##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939887_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3897353 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.394949341258027 31.0 31.0 34.0 30.0 34.0 2 31.603923226867057 31.0 31.0 34.0 30.0 34.0 3 31.657672527995285 31.0 31.0 34.0 30.0 34.0 4 35.476723560837314 37.0 35.0 37.0 33.0 37.0 5 35.20580481162471 37.0 35.0 37.0 32.0 37.0 6 35.23541336902252 37.0 35.0 37.0 32.0 37.0 7 35.15531284951607 37.0 35.0 37.0 32.0 37.0 8 35.093949662758284 37.0 35.0 37.0 32.0 37.0 9 36.68741373953039 39.0 35.0 39.0 32.0 39.0 10-11 36.54010234638741 38.0 35.0 39.0 32.0 39.0 12-13 36.483373972026655 38.0 35.0 39.0 32.0 39.0 14-15 37.498192747744426 39.0 36.0 41.0 32.0 41.0 16-17 37.43577756492676 39.0 36.0 41.0 32.0 41.0 18-19 37.44035964922859 39.0 36.0 41.0 32.0 41.0 20-21 37.38152574324163 39.0 36.0 41.0 32.0 41.0 22-23 37.23302110432388 39.0 36.0 40.0 31.0 41.0 24-25 37.071519054086195 39.0 36.0 40.0 31.0 41.0 26-27 36.77867491089465 39.0 35.5 40.0 30.5 41.0 28-29 36.55804311285121 38.0 35.0 40.0 30.0 41.0 30-31 36.291401753959676 38.0 35.0 40.0 30.0 41.0 32-33 36.22037200633353 38.0 35.0 40.0 30.0 41.0 34-35 36.270258685831124 38.0 35.0 40.0 30.0 41.0 36-37 36.229993536638844 38.0 35.0 40.0 30.0 41.0 38-39 36.09401534836593 38.0 35.0 40.0 30.0 41.0 40-41 35.91540566122699 38.0 35.0 40.0 29.0 41.0 42-43 35.69333673393198 38.0 34.5 40.0 28.5 41.0 44-45 35.41557154304473 38.0 34.0 40.0 27.0 41.0 46-47 35.081876340172414 38.0 34.0 40.0 26.5 41.0 48-49 34.749001950811234 38.0 33.5 40.0 25.5 41.0 50-51 34.47564834388879 38.0 33.0 40.0 24.0 41.0 52-53 34.194829285415 38.0 33.0 40.0 24.0 41.0 54-55 33.89387373943289 37.5 33.0 40.0 23.0 41.0 56-57 32.67364888425554 36.5 31.5 39.5 15.5 41.0 58-59 29.44943850351764 34.5 24.0 39.0 7.0 40.0 60-61 29.402962343929328 34.0 24.5 38.0 7.5 40.0 62-63 31.20646436183738 35.0 29.5 38.5 8.0 40.0 64-65 30.778324031721016 35.0 29.0 38.0 2.0 40.0 66-67 25.411222565674706 28.5 18.0 36.0 2.0 39.0 68-69 17.708079304081515 13.5 7.0 29.5 2.0 35.5 70-71 16.544018722450854 15.0 7.5 26.0 2.0 33.5 72-73 18.731459275051556 19.5 7.5 30.0 2.0 35.0 74-75 19.4470879337848 19.5 8.5 31.0 2.0 35.5 76-77 20.435968848600574 23.5 9.0 31.5 2.0 34.5 78-79 23.53591360597821 29.0 12.0 34.0 2.0 36.0 80-81 26.16237379575317 31.5 22.5 35.0 2.0 36.0 82-83 27.044394875188367 33.0 26.0 35.0 2.0 36.0 84-85 26.99802917518634 33.0 26.0 35.0 2.0 36.0 86-87 26.701023746116917 33.0 25.5 35.0 2.0 36.0 88-89 26.258077084626414 33.0 24.5 35.0 2.0 35.0 90-91 24.697306992720442 31.5 11.5 34.5 2.0 35.0 92-93 22.685024040675813 31.0 2.0 34.0 2.0 35.0 94-95 18.291654489598454 24.0 2.0 33.0 2.0 35.0 96-97 16.985775730348266 15.5 2.0 32.5 2.0 34.5 98-99 15.889197360362276 4.5 2.0 32.0 2.0 34.0 100-101 15.238598351239931 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40279645110659246 1101 2 0.391860513353226 1101 3 0.38312424257428646 1101 4 0.3748048618382853 1101 5 0.4210129447312525 1101 6 0.4897883626433668 1101 7 0.4441578797575332 1101 8 0.4351017076376209 1101 9 0.5579391581897468 1101 10-11 0.5472886654234799 1101 12-13 0.5457297610885448 1101 14-15 0.6015846406319127 1101 16-17 0.5498975828788701 1101 18-19 0.6437630050006149 1101 20-21 0.7187276454597935 1101 22-23 0.5856523689801492 1101 24-25 0.5244368172697804 1101 26-27 0.5967573666065107 1101 28-29 0.5846079159430033 1101 30-31 0.6590914356658786 1101 32-33 0.5924378433485273 1101 34-35 0.8733433781513043 1101 36-37 0.7362563810859157 1101 38-39 0.712990587888612 1101 40-41 0.639040822861098 1101 42-43 0.5866519180606602 1101 44-45 0.5706367431889205 1101 46-47 0.4242543926430855 1101 48-49 0.6001039212123018 1101 50-51 0.6210946899684586 1101 52-53 0.7130972281367463 1101 54-55 0.6817233847880573 1101 56-57 1.4182978935999486 1101 58-59 -7.944305956911673 1101 60-61 -9.153802216168682 1101 62-63 -2.3204113966814397 1101 64-65 0.367507900222833 1101 66-67 -0.8713325229070357 1101 68-69 -3.5096932795862745 1101 70-71 -4.081912066857624 1101 72-73 -6.529431504445185 1101 74-75 -7.310827941406416 1101 76-77 -8.35486152769047 1101 78-79 -11.571570506461635 1101 80-81 -7.54605140824421 1101 82-83 -3.789799500939182 1101 84-85 -2.8803000153964824 1101 86-87 -2.600436752174076 1101 88-89 -2.6590266679613848 1101 90-91 -8.403405981715359 1101 92-93 -14.053624455565547 1101 94-95 -12.438590091687576 1101 96-97 -12.54251481039694 1101 98-99 -11.94322812248341 1101 100-101 -11.63131654184887 1102 1 0.4957425919572991 1102 2 0.5251773412328298 1102 3 0.5103139512117103 1102 4 0.45399876585382515 1102 5 0.49620544879842754 1102 6 0.47764792021680336 1102 7 0.41881374439762453 1102 8 0.5475462837087761 1102 9 0.6297516178195082 1102 10-11 0.68009759882721 1102 12-13 0.655907405780404 1102 14-15 0.7443300693582842 1102 16-17 0.794619522937495 1102 18-19 0.8281500295251831 1102 20-21 0.8998320054904099 1102 22-23 0.7763474250272253 1102 24-25 0.7597093850716803 1102 26-27 0.8812716327682253 1102 28-29 0.7229607498333692 1102 30-31 0.7598085406763033 1102 32-33 0.6299322484687409 1102 34-35 0.7498905690079525 1102 36-37 0.7183181467719066 1102 38-39 0.6154013110285064 1102 40-41 0.6084967995327872 1102 42-43 0.7031973399972316 1102 44-45 0.6092352157532375 1102 46-47 0.59822226574898 1102 48-49 0.6142054750924473 1102 50-51 0.5390701788113077 1102 52-53 0.5208122658334986 1102 54-55 0.5236229145948101 1102 56-57 1.0537048166885512 1102 58-59 -8.608861086913898 1102 60-61 -9.663939613461878 1102 62-63 -2.7267086628065265 1102 64-65 0.12937887269110604 1102 66-67 -1.0027990201862025 1102 68-69 -3.5224000221199567 1102 70-71 -3.988919413682993 1102 72-73 -6.650950562325498 1102 74-75 -7.733132984972773 1102 76-77 -8.66314822585821 1102 78-79 -11.631873762346643 1102 80-81 -7.533345614941627 1102 82-83 -3.3338636461800277 1102 84-85 -2.4497205243272937 1102 86-87 -2.3006345049308656 1102 88-89 -2.3514027564502733 1102 90-91 -8.10723778198463 1102 92-93 -14.106317390695317 1102 94-95 -12.330806351993992 1102 96-97 -12.260232242389556 1102 98-99 -11.799965139458788 1102 100-101 -11.517466712889146 1103 1 0.27569855882178373 1103 2 0.25207236071460315 1103 3 0.23954023800897417 1103 4 0.1652460039149446 1103 5 0.17788196328251615 1103 6 0.14733258911282832 1103 7 0.229568290167947 1103 8 0.3534429460723132 1103 9 0.30551032748989115 1103 10-11 0.3270509042978631 1103 12-13 0.35016328339590075 1103 14-15 0.4785498067326017 1103 16-17 0.4006361719352185 1103 18-19 0.45196957605765675 1103 20-21 0.481538353363959 1103 22-23 0.3864683718989568 1103 24-25 0.4626992619574608 1103 26-27 0.35685508114347186 1103 28-29 0.39701787262865906 1103 30-31 0.549465454544567 1103 32-33 0.3245051910700809 1103 34-35 0.3276335327919284 1103 36-37 0.10489371000362269 1103 38-39 0.1933476973345094 1103 40-41 0.24185391632838815 1103 42-43 0.25919537273028936 1103 44-45 0.20867606756002033 1103 46-47 0.2317201527957593 1103 48-49 0.3120272931824033 1103 50-51 0.3096173247527787 1103 52-53 0.2820961335842469 1103 54-55 0.08852036868784552 1103 56-57 0.7091705419492271 1103 58-59 -6.795795296718772 1103 60-61 -8.743231014125517 1103 62-63 -2.6414754513903524 1103 64-65 -0.5175746121961282 1103 66-67 -1.8398407507610557 1103 68-69 -4.105787036894984 1103 70-71 -4.884613717106003 1103 72-73 -7.594450378459385 1103 74-75 -8.358982988977353 1103 76-77 -9.146656827663342 1103 78-79 -12.288150988813145 1103 80-81 -8.55715304726641 1103 82-83 -4.697969937731116 1103 84-85 -3.8311714475763274 1103 86-87 -3.582481881415468 1103 88-89 -3.680960985456448 1103 90-91 -9.158629078644065 1103 92-93 -14.69806094813288 1103 94-95 -12.762796307248932 1103 96-97 -12.740417151990869 1103 98-99 -12.208734282849383 1103 100-101 -11.82740573852218 1104 1 0.13980277292600007 1104 2 0.12411748275972556 1104 3 0.13261958108831706 1104 4 0.12873916740810643 1104 5 0.1489145343150895 1104 6 0.1700426618229045 1104 7 0.06262552322517934 1104 8 0.1915832842126548 1104 9 0.09851377049333365 1104 10-11 0.12689195413891952 1104 12-13 0.18137828961091174 1104 14-15 0.2744292026119979 1104 16-17 0.3008421471411964 1104 18-19 0.2488686729567533 1104 20-21 0.15023974706534915 1104 22-23 0.2089298443604264 1104 24-25 0.27955375381195324 1104 26-27 0.23100588029426916 1104 28-29 0.2523396779504665 1104 30-31 0.26683309691221524 1104 32-33 0.26845601002089126 1104 34-35 0.23572926588765597 1104 36-37 0.2177890228989341 1104 38-39 0.34776530175211917 1104 40-41 0.2349002593747329 1104 42-43 0.19892768746260003 1104 44-45 0.11863107751503321 1104 46-47 0.04477249084810353 1104 48-49 0.17617743233254402 1104 50-51 0.001331016466473045 1104 52-53 0.05806715955527153 1104 54-55 0.012265817433295467 1104 56-57 0.5690889768676684 1104 58-59 -6.776966027889504 1104 60-61 -8.716689912584417 1104 62-63 -2.642423652338554 1104 64-65 -0.33268780230932293 1104 66-67 -1.860027420947727 1104 68-69 -4.452317633425583 1104 70-71 -4.9316272141195014 1104 72-73 -7.923383982392988 1104 74-75 -8.64173139672576 1104 76-77 -9.141077622084136 1104 78-79 -12.38220163286379 1104 80-81 -8.70966599977936 1104 82-83 -4.982032021793202 1104 84-85 -3.984167850572728 1104 86-87 -3.778700077633662 1104 88-89 -3.912262891758356 1104 90-91 -9.587857757872744 1104 92-93 -15.060287185359119 1104 94-95 -12.5073592768119 1104 96-97 -12.181809368383085 1104 98-99 -11.702828101943203 1104 100-101 -11.277763513879954 1105 1 0.12667901243643342 1105 2 0.18003411969027638 1105 3 0.15878104939690374 1105 4 0.017431191090189202 1105 5 0.08077168202710538 1105 6 0.14320818638007893 1105 7 0.09997298910146668 1105 8 0.13810409827819825 1105 9 0.1664075069755384 1105 10-11 0.21282659052086927 1105 12-13 0.2082611924381439 1105 14-15 0.18398896855843816 1105 16-17 0.2264128681144939 1105 18-19 0.21004599243427435 1105 20-21 0.11685097712091164 1105 22-23 0.26934267264104506 1105 24-25 0.20386724405486945 1105 26-27 0.2394951765072264 1105 28-29 0.13333657204378113 1105 30-31 0.19393920264655407 1105 32-33 0.239929064247427 1105 34-35 0.16614227015752192 1105 36-37 -0.012299402592070408 1105 38-39 0.05472871055448536 1105 40-41 0.08859466801447269 1105 42-43 0.16674805463684095 1105 44-45 0.1688755811889564 1105 46-47 0.07988641050476986 1105 48-49 0.10555640877911543 1105 50-51 0.05927441225777841 1105 52-53 0.051151844364611065 1105 54-55 -0.08190241493076655 1105 56-57 0.534855944051138 1105 58-59 -6.68675894923809 1105 60-61 -8.966972648403935 1105 62-63 -2.8223290186753296 1105 64-65 -0.5339683667857109 1105 66-67 -1.8475159209610794 1105 68-69 -4.196520911923095 1105 70-71 -4.800336721422447 1105 72-73 -6.215632445217992 1105 74-75 -6.658965750068463 1105 76-77 -8.69460848210108 1105 78-79 -11.888425219306065 1105 80-81 -7.992448183356775 1105 82-83 -4.072083987168227 1105 84-85 -3.0813046573268323 1105 86-87 -2.8363259342157825 1105 88-89 -2.967374013792938 1105 90-91 -8.205967172204822 1105 92-93 -11.396409644742015 1105 94-95 -11.77394052801045 1105 96-97 -12.135371997492435 1105 98-99 -11.62327556369285 1105 100-101 -11.2251104760629 1106 1 0.054630420176685845 1106 2 0.11914375186742276 1106 3 0.04299463580299445 1106 4 0.10851971456700227 1106 5 0.06456184077669036 1106 6 -0.06077397822909347 1106 7 0.11086592345167645 1106 8 0.09687505742895297 1106 9 0.1280384166515418 1106 10-11 0.20160370933727023 1106 12-13 0.1280888007702785 1106 14-15 0.18561809647218297 1106 16-17 0.09647612807805217 1106 18-19 0.12034438085252219 1106 20-21 0.04456090918690592 1106 22-23 0.14403999516075316 1106 24-25 0.12508682330231835 1106 26-27 0.16356347772277502 1106 28-29 0.15281910583804148 1106 30-31 0.17416040769037267 1106 32-33 0.16208234120929177 1106 34-35 0.15733537962386634 1106 36-37 0.15363535134704875 1106 38-39 0.12924561113709387 1106 40-41 0.15084023631272458 1106 42-43 0.08042660893173093 1106 44-45 -0.027643171241841458 1106 46-47 -0.016287785629259588 1106 48-49 0.08900946403250032 1106 50-51 0.04651124449178212 1106 52-53 0.058055185983221236 1106 54-55 -0.23704886986550378 1106 56-57 0.6191033148412899 1106 58-59 -6.825335015185999 1106 60-61 -9.053157623952824 1106 62-63 -2.8055071593644634 1106 64-65 -0.37915950891012073 1106 66-67 -1.6648541951886031 1106 68-69 -4.1683989288266385 1106 70-71 -4.712637371346142 1106 72-73 -6.192676832321389 1106 74-75 -6.666758738908017 1106 76-77 -8.572467568891163 1106 78-79 -11.975217524067366 1106 80-81 -8.100351409481654 1106 82-83 -4.125423458779473 1106 84-85 -3.1587813315417215 1106 86-87 -3.0110975626379 1106 88-89 -3.1065783853787146 1106 90-91 -8.340956639571427 1106 92-93 -11.688564609689173 1106 94-95 -11.926019982761584 1106 96-97 -12.191416811034223 1106 98-99 -11.663121887932121 1106 100-101 -11.297894847293314 1107 1 0.032100475770768355 1107 2 0.05416807397345025 1107 3 3.7907092765010475E-4 1107 4 -0.04610038436590003 1107 5 -0.0675754211595887 1107 6 -0.03395823843231227 1107 7 0.0776596047452145 1107 8 0.002791160723631947 1107 9 -0.06971396037609168 1107 10-11 0.03940396706985183 1107 12-13 0.04933010670023208 1107 14-15 0.1588603538494482 1107 16-17 0.046638569108139905 1107 18-19 0.09571534252398095 1107 20-21 0.11954507620311006 1107 22-23 0.10205261064731985 1107 24-25 0.14872085526096868 1107 26-27 0.16470476177531168 1107 28-29 0.2148528342536693 1107 30-31 0.04314167142187131 1107 32-33 0.03488566765311418 1107 34-35 -0.02983612266935154 1107 36-37 -0.0322540340933557 1107 38-39 0.05480501468142762 1107 40-41 -0.10887606451494491 1107 42-43 -0.008085276573524425 1107 44-45 -0.07124493278976018 1107 46-47 -0.07340637590149157 1107 48-49 -0.08321497964735158 1107 50-51 -0.0030679578684313924 1107 52-53 -0.11657693573938843 1107 54-55 -0.25739633411648555 1107 56-57 0.5366946530489898 1107 58-59 -6.841100335616716 1107 60-61 -8.78721690489554 1107 62-63 -2.7941262539918696 1107 64-65 -0.5866801272725688 1107 66-67 -1.9688879842932572 1107 68-69 -4.254162494659305 1107 70-71 -4.8636929688565225 1107 72-73 -6.108448050355037 1107 74-75 -6.8216854030720775 1107 76-77 -9.167289461282667 1107 78-79 -12.398490505264046 1107 80-81 -8.500553322850024 1107 82-83 -4.508502624187905 1107 84-85 -3.567972072745608 1107 86-87 -3.3701063713563677 1107 88-89 -3.4721372652354887 1107 90-91 -8.776511623584273 1107 92-93 -11.936097099118761 1107 94-95 -12.072276252941297 1107 96-97 -12.574655468671276 1107 98-99 -11.932912934147305 1107 100-101 -11.416458751614126 1108 1 0.004227263376279211 1108 2 0.048076160528484735 1108 3 -0.014514930876206478 1108 4 0.04128828300528653 1108 5 -0.11498863298784556 1108 6 -0.0032911295648148098 1108 7 0.015640880929865375 1108 8 -0.06928212366330655 1108 9 0.07638888482799899 1108 10-11 0.07176038517381755 1108 12-13 -0.017717052977992864 1108 14-15 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2311 64-65 1.282295063402735 2311 66-67 -0.2540281600685006 2311 68-69 -3.404243278642678 2311 70-71 -0.8420665143052659 2311 72-73 -0.9587648556019985 2311 74-75 1.4499113608357774 2311 76-77 4.571988899059566 2311 78-79 5.331114731922174 2311 80-81 3.132882399892665 2311 82-83 1.6600142524271817 2311 84-85 1.2824250409921163 2311 86-87 1.2056366162610281 2311 88-89 1.4455054237393732 2311 90-91 2.820262927564521 2311 92-93 4.756777135407244 2311 94-95 8.736552688369976 2311 96-97 9.558222103149395 2311 98-99 9.944178417825265 2311 100-101 9.241348538528037 2312 1 -0.3165788501074296 2312 2 -0.3650549092214064 2312 3 -0.3432867932130641 2312 4 -0.24583717020880158 2312 5 -0.3065152739011694 2312 6 -0.3243438354169186 2312 7 -0.3150373944288276 2312 8 -0.3656078032949779 2312 9 -0.4488634176328503 2312 10-11 -0.3797427150013206 2312 12-13 -0.42803449753260026 2312 14-15 -0.5946165383909516 2312 16-17 -0.5784777502794611 2312 18-19 -0.4724082123415272 2312 20-21 -0.36928309893988853 2312 22-23 -0.410732194695278 2312 24-25 -0.4810035246454589 2312 26-27 -0.5674058382690319 2312 28-29 -0.76095965460879 2312 30-31 -0.4812765558563754 2312 32-33 -0.3525123860255235 2312 34-35 -0.4129961405729219 2312 36-37 -0.43455992106837726 2312 38-39 -0.4103190545712039 2312 40-41 -0.3335509580064908 2312 42-43 -0.31434233126886113 2312 44-45 -0.5062704884476119 2312 46-47 -0.4579548655127823 2312 48-49 -0.387912448163938 2312 50-51 -0.23962429314688904 2312 52-53 -0.25253312340345957 2312 54-55 -0.4077284994401964 2312 56-57 0.8838843217120527 2312 58-59 4.234652672427373 2312 60-61 4.001611632801389 2312 62-63 1.4064050193612765 2312 64-65 1.2229630163820673 2312 66-67 -0.2648263694604509 2312 68-69 -0.917084290457062 2312 70-71 0.19051691954937766 2312 72-73 6.992733941393237 2312 74-75 5.430297876819342 2312 76-77 5.636333474274796 2312 78-79 5.679456833007784 2312 80-81 3.259168066535757 2312 82-83 1.8290337712832923 2312 84-85 1.4046442834467179 2312 86-87 1.208713348589395 2312 88-89 1.4133980302690397 2312 90-91 2.99037926987387 2312 92-93 4.891113101083523 2312 94-95 8.842915679816155 2312 96-97 9.506580123929721 2312 98-99 10.079217790936395 2312 100-101 9.303029478237157 2313 1 -0.31190509083005935 2313 2 -0.22714678175755054 2313 3 -0.324501076032341 2313 4 -0.2856151529703084 2313 5 -0.3216184133262914 2313 6 -0.3404350799801392 2313 7 -0.35083116733994757 2313 8 -0.31968242464341756 2313 9 -0.3412007318717727 2313 10-11 -0.4034011797686716 2313 12-13 -0.4033218830410732 2313 14-15 -0.4406216100291829 2313 16-17 -0.4142709860442295 2313 18-19 -0.4101395209912013 2313 20-21 -0.48412147404286543 2313 22-23 -0.49892562012153974 2313 24-25 -0.4782902763212391 2313 26-27 -0.6519018411676711 2313 28-29 -0.723628129342643 2313 30-31 -0.6936523346575569 2313 32-33 -0.5187801808297579 2313 34-35 -0.5372541095053549 2313 36-37 -0.6735870708868035 2313 38-39 -0.8132693797644919 2313 40-41 -0.9731081980313121 2313 42-43 -0.715355218386577 2313 44-45 -0.6145004495923985 2313 46-47 -0.6098894469463687 2313 48-49 -0.6991118031976598 2313 50-51 -0.5766418807835336 2313 52-53 -0.698333200459544 2313 54-55 -0.6906421180047602 2313 56-57 0.310567311117083 2313 58-59 3.927739574406459 2313 60-61 3.683119514935857 2313 62-63 0.9272504486608497 2313 64-65 0.8222152598226558 2313 66-67 1.5445396318120963 2313 68-69 0.1553116850471099 2313 70-71 2.6488069434230894 2313 72-73 8.108336773424153 2313 74-75 9.746780359255876 2313 76-77 8.401729236385375 2313 78-79 6.072758935349793 2313 80-81 3.2251805459105576 2313 82-83 1.785413116736276 2313 84-85 1.3278059854974913 2313 86-87 1.199175460482838 2313 88-89 1.172909231967985 2313 90-91 2.6182372181017506 2313 92-93 4.513261354454482 2313 94-95 8.44680757374049 2313 96-97 9.238261992652133 2313 98-99 9.695150626156009 2313 100-101 8.841871068405233 2314 1 -0.3488041433508222 2314 2 -0.33294756653833346 2314 3 -0.3827535032168079 2314 4 -0.2848899219491372 2314 5 -0.41641312615820425 2314 6 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-0.3612452753629327 2314 70-71 2.470764056961091 2314 72-73 8.196675919948468 2314 74-75 9.65357833669077 2314 76-77 8.708285824609408 2314 78-79 6.271962191445663 2314 80-81 3.2594179367803022 2314 82-83 1.6506373853907697 2314 84-85 1.2838415133104206 2314 86-87 1.216084668364676 2314 88-89 1.328338362435133 2314 90-91 2.6695465153082267 2314 92-93 4.768500935904729 2314 94-95 8.750075898584438 2314 96-97 9.421874111028549 2314 98-99 9.70966215860531 2314 100-101 8.881733784937737 2315 1 -0.7115448321101319 2315 2 -0.5147538917673877 2315 3 -0.5556237784665186 2315 4 -0.3978191103796789 2315 5 -0.6687679653346237 2315 6 -0.7010301178564333 2315 7 -0.6863066011167831 2315 8 -0.7128733702440044 2315 9 -1.0914850285218591 2315 10-11 -0.8629772385991359 2315 12-13 -0.7088526998004099 2315 14-15 -0.7733845046279413 2315 16-17 -0.9330674086945905 2315 18-19 -0.8934978175654678 2315 20-21 -0.8892884160283927 2315 22-23 -0.9136411638663162 2315 24-25 -0.9862814810806597 2315 26-27 -1.0239304174698987 2315 28-29 -1.0200651940445695 2315 30-31 -0.8733528555688181 2315 32-33 -0.7927729477244583 2315 34-35 -0.9314493205532202 2315 36-37 -0.9880650274223299 2315 38-39 -1.0070483954796146 2315 40-41 -0.8800497496326471 2315 42-43 -0.8959013223254289 2315 44-45 -0.8530126703582255 2315 46-47 -0.9382941237758899 2315 48-49 -0.9147989182749541 2315 50-51 -0.7191235062421484 2315 52-53 -0.6629390655493097 2315 54-55 -0.6629708766558622 2315 56-57 0.5061796412124338 2315 58-59 3.842279253241024 2315 60-61 3.4750620740446188 2315 62-63 0.9671239266762406 2315 64-65 0.8065251022724382 2315 66-67 1.7739667042349225 2315 68-69 6.010066521581525 2315 70-71 5.002207530370981 2315 72-73 8.816539504866562 2315 74-75 9.960593759902675 2315 76-77 8.64384576509335 2315 78-79 5.936336818666465 2315 80-81 3.0862526535163397 2315 82-83 1.1669123626020053 2315 84-85 0.687935774399751 2315 86-87 0.7007480698472719 2315 88-89 0.8710141454121221 2315 90-91 2.2339561295149153 2315 92-93 4.2481444786053295 2315 94-95 8.313036697887352 2315 96-97 8.912018070116483 2315 98-99 9.147195417342614 2315 100-101 8.470440263217263 2316 1 -0.4687734287490457 2316 2 -0.5041085753416681 2316 3 -0.6367126845484918 2316 4 -0.4293998440701756 2316 5 -0.5123514580283768 2316 6 -0.5181672829765347 2316 7 -0.6314209270100548 2316 8 -0.5948116308554603 2316 9 -0.6990046505335457 2316 10-11 -0.6256806562921113 2316 12-13 -0.5992463889656889 2316 14-15 -0.7611871474967842 2316 16-17 -1.0179253102950057 2316 18-19 -1.0004875560521853 2316 20-21 -0.8255074502312709 2316 22-23 -0.8972077797647273 2316 24-25 -2.219178519653177 2316 26-27 -1.444005998101403 2316 28-29 -1.1493547466513476 2316 30-31 -0.8667846111934736 2316 32-33 -0.6509418721997875 2316 34-35 -0.7684027305417587 2316 36-37 -0.6947199062840852 2316 38-39 -1.0027007553924818 2316 40-41 -0.8013558116542683 2316 42-43 -0.9183422494867699 2316 44-45 -0.718161723035557 2316 46-47 -0.7253429878159068 2316 48-49 -0.9662775031544513 2316 50-51 -0.9138944773431774 2316 52-53 -0.7991644457840792 2316 54-55 -0.8215063098161224 2316 56-57 0.28001710088572906 2316 58-59 3.711488144335817 2316 60-61 3.690549493898093 2316 62-63 0.9856047677424051 2316 64-65 0.6623137579193106 2316 66-67 5.742886455519134 2316 68-69 8.725833430411146 2316 70-71 5.476622073448574 2316 72-73 9.089004559113985 2316 74-75 9.951419688856312 2316 76-77 8.540494147889238 2316 78-79 5.928219878937956 2316 80-81 2.8551251035469143 2316 82-83 1.4907804778670268 2316 84-85 1.1236381037381236 2316 86-87 1.072139347114934 2316 88-89 1.0548478420709877 2316 90-91 2.4028270792056823 2316 92-93 4.291786423726958 2316 94-95 8.311790300730376 2316 96-97 9.101142796203094 2316 98-99 9.418550727004641 2316 100-101 8.557385664635206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 26.0 4 80.0 5 184.0 6 419.0 7 1061.0 8 2487.0 9 4207.0 10 7126.0 11 10974.0 12 15223.0 13 20745.0 14 27003.0 15 37250.0 16 49819.0 17 63408.0 18 66627.0 19 65308.0 20 66388.0 21 65728.0 22 62775.0 23 61278.0 24 65137.0 25 71944.0 26 93445.0 27 133938.0 28 184597.0 29 240426.0 30 288929.0 31 314143.0 32 327200.0 33 340168.0 34 347152.0 35 338274.0 36 286247.0 37 178544.0 38 56547.0 39 2543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73588364925705 20.380140138886503 15.010459217520673 22.873516994335777 2 15.33126123728921 21.440266695336962 40.172011512937175 23.056460554436654 3 18.560788008670805 26.263619978979868 30.604231328209675 24.571360684139655 4 12.543179948031394 17.726390193549314 37.92448361747063 31.805946240948664 5 12.487883961279001 38.91636974080471 34.86864352489363 13.727102773022656 6 30.43699927540546 38.05504352698961 17.622401055896415 13.885556141708525 7 27.245722930409432 33.883946360517 21.39985780092283 17.470472908150736 8 25.931274292159696 35.82379607876946 19.93714700355216 18.307782625518687 9 26.439637364128938 16.541355119744093 20.503813742301507 36.515193773825466 10-11 24.331937599699078 27.364842240361597 27.944466410920437 20.358753749018884 12-13 25.374298915186795 24.893369935953967 28.31263937344141 21.419691775417828 14-15 22.298352416414872 26.173632607232 26.710536714234106 24.81747826211902 16-17 21.184673289794382 29.422533704285957 27.436288167892414 21.956504838027247 18-19 21.16216504011115 27.8989273329322 30.076848172899407 20.86205945405724 20-21 22.277273831751963 26.942234896351447 30.035731944219577 20.744759327677016 22-23 21.78336168163366 26.900411638360705 29.98601871577966 21.330207964225973 24-25 21.398972584726096 27.156239119217584 30.308840384743185 21.135947911313142 26-27 21.139244501258037 27.365186462787754 30.12901600217173 21.366553033782477 28-29 21.112872888150136 27.56770806805645 30.139370833054823 21.180048210738583 30-31 21.38751336762888 27.322657192730578 30.000493968769625 21.289335470870917 32-33 21.14540202943598 27.4470093568971 29.871894644012237 21.53569396965468 34-35 21.440454159116058 27.395108808954888 30.014939782221724 21.14949724970733 36-37 21.489870119737358 27.095698902515565 29.930823178130026 21.48360779961705 38-39 21.06349456072186 27.461174869706017 29.983092002553143 21.492238567018983 40-41 21.36867214091721 27.33684401260477 30.031424668827007 21.263059177651016 42-43 21.20990308958881 27.329600883304302 29.691174579643807 21.76932144746308 44-45 21.253967598066755 27.502669875564933 29.730107085045127 21.51325544132318 46-47 21.44983674408559 27.555157502206967 29.289893558658108 21.705112195049335 48-49 21.219191025937665 27.691999897849357 29.23056054652134 21.858248529691636 50-51 21.28175791392599 27.790588778179604 29.01168029594566 21.91597301194875 52-53 21.38306640419237 27.79837385187277 28.869999898627196 21.948559845307663 54-55 21.379828907169244 27.764719770370682 28.918217010995157 21.937234311464913 56-57 21.3483251461297 27.77478101425383 28.819783436364034 22.057110403252437 58-59 19.915844176391413 27.490967008657684 30.45434915605692 22.13883965889398 60-61 21.264950832747694 27.76813116826611 28.915695210264275 22.051222788721923 62-63 21.00126522224523 27.810860179214586 29.018260921105398 22.169613677434782 64-65 20.459222385209177 27.617552949991097 29.387228999927284 22.53599566487244 66-67 21.004290660536892 27.301726581993226 29.293500202907634 22.40048255456225 68-69 21.854567866241723 26.571557579932303 29.474755531239598 22.099119022586375 70-71 20.940677971658616 26.82715112760311 29.754952135964157 22.477218764774115 72-73 20.661816025410918 26.90823956064319 29.743576541867128 22.686367872078765 74-75 20.97933614876222 27.0598442810363 29.149669596234563 22.811149973966923 76-77 19.26831173090315 27.39777634557246 29.965367614931882 23.368544308592508 78-79 19.990176203559987 27.725945707873066 28.927228804412024 23.356649284154923 80-81 20.483657340148913 27.61540802381929 28.313383004742203 23.5875516312896 82-83 20.609837453654407 27.505009814361042 28.210720104686516 23.674432627298035 84-85 20.419631018749264 27.452212752118264 28.21083030082601 23.91732592830646 86-87 20.42182777925175 27.45769200570453 28.190323237849007 23.93015697719471 88-89 20.409470275013614 27.319815182229473 28.14028240966143 24.130432133095482 90-91 20.1558453914971 27.268803406715197 28.286870723284107 24.288480478503597 92-93 19.951934630480604 27.16879526609699 28.445260969434372 24.434009133988035 94-95 19.121560953297966 26.899156813902632 29.208387421207128 24.77089481159228 96-97 18.85172823270926 26.78140715170107 29.494398187944388 24.87246642764528 98-99 18.976365762258418 26.840850275468263 28.961913799972145 25.220870162301175 100-101 18.693122474784392 26.904171476831163 29.14411637079674 25.25858967758771 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 883.0 1 655.0 2 469.5 3 561.5 4 599.5 5 679.0 6 928.5 7 1392.0 8 2031.0 9 2632.5 10 2994.5 11 3327.5 12 3788.0 13 4294.0 14 4840.0 15 5379.0 16 5928.5 17 6445.0 18 7074.5 19 7940.0 20 8913.5 21 10347.0 22 12192.5 23 14327.0 24 17295.0 25 21296.5 26 26466.5 27 32471.0 28 38782.5 29 45538.5 30 53004.5 31 61946.0 32 72833.5 33 83563.5 34 93009.5 35 102153.0 36 111275.5 37 118557.0 38 123497.5 39 127662.5 40 131697.0 41 135807.0 42 139503.5 43 142926.5 44 146110.5 45 147900.0 46 148109.0 47 148432.0 48 147978.5 49 144958.0 50 140450.5 51 134617.0 52 128017.0 53 121609.5 54 113512.0 55 103008.5 56 91990.5 57 80726.5 58 69414.5 59 58486.0 60 47649.0 61 37226.5 62 27927.0 63 20437.5 64 14749.5 65 10675.5 66 7728.5 67 5409.0 68 3724.0 69 2597.5 70 1825.0 71 1334.5 72 1003.0 73 769.5 74 562.5 75 375.0 76 237.5 77 137.5 78 78.0 79 53.0 80 28.5 81 14.5 82 10.5 83 5.0 84 2.0 85 1.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02027016798324401 2 0.004413251763440468 3 0.003515206346461303 4 0.0 5 0.06984227500049392 6 2.5658440485119E-5 7 0.0 8 1.2829220242559501E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.30925964366071E-4 16-17 0.0 18-19 0.001488189548136902 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7960908339583303E-4 28-29 0.0022707719829330314 30-31 0.008864991187608615 32-33 3.5921816679166605E-4 34-35 0.0010391668396473198 36-37 0.02651799824137049 38-39 0.024940004151535668 40-41 0.02696702094986007 42-43 0.027557165081017812 44-45 0.02832691829557138 46-47 0.030007546147346673 48-49 0.029417402016188938 50-51 0.024683419746684483 52-53 0.021668552989682996 54-55 0.016370085029505925 56-57 0.15697833888795806 58-59 0.748495196611649 60-61 0.30770884751778965 62-63 3.5921816679166605E-4 64-65 0.1392226980722557 66-67 1.3976537408851597 68-69 2.6861949636073508 70-71 2.1740524915243755 72-73 1.8249180918433614 74-75 1.5376333629517265 76-77 1.1073413160162808 78-79 0.40954719780322696 80-81 1.7960908339583303E-4 82-83 7.6975321455357E-5 84-85 1.28292202425595E-5 86-87 0.002540185608026781 88-89 1.28292202425595E-5 90-91 0.21971322587407402 92-93 0.16960229160663662 94-95 1.2687585651081645 96-97 1.2435234888910498 98-99 0.9754184442620415 100-101 0.3946909607623431 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3897353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.671905351117836 #Duplication Level Percentage of deduplicated Percentage of total 1 66.10405669523438 27.54681993928734 2 15.082372490139631 12.570223977588041 3 6.8596744630917454 8.575671148963178 4 3.622610395702132 6.03844310134699 5 2.168440957668793 4.518153317373064 6 1.4147104139809252 3.5372207082392295 7 0.96210936646312 2.8065051319672554 8 0.6811644034317995 2.270833483868847 9 0.5264735353664771 1.9745239802094199 >10 2.3607466207404784 16.62463774695377 >50 0.13064076897232638 3.7954080750216166 >100 0.08021408635271662 6.234272136359046 >500 0.0050585076044635325 1.4802108102921943 >1k 0.0015422279026318536 0.9502860737646868 >5k 6.168911610527409E-5 0.2563505039112008 >10k+ 1.2337823221054818E-4 0.8204398648541891 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18025 0.46249338974427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13890 0.3563957383383029 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9972 0.2558659685176067 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.5658440485119002E-5 0.0 0.0 0.0 0.0 2 5.1316880970238005E-5 0.0 0.0 0.0 0.0 3 5.1316880970238005E-5 0.0 0.0 0.0 0.0 4 5.1316880970238005E-5 0.0 0.0 0.0 0.0 5 7.6975321455357E-5 0.0 0.0 0.0 0.0 6 1.0263376194047601E-4 0.0 0.0 0.0 0.0 7 1.7960908339583303E-4 0.0 0.0 0.0 0.0 8 1.7960908339583303E-4 0.0 0.0 0.0 0.0 9 1.7960908339583303E-4 0.0 0.0 0.0 0.0 10-11 1.9243830363839252E-4 0.0 0.0 2.5658440485119002E-5 0.0 12-13 2.1809674412351151E-4 0.0 0.0 6.414610121279751E-5 0.0 14-15 2.82242845336309E-4 0.0 0.0 1.411214226681545E-4 0.0 16-17 3.07901285821428E-4 0.0 0.0 1.53950642910714E-4 0.0 18-19 3.7204738703422555E-4 0.0 0.0 1.667798631532735E-4 0.0 20-21 4.1053504776190404E-4 0.0 0.0 4.2336426800446353E-4 0.0 22-23 4.1053504776190404E-4 0.0 0.0 9.750207384345221E-4 0.0 24-25 6.15802571642856E-4 0.0 0.0 0.0021296505602648773 0.0 26-27 6.15802571642856E-4 0.0 0.0 0.004015545935921124 0.0 28-29 6.927778930982131E-4 0.0 0.0 0.010968983307388373 0.0 30-31 7.184363335833321E-4 0.0 1.2829220242559501E-5 0.03606293810183476 0.0 32-33 7.184363335833321E-4 0.0 2.5658440485119002E-5 0.09071541633513823 0.0 34-35 7.569239943110105E-4 0.0 2.5658440485119002E-5 0.16825522348116784 0.0 36-37 8.467285360089271E-4 0.0 2.5658440485119002E-5 0.2923394416672034 0.0 38-39 9.750207384345221E-4 0.0 2.5658440485119002E-5 0.481454977262773 0.0 40-41 9.750207384345221E-4 0.0 2.5658440485119002E-5 0.6943430579678054 0.0 42-43 0.0011033129408601172 0.0 2.5658440485119002E-5 0.9088732788638854 0.0 44-45 0.001231605143285712 0.0 2.5658440485119002E-5 1.159325316439132 0.0 46-47 0.001436872667166664 0.0 2.5658440485119002E-5 1.4450192220206894 0.0 48-49 0.001513847988622021 0.0 2.5658440485119002E-5 1.7240804207368436 0.0 50-51 0.001513847988622021 0.0 2.5658440485119002E-5 2.0056689758407824 0.0 52-53 0.0015266772088645807 0.0 2.5658440485119002E-5 2.27311973023742 0.0 54-55 0.0015395064291071402 0.0 2.5658440485119002E-5 2.5596731935752293 0.0 56-57 0.001565164869592259 2.5658440485119002E-5 2.5658440485119002E-5 2.8097531837634415 0.0 58-59 0.001565164869592259 7.6975321455357E-5 2.5658440485119002E-5 2.9865013510451837 0.0 60-61 0.001565164869592259 7.6975321455357E-5 2.5658440485119002E-5 3.1201433383119257 0.0 62-63 0.0016164817505624973 7.6975321455357E-5 2.5658440485119002E-5 3.2358115880188425 0.0 64-65 0.0016164817505624973 8.98045416979165E-5 2.5658440485119002E-5 3.353866072690875 0.0 66-67 0.0016164817505624973 1.0263376194047601E-4 2.5658440485119002E-5 3.451445121855783 2.5658440485119002E-5 68-69 0.0016164817505624973 1.0263376194047601E-4 2.5658440485119002E-5 3.563444214573327 2.5658440485119002E-5 70-71 0.0016934570720178541 1.0263376194047601E-4 2.5658440485119002E-5 3.7692633949247094 2.5658440485119002E-5 72-73 0.0017062862922604138 1.0263376194047601E-4 2.5658440485119002E-5 4.023961904400243 2.5658440485119002E-5 74-75 0.0017576031732306516 1.0263376194047601E-4 2.5658440485119002E-5 4.3161858830852635 2.5658440485119002E-5 76-77 0.0017704323934732112 1.0263376194047601E-4 2.5658440485119002E-5 4.731172670271335 2.5658440485119002E-5 78-79 0.0017704323934732112 1.0263376194047601E-4 2.5658440485119002E-5 5.313901512128873 2.5658440485119002E-5 80-81 0.0019243830363839254 1.0263376194047601E-4 2.5658440485119002E-5 5.956016814489219 2.5658440485119002E-5 82-83 0.001975699917354163 1.0263376194047601E-4 2.5658440485119002E-5 6.568342667446341 2.5658440485119002E-5 84-85 0.0020013583578392825 1.1546298218303552E-4 2.5658440485119002E-5 7.065410805744309 2.5658440485119002E-5 86-87 0.0020526752388095202 1.2829220242559501E-4 2.5658440485119002E-5 7.505632669147496 2.5658440485119002E-5 88-89 0.002078333679294639 1.2829220242559501E-4 2.5658440485119002E-5 7.954988937363385 2.5658440485119002E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9385 0.0 45.78871 1 GTATCAA 16820 0.0 39.851295 1 ATCAACG 21320 0.0 31.143879 3 TATCAAC 21425 0.0 31.058876 2 TCAACGC 21420 0.0 30.974874 4 CAACGCA 21900 0.0 30.27444 5 AACGCAG 22255 0.0 29.813229 6 ACGCAGA 25655 0.0 25.678467 7 CGCAGAG 25665 0.0 25.576658 8 GTACATG 19820 0.0 25.441835 1 TACATGG 19480 0.0 25.347626 2 ACATGGG 19535 0.0 24.236925 3 GCAGAGT 27585 0.0 23.198246 9 GAGTACT 14755 0.0 21.206062 12-13 AGAGTAC 24690 0.0 20.784288 10-11 CATGGGG 8510 0.0 20.557926 4 CATGGGA 10365 0.0 20.245367 4 AGTACTT 15735 0.0 20.24469 12-13 GTACTTT 15870 0.0 19.760826 14-15 CAGAGTA 27115 0.0 19.264355 10-11 >>END_MODULE