FastQCFastQC Report
Fri 27 May 2016
SRR939887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897353
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165530.4247241653501748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115900.29738132522252925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79570.2041642109400919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93750.046.757151
GTATCAA170550.040.667081
TATCAAC214700.032.878972
ATCAACG215050.032.806353
TCAACGC217250.032.6055874
CAACGCA221000.032.0746155
AACGCAG226100.031.266796
ACGCAGA254600.027.5431487
CGCAGAG256500.027.2651378
TACATGG188650.027.1087362
GTACATG189550.026.4043851
ACATGGG195250.025.2470663
GCAGAGT267500.024.5479629
GAGTACT141650.023.49468212-13
CATGGGG88250.021.9349884
AGTACTT149450.021.85579112-13
GTACTTT153350.021.68903214-15
AGAGTAC235600.021.05294410-11
CAGAGTA262650.020.39297710-11
CATGGGA103450.020.2255364
Produced by FastQC (version 0.11.5)