FastQCFastQC Report
Fri 27 May 2016
SRR939885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101727
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76400.6934567274833058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57850.5250847079176603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41330.37513830558750033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22710.2061309199102863No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.12925162041050098No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.10719533968033823No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11100.10075091197728657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28450.057.3579441
GTATCAA57350.043.921641
TATCAAC74650.034.6960682
TGGTATC11650.034.6107032
ATCAACG76400.033.901333
TCAACGC77650.033.355594
CAACGCA79100.032.62425
AACGCAG80800.032.1726346
GTGGTAT13700.030.4704991
ACGCAGA91200.028.0356967
GTACATG65700.027.8701521
TACATGG67150.027.8334882
CGCAGAG92450.027.5001968
ACATGGG69900.026.1955533
GCAGAGT93800.025.4862299
TAAGGTG4750.023.968185
GAGTACT57850.023.5318712-13
GTATAGG2704.1836756E-1122.840051
AGTACTT60000.022.53053512-13
CATGGGG25250.022.3564574
Produced by FastQC (version 0.11.5)