FastQCFastQC Report
Fri 27 May 2016
SRR939886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139400
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188710.45588732666570037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152250.36780692854036817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106860.2581533555587766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105600.047.866771
GTATCAA179500.038.6044041
TATCAAC230600.029.8165842
ATCAACG230800.029.7218823
TCAACGC231900.029.5399884
CAACGCA239550.028.596635
AACGCAG244200.028.05216
GTACATG211500.025.4702931
TACATGG207150.025.38072
ACATGGG207300.024.2119733
ACGCAGA280900.024.0999457
CGCAGAG281250.023.968758
GCAGAGT302700.021.706079
GAGTACT160250.021.40372312-13
CATGGGA108050.021.1185514
AGTACTT169000.020.73064812-13
CATGGGG92700.020.3167674
AGAGTAC264100.020.2801910-11
GTACTTT172250.019.73358714-15
CAGAGTA293900.018.9502510-11
Produced by FastQC (version 0.11.5)