FastQCFastQC Report
Fri 27 May 2016
SRR939884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189640
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82880.6966813489795232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64060.5384822299182945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42610.35817558252916853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25980.21838539390067585No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.12760162738307387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.11087387781177499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.059.0307241
GTATCAA63250.044.6930851
GTACCGT952.100569E-839.6139036
TATCAAC81550.034.2601172
ATCAACG84250.033.386893
TCAACGC85300.033.1978874
CAACGCA86400.032.7207835
AACGCAG89100.031.7305816
TACCGTA953.729842E-529.7104287
ACGCAGA100150.027.8068647
GTACATG72550.027.2294121
CGCAGAG102000.027.1641678
TACATGG73050.026.9786472
GCAGAGT103000.025.7140669
TGGTATC13550.025.3401972
ACATGGG74350.024.7366283
GTGGTAT15400.023.823211
ATGGGAG18600.023.7726655
GAGTACT61600.023.71167212-13
CATGGGA45000.022.7880384
Produced by FastQC (version 0.11.5)