FastQCFastQC Report
Fri 27 May 2016
SRR939883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791984
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79990.21094498289022318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57500.15163566090996164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45250.053.156911
GTATCAA88200.044.5381431
ATCAACG112450.035.693323
TCAACGC114550.035.1227344
TATCAAC116350.034.537222
CAACGCA117600.034.1315775
AACGCAG122500.032.8825046
ACGCAGA139900.028.453627
CGCAGAG142300.027.8070188
TACATGG107900.024.8865872
GTACATG108550.024.7812711
GCAGAGT156250.023.9276189
ACATGGG112550.022.6784233
AGAGTAC140100.021.11418710-11
GAGTACT98450.020.93913312-13
CAGAGTA146400.020.57826210-11
TGGTATC22700.020.2729152
GTGGTAT24850.019.8553371
AGTACTT104300.019.8329312-13
GTACTTT105550.019.4652614-15
Produced by FastQC (version 0.11.5)