##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939888_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099013 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.372226851382056 31.0 31.0 34.0 30.0 34.0 2 31.59947556303844 31.0 31.0 34.0 30.0 34.0 3 31.62989176341452 31.0 31.0 34.0 30.0 34.0 4 35.454537918536 37.0 35.0 37.0 33.0 37.0 5 35.19054527056288 37.0 35.0 37.0 32.0 37.0 6 35.22421490481479 37.0 35.0 37.0 32.0 37.0 7 35.13080242952283 37.0 35.0 37.0 32.0 37.0 8 35.09798081288682 37.0 35.0 37.0 32.0 37.0 9 36.66206307440687 39.0 35.0 39.0 32.0 39.0 10-11 36.52757867626356 38.0 35.0 39.0 32.0 39.0 12-13 36.439397469191476 38.0 35.0 39.0 32.0 39.0 14-15 37.43105140368354 39.0 36.0 41.0 32.0 41.0 16-17 37.357901070646065 39.0 36.0 41.0 32.0 41.0 18-19 37.37711462482605 39.0 36.0 41.0 32.0 41.0 20-21 37.317644292817626 39.0 36.0 40.5 31.5 41.0 22-23 37.18416393800324 39.0 36.0 40.0 31.0 41.0 24-25 37.029504581438985 39.0 36.0 40.0 31.0 41.0 26-27 36.7321355322716 39.0 35.5 40.0 30.0 41.0 28-29 36.4972089262906 38.0 35.0 40.0 30.0 41.0 30-31 36.210134001075744 38.0 35.0 40.0 30.0 41.0 32-33 36.15781512548993 38.0 35.0 40.0 30.0 41.0 34-35 35.9901982503205 38.0 35.0 40.0 30.0 41.0 36-37 36.005193155068596 38.0 35.0 40.0 30.0 41.0 38-39 35.89917284933443 38.0 35.0 40.0 29.0 41.0 40-41 35.798921683667515 38.0 35.0 40.0 29.0 41.0 42-43 35.54079726995498 38.0 34.0 40.0 27.5 41.0 44-45 35.23307025730665 38.0 34.0 40.0 27.0 41.0 46-47 34.88197905396488 38.0 33.5 40.0 25.5 41.0 48-49 34.52713846936632 38.0 33.0 40.0 24.0 41.0 50-51 34.286111853523536 38.0 33.0 40.0 24.0 41.0 52-53 34.03899404148521 38.0 33.0 40.0 23.5 41.0 54-55 33.76994401654492 37.0 33.0 40.0 23.0 41.0 56-57 33.40969898709537 37.0 32.0 40.0 22.0 41.0 58-59 33.051513497057904 36.5 31.5 39.5 20.5 41.0 60-61 32.75055180696832 36.0 31.0 39.0 20.0 41.0 62-63 32.46721625830807 36.0 31.0 39.0 19.0 40.0 64-65 32.0269286088271 35.5 31.0 39.0 17.5 40.0 66-67 31.128901536102923 35.0 29.5 38.0 10.5 40.0 68-69 29.273439707138543 34.0 26.0 37.0 7.5 39.5 70-71 28.623379178690175 33.5 25.0 37.0 7.0 39.0 72-73 29.15590541840379 34.0 26.5 36.0 7.0 39.0 74-75 29.234847997606494 34.0 27.5 36.0 7.0 39.0 76-77 28.767331360024926 32.5 27.0 35.0 6.5 37.5 78-79 29.28699393476839 34.0 29.0 35.0 2.0 37.5 80-81 29.088261244689768 34.0 29.0 35.0 2.0 37.0 82-83 28.63049180733993 33.5 27.5 35.0 2.0 36.5 84-85 28.267140079646957 33.0 27.0 35.0 2.0 36.0 86-87 27.869166365334564 33.0 26.5 35.0 2.0 36.0 88-89 27.405566092253835 33.0 26.0 35.0 2.0 35.0 90-91 26.904575150320653 32.0 25.0 35.0 2.0 35.0 92-93 26.403249527277822 32.0 25.0 34.0 2.0 35.0 94-95 25.600433870585842 32.0 22.5 34.0 2.0 35.0 96-97 25.058737130260745 31.0 20.0 34.0 2.0 35.0 98-99 24.378177028917875 31.0 10.0 34.0 2.0 35.0 100-101 22.906504628604015 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23030898169961844 1101 2 0.3106826565675185 1101 3 0.28452525439646337 1101 4 0.17633749967120593 1101 5 0.1559188667408833 1101 6 0.10127609347281918 1101 7 0.1103846030097273 1101 8 0.267522024682755 1101 9 0.4070632337287208 1101 10-11 0.30025803378092064 1101 12-13 0.2899192466083136 1101 14-15 0.389230764351602 1101 16-17 0.3598611597174397 1101 18-19 0.28201822522297704 1101 20-21 0.42818361668236093 1101 22-23 0.24221328752881988 1101 24-25 0.3168470706770137 1101 26-27 0.43334920393493803 1101 28-29 0.42290855672575844 1101 30-31 0.27832535966680894 1101 32-33 0.24042102334315274 1101 34-35 0.1444379078610325 1101 36-37 0.3608387864414482 1101 38-39 0.38642379652289804 1101 40-41 0.4384353981446978 1101 42-43 0.3340191639040171 1101 44-45 0.2453314043754986 1101 46-47 0.21531780962455827 1101 48-49 0.27183043262839135 1101 50-51 0.4132436952972043 1101 52-53 0.17552339609202505 1101 54-55 -0.07392262600041022 1101 56-57 -0.12911065283613965 1101 58-59 -0.11714989010404508 1101 60-61 -0.014225143937707685 1101 62-63 -0.07700774195162552 1101 64-65 -0.09010241251366224 1101 66-67 -0.29478336114124204 1101 68-69 -8.582893256472037 1101 70-71 -6.614863553181813 1101 72-73 -1.5528431760082313 1101 74-75 -0.36568498009905426 1101 76-77 -0.09994197306101782 1101 78-79 -0.24507707777059196 1101 80-81 -0.29423365375037847 1101 82-83 -0.28486532624100747 1101 84-85 -0.2256811810270456 1101 86-87 -0.3057200833353164 1101 88-89 -0.14024499409077862 1101 90-91 -0.32543360406179644 1101 92-93 -0.38388516101106873 1101 94-95 -0.21759131401360676 1101 96-97 -0.7019347645498435 1101 98-99 -0.4984176360273018 1101 100-101 -0.5227482320770385 1102 1 0.28792539490314795 1102 2 0.18613324665672337 1102 3 0.30889040425865133 1102 4 0.3061571245338399 1102 5 0.3849910187829906 1102 6 0.37501031785178185 1102 7 0.28388687533504964 1102 8 0.22309468643317132 1102 9 0.4064198884525112 1102 10-11 0.36982054905726613 1102 12-13 0.38633645924907967 1102 14-15 0.5103249878562153 1102 16-17 0.5568893764935012 1102 18-19 0.4984120041983573 1102 20-21 0.3810246691654271 1102 22-23 0.39907688740084524 1102 24-25 0.389168394567335 1102 26-27 0.42515214057014106 1102 28-29 0.2677089607167886 1102 30-31 0.21174533347594604 1102 32-33 0.21107938663302406 1102 34-35 0.08837198161275239 1102 36-37 0.212755980204129 1102 38-39 0.5176565454304551 1102 40-41 0.37612728394618955 1102 42-43 0.2363848115515097 1102 44-45 0.21763675641204827 1102 46-47 0.24986818331129257 1102 48-49 0.1339784308558336 1102 50-51 0.11317702584850053 1102 52-53 0.3925047554731478 1102 54-55 0.1687140513373322 1102 56-57 -0.016321420697295252 1102 58-59 0.11221698362540877 1102 60-61 0.19102385800773192 1102 62-63 0.17729497112730286 1102 64-65 0.1318664873311448 1102 66-67 0.6343399852875216 1102 68-69 -4.326884063838069 1102 70-71 0.3095596467927493 1102 72-73 0.6128506482745486 1102 74-75 0.4298159250554576 1102 76-77 0.3071928750896227 1102 78-79 0.258992143200377 1102 80-81 0.344546106557555 1102 82-83 0.10968789903019172 1102 84-85 0.11480110934175158 1102 86-87 -0.06645525559287435 1102 88-89 -0.18948512631686043 1102 90-91 -0.13067597383778207 1102 92-93 -0.17226232130985863 1102 94-95 0.18693972453941754 1102 96-97 -0.4059171960074899 1102 98-99 -0.25751178103881855 1102 100-101 -0.23756097140644883 1103 1 0.29009835754360225 1103 2 0.3176881165442538 1103 3 0.2332561313248469 1103 4 0.19800787842055456 1103 5 0.3335507918211178 1103 6 0.24912805444626684 1103 7 0.1945706923452306 1103 8 0.23323421843593195 1103 9 0.46020593554033695 1103 10-11 0.3732377254734587 1103 12-13 0.46071487453486526 1103 14-15 0.4085211719792028 1103 16-17 0.37917531590551334 1103 18-19 0.39080197740784683 1103 20-21 0.27685486645583524 1103 22-23 0.23038394687654318 1103 24-25 0.3247129592677709 1103 26-27 0.3578740865805159 1103 28-29 0.3704494480932681 1103 30-31 0.43109656092015314 1103 32-33 0.4208797578444816 1103 34-35 0.23480519944367018 1103 36-37 0.40362017400041594 1103 38-39 0.5005009201557158 1103 40-41 0.5014311950148524 1103 42-43 0.45777875832244774 1103 44-45 0.5340598260680949 1103 46-47 0.5076267835113697 1103 48-49 0.649002147142923 1103 50-51 0.6372561936165368 1103 52-53 0.5066203457538521 1103 54-55 0.5091545882422963 1103 56-57 0.5745040242126649 1103 58-59 0.44359198569610214 1103 60-61 0.608234125454274 1103 62-63 0.41077453709176126 1103 64-65 0.45823102312116504 1103 66-67 0.690967137213164 1103 68-69 -3.515822937349842 1103 70-71 1.091587798074741 1103 72-73 1.2996194401039602 1103 74-75 1.0290314401671665 1103 76-77 0.635557155914853 1103 78-79 0.5910578549734851 1103 80-81 0.4595383483774924 1103 82-83 0.3306469411692454 1103 84-85 0.4620986866240493 1103 86-87 0.3683122907023204 1103 88-89 0.15933359683771187 1103 90-91 0.16816268794758216 1103 92-93 -0.002088688366487901 1103 94-95 -0.15923415904731186 1103 96-97 -0.5219024080496091 1103 98-99 -0.011299220589712888 1103 100-101 -0.18362463433819087 1104 1 0.16714750387950517 1104 2 0.23755109899821036 1104 3 0.19611749229392217 1104 4 0.13728098872714156 1104 5 0.3095333245722074 1104 6 0.24778234640477592 1104 7 0.20967206625301316 1104 8 0.186132363473817 1104 9 0.2584202286600146 1104 10-11 0.2755795477570544 1104 12-13 0.19444404094235068 1104 14-15 0.40352297593297237 1104 16-17 0.32294068579897584 1104 18-19 0.17384336052201377 1104 20-21 0.32076247914073974 1104 22-23 0.30583172137817627 1104 24-25 0.2942416918969428 1104 26-27 0.19359817703531235 1104 28-29 0.35227202198279883 1104 30-31 0.3112176124385826 1104 32-33 0.31122632431913644 1104 34-35 0.27385872503285213 1104 36-37 0.28471162823597496 1104 38-39 0.2617756312120818 1104 40-41 0.1907208934020801 1104 42-43 0.2768038353533484 1104 44-45 0.20906096649863315 1104 46-47 0.17306904247619315 1104 48-49 0.3170497715224272 1104 50-51 0.15645586019611812 1104 52-53 0.28903656557909585 1104 54-55 0.320394671680333 1104 56-57 0.021353740349141503 1104 58-59 -0.05988736421701901 1104 60-61 0.10530425571217705 1104 62-63 0.13173606518575554 1104 64-65 0.2132312926774702 1104 66-67 0.16140821427724106 1104 68-69 -3.8481537285598186 1104 70-71 0.646983693354187 1104 72-73 0.7833942237331826 1104 74-75 0.4733649518223686 1104 76-77 0.2778362337834501 1104 78-79 -0.0753154100169553 1104 80-81 -0.001964863489622104 1104 82-83 -0.061059847917455556 1104 84-85 -0.10550603600250597 1104 86-87 -0.07690453624667626 1104 88-89 -0.13763555347932765 1104 90-91 -0.2235409908739996 1104 92-93 -0.2728455610156466 1104 94-95 -0.054239902670229156 1104 96-97 -0.3523069083958852 1104 98-99 -0.027214447203629533 1104 100-101 0.01919160705155676 1105 1 0.24274275078635554 1105 2 0.23645115332260858 1105 3 0.16958130286999662 1105 4 0.18327123366243825 1105 5 0.19592916774651314 1105 6 0.23534851921736788 1105 7 0.17146667337906507 1105 8 0.1780092736313108 1105 9 0.3225499325314871 1105 10-11 0.16670421529488522 1105 12-13 0.19625870458191486 1105 14-15 0.291400852355963 1105 16-17 0.4263060844328308 1105 18-19 0.33124987074698 1105 20-21 0.22057804085994093 1105 22-23 0.2967987763902613 1105 24-25 0.3030454067705648 1105 26-27 0.4062649576441473 1105 28-29 0.3461197055250196 1105 30-31 0.3892129345293256 1105 32-33 0.37676505321796583 1105 34-35 0.3718473092834458 1105 36-37 0.21742799163255455 1105 38-39 0.3472934916472852 1105 40-41 0.4403234415796575 1105 42-43 0.46213021462556725 1105 44-45 0.4041058502933197 1105 46-47 0.40229007279153706 1105 48-49 0.49397527083810644 1105 50-51 0.5548422102829846 1105 52-53 0.5507765342943358 1105 54-55 0.6386030790872965 1105 56-57 0.5027428120389459 1105 58-59 0.5567674372118319 1105 60-61 0.5965452878129156 1105 62-63 0.5564557293165322 1105 64-65 0.5037746308511046 1105 66-67 1.1021720798954568 1105 68-69 2.4054412814908694 1105 70-71 2.709618242683746 1105 72-73 1.8412555381682658 1105 74-75 1.3211997814317193 1105 76-77 1.0459368895789574 1105 78-79 0.9100218251628256 1105 80-81 0.9580546681721707 1105 82-83 0.9343699047158864 1105 84-85 0.6495544094216648 1105 86-87 0.6557621232425284 1105 88-89 0.8290301935753348 1105 90-91 0.7362940576478678 1105 92-93 0.7379156938016642 1105 94-95 0.9128658002251129 1105 96-97 0.38704017156684856 1105 98-99 0.4572099048399956 1105 100-101 0.5663080402489236 1106 1 0.503465774715707 1106 2 0.46156109628889297 1106 3 0.4092184992415895 1106 4 0.18579091055065788 1106 5 0.34803283098879234 1106 6 0.4268092230295508 1106 7 0.3060314549783101 1106 8 0.4286041617205427 1106 9 0.47532740591599065 1106 10-11 0.3530105046713885 1106 12-13 0.5303575546281536 1106 14-15 0.7446341151685019 1106 16-17 0.6621911608671525 1106 18-19 0.6331735870453983 1106 20-21 0.49280934565947376 1106 22-23 0.7290265326118117 1106 24-25 0.6881662566013631 1106 26-27 0.6707925607779046 1106 28-29 0.47851523814046004 1106 30-31 0.8160507924938685 1106 32-33 0.8772256455833443 1106 34-35 0.8221021680607663 1106 36-37 0.9779565665450818 1106 38-39 0.9815411861207792 1106 40-41 0.8253037005745583 1106 42-43 0.6907464184305141 1106 44-45 0.8704716494624662 1106 46-47 0.9052384001802665 1106 48-49 0.8586183543052215 1106 50-51 0.9376542694794168 1106 52-53 0.8691639504119735 1106 54-55 1.028360395522995 1106 56-57 1.0320307043002046 1106 58-59 1.2329575980915806 1106 60-61 1.1306752870062056 1106 62-63 1.0196691928358916 1106 64-65 1.1254892235270475 1106 66-67 1.7535840968447012 1106 68-69 3.0894595307875683 1106 70-71 3.174611474272524 1106 72-73 2.1487854014803993 1106 74-75 1.7041238703075088 1106 76-77 1.2788894929808379 1106 78-79 1.2102129867322375 1106 80-81 1.332823439380288 1106 82-83 1.1931588137543159 1106 84-85 1.1663670216553506 1106 86-87 1.2518477992512977 1106 88-89 1.4506256454268183 1106 90-91 1.2765862720864298 1106 92-93 1.2365313520302372 1106 94-95 1.6684325445004085 1106 96-97 1.5114047732657845 1106 98-99 1.8444714424980546 1106 100-101 1.8618444327580157 1107 1 0.1372762617595491 1107 2 0.11039553331996999 1107 3 0.1332229906429916 1107 4 0.08963444622319372 1107 5 0.01009433438987628 1107 6 0.11829789737848984 1107 7 0.1303628328998343 1107 8 0.23728255347393912 1107 9 0.05472118671880111 1107 10-11 0.05212595427073552 1107 12-13 0.05256696229574942 1107 14-15 0.17487051831837874 1107 16-17 0.10296576608118357 1107 18-19 0.101701587001088 1107 20-21 0.09833547235187723 1107 22-23 0.15044685292645 1107 24-25 0.22664580277229618 1107 26-27 0.07702348028296768 1107 28-29 0.15331299354738093 1107 30-31 0.15686898719233966 1107 32-33 0.19796614069897345 1107 34-35 0.17419790155248904 1107 36-37 0.05291248093893586 1107 38-39 0.10942590312603073 1107 40-41 -0.038018805767727315 1107 42-43 0.013366637951683913 1107 44-45 0.10397917920553112 1107 46-47 0.03668884777435011 1107 48-49 0.20982856822829632 1107 50-51 0.16353940830350666 1107 52-53 0.18539492991315143 1107 54-55 0.14435053677692622 1107 56-57 0.08998487373015962 1107 58-59 0.2948927404462083 1107 60-61 0.2749497886115648 1107 62-63 0.3715184807512415 1107 64-65 0.3309221485637508 1107 66-67 0.7559810569800511 1107 68-69 2.2435161680422873 1107 70-71 2.2333754758656497 1107 72-73 1.3493022218524935 1107 74-75 0.8345613065904836 1107 76-77 0.4791954039364512 1107 78-79 0.43841847526905653 1107 80-81 0.4281819553902544 1107 82-83 0.5078856214487715 1107 84-85 0.4224925095275971 1107 86-87 0.4280818847051471 1107 88-89 0.3697483453257959 1107 90-91 0.20255175955254856 1107 92-93 0.12797011422733462 1107 94-95 0.5855863334626932 1107 96-97 0.3913084748875697 1107 98-99 0.636684131491581 1107 100-101 0.716590609967156 1108 1 -0.22335626476634118 1108 2 -0.12619294889884358 1108 3 -0.1454254618173394 1108 4 -0.08403137630106983 1108 5 0.018901006071033066 1108 6 0.03512107527355823 1108 7 -0.027438586704633394 1108 8 -0.08385702782705096 1108 9 -0.12096597914149498 1108 10-11 -0.09804971712904376 1108 12-13 -0.1235288079048189 1108 14-15 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62-63 -0.5874506123342584 2316 64-65 -0.3800396339267884 2316 66-67 0.24491113111348994 2316 68-69 1.5326375480647911 2316 70-71 1.6645949401320976 2316 72-73 0.5686675923398816 2316 74-75 0.15668471847546428 2316 76-77 -0.2862917678445491 2316 78-79 -0.49656931043752195 2316 80-81 -0.3813485970400201 2316 82-83 -0.1846099089675164 2316 84-85 -0.2716893896858572 2316 86-87 -0.19591823859370905 2316 88-89 -0.31518100185811093 2316 90-91 -0.3123179546509647 2316 92-93 -0.3670237593605101 2316 94-95 0.06617286357586849 2316 96-97 -0.04661568603799182 2316 98-99 -0.24137297386215906 2316 100-101 -0.18376592923931057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 40.0 5 95.0 6 261.0 7 634.0 8 1432.0 9 2432.0 10 3933.0 11 5999.0 12 8257.0 13 10897.0 14 13558.0 15 17134.0 16 21328.0 17 24898.0 18 22376.0 19 17313.0 20 17027.0 21 17516.0 22 18860.0 23 21345.0 24 25244.0 25 30782.0 26 38091.0 27 46789.0 28 57650.0 29 68929.0 30 82110.0 31 98179.0 32 116896.0 33 140586.0 34 174724.0 35 218839.0 36 274193.0 37 305623.0 38 180659.0 39 14371.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.25457688858306 20.2058348955767 15.146501416741085 22.39308679909916 2 15.610041776404978 21.37267917465034 39.98040554610724 23.036873502837434 3 18.923093341057605 26.15216111198983 30.382055938698777 24.542689608253788 4 12.830125397031845 17.87058965332754 37.27871147058165 32.02057347905897 5 13.164425375164424 38.3334929321543 33.98130454647018 14.5207771462111 6 30.612864236667427 37.29433786260495 17.66296826174969 14.429829638977939 7 27.716169456787547 33.77506475662609 20.728409018905552 17.78035676768081 8 26.14552649268966 35.63522474610686 19.57515270272266 18.64409605848082 9 26.714174709732625 16.658734367057278 20.19172820749562 36.43536271571448 10-11 24.746535633652577 27.391493049352245 27.137397433936805 20.72457388305837 12-13 25.735381343517165 24.904443183534354 27.833486500560024 21.526688972388452 14-15 22.6288974865806 25.871564397171433 26.656695313463995 24.842842802783977 16-17 21.44482743013952 29.380373081531484 27.09488543381269 22.079914054516305 18-19 21.33002511180255 27.35068815676701 30.229565038425203 21.089721693005238 20-21 22.474110450959568 26.396453952405253 30.182566758757567 20.94686883787761 22-23 22.043836793769263 26.555695462581696 29.948980782872713 21.45148696077633 24-25 21.655299008747217 26.785001790129204 30.243417076760693 21.316282124362885 26-27 21.475156437253943 27.04639270663784 30.134832964865012 21.343617891243206 28-29 21.25675764198641 27.055508244948516 30.34657964161719 21.341154471447883 30-31 21.430339272574557 26.900525034355553 30.258305659513518 21.410830033556365 32-33 21.317094505329067 26.986590031925317 30.04546058740491 21.650854875340706 34-35 21.80624274922996 26.982369462317436 29.913431463398947 21.297956325053658 36-37 21.770973021538133 26.708448342626273 29.920975024464312 21.59960361137128 38-39 21.322276098505142 27.045186695745077 30.070500124339056 21.562037081410725 40-41 21.540029781952708 26.822403944856894 30.11069873134604 21.526867541844357 42-43 21.51420537731737 26.783266774191393 29.897271281836556 21.80525656665468 44-45 21.471668268541837 27.109044900111893 29.622943780023235 21.79634305132303 46-47 21.48659899406625 27.283449586010768 29.140702932924402 22.089248486998585 48-49 21.627182621615976 27.314039064742644 29.035579033099456 22.02319928054192 50-51 21.568681679584596 27.462968186102138 28.847490367281875 22.12085976703139 52-53 21.76625741790691 27.603898371546702 28.598824576856014 22.031019633690377 54-55 21.448201201185455 27.416564613414018 28.742311445393447 22.392922740007084 56-57 21.480136318123915 27.666135926383255 28.710801509964845 22.142926245527985 58-59 21.53923159921772 27.234541942040813 28.762604911385925 22.46362154735554 60-61 21.585000753993125 27.437512075205873 28.819975743576588 22.157511427224417 62-63 21.331086516502502 27.400207578051994 28.795320751444937 22.473385154000567 64-65 21.190110467071893 27.16422269506114 28.81484449517987 22.830822342687096 66-67 21.045043884447562 27.464411728920794 28.71555386968025 22.774990516951394 68-69 20.984224718004697 27.24549867044494 28.96514264913188 22.805133962418484 70-71 20.9578856496505 27.40438720045149 28.777796643000116 22.859930506897896 72-73 21.137292558745987 27.316455176653182 28.504499611509992 23.041752653090832 74-75 21.267538346984363 27.491073715562177 28.026424422933733 23.21496351451973 76-77 21.1627170524543 27.567118183904217 28.13586930233212 23.134295461309364 78-79 21.29795545383036 27.519854438108325 27.976593464865662 23.20559664319565 80-81 21.11481979784428 27.84207632674218 27.79479136493223 23.248312510481302 82-83 21.27504689108643 27.765025752579902 27.52793813092153 23.431989225412135 84-85 21.230248909241457 27.408244314668234 27.633709036406895 23.727797739683414 86-87 21.223582748506598 27.40898153796826 27.775944879985953 23.591490833539186 88-89 21.330082586749434 27.362012000924246 27.69052479627252 23.617380616053797 90-91 21.250367437049754 27.487840632606304 27.646416220303106 23.615375710040833 92-93 21.243989869249283 27.21298112013782 27.607177091849472 23.935851918763422 94-95 20.96324020215505 27.071421760274628 27.82511681129017 24.140221226280158 96-97 21.30932572469776 27.056089862712923 27.57918355833319 24.055400854256128 98-99 21.212685069215045 27.02883128387163 27.405241631427586 24.35324201548574 100-101 21.043922364411564 26.988089177391615 27.592504403099767 24.375484055097054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1238.0 1 863.5 2 542.0 3 634.0 4 600.0 5 607.0 6 996.0 7 2109.0 8 3329.5 9 3882.0 10 3517.5 11 2948.5 12 2964.5 13 3138.5 14 3311.5 15 3531.5 16 3733.0 17 3914.5 18 4203.5 19 4515.0 20 4940.5 21 5573.5 22 6393.5 23 7443.0 24 8717.5 25 10535.5 26 13196.0 27 16306.5 28 18860.0 29 21874.0 30 25623.5 31 29448.5 32 33573.5 33 37856.0 34 42971.5 35 47286.0 36 51918.5 37 57099.5 38 59967.0 39 61375.0 40 62868.0 41 65858.5 42 69294.5 43 72885.0 44 76166.5 45 77514.5 46 77587.5 47 78402.5 48 81324.5 49 81841.0 50 79050.0 51 75480.5 52 72179.0 53 70005.5 54 67263.0 55 61331.0 56 53608.0 57 47253.0 58 42442.0 59 37511.5 60 31666.5 61 25455.5 62 19985.0 63 15391.5 64 11564.5 65 8820.5 66 6512.5 67 5060.5 68 3719.0 69 2474.5 70 1884.5 71 1499.0 72 1157.0 73 911.5 74 726.5 75 587.0 76 380.5 77 192.0 78 98.0 79 58.0 80 31.0 81 22.0 82 15.0 83 7.5 84 3.0 85 1.5 86 2.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025488169916051018 2 0.021915061983894334 3 4.7641439095422464E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.811315127633797E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1910359773855616E-4 26-27 4.049522323110909E-4 28-29 2.3820719547711232E-5 30-31 3.573107932156685E-4 32-33 0.0024773548329619683 34-35 0.004668861031351402 36-37 0.09914183475757415 38-39 0.09168594953914054 40-41 0.100309050015412 42-43 0.10152390671234529 44-45 0.09487792595853384 46-47 0.08847015240019952 48-49 0.08951826406029882 50-51 0.08877982175431977 52-53 0.07005673618981874 54-55 0.059647081747468925 56-57 0.032682027219459814 58-59 0.013220499348979734 60-61 0.008932769830391712 62-63 0.0020724026006508772 64-65 0.005097633983210204 66-67 0.15092807905429836 68-69 0.4032371405036558 70-71 0.3886588601404565 72-73 0.177273794874067 74-75 0.02741764819941563 76-77 0.030657266057904357 78-79 0.005335841178687316 80-81 0.0017627332465306312 82-83 0.0 84-85 7.14621586431337E-4 86-87 0.0014054224533149627 88-89 3.811315127633797E-4 90-91 6.669801473359145E-4 92-93 0.0027155620284390804 94-95 0.07684564126091645 96-97 0.016436296487920752 98-99 0.01774643606304487 100-101 0.009885598612300161 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2099013.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.45721408163495 #Duplication Level Percentage of deduplicated Percentage of total 1 73.81098621451405 37.24296733002341 2 13.932806970804096 14.06021248167916 3 5.058316600535863 7.6568569081777795 4 2.3720265562889398 4.787434070319774 5 1.298018290783493 3.27471933899703 6 0.7736449853669819 2.3421582389907085 7 0.5213961451572965 1.8415737842278648 8 0.35008312806585556 1.4131375471349839 9 0.27244249340822313 1.237202029334982 >10 1.41481493075835 13.344829570184856 >50 0.12506906236240456 4.371089209049551 >100 0.06755730171186118 5.972092116560829 >500 0.001986124169726556 0.6644684798667437 >1k 5.674640484933006E-4 0.451250642460494 >5k 1.8915468283110017E-4 0.784964236234419 >10k+ 9.457734141555009E-5 0.5550440167574033 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11631 0.5541175781188588 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9725 0.46331299520298347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6724 0.3203410364776207 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2216 0.10557342903545618 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.764143909542247E-5 0.0 14-15 0.0 0.0 0.0 4.764143909542247E-5 0.0 16-17 0.0 0.0 0.0 4.764143909542247E-5 0.0 18-19 0.0 0.0 0.0 4.764143909542247E-5 0.0 20-21 0.0 0.0 0.0 2.3820719547711235E-4 0.0 22-23 0.0 0.0 0.0 5.955179886927808E-4 0.0 24-25 0.0 0.0 0.0 0.0012624981360286954 0.0 26-27 0.0 0.0 0.0 0.0032157971389410165 0.0 28-29 0.0 0.0 0.0 0.00962357069727534 0.0 30-31 0.0 0.0 0.0 0.032253254267601006 0.0 32-33 0.0 0.0 0.0 0.07932299609387841 0.0 34-35 0.0 0.0 0.0 0.14571134147334963 0.0 36-37 0.0 0.0 0.0 0.25516754779508277 0.0 38-39 0.0 0.0 0.0 0.42288923413051754 0.0 40-41 0.0 0.0 0.0 0.613240604036278 0.0 42-43 0.0 0.0 0.0 0.7970650967859656 0.0 44-45 0.0 0.0 0.0 1.0172638282850084 0.0 46-47 0.0 0.0 0.0 1.2738606192529538 0.0 48-49 0.0 0.0 0.0 1.5528012451566522 0.0 50-51 0.0 0.0 0.0 1.8606364038717245 0.0 52-53 0.0 0.0 0.0 2.144484098002252 0.0 54-55 0.0 0.0 0.0 2.4407185662975888 0.0 56-57 0.0 0.0 0.0 2.7734701976595666 0.0 58-59 0.0 0.0 0.0 3.1317099989375956 0.0 60-61 7.14621586431337E-5 0.0 0.0 3.5060526066298783 2.3820719547711235E-5 62-63 9.528287819084494E-5 0.0 0.0 3.8642447664688118 4.764143909542247E-5 64-65 9.528287819084494E-5 0.0 0.0 4.216934340092225 4.764143909542247E-5 66-67 1.429243172862674E-4 0.0 0.0 4.589442752379332 4.764143909542247E-5 68-69 1.429243172862674E-4 0.0 0.0 5.013951795439095 4.764143909542247E-5 70-71 1.429243172862674E-4 0.0 0.0 5.512900587085454 4.764143909542247E-5 72-73 1.429243172862674E-4 0.0 0.0 6.004202927756999 4.764143909542247E-5 74-75 1.429243172862674E-4 0.0 0.0 6.4972441809555255 4.764143909542247E-5 76-77 1.429243172862674E-4 0.0 0.0 7.013367711395785 4.764143909542247E-5 78-79 1.429243172862674E-4 0.0 0.0 7.58916214430306 4.764143909542247E-5 80-81 1.429243172862674E-4 0.0 0.0 8.20945368132546 4.764143909542247E-5 82-83 1.429243172862674E-4 0.0 0.0 8.834676107294237 4.764143909542247E-5 84-85 1.429243172862674E-4 0.0 0.0 9.423143163000896 4.764143909542247E-5 86-87 1.429243172862674E-4 0.0 0.0 10.005011879392839 4.764143909542247E-5 88-89 1.429243172862674E-4 0.0 0.0 10.634569676319298 4.764143909542247E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5955 0.0 50.528767 1 GTATCAA 10215 0.0 40.003315 1 TATCAAC 13395 0.0 30.287363 2 ATCAACG 13445 0.0 30.096254 3 TCAACGC 13655 0.0 29.633406 4 CAACGCA 13865 0.0 29.11615 5 AACGCAG 14255 0.0 28.386122 6 ACGCAGA 16425 0.0 24.462582 7 CGCAGAG 16485 0.0 24.315992 8 GTACATG 12085 0.0 24.270182 1 TACATGG 12120 0.0 23.725101 2 ACATGGG 12090 0.0 22.875284 3 GCAGAGT 17505 0.0 22.330032 9 GAGTACT 9320 0.0 22.141018 12-13 TGGTATC 2915 0.0 21.975222 2 AGTACTT 9555 0.0 21.373062 12-13 GTACTTT 9955 0.0 20.60958 14-15 CATGGGA 7265 0.0 20.372448 4 AGAGTAC 16495 0.0 19.843626 10-11 CAGAGTA 17150 0.0 19.6113 10-11 >>END_MODULE