##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939885_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1101727 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35313466947801 31.0 31.0 34.0 30.0 34.0 2 31.55608512816696 31.0 31.0 34.0 30.0 34.0 3 31.591029356637353 31.0 31.0 34.0 30.0 34.0 4 35.430412434296336 37.0 35.0 37.0 33.0 37.0 5 35.17042969810125 37.0 35.0 37.0 32.0 37.0 6 35.20664828945828 37.0 35.0 37.0 32.0 37.0 7 35.12206109135929 37.0 35.0 37.0 32.0 37.0 8 35.060841751177925 37.0 35.0 37.0 32.0 37.0 9 36.64050803874281 39.0 35.0 39.0 32.0 39.0 10-11 36.4926987357122 38.0 35.0 39.0 32.0 39.0 12-13 36.429589181348916 38.0 35.0 39.0 32.0 39.0 14-15 37.39524401235515 39.0 36.0 41.0 32.0 41.0 16-17 37.33466639194646 39.0 36.0 41.0 32.0 41.0 18-19 37.35682206208979 39.0 36.0 40.5 32.0 41.0 20-21 37.307486791192375 39.0 36.0 40.0 31.5 41.0 22-23 37.15594698142098 39.0 36.0 40.0 31.0 41.0 24-25 36.985130617657546 39.0 36.0 40.0 31.0 41.0 26-27 36.656222911846584 38.5 35.0 40.0 30.0 41.0 28-29 36.414915854835186 38.0 35.0 40.0 30.0 41.0 30-31 36.09983326177901 38.0 35.0 40.0 30.0 41.0 32-33 35.94789498668908 38.0 34.5 40.0 29.5 41.0 34-35 35.94650444257062 38.0 35.0 40.0 30.0 41.0 36-37 35.85438407155311 38.0 35.0 40.0 29.0 41.0 38-39 35.67948774968754 38.0 34.5 40.0 28.5 41.0 40-41 35.44875999226669 38.0 34.0 40.0 27.0 41.0 42-43 35.165221965151076 38.0 34.0 40.0 26.5 41.0 44-45 34.82819246510252 38.0 33.5 40.0 25.5 41.0 46-47 34.40602889826609 38.0 33.0 40.0 24.0 41.0 48-49 33.998875855815456 38.0 33.0 40.0 23.0 41.0 50-51 33.66805342884399 37.0 33.0 40.0 22.5 41.0 52-53 33.35300441942513 37.0 32.5 40.0 20.5 41.0 54-55 33.01623995781169 37.0 32.0 40.0 19.5 41.0 56-57 31.755824264994867 35.5 30.0 39.5 12.0 40.5 58-59 28.459609776287593 34.0 22.5 38.0 2.0 40.0 60-61 28.47523524430281 33.5 23.0 38.0 2.0 40.0 62-63 30.172741069248552 34.5 28.5 38.0 2.0 40.0 64-65 29.61934717039702 34.0 28.0 38.0 2.0 40.0 66-67 24.210283491282322 28.0 16.5 35.0 2.0 38.5 68-69 16.67032123202935 13.0 7.0 28.5 2.0 34.5 70-71 15.597521436798772 12.0 7.0 25.0 2.0 33.0 72-73 17.580803592904594 16.0 7.0 29.0 2.0 34.0 74-75 18.321111309789085 17.5 7.5 30.0 2.0 34.5 76-77 19.422153582511818 21.5 7.5 30.5 2.0 34.5 78-79 22.506521125469376 27.5 9.5 33.5 2.0 35.5 80-81 25.028001492202698 31.0 17.5 34.5 2.0 36.0 82-83 25.893522624025735 32.0 22.5 35.0 2.0 36.0 84-85 25.857848632192912 33.0 22.5 35.0 2.0 36.0 86-87 25.55576381444768 32.5 20.5 35.0 2.0 35.0 88-89 25.101499282490128 32.0 18.5 35.0 2.0 35.0 90-91 23.55491242385818 31.5 4.5 34.0 2.0 35.0 92-93 21.524307292096864 30.0 2.0 34.0 2.0 35.0 94-95 17.14316886125147 18.5 2.0 33.0 2.0 35.0 96-97 15.831342519517086 10.0 2.0 31.5 2.0 34.5 98-99 14.716736995644112 2.0 2.0 31.0 2.0 34.0 100-101 14.099667158924126 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3750121993797073 1101 2 0.5331711200977018 1101 3 0.5393507341218999 1101 4 0.32271670891817905 1101 5 0.43964019573790125 1101 6 0.47852582943677646 1101 7 0.6668316593096364 1101 8 0.42324721902819107 1101 9 0.3998818229206904 1101 10-11 0.3991239562280313 1101 12-13 0.41726262035592043 1101 14-15 0.4803563165310365 1101 16-17 0.4321012078121669 1101 18-19 0.5284037561686787 1101 20-21 0.701414548086781 1101 22-23 0.680105537313672 1101 24-25 0.6800448717523366 1101 26-27 0.964455005737463 1101 28-29 0.5799037213463905 1101 30-31 0.4729772544809663 1101 32-33 0.5449087022737373 1101 34-35 0.8649004650219823 1101 36-37 0.8413462388747845 1101 38-39 0.6819202834968436 1101 40-41 0.8217393457780346 1101 42-43 0.7840167993849718 1101 44-45 0.8235469469104686 1101 46-47 0.6550634387443068 1101 48-49 0.6665400904284766 1101 50-51 0.5829132803933064 1101 52-53 0.7527868849922115 1101 54-55 0.6196254184765522 1101 56-57 1.1755256970074868 1101 58-59 -8.098306730459758 1101 60-61 -9.094444869320295 1101 62-63 -2.8395389201140695 1101 64-65 -0.34928528772958956 1101 66-67 -1.5689464393208965 1101 68-69 -4.202548126403592 1101 70-71 -4.381364646103995 1101 72-73 -6.849571528674268 1101 74-75 -8.010809021762896 1101 76-77 -8.622838842017641 1101 78-79 -11.97205742029297 1101 80-81 -8.305290867206399 1101 82-83 -5.066437255824354 1101 84-85 -4.118172850387044 1101 86-87 -4.043872313286943 1101 88-89 -3.887473198788033 1101 90-91 -8.807823438817781 1101 92-93 -13.349848145704083 1101 94-95 -11.605286811707794 1101 96-97 -11.659196247181203 1101 98-99 -11.164541873137694 1101 100-101 -10.795259416214499 1102 1 0.5768218838935866 1102 2 0.6109050803585809 1102 3 0.5617608660800393 1102 4 0.40619539843736874 1102 5 0.41592659798270404 1102 6 0.5011966545543274 1102 7 0.667405184260339 1102 8 0.6712896503947903 1102 9 0.5687304123337071 1102 10-11 0.6102877852891524 1102 12-13 0.7519096892168378 1102 14-15 0.9149578825859379 1102 16-17 0.9470555154111366 1102 18-19 0.8620673008372606 1102 20-21 0.5051860671221178 1102 22-23 0.8050984275002335 1102 24-25 0.9996475753773879 1102 26-27 0.9339657557753753 1102 28-29 0.8927212930133024 1102 30-31 1.0065592732887936 1102 32-33 0.953234767797781 1102 34-35 0.8766648363248848 1102 36-37 1.0988210227364519 1102 38-39 0.9883082138775521 1102 40-41 1.0094360506165003 1102 42-43 1.2009172999158437 1102 44-45 1.0866337435129196 1102 46-47 0.9030892805462116 1102 48-49 0.5893583256779777 1102 50-51 0.7627228321906685 1102 52-53 0.6516142397624165 1102 54-55 0.5635574297005803 1102 56-57 1.2529610451039588 1102 58-59 -8.819090137103167 1102 60-61 -9.52415488372952 1102 62-63 -3.1064579061598714 1102 64-65 -0.7465153044139505 1102 66-67 -1.1048912861281153 1102 68-69 -3.7444746962504 1102 70-71 -4.07420885587193 1102 72-73 -6.698585160556567 1102 74-75 -7.76790935545014 1102 76-77 -8.634242982772989 1102 78-79 -11.750868754283568 1102 80-81 -8.152714270816286 1102 82-83 -4.198570768640977 1102 84-85 -3.2233293610802 1102 86-87 -2.9961465056121384 1102 88-89 -3.3793348323387704 1102 90-91 -8.342701813609226 1102 92-93 -13.49828683067299 1102 94-95 -11.810691691883738 1102 96-97 -11.474284219272818 1102 98-99 -10.788212338024696 1102 100-101 -10.665604858350097 1103 1 0.2664191341432627 1103 2 0.2241014594273878 1103 3 0.12181452643377 1103 4 0.10856440706918846 1103 5 0.2092713886090536 1103 6 0.29532875318150786 1103 7 0.30212398161022946 1103 8 0.16676348640162075 1103 9 0.31185775054704834 1103 10-11 0.3368685146403152 1103 12-13 0.3366789931177223 1103 14-15 0.37432296267652987 1103 16-17 0.477810805458148 1103 18-19 0.37036945799263066 1103 20-21 0.30279020545979307 1103 22-23 0.3976979608288431 1103 24-25 0.49172250333128176 1103 26-27 0.3525405111842588 1103 28-29 0.4849328857522437 1103 30-31 0.45409473642929044 1103 32-33 0.48494819682693446 1103 34-35 0.4147538604057672 1103 36-37 0.28278656401356983 1103 38-39 0.23882413486794007 1103 40-41 0.3782584252471253 1103 42-43 0.4632239866632375 1103 44-45 0.26639592850401783 1103 46-47 0.07054992085958389 1103 48-49 0.10239861436519959 1103 50-51 -0.03791227054788493 1103 52-53 -0.06107090331281029 1103 54-55 -0.04584867267917048 1103 56-57 0.5731411546643201 1103 58-59 -6.022380147547281 1103 60-61 -8.744734817773903 1103 62-63 -2.659964569139486 1103 64-65 -0.5205179142999796 1103 66-67 -1.8735455722145922 1103 68-69 -4.316866467110632 1103 70-71 -4.755449671564156 1103 72-73 -7.681996843795934 1103 74-75 -8.086383440561676 1103 76-77 -8.878580421382388 1103 78-79 -11.763499962761355 1103 80-81 -8.2042823735297 1103 82-83 -4.494641427534361 1103 84-85 -3.7719179200894466 1103 86-87 -3.5320878585935063 1103 88-89 -3.6055710430219357 1103 90-91 -8.855370159256864 1103 92-93 -14.325372739802335 1103 94-95 -12.08056293445916 1103 96-97 -11.945889139205079 1103 98-99 -11.462572493155225 1103 100-101 -11.018664504072923 1104 1 0.17747930464286554 1104 2 0.150564212202962 1104 3 0.10711389201663835 1104 4 0.25910651154975994 1104 5 0.1696977615273738 1104 6 0.2518810925946724 1104 7 0.2417905408885801 1104 8 0.24598570587029656 1104 9 0.1515676781838451 1104 10-11 0.23084681807013396 1104 12-13 0.1409879493032875 1104 14-15 0.18975187574155683 1104 16-17 0.23907078018059025 1104 18-19 0.41551313116868016 1104 20-21 0.3847204691394097 1104 22-23 0.22375274126104472 1104 24-25 0.30812875333128176 1104 26-27 0.4336573083690354 1104 28-29 0.4792869450305943 1104 30-31 0.4203662347441224 1104 32-33 0.45656577464216497 1104 34-35 0.4118165834430343 1104 36-37 0.39993998887478455 1104 38-39 0.47610202691789993 1104 40-41 0.5635156615675072 1104 42-43 0.3587084112270844 1104 44-45 -0.01666686887901392 1104 46-47 0.19149435979693408 1104 48-49 0.0730162417442628 1104 50-51 -0.031663199869854 1104 52-53 -0.02718844395516129 1104 54-55 -0.035164877576079334 1104 56-57 0.605398229902228 1104 58-59 -6.524972026512387 1104 60-61 -8.404601119320294 1104 62-63 -2.610591551693016 1104 64-65 -0.41665782062432655 1104 66-67 -1.8865656827419528 1104 68-69 -4.126314573772014 1104 70-71 -4.84873717899873 1104 72-73 -7.459789456305847 1104 74-75 -8.327009679657632 1104 76-77 -8.908426835438695 1104 78-79 -11.97156399924034 1104 80-81 -8.227021952732713 1104 82-83 -4.977128045298038 1104 84-85 -4.100409692492303 1104 86-87 -3.9035762606553632 1104 88-89 -3.8910299422090873 1104 90-91 -8.936585774344096 1104 92-93 -14.20266475754619 1104 94-95 -11.91741674591832 1104 96-97 -11.5352858853391 1104 98-99 -10.977350261295589 1104 100-101 -10.601920600425025 1105 1 0.18710430464286532 1105 2 0.15819367648867555 1105 3 0.05289960630235413 1105 4 0.1947091901211877 1105 5 -0.0552977741869114 1105 6 0.08880966402324475 1105 7 0.11713429088857907 1105 8 0.1477490987274379 1105 9 0.2640453567552754 1105 10-11 0.17414369307013544 1105 12-13 0.24116205644615007 1105 14-15 0.14824071502727065 1105 16-17 0.3094301551805927 1105 18-19 0.19986804188296503 1105 20-21 0.2501222548537001 1105 22-23 0.24400497340389649 1105 24-25 0.2157225033312855 1105 26-27 0.22296534408332036 1105 28-29 0.10337176645916912 1105 30-31 0.02778364545840617 1105 32-33 0.2728090782135908 1105 34-35 0.4297764048716104 1105 36-37 0.2877390960176385 1105 38-39 0.526206937632189 1105 40-41 0.6034978044246486 1105 42-43 0.47462582194137326 1105 44-45 0.3968420596924176 1105 46-47 0.4696640026540848 1105 48-49 0.32737561674426274 1105 50-51 0.3144461751301506 1105 52-53 0.32433387747341413 1105 54-55 0.1805784259953498 1105 56-57 0.8386102834736562 1105 58-59 -6.7186751515123895 1105 60-61 -8.345141297891725 1105 62-63 -2.7514665516930137 1105 64-65 -0.6331913027671803 1105 66-67 -1.9702688077419523 1105 68-69 -4.116287788057727 1105 70-71 -4.621645661141587 1105 72-73 -6.157439009877276 1105 74-75 -6.612000751086203 1105 76-77 -8.194429067581552 1105 78-79 -11.311242570668911 1105 80-81 -8.177475077732716 1105 82-83 -4.804145902440897 1105 84-85 -3.9117846924923043 1105 86-87 -3.757201260655364 1105 88-89 -3.8650277100662294 1105 90-91 -8.593775506486953 1105 92-93 -11.153707168260475 1105 94-95 -11.118564067346892 1105 96-97 -11.440587224624814 1105 98-99 -10.909164993438447 1105 100-101 -10.410233100425025 1106 1 0.13142206165220927 1106 2 0.17440328930576499 1106 3 0.05232417239047038 1106 4 0.1035209391198535 1106 5 0.12534174517223562 1106 6 0.013680158015233701 1106 7 -0.054931129672162626 1106 8 0.053396628767487186 1106 9 0.03888514313712221 1106 10-11 0.2595595809206017 1106 12-13 0.12220144229394236 1106 14-15 0.221202664292953 1106 16-17 0.07974323929273908 1106 18-19 0.0923640365425129 1106 20-21 0.17901077287772438 1106 22-23 0.1070069760741248 1106 24-25 0.318825307069595 1106 26-27 0.05848937612604743 1106 28-29 0.08874226045115563 1106 30-31 0.2282188924544002 1106 32-33 0.4191862477730055 1106 34-35 0.3503898895178068 1106 36-37 0.4181569865383352 1106 38-39 0.3863057360300459 1106 40-41 0.27598645596003024 1106 42-43 0.3210003212738144 1106 44-45 0.3738267058873461 1106 46-47 0.3928816261520751 1106 48-49 0.18471673823958668 1106 50-51 0.252175147092764 1106 52-53 0.0725268013719429 1106 54-55 -0.3642500119218752 1106 56-57 0.23084726611718054 1106 58-59 -7.403151787026406 1106 60-61 -9.827943701095997 1106 62-63 -3.115293654496753 1106 64-65 -0.33422134282058735 1106 66-67 -2.2946519853120435 1106 68-69 -4.934740391529022 1106 70-71 -5.301026168484713 1106 72-73 -6.697694684109585 1106 74-75 -7.2959787217137055 1106 76-77 -8.959963780532153 1106 78-79 -11.995337363726321 1106 80-81 -8.621264797358883 1106 82-83 -5.065591830344765 1106 84-85 -4.046350113053052 1106 86-87 -3.7277292980385397 1106 88-89 -3.7809649597324544 1106 90-91 -8.780193397007649 1106 92-93 -12.156399424602265 1106 94-95 -11.75004671087159 1106 96-97 -12.021745435572743 1106 98-99 -11.311115594239514 1106 100-101 -10.790924689210073 1107 1 0.005792601282493592 1107 2 0.11156960762590629 1107 3 0.011068121448854384 1107 4 -0.1406988549044712 1107 5 -0.06496239924899072 1107 6 0.0037495746340709957 1107 7 0.15186893747027597 1107 8 0.02686182840795226 1107 9 -0.167121668571653 1107 10-11 0.1225423025718726 1107 12-13 -0.0018069884139748638 1107 14-15 -0.06021417654697103 1107 16-17 -0.011167759072009176 1107 18-19 0.12968562175384335 1107 20-21 0.2211866481660607 1107 22-23 0.2065747466202552 1107 24-25 0.06356491352826765 1107 26-27 0.2587342565150408 1107 28-29 0.3410509586458943 1107 30-31 0.14243088929220704 1107 32-33 0.22997113747530307 1107 34-35 0.22245443830978218 1107 36-37 0.2587491936835917 1107 38-39 -0.03463133417712072 1107 40-41 0.015481913015946702 1107 42-43 -0.021443040829694837 1107 44-45 0.10834942602249953 1107 46-47 0.05320315180156854 1107 48-49 0.139116057068712 1107 50-51 0.14620515543142432 1107 52-53 0.0182679943414783 1107 54-55 -0.00832672215893382 1107 56-57 0.748017997428299 1107 58-59 -6.316677469009488 1107 60-61 -8.558433136991209 1107 62-63 -2.6956473164670562 1107 64-65 -0.5108201721596686 1107 66-67 -1.9158725157373162 1107 68-69 -4.567995121280704 1107 70-71 -4.700507438992938 1107 72-73 -6.092214047122765 1107 74-75 -7.054296397502359 1107 76-77 -9.342936268376121 1107 78-79 -12.631637869460501 1107 80-81 -8.974731161162609 1107 82-83 -5.154854725483439 1107 84-85 -4.312391876733326 1107 86-87 -4.011379707932306 1107 88-89 -3.7186294316355095 1107 90-91 -8.718699856760086 1107 92-93 -11.965267505952909 1107 94-95 -11.412739203913684 1107 96-97 -12.061411591451497 1107 98-99 -11.439892356457815 1107 100-101 -10.848420817690378 1108 1 -0.08290061742440358 1108 2 -0.16274010998735733 1108 3 -0.12548454932872488 1108 4 -0.15166371507041987 1108 5 -0.30186896837418686 1108 6 -0.12038100748736014 1108 7 -0.07016021280296769 1108 8 -0.1276100949910699 1108 9 0.12003504385242536 1108 10-11 -0.062456799136590746 1108 12-13 -0.1217921214514277 1108 14-15 -0.08990389999675585 1108 16-17 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58-59 4.506223071427836 2311 60-61 4.427187804346904 2311 62-63 1.9368791672084384 2311 64-65 1.6588400842625894 2311 66-67 -0.2574707463526167 2311 68-69 -3.0290230753875225 2311 70-71 -0.5510714631263571 2311 72-73 -1.0362730740360568 2311 74-75 1.4123001951404284 2311 76-77 4.405544161128351 2311 78-79 5.1857562753961695 2311 80-81 3.1585249222672864 2311 82-83 1.9335494587407354 2311 84-85 1.5270489100925104 2311 86-87 1.498165459861596 2311 88-89 1.7269861371527853 2311 90-91 3.1304723587334458 2311 92-93 5.075910186920694 2311 94-95 8.771914584857125 2311 96-97 9.372486857996927 2311 98-99 9.808048946095363 2311 100-101 8.928897755794685 2312 1 -0.2871282534966717 2312 2 -0.3262448617173028 2312 3 -0.30555222094017154 2312 4 -0.2486462916063843 2312 5 -0.3306266778413942 2312 6 -0.18570196388373006 2312 7 -0.16907168917786208 2312 8 -0.07344691456160035 2312 9 -0.2250908558693112 2312 10-11 -0.31294600792654137 2312 12-13 -0.4079608671418882 2312 14-15 -0.5577758962684101 2312 16-17 -0.3579352933243882 2312 18-19 -0.3230289680838112 2312 20-21 -0.3912398713921519 2312 22-23 -0.2713637973602232 2312 24-25 -0.24457317773184428 2312 26-27 -0.3480313336575449 2312 28-29 -0.36707673852422573 2312 30-31 -0.2157412714851077 2312 32-33 -0.34770254969338765 2312 34-35 -0.4421571499456647 2312 36-37 -0.3408589106268778 2312 38-39 -0.37965352748409487 2312 40-41 -0.31143242813348593 2312 42-43 -0.40983264981943535 2312 44-45 -0.3856297011049179 2312 46-47 -0.19683101395721536 2312 48-49 -0.07631541315606682 2312 50-51 -0.15003555244460642 2312 52-53 -0.18117775043356232 2312 54-55 -0.28631858397142906 2312 56-57 0.9926866954337896 2312 58-59 4.204740130879639 2312 60-61 3.8764334529388407 2312 62-63 1.454576637675757 2312 64-65 1.28237015902684 2312 66-67 -0.18417910674527604 2312 68-69 -0.9744406119779931 2312 70-71 0.022560318924858436 2312 72-73 6.6720094951061135 2312 74-75 4.9671919399436995 2312 76-77 5.310049337734064 2312 78-79 5.684561416042051 2312 80-81 3.222478410639379 2312 82-83 1.8872095792866794 2312 84-85 1.6864777659794576 2312 86-87 1.475400068248291 2312 88-89 1.6420719577078557 2312 90-91 3.0909487460047416 2312 92-93 5.061505456324241 2312 94-95 8.918621979164737 2312 96-97 9.355841346803755 2312 98-99 9.989044308887133 2312 100-101 9.011404773329128 2313 1 -0.3702068434436576 2313 2 -0.21102714595011562 2313 3 -0.14199176522129875 2313 4 -0.396424634456892 2313 5 -0.3724520408852712 2313 6 -0.3279233999178075 2313 7 -0.41955123323787546 2313 8 -0.37377692932105333 2313 9 -0.27015003188270725 2313 10-11 -0.503999401771793 2313 12-13 -0.5068892865536156 2313 14-15 -0.5655225366960437 2313 16-17 -0.4199408930723223 2313 18-19 -0.551307142333819 2313 20-21 -0.5240279709604252 2313 22-23 -0.4932144228404596 2313 24-25 -0.5602741688816977 2313 26-27 -0.647807177903843 2313 28-29 -0.725095074520155 2313 30-31 -0.6900870462458926 2313 32-33 -0.49016168480043376 2313 34-35 -0.4337520001676083 2313 36-37 -0.849949518196766 2313 38-39 -0.982658762391587 2313 40-41 -1.2196322169682645 2313 42-43 -0.6926558514601027 2313 44-45 -0.5416694687126196 2313 46-47 -0.7027106110849246 2313 48-49 -0.5377425196950014 2313 50-51 -0.3122326934222599 2313 52-53 -0.37352397848095364 2313 54-55 -0.4889846831085265 2313 56-57 0.8191104023231901 2313 58-59 4.147128509053335 2313 60-61 3.925183874440105 2313 62-63 1.6051790389891814 2313 64-65 1.36835088881827 2313 66-67 2.0262170491632077 2313 68-69 0.6042308713195013 2313 70-71 2.4729447833556772 2313 72-73 7.955598308877182 2313 74-75 9.608449450958009 2313 76-77 8.258800312023869 2313 78-79 6.013450559827881 2313 80-81 3.3519525428995642 2313 82-83 1.880780648545553 2313 84-85 1.5525281194877287 2313 86-87 1.120565794253121 2313 88-89 1.2842553895296795 2313 90-91 2.913037899740761 2313 92-93 4.952895636588586 2313 94-95 8.452811259489337 2313 96-97 9.102483847398007 2313 98-99 9.620250742240183 2313 100-101 8.68824942869311 2314 1 -0.3298686683301071 2314 2 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4.037543469825302 2314 62-63 1.4183398999198893 2314 64-65 1.2779602727714874 2314 66-67 2.098550721464676 2314 68-69 0.0380258250945964 2314 70-71 2.4903638045452983 2314 72-73 8.297468948772618 2314 74-75 9.766283966811418 2314 76-77 8.695718148759216 2314 78-79 6.4818634201145 2314 80-81 3.7352468054407844 2314 82-83 2.0458966931500875 2314 84-85 1.4785762490944414 2314 86-87 1.6335886260054906 2314 88-89 1.7796289096871618 2314 90-91 3.141348170185111 2314 92-93 5.1437664897293125 2314 94-95 8.703689264325796 2314 96-97 8.981370677978253 2314 98-99 9.473777340600664 2314 100-101 8.557540221283782 2315 1 -0.6301298961006303 2315 2 -0.6074080229271495 2315 3 -0.5412597139313142 2315 4 -0.5230815693052548 2315 5 -0.8432117412607312 2315 6 -0.6195408404908278 2315 7 -0.6039140362489661 2315 8 -0.8409073006352799 2315 9 -1.272778859920244 2315 10-11 -0.8578860467068239 2315 12-13 -0.8827428824810895 2315 14-15 -0.7584576386636499 2315 16-17 -1.0089267221428315 2315 18-19 -0.824872266136154 2315 20-21 -0.992190543871736 2315 22-23 -0.9210152071590372 2315 24-25 -0.9957310282672367 2315 26-27 -1.1446448524116306 2315 28-29 -1.133465726902493 2315 30-31 -0.7547484841220466 2315 32-33 -0.7488143949675035 2315 34-35 -0.7836149918357691 2315 36-37 -1.1260097090806056 2315 38-39 -1.2880984261490127 2315 40-41 -1.0679318291388142 2315 42-43 -1.0594825158175283 2315 44-45 -1.0583315993622833 2315 46-47 -0.8969291996826172 2315 48-49 -0.8743307029583391 2315 50-51 -0.8270817059107429 2315 52-53 -0.924031496929139 2315 54-55 -0.8562048984868582 2315 56-57 0.12941803705836108 2315 58-59 3.5012579339894714 2315 60-61 3.1173929559585183 2315 62-63 0.9169758274891429 2315 64-65 0.9815575023310643 2315 66-67 1.6493297052692029 2315 68-69 5.182194952250292 2315 70-71 4.329415411614653 2315 72-73 8.29507665661237 2315 74-75 9.419940831494785 2315 76-77 8.376977730081752 2315 78-79 6.0570373018748995 2315 80-81 3.27144313788067 2315 82-83 1.533989519757725 2315 84-85 1.1790628911508172 2315 86-87 1.2679734605342254 2315 88-89 1.375703038738866 2315 90-91 2.242597833927281 2315 92-93 4.068349124888755 2315 94-95 7.9507901546393 2315 96-97 8.674349047281721 2315 98-99 8.875613551436752 2315 100-101 8.281670245299884 2316 1 -0.46490314414670664 2316 2 -0.43522052941715117 2316 3 -0.5821086405718141 2316 4 -0.2588156835154152 2316 5 -0.7783134698506515 2316 6 -0.5607120172749731 2316 7 -0.5312381485900062 2316 8 -0.6123519919881772 2316 9 -0.7907085671606566 2316 10-11 -0.653729677507144 2316 12-13 -0.674978938499315 2316 14-15 -0.9136837329642162 2316 16-17 -1.0916387461229462 2316 18-19 -1.0906022959728503 2316 20-21 -0.867249386540287 2316 22-23 -0.8911863008711762 2316 24-25 -2.388165764825139 2316 26-27 -1.62176197701271 2316 28-29 -1.1733826895131685 2316 30-31 -0.9025526141851188 2316 32-33 -1.0160751995198538 2316 34-35 -0.8619017010076178 2316 36-37 -0.9463050646633917 2316 38-39 -1.1159654486407575 2316 40-41 -0.95123643340456 2316 42-43 -1.268749543847406 2316 44-45 -1.0815208027710028 2316 46-47 -1.1720385778194498 2316 48-49 -1.1813431914494004 2316 50-51 -1.2021422792460186 2316 52-53 -1.055023930454226 2316 54-55 -1.1045702837678846 2316 56-57 -0.2508103071089458 2316 58-59 3.316755016085377 2316 60-61 3.1542457731378057 2316 62-63 0.6604105432790526 2316 64-65 0.5762941986959724 2316 66-67 5.457582216466616 2316 68-69 8.124351743732642 2316 70-71 5.019802596373706 2316 72-73 8.529825156124321 2316 74-75 9.470403611776259 2316 76-77 8.123749171311768 2316 78-79 5.579448570592064 2316 80-81 2.6045211978725007 2316 82-83 1.375298630679616 2316 84-85 1.1669378400961499 2316 86-87 0.9738154991211729 2316 88-89 1.063200010072105 2316 90-91 2.3994455629464078 2316 92-93 3.8560257394277393 2316 94-95 7.817326329035126 2316 96-97 8.670253424483993 2316 98-99 8.979881029439978 2316 100-101 7.91957323104611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 26.0 5 64.0 6 190.0 7 434.0 8 896.0 9 1590.0 10 2573.0 11 4164.0 12 5496.0 13 7456.0 14 9761.0 15 13201.0 16 17176.0 17 21622.0 18 22229.0 19 21039.0 20 21797.0 21 21239.0 22 20240.0 23 19777.0 24 20148.0 25 21690.0 26 28182.0 27 39865.0 28 54777.0 29 70979.0 30 82038.0 31 88142.0 32 91164.0 33 94077.0 34 93226.0 35 87043.0 36 68662.0 37 39139.0 38 11159.0 39 459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86974923807267 20.104168652625596 15.135322106650761 21.89076000265097 2 15.964820553597914 21.50588290824987 39.49309500338578 23.036201534766434 3 18.99407365586176 26.423016980260343 30.33218933439594 24.25072002948195 4 12.95257355043491 17.704022865918688 36.56704428592564 32.77635929772076 5 12.73884366876954 38.87282318257202 33.793052712268654 14.595280436389793 6 30.7639383693753 37.306315096780054 17.139576573101273 14.790169960743379 7 27.702779363671766 34.31485295359013 20.472857613546733 17.50951006919137 8 26.708340632479732 35.28605543841623 19.186513537382673 18.81909039172136 9 26.968659205048073 16.589046106703385 19.924627425850506 36.517667262398035 10-11 24.86133134615018 27.46016027563997 26.620660109083282 21.057848269126563 12-13 26.100567563470804 24.965576771741095 27.588731146645223 21.34512451814288 14-15 22.713350364178407 25.984171207645456 26.601701965052126 24.700776463124015 16-17 21.25730784486538 29.483029824992947 27.193987258186464 22.065675071955212 18-19 21.254900323405458 27.0739748010598 30.685986277732948 20.985138597801786 20-21 22.071257217078276 26.08436572762581 31.111563935530306 20.732813119765606 22-23 21.74004993977637 26.181213676346317 30.970784958524206 21.107951425353104 24-25 21.476781453118605 26.4625447138901 31.076664182687725 20.98400965030357 26-27 20.91618061115014 26.875103247086845 30.97821055325916 21.230505588503856 28-29 20.94484415299733 26.932832776091253 30.982871556872503 21.139451514038914 30-31 21.092600787464118 26.73270472365002 30.853978736199522 21.320715752686343 32-33 20.74932492228097 26.98100705711498 30.703396945698792 21.566271074905263 34-35 21.373093090149283 26.97062857339952 30.58230925001611 21.07396908643508 36-37 21.50436351668804 26.545887853649123 30.487102375251084 21.462646254411748 38-39 21.014827945445507 26.797930942129476 30.550288592264412 21.636952520160605 40-41 21.317197442976767 26.68059997984451 30.72172988559491 21.280472691583814 42-43 21.175155728380286 26.798627611020876 30.317939201243465 21.70827745935537 44-45 21.114254482330338 26.970881649047246 30.331176754709304 21.58368711391311 46-47 21.425526650883974 27.30268040956313 29.54011535135405 21.731677588198846 48-49 21.221470105832477 27.428874157273786 29.357628241241574 21.992027495652167 50-51 21.533013546964064 27.579191158680104 29.115010061604345 21.772785232751488 52-53 21.679980862781697 27.41529080083539 29.049988720116822 21.85473961626609 54-55 21.19323221108762 27.671570874687152 29.19376054743634 21.941436366788885 56-57 21.391484801300216 27.612584728854035 29.01433198167121 21.981598488174548 58-59 20.21135465147663 27.029946102322327 30.50223376642388 22.256465479777162 60-61 21.311036582229097 27.40687594947312 29.34062577249611 21.941461695801674 62-63 21.005115162638212 27.640530822360766 29.309323701161226 22.045030313839803 64-65 20.314916963833188 27.412867139860314 29.804108739082114 22.468107157224384 66-67 21.297327573738382 26.85917827199254 29.791854945172382 22.051639209096695 68-69 22.527832259269637 25.976930979732888 30.023676099747824 21.47156066124965 70-71 21.53523747432688 26.346529885763616 30.19743981589186 21.920792824017642 72-73 21.19548738876902 26.67224316366062 29.999486172247096 22.13278327532326 74-75 21.580952503976764 26.959778408482777 29.3509110570624 22.10835803047806 76-77 19.675418020507212 27.348841608346497 30.20201894986509 22.773721421281206 78-79 20.50024843757835 27.57091867202137 29.220453022505254 22.708379867895026 80-81 20.795453307724983 28.00156845090059 28.373213254862378 22.82976498651205 82-83 20.9623186195823 27.867765489466738 28.11991564134228 23.050000249608683 84-85 20.88543713642309 27.687485193700432 28.15148398832015 23.27559368155632 86-87 20.989317311872952 27.766547546539904 28.014439646760565 23.22969549482658 88-89 20.892516930237708 27.827220354951816 27.874328213795252 23.405934501015224 90-91 20.748494427368374 27.732741127026706 28.183082362863132 23.335682082741787 92-93 20.63721199859054 27.444900371688053 28.282619320958887 23.635268308762516 94-95 19.761065818693233 27.062691239126025 29.372167498676195 23.80407544350455 96-97 19.547827318961648 26.882695832014665 29.625963244007096 23.943513605016598 98-99 19.82500895690273 26.98819156807957 29.003620836531134 24.18317863848656 100-101 19.410079995333 27.320375110294385 29.056007671384716 24.213537222987902 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1364.0 1 982.0 2 589.5 3 626.0 4 717.0 5 775.0 6 970.0 7 1375.0 8 1798.0 9 2158.5 10 2263.5 11 2297.0 12 2518.5 13 2635.0 14 2688.0 15 2785.5 16 2827.5 17 2856.0 18 2923.5 19 3146.0 20 3516.5 21 3866.5 22 4318.5 23 4957.5 24 5663.0 25 6511.5 26 7667.5 27 9079.0 28 10620.0 29 12279.0 30 14133.5 31 16366.0 32 18849.0 33 21205.5 34 23195.0 35 25170.0 36 27608.0 37 29573.5 38 30962.5 39 32208.0 40 33513.0 41 34956.5 42 36237.0 43 37487.0 44 38665.5 45 39379.5 46 39764.0 47 39845.0 48 39763.5 49 39489.5 50 38521.5 51 37317.0 52 35978.0 53 34882.0 54 33444.0 55 31267.0 56 28498.5 57 25425.0 58 22544.0 59 19265.5 60 15841.0 61 12953.5 62 10377.5 63 7986.0 64 6123.0 65 4665.0 66 3427.0 67 2514.0 68 1802.0 69 1241.5 70 883.5 71 662.5 72 501.0 73 366.0 74 276.5 75 191.0 76 121.0 77 73.5 78 45.0 79 29.0 80 16.0 81 9.5 82 5.5 83 2.5 84 3.0 85 2.0 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022147047317529662 2 0.0055367618293824155 3 0.0032675971452092944 4 0.0 5 0.06290124504527891 6 1.8153317473384967E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.538329368346242E-5 16-17 0.0 18-19 0.0012707322231369476 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.076658736692484E-5 28-29 0.001951481628388884 30-31 0.008123609569339772 32-33 1.8153317473384967E-4 34-35 9.076658736692484E-4 36-37 0.02400776235855162 38-39 0.023553929421716996 40-41 0.026322310336408205 42-43 0.026322310336408205 44-45 0.026413076923775126 46-47 0.02809225879006324 48-49 0.02872762490163171 50-51 0.02382622918381777 52-53 0.018924833466003828 54-55 0.014159587629240274 56-57 0.14522653978707975 58-59 0.7923923077132538 60-61 0.3104671120885664 62-63 2.269164684173121E-4 64-65 0.15924997753526965 66-67 1.4735047793146578 68-69 2.816214906233577 70-71 2.2663055366710627 72-73 1.960422137244526 74-75 1.6275356780763293 76-77 1.1523272099167943 78-79 0.4422148136516578 80-81 2.722997621007745E-4 82-83 2.269164684173121E-4 84-85 0.0 86-87 0.0034491303199431435 88-89 0.0 90-91 0.23240784695301103 92-93 0.1828492902506701 94-95 1.3524675350608635 96-97 1.3410763283463145 98-99 1.0697296154128928 100-101 0.4232445968919705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1101727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.635608244527226 #Duplication Level Percentage of deduplicated Percentage of total 1 80.10209887654102 46.96835289289243 2 11.70053407952759 13.721358650778392 3 3.5037816807630775 6.1633911002272495 4 1.4963635599774356 3.5096074997689213 5 0.8001736330114853 2.3459333836430774 6 0.49189497373280655 1.7305536586349313 7 0.3331256575289096 1.3673117885745978 8 0.2444676148655042 1.1467605834982133 9 0.1647836302137209 0.86959695476905 >10 1.0453773269340594 11.39800095769296 >50 0.07559699954658661 3.0528651313746247 >100 0.0377765947673969 4.022136999387484 >500 0.003096440454186975 1.292355962961551 >1k 6.192880908373951E-4 1.0141127045689442 >5k 3.0964404541869754E-4 1.397661731227595 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8259 0.7496412450634322 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7137 0.6478011340377425 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4959 0.45011150675258027 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2932 0.26612763415982366 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1759 0.15965842717842077 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1521 0.13805597938509268 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 9.076658736692484E-5 0.0 7 9.076658736692484E-5 0.0 0.0 9.076658736692484E-5 0.0 8 9.076658736692484E-5 0.0 0.0 9.076658736692484E-5 0.0 9 9.076658736692484E-5 0.0 0.0 1.8153317473384967E-4 0.0 10-11 9.076658736692484E-5 0.0 0.0 1.8153317473384967E-4 0.0 12-13 9.076658736692484E-5 0.0 0.0 3.6306634946769935E-4 0.0 14-15 1.8153317473384967E-4 0.0 0.0 4.0844964315116174E-4 0.0 16-17 1.8153317473384967E-4 0.0 0.0 4.538329368346242E-4 0.0 18-19 1.8153317473384967E-4 0.0 0.0 8.168992863023235E-4 0.0 20-21 1.8153317473384967E-4 0.0 0.0 0.0011799656357700228 0.0 22-23 1.8153317473384967E-4 0.0 0.0 0.0019060983347054215 0.0 24-25 4.538329368346242E-4 0.0 0.0 0.0031768305578423694 0.0 26-27 4.538329368346242E-4 0.0 0.0 0.005718295004116265 0.0 28-29 6.807494052519362E-4 0.0 0.0 0.013932671160822963 0.0 30-31 7.261326989353987E-4 0.0 0.0 0.03880271609936037 0.0 32-33 7.261326989353987E-4 0.0 0.0 0.08581980835542743 0.0 34-35 7.261326989353987E-4 0.0 0.0 0.1531232328880022 0.0 36-37 7.261326989353987E-4 0.0 0.0 0.2664453172156079 0.0 38-39 7.261326989353987E-4 0.0 0.0 0.43427273725705184 0.0 40-41 7.261326989353987E-4 0.0 0.0 0.619572725366629 0.0 42-43 9.076658736692484E-4 0.0 0.0 0.7959775879142474 0.0 44-45 0.001089199048403098 0.0 0.0 1.0066922204865634 0.0 46-47 0.001406882104187335 0.0 0.0 1.254666537173002 0.0 48-49 0.0014522653978707974 0.0 0.0 1.5009616719931524 0.0 50-51 0.0014522653978707974 0.0 0.0 1.7486636889174905 0.0 52-53 0.0014522653978707974 0.0 0.0 1.9758070738032198 0.0 54-55 0.0014522653978707974 0.0 0.0 2.210211785678303 0.0 56-57 0.0015430319852377222 9.076658736692484E-5 0.0 2.421017184837986 0.0 58-59 0.0015430319852377222 9.076658736692484E-5 0.0 2.5611154124388347 0.0 60-61 0.0015430319852377222 9.076658736692484E-5 0.0 2.6692638012865255 0.0 62-63 0.001633798572604647 9.076658736692484E-5 0.0 2.7596219390102994 4.538329368346242E-5 64-65 0.001633798572604647 9.076658736692484E-5 0.0 2.8491631774477706 9.076658736692484E-5 66-67 0.001633798572604647 9.076658736692484E-5 0.0 2.9249078946054694 9.076658736692484E-5 68-69 0.001633798572604647 9.076658736692484E-5 0.0 3.016491381258697 9.076658736692484E-5 70-71 0.0016791818662881095 9.076658736692484E-5 0.0 3.1867694991590474 9.076658736692484E-5 72-73 0.0017699484536550345 9.076658736692484E-5 0.0 3.4012055618134074 9.076658736692484E-5 74-75 0.001860715041021959 9.076658736692484E-5 0.0 3.6307542612643604 9.076658736692484E-5 76-77 0.0019060983347054215 9.076658736692484E-5 0.0 3.9742604111544875 9.076658736692484E-5 78-79 0.0019060983347054215 9.076658736692484E-5 9.076658736692484E-5 4.480465668899827 9.076658736692484E-5 80-81 0.002087631509439271 9.076658736692484E-5 9.076658736692484E-5 5.05919342995134 9.076658736692484E-5 82-83 0.002178398096806196 9.076658736692484E-5 9.076658736692484E-5 5.595805494464599 9.076658736692484E-5 84-85 0.002178398096806196 9.076658736692484E-5 9.076658736692484E-5 6.0180516588955335 9.076658736692484E-5 86-87 0.002178398096806196 9.076658736692484E-5 9.076658736692484E-5 6.380936475188499 9.076658736692484E-5 88-89 0.002178398096806196 9.076658736692484E-5 9.076658736692484E-5 6.762836891534835 9.076658736692484E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3220 0.0 56.954887 1 GTATCAA 5970 0.0 41.22242 1 TGGTATC 1475 0.0 30.349518 2 TATCAAC 8060 0.0 30.28412 2 ATCAACG 8100 0.0 30.18863 3 TCAACGC 8200 0.0 29.705559 4 CAACGCA 8270 0.0 29.511093 5 GTGGTAT 1580 0.0 28.64517 1 AACGCAG 8535 0.0 28.574049 6 GTACATG 6650 0.0 26.443838 1 TACATGG 6605 0.0 26.182642 2 ACATGGG 6660 0.0 25.043217 3 ACGCAGA 9870 0.0 24.470667 7 CGCAGAG 9885 0.0 24.43353 8 GCAGAGT 10270 0.0 22.921612 9 GAGTACT 6440 0.0 21.822971 12-13 CATGGGA 4065 0.0 21.674152 4 AGTACTT 6585 0.0 21.306686 12-13 CATGGGG 2505 0.0 20.877396 4 AGAGTAC 9880 0.0 20.562193 10-11 >>END_MODULE