##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939884_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1189640 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32240341615951 31.0 31.0 34.0 30.0 34.0 2 31.549266164553984 31.0 31.0 34.0 30.0 34.0 3 31.568375306815508 31.0 31.0 34.0 30.0 34.0 4 35.40655576476917 37.0 35.0 37.0 33.0 37.0 5 35.14634595339766 37.0 35.0 37.0 32.0 37.0 6 35.18995914730507 37.0 35.0 37.0 32.0 37.0 7 35.095259910561175 37.0 35.0 37.0 32.0 37.0 8 35.055258733734576 37.0 35.0 37.0 32.0 37.0 9 36.61276688746175 39.0 35.0 39.0 32.0 39.0 10-11 36.47457087858512 38.0 35.0 39.0 32.0 39.0 12-13 36.385490988870586 38.0 35.0 39.0 32.0 39.0 14-15 37.34105359604587 39.0 36.0 41.0 32.0 41.0 16-17 37.27054613160284 39.0 36.0 40.5 32.0 41.0 18-19 37.299896187081806 39.0 36.0 40.0 32.0 41.0 20-21 37.23734827342726 39.0 36.0 40.0 31.0 41.0 22-23 37.09899549443529 39.0 36.0 40.0 31.0 41.0 24-25 36.936373188527625 39.0 36.0 40.0 31.0 41.0 26-27 36.61097096600652 38.5 35.0 40.0 30.0 41.0 28-29 36.364272805218384 38.0 35.0 40.0 30.0 41.0 30-31 36.03749075350526 38.0 34.5 40.0 29.5 41.0 32-33 35.90994586597626 38.0 34.0 40.0 29.5 41.0 34-35 35.71368691368818 38.0 34.0 40.0 29.0 41.0 36-37 35.67931937392825 38.0 34.0 40.0 29.0 41.0 38-39 35.52911637133923 38.0 34.0 40.0 27.0 41.0 40-41 35.39629005413403 38.0 34.0 40.0 27.0 41.0 42-43 35.10154164284994 38.0 34.0 40.0 26.5 41.0 44-45 34.75922295820584 38.0 33.0 40.0 25.5 41.0 46-47 34.357768736760704 38.0 33.0 40.0 24.0 41.0 48-49 33.96103779294577 38.0 33.0 40.0 23.0 41.0 50-51 33.686971268619075 37.0 33.0 40.0 22.5 41.0 52-53 33.39899717561615 37.0 32.5 40.0 21.5 41.0 54-55 33.10488088833597 37.0 32.0 40.0 20.0 41.0 56-57 32.72157501429004 36.0 31.0 39.5 18.5 41.0 58-59 32.33116236844759 36.0 31.0 39.0 17.5 40.0 60-61 32.00840422312633 35.0 31.0 39.0 14.5 40.0 62-63 31.701665209643252 35.0 30.5 39.0 13.0 40.0 64-65 31.26488097239501 35.0 29.5 38.0 9.5 40.0 66-67 30.320434333075553 34.5 28.5 38.0 7.5 40.0 68-69 28.344737483608487 33.0 24.5 37.0 3.5 39.0 70-71 27.69103846541811 33.0 23.5 36.0 2.0 39.0 72-73 28.2756539793551 33.0 25.5 36.0 2.0 39.0 74-75 28.399760431727245 33.0 26.0 35.5 2.0 38.5 76-77 28.006176658484918 32.5 26.0 35.0 2.0 37.0 78-79 28.5429264315255 33.0 27.0 35.0 2.0 37.0 80-81 28.35691007363572 33.0 27.0 35.0 2.0 37.0 82-83 27.908818213913452 33.0 26.5 35.0 2.0 36.0 84-85 27.539888537708887 33.0 26.0 35.0 2.0 36.0 86-87 27.147590867825564 33.0 26.0 35.0 2.0 35.5 88-89 26.667601963619248 32.0 25.0 35.0 2.0 35.0 90-91 26.155164587606336 32.0 24.0 34.0 2.0 35.0 92-93 25.64925817894489 32.0 22.5 34.0 2.0 35.0 94-95 24.80003446420766 31.0 18.5 34.0 2.0 35.0 96-97 24.26699463703305 31.0 15.0 34.0 2.0 35.0 98-99 23.577205709290205 31.0 2.0 34.0 2.0 35.0 100-101 22.13331848290239 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3030206712587109 1101 2 0.34237085548499735 1101 3 0.2644742078916238 1101 4 0.13711602256799438 1101 5 0.3122516174907375 1101 6 0.22135822799164373 1101 7 0.20642668627456828 1101 8 0.23378284585762543 1101 9 0.38245853678928654 1101 10-11 0.49465121876970386 1101 12-13 0.5083142312812043 1101 14-15 0.4650609394288594 1101 16-17 0.5650669213138073 1101 18-19 0.45765253302657527 1101 20-21 0.6347880095374094 1101 22-23 0.44814185617440927 1101 24-25 0.2878625200008642 1101 26-27 0.196993364749126 1101 28-29 0.3387492745409588 1101 30-31 0.24604784297682158 1101 32-33 0.13971228688926374 1101 34-35 0.19380325548257105 1101 36-37 0.4074100793442099 1101 38-39 0.33970195142316584 1101 40-41 0.24774525646021317 1101 42-43 0.01250730571741343 1101 44-45 0.12029397695303601 1101 46-47 -0.06953756325939509 1101 48-49 0.1870793250488063 1101 50-51 0.19482487726552478 1101 52-53 -0.07437247902571187 1101 54-55 -0.028061449466541433 1101 56-57 -0.1481613430292157 1101 58-59 0.07448639569457782 1101 60-61 -0.2510767938222962 1101 62-63 -0.25606526291790743 1101 64-65 -0.3922463564225076 1101 66-67 -0.5456635723101861 1101 68-69 -8.547918419912694 1101 70-71 -6.444779560337711 1101 72-73 -1.886927902228539 1101 74-75 -0.9316628573310695 1101 76-77 -0.6934396127560127 1101 78-79 -0.763314300131757 1101 80-81 -0.7307202692387662 1101 82-83 -0.826133201090272 1101 84-85 -0.5016991017623127 1101 86-87 -0.6876835932181002 1101 88-89 -0.656205400896571 1101 90-91 -0.7510525418449383 1101 92-93 -0.8474052677108723 1101 94-95 -0.44149732147835863 1101 96-97 -0.6824808838547689 1101 98-99 -0.9445475397201193 1101 100-101 -0.5022489726840007 1102 1 0.22123548425083683 1102 2 0.19267228265035286 1102 3 0.20394886340343632 1102 4 0.2884078532766523 1102 5 0.28656264111278773 1102 6 0.25663750948771025 1102 7 0.25908416658952405 1102 8 0.18563446987337073 1102 9 0.33343663718299155 1102 10-11 0.30848611050198826 1102 12-13 0.27471740549380286 1102 14-15 0.6334572238776843 1102 16-17 0.4548922165893998 1102 18-19 0.4649729070423234 1102 20-21 0.38328702528544056 1102 22-23 0.32594082270984615 1102 24-25 0.29115668830794306 1102 26-27 0.4205600478199898 1102 28-29 0.28491684343859447 1102 30-31 0.28573472781933873 1102 32-33 0.23047569732234052 1102 34-35 0.1461716117817886 1102 36-37 0.2894536324938244 1102 38-39 0.46720256658063875 1102 40-41 0.3349592082318722 1102 42-43 0.38133420040245625 1102 44-45 0.3332799513624849 1102 46-47 0.5108881257169884 1102 48-49 0.5127806045763563 1102 50-51 0.520483095769471 1102 52-53 0.45038760955696233 1102 54-55 0.0526625908877989 1102 56-57 0.0761804384668423 1102 58-59 0.18989609057647527 1102 60-61 0.30100551424857613 1102 62-63 0.11636953038917852 1102 64-65 0.055471409325523524 1102 66-67 0.3347006009181577 1102 68-69 -4.481629049833952 1102 70-71 0.32517614832370256 1102 72-73 0.6600055377320899 1102 74-75 0.5254914241649971 1102 76-77 0.14555829570855394 1102 78-79 -0.06470148024986955 1102 80-81 0.15239673371398865 1102 82-83 0.02901259123255784 1102 84-85 0.11946846713532722 1102 86-87 -0.15011285010786324 1102 88-89 -0.2705447217627146 1102 90-91 -0.4153457259000568 1102 92-93 -0.3079398395612678 1102 94-95 -0.13765258722639118 1102 96-97 -0.4130357559020119 1102 98-99 0.0509802653979925 1102 100-101 -0.1761201587076222 1103 1 0.1741144212587109 1103 2 0.24783960548499806 1103 3 0.2215054578916238 1103 4 0.28711602256799296 1103 5 0.14584536749073607 1103 6 0.2033894779916423 1103 7 0.01423918627456544 1103 8 0.2564390958576226 1103 9 0.30042728678928654 1103 10-11 0.37121371876970954 1103 12-13 0.2774548562812029 1103 14-15 0.3924046894288651 1103 16-17 0.42483254631381584 1103 18-19 0.17601190802658095 1103 20-21 0.3058817595374066 1103 22-23 0.15517310617440927 1103 24-25 0.23434689500086137 1103 26-27 0.15871211474912883 1103 28-29 0.25515552454096024 1103 30-31 0.22807909297682016 1103 32-33 0.22564978688926374 1103 34-35 0.13755325548257957 1103 36-37 0.13006632934420992 1103 38-39 0.3775925764231687 1103 40-41 0.301260881460216 1103 42-43 0.23867918071741911 1103 44-45 0.3140439769530303 1103 46-47 0.1644468117406106 1103 48-49 0.011688700048800627 1103 50-51 0.18662175226553046 1103 52-53 0.1533618959742924 1103 54-55 0.19381355053345573 1103 56-57 0.36824490697078005 1103 58-59 0.4549551456945764 1103 60-61 0.2196263311777038 1103 62-63 0.0579972370820947 1103 64-65 0.13587864357749524 1103 66-67 0.23519580268981244 1103 68-69 -3.9162777949126912 1103 70-71 0.5751423146622905 1103 72-73 0.6513533477714581 1103 74-75 0.49607151766893054 1103 76-77 0.294060387243988 1103 78-79 0.05817007486824011 1103 80-81 0.05131098076122953 1103 82-83 0.24105429890972374 1103 84-85 0.2682227732376887 1103 86-87 0.3873164067819026 1103 88-89 0.17621647410343044 1103 90-91 0.13449433315506454 1103 92-93 0.12798535728912697 1103 94-95 0.18858080352163853 1103 96-97 -0.2895121338547675 1103 98-99 0.18474933527987858 1103 100-101 0.023922902315998584 1104 1 0.12923981157877407 1104 2 0.130123953650493 1104 3 0.0733631374388537 1104 4 0.15154688712693343 1104 5 0.06913100859144095 1104 6 0.17890665207439582 1104 7 0.09691263475232148 1104 8 0.07196773559221015 1104 9 0.14360278444736707 1104 10-11 0.1519837470679093 1104 12-13 0.023237569981439776 1104 14-15 0.04563367313300404 1104 16-17 0.16591952670022891 1104 18-19 0.045685563861866285 1104 20-21 0.09118194006043723 1104 22-23 0.0702882505928315 1104 24-25 0.07067410518821049 1104 26-27 -0.04250153669506318 1104 28-29 -0.15942658904217666 1104 30-31 -0.04648942039163728 1104 32-33 -0.04571078346592827 1104 34-35 0.0428268454513514 1104 36-37 0.1434994382435093 1104 38-39 0.11812304578303667 1104 40-41 0.13874746420806616 1104 42-43 0.37231135284856265 1104 44-45 0.21057561727309348 1104 46-47 0.21440930442211226 1104 48-49 0.16669364003709575 1104 50-51 0.01733963624288748 1104 52-53 0.04015169642315897 1104 54-55 0.04371688533049678 1104 56-57 0.14766894092863225 1104 58-59 0.2752225149373615 1104 60-61 0.1722530632229855 1104 62-63 0.37945447264025134 1104 64-65 0.3772226863175412 1104 66-67 0.24648611543376475 1104 68-69 -4.369049372293645 1104 70-71 0.2708064076560426 1104 72-73 0.5877711244108035 1104 74-75 0.5211407384925053 1104 76-77 0.4222491191916866 1104 78-79 -0.06797771006540287 1104 80-81 -0.12151328982815102 1104 82-83 0.030577559545946542 1104 84-85 0.11228350459990466 1104 86-87 -0.10955828828054948 1104 88-89 -0.18429197876542602 1104 90-91 -0.21856602008459447 1104 92-93 -0.3924966879880003 1104 94-95 -0.11506350512394903 1104 96-97 -0.7250800495456353 1104 98-99 -0.4384054876123926 1104 100-101 -0.5831376216886852 1105 1 0.08878552618716995 1105 2 0.09001965317975191 1105 3 0.022735088257284986 1105 4 0.06264016803699235 1105 5 0.02657991637786239 1105 6 0.07459377051947058 1105 7 -0.11286981611016955 1105 8 0.08535155611199485 1105 9 0.24846236230597896 1105 10-11 -0.016036629004545944 1105 12-13 0.1823448105737313 1105 14-15 0.11434489709976248 1105 16-17 0.07124488236309645 1105 18-19 0.01771066100749863 1105 20-21 0.14067533068207894 1105 22-23 0.18118661968792793 1105 24-25 0.12276228649529486 1105 26-27 0.18343637945501 1105 28-29 0.059013334556858865 1105 30-31 0.08371489881943006 1105 32-33 0.16283865314522217 1105 34-35 0.12012852376556538 1105 36-37 0.13239393268284516 1105 38-39 0.24275180734525748 1105 40-41 0.18596129878930867 1105 42-43 -0.022864440109295003 1105 44-45 0.20708476590374403 1105 46-47 0.2780851513670015 1105 48-49 0.11554464698838984 1105 50-51 0.11365561057236562 1105 52-53 -0.07319625684764475 1105 54-55 0.18111420136811773 1105 56-57 0.19874719234439198 1105 58-59 0.2691244620697759 1105 60-61 0.18033653586768494 1105 62-63 0.1539369727736677 1105 64-65 0.1505385700480808 1105 66-67 0.5768622623479978 1105 68-69 1.927131262122284 1105 70-71 2.258603786244162 1105 72-73 1.3309036011498385 1105 74-75 0.624449275021874 1105 76-77 0.4402330074745109 1105 78-79 0.5777224397331047 1105 80-81 0.5628639169694978 1105 82-83 0.36359441715296725 1105 84-85 0.29857290737918163 1105 86-87 0.13447273130495674 1105 88-89 0.394697570089118 1105 90-91 0.3427191940453618 1105 92-93 0.3773693000554239 1105 94-95 0.32316260499222693 1105 96-97 -0.3175924200232849 1105 98-99 0.17262629772026017 1105 100-101 0.13893060303142235 1106 1 0.4344577311178668 1106 2 0.47169523928781487 1106 3 0.41422112690570856 1106 4 0.3387225366524973 1106 5 0.3536248569273539 1106 6 0.3356077878507975 1106 7 0.2710393623309031 1106 8 0.18278152543508952 1106 9 0.35366232200055236 1106 10-11 0.2704544758119596 1106 12-13 0.3126111062812029 1106 14-15 0.6470426735837904 1106 16-17 0.5847192100462095 1106 18-19 0.46123968091390566 1106 20-21 0.4312228686923376 1106 22-23 0.525067472371596 1106 24-25 0.4348200464093068 1106 26-27 0.6324577133406777 1106 28-29 0.4206264752451858 1106 30-31 0.36100712994865347 1106 32-33 0.5894316971005296 1106 34-35 0.5549058082994733 1106 36-37 0.6710654490625245 1106 38-39 0.8870886151555624 1106 40-41 0.6937564800517677 1106 42-43 0.7370726666329048 1106 44-45 0.9471315649811984 1106 46-47 0.857421063501171 1106 48-49 0.8238971067389471 1106 50-51 0.8921290145894716 1106 52-53 0.714723031537666 1106 54-55 0.6079255663785332 1106 56-57 0.7650978999285272 1106 58-59 0.8182473992157071 1106 60-61 0.8993468417410817 1106 62-63 0.6752617617299812 1106 64-65 0.44680514005636596 1106 66-67 1.1734385403658685 1106 68-69 2.706571016707027 1106 70-71 2.8678524819158078 1106 72-73 1.8847435326306154 1106 74-75 1.385271561683016 1106 76-77 0.9069950263284916 1106 78-79 0.9408505326147214 1106 80-81 1.2501281022401045 1106 82-83 1.170087089402685 1106 84-85 1.2041217609137433 1106 86-87 0.9995468205142934 1106 88-89 1.3455221519203278 1106 90-91 1.1361888754085818 1106 92-93 1.2271931037679984 1106 94-95 1.6986545271131916 1106 96-97 1.3118082886804423 1106 98-99 1.405815576477064 1106 100-101 1.4141627790765625 1107 1 0.08111452296741817 1107 2 0.19290164779582142 1107 3 0.13467546399414587 1107 4 0.15682081304805706 1107 5 0.19533745963882865 1107 6 0.32420925016414515 1107 7 0.06375988399629051 1107 8 0.20818721424655706 1107 9 0.18041228475511417 1107 10-11 0.14095601433520244 1107 12-13 0.0807661103495505 1107 14-15 0.192972033305999 1107 16-17 0.25766125613886715 1107 18-19 0.07784298257905675 1107 20-21 0.20787842857728123 1107 22-23 0.0600652949457654 1107 24-25 0.22654138747441976 1107 26-27 0.16277156348794364 1107 28-29 0.088405371977899 1107 30-31 0.045683280832804485 1107 32-33 0.24498048003409423 1107 34-35 0.09733445462822488 1107 36-37 -0.07709269221803794 1107 38-39 0.08093180038573422 1107 40-41 -0.10869512952432103 1107 42-43 0.08300510626664703 1107 44-45 0.31501338805962575 1107 46-47 0.23190034357543965 1107 48-49 0.19466043774205133 1107 50-51 0.39156066347382534 1107 52-53 0.3629921212590759 1107 54-55 0.3858113002283332 1107 56-57 0.524834359777941 1107 58-59 0.4762020944333969 1107 60-61 0.4238110087611098 1107 62-63 0.6522831148282258 1107 64-65 0.5836022755953927 1107 66-67 0.8278667368842783 1107 68-69 2.290003671218308 1107 70-71 2.6203572378722164 1107 72-73 1.562574042950466 1107 74-75 1.052379682335328 1107 76-77 0.8292938722928085 1107 78-79 0.6756321883751575 1107 80-81 0.6186685250045194 1107 82-83 0.7920354700854801 1107 84-85 1.00717180446226 1107 86-87 0.737862137558956 1107 88-89 0.7469062498357282 1107 90-91 0.7559637696888295 1107 92-93 0.7578572043192331 1107 94-95 1.1624508833629719 1107 96-97 1.0173125203356328 1107 98-99 1.0332666130463544 1107 100-101 1.1214583732273091 1108 1 -0.13455207671699654 1108 2 -0.19044480747046677 1108 3 -0.16419165749299225 1108 4 -0.21338372439556963 1108 5 -0.028892107205621187 1108 6 0.05627409337625977 1108 7 0.09058660530291007 1108 8 0.09680473350944396 1108 9 -0.11321770308925494 1108 10-11 0.11899648698832976 1108 12-13 -0.06760144432608683 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2316 68-69 0.9126451236865627 2316 70-71 0.9592498923524566 2316 72-73 0.08888885913509625 2316 74-75 -0.33459798024387055 2316 76-77 -0.4284559914665884 2316 78-79 -0.684637786912834 2316 80-81 -0.6654293659456343 2316 82-83 -0.6607248928806264 2316 84-85 -0.9695721884970041 2316 86-87 -0.6796688958618837 2316 88-89 -0.7816793688928634 2316 90-91 -0.8904563857688714 2316 92-93 -0.8015369061617115 2316 94-95 -0.7078861781573949 2316 96-97 -0.6207505383074583 2316 98-99 -0.6531094019650148 2316 100-101 -1.1999218912832603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 24.0 5 83.0 6 186.0 7 477.0 8 953.0 9 1738.0 10 2815.0 11 4389.0 12 5746.0 13 7333.0 14 9188.0 15 11467.0 16 14087.0 17 16271.0 18 14194.0 19 11632.0 20 10766.0 21 11132.0 22 11894.0 23 13182.0 24 15726.0 25 18726.0 26 23195.0 27 28099.0 28 34462.0 29 41024.0 30 48348.0 31 57238.0 32 68056.0 33 82241.0 34 101528.0 35 127124.0 36 151551.0 37 155514.0 38 83110.0 39 6130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.828640372634254 20.08634823477976 15.20948064941944 21.87553074316655 2 16.046031014686744 21.413821747947654 39.39600057845653 23.144146658909072 3 19.010961299216568 26.368649339295917 30.347247906929827 24.27314145455768 4 12.96123196933526 17.761507682996537 36.56198513836119 32.715275209307016 5 12.750243771224909 38.772569853064795 33.84208668168522 14.635099694025083 6 30.82380453229929 37.25054407261362 17.155960757843346 14.769690637243734 7 27.684509599542718 34.34013651188595 20.43895632292122 17.536397565650113 8 26.680928684307858 35.23872768232407 19.219511785077838 18.86083184829024 9 26.96521636797687 16.59989576678659 19.990249151003663 36.44463871423288 10-11 24.857519921993205 27.46957062640799 26.639487576073435 21.033421875525367 12-13 26.114244645438955 24.929180256211964 27.57119800948186 21.38537708886722 14-15 22.633149524225814 26.03413637739148 26.574047611041994 24.758666487340708 16-17 21.2851393806937 29.488184425928065 27.178909541772178 22.047766651606057 18-19 21.30333546282909 27.04486231128745 30.635654483709356 21.016147742174105 20-21 22.101139840624054 26.104031471705724 31.052125012608855 20.742703675061364 22-23 21.74304831713796 26.150179886352177 30.98521401432366 21.12155778218621 24-25 21.428466354723426 26.479786523564492 31.081852947888834 21.009894173823245 26-27 20.995993313924043 26.817855706343913 30.940417514089386 21.245733465642658 28-29 20.973925703511735 26.973098561832625 30.96090494679478 21.092070787860855 30-31 21.128395576462044 26.74305417791661 30.792107838027764 21.336442407593584 32-33 20.779376891890415 26.985576029932325 30.594258488236132 21.64078858994113 34-35 21.49100851338373 26.990936009868882 30.54259949268555 20.975455984061835 36-37 21.59391296482116 26.521054081969663 30.453393244434345 21.43163970877483 38-39 21.01996841468473 26.89728546343499 30.493543688952794 21.589202432927483 40-41 21.327055493334388 26.6766267282502 30.682010378546853 21.314307399868564 42-43 21.19094541195161 26.76023567980936 30.318278817090654 21.730540091148377 44-45 21.06184064607219 27.06622105321614 30.256025794422094 21.61591250628958 46-47 21.44617380571567 27.32599277947965 29.54250406164786 21.685329353156813 48-49 21.23776385872229 27.41101781726044 29.331611891411175 22.019606432606096 50-51 21.47045596906836 27.59166265970528 29.089487962919726 21.84839340830663 52-53 21.69181850300308 27.45028516630495 29.029593364625917 21.82830296606605 54-55 21.21013185679073 27.715748166272537 29.137856857179745 21.936263119756987 56-57 21.330138008597945 27.803361397534132 28.83162530613103 22.03487528773689 58-59 21.234320879712143 27.296717893533305 28.96740592527827 22.501555301476277 60-61 21.05697652424287 27.699585969189382 29.125827536201427 22.117609970366324 62-63 21.0404957607813 27.667942159000543 29.216564139723573 22.074997940494583 64-65 20.78249053127024 27.477326957939123 29.190377895943374 22.549804614847254 66-67 20.681676379279423 27.60967000692976 29.154173308039283 22.554480305751532 68-69 21.0463745698084 27.25405199112429 29.40218143868904 22.297392000378263 70-71 20.959970489198927 27.30833806749836 29.08936893650535 22.64232250679736 72-73 20.80889583276306 27.625510876591587 28.752608339527374 22.81298495111798 74-75 20.903165970442423 27.972364606246508 28.328212553562178 22.796256869748888 76-77 20.879946049214958 27.711413752741265 28.530471766652767 22.878168431391018 78-79 20.988411412306323 27.785331826353183 28.44388607532687 22.782370686013625 80-81 20.97967376991234 27.99766816392286 28.187518624637015 22.835139441527783 82-83 21.034724790692984 27.851703036212637 28.080847987626512 23.032724185467874 84-85 20.910235260741192 27.709617425476246 28.12251631274233 23.25763100104023 86-87 20.97116668950288 27.75684224772219 27.95492888130857 23.31706218146635 88-89 20.8851768353062 27.89289683080063 27.790008389106603 23.431917944786566 90-91 20.835898908021363 27.76406435785259 27.99514470681838 23.40489202730766 92-93 20.865938858388414 27.52406483193076 28.031336610067765 23.578659699613063 94-95 20.565065864056923 27.253101804820286 28.31883125214271 23.86300107898008 96-97 20.825052041821785 26.987594036797518 28.250385899777037 23.93696802160366 98-99 20.669299034364126 27.13650772832385 28.0217264918677 24.172466745444325 100-101 20.490235269045545 27.08609970235656 28.33938834503773 24.084276683560173 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2177.0 1 1414.5 2 873.5 3 1119.5 4 953.0 5 875.5 6 1385.0 7 2559.5 8 3774.5 9 4202.5 10 3506.0 11 2603.5 12 2376.5 13 2370.5 14 2466.5 15 2596.0 16 2600.5 17 2669.0 18 2845.5 19 3028.5 20 3331.5 21 3745.0 22 4291.0 23 5004.5 24 5797.5 25 6721.5 26 7961.5 27 9647.0 28 11340.5 29 13220.0 30 15223.5 31 17372.0 32 20432.0 33 22646.5 34 23887.5 35 25830.0 36 28763.0 37 31397.0 38 32862.0 39 33789.0 40 34423.5 41 35605.5 42 36480.0 43 37977.5 44 39817.5 45 40855.5 46 41106.0 47 41319.5 48 41824.0 49 41079.0 50 40293.5 51 39275.0 52 37639.0 53 37159.0 54 37188.0 55 35150.0 56 31850.5 57 29025.0 58 26658.5 59 23812.0 60 19861.0 61 16485.5 62 13804.5 63 10618.0 64 8044.0 65 6298.0 66 4693.5 67 3523.0 68 2586.0 69 1737.0 70 1241.5 71 938.0 72 721.0 73 579.5 74 441.5 75 317.0 76 232.5 77 149.0 78 84.5 79 60.5 80 43.5 81 28.0 82 16.0 83 9.5 84 7.0 85 6.5 86 3.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02269594162940049 2 0.022527823543256786 3 0.0 4 0.0 5 0.0 6 8.405904307185368E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1014760767963414E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.202952153592684E-5 26-27 2.942066507514879E-4 28-29 8.405904307185368E-5 30-31 3.362361722874147E-4 32-33 0.0019333579906526343 34-35 0.003572509330553781 36-37 0.10192158972462259 38-39 0.09355771493897314 40-41 0.10314044584916444 42-43 0.10490568575367337 44-45 0.09801284422178139 46-47 0.09103594364681752 48-49 0.09259103594364682 50-51 0.09107797316835345 52-53 0.07229077704179415 54-55 0.06190948522242023 56-57 0.0338337648364211 58-59 0.013449446891496585 60-61 0.009666789953263172 62-63 0.002858007464443025 64-65 0.005589926364278269 66-67 0.16887461753135402 68-69 0.44416798359167475 70-71 0.41937056588547794 72-73 0.19690830839581724 74-75 0.03093372785044215 76-77 0.0342960895733163 78-79 0.005421808278134562 80-81 0.0016391513399011464 82-83 0.0 84-85 2.1014760767963414E-4 86-87 7.56531387646683E-4 88-89 1.6811808614370735E-4 90-91 8.826199522544635E-4 92-93 0.003446420765946 94-95 0.08553007632561112 96-97 0.01782051713123298 98-99 0.018198782825056322 100-101 0.01088564607780505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1189640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.8055408595008 #Duplication Level Percentage of deduplicated Percentage of total 1 79.42098368891695 45.909729177314354 2 11.964995896404181 13.832861183467028 3 3.6845044294715295 6.389543140344846 4 1.6385892335897445 3.788781475768403 5 0.8570837866373691 2.4772095924241055 6 0.5042062957888855 1.748755057970518 7 0.3445932486660763 1.3943579380972497 8 0.2380069107918453 1.1006494565297262 9 0.17589470426382492 0.9150919662863108 >10 1.0459009986384815 11.273555375746094 >50 0.08052069539036978 3.178333990988982 >100 0.04065465349664056 4.258984647481371 >500 0.00319428838471427 1.3741136268332796 >1k 4.3558477973376403E-4 0.554782010344978 >5k 4.3558477973376403E-4 1.8032513604027005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8700 0.7313136747251269 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7592 0.6381762550015131 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5193 0.4365186106721361 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3187 0.2678961702699976 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1790 0.15046568709861807 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1633 0.13726841733633705 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.405904307185368E-5 0.0 5 8.405904307185368E-5 0.0 0.0 8.405904307185368E-5 0.0 6 8.405904307185368E-5 0.0 0.0 8.405904307185368E-5 0.0 7 8.405904307185368E-5 0.0 0.0 8.405904307185368E-5 8.405904307185368E-5 8 8.405904307185368E-5 0.0 0.0 8.405904307185368E-5 8.405904307185368E-5 9 8.405904307185368E-5 0.0 0.0 2.52177129215561E-4 8.405904307185368E-5 10-11 1.260885646077805E-4 0.0 0.0 3.782656938233415E-4 8.405904307185368E-5 12-13 2.101476076796342E-4 0.0 0.0 4.623247368951952E-4 8.405904307185368E-5 14-15 3.362361722874147E-4 0.0 0.0 5.04354258431122E-4 8.405904307185368E-5 16-17 3.362361722874147E-4 0.0 0.0 6.724723445748294E-4 8.405904307185368E-5 18-19 3.782656938233415E-4 0.0 0.0 8.826199522544635E-4 8.405904307185368E-5 20-21 4.2029521535926834E-4 0.0 0.0 0.0010507380383981708 8.405904307185368E-5 22-23 4.2029521535926834E-4 0.0 0.0 0.001765239904508927 8.405904307185368E-5 24-25 5.884133015029757E-4 0.0 0.0 0.0036985978951615617 8.405904307185368E-5 26-27 5.884133015029757E-4 0.0 0.0 0.006850812010356075 8.405904307185368E-5 28-29 9.246494737903904E-4 0.0 0.0 0.014626273494502538 8.405904307185368E-5 30-31 0.0010927675599340977 0.0 0.0 0.03845701220537305 8.405904307185368E-5 32-33 0.0010927675599340977 0.0 0.0 0.08632863723479373 8.405904307185368E-5 34-35 0.0011768266030059513 0.0 0.0 0.1516004841800881 8.405904307185368E-5 36-37 0.0013449446891496588 0.0 0.0 0.2627265391210787 8.405904307185368E-5 38-39 0.0014290037322215125 0.0 0.0 0.43702296493056725 8.405904307185368E-5 40-41 0.0014290037322215125 0.0 0.0 0.6288457012205373 8.405904307185368E-5 42-43 0.001555092296829293 0.0 0.0 0.8107914999495646 8.405904307185368E-5 44-45 0.001765239904508927 0.0 0.0 1.0240072627013215 8.405904307185368E-5 46-47 0.002017417033724488 0.0 0.0 1.2750916243569483 8.405904307185368E-5 48-49 0.0021014760767963415 0.0 0.0 1.5444167983591675 8.405904307185368E-5 50-51 0.0021014760767963415 0.0 0.0 1.8386234491106555 8.405904307185368E-5 52-53 0.0021014760767963415 0.0 0.0 2.112193604788003 8.405904307185368E-5 54-55 0.0021014760767963415 0.0 0.0 2.3992552368783837 8.405904307185368E-5 56-57 0.0021855351198681954 0.0 0.0 2.725446353518712 8.405904307185368E-5 58-59 0.0021855351198681954 0.0 0.0 3.0662637436535425 8.405904307185368E-5 60-61 0.0021855351198681954 0.0 4.202952153592684E-5 3.4216653777613395 8.405904307185368E-5 62-63 0.0021855351198681954 0.0 8.405904307185368E-5 3.752900036985979 8.405904307185368E-5 64-65 0.0021855351198681954 0.0 8.405904307185368E-5 4.086572408459702 8.405904307185368E-5 66-67 0.0023536532060119027 0.0 8.405904307185368E-5 4.438611680844625 8.405904307185368E-5 68-69 0.0025638008136915367 0.0 8.405904307185368E-5 4.832428297636259 8.405904307185368E-5 70-71 0.002647859856763391 0.0 8.405904307185368E-5 5.288322517736458 8.405904307185368E-5 72-73 0.0026898893782993176 0.0 8.405904307185368E-5 5.751824081234659 8.405904307185368E-5 74-75 0.0026898893782993176 0.0 8.405904307185368E-5 6.206793651861068 8.405904307185368E-5 76-77 0.0026898893782993176 0.0 8.405904307185368E-5 6.693789717897852 8.405904307185368E-5 78-79 0.0026898893782993176 0.0 8.405904307185368E-5 7.25194176389496 8.405904307185368E-5 80-81 0.002858007464443025 0.0 8.405904307185368E-5 7.850778386738845 8.405904307185368E-5 82-83 0.002858007464443025 0.0 8.405904307185368E-5 8.448648330587405 8.405904307185368E-5 84-85 0.0029000369859789516 0.0 8.405904307185368E-5 9.016341077973168 8.405904307185368E-5 86-87 0.0029420665075148783 0.0 8.405904307185368E-5 9.573064120238055 8.405904307185368E-5 88-89 0.0029420665075148783 0.0 8.405904307185368E-5 10.168790558488283 1.260885646077805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3640 0.0 58.52793 1 GTATCAA 6400 0.0 41.44289 1 TGGTATC 1440 0.0 32.28695 2 ATCAACG 8405 0.0 31.263975 3 TATCAAC 8475 0.0 31.068241 2 TCAACGC 8590 0.0 30.590652 4 CAACGCA 8725 0.0 30.117332 5 AACGCAG 8970 0.0 29.347607 6 GTGGTAT 1695 0.0 28.27287 1 CTCGTAA 85 6.448452E-4 27.910608 1 GTACATG 7260 0.0 27.057142 1 TACATGG 7425 0.0 25.877481 2 ACGCAGA 10465 0.0 25.155092 7 CGCAGAG 10675 0.0 24.615803 8 ACATGGG 7445 0.0 24.528341 3 GCGGTCG 120 1.7275436E-4 23.71604 9 GTATAGG 350 0.0 23.046186 1 GCAGAGT 11185 0.0 22.942114 9 GAGTACT 6790 0.0 22.21414 12-13 CATGGGA 4355 0.0 22.109556 4 >>END_MODULE