.030.0962543
TCAACGC136550.029.6334064
CAACGCA138650.029.116155
AACGCAG142550.028.3861226
ACGCAGA164250.024.4625827
CGCAGAG164850.024.3159928
GTACATG120850.024.2701821
TACATGG121200.023.7251012
ACATGGG120900.022.8752843
GCAGAGT175050.022.3300329
GAGTACT93200.022.14101812-13
TGGTATC29150.021.9752222
AGTACTT95550.021.37306212-13
GTACTTT99550.020.6095814-15
CATGGGA72650.020.3724484
AGAGTAC164950.019.84362610-11
CAGAGTA171500.019.611310-11
Produced by FastQC (version 0.11.5)