##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939886_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4139400 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37227279315843 31.0 31.0 34.0 30.0 34.0 2 31.600182152002706 31.0 31.0 34.0 30.0 34.0 3 31.636524617094263 31.0 31.0 34.0 30.0 34.0 4 35.45950210175388 37.0 35.0 37.0 33.0 37.0 5 35.192011885780545 37.0 35.0 37.0 32.0 37.0 6 35.22298932212398 37.0 35.0 37.0 32.0 37.0 7 35.12549016765715 37.0 35.0 37.0 32.0 37.0 8 35.088078223897185 37.0 35.0 37.0 32.0 37.0 9 36.66034497753298 39.0 35.0 39.0 32.0 39.0 10-11 36.522259143837275 38.0 35.0 39.0 32.0 39.0 12-13 36.444895757839305 38.0 35.0 39.0 32.0 39.0 14-15 37.4460401265884 39.0 36.0 41.0 32.0 41.0 16-17 37.37637519930425 39.0 36.0 41.0 32.0 41.0 18-19 37.386299101319025 39.0 36.0 41.0 32.0 41.0 20-21 37.317539256897135 39.0 36.0 40.5 31.0 41.0 22-23 37.185542711504084 39.0 36.0 40.0 31.0 41.0 24-25 37.03485831279896 39.0 36.0 40.0 31.0 41.0 26-27 36.73703954679422 39.0 35.5 40.0 30.0 41.0 28-29 36.511467845581485 38.0 35.0 40.0 30.0 41.0 30-31 36.23457578393004 38.0 35.0 40.0 30.0 41.0 32-33 36.18583043436247 38.0 35.0 40.0 30.0 41.0 34-35 36.02813934386626 38.0 35.0 40.0 30.0 41.0 36-37 36.05256764265353 38.0 35.0 40.0 30.0 41.0 38-39 35.95179675798425 38.0 35.0 40.0 29.0 41.0 40-41 35.86570408754892 38.0 35.0 40.0 29.0 41.0 42-43 35.622795091076 38.0 34.5 40.0 27.5 41.0 44-45 35.34306843987051 38.0 34.0 40.0 27.0 41.0 46-47 35.029955911484755 38.0 34.0 40.0 26.0 41.0 48-49 34.69621261535488 38.0 33.0 40.0 24.5 41.0 50-51 34.46692588297821 38.0 33.0 40.0 24.0 41.0 52-53 34.22233874474561 38.0 33.0 40.0 24.0 41.0 54-55 33.9651637918539 37.5 33.0 40.0 23.0 41.0 56-57 33.600364062424504 37.0 32.5 40.0 22.5 41.0 58-59 33.23832741460115 37.0 32.0 39.5 22.0 41.0 60-61 32.933112890757116 36.0 31.0 39.0 21.0 41.0 62-63 32.64348746195101 36.0 31.0 39.0 20.0 40.0 64-65 32.21396265159201 35.5 31.0 39.0 18.5 40.0 66-67 31.33255278542784 35.0 29.5 38.0 12.0 40.0 68-69 29.492300454172103 34.0 26.5 37.5 7.5 40.0 70-71 28.847132434652366 33.5 25.5 37.0 7.5 39.0 72-73 29.379279243368604 34.0 27.0 36.0 7.5 39.0 74-75 29.457194400154613 34.0 28.0 36.0 7.5 39.0 76-77 28.98422947770208 32.5 27.5 35.0 7.0 37.5 78-79 29.51184386626081 34.0 29.0 35.0 7.0 37.5 80-81 29.324275136493213 34.0 29.0 35.0 3.5 37.0 82-83 28.879871962120113 33.5 28.5 35.0 2.0 36.5 84-85 28.51609919311978 33.0 28.0 35.0 2.0 36.0 86-87 28.137895347151762 33.0 27.0 35.0 2.0 36.0 88-89 27.67633147316036 33.0 26.0 35.0 2.0 35.0 90-91 27.1896760400058 33.0 26.0 35.0 2.0 35.0 92-93 26.711352611489588 32.0 25.0 34.5 2.0 35.0 94-95 25.920624849011936 32.0 24.0 34.0 2.0 35.0 96-97 25.390928516210078 32.0 21.5 34.0 2.0 35.0 98-99 24.725903150215007 31.5 19.0 34.0 2.0 35.0 100-101 23.236104991061506 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2533135769174777 1101 2 0.3305712674726671 1101 3 0.2829161697082938 1101 4 0.25971265758844453 1101 5 0.2732067069051851 1101 6 0.14444207580257995 1101 7 0.23293340043273503 1101 8 0.20884502345175093 1101 9 0.46480694201446937 1101 10-11 0.4064326996419112 1101 12-13 0.3590817559304966 1101 14-15 0.2668675737054258 1101 16-17 0.32025043059189073 1101 18-19 0.258450634219642 1101 20-21 0.3001903866538598 1101 22-23 0.30487179037399414 1101 24-25 0.25503505694805284 1101 26-27 0.2196398314460879 1101 28-29 0.24038386110836285 1101 30-31 0.09354286306298576 1101 32-33 0.10707581314593995 1101 34-35 0.13804060754126368 1101 36-37 0.2316585665973605 1101 38-39 0.4175228688408694 1101 40-41 0.3273799588395647 1101 42-43 0.13106040264447927 1101 44-45 0.006030789716895413 1101 46-47 -0.09009239579490469 1101 48-49 -0.1283519899931207 1101 50-51 -0.1309842115730504 1101 52-53 -0.27759678401886134 1101 54-55 -0.4254625059355348 1101 56-57 -0.47282983385930066 1101 58-59 -0.36450439108229205 1101 60-61 -0.5117624433368633 1101 62-63 -0.37838247385673185 1101 64-65 -0.5348195509821885 1101 66-67 -0.5811888715407427 1101 68-69 -9.230239051489715 1101 70-71 -6.901315342478558 1101 72-73 -2.053436899019456 1101 74-75 -0.970223184315639 1101 76-77 -0.7260699333427212 1101 78-79 -0.850764622806345 1101 80-81 -0.9585616295931594 1101 82-83 -0.7447063575918627 1101 84-85 -0.6796185587727699 1101 86-87 -0.8681166887189704 1101 88-89 -0.7380669118307068 1101 90-91 -0.8910456556053248 1101 92-93 -1.1582709122588497 1101 94-95 -0.8912305637280831 1101 96-97 -1.2927022947328624 1101 98-99 -1.2495720224609173 1101 100-101 -1.1770192328618947 1102 1 0.18343322828829756 1102 2 0.10875928352540143 1102 3 0.15261792494646542 1102 4 0.13049444809054478 1102 5 0.35999761724572465 1102 6 0.2197232467054988 1102 7 0.23517873690247626 1102 8 0.23681729083334346 1102 9 0.25828559607348467 1102 10-11 0.32915598272173696 1102 12-13 0.2765353454199442 1102 14-15 0.39886299828330607 1102 16-17 0.386187035622477 1102 18-19 0.2798377870709885 1102 20-21 0.21958997748068754 1102 22-23 0.322204418497698 1102 24-25 0.2363662727576994 1102 26-27 0.2956814195216708 1102 28-29 0.18200265826947515 1102 30-31 0.14945038465594251 1102 32-33 0.06128296390956223 1102 34-35 -0.07234063715545602 1102 36-37 -0.003907330539874465 1102 38-39 0.25451144113760193 1102 40-41 0.29735252387681754 1102 42-43 0.18240232062174044 1102 44-45 0.12185249243124474 1102 46-47 0.19423677053156752 1102 48-49 0.1933552460364396 1102 50-51 0.1742934401719083 1102 52-53 0.13734294587930407 1102 54-55 -0.09119759912345415 1102 56-57 -0.17786632509861278 1102 58-59 -0.18945367076594977 1102 60-61 0.03591303913917443 1102 62-63 0.04255552126709006 1102 64-65 0.027278342587308657 1102 66-67 0.2963494051157163 1102 68-69 -4.594104030028536 1102 70-71 0.2395268111427633 1102 72-73 0.5903069450379235 1102 74-75 0.22932644356312082 1102 76-77 0.08662191502652661 1102 78-79 -0.05303785157434859 1102 80-81 0.031759394094233784 1102 82-83 0.07139503033096517 1102 84-85 -0.0622131057832398 1102 86-87 -0.15909156007075254 1102 88-89 -0.3426369983678832 1102 90-91 -0.37969100974837033 1102 92-93 -0.3925179003040462 1102 94-95 -0.17124960128747446 1102 96-97 -0.7451534192614453 1102 98-99 -0.3114716081201152 1102 100-101 -0.27438912736983667 1103 1 0.24068069270360581 1103 2 0.2319399663994588 1103 3 0.17926760237127226 1103 4 0.1643217156248653 1103 5 0.29052600222207303 1103 6 0.1780934105012122 1103 7 0.16849289685224278 1103 8 0.27175177155364594 1103 9 0.3165278558348845 1103 10-11 0.2776157588853252 1103 12-13 0.33588136939545166 1103 14-15 0.3833804703398016 1103 16-17 0.34916884767128664 1103 18-19 0.33060037650189145 1103 20-21 0.2874202858274728 1103 22-23 0.18002543139920846 1103 24-25 0.3044820068306606 1103 26-27 0.3439491904890559 1103 28-29 0.22227130024207753 1103 30-31 0.15871752349824675 1103 32-33 0.2761271275531456 1103 34-35 0.043239537085362656 1103 36-37 0.11411667331005049 1103 38-39 0.27821551015382795 1103 40-41 0.32220071939958217 1103 42-43 0.2599385951057158 1103 44-45 0.32636642602903265 1103 46-47 0.21638858470319633 1103 48-49 0.16741841827523984 1103 50-51 0.023451070739419322 1103 52-53 0.19377906296587355 1103 54-55 0.07150938890369929 1103 56-57 0.21633055911883758 1103 58-59 0.06653287439085176 1103 60-61 -0.015406264066584185 1103 62-63 -0.10655748395446807 1103 64-65 -0.1178886970240356 1103 66-67 0.2008964985583681 1103 68-69 -4.143056157801819 1103 70-71 0.30722528176299235 1103 72-73 0.6562674756252669 1103 74-75 0.31944444314451914 1103 76-77 0.18815768937850663 1103 78-79 0.020612394837598913 1103 80-81 0.12836950839378503 1103 82-83 0.04997181923499738 1103 84-85 -0.001030178032426221 1103 86-87 0.09118780665078319 1103 88-89 -0.04437155778689572 1103 90-91 -0.16894643078642702 1103 92-93 -0.13037403927511804 1103 94-95 -0.20202673481525935 1103 96-97 -0.46049028873345677 1103 98-99 -0.07777033059607774 1103 100-101 -0.20485111186972205 1104 1 0.14566358551297398 1104 2 0.17495840861070988 1104 3 0.18439459501343336 1104 4 0.1348716742637066 1104 5 0.19294196562961474 1104 6 0.22515378286807675 1104 7 0.05565989175128294 1104 8 0.18649673395544397 1104 9 0.17732198413239786 1104 10-11 0.1682429110911059 1104 12-13 0.1426557631507137 1104 14-15 0.22780964006265236 1104 16-17 0.24962983578357267 1104 18-19 0.20948209373485582 1104 20-21 0.30883745558973175 1104 22-23 0.17257851205183528 1104 24-25 0.18058946643747475 1104 26-27 0.22933555088909685 1104 28-29 0.14405413788333732 1104 30-31 0.16678508413914273 1104 32-33 0.24787003697265675 1104 34-35 0.2060653625696247 1104 36-37 0.23472715865512583 1104 38-39 0.24117767372310794 1104 40-41 0.11151267329719161 1104 42-43 0.09370437720181002 1104 44-45 0.10492197073804022 1104 46-47 0.1399488625857046 1104 48-49 0.12654744270414398 1104 50-51 0.0360606569158648 1104 52-53 0.030792420038210366 1104 54-55 -0.01772655957142888 1104 56-57 0.0033348558432919617 1104 58-59 0.014170183440661788 1104 60-61 0.01606977258200004 1104 62-63 0.07450561278587031 1104 64-65 0.17659526765284284 1104 66-67 0.32257595568978914 1104 68-69 -4.109609861185437 1104 70-71 0.6300324312879866 1104 72-73 0.8649316758473127 1104 74-75 0.5794501868378781 1104 76-77 0.47244304585618124 1104 78-79 0.10621491991327048 1104 80-81 0.06654581410634108 1104 82-83 -0.010367351231195698 1104 84-85 0.09211085501440763 1104 86-87 0.2116838957747582 1104 88-89 0.064769601836133 1104 90-91 0.05537861807526667 1104 92-93 -0.003345693073701028 1104 94-95 0.23114351225610008 1104 96-97 -0.10909390552880538 1104 98-99 0.03658063354733443 1104 100-101 -0.1397835443626647 1105 1 0.201232646219637 1105 2 0.17254113054080022 1105 3 0.13960074437810022 1105 4 0.10743588780174207 1105 5 0.15763356873712553 1105 6 0.17607738834604447 1105 7 0.11074345811326225 1105 8 0.21379931182674738 1105 9 0.30190933553614485 1105 10-11 0.18214273512100476 1105 12-13 0.16036447172711377 1105 14-15 0.19149414475907633 1105 16-17 0.22985777624227666 1105 18-19 0.17829029150555442 1105 20-21 0.18370716255675035 1105 22-23 0.306677492427319 1105 24-25 0.19729384098341285 1105 26-27 0.2656110490607233 1105 28-29 0.3835561001616341 1105 30-31 0.22484899834659444 1105 32-33 0.29885903732943575 1105 34-35 0.3298687529096682 1105 36-37 0.12850160074696504 1105 38-39 0.3656842533808984 1105 40-41 0.3877618061887418 1105 42-43 0.2714900737782173 1105 44-45 0.20689608404136095 1105 46-47 0.3154286337986605 1105 48-49 0.35739827586254336 1105 50-51 0.327108642243914 1105 52-53 0.22565309093610608 1105 54-55 0.248109065174269 1105 56-57 0.18229791939899798 1105 58-59 0.28923642059168486 1105 60-61 0.21900286679640857 1105 62-63 0.32367150413016077 1105 64-65 0.3378422797461482 1105 66-67 0.9396200035032045 1105 68-69 2.079453212477535 1105 70-71 2.3431364592496955 1105 72-73 1.3761031426017531 1105 74-75 0.8160247564367715 1105 76-77 0.5629588015298559 1105 78-79 0.5158157865465895 1105 80-81 0.5361305436678485 1105 82-83 0.500201135002623 1105 84-85 0.3607135383641129 1105 86-87 0.2906101585135836 1105 88-89 0.555627795157907 1105 90-91 0.49569097580685195 1105 92-93 0.36666725632171904 1105 94-95 0.66142591965151 1105 96-97 0.28382979843022227 1105 98-99 0.4348136760159953 1105 100-101 0.4294815536907777 1106 1 0.3454358048700321 1106 2 0.299684785322647 1106 3 0.35192725789807966 1106 4 0.295859404193223 1106 5 0.30205981704214224 1106 6 0.32903214227441424 1106 7 0.2715898527850342 1106 8 0.292492629606123 1106 9 0.4631084361981834 1106 10-11 0.33185334172545566 1106 12-13 0.38206262020761983 1106 14-15 0.5783571552480282 1106 16-17 0.5588040951383348 1106 18-19 0.4674456416690731 1106 20-21 0.4339676415389633 1106 22-23 0.4121964064418435 1106 24-25 0.5738617001203679 1106 26-27 0.6027263128798097 1106 28-29 0.4831220100683069 1106 30-31 0.5998327963046322 1106 32-33 0.6500152399696262 1106 34-35 0.5634299118791191 1106 36-37 0.6753298640874732 1106 38-39 0.6318233566352589 1106 40-41 0.4402034539114865 1106 42-43 0.48466657134541435 1106 44-45 0.6703162353933578 1106 46-47 0.6797390250405826 1106 48-49 0.7396194013311614 1106 50-51 0.679324832321285 1106 52-53 0.5964474147376535 1106 54-55 0.6714472341939697 1106 56-57 0.7121532974798797 1106 58-59 0.7735459892684062 1106 60-61 0.774537858221791 1106 62-63 0.6253229907298277 1106 64-65 0.7829971214148088 1106 66-67 1.2023747494551849 1106 68-69 2.6919502213313287 1106 70-71 3.0516274509143706 1106 72-73 2.0015075704951855 1106 74-75 1.5501548563410559 1106 76-77 1.0937420580904345 1106 78-79 0.8385561002181277 1106 80-81 0.8487358569405643 1106 82-83 0.90204293327972 1106 84-85 0.9911493234402862 1106 86-87 0.9557114586651352 1106 88-89 1.001970371133023 1106 90-91 0.9819603684047635 1106 92-93 0.9032097156421344 1106 94-95 1.2375250412515761 1106 96-97 1.0897480304634009 1106 98-99 1.3775450661013409 1106 100-101 1.454855820859958 1107 1 0.13490882299294427 1107 2 0.10761116872741283 1107 3 0.09164031179433962 1107 4 0.13236139956968884 1107 5 0.13363418875869826 1107 6 0.18805401407534106 1107 7 0.13748867215213778 1107 8 0.18663063119230117 1107 9 0.1276797114954462 1107 10-11 0.15537589833846965 1107 12-13 0.14806333525267235 1107 14-15 0.2271061252175457 1107 16-17 0.1303789098031629 1107 18-19 0.052438800579224676 1107 20-21 0.12891462133489995 1107 22-23 0.1346441455776528 1107 24-25 0.13286908821616095 1107 26-27 0.10998987342451017 1107 28-29 0.19227189743289586 1107 30-31 0.13934817574958203 1107 32-33 0.1367242433500948 1107 34-35 0.2564059476749705 1107 36-37 0.21828398097519397 1107 38-39 0.25077895845430476 1107 40-41 0.1569465643082566 1107 42-43 0.0950074446425262 1107 44-45 0.11475233061457857 1107 46-47 0.20262837656432708 1107 48-49 0.301980540565971 1107 50-51 0.27517971543919373 1107 52-53 0.21654694802486318 1107 54-55 0.14610830846303458 1107 56-57 0.22797024172745495 1107 58-59 0.33271043064736716 1107 60-61 0.27092721466718217 1107 62-63 0.2948833223926073 1107 64-65 0.15640074993449105 1107 66-67 0.7536917119831692 1107 68-69 2.1157757756494426 1107 70-71 2.31803754155705 1107 72-73 1.4295083516309326 1107 74-75 0.8829516422240822 1107 76-77 0.4240110507276498 1107 78-79 0.46656138114575896 1107 80-81 0.5208707374992123 1107 82-83 0.6218470446833173 1107 84-85 0.4477025144958269 1107 86-87 0.4300471768881664 1107 88-89 0.6052463953981508 1107 90-91 0.5336652622096736 1107 92-93 0.47504985983233894 1107 94-95 0.9477719286611581 1107 96-97 0.5232806553607503 1107 98-99 0.8109608897601248 1107 100-101 0.9790721715568615 1108 1 -0.16985654769643332 1108 2 -0.18560631930663973 1108 3 -0.14749650245327928 1108 4 -0.08543400586030714 1108 5 -0.08473836963063519 1108 6 -0.0630372402388204 1108 7 -0.09764344348270271 1108 8 -0.058395582695929704 1108 9 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2316 70-71 1.2724695991359383 2316 72-73 0.3579289168239299 2316 74-75 -0.26223490204663236 2316 76-77 -0.6663256766653589 2316 78-79 -0.7725340283504636 2316 80-81 -0.8429867409690885 2316 82-83 -0.8040635860961594 2316 84-85 -0.8232310010186517 2316 86-87 -0.8841160270889397 2316 88-89 -0.7795467399638802 2316 90-91 -0.8131125753504804 2316 92-93 -0.8443313302077229 2316 94-95 -0.5824661705046914 2316 96-97 -0.6269820945170572 2316 98-99 -0.6401150184860569 2316 100-101 -0.7146854541325851 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 38.0 4 66.0 5 184.0 6 457.0 7 1142.0 8 2476.0 9 4380.0 10 7240.0 11 11191.0 12 15635.0 13 20283.0 14 24914.0 15 31289.0 16 38831.0 17 45215.0 18 41054.0 19 33109.0 20 32219.0 21 33566.0 22 36150.0 23 41339.0 24 48633.0 25 60029.0 26 74049.0 27 91124.0 28 112042.0 29 135344.0 30 161767.0 31 191574.0 32 229836.0 33 276394.0 34 343130.0 35 433291.0 36 550113.0 37 615790.0 38 365672.0 39 29828.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80540369436494 20.417111175278755 15.020690038456289 22.756795091900017 2 15.382037274380966 21.3956048629516 40.11113495289958 23.11122290976785 3 18.60209658455249 26.24516264317598 30.62901160289211 24.52372916937942 4 12.590327100545974 17.725467459052034 37.922186790356086 31.7620186500459 5 12.531816205247138 38.925254867855244 34.8288640865826 13.714064840315022 6 30.467732151454836 38.00979320911079 17.64683713746851 13.875637501965866 7 27.286442479586416 33.86749287336329 21.403585060636807 17.44247958641349 8 25.9448712373774 35.82195487268686 19.878243223655602 18.354930666280136 9 26.45538000676427 16.53894284195777 20.503937768758757 36.501739382519204 10-11 24.305720635840945 27.39046721747113 27.962881093878337 20.340931052809587 12-13 25.397968304585206 24.90066193168092 28.288206020196167 21.413163743537712 14-15 22.305660240614582 26.17988114219452 26.705549113398074 24.80890950379282 16-17 21.158196485274136 29.39826312320867 27.456271271280947 21.987269120236245 18-19 21.17454220418418 27.920326617384163 30.07806686959463 20.82706430883703 20-21 22.288073150698168 26.906882639996134 30.064888631202592 20.740155578103106 22-23 21.77163357008262 26.923660433879306 29.977363869159785 21.32734212687829 24-25 21.371689465064854 27.148663368764403 30.316840681461244 21.162806484709495 26-27 21.16672547164877 27.36849899447708 30.125838635344326 21.33893689852982 28-29 21.11205099583961 27.56485154297251 30.173818816341946 21.149278644845936 30-31 21.39285009384249 27.295263569474 30.019184018487778 21.29270231819573 32-33 21.103996424537815 27.47903341728422 29.918066351397872 21.49890380678009 34-35 21.483001526361996 27.402763879674936 30.004911513040756 21.10932308092231 36-37 21.505067954199124 27.085965934090783 29.945141144480193 21.463824967229904 38-39 21.015668042973864 27.46770800299402 30.0175295698902 21.499094384141923 40-41 21.371213028973468 27.299224743964313 30.050484684651792 21.279077542410434 42-43 21.20749717999077 27.3072415322036 29.708744344111622 21.77651694369401 44-45 21.27231258732432 27.458900442660838 29.758555667272645 21.510231302742195 46-47 21.444399591219067 27.538925285286464 29.284803836050944 21.73187128744353 48-49 21.21516859522457 27.69163400685666 29.2142579852767 21.878939412642076 50-51 21.31103244666575 27.759562445142432 29.043634587531525 21.885770520660298 52-53 21.439413865361352 27.75874139736648 28.874049806069056 21.927794931203106 54-55 21.372112757390944 27.781198826532606 28.912123013688394 21.934565402388053 56-57 21.284346536474274 27.864063076580663 28.730140855145176 22.121449531799886 58-59 21.201531161425518 27.653927364303684 28.89409998359435 22.250441490676444 60-61 21.15312482672468 27.88829345600048 28.81241641258993 22.146165304684907 62-63 21.046487048009034 27.821323524970857 28.97687424882076 22.155315178199345 64-65 20.88995726818367 27.673534963081526 28.873214851948603 22.563292916786203 66-67 20.73227909839397 27.791850049740844 28.81554130425526 22.660329547609923 68-69 20.825003037834318 27.548597466458308 29.052173864535145 22.574225631172233 70-71 20.78237382375715 27.434446506549953 28.871158499928583 22.91202116976431 72-73 20.736223769860832 27.439327621767646 28.768424590853027 23.0560240175185 74-75 20.655182188103442 27.643604513338214 28.435454058420728 23.265759240137616 76-77 20.574072838944097 27.533858403196042 28.494633789869596 23.397434967990264 78-79 20.5855660459295 27.704154301631128 28.37293225017494 23.337347402264427 80-81 20.651477782826888 27.610915548675237 28.205804640978048 23.531802027519827 82-83 20.641250249432407 27.522045476177937 28.223004891049374 23.613699383340283 84-85 20.447769631367766 27.425355421327286 28.20901085983104 23.917864087473905 86-87 20.43819161615455 27.464240490694987 28.179086420137832 23.918481473012633 88-89 20.39305061940882 27.344279308753304 28.133465542813607 24.12920452902426 90-91 20.27280704986082 27.219934905592574 28.266083808596594 24.241174235950012 92-93 20.375297846326156 27.084187443332798 28.144282401440435 24.396232308900615 94-95 19.963242040429403 26.822104676050536 28.556950298180933 24.657702985339125 96-97 20.064304936939408 26.644543068650727 28.55547297496471 24.735679019445154 98-99 19.9844876577813 26.7488836912609 28.238733013743644 25.027895637214158 100-101 19.800702322030922 26.643818066967878 28.470316813218314 25.085162797782885 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1521.0 1 1033.0 2 720.0 3 925.0 4 823.0 5 832.5 6 1407.0 7 2755.0 8 4361.5 9 5208.5 10 4831.5 11 4377.5 12 4645.5 13 4969.0 14 5316.5 15 5757.0 16 6282.0 17 6789.5 18 7330.5 19 7995.0 20 9044.0 21 10464.5 22 12267.0 23 14800.5 24 17936.0 25 21763.0 26 27505.0 27 35000.0 28 41143.0 29 47369.0 30 55583.0 31 64699.0 32 77033.5 33 87869.5 34 95947.5 35 104707.0 36 116257.5 37 124802.5 38 126270.5 39 129012.0 40 134019.5 41 138208.0 42 139399.5 43 143410.5 44 149208.5 45 151988.5 46 152050.5 47 151654.0 48 153366.0 49 151868.5 50 146863.0 51 141114.0 52 135103.5 53 130415.0 54 125192.5 55 114925.5 56 102573.0 57 90733.5 58 80432.5 59 70545.5 60 58886.5 61 47574.0 62 36865.5 63 26740.0 64 19093.0 65 14134.0 66 10228.0 67 7501.0 68 5316.5 69 3618.0 70 2636.0 71 1910.0 72 1416.0 73 1160.0 74 907.5 75 675.0 76 462.5 77 271.5 78 138.5 79 77.0 80 42.0 81 25.5 82 21.5 83 14.5 84 7.5 85 3.5 86 2.5 87 2.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022587814659129343 2 0.02435135526887955 3 2.4158090544523364E-5 4 0.0 5 0.0 6 2.4158090544523364E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.8652944871237383E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.4158090544523364E-5 26-27 2.7781804126201864E-4 28-29 8.455331690583176E-5 30-31 4.106875392568972E-4 32-33 0.001811856790839252 34-35 0.003527081219500411 36-37 0.10029231289558874 38-39 0.09219935256317341 40-41 0.10111368797410251 42-43 0.1019109049620718 44-45 0.09552109001304536 46-47 0.08910711697347441 48-49 0.08963859496545393 50-51 0.0891433541092912 52-53 0.06947866840604919 54-55 0.05869208097791951 56-57 0.031864521428226315 58-59 0.014035850606368073 60-61 0.009530366719814465 62-63 0.001811856790839252 64-65 0.005133594240711214 66-67 0.1443687490940716 68-69 0.3961202106585495 70-71 0.3812871430642122 72-73 0.17111175532685896 74-75 0.028434072570903995 76-77 0.031018988259167997 78-79 0.005036961878533121 80-81 0.0013770111610378315 82-83 4.831618108904673E-5 84-85 4.106875392568972E-4 86-87 0.0011233512103203363 88-89 2.2950186017297195E-4 90-91 6.643474899743925E-4 92-93 0.0024399671449968595 94-95 0.07617045948688216 96-97 0.016475817751364932 98-99 0.018360148813837754 100-101 0.010037686621249457 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4139400.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.128392188212594 #Duplication Level Percentage of deduplicated Percentage of total 1 65.98871560768883 27.140097755094516 2 15.003008552500905 12.3409923950073 3 6.669874349513435 8.229636242786636 4 3.6989192703209217 6.085224096891841 5 2.254613638251943 4.636431697345939 6 1.4432334402803053 3.561472257059501 7 1.0156375400975999 2.9240077349143903 8 0.7190733011959909 2.3659462994929146 9 0.5407122260788266 2.001476204561831 >10 2.4451944990762198 16.998444823351168 >50 0.13219294631656148 3.7851420003108003 >100 0.08166193189447778 6.28755485350512 >500 0.005166535464673399 1.4695704724087937 >1k 0.0018200294381436066 1.0926639934480136 >5k 0.0 0.0 >10k+ 1.7613188111066975E-4 1.0813391738213207 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18871 0.45588732666570037 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15225 0.36780692854036817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10686 0.2581533555587766 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.415809054452336E-5 0.0 0.0 0.0 0.0 2 2.415809054452336E-5 0.0 0.0 0.0 0.0 3 2.415809054452336E-5 0.0 0.0 0.0 0.0 4 2.415809054452336E-5 0.0 0.0 0.0 0.0 5 4.831618108904672E-5 0.0 0.0 0.0 0.0 6 9.663236217809344E-5 0.0 0.0 0.0 0.0 7 1.932647243561869E-4 0.0 0.0 0.0 0.0 8 1.932647243561869E-4 0.0 0.0 0.0 0.0 9 1.932647243561869E-4 0.0 0.0 0.0 0.0 10-11 1.932647243561869E-4 0.0 0.0 0.0 0.0 12-13 2.1742281490071024E-4 0.0 0.0 2.415809054452336E-5 0.0 14-15 2.65738995989757E-4 0.0 0.0 9.663236217809344E-5 0.0 16-17 3.5029231289558877E-4 0.0 0.0 1.811856790839252E-4 0.0 18-19 4.710827656182055E-4 0.0 0.0 2.7781804126201864E-4 0.0 20-21 5.073199014349906E-4 0.0 0.0 3.986084939846355E-4 0.0 22-23 5.073199014349906E-4 0.0 0.0 9.78402667053196E-4 0.0 24-25 7.005846257911774E-4 0.0 0.0 0.0017997777455669903 1.207904527226168E-5 26-27 7.126636710634391E-4 0.0 0.0 0.0033096584045997 2.415809054452336E-5 28-29 8.092960332415326E-4 0.0 0.0 0.010412137024689568 2.415809054452336E-5 30-31 8.213750785137943E-4 0.0 0.0 0.036285451997874085 2.415809054452336E-5 32-33 8.213750785137943E-4 0.0 0.0 0.09055660240614583 2.415809054452336E-5 34-35 9.05928395419626E-4 0.0 0.0 0.1672585398850075 2.415809054452336E-5 36-37 0.0010146398028699811 0.0 0.0 0.28967966371937964 2.415809054452336E-5 38-39 0.0010871140745035513 0.0 0.0 0.4773034739334203 2.415809054452336E-5 40-41 0.0010871140745035513 0.0 0.0 0.6882760786587427 2.415809054452336E-5 42-43 0.0011837464366816447 0.0 0.0 0.8996593709233223 2.415809054452336E-5 44-45 0.0012924578441319999 0.0 0.0 1.1475334589554043 2.415809054452336E-5 46-47 0.00150988065903271 0.0 0.0 1.4347248393486978 2.415809054452336E-5 48-49 0.001594433975938542 0.0 0.0 1.7452650142532735 2.415809054452336E-5 50-51 0.001594433975938542 0.0 0.0 2.0794559598009372 2.415809054452336E-5 52-53 0.001594433975938542 0.0 0.0 2.4003720345943855 2.415809054452336E-5 54-55 0.001594433975938542 0.0 0.0 2.739612021065855 2.415809054452336E-5 56-57 0.0016669082475721118 0.0 0.0 3.1197154176933855 2.415809054452336E-5 58-59 0.0016669082475721118 0.0 0.0 3.5072715852539016 2.415809054452336E-5 60-61 0.0016669082475721118 0.0 0.0 3.9064115572305163 2.415809054452336E-5 62-63 0.0016669082475721118 0.0 0.0 4.285741894960623 2.415809054452336E-5 64-65 0.0016669082475721118 0.0 0.0 4.682876745422042 2.415809054452336E-5 66-67 0.001811856790839252 0.0 0.0 5.103263758032565 2.415809054452336E-5 68-69 0.0018360148813837754 0.0 0.0 5.567352756438131 2.415809054452336E-5 70-71 0.001884331062472822 0.0 0.0 6.098577088466928 2.415809054452336E-5 72-73 0.0019447262888341306 0.0 0.0 6.636988452432719 2.415809054452336E-5 74-75 0.0019809634246509156 0.0 0.0 7.181777552302266 2.415809054452336E-5 76-77 0.0019809634246509156 0.0 0.0 7.75277818041262 2.415809054452336E-5 78-79 0.0019809634246509156 0.0 0.0 8.382591679953617 2.415809054452336E-5 80-81 0.0023070976470019808 0.0 0.0 9.05004348456298 2.415809054452336E-5 82-83 0.0023191766922742428 0.0 0.0 9.719113881238826 2.415809054452336E-5 84-85 0.0024037300091800744 0.0 0.0 10.371104507899695 2.415809054452336E-5 86-87 0.002488283326085906 0.0 0.0 11.020461902691212 2.415809054452336E-5 88-89 0.002488283326085906 0.0 0.0 11.712373773976903 2.415809054452336E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10560 0.0 47.86677 1 GTATCAA 17950 0.0 38.604404 1 TATCAAC 23060 0.0 29.816584 2 ATCAACG 23080 0.0 29.721882 3 TCAACGC 23190 0.0 29.539988 4 CAACGCA 23955 0.0 28.59663 5 AACGCAG 24420 0.0 28.0521 6 GTACATG 21150 0.0 25.470293 1 TACATGG 20715 0.0 25.3807 2 ACATGGG 20730 0.0 24.211973 3 ACGCAGA 28090 0.0 24.099945 7 CGCAGAG 28125 0.0 23.96875 8 GCAGAGT 30270 0.0 21.70607 9 GAGTACT 16025 0.0 21.403723 12-13 CATGGGA 10805 0.0 21.118551 4 AGTACTT 16900 0.0 20.730648 12-13 CATGGGG 9270 0.0 20.316767 4 AGAGTAC 26410 0.0 20.28019 10-11 GTACTTT 17225 0.0 19.733587 14-15 CAGAGTA 29390 0.0 18.95025 10-11 >>END_MODULE