##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939885_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1101727 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.867952768698597 31.0 30.0 33.0 16.0 34.0 2 29.70676129385955 31.0 30.0 33.0 25.0 34.0 3 29.95506055492876 31.0 30.0 34.0 25.0 34.0 4 33.756695624233586 35.0 33.0 37.0 28.0 37.0 5 33.7319136228848 35.0 35.0 37.0 28.0 37.0 6 33.80009748331483 35.0 35.0 37.0 28.0 37.0 7 33.71416149372757 35.0 33.0 37.0 28.0 37.0 8 33.588711177996004 35.0 33.0 37.0 28.0 37.0 9 34.84191818844414 37.0 34.0 39.0 28.0 39.0 10-11 34.666796765441894 37.0 34.0 39.0 27.0 39.0 12-13 34.52214613965165 37.0 34.0 39.0 27.0 39.0 14-15 35.12878780314905 38.0 33.5 40.0 26.5 41.0 16-17 34.96682481231739 38.0 33.0 40.0 26.0 41.0 18-19 34.87854159878083 38.0 33.0 40.0 25.0 41.0 20-21 35.0953162625587 38.0 34.0 40.0 26.0 41.0 22-23 35.001144566666696 38.0 34.0 40.0 25.5 41.0 24-25 34.778617570414454 38.0 33.5 40.0 24.5 41.0 26-27 34.616536583019204 38.0 33.0 40.0 24.5 41.0 28-29 34.62639065757669 38.0 33.0 40.0 24.0 41.0 30-31 34.34905244221119 38.0 33.0 40.0 24.0 41.0 32-33 33.92646181858119 38.0 33.0 40.0 22.0 41.0 34-35 33.69083992676952 37.5 32.5 40.0 21.0 41.0 36-37 33.420635511338105 37.0 32.0 40.0 19.0 41.0 38-39 33.15652108008608 37.0 31.5 40.0 18.0 41.0 40-41 32.85967304059899 37.0 31.0 40.0 16.0 41.0 42-43 32.39361021378254 37.0 31.0 40.0 14.5 41.0 44-45 31.956409800249972 36.5 30.0 39.5 9.0 41.0 46-47 31.463270846589037 36.0 30.0 39.0 8.0 41.0 48-49 31.009486469878652 36.0 29.0 39.0 4.5 40.0 50-51 30.348459736395675 35.0 28.0 38.5 2.0 40.0 52-53 30.48340968316107 35.0 28.0 39.0 2.0 40.0 54-55 30.54849341079959 35.0 28.0 39.0 2.0 40.0 56-57 30.244167112179333 35.0 28.0 39.0 2.0 40.0 58-59 29.832750309287146 35.0 27.5 39.0 2.0 40.0 60-61 29.401933056011153 34.5 26.5 38.0 2.0 40.0 62-63 28.93901846827753 34.0 26.0 38.0 2.0 40.0 64-65 28.38545438207469 34.0 25.5 38.0 2.0 40.0 66-67 27.7838693251595 33.5 23.5 37.0 2.0 39.0 68-69 27.21965922592439 33.0 22.5 36.5 2.0 39.0 70-71 26.642092823358237 32.5 21.5 36.0 2.0 39.0 72-73 25.97711728949186 32.0 20.0 35.0 2.0 38.0 74-75 25.62538496378867 32.0 18.5 35.0 2.0 38.0 76-77 25.063326032674155 31.0 16.5 35.0 2.0 37.0 78-79 24.394892745662037 31.0 9.5 35.0 2.0 36.5 80-81 23.665164782201035 30.5 6.5 34.0 2.0 36.0 82-83 22.920506622784046 29.5 2.0 34.0 2.0 35.5 84-85 22.123804717502612 29.0 2.0 34.0 2.0 35.0 86-87 21.23406388333952 29.0 2.0 34.0 2.0 35.0 88-89 20.267227271365776 26.5 2.0 33.5 2.0 35.0 90-91 18.904767242701688 25.0 2.0 32.5 2.0 34.5 92-93 17.446507619401174 20.0 2.0 32.0 2.0 34.0 94-95 16.88622589806731 19.0 2.0 31.0 2.0 34.0 96-97 16.49507364347066 17.5 2.0 31.0 2.0 34.0 98-99 15.57803702732165 2.0 2.0 31.0 2.0 34.0 100-101 13.971688993734382 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7133018089535277 1101 2 1.0695526979509786 1101 3 1.1600657519284034 1101 4 1.0512034384077182 1101 5 0.9948534329732368 1101 6 1.0794740747981848 1101 7 1.2487816477132085 1101 8 1.0446781963140026 1101 9 1.2536507643764878 1101 10-11 1.2145114301899014 1101 12-13 1.2462969448524746 1101 14-15 1.4351076403769625 1101 16-17 1.0392471065286841 1101 18-19 1.145240311829447 1101 20-21 0.9797014029133351 1101 22-23 1.2359667923960913 1101 24-25 1.2694843366264692 1101 26-27 0.7639844539139489 1101 28-29 1.0894237464789782 1101 30-31 0.8887457975962576 1101 32-33 0.9598631390078296 1101 34-35 0.9963836945033577 1101 36-37 1.0288584350241265 1101 38-39 1.2275428218067077 1101 40-41 1.0501074131077885 1101 42-43 1.3615310835686216 1101 44-45 1.4261191405066818 1101 46-47 1.5480725470916106 1101 48-49 1.6469914626009086 1101 50-51 1.8358982323162003 1101 52-53 1.2849506409243254 1101 54-55 1.2403327078565702 1101 56-57 1.0976531246286996 1101 58-59 0.8071482673502679 1101 60-61 1.3734738294842437 1101 62-63 1.1132972121496572 1101 64-65 1.1407400712610034 1101 66-67 1.269360718967775 1101 68-69 1.2879078544498732 1101 70-71 0.7723676049796921 1101 72-73 0.849194578907948 1101 74-75 0.8241098997652152 1101 76-77 0.7287520472307953 1101 78-79 0.659415812702683 1101 80-81 0.05599200946829441 1101 82-83 0.04900483843139014 1101 84-85 -0.14323990844981438 1101 86-87 -0.35058877186480686 1101 88-89 -3.7554172802876415 1101 90-91 -7.143219112623537 1101 92-93 -9.54732086501942 1101 94-95 -7.66658998437968 1101 96-97 -4.7520002807545225 1101 98-99 -3.2416015190572907 1101 100-101 -2.4600201533574815 1102 1 1.8415249243789766 1102 2 1.2414205881375402 1102 3 1.1137050284337882 1102 4 1.0374104003370377 1102 5 0.9512987158490134 1102 6 1.2211536971248975 1102 7 1.2083931675069337 1102 8 1.0771605639766761 1102 9 1.2583053222408864 1102 10-11 1.2802155861128455 1102 12-13 1.4024545172777678 1102 14-15 1.6052359962089042 1102 16-17 1.3376412366667054 1102 18-19 1.5132347756547162 1102 20-21 1.3668046753234648 1102 22-23 1.3520700458467232 1102 24-25 1.3261590294825325 1102 26-27 1.518572653519584 1102 28-29 1.3858982269886155 1102 30-31 1.6373783434782538 1102 32-33 1.7850423063064795 1102 34-35 1.674740474421462 1102 36-37 1.8739829989555759 1102 38-39 1.3569532760763892 1102 40-41 1.5665500416532154 1102 42-43 1.8615097541283063 1102 44-45 1.7883475456333322 1102 46-47 1.6265008991316527 1102 48-49 1.9394725978959144 1102 50-51 1.8211998780010141 1102 52-53 1.7334187131221093 1102 54-55 1.9468561986801767 1102 56-57 2.107234783205982 1102 58-59 1.7197568105020942 1102 60-61 1.7741876547534723 1102 62-63 1.6355793674659047 1102 64-65 1.4397456453547406 1102 66-67 1.5184791021014021 1102 68-69 1.4969387394794502 1102 70-71 1.41596924687261 1102 72-73 1.575402773204928 1102 74-75 1.3400927603749508 1102 76-77 0.7688276956457791 1102 78-79 0.7811500383965999 1102 80-81 1.0304061608409647 1102 82-83 1.0405654898798957 1102 84-85 1.001257569936353 1102 86-87 0.9292108261937386 1102 88-89 -0.2935590594473574 1102 90-91 -5.680891644096313 1102 92-93 -10.11264109101669 1102 94-95 -8.398552387686218 1102 96-97 -5.288869403308746 1102 98-99 -3.8189894683974988 1102 100-101 -2.808540838174711 1103 1 2.1182905397018885 1103 2 1.3931002166287207 1103 3 1.0317175842252446 1103 4 0.8874836462628082 1103 5 1.0677448829502794 1103 6 0.8767801497595755 1103 7 0.7012434052990884 1103 8 0.9078859157513719 1103 9 1.1611933371048977 1103 10-11 1.0440666077849556 1103 12-13 1.0899915116123537 1103 14-15 1.0848882551772476 1103 16-17 1.021213695284473 1103 18-19 0.9139728340549169 1103 20-21 0.7884978103176508 1103 22-23 0.9037129942409123 1103 24-25 0.6628686076728414 1103 26-27 0.7012001139999313 1103 28-29 0.5778871109766683 1103 30-31 0.8549021741144784 1103 32-33 0.7622795065523817 1103 34-35 0.8800638877501612 1103 36-37 0.8432014272191353 1103 38-39 0.8486214091851423 1103 40-41 0.7825918135418632 1103 42-43 0.8468817085947151 1103 44-45 0.7849280693017029 1103 46-47 0.8977656425880873 1103 48-49 0.8900127437478709 1103 50-51 0.8956004840796297 1103 52-53 0.9844476983975063 1103 54-55 0.8712340653840158 1103 56-57 0.5343242346499864 1103 58-59 0.9640536838951981 1103 60-61 1.0403983150637757 1103 62-63 0.9326070226592797 1103 64-65 1.058251497030028 1103 66-67 1.0059758740243296 1103 68-69 0.6998505221321629 1103 70-71 0.6658047006431183 1103 72-73 0.6652964352041195 1103 74-75 0.5177375553434906 1103 76-77 0.3448057744314319 1103 78-79 0.1753662384299659 1103 80-81 -0.09906715410280853 1103 82-83 -0.45041017784641824 1103 84-85 -0.4507463517487835 1103 86-87 -0.3267968252059319 1103 88-89 -0.9616458895785165 1103 90-91 -6.5075341925517485 1103 92-93 -9.620522741141128 1103 94-95 -8.152870583528227 1103 96-97 -5.183938044225453 1103 98-99 -3.7581519280893865 1103 100-101 -2.814023998467878 1104 1 1.8369860194798413 1104 2 1.2120280268983485 1104 3 0.9395065414020891 1104 4 0.6759978463024581 1104 5 0.7682498145521848 1104 6 0.7378211142718669 1104 7 0.6731648713974181 1104 8 0.650517012103478 1104 9 0.6462083301659618 1104 10-11 0.9142236012425258 1104 12-13 0.7758199711682607 1104 14-15 0.938499910113805 1104 16-17 0.8642059881076278 1104 18-19 0.9664779763031319 1104 20-21 0.7583403831764954 1104 22-23 0.695485706869782 1104 24-25 0.7124736458369938 1104 26-27 0.8488117565455227 1104 28-29 0.5581120688474002 1104 30-31 0.5969694818067879 1104 32-33 0.7280785995341432 1104 34-35 0.5790317208191524 1104 36-37 0.7418312968662377 1104 38-39 0.6215189731224982 1104 40-41 0.9080227091604129 1104 42-43 1.0604414454107314 1104 44-45 0.9639892062961515 1104 46-47 0.6824064286705571 1104 48-49 0.6119791270745942 1104 50-51 0.5068480678425153 1104 52-53 0.527775476450639 1104 54-55 0.6144692210144669 1104 56-57 0.6774639798918543 1104 58-59 0.4503842870871111 1104 60-61 0.29434142816845466 1104 62-63 0.39171004109702423 1104 64-65 0.29016441336626997 1104 66-67 0.45153588344145845 1104 68-69 0.48840127550250045 1104 70-71 0.2672278023481134 1104 72-73 0.18905888811847404 1104 74-75 0.2727735510810021 1104 76-77 0.22914267223079676 1104 78-79 0.1038648258605761 1104 80-81 -0.25599401026854807 1104 82-83 -0.36520157604229553 1104 84-85 -0.29831803344981367 1104 86-87 -0.18952791660164792 1104 88-89 -0.5661286289718568 1104 90-91 -2.944411546834065 1104 92-93 -7.635232049229947 1104 94-95 -5.171030773853365 1104 96-97 -2.52009238601768 1104 98-99 -1.6677939532678163 1104 100-101 -0.7438400546732709 1105 1 1.9705574480512702 1105 2 1.1328985626126347 1105 3 0.82213154140209 1105 4 0.7983951677310301 1105 5 0.6779417788378979 1105 6 0.5378255785575874 1105 7 0.6570354071117066 1105 8 0.8171687978177644 1105 9 0.793962794451673 1105 10-11 0.630522708385385 1105 12-13 0.42138247116826477 1105 14-15 0.8276583922566658 1105 16-17 0.6667997381076276 1105 18-19 0.6879132441602778 1105 20-21 0.565561365319347 1105 22-23 0.5296040104412043 1105 24-25 0.8083508779798478 1105 26-27 0.743548363688376 1105 28-29 0.5498241224188334 1105 30-31 0.6185944818067881 1105 32-33 1.0080830638198606 1105 34-35 0.6162727922477202 1105 36-37 0.563860314723378 1105 38-39 0.8917354909796344 1105 40-41 0.7017258341604204 1105 42-43 0.7790865346964466 1105 44-45 0.6539490277247246 1105 46-47 0.6483930358134167 1105 48-49 0.5191465377888775 1105 50-51 0.6441895856996602 1105 52-53 0.6606348514506415 1105 54-55 0.5635272567287508 1105 56-57 0.4717988013204284 1105 58-59 0.5907168763728237 1105 60-61 0.531513303168456 1105 62-63 0.17389307681131427 1105 64-65 -0.3062016580623066 1105 66-67 0.028033651298599693 1105 68-69 -0.1412415816403545 1105 70-71 -0.032428447651884795 1105 72-73 0.2567218345470472 1105 74-75 0.14160614036671504 1105 76-77 -0.1347836670549185 1105 78-79 -0.2555860669965675 1105 80-81 -0.29380874241140376 1105 82-83 -0.5353645224708679 1105 84-85 -0.47765062273553127 1105 86-87 -0.497853809458789 1105 88-89 -0.7339567539718566 1105 90-91 -3.8734070825483506 1105 92-93 -8.264758834944232 1105 94-95 -5.890459345281936 1105 96-97 -3.4801102431605386 1105 98-99 -2.412110917553532 1105 100-101 -1.7511056796732696 1106 1 1.8517056456480674 1106 2 1.1128384825058255 1106 3 0.8373745320562946 1106 4 0.8416301477043291 1106 5 0.901011204739099 1106 6 0.9413622941784112 1106 7 1.030984672799292 1106 8 -0.3981249271488636 1106 9 -0.38570609740413175 1106 10-11 -0.7195453824023303 1106 12-13 -0.2352343512616457 1106 14-15 0.7201697407212819 1106 16-17 0.6986174951169772 1106 18-19 0.8532149798078024 1106 20-21 0.549049349297988 1106 22-23 0.5341867874772532 1106 24-25 0.6982687685005544 1106 26-27 1.0763307401903859 1106 28-29 0.6821592359034767 1106 30-31 1.0416832668535179 1106 32-33 1.0062693121509696 1106 34-35 0.923876931099521 1106 36-37 0.9265312092494113 1106 38-39 0.833920404197265 1106 40-41 0.6052445257492067 1106 42-43 0.6994783905041899 1106 44-45 1.0018615777914768 1106 46-47 0.7896567207266294 1106 48-49 0.5808715044110428 1106 50-51 0.4599913213471858 1106 52-53 0.7194993374319516 1106 54-55 1.0013924102668064 1106 56-57 0.9029823794245679 1106 58-59 0.919459199470289 1106 60-61 0.4845319947572442 1106 62-63 0.8508002864241284 1106 64-65 0.5673110255157994 1106 66-67 0.7039709009648263 1106 68-69 0.5891022100819434 1106 70-71 0.4779827673013841 1106 72-73 0.6151577490997866 1106 74-75 0.36754939804361797 1106 76-77 0.3458665332121029 1106 78-79 0.5684019169820722 1106 80-81 0.1730911240772457 1106 82-83 0.09584909568667754 1106 84-85 -0.004659300973180791 1106 86-87 0.14589118386563982 1106 88-89 -0.310984791355029 1106 90-91 -4.105005880946216 1106 92-93 -8.344680063248637 1106 94-95 -6.113904605629067 1106 96-97 -3.509724395363474 1106 98-99 -2.699007446258472 1106 100-101 -2.1280215675237386 1107 1 1.7236314424230628 1107 2 0.8172885642679901 1107 3 0.6118233142873706 1107 4 0.731133456093886 1107 5 0.6072806300214779 1107 6 0.7673266545383797 1107 7 0.5127533346071544 1107 8 0.5470280926365021 1107 9 0.4309354810929591 1107 10-11 0.20859190222746804 1107 12-13 0.2166640470662955 1107 14-15 0.35335678656224445 1107 16-17 0.3750592977831815 1107 18-19 0.549380550897574 1107 20-21 0.27254365302005823 1107 22-23 0.12761427364265643 1107 24-25 0.11381752257511835 1107 26-27 0.19700573829523194 1107 28-29 0.0209375142413748 1107 30-31 0.2234287807639106 1107 32-33 -0.031871248380106465 1107 34-35 0.42896473066851826 1107 36-37 0.028113252978634762 1107 38-39 -0.1890034595252459 1107 40-41 -0.22634369075267813 1107 42-43 0.15603141137249565 1107 44-45 -0.06894188437592064 1107 46-47 -0.025171605802214003 1107 48-49 -0.22568246403201186 1107 50-51 -0.22789583062214192 1107 52-53 -0.41025274993986116 1107 54-55 -0.13929942759503433 1107 56-57 -0.008403814140589105 1107 58-59 0.0076432130720469615 1107 60-61 -0.6101645647342089 1107 62-63 -0.7122939783120117 1107 64-65 -0.46694921641356757 1107 66-67 -0.3861745923696631 1107 68-69 -0.3071809956446572 1107 70-71 -0.352734357269501 1107 72-73 -0.5628272467308868 1107 74-75 -0.2638406399676647 1107 76-77 -0.13824534558496282 1107 78-79 -0.21295603885552694 1107 80-81 -0.22693703242961405 1107 82-83 -0.48381080619872563 1107 84-85 0.2354717080044182 1107 86-87 0.6044239591225704 1107 88-89 0.3021637558775083 1107 90-91 -3.3841702012373105 1107 92-93 -4.907586843552078 1107 94-95 -3.528272952300643 1107 96-97 -2.0304668066434033 1107 98-99 -1.5731160491542582 1107 100-101 -1.198058170982657 1108 1 1.6747833943773003 1108 2 0.8714664754217267 1108 3 -0.391525554578223 1108 4 -0.16577499516596106 1108 5 0.2801646781699034 1108 6 0.5268573376106716 1108 7 0.5351668966230179 1108 8 0.5566554965169317 1108 9 0.9039330276639106 1108 10-11 0.6237851014476163 1108 12-13 0.6033053587810642 1108 14-15 0.5825713185428469 1108 16-17 0.6548752098057449 1108 18-19 0.5565540504417683 1108 20-21 0.5479755221223499 1108 22-23 0.32885866882629955 1108 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1.7168881538543808 2311 78-79 1.5334778609978947 2311 80-81 1.9914407383178805 2311 82-83 1.891757102269498 2311 84-85 2.0375203904193313 2311 86-87 2.519785239698834 2311 88-89 2.8497847646065004 2311 90-91 3.1074929866877454 2311 92-93 3.9156791987506665 2311 94-95 3.399809290766992 2311 96-97 2.4819017577626106 2311 98-99 2.7857159922087593 2311 100-101 2.9227876967403006 2312 1 1.2139793749615713 2312 2 0.5079816190910407 2312 3 -0.3452837409899381 2312 4 -0.5833589850928931 2312 5 -0.19051004840462582 2312 6 -0.3752109662929115 2312 7 -0.07382173574543316 2312 8 0.045175442336031324 2312 9 0.25466711338854964 2312 10-11 0.33650609708971047 2312 12-13 0.15636918213171924 2312 14-15 0.366558724482573 2312 16-17 0.002035618506297965 2312 18-19 -0.30498044354737175 2312 20-21 0.13966435535257204 2312 22-23 0.24930500711894865 2312 24-25 0.34975951585360576 2312 26-27 0.4588174666784113 2312 28-29 0.6289138234155089 2312 30-31 0.6297871728366857 2312 32-33 0.7779202731221844 2312 34-35 0.40079770919124513 2312 36-37 0.7051294177134153 2312 38-39 0.7240976172254889 2312 40-41 0.6242407843265312 2312 42-43 0.8576214184173736 2312 44-45 0.787756336694823 2312 46-47 0.7586289162120856 2312 48-49 0.9416315876227657 2312 50-51 0.8352261305501578 2312 52-53 0.6112096022812032 2312 54-55 0.7582581537387192 2312 56-57 0.854792156802155 2312 58-59 0.7493447833495672 2312 60-61 0.7043172898794197 2312 62-63 0.6373781266451992 2312 64-65 0.9651903685157706 2312 66-67 1.199123352295281 2312 68-69 0.9975790163662914 2312 70-71 0.8398373330790072 2312 72-73 0.9317019009922305 2312 74-75 1.3035862068119002 2312 76-77 1.2153591900879377 2312 78-79 1.0728458266911396 2312 80-81 1.1763474037679948 2312 82-83 1.2558182017816257 2312 84-85 1.6602796098226094 2312 86-87 1.8749368882156254 2312 88-89 2.304312349018179 2312 90-91 3.022456704827068 2312 92-93 4.023982028843143 2312 94-95 3.5786370002330123 2312 96-97 2.755707032586969 2312 98-99 1.9960219728119188 2312 100-101 1.9276650844463301 2313 1 -7.091924130270574 2313 2 -2.7736143919868432 2313 3 -1.4989865950371808 2313 4 -0.845925250619338 2313 5 -0.6554853663962348 2313 6 -0.2730058927996808 2313 7 -0.13847588359426055 2313 8 -0.13513450619935696 2313 9 -0.24183373514186002 2313 10-11 -0.042051097176766916 2313 12-13 -0.17652767858215412 2313 14-15 -0.26112506388786016 2313 16-17 -0.6036561687143305 2313 18-19 -0.7744713529397984 2313 20-21 -0.5437778312577777 2313 22-23 -1.2386013615578477 2313 24-25 -0.5634941422245703 2313 26-27 -0.4170953254012346 2313 28-29 -0.85860421131067 2313 30-31 -0.13857024365078274 2313 32-33 -0.4621993226788348 2313 34-35 -0.4135717525585463 2313 36-37 -0.19901013928184597 2313 38-39 -0.21249270013041865 2313 40-41 -0.29469346700430066 2313 42-43 -0.6511492627839459 2313 44-45 -0.6794163157504336 2313 46-47 -1.5151458279850196 2313 48-49 -1.2855893785410473 2313 50-51 -0.8416758766250396 2313 52-53 -1.1157411884828043 2313 54-55 -0.9670888592683937 2313 56-57 -0.7735798533028202 2313 58-59 -0.7870929643687958 2313 60-61 -0.5621406069812949 2313 62-63 -0.9020308594853361 2313 64-65 -1.045065931891635 2313 66-67 -0.8695689158513851 2313 68-69 -0.8312534665940028 2313 70-71 -0.778847748816613 2313 72-73 -0.34133901641563114 2313 74-75 -0.33797741085743027 2313 76-77 -0.17021841865938825 2313 78-79 -0.2117172768848441 2313 80-81 -0.45664591854641756 2313 82-83 -0.011030792972409387 2313 84-85 0.05276154745700978 2313 86-87 0.9198951803201467 2313 88-89 1.2761830130830525 2313 90-91 1.822922843764939 2313 92-93 2.7931451346302865 2313 94-95 2.685292021487733 2313 96-97 1.60690948586252 2313 98-99 1.1029202210250322 2313 100-101 0.9956663669157475 2314 1 0.578986891319424 2314 2 0.03271173984516551 2314 3 -1.5670785719370564 2314 4 -0.5159941709163718 2314 5 -0.19718787616272948 2314 6 -3.5171122717351047 2314 7 -2.8614042491344023 2314 8 -2.347917763397831 2314 9 -3.4254807227982944 2314 10-11 -2.3672722847644394 2314 12-13 -1.116776203635574 2314 14-15 -1.318544621817324 2314 16-17 -2.2313414999045804 2314 18-19 -2.993820969315877 2314 20-21 -2.817771552852278 2314 22-23 -4.441373695375212 2314 24-25 -3.3889768091542862 2314 26-27 -2.0644122789819406 2314 28-29 -2.137568579038387 2314 30-31 -2.0950341145314866 2314 32-33 -2.221885300858183 2314 34-35 -1.4268872525897436 2314 36-37 -1.7103970025016793 2314 38-39 -3.3609281034904086 2314 40-41 -2.59910764447951 2314 42-43 -1.540771978194325 2314 44-45 -2.17295295259661 2314 46-47 -1.5665248867174135 2314 48-49 -2.0774692300526922 2314 50-51 -2.9826745318741352 2314 52-53 -1.9944165870846682 2314 54-55 -2.552955991496429 2314 56-57 -1.483385655025316 2314 58-59 -0.4989097271892611 2314 60-61 -0.9498223550704274 2314 62-63 -1.0594689857120407 2314 64-65 -1.405210750103656 2314 66-67 -0.5859167783937593 2314 68-69 -0.47196271752278207 2314 70-71 -0.5690945330921657 2314 72-73 -1.6546648885816104 2314 74-75 -1.6981965747908383 2314 76-77 -0.8558981271371202 2314 78-79 -1.8885998918813591 2314 80-81 -0.812059084919813 2314 82-83 -0.3134459771974818 2314 84-85 -1.4826988230095353 2314 86-87 -0.292555515773401 2314 88-89 1.170374545069123 2314 90-91 2.188819844839866 2314 92-93 3.4592222336819987 2314 94-95 2.977879426669741 2314 96-97 2.194584488437421 2314 98-99 1.7863561393651413 2314 100-101 1.763122742297087 2315 1 -2.5393150957617934 2315 2 -1.9582060576741434 2315 3 -2.64737775227821 2315 4 -3.997576154626909 2315 5 -3.8053996975296016 2315 6 -3.0997166413043367 2315 7 -5.686009733621166 2315 8 -2.828322439569387 2315 9 -2.449624035075672 2315 10-11 -4.0888171747797735 2315 12-13 -3.3162978262295084 2315 14-15 -2.237491899830431 2315 16-17 -2.60917052211542 2315 18-19 -2.1567431552024416 2315 20-21 -1.5488491498160712 2315 22-23 -1.0338373066060598 2315 24-25 -2.236464841616538 2315 26-27 -3.89919300646563 2315 28-29 -3.532133923252971 2315 30-31 -3.4447475256281592 2315 32-33 -4.01057439767775 2315 34-35 -3.86436395761951 2315 36-37 -3.1207514749739147 2315 38-39 -2.252222023624718 2315 40-41 -1.889954463237352 2315 42-43 -3.1699910011506063 2315 44-45 -6.730977684967787 2315 46-47 -3.2668762153814903 2315 48-49 -2.3972666326837704 2315 50-51 -3.7049389326221664 2315 52-53 -3.4551197953895105 2315 54-55 -1.959674017833116 2315 56-57 -1.3704885061676215 2315 58-59 -2.4812156780615915 2315 60-61 -2.7506762879096094 2315 62-63 -4.222895558345353 2315 64-65 -3.6598065438828016 2315 66-67 -3.67899821274813 2315 68-69 -1.9307901743116211 2315 70-71 -3.303012090774562 2315 72-73 -2.6328814580711146 2315 74-75 -1.6654713954803348 2315 76-77 -3.2532414471929947 2315 78-79 -1.9823311546226954 2315 80-81 -1.583792279320594 2315 82-83 -1.3725827911909967 2315 84-85 -2.732052640791821 2315 86-87 -2.2012234324009086 2315 88-89 -1.576202107131703 2315 90-91 -0.7319493024102748 2315 92-93 -0.339698824322884 2315 94-95 -0.4713095842622863 2315 96-97 -1.0785844863894276 2315 98-99 -1.5630222292901212 2315 100-101 -2.0791168320896283 2316 1 -7.22013688554809 2316 2 -6.017247752896811 2316 3 -3.2417492779647645 2316 4 -3.915111615988046 2316 5 -1.8306605090046162 2316 6 -2.8945011040707733 2316 7 -3.126362305045781 2316 8 -2.4256032160157055 2316 9 -6.932121802515603 2316 10-11 -7.139912513748158 2316 12-13 -5.7772730223140485 2316 14-15 -4.870344800081725 2316 16-17 -4.867796165058106 2316 18-19 -3.956120807449196 2316 20-21 -2.772661566311406 2316 22-23 -3.119526077348098 2316 24-25 -2.9812538041164487 2316 26-27 -3.806607528836224 2316 28-29 -4.064553637623732 2316 30-31 -6.664686709999547 2316 32-33 -4.981431700745183 2316 34-35 -5.900120397430385 2316 36-37 -4.717052694055553 2316 38-39 -6.704954054112143 2316 40-41 -4.073927797124497 2316 42-43 -4.873526926330424 2316 44-45 -3.627699282996211 2316 46-47 -4.757684644420692 2316 48-49 -5.762534228372331 2316 50-51 -5.309889158647241 2316 52-53 -6.736709655067049 2316 54-55 -5.633140474050709 2316 56-57 -4.900054351113731 2316 58-59 -3.7156284282294614 2316 60-61 -3.7950825999972793 2316 62-63 -4.532569812254927 2316 64-65 -3.6244974814195814 2316 66-67 -4.774272281130234 2316 68-69 -3.4443966760803626 2316 70-71 -3.1525885966276768 2316 72-73 -2.894356643771655 2316 74-75 -2.804295961256056 2316 76-77 -3.308550094296205 2316 78-79 -4.961991493971826 2316 80-81 -5.914684507242477 2316 82-83 -3.460114721992017 2316 84-85 -2.2572122082635957 2316 86-87 -2.6268402420206414 2316 88-89 -1.6698450221282783 2316 90-91 -1.4757561115454259 2316 92-93 -1.6839197820046206 2316 94-95 -2.4516639209669577 2316 96-97 -3.7759990433733606 2316 98-99 -4.955598015185879 2316 100-101 -4.254420391032674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8786.0 3 2975.0 4 3045.0 5 3382.0 6 3805.0 7 4652.0 8 5502.0 9 6928.0 10 8168.0 11 9647.0 12 11525.0 13 13266.0 14 14582.0 15 16562.0 16 18364.0 17 19727.0 18 17591.0 19 16212.0 20 17160.0 21 18282.0 22 20496.0 23 22827.0 24 25697.0 25 28379.0 26 31882.0 27 35898.0 28 41127.0 29 46159.0 30 52127.0 31 58651.0 32 66044.0 33 73655.0 34 83987.0 35 95757.0 36 99685.0 37 76940.0 38 21923.0 39 332.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.06701494260082 19.708400702732128 13.810431373190568 21.414152981476484 2 15.788239698876968 20.407864676385433 40.63670582162713 23.167189803110467 3 19.167782965969383 26.271445215301874 29.55163846079745 25.0091333579313 4 12.479152181124942 16.835766904343163 37.03481528946089 33.65026562507101 5 12.994600843180896 38.346009522364106 33.61172783014967 15.04766180430533 6 30.742949383897628 36.63684543951544 17.758490778687595 14.861714397899334 7 28.11219749027623 33.98546389521785 20.49738062810374 17.404957986402177 8 26.816266607275157 34.52531487348245 19.51256539505202 19.145853124190374 9 27.693713350606846 16.48802218282649 19.94699759079958 35.871266875767084 10-11 24.894136363636363 27.134818181818183 26.647136363636363 21.32390909090909 12-13 26.454977397570957 24.802813918333104 27.427931748209033 21.314276935886912 14-15 23.17213963454156 25.719267337731345 26.488146372459987 24.620446655267113 16-17 21.484163373050645 29.25021906940046 27.065388125529495 22.200229432019402 18-19 21.4992342774593 26.739240223230055 30.720497408271257 21.041028091039387 20-21 22.39323528355503 25.824598696134636 31.103612337124797 20.678553683185537 22-23 22.025904643408694 25.969309518742257 30.922906355105166 21.081879482743883 24-25 21.624728166561063 26.188300461633563 31.131541230213188 21.055430141592186 26-27 21.075504099898783 26.65550614700497 31.01748001228264 21.251509740813614 28-29 21.055542894366106 26.78687669987841 31.04130929132669 21.1162711144288 30-31 21.20345703248484 26.56362219716511 30.877999102128555 21.35492166822149 32-33 20.910080422686466 26.760727383876983 30.726551494672073 21.602640698764482 34-35 21.579142457560632 26.816495923774003 30.623156602121682 20.981205016543676 36-37 21.679923185421604 26.37062488259259 30.457835446325543 21.491616485660263 38-39 21.14055150213909 26.664077412043035 30.610901883773696 21.584469202044176 40-41 21.475199652157936 26.521047219287407 30.680143121000192 21.323610007554468 42-43 21.342148071779754 26.596064474260455 30.405390348689092 21.6563971052707 44-45 21.18393690374902 26.90921955951039 30.313002915345834 21.593840621394754 46-47 21.608143049140736 27.100289491218614 29.595690151158532 21.695877308482114 48-49 21.317256673003058 27.323573652136645 29.385100046135243 21.974069628725058 50-51 21.61742464915745 27.531198643038753 29.116701215550457 21.734675492253345 52-53 21.641511253960772 27.414728519922615 29.071564161317387 21.872196064799226 54-55 21.252790211578343 27.542402571226038 29.281410752813414 21.923396464382204 56-57 21.268096445234303 27.762825181072483 28.95822425923239 22.010854114460827 58-59 21.301265366759857 27.26001562944923 29.082056427952153 22.356662575838765 60-61 21.101433906735355 27.679679432057796 29.224995532257104 21.99389112894975 62-63 21.15875431569346 27.527633397158386 29.29315159184464 22.020460695303516 64-65 20.933144782492054 27.458837865980367 29.23982484778763 22.368192503739944 66-67 20.992258454128248 27.550699303879323 29.011743284809217 22.445298957183212 68-69 21.3368505873408 27.332219526125662 29.090876845785296 22.240053040748244 70-71 21.243185893509164 27.389581573955706 28.753789909733413 22.613442622801717 72-73 21.22678460462065 27.551089109451382 28.549488722228645 22.672637563699325 74-75 21.11742045136096 27.84652744669158 28.56322037784466 22.472831724102793 76-77 21.250931036632224 27.53079302708731 28.58301798282752 22.635257953452946 78-79 21.424262139942034 27.62806484341943 28.459764048573415 22.487908968065113 80-81 21.49059979650639 27.825248497004928 28.124436888768358 22.559714817720327 82-83 21.57000683921386 27.611585437161366 28.105528660310398 22.712879063314375 84-85 21.465868076058385 27.50724909312412 28.128482953826893 22.8983998769906 86-87 21.46658453565193 27.516179779574674 28.17788340368152 22.83935228109188 88-89 21.554681258769 27.536163313139987 27.92682172291869 22.982333705172323 90-91 21.604881424672822 27.349965214743115 28.143369966509503 22.901783394074563 92-93 21.886886523550125 26.905325116893426 28.271109643998404 22.936678715558045 94-95 21.507734681832424 26.830201327610958 28.347444968267038 23.31461902228958 96-97 21.924874744973074 26.73906404947488 27.854060201600404 23.482001003951638 98-99 21.918797887891063 26.810845703968784 27.64902463595984 23.621331772180305 100-101 22.119258368848577 26.818835828506906 27.66833315120388 23.393572651440646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2665.0 1 1737.5 2 939.5 3 1085.5 4 973.0 5 866.5 6 1162.5 7 2146.0 8 3286.0 9 3669.5 10 3141.0 11 2443.0 12 2185.0 13 2154.5 14 2186.5 15 2245.5 16 2378.5 17 2506.5 18 2647.0 19 2861.0 20 3166.0 21 3551.0 22 4012.5 23 4713.0 24 5415.5 25 6292.5 26 7532.5 27 8999.5 28 10528.0 29 12140.0 30 13955.5 31 15991.0 32 18719.5 33 20715.5 34 21899.0 35 23684.0 36 26163.5 37 28435.0 38 29776.0 39 30736.5 40 31697.0 41 32862.0 42 33754.5 43 34846.0 44 36177.0 45 37190.5 46 37486.5 47 37988.5 48 38430.5 49 37833.5 50 37216.5 51 36194.0 52 34865.5 53 34457.5 54 34341.0 55 32532.5 56 29735.0 57 27265.5 58 25043.5 59 22253.5 60 18760.0 61 15792.0 62 13171.0 63 10340.5 64 7984.5 65 6343.0 66 4843.5 67 3579.5 68 2671.0 69 1975.0 70 1470.0 71 1063.0 72 794.5 73 635.0 74 501.5 75 366.0 76 260.0 77 186.0 78 126.0 79 94.0 80 64.0 81 42.5 82 41.0 83 26.5 84 12.5 85 9.5 86 6.5 87 6.5 88 7.0 89 4.0 90 2.0 91 1.5 92 0.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.131611551682041 2 0.1191765292127723 3 0.1238963917558524 4 0.13542374835145188 5 0.12435022469268703 6 0.13651294739985495 7 0.16746435369197632 8 0.15266939995116757 9 0.16129222575102545 10-11 0.1567538963826792 12-13 0.14740493788388595 14-15 0.15285093312590142 16-17 0.14695110494705133 18-19 0.1620637417436443 20-21 0.16732820381092595 22-23 0.20268178959034316 24-25 0.2249649867889232 26-27 0.23730924267082498 28-29 0.23304321306457954 30-31 0.2233311882163186 32-33 0.2244203872647217 34-35 0.21461759582909376 36-37 0.22296812186685086 38-39 0.20980696669864676 40-41 0.21579756146486379 42-43 0.2069932024902721 44-45 0.19973187550091812 46-47 0.2164329275764323 48-49 0.25269417922951876 50-51 0.2542372112147565 52-53 0.25641560931156265 54-55 0.25378337827792186 56-57 0.22805105075939866 58-59 0.22873180016465058 60-61 0.19773501057884577 62-63 0.19119981628842717 64-65 0.19169903251894527 66-67 0.20014032514406926 68-69 0.19923265927040001 70-71 0.2052686373303005 72-73 0.23762692572660923 74-75 0.24062222310971773 76-77 0.25573485990631073 78-79 0.25160498018111566 80-81 0.26512920169878745 82-83 0.2647207520556363 84-85 0.23817152525081076 86-87 0.24234678826968933 88-89 0.2437082870801932 90-91 0.4536514036598904 92-93 0.599558692852222 94-95 0.34627453080481824 96-97 0.2785172733354089 98-99 0.3080164142296594 100-101 0.3273950806324979 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1101727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.30418154552335 #Duplication Level Percentage of deduplicated Percentage of total 1 84.12569760280594 54.937598288974385 2 9.533597693622548 12.451675891326193 3 2.775238533418452 5.437040430554717 4 1.144054508298322 2.9884617323155234 5 0.6163320690397385 2.012453066444956 6 0.3926280771906552 1.538415313963698 7 0.24905857094349512 1.1385196292653827 8 0.17774072848129263 0.9285770240620725 9 0.1384892026650087 0.8139531629637455 >10 0.7675983867108744 9.310013159553042 >50 0.0511213998022279 2.310067580068613 >100 0.025471548598232342 3.065735136606652 >500 0.001839610452419755 0.7951963568556943 >1k 8.490509780398869E-4 1.031676017191978 >5k 2.830169926799623E-4 1.240617209853306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7640 0.6934567274833058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5785 0.5250847079176603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4133 0.37513830558750033 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2271 0.2061309199102863 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1424 0.12925162041050098 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1181 0.10719533968033823 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1110 0.10075091197728657 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.076658736692484E-5 0.0 0.0 0.0 0.0 6 1.8153317473384967E-4 0.0 0.0 0.0 0.0 7 1.8153317473384967E-4 0.0 0.0 9.076658736692484E-5 0.0 8 1.8153317473384967E-4 0.0 0.0 9.076658736692484E-5 0.0 9 1.8153317473384967E-4 0.0 0.0 1.8153317473384967E-4 0.0 10-11 1.8153317473384967E-4 0.0 0.0 4.0844964315116174E-4 0.0 12-13 3.6306634946769935E-4 0.0 0.0 8.168992863023235E-4 0.0 14-15 8.168992863023235E-4 0.0 0.0 8.62282579985786E-4 0.0 16-17 8.168992863023235E-4 0.0 0.0 9.076658736692484E-4 0.0 18-19 9.076658736692484E-4 0.0 0.0 0.0013614988105038724 0.0 20-21 9.984324610361733E-4 0.0 0.0 0.001724565159971572 0.0 22-23 9.984324610361733E-4 0.0 0.0 0.0023599312715400457 0.0 24-25 0.0012707322231369476 0.0 0.0 0.003539896907310069 0.0 26-27 0.0012707322231369476 0.0 0.0 0.00599059476621704 0.0 28-29 0.0013161155168204101 0.0 0.0 0.013841904573456038 0.0 30-31 0.0013614988105038726 0.0 0.0 0.03762275046359034 0.0 32-33 0.0013614988105038726 0.0 0.0 0.08309681073441968 0.0 34-35 0.001406882104187335 0.0 0.0 0.14885720328175672 0.0 36-37 0.0014522653978707974 0.0 0.0 0.2580947911778508 0.0 38-39 0.001633798572604647 0.0 0.0 0.41829781788047316 0.0 40-41 0.0016791818662881095 0.0 0.0 0.5960187959449119 0.0 42-43 0.0019060983347054215 0.0 0.0 0.7653892479715937 0.0 44-45 0.0019968649220723465 0.0 0.0 0.9692510031977069 0.0 46-47 0.002178398096806196 0.0 0.0 1.2140031060326197 0.0 48-49 0.002178398096806196 0.0 0.0 1.4773169759840687 0.0 50-51 0.002178398096806196 0.0 0.0 1.759510296107838 0.0 52-53 0.002178398096806196 0.0 0.0 2.0269086624907984 0.0 54-55 0.002178398096806196 0.0 0.0 2.2955323778032124 0.0 56-57 0.0023599312715400457 0.0 0.0 2.6159384312084573 0.0 58-59 0.0023599312715400457 0.0 0.0 2.9669328245563555 0.0 60-61 0.0023599312715400457 0.0 0.0 3.3521916046352684 0.0 62-63 0.0024506978589069707 0.0 0.0 3.7118995903703915 0.0 64-65 0.0026322310336408202 0.0 0.0 4.068884578484507 0.0 66-67 0.0026322310336408202 0.0 0.0 4.466442231151637 0.0 68-69 0.0026322310336408202 0.0 0.0 4.915283005681081 0.0 70-71 0.002722997621007745 0.0 0.0 5.397979717298387 0.0 72-73 0.00281376420837467 0.0 0.0 5.8650191925948985 0.0 74-75 0.00281376420837467 0.0 0.0 6.2985204138593325 0.0 76-77 0.00281376420837467 0.0 0.0 6.758343945460172 0.0 78-79 0.00281376420837467 0.0 0.0 7.279934139764206 0.0 80-81 0.002904530795741595 0.0 0.0 7.833020339884563 0.0 82-83 0.002904530795741595 0.0 0.0 8.347530740373976 0.0 84-85 0.0033129804388927564 0.0 9.076658736692484E-5 8.824055324050331 0.0 86-87 0.0036306634946769935 0.0 9.076658736692484E-5 9.291412482402627 0.0 88-89 0.0036306634946769935 0.0 9.076658736692484E-5 9.801339170230012 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2845 0.0 57.357944 1 GTATCAA 5735 0.0 43.92164 1 TATCAAC 7465 0.0 34.696068 2 TGGTATC 1165 0.0 34.610703 2 ATCAACG 7640 0.0 33.90133 3 TCAACGC 7765 0.0 33.35559 4 CAACGCA 7910 0.0 32.6242 5 AACGCAG 8080 0.0 32.172634 6 GTGGTAT 1370 0.0 30.470499 1 ACGCAGA 9120 0.0 28.035696 7 GTACATG 6570 0.0 27.870152 1 TACATGG 6715 0.0 27.833488 2 CGCAGAG 9245 0.0 27.500196 8 ACATGGG 6990 0.0 26.195553 3 GCAGAGT 9380 0.0 25.486229 9 TAAGGTG 475 0.0 23.96818 5 GAGTACT 5785 0.0 23.53187 12-13 GTATAGG 270 4.1836756E-11 22.84005 1 AGTACTT 6000 0.0 22.530535 12-13 CATGGGG 2525 0.0 22.356457 4 >>END_MODULE