FastQCFastQC Report
Fri 27 May 2016
SRR939884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189640
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87000.7313136747251269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75920.6381762550015131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51930.4365186106721361No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31870.2678961702699976No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.15046568709861807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.13726841733633705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36400.058.527931
GTATCAA64000.041.442891
TGGTATC14400.032.286952
ATCAACG84050.031.2639753
TATCAAC84750.031.0682412
TCAACGC85900.030.5906524
CAACGCA87250.030.1173325
AACGCAG89700.029.3476076
GTGGTAT16950.028.272871
CTCGTAA856.448452E-427.9106081
GTACATG72600.027.0571421
TACATGG74250.025.8774812
ACGCAGA104650.025.1550927
CGCAGAG106750.024.6158038
ACATGGG74450.024.5283413
GCGGTCG1201.7275436E-423.716049
GTATAGG3500.023.0461861
GCAGAGT111850.022.9421149
GAGTACT67900.022.2141412-13
CATGGGA43550.022.1095564
Produced by FastQC (version 0.11.5)