##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939887_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3897353 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.839018687811958 31.0 30.0 33.0 16.0 34.0 2 29.69366259612614 31.0 30.0 33.0 25.0 34.0 3 29.944348895263015 31.0 30.0 34.0 25.0 34.0 4 33.736094215740785 35.0 33.0 37.0 28.0 37.0 5 33.71092328562489 35.0 35.0 37.0 28.0 37.0 6 33.7413470116769 35.0 35.0 37.0 28.0 37.0 7 33.66215403121041 35.0 33.0 37.0 28.0 37.0 8 33.53221301739924 35.0 33.0 37.0 28.0 37.0 9 34.78023494407615 37.0 34.0 39.0 27.0 39.0 10-11 34.62307956194884 37.0 34.0 39.0 27.0 39.0 12-13 34.50946822112341 37.0 33.5 39.0 27.0 39.0 14-15 35.151019807546305 38.0 33.5 40.0 26.5 41.0 16-17 35.00349172887341 38.0 33.0 40.0 26.0 41.0 18-19 34.89000316368571 38.0 33.0 40.0 25.0 41.0 20-21 35.07945931507872 38.0 34.0 40.0 26.0 41.0 22-23 34.96377194983364 38.0 34.0 40.0 25.0 41.0 24-25 34.75322071159579 38.0 33.0 40.0 24.5 41.0 26-27 34.63851247243963 38.0 33.0 40.0 24.5 41.0 28-29 34.6847224257079 38.0 33.0 40.0 24.0 41.0 30-31 34.449629402314855 38.0 33.0 40.0 24.0 41.0 32-33 34.07513946003865 38.0 33.0 40.0 23.5 41.0 34-35 33.887266177839166 38.0 33.0 40.0 22.5 41.0 36-37 33.68255364602591 38.0 32.5 40.0 20.5 41.0 38-39 33.45619257480654 37.5 32.0 40.0 18.5 41.0 40-41 33.21800193618592 37.0 31.0 40.0 18.0 41.0 42-43 32.802082977857026 37.0 31.0 40.0 15.0 41.0 44-45 32.42801768277084 37.0 30.5 40.0 13.5 41.0 46-47 32.00818055228767 37.0 30.0 39.5 9.0 41.0 48-49 31.62420365822649 36.0 30.0 39.0 8.5 40.5 50-51 30.997541921401528 35.5 29.5 38.5 8.0 40.0 52-53 31.16579265465561 36.0 30.0 39.0 7.0 40.0 54-55 31.264585348055462 36.0 30.0 39.0 2.0 40.0 56-57 30.958957400061017 36.0 29.0 39.0 2.0 40.0 58-59 30.564752795038068 35.5 28.0 39.0 2.0 40.0 60-61 30.16546743392246 35.0 28.0 39.0 2.0 40.0 62-63 29.708697031036195 35.0 27.5 38.0 2.0 40.0 64-65 29.16920266139608 34.0 26.0 38.0 2.0 40.0 66-67 28.57253705271244 34.0 26.0 38.0 2.0 40.0 68-69 28.0373411133146 33.5 25.5 37.0 2.0 39.0 70-71 27.45231455811162 33.0 24.0 36.0 2.0 39.0 72-73 26.76252625820653 32.0 22.0 36.0 2.0 38.5 74-75 26.40315067688249 32.0 22.0 35.5 2.0 38.0 76-77 25.823493791812034 32.0 20.0 35.0 2.0 37.5 78-79 25.141374928060145 31.0 18.0 35.0 2.0 37.0 80-81 24.403122965766766 31.0 11.5 34.0 2.0 36.0 82-83 23.652537504300994 30.0 7.0 34.0 2.0 36.0 84-85 22.885907178538872 29.5 2.0 34.0 2.0 35.0 86-87 22.048309840037586 29.0 2.0 34.0 2.0 35.0 88-89 21.102719846008302 28.5 2.0 34.0 2.0 35.0 90-91 19.708663546771362 26.0 2.0 33.0 2.0 34.5 92-93 18.163877508657798 23.5 2.0 32.0 2.0 34.0 94-95 17.60385741296721 21.0 2.0 32.0 2.0 34.0 96-97 17.191024651859863 20.5 2.0 32.0 2.0 34.0 98-99 16.24772711119573 10.0 2.0 31.5 2.0 34.0 100-101 14.616746417376103 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7300028922084678 1101 2 0.9314178024340976 1101 3 0.88401718316263 1101 4 0.8345857971793862 1101 5 0.857223795086604 1101 6 0.921039869072807 1101 7 0.9180458178366564 1101 8 1.0161355700069308 1101 9 0.9637069670237324 1101 10-11 1.1237470565294245 1101 12-13 1.0982530014139655 1101 14-15 1.3398663559791544 1101 16-17 1.0658825520185218 1101 18-19 1.1564616716710177 1101 20-21 1.1105077948567654 1101 22-23 1.2167757894835134 1101 24-25 1.3363870772863677 1101 26-27 1.3901512556758817 1101 28-29 1.257309801266608 1101 30-31 1.1753567387213764 1101 32-33 1.3962278616101074 1101 34-35 1.398168496962036 1101 36-37 1.2529935845772684 1101 38-39 1.2240984065798557 1101 40-41 1.1754722137706821 1101 42-43 1.3045671166650834 1101 44-45 1.395441556167789 1101 46-47 1.1543817733579544 1101 48-49 0.935673576434322 1101 50-51 0.8921046241932125 1101 52-53 0.881477810068727 1101 54-55 0.9498762643758987 1101 56-57 1.2019069021840352 1101 58-59 0.9095639081071454 1101 60-61 0.7034410181475863 1101 62-63 0.4243778126675366 1101 64-65 0.7987866416928746 1101 66-67 0.749534989624685 1101 68-69 0.530332235970647 1101 70-71 0.7522269580256378 1101 72-73 0.7425896225344495 1101 74-75 0.6454828398683112 1101 76-77 0.4177809815571045 1101 78-79 0.13667763523535825 1101 80-81 -0.12382100823611353 1101 82-83 -0.34201362350822606 1101 84-85 -0.27126575139239506 1101 86-87 -0.5000144878521162 1101 88-89 -4.464973726210445 1101 90-91 -7.980617321749252 1101 92-93 -10.437232476427756 1101 94-95 -8.404638076922726 1101 96-97 -5.083712856721277 1101 98-99 -3.5251498399929417 1101 100-101 -2.636249133206933 1102 1 1.935252117859239 1102 2 1.2192114945365837 1102 3 1.0978686760686358 1102 4 1.0851606962035305 1102 5 0.996473652366916 1102 6 1.0864341193678442 1102 7 1.0309243600321025 1102 8 1.1692381709448156 1102 9 1.3570539290821841 1102 10-11 1.27040761684826 1102 12-13 1.2546729416995106 1102 14-15 1.6425524231427175 1102 16-17 1.597299756285011 1102 18-19 1.595605014054435 1102 20-21 1.580699928165437 1102 22-23 1.5327952033813261 1102 24-25 1.6663089857188425 1102 26-27 1.7108331810649773 1102 28-29 1.4911829232407925 1102 30-31 1.6054138734996855 1102 32-33 1.8435293986051065 1102 34-35 1.784979667870978 1102 36-37 1.7678097978018315 1102 38-39 1.5688700305110146 1102 40-41 1.4087422389733248 1102 42-43 1.5013393177287497 1102 44-45 1.4535380172555676 1102 46-47 1.6407012313894356 1102 48-49 1.4291001510635333 1102 50-51 1.601528289038253 1102 52-53 1.6663246979754796 1102 54-55 1.5742120817911918 1102 56-57 1.6591968630265796 1102 58-59 1.587294198272577 1102 60-61 1.4317447779067969 1102 62-63 1.3549226288829956 1102 64-65 1.3655030704040563 1102 66-67 1.3617057404419164 1102 68-69 1.2861844690535627 1102 70-71 1.1203833926510889 1102 72-73 1.0932762963125349 1102 74-75 1.1023515096136123 1102 76-77 1.0286752398926922 1102 78-79 0.9113612174235861 1102 80-81 0.7936783574541622 1102 82-83 0.74477770288701 1102 84-85 0.503673564821721 1102 86-87 0.4630906302233022 1102 88-89 0.07190233860350048 1102 90-91 -5.462991887001408 1102 92-93 -10.347325661886519 1102 94-95 -8.268366404301588 1102 96-97 -4.56883097903942 1102 98-99 -2.8349715006872707 1102 100-101 -1.97689729723764 1103 1 2.0414428368259827 1103 2 1.2128511048954351 1103 3 1.0213998929752464 1103 4 0.839450474941259 1103 5 0.8894534546527098 1103 6 0.77550180764689 1103 7 0.7491526255789793 1103 8 0.8828141644892611 1103 9 0.8444369961182474 1103 10-11 0.809771866386015 1103 12-13 0.8179452403819027 1103 14-15 0.9837082118537168 1103 16-17 0.8962842153641049 1103 18-19 0.923944107377757 1103 20-21 0.8375607824237719 1103 22-23 0.8575631890325965 1103 24-25 0.7372821312188051 1103 26-27 0.7387761656325651 1103 28-29 0.6159511528986101 1103 30-31 0.7692362490728542 1103 32-33 0.8273827314332038 1103 34-35 0.7131043440240532 1103 36-37 0.7269810065787112 1103 38-39 0.6690462750090305 1103 40-41 0.4859678729812913 1103 42-43 0.5320171133907792 1103 44-45 0.5753381583774768 1103 46-47 0.804027914826527 1103 48-49 0.6958923232184837 1103 50-51 0.5797952353651361 1103 52-53 0.5358617500835088 1103 54-55 0.3584524807792242 1103 56-57 0.3762284199635033 1103 58-59 0.5134896796731034 1103 60-61 0.3855793480662868 1103 62-63 0.3916143248806847 1103 64-65 0.4509832308053525 1103 66-67 0.49322143307748334 1103 68-69 0.30046276369463243 1103 70-71 0.21177938428367327 1103 72-73 0.36919578477145265 1103 74-75 0.06405737234471331 1103 76-77 0.09024257866823504 1103 78-79 -0.13549406917933382 1103 80-81 -0.2668852173049956 1103 82-83 -0.5542164805708971 1103 84-85 -0.7147360908907743 1103 86-87 -0.5874658410744971 1103 88-89 -1.323812705376529 1103 90-91 -6.709768201601065 1103 92-93 -9.858680793904112 1103 94-95 -8.67372433607908 1103 96-97 -5.422031900063686 1103 98-99 -3.9063371020213253 1103 100-101 -3.1738948396610507 1104 1 1.8241249695081159 1104 2 1.1173061093504408 1104 3 0.8937469653223182 1104 4 0.6739151594059436 1104 5 0.6576331728324263 1104 6 0.7996968818419674 1104 7 0.8143793908057475 1104 8 0.9350944667695629 1104 9 0.9344979361791843 1104 10-11 0.944955676569819 1104 12-13 0.7907710882077552 1104 14-15 0.8054285585740644 1104 16-17 0.8881535572334514 1104 18-19 0.8300150134486657 1104 20-21 0.9027498726128655 1104 22-23 0.9268808583502661 1104 24-25 0.907722076658743 1104 26-27 0.8198610967174957 1104 28-29 0.9234504103978693 1104 30-31 0.9212907761273783 1104 32-33 0.9304069094573819 1104 34-35 0.933948539868247 1104 36-37 0.8744023290000342 1104 38-39 0.7743741303368878 1104 40-41 0.8376173246307488 1104 42-43 0.7969325783062402 1104 44-45 0.6309368889762084 1104 46-47 0.6617248975235128 1104 48-49 0.545750973077137 1104 50-51 0.6452802258501258 1104 52-53 0.6568065960283569 1104 54-55 0.5230959704227125 1104 56-57 0.570540864275948 1104 58-59 0.5661269323103575 1104 60-61 0.6167960042829463 1104 62-63 0.728236661503022 1104 64-65 0.4444794743015983 1104 66-67 0.5674580323140823 1104 68-69 0.48646277469464394 1104 70-71 0.36390706141135 1104 72-73 0.311508152083821 1104 74-75 0.21497449826183868 1104 76-77 0.221054434480088 1104 78-79 0.11939818571292093 1104 80-81 0.12501547459569906 1104 82-83 -0.025606276960694174 1104 84-85 -0.12412597528065916 1104 86-87 -0.04565782426648113 1104 88-89 -0.15557743714126104 1104 90-91 -3.1012376930705585 1104 92-93 -7.905568890792209 1104 94-95 -5.850998038352781 1104 96-97 -3.2797929578247427 1104 98-99 -2.4796378753221013 1104 100-101 -1.8857671515333614 1105 1 1.9071835775667232 1105 2 1.1930226283075172 1105 3 0.9103397771934603 1105 4 0.7825638169114626 1105 5 0.772326635985138 1105 6 0.7538243150449446 1105 7 0.6415203909586751 1105 8 0.8658163018154568 1105 9 0.7067665224636812 1105 10-11 0.6737751589429806 1105 12-13 0.6918375223258764 1105 14-15 0.8053464504899637 1105 16-17 0.6969969559376779 1105 18-19 0.8264623064913863 1105 20-21 0.7242045086549709 1105 22-23 0.579201701302317 1105 24-25 0.6781248128666562 1105 26-27 0.569776773596395 1105 28-29 0.43057318049280724 1105 30-31 0.8561806736166773 1105 32-33 0.9020728539613003 1105 34-35 0.9518398489126341 1105 36-37 0.8827605435709742 1105 38-39 0.7917981272588932 1105 40-41 0.7075201907065676 1105 42-43 0.7829263227989927 1105 44-45 0.7428744819774167 1105 46-47 0.7875887619559663 1105 48-49 0.5861500013737775 1105 50-51 0.5839759817178489 1105 52-53 0.4120943445069578 1105 54-55 0.46919841558082354 1105 56-57 0.4180980729166528 1105 58-59 0.36582362395536094 1105 60-61 0.47497304096395965 1105 62-63 0.3768785403884394 1105 64-65 0.4242604344503498 1105 66-67 0.40219302892780817 1105 68-69 0.3758545073269346 1105 70-71 0.09293427177554037 1105 72-73 0.18730489786068816 1105 74-75 0.16367973327422902 1105 76-77 0.12444365350052067 1105 78-79 -0.29108716227242937 1105 80-81 -0.25500741007430605 1105 82-83 -0.6252336955990465 1105 84-85 -0.6281737581803277 1105 86-87 -0.521889081412251 1105 88-89 -0.551061947660564 1105 90-91 -3.387288664409798 1105 92-93 -7.885674611777652 1105 94-95 -5.553721380837555 1105 96-97 -3.0288678612734046 1105 98-99 -2.0260033637104513 1105 100-101 -1.517878360955704 1106 1 1.5540306480731765 1106 2 1.0200110453721578 1106 3 0.6968770086211791 1106 4 0.7536476910252716 1106 5 0.7826813399362038 1106 6 0.825479044069013 1106 7 0.723053288903678 1106 8 -0.32236370454163676 1106 9 -0.5018847476712196 1106 10-11 -0.7792125997086856 1106 12-13 -0.3803332267644848 1106 14-15 0.5035045722657685 1106 16-17 0.7967149340669195 1106 18-19 0.8349196310643023 1106 20-21 0.8475386103013065 1106 22-23 0.6880174019346725 1106 24-25 0.8087677180966466 1106 26-27 0.8445029201924896 1106 28-29 0.6578653361981068 1106 30-31 0.6987090436151604 1106 32-33 0.7316395204963442 1106 34-35 0.7563506687996693 1106 36-37 0.7324730925077461 1106 38-39 0.6426995459562406 1106 40-41 0.4810456220312389 1106 42-43 0.6717273300622679 1106 44-45 0.499009222689061 1106 46-47 0.5496936401347625 1106 48-49 0.3401388537609442 1106 50-51 0.27665246995821846 1106 52-53 0.39934166495369183 1106 54-55 0.4321910654413408 1106 56-57 0.5064549722357334 1106 58-59 0.41900231743499816 1106 60-61 0.2579641459724371 1106 62-63 0.46999869236634595 1106 64-65 0.36629886918454346 1106 66-67 0.4198285267650128 1106 68-69 0.4002113454442089 1106 70-71 0.24042309751030544 1106 72-73 0.3629029974458966 1106 74-75 0.42219480471253235 1106 76-77 0.3318194335767686 1106 78-79 0.34787758450016426 1106 80-81 0.21908337293370295 1106 82-83 0.0181985362333954 1106 84-85 0.031650439631803806 1106 86-87 0.1361845719999515 1106 88-89 -0.052324111519514105 1106 90-91 -3.3077870247936723 1106 92-93 -8.433265308905707 1106 94-95 -5.77770592442015 1106 96-97 -3.010865892944352 1106 98-99 -1.8952112790782252 1106 100-101 -1.2915717270747802 1107 1 1.8123708619432648 1107 2 1.0705311111708085 1107 3 0.7655545947445361 1107 4 0.7303138617386082 1107 5 0.6388925191484489 1107 6 0.7737392022618153 1107 7 0.559342805902233 1107 8 0.6238480766665973 1107 9 0.6163766291963739 1107 10-11 0.5898927339267885 1107 12-13 0.6421253223860361 1107 14-15 0.6675415646742522 1107 16-17 0.7289447467678585 1107 18-19 0.655149314556347 1107 20-21 0.43489030761184466 1107 22-23 0.45158513113240417 1107 24-25 0.48596162638671103 1107 26-27 0.7503026008899027 1107 28-29 0.42817762283824123 1107 30-31 0.6221334414845856 1107 32-33 0.7689293174475083 1107 34-35 0.6697036860965326 1107 36-37 0.369874454486947 1107 38-39 0.3989106884614202 1107 40-41 0.5298375188716378 1107 42-43 0.3411493111380537 1107 44-45 0.24719711689242274 1107 46-47 0.24919468090261176 1107 48-49 0.3782447101051325 1107 50-51 0.47699165554085354 1107 52-53 0.45980842963639645 1107 54-55 0.46986188180173016 1107 56-57 0.28346611601342175 1107 58-59 0.4032760900361545 1107 60-61 0.2832651684356975 1107 62-63 0.18694452447406817 1107 64-65 0.24899230444281173 1107 66-67 0.4248776457218675 1107 68-69 0.3886513095593749 1107 70-71 0.4328732603184058 1107 72-73 0.28584549263604586 1107 74-75 0.2610279382778202 1107 76-77 0.39597691083184117 1107 78-79 0.24775586384881265 1107 80-81 0.193900975492042 1107 82-83 -0.03212557497826651 1107 84-85 -0.1543416032538012 1107 86-87 0.0752956759871708 1107 88-89 -0.13613192454641165 1107 90-91 -3.302400261951213 1107 92-93 -4.638671232839844 1107 94-95 -2.981033555105892 1107 96-97 -1.705900539526212 1107 98-99 -1.16010651954136 1107 100-101 -0.7893700980638574 1108 1 1.7020546209430414 1108 2 1.007228483446319 1108 3 -0.35874320440114715 1108 4 -0.18720590007384885 1108 5 0.3751289895076084 1108 6 0.4595502758102512 1108 7 0.5328391256781444 1108 8 0.46561906786860874 1108 9 0.7868483993151116 1108 10-11 0.7134023139496364 1108 12-13 0.5614676314717073 1108 14-15 0.5821987598460225 1108 16-17 0.5824616589272438 1108 18-19 0.6293011643174609 1108 20-21 0.4007480318067991 1108 22-23 0.4091913705552628 1108 24-25 0.4031446819289499 1108 26-27 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3.327346709164054 2311 96-97 2.651531791516611 2311 98-99 2.242625412209307 2311 100-101 2.092388158616563 2312 1 1.2234452806429594 2312 2 0.4967741273382984 2312 3 -0.16927183091092246 2312 4 -0.6244279152634746 2312 5 -0.2041868865355596 2312 6 -0.2246161212494613 2312 7 0.014430832899094526 2312 8 0.15722578074215932 2312 9 0.04968560183497317 2312 10-11 -0.05444751349320143 2312 12-13 0.031107187438195183 2312 14-15 0.1153432463532198 2312 16-17 -0.20197900550277126 2312 18-19 -0.628311447329871 2312 20-21 0.11385396146996385 2312 22-23 0.304739745883694 2312 24-25 0.32675864518260767 2312 26-27 0.32923822823016025 2312 28-29 0.237369606572976 2312 30-31 0.24206870033820138 2312 32-33 0.4436730632273225 2312 34-35 0.31096530128090905 2312 36-37 0.3459611659623505 2312 38-39 0.4354161892835364 2312 40-41 0.3774556603674242 2312 42-43 0.5515281536329866 2312 44-45 0.3054223999976031 2312 46-47 0.3893165583519078 2312 48-49 0.5479198969357633 2312 50-51 0.3417007543482278 2312 52-53 0.2614634663784905 2312 54-55 0.3633518853108164 2312 56-57 0.5137587278014308 2312 58-59 0.37045721341281634 2312 60-61 0.4104576034951464 2312 62-63 0.5117784033595214 2312 64-65 0.5054149474082656 2312 66-67 0.6104817495687413 2312 68-69 0.6730299920911094 2312 70-71 0.7123204402750183 2312 72-73 0.5415082555123689 2312 74-75 0.5752938094059381 2312 76-77 0.9742014929994198 2312 78-79 1.0431471221680546 2312 80-81 1.0723223585276358 2312 82-83 1.042744669165554 2312 84-85 1.381166599583917 2312 86-87 1.8234047374844486 2312 88-89 2.2054691399998347 2312 90-91 2.946720654620293 2312 92-93 3.7813872723788435 2312 94-95 3.573569045130931 2312 96-97 2.772375503327222 2312 98-99 2.4194212242942896 2312 100-101 2.292095002164727 2313 1 -7.3978273753357975 2313 2 -2.6344059001928954 2313 3 -1.5128237063085948 2313 4 -0.8147263195969785 2313 5 -0.5192159485950043 2313 6 -0.10448568258156143 2313 7 -0.08037164129468266 2313 8 -0.04700322135222024 2313 9 0.012352611624223186 2313 10-11 -0.018358920479712992 2313 12-13 -0.16934777552556568 2313 14-15 -0.22316533748971779 2313 16-17 -0.3692006013255309 2313 18-19 -0.48633824507327006 2313 20-21 -0.3646913875994855 2313 22-23 -1.0154030959818883 2313 24-25 -0.3866218658779701 2313 26-27 -0.4216288724833177 2313 28-29 -0.5942490253738484 2313 30-31 -0.09516037646835684 2313 32-33 -0.17599164513394783 2313 34-35 -0.021783144779099928 2313 36-37 0.04299611807574877 2313 38-39 -0.059399860063614085 2313 40-41 -0.16803311595945303 2313 42-43 -0.08783285706159916 2313 44-45 -0.15589288098609444 2313 46-47 -0.9681713106859497 2313 48-49 -0.5177492482544075 2313 50-51 -0.5083797687737253 2313 52-53 -0.9371650280637525 2313 54-55 -0.592082692948722 2313 56-57 -0.2968657585523644 2313 58-59 -0.2183087123662517 2313 60-61 0.010049738500534033 2313 62-63 -0.11377902027169284 2313 64-65 -0.054070837501729585 2313 66-67 -0.00915090092135884 2313 68-69 -0.045659453813126305 2313 70-71 0.04307006708037875 2313 72-73 0.024914290791162585 2313 74-75 0.19580464535704678 2313 76-77 0.38682133157228904 2313 78-79 0.12486300202111167 2313 80-81 0.2717988117404815 2313 82-83 0.6235100685411972 2313 84-85 0.8485009961414924 2313 86-87 1.3285153920754134 2313 88-89 1.7732840628045778 2313 90-91 2.396108869336249 2313 92-93 3.3520213101714873 2313 94-95 3.004385400163187 2313 96-97 2.0508393282292303 2313 98-99 1.5521351709087767 2313 100-101 1.186976436872877 2314 1 0.5646195881580738 2314 2 0.020226220522978622 2314 3 -1.3379303110151533 2314 4 -0.2640420290366805 2314 5 -0.09726494954142595 2314 6 -3.320446081990319 2314 7 -2.838037609669506 2314 8 -2.7634006734731464 2314 9 -3.2960598555436533 2314 10-11 -2.418701863301308 2314 12-13 -1.0988464409728778 2314 14-15 -1.2423198903879822 2314 16-17 -2.166676914344592 2314 18-19 -3.1979430048006208 2314 20-21 -2.923677287066731 2314 22-23 -3.9607752288203883 2314 24-25 -2.9655949515126494 2314 26-27 -1.822255740059532 2314 28-29 -1.7755905432547294 2314 30-31 -1.8787171986378794 2314 32-33 -2.1440865312982 2314 34-35 -1.6562058311113717 2314 36-37 -1.90462853765219 2314 38-39 -3.2322031650753615 2314 40-41 -2.6512451343773478 2314 42-43 -1.5620674831210053 2314 44-45 -2.020306581684739 2314 46-47 -1.522247279167111 2314 48-49 -2.085954726832444 2314 50-51 -3.04437494098951 2314 52-53 -1.8539492877857846 2314 54-55 -2.4767044987952325 2314 56-57 -1.340506960606831 2314 58-59 -0.895014919365476 2314 60-61 -1.3489903937850087 2314 62-63 -1.0576597506069803 2314 64-65 -1.5572237846832166 2314 66-67 -1.017700013523573 2314 68-69 -0.9284511678892002 2314 70-71 -0.8208358655645895 2314 72-73 -2.024464424422735 2314 74-75 -2.054008729944691 2314 76-77 -1.2298536649716993 2314 78-79 -1.9496816018134702 2314 80-81 -0.8801211363661565 2314 82-83 -0.5483098678681664 2314 84-85 -1.650894999137062 2314 86-87 -0.40758505561118596 2314 88-89 0.7681861778844876 2314 90-91 1.9230689603623112 2314 92-93 2.9858375991579713 2314 94-95 2.7501980417267973 2314 96-97 1.8523137165149386 2314 98-99 1.7813610771817068 2314 100-101 1.79501140108985 2315 1 -2.856932998025261 2315 2 -1.987519347524195 2315 3 -2.768890397315044 2315 4 -4.328916662688172 2315 5 -4.090331658329124 2315 6 -3.248561189776762 2315 7 -5.683357753652711 2315 8 -3.142606748226733 2315 9 -2.5874395525615768 2315 10-11 -3.973968841535026 2315 12-13 -3.0767752998304374 2315 14-15 -2.1262008775635763 2315 16-17 -2.462591053347225 2315 18-19 -2.09064117462556 2315 20-21 -1.4862558620895996 2315 22-23 -0.9283149021733692 2315 24-25 -2.435348491206902 2315 26-27 -4.0035573564140705 2315 28-29 -3.42894004977947 2315 30-31 -3.2359656741618608 2315 32-33 -4.036224728485735 2315 34-35 -3.893127912822962 2315 36-37 -3.1488702545184495 2315 38-39 -1.961356668918505 2315 40-41 -1.6406218314772651 2315 42-43 -3.070370109898086 2315 44-45 -6.255898816302121 2315 46-47 -3.0931541032571275 2315 48-49 -2.232458158410683 2315 50-51 -3.218896899392572 2315 52-53 -3.1935151678180027 2315 54-55 -1.8814472550221524 2315 56-57 -1.5050698865397223 2315 58-59 -2.7483049017936096 2315 60-61 -3.0073330315264215 2315 62-63 -4.8595528258766265 2315 64-65 -4.325549449415853 2315 66-67 -3.871419847547404 2315 68-69 -2.292961603295307 2315 70-71 -3.5610231313467153 2315 72-73 -2.747134685042621 2315 74-75 -1.7401269349133628 2315 76-77 -3.406638523745656 2315 78-79 -1.8557501859518446 2315 80-81 -1.7303794492418518 2315 82-83 -1.424176604629384 2315 84-85 -2.91323479451243 2315 86-87 -2.2927674541629948 2315 88-89 -1.5155946512568335 2315 90-91 -0.7773388460159723 2315 92-93 -0.1743745614421428 2315 94-95 -0.1627369142310009 2315 96-97 -0.4615642602927643 2315 98-99 -0.7580371627377804 2315 100-101 -1.1499805565254544 2316 1 -7.683533252353133 2316 2 -6.227548778514244 2316 3 -3.5431062872922894 2316 4 -4.2019589219890605 2316 5 -2.169587108617595 2316 6 -2.961858214886771 2316 7 -3.40088877824693 2316 8 -2.545742174574645 2316 9 -7.118775771413308 2316 10-11 -6.837747313524087 2316 12-13 -6.112446081483146 2316 14-15 -4.803921804627592 2316 16-17 -4.974376628026036 2316 18-19 -4.184059934063509 2316 20-21 -2.8758840738305267 2316 22-23 -3.5113530335123038 2316 24-25 -3.2056984718241353 2316 26-27 -4.037560834769437 2316 28-29 -4.249338223744278 2316 30-31 -6.9063081733549225 2316 32-33 -4.545865628235461 2316 34-35 -5.406988923776254 2316 36-37 -4.202918171316902 2316 38-39 -6.296043146558574 2316 40-41 -3.6183989604594515 2316 42-43 -4.381569781607517 2316 44-45 -2.9596731441093382 2316 46-47 -4.042834530878302 2316 48-49 -5.218981254722767 2316 50-51 -4.580359576615145 2316 52-53 -6.256991098312529 2316 54-55 -5.1585121686431314 2316 56-57 -4.375538150703335 2316 58-59 -2.9368607152454125 2316 60-61 -3.0931667622470513 2316 62-63 -4.204868400052618 2316 64-65 -3.0409877027283585 2316 66-67 -4.073500007864791 2316 68-69 -3.139329544196517 2316 70-71 -2.468409486688813 2316 72-73 -1.8901283211688558 2316 74-75 -1.952426943672105 2316 76-77 -2.755848392088808 2316 78-79 -4.6792772780337835 2316 80-81 -5.621746534543874 2316 82-83 -3.2652229010608416 2316 84-85 -1.9378779772116452 2316 86-87 -2.0663416447499436 2316 88-89 -1.113579208299754 2316 90-91 -0.9898677579695452 2316 92-93 -1.027894865500194 2316 94-95 -1.5506135806219294 2316 96-97 -3.1248394493761324 2316 98-99 -4.596646623475104 2316 100-101 -3.861180856755615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32259.0 3 10712.0 4 9761.0 5 10858.0 6 12526.0 7 14656.0 8 17720.0 9 21233.0 10 25436.0 11 30471.0 12 35796.0 13 40956.0 14 45491.0 15 50763.0 16 57451.0 17 61625.0 18 56003.0 19 53648.0 20 57370.0 21 62417.0 22 69616.0 23 77608.0 24 87718.0 25 98413.0 26 111106.0 27 126124.0 28 142702.0 29 162598.0 30 184186.0 31 209552.0 32 234777.0 33 264382.0 34 299925.0 35 347833.0 36 377945.0 37 304068.0 38 90230.0 39 1418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.824072156551594 20.181491075755233 13.852530664324759 22.14190610336841 2 15.343475346696907 20.485207401578563 41.21634796575279 22.95496928597174 3 18.883789927590296 26.08963963686215 29.947491146613718 25.07907928893383 4 12.18518822093847 16.930236191217173 38.44148689749374 32.44308869035063 5 12.766064585910513 38.53727903687632 34.668033455496506 14.028622921716655 6 30.47793040716587 37.419612619418366 18.099943984500666 14.002512988915097 7 27.562046985040865 33.73785534364879 21.46062304014805 17.239474631162288 8 26.10457857656481 35.26407036325255 20.148173200429078 18.483177859753564 9 27.068183021506997 16.643800372885938 20.513275901153868 35.774740704453194 10-11 24.49233976682749 27.181652038235498 27.90566750082692 20.420340694110084 12-13 25.74821115973179 24.844269903385666 28.12863305980344 21.278885877079098 14-15 22.75260065040032 25.961954099850377 26.709331090975347 24.576114158773954 16-17 21.40198406748096 29.242695882238284 27.36581486705319 21.989505183227568 18-19 21.418635816186324 27.720569677025036 30.092647566057686 20.76814694073095 20-21 22.618883301859707 26.758262758642136 30.008476930950025 20.61437700854813 22-23 22.10752325437437 26.697704639330126 29.919614873388955 21.27515723290654 24-25 21.56754755635441 26.947449959950987 30.348433485012517 21.136568998682094 26-27 21.302547080576637 27.167140467851077 30.20846180407973 21.321850647492564 28-29 21.26274852125641 27.456599602170524 30.18841088882019 21.09224098775288 30-31 21.56021485300222 27.177796362847072 30.05152984780988 21.210458936340824 32-33 21.282746049282895 27.322120003867905 29.965849777081004 21.42928416976819 34-35 21.62383381849227 27.29172023987081 30.02003209249221 21.064413849144714 36-37 21.665778779720586 26.964927244061926 29.94350827342186 21.42578570279563 38-39 21.174580444076334 27.331174455730263 30.053574227285402 21.440670872908004 40-41 21.50940286131498 27.241663198316473 30.040142858740882 21.20879108162767 42-43 21.361242435727792 27.22501585237976 29.718095060794454 21.695646651097995 44-45 21.419667889164746 27.406433130452136 29.706574749621012 21.46732423076211 46-47 21.64155423223788 27.457208828323832 29.295131471585318 21.606105467852974 48-49 21.3702696431392 27.63426719824577 29.191433303338115 21.804029855276923 50-51 21.422004105256956 27.781910989652992 29.02674951269689 21.769335392393156 52-53 21.471060265018984 27.81176364053355 28.835558546603036 21.881617547844435 54-55 21.465762042656007 27.824687272772643 28.86965174410924 21.839898940462106 56-57 21.400055475905212 27.916442536208187 28.731799460132258 21.95170252775435 58-59 21.34615362358463 27.678828504849147 28.842682508266442 22.13233536329978 60-61 21.238671720227263 27.873501229407772 28.858145909606197 22.02968114075877 62-63 21.216162712073572 27.739068843085484 29.023066817154596 22.02170162768635 64-65 21.1112115236065 27.626181334082702 28.924133531935826 22.33847361037498 66-67 21.165047744865674 27.770456961226614 28.591959712168062 22.472535581739653 68-69 21.36020322762703 27.59889798379373 28.688816695314117 22.35208209326513 70-71 21.40780862508291 27.454667266821797 28.55158319200503 22.585940916090262 72-73 21.386592691049554 27.499985531542205 28.484560869444998 22.628860907963247 74-75 21.247011403929577 27.696797202455443 28.392222126478543 22.663969267136437 76-77 21.368802717276797 27.511552876970573 28.393065109526912 22.726579296225722 78-79 21.394375226382166 27.674203649280514 28.34480936563601 22.58661175870131 80-81 21.585339371731465 27.625427035193518 28.06705050734791 22.722183085727107 82-83 21.64931889114029 27.45855849678655 28.164257540464455 22.7278650716087 84-85 21.551690987022898 27.439609811105946 28.116611669938347 22.892087531932805 86-87 21.57547887753085 27.406188131896286 28.154923615861847 22.863409374711015 88-89 21.662380391044675 27.283684563555532 28.055201313471766 22.998733731928027 90-91 21.558468601317106 27.232327626418634 28.24846752408692 22.96073624817734 92-93 21.945614909644014 26.92112686925117 28.208508827837587 22.92474939326723 94-95 21.673217069581884 26.781707447376856 28.306700463570277 23.23837501947098 96-97 21.97001307085603 26.714518943220195 27.925300424471516 23.39016756145226 98-99 22.03470300312173 26.794802297231623 27.576818058266824 23.593676641379822 100-101 22.303624713604155 26.72142262159989 27.71397835704073 23.260974307755227 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5647.0 1 3362.0 2 1114.5 3 1135.0 4 1031.0 5 974.5 6 1277.5 7 2284.0 8 3651.5 9 4280.5 10 4138.0 11 3927.0 12 3952.5 13 4236.0 14 4640.5 15 5202.5 16 5670.0 17 6143.5 18 6828.5 19 7585.0 20 8527.0 21 9970.0 22 11685.0 23 14078.0 24 17073.5 25 20601.5 26 25874.5 27 32697.5 28 38668.5 29 44723.5 30 52343.0 31 60692.5 32 71540.5 33 81525.0 34 89702.0 35 97996.0 36 107559.0 37 115669.0 38 119110.0 39 121678.5 40 125394.0 41 129078.0 42 130712.5 43 134080.5 44 138492.5 45 141543.5 46 142231.0 47 141516.5 48 142612.0 49 141080.5 50 136741.5 51 132497.5 52 127609.5 53 122645.0 54 116974.5 55 107986.0 56 97031.5 57 86733.5 58 77463.5 59 67622.0 60 56559.0 61 45966.5 62 36164.0 63 27148.5 64 19932.0 65 14623.5 66 10834.0 67 8121.0 68 5883.5 69 4193.5 70 3071.0 71 2271.5 72 1707.5 73 1277.5 74 983.5 75 783.5 76 550.5 77 383.5 78 255.5 79 181.5 80 123.0 81 80.5 82 65.0 83 46.5 84 34.0 85 21.0 86 13.5 87 11.0 88 7.0 89 5.5 90 6.5 91 5.5 92 4.5 93 4.0 94 3.0 95 1.5 96 2.0 97 4.0 98 3.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13781148384557415 2 0.12398158442409501 3 0.1297547335332468 4 0.14019771881069024 5 0.12875405435432716 6 0.14299448882356822 7 0.17327144859600863 8 0.1598777426627765 9 0.16983321757100267 10-11 0.1645732372715533 12-13 0.1540789351131396 14-15 0.15936457385307412 16-17 0.15404044745241194 18-19 0.16901214747547885 20-21 0.1743234446558985 22-23 0.208821217888141 24-25 0.2307335260624326 26-27 0.24299826061431953 28-29 0.23746886668977638 30-31 0.22905289821065733 32-33 0.22951475013938946 34-35 0.2204829790886276 36-37 0.22855255862119753 38-39 0.216800992879013 40-41 0.22108595244002788 42-43 0.2141710027292883 44-45 0.20652478746472283 46-47 0.22174024267239842 48-49 0.258303520363693 50-51 0.2602279034000769 52-53 0.26165194684700105 54-55 0.26016375729886415 56-57 0.23550599599266478 58-59 0.23589087259994154 60-61 0.20737151600073175 62-63 0.19969964229568118 64-65 0.20120066106406065 66-67 0.20883404710838357 68-69 0.20829521985819607 70-71 0.21446607479486718 72-73 0.24594898127010817 74-75 0.24780921820527932 76-77 0.26238321240082696 78-79 0.25968907614988945 80-81 0.2738012184167049 82-83 0.2729929775414236 84-85 0.24882272660444152 86-87 0.2514142290934386 88-89 0.25518601984475103 90-91 0.43698889990206174 92-93 0.5826133788753547 94-95 0.3430020324050708 96-97 0.2880544821061885 98-99 0.3163429127410322 100-101 0.33580483984899495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3897353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.92245078600568 #Duplication Level Percentage of deduplicated Percentage of total 1 71.96069807457816 35.20493710080164 2 13.360416440018138 13.072486315346572 3 5.540264187952167 8.131299062296788 4 2.921123392406965 5.716340616195188 5 1.6988002322191678 4.155473537799863 6 1.0986027753997327 3.2247804127717608 7 0.7254955646468126 2.484511473982937 8 0.4957438510749733 1.940240332534425 9 0.3783241314380715 1.6657689331293655 >10 1.6704600422544191 13.785887209258906 >50 0.09054504044460678 3.02894898014112 >100 0.0551758133180974 4.939479198949749 >500 0.003168849413624774 1.0408414114872409 >1k 0.0010204769029320058 0.6604450251273817 >5k 5.370931068063161E-5 0.20907742450521402 >10k+ 1.0741862136126322E-4 0.7394829656717655 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16553 0.4247241653501748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11590 0.29738132522252925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7957 0.2041642109400919 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.5658440485119002E-5 0.0 0.0 0.0 0.0 3 2.5658440485119002E-5 0.0 0.0 0.0 0.0 4 2.5658440485119002E-5 2.5658440485119002E-5 0.0 0.0 0.0 5 2.5658440485119002E-5 2.5658440485119002E-5 0.0 0.0 0.0 6 7.6975321455357E-5 2.5658440485119002E-5 0.0 0.0 0.0 7 7.6975321455357E-5 2.5658440485119002E-5 0.0 0.0 0.0 8 7.6975321455357E-5 5.1316880970238005E-5 0.0 0.0 0.0 9 7.6975321455357E-5 5.1316880970238005E-5 0.0 0.0 0.0 10-11 7.6975321455357E-5 5.1316880970238005E-5 0.0 2.5658440485119002E-5 0.0 12-13 7.6975321455357E-5 5.1316880970238005E-5 0.0 5.1316880970238005E-5 0.0 14-15 1.0263376194047601E-4 5.1316880970238005E-5 2.5658440485119002E-5 1.1546298218303552E-4 0.0 16-17 1.2829220242559501E-4 5.1316880970238005E-5 2.5658440485119002E-5 1.53950642910714E-4 0.0 18-19 2.30925964366071E-4 5.1316880970238005E-5 2.5658440485119002E-5 1.667798631532735E-4 0.0 20-21 3.07901285821428E-4 5.1316880970238005E-5 2.5658440485119002E-5 3.5921816679166605E-4 0.0 22-23 3.07901285821428E-4 5.1316880970238005E-5 2.5658440485119002E-5 8.852161967366056E-4 0.0 24-25 4.1053504776190404E-4 5.1316880970238005E-5 2.5658440485119002E-5 0.001975699917354163 0.0 26-27 4.1053504776190404E-4 5.1316880970238005E-5 2.5658440485119002E-5 0.003681986209614577 0.0 28-29 6.799486728556536E-4 5.1316880970238005E-5 2.5658440485119002E-5 0.010186400872592243 0.0 30-31 7.184363335833321E-4 5.1316880970238005E-5 2.5658440485119002E-5 0.033830653779629405 0.0 32-33 7.184363335833321E-4 5.1316880970238005E-5 2.5658440485119002E-5 0.08616104314902961 0.0 34-35 8.723869764940462E-4 5.1316880970238005E-5 2.5658440485119002E-5 0.15990340110326162 0.0 36-37 9.750207384345221E-4 5.1316880970238005E-5 2.5658440485119002E-5 0.27804769031699206 0.0 38-39 0.0010263376194047601 5.1316880970238005E-5 2.5658440485119002E-5 0.45837521004640847 0.0 40-41 0.0010263376194047601 5.1316880970238005E-5 2.5658440485119002E-5 0.6612308405217593 0.0 42-43 0.0011033129408601172 5.1316880970238005E-5 2.5658440485119002E-5 0.8662161215573749 0.0 44-45 0.001180288262315474 5.1316880970238005E-5 2.5658440485119002E-5 1.105994247890812 0.0 46-47 0.0013470681254687477 5.1316880970238005E-5 2.5658440485119002E-5 1.3874929984530526 0.0 48-49 0.001411214226681545 7.6975321455357E-5 2.5658440485119002E-5 1.683706864633509 0.0 50-51 0.001411214226681545 7.6975321455357E-5 2.5658440485119002E-5 2.001217236416614 0.0 52-53 0.001411214226681545 7.6975321455357E-5 2.5658440485119002E-5 2.30865667030931 0.0 54-55 0.0014368726671666642 7.6975321455357E-5 2.5658440485119002E-5 2.6320941418444774 0.0 56-57 0.001462531107651783 7.6975321455357E-5 2.5658440485119002E-5 2.99966413101405 0.0 58-59 0.001462531107651783 7.6975321455357E-5 2.5658440485119002E-5 3.402706914154299 0.0 60-61 0.001462531107651783 7.6975321455357E-5 2.5658440485119002E-5 3.8301508741958967 0.0 62-63 0.0015010187683794616 7.6975321455357E-5 2.5658440485119002E-5 4.2481396989187274 0.0 64-65 0.001565164869592259 7.6975321455357E-5 2.5658440485119002E-5 4.666423595707137 0.0 66-67 0.0016421401910476161 7.6975321455357E-5 2.5658440485119002E-5 5.125196511581065 0.0 68-69 0.0016677986315327352 7.6975321455357E-5 2.5658440485119002E-5 5.632502367632595 0.0 70-71 0.0017062862922604138 7.6975321455357E-5 2.5658440485119002E-5 6.174639556642675 0.0 72-73 0.00179609083395833 7.6975321455357E-5 2.5658440485119002E-5 6.707116342810107 0.0 74-75 0.001847407714928568 7.6975321455357E-5 2.5658440485119002E-5 7.22510893932369 0.0 76-77 0.001847407714928568 7.6975321455357E-5 2.5658440485119002E-5 7.7688754393045745 0.0 78-79 0.001847407714928568 7.6975321455357E-5 2.5658440485119002E-5 8.364138429339093 0.0 80-81 0.002103992119779758 7.6975321455357E-5 2.5658440485119002E-5 8.975155701831474 0.0 82-83 0.002103992119779758 1.0263376194047601E-4 2.5658440485119002E-5 9.564773834959265 0.0 84-85 0.002168138220992556 1.0263376194047601E-4 2.5658440485119002E-5 10.119445172146325 0.0 86-87 0.0022322843222053533 1.0263376194047601E-4 2.5658440485119002E-5 10.677157034530872 0.0 88-89 0.0022322843222053533 1.0263376194047601E-4 2.5658440485119002E-5 11.267942626700737 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9375 0.0 46.75715 1 GTATCAA 17055 0.0 40.66708 1 TATCAAC 21470 0.0 32.87897 2 ATCAACG 21505 0.0 32.80635 3 TCAACGC 21725 0.0 32.605587 4 CAACGCA 22100 0.0 32.074615 5 AACGCAG 22610 0.0 31.26679 6 ACGCAGA 25460 0.0 27.543148 7 CGCAGAG 25650 0.0 27.265137 8 TACATGG 18865 0.0 27.108736 2 GTACATG 18955 0.0 26.404385 1 ACATGGG 19525 0.0 25.247066 3 GCAGAGT 26750 0.0 24.547962 9 GAGTACT 14165 0.0 23.494682 12-13 CATGGGG 8825 0.0 21.934988 4 AGTACTT 14945 0.0 21.855791 12-13 GTACTTT 15335 0.0 21.689032 14-15 AGAGTAC 23560 0.0 21.052944 10-11 CAGAGTA 26265 0.0 20.392977 10-11 CATGGGA 10345 0.0 20.225536 4 >>END_MODULE