FastQCFastQC Report
Fri 27 May 2016
SRR939885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101727
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82590.7496412450634322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71370.6478011340377425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49590.45011150675258027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29320.26612763415982366No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.15965842717842077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15210.13805597938509268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.056.9548871
GTATCAA59700.041.222421
TGGTATC14750.030.3495182
TATCAAC80600.030.284122
ATCAACG81000.030.188633
TCAACGC82000.029.7055594
CAACGCA82700.029.5110935
GTGGTAT15800.028.645171
AACGCAG85350.028.5740496
GTACATG66500.026.4438381
TACATGG66050.026.1826422
ACATGGG66600.025.0432173
ACGCAGA98700.024.4706677
CGCAGAG98850.024.433538
GCAGAGT102700.022.9216129
GAGTACT64400.021.82297112-13
CATGGGA40650.021.6741524
AGTACTT65850.021.30668612-13
CATGGGG25050.020.8773964
AGAGTAC98800.020.56219310-11
Produced by FastQC (version 0.11.5)