FastQCFastQC Report
Fri 27 May 2016
SRR939886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139400
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175650.4243368604145529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124180.2999951683818911No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86990.21015122964680874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96750.046.4371341
GTATCAA183000.042.7517781
TATCAAC228750.034.016742
ATCAACG229900.033.8690953
TCAACGC230200.033.7844854
CAACGCA236400.032.859425
AACGCAG241100.032.3172156
ACGCAGA274350.027.9717587
CGCAGAG276500.027.5670748
TACATGG202550.025.7583222
GTACATG205350.025.65881
GCAGAGT286600.025.1837259
ACATGGG209250.024.2155723
GAGTACT153650.022.61335812-13
AGAGTAC251200.021.60441410-11
CATGGGG98000.021.3160484
GTACTTT164350.021.16080714-15
AGTACTT162250.021.12477112-13
CAGAGTA280500.020.17796110-11
GAGTACA156400.019.8527281
Produced by FastQC (version 0.11.5)