FastQCFastQC Report
Fri 27 May 2016
SRR939887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897353
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180250.46249338974427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138900.3563957383383029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99720.2558659685176067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93850.045.788711
GTATCAA168200.039.8512951
ATCAACG213200.031.1438793
TATCAAC214250.031.0588762
TCAACGC214200.030.9748744
CAACGCA219000.030.274445
AACGCAG222550.029.8132296
ACGCAGA256550.025.6784677
CGCAGAG256650.025.5766588
GTACATG198200.025.4418351
TACATGG194800.025.3476262
ACATGGG195350.024.2369253
GCAGAGT275850.023.1982469
GAGTACT147550.021.20606212-13
AGAGTAC246900.020.78428810-11
CATGGGG85100.020.5579264
CATGGGA103650.020.2453674
AGTACTT157350.020.2446912-13
GTACTTT158700.019.76082614-15
CAGAGTA271150.019.26435510-11
Produced by FastQC (version 0.11.5)