##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939883_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3791984 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.680013417778135 31.0 28.0 33.0 16.0 34.0 2 29.528192893218957 31.0 30.0 33.0 25.0 34.0 3 29.784520451563086 31.0 30.0 33.0 25.0 34.0 4 33.5459461326841 35.0 33.0 37.0 28.0 37.0 5 33.48761044350398 35.0 33.0 37.0 28.0 37.0 6 33.50031355617534 35.0 33.0 37.0 28.0 37.0 7 33.40221451356335 35.0 33.0 37.0 27.0 37.0 8 33.25660366710408 35.0 33.0 37.0 26.0 37.0 9 34.50925452217098 37.0 34.0 39.0 27.0 39.0 10-11 34.33030993801661 37.0 33.0 39.0 26.5 39.0 12-13 34.20426905809729 37.0 33.0 39.0 26.0 39.0 14-15 34.85429988628644 38.0 33.0 40.0 25.0 41.0 16-17 34.671109239912404 38.0 33.0 40.0 25.0 41.0 18-19 34.47198142186254 38.0 33.0 40.0 25.0 41.0 20-21 34.645245074873735 38.0 33.0 40.0 25.0 41.0 22-23 34.53191798805058 38.0 33.0 40.0 24.5 41.0 24-25 34.31598841134351 38.0 32.5 40.0 23.5 41.0 26-27 34.191603392841316 38.0 32.5 40.0 23.5 41.0 28-29 34.2407055251288 38.0 33.0 40.0 23.5 41.0 30-31 34.017356613319045 38.0 32.5 40.0 22.0 41.0 32-33 33.661861838024635 38.0 32.0 40.0 18.5 41.0 34-35 33.47374276895683 38.0 31.5 40.0 18.5 41.0 36-37 33.264666332980305 37.5 31.0 40.0 18.0 41.0 38-39 33.02081918067165 37.0 31.0 40.0 16.5 41.0 40-41 32.78248708327883 37.0 31.0 40.0 15.5 41.0 42-43 32.377460980848014 37.0 30.0 40.0 14.5 41.0 44-45 32.0380494748923 37.0 30.0 39.5 10.5 41.0 46-47 31.656784944240272 36.0 30.0 39.0 9.0 41.0 48-49 31.292996093865376 36.0 29.5 39.0 8.0 40.0 50-51 30.66279986940873 35.5 28.5 38.5 7.0 40.0 52-53 30.813021099245145 35.5 29.0 39.0 6.5 40.0 54-55 30.89752910349833 36.0 29.0 39.0 2.0 40.0 56-57 30.567116316946485 35.5 28.0 39.0 2.0 40.0 58-59 30.142437046147872 35.0 28.0 39.0 2.0 40.0 60-61 29.705001524268035 34.5 27.0 38.0 2.0 40.0 62-63 29.23070508736324 34.0 26.0 38.0 2.0 40.0 64-65 28.666164862509966 34.0 26.0 38.0 2.0 40.0 66-67 28.051752723640185 33.5 25.0 37.0 2.0 39.0 68-69 27.484300830383248 33.0 23.5 36.5 2.0 39.0 70-71 26.89577909611433 32.0 22.0 36.0 2.0 39.0 72-73 26.21866983088536 32.0 20.5 35.5 2.0 38.0 74-75 25.850181066164836 32.0 20.0 35.0 2.0 38.0 76-77 25.28104839049954 31.0 18.0 35.0 2.0 37.0 78-79 24.60993005244748 31.0 15.0 34.5 2.0 36.5 80-81 23.876413508073874 30.0 7.5 34.0 2.0 36.0 82-83 23.12079415419474 29.5 4.5 34.0 2.0 35.5 84-85 22.329150518567587 29.0 2.0 34.0 2.0 35.0 86-87 21.423241764733184 29.0 2.0 34.0 2.0 35.0 88-89 20.436369061683806 27.5 2.0 33.5 2.0 35.0 90-91 19.050964745631838 25.5 2.0 32.5 2.0 34.5 92-93 17.542355400233756 21.5 2.0 32.0 2.0 34.0 94-95 16.981626504753187 19.5 2.0 31.0 2.0 34.0 96-97 16.60473250941987 18.5 2.0 31.0 2.0 34.0 98-99 15.687467695011371 2.0 2.0 31.0 2.0 34.0 100-101 14.111281983257314 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6441545401105806 1101 2 1.0152980890530188 1101 3 0.8193836410703454 1101 4 0.8032116894807544 1101 5 1.0819252412630718 1101 6 1.0178863857294544 1101 7 1.0052524201878583 1101 8 1.1438132449246368 1101 9 1.1490560861113295 1101 10-11 1.1190443406151829 1101 12-13 1.1614493098930438 1101 14-15 1.4174838095821087 1101 16-17 1.331613975242142 1101 18-19 1.1157910996240403 1101 20-21 1.129733529112876 1101 22-23 1.294142632316131 1101 24-25 1.5010588837533803 1101 26-27 1.3984186200438629 1101 28-29 1.3115104338689463 1101 30-31 1.4713800719118524 1101 32-33 1.5222310498390428 1101 34-35 1.3844986115674303 1101 36-37 1.330721208231104 1101 38-39 1.3531435889305783 1101 40-41 1.3430289797452133 1101 42-43 1.5010621366101304 1101 44-45 1.344153377043341 1101 46-47 1.0926595801262131 1101 48-49 1.132385980985692 1101 50-51 0.9527780987959034 1101 52-53 0.9267328745174304 1101 54-55 1.0583776864358896 1101 56-57 1.1935858141545914 1101 58-59 1.1778951594761438 1101 60-61 0.9453661464918888 1101 62-63 0.6380754099807966 1101 64-65 0.8353327105386299 1101 66-67 0.893812150036954 1101 68-69 0.8478372933894818 1101 70-71 0.6762080017129044 1101 72-73 0.7940419086133019 1101 74-75 0.5761596463951761 1101 76-77 0.5040280392995022 1101 78-79 0.19799930134726296 1101 80-81 -0.036786893563924394 1101 82-83 -0.11737524165937785 1101 84-85 -0.12718421123000567 1101 86-87 -0.49742840795276777 1101 88-89 -4.353631195974522 1101 90-91 -7.8170397059308705 1101 92-93 -10.231698462232316 1101 94-95 -8.411520796920469 1101 96-97 -5.326725329681782 1101 98-99 -3.961767414918951 1101 100-101 -2.9304154189954854 1102 1 1.8930832304481129 1102 2 1.1955653195216414 1102 3 0.9697715725397096 1102 4 1.0227166662810916 1102 5 1.0400444490555572 1102 6 1.0792268652376364 1102 7 1.0937654784734292 1102 8 1.2062671286673918 1102 9 1.4844718022403356 1102 10-11 1.3951848456827847 1102 12-13 1.3760082024975162 1102 14-15 1.6231504340234153 1102 16-17 1.6175423371186994 1102 18-19 1.6227423652077206 1102 20-21 1.615352039065911 1102 22-23 1.6709802481602836 1102 24-25 1.7653944116843334 1102 26-27 1.7413456109041334 1102 28-29 1.5340152795091058 1102 30-31 1.6569678096677194 1102 32-33 1.881844755069416 1102 34-35 1.922085645318191 1102 36-37 1.8081362091447417 1102 38-39 1.8119479125083586 1102 40-41 1.6174501093146603 1102 42-43 1.582953567365962 1102 44-45 1.6248042790778925 1102 46-47 1.6968981279924016 1102 48-49 1.364907653244419 1102 50-51 1.4436218847738687 1102 52-53 1.6331045690285428 1102 54-55 1.6154611211287602 1102 56-57 1.6004947049093694 1102 58-59 1.6112298567259273 1102 60-61 1.51494740919453 1102 62-63 1.493569452281637 1102 64-65 1.3887045362099322 1102 66-67 1.4910517326112576 1102 68-69 1.5536090701839882 1102 70-71 1.3391861898862913 1102 72-73 1.4068392750961571 1102 74-75 1.2549607974659232 1102 76-77 1.3138523294930913 1102 78-79 1.1829322471041195 1102 80-81 0.9720794788704659 1102 82-83 0.7022665565131234 1102 84-85 0.6777294752792109 1102 86-87 0.46491068260268875 1102 88-89 -0.18835086630283726 1102 90-91 -5.59585789661536 1102 92-93 -10.126895701366479 1102 94-95 -8.020874423835883 1102 96-97 -4.799295587260206 1102 98-99 -3.3188240570612777 1102 100-101 -2.471792309467764 1103 1 2.069014038196361 1103 2 1.2307441890021558 1103 3 1.0911573921861617 1103 4 0.9445072812792787 1103 5 0.8190763297019501 1103 6 0.6673306085257096 1103 7 0.70068322657675 1103 8 0.8064104046100127 1103 9 0.9038132770885241 1103 10-11 0.8606034127834263 1103 12-13 0.868960316614519 1103 14-15 0.9694283691395498 1103 16-17 0.9419824686869092 1103 18-19 0.989941736441672 1103 20-21 0.7187749192155479 1103 22-23 0.8716553087396548 1103 24-25 0.7629155746216583 1103 26-27 0.82240244006681 1103 28-29 0.7924709312125202 1103 30-31 0.831191511380112 1103 32-33 0.8000814969915808 1103 34-35 0.8884954051852247 1103 36-37 0.9251600809535887 1103 38-39 0.7436824454311193 1103 40-41 0.7348023963267707 1103 42-43 0.7874114859855439 1103 44-45 0.7459929096692832 1103 46-47 0.7458530609838796 1103 48-49 0.7695075650865739 1103 50-51 0.605638134115992 1103 52-53 0.6617013743559532 1103 54-55 0.5491649791619722 1103 56-57 0.5202760361240095 1103 58-59 0.694054351858977 1103 60-61 0.5959549119407193 1103 62-63 0.5489358865297937 1103 64-65 0.513581025817178 1103 66-67 0.4160689715913328 1103 68-69 0.5378627486752663 1103 70-71 0.45578357671345415 1103 72-73 0.7203168924086789 1103 74-75 0.3917138365406885 1103 76-77 0.43039106103066516 1103 78-79 0.2947426004429623 1103 80-81 0.1435121761042204 1103 82-83 -0.3541863027476104 1103 84-85 -0.5080697253918451 1103 86-87 -0.4477209225309906 1103 88-89 -1.3342894247898194 1103 90-91 -6.853601677560981 1103 92-93 -9.847237192540199 1103 94-95 -8.760236437753008 1103 96-97 -5.7419719165663174 1103 98-99 -4.321473495890691 1103 100-101 -3.6287464790464643 1104 1 1.9548682946402671 1104 2 1.1723536589778547 1104 3 0.9871226234919526 1104 4 0.8090313451995641 1104 5 0.9439877462352015 1104 6 0.856341674562934 1104 7 0.7968789414689823 1104 8 0.9170124097212735 1104 9 0.8381004023894576 1104 10-11 0.9150801126998189 1104 12-13 0.7351421140080348 1104 14-15 0.946919040391009 1104 16-17 0.86617487664072 1104 18-19 0.997767797557664 1104 20-21 0.8997178604025251 1104 22-23 0.756046855258397 1104 24-25 0.7662686204137472 1104 26-27 0.7850658580733736 1104 28-29 0.7112051784136213 1104 30-31 0.8121228790821817 1104 32-33 0.8095820154996929 1104 34-35 0.7817831437409382 1104 36-37 0.9359199571939243 1104 38-39 0.7900186612307039 1104 40-41 0.6911827179643808 1104 42-43 0.9072318018024248 1104 44-45 0.8332214058411722 1104 46-47 0.9154706109444213 1104 48-49 0.7728622432930514 1104 50-51 0.7304400789411858 1104 52-53 0.6160629324228637 1104 54-55 0.6927464856792369 1104 56-57 0.6913533976413007 1104 58-59 0.6685728629051582 1104 60-61 0.6584038240331367 1104 62-63 0.6289500772080565 1104 64-65 0.7073390529650965 1104 66-67 0.5744592750764888 1104 68-69 0.45816704537650565 1104 70-71 0.3708257689620318 1104 72-73 0.2930333578863831 1104 74-75 0.12981959887021333 1104 76-77 0.22556653025319306 1104 78-79 -0.044929986535407807 1104 80-81 -0.09795789234524577 1104 82-83 -0.4363059415322823 1104 84-85 -0.39843694134041385 1104 86-87 -0.18443117044209956 1104 88-89 -0.593050492892548 1104 90-91 -3.5655894758963704 1104 92-93 -7.963667483381808 1104 94-95 -5.6702006552295146 1104 96-97 -3.231260579668234 1104 98-99 -2.325594042964058 1104 100-101 -1.8155906648927793 1105 1 1.9821032960076863 1105 2 1.3236750655273148 1105 3 1.0708679971975776 1105 4 0.9513353265220132 1105 5 0.9658837692691264 1105 6 0.9163979201914358 1105 7 0.8339471125367979 1105 8 0.8585011978398782 1105 9 0.9755967082112136 1105 10-11 0.892733695723301 1105 12-13 0.9127290942583386 1105 14-15 1.0283342918411904 1105 16-17 0.86055770887004 1105 18-19 0.8992160633309041 1105 20-21 0.911775873320515 1105 22-23 0.8646950240081921 1105 24-25 1.0167622755701728 1105 26-27 0.8581250732967973 1105 28-29 0.7955895878116692 1105 30-31 0.8976231387166962 1105 32-33 1.0108146273153125 1105 34-35 0.9250115099743113 1105 36-37 0.9334914803839567 1105 38-39 0.9577221516274577 1105 40-41 0.9029216197655217 1105 42-43 0.9019041513858355 1105 44-45 0.8928377114800696 1105 46-47 1.0304446985865958 1105 48-49 0.7151477167488842 1105 50-51 0.7226236818184333 1105 52-53 0.7265333159370577 1105 54-55 0.7736874436351187 1105 56-57 0.799055265154287 1105 58-59 0.7269913337694867 1105 60-61 0.7591985530357483 1105 62-63 0.7892445048947998 1105 64-65 0.7834493770604958 1105 66-67 0.6368429538407021 1105 68-69 0.6074701667184641 1105 70-71 0.4674061431207761 1105 72-73 0.44199667533508347 1105 74-75 0.3181688396913742 1105 76-77 0.30924860880292115 1105 78-79 0.11820028598805266 1105 80-81 -0.034442631994085815 1105 82-83 -0.2805619414290206 1105 84-85 -0.24140555877521308 1105 86-87 -0.08858492597261147 1105 88-89 -0.5116647545315303 1105 90-91 -3.4693397626237648 1105 92-93 -8.165755201661018 1105 94-95 -5.641749163319329 1105 96-97 -3.162786952268899 1105 98-99 -2.1038928601009577 1105 100-101 -1.4756121469258279 1106 1 1.6889778056364477 1106 2 1.077852614116381 1106 3 0.8792191896652497 1106 4 0.8478955434778399 1106 5 0.8159643776623469 1106 6 0.7743486972428641 1106 7 0.7887722110043214 1106 8 -0.20770042904790387 1106 9 -0.27462247526607797 1106 10-11 -0.6107342630373367 1106 12-13 -0.33470211892958446 1106 14-15 0.5845599453394286 1106 16-17 0.7085513337981624 1106 18-19 0.947582960319302 1106 20-21 0.7261161261378746 1106 22-23 0.7058114776434152 1106 24-25 0.7102651533979625 1106 26-27 0.7673206453551344 1106 28-29 0.48752928222734226 1106 30-31 0.6329041360078378 1106 32-33 0.7751649649604033 1106 34-35 0.79156662552424 1106 36-37 0.7399412684761586 1106 38-39 0.8487665788050265 1106 40-41 0.6396184257404656 1106 42-43 0.6644639405089947 1106 44-45 0.7388957658162241 1106 46-47 0.6497986347454265 1106 48-49 0.48487142056058374 1106 50-51 0.5957533536008341 1106 52-53 0.6209846732172188 1106 54-55 0.6102313478904975 1106 56-57 0.5483004832183234 1106 58-59 0.5059513632992072 1106 60-61 0.4143832640998184 1106 62-63 0.6161097134470737 1106 64-65 0.48432815462206236 1106 66-67 0.5084258405903306 1106 68-69 0.504819191369549 1106 70-71 0.6214303615957881 1106 72-73 0.4958105380685005 1106 74-75 0.4306414610695768 1106 76-77 0.28497363487879923 1106 78-79 0.08344081334307774 1106 80-81 0.016615249579697178 1106 82-83 -0.22251231059270538 1106 84-85 -0.23084275515939723 1106 86-87 0.12238134800378475 1106 88-89 -0.4085148751702299 1106 90-91 -3.8066074780001475 1106 92-93 -8.348632315928297 1106 94-95 -5.876792823038702 1106 96-97 -3.153670155637176 1106 98-99 -2.007811560096437 1106 100-101 -1.463420320580381 1107 1 1.8449139361226976 1107 2 1.135414276722532 1107 3 0.8233168392344297 1107 4 0.939693964498332 1107 5 0.9201226296593177 1107 6 0.8696399647904229 1107 7 0.7595002244177493 1107 8 0.7411554186270166 1107 9 0.7907074796882512 1107 10-11 0.8326538933279082 1107 12-13 0.7411096667805026 1107 14-15 0.9075415310077233 1107 16-17 0.8827349964636895 1107 18-19 0.932099244490793 1107 20-21 0.8034893290865455 1107 22-23 0.7310687214485796 1107 24-25 0.9258737097795589 1107 26-27 0.8646959266455312 1107 28-29 0.6953194783925483 1107 30-31 0.7724660428611543 1107 32-33 0.9021713209887352 1107 34-35 0.9098234290122704 1107 36-37 0.6850278809864321 1107 38-39 0.6231661565263238 1107 40-41 0.648271693918808 1107 42-43 0.8090702376043026 1107 44-45 0.6332632076688753 1107 46-47 0.5984921644923169 1107 48-49 0.6577421146538889 1107 50-51 0.6797434980035675 1107 52-53 0.6566117778183092 1107 54-55 0.5262570991606097 1107 56-57 0.50787876751642 1107 58-59 0.3775120858898866 1107 60-61 0.14326386700938443 1107 62-63 0.2002740100012268 1107 64-65 0.3636149444916299 1107 66-67 0.7099914453233502 1107 68-69 0.6848244516468363 1107 70-71 0.6246319741872881 1107 72-73 0.5688713920260575 1107 74-75 0.5153178503793967 1107 76-77 0.4375270681480643 1107 78-79 0.4458808509436949 1107 80-81 0.38715794885821353 1107 82-83 0.2925661706156646 1107 84-85 0.475268381783156 1107 86-87 0.6093821188388802 1107 88-89 0.5847642662897776 1107 90-91 -2.800621391685663 1107 92-93 -4.350779422187578 1107 94-95 -2.6149027897236117 1107 96-97 -1.1800645352633765 1107 98-99 -0.6799499328389 1107 100-101 -0.25983918654526583 1108 1 1.5614161274051135 1108 2 0.9716998979821057 1108 3 -0.5155647281526754 1108 4 -0.21251708597351637 1108 5 0.2968446468794923 1108 6 0.5262529605422301 1108 7 0.48537105245376466 1108 8 0.5372593029851913 1108 9 0.5911156318790134 1108 10-11 0.5996075197969404 1108 12-13 0.41533916485278866 1108 14-15 0.4239791854052726 1108 16-17 0.46014030360499447 1108 18-19 0.49051197305085736 1108 20-21 0.3581834668892654 1108 22-23 0.28873640106655074 1108 24-25 0.2558694073205743 1108 26-27 0.41207239568332454 1108 28-29 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3.809290813444587 2311 94-95 3.3008884961826404 2311 96-97 2.6741395167688857 2311 98-99 2.6417863955896213 2311 100-101 2.4877781953263227 2312 1 1.1521946674585486 2312 2 0.6028267829162175 2312 3 -0.13390575971541452 2312 4 -0.6950121047662563 2312 5 -0.04713148860934524 2312 6 -0.26016362687796146 2312 7 0.07398559757896805 2312 8 0.2370481677227687 2312 9 0.21492184027461292 2312 10-11 0.22975457862047932 2312 12-13 0.14653369231735525 2312 14-15 0.14913230092399488 2312 16-17 -0.0070168421632388345 2312 18-19 -0.3159412375636421 2312 20-21 0.37171678437330513 2312 22-23 0.3824974389690112 2312 24-25 0.41612871312141664 2312 26-27 0.5094920737162028 2312 28-29 0.38915744547561815 2312 30-31 0.3002312878511191 2312 32-33 0.4300376128486292 2312 34-35 0.31412346030027294 2312 36-37 0.3567755413861633 2312 38-39 0.3612896796289249 2312 40-41 0.3184564757732318 2312 42-43 0.5314781114685942 2312 44-45 0.3563031723146395 2312 46-47 0.4628954827254397 2312 48-49 0.6350116821367671 2312 50-51 0.35935437015979943 2312 52-53 0.2745088220147487 2312 54-55 0.266346531727649 2312 56-57 0.5173247019625684 2312 58-59 0.43810672845789256 2312 60-61 0.3679253904309512 2312 62-63 0.19965665014897738 2312 64-65 0.24072515010374929 2312 66-67 0.3555681490283611 2312 68-69 0.4906160808267366 2312 70-71 0.6030789811911781 2312 72-73 0.4374592681060747 2312 74-75 0.4943475204187564 2312 76-77 0.6840564125243205 2312 78-79 0.6954283922785649 2312 80-81 0.8740661702993897 2312 82-83 0.8196708369114205 2312 84-85 1.2424665590437165 2312 86-87 1.5300287944577313 2312 88-89 1.7980640431344597 2312 90-91 2.643389024663964 2312 92-93 3.497112837500442 2312 94-95 3.0344058305483372 2312 96-97 2.2818387251417107 2312 98-99 2.0156093007145035 2312 100-101 1.917218182510771 2313 1 -6.962246298997954 2313 2 -2.5079427855375833 2313 3 -1.4791322212656226 2313 4 -0.744317037359032 2313 5 -0.6465208260314341 2313 6 -0.2748955567159115 2313 7 -0.03675799200148333 2313 8 0.013203909477923048 2313 9 -0.01912649527487531 2313 10-11 -0.022747717063644757 2313 12-13 -0.07307134397884596 2313 14-15 -0.18262597043116813 2313 16-17 -0.5486045648658049 2313 18-19 -0.49518692772157635 2313 20-21 -0.3726141483000518 2313 22-23 -1.013717280130443 2313 24-25 -0.17071765312343956 2313 26-27 -0.23927901953840802 2313 28-29 -0.49165074136867304 2313 30-31 -0.011702982418242414 2313 32-33 -0.27954599352796805 2313 34-35 -0.1513056046511494 2313 36-37 -0.1900564675548111 2313 38-39 -0.23258004851541614 2313 40-41 -0.11513627516679037 2313 42-43 -0.10473039390849692 2313 44-45 -0.24064812737127994 2313 46-47 -1.2871297008762745 2313 48-49 -0.7857830275042623 2313 50-51 -0.7375960812363829 2313 52-53 -1.061758003282101 2313 54-55 -0.8074162907019797 2313 56-57 -0.5440481065339036 2313 58-59 -0.43680791238683625 2313 60-61 -0.19076809491590296 2313 62-63 -0.303364957911473 2313 64-65 -0.3267038639502893 2313 66-67 -0.06562509773700498 2313 68-69 -0.15161289590255578 2313 70-71 0.0011210224307838246 2313 72-73 -0.08334323519695275 2313 74-75 0.032678764838600216 2313 76-77 0.31869213570192656 2313 78-79 0.16024462090961578 2313 80-81 0.3798895733409289 2313 82-83 0.7989675835081869 2313 84-85 0.6160619502855482 2313 86-87 1.157498484004897 2313 88-89 1.6324525958885374 2313 90-91 2.3163649523218126 2313 92-93 3.449756850589555 2313 94-95 3.194944134011269 2313 96-97 2.2258137472502604 2313 98-99 1.791889635025493 2313 100-101 1.6465123312202063 2314 1 0.6154186167602838 2314 2 0.10127817585554055 2314 3 -1.3244426496412132 2314 4 -0.2660133619515719 2314 5 -0.06989576609986159 2314 6 -3.367085011133849 2314 7 -2.700156317105364 2314 8 -2.319252563163584 2314 9 -3.195723988244051 2314 10-11 -2.403567409126289 2314 12-13 -1.2547305938199145 2314 14-15 -1.127090886939527 2314 16-17 -2.185077281386789 2314 18-19 -3.182544678132423 2314 20-21 -2.8615007086870214 2314 22-23 -3.911970028544097 2314 24-25 -3.0326050617727134 2314 26-27 -1.9762457379009462 2314 28-29 -1.8991743655053313 2314 30-31 -2.006762807727455 2314 32-33 -2.1966084273844473 2314 34-35 -1.5889459007990894 2314 36-37 -2.0196231755406657 2314 38-39 -3.2968026320461306 2314 40-41 -2.6316204279140116 2314 42-43 -1.6905460040603941 2314 44-45 -2.2891396488113145 2314 46-47 -1.8312034759436138 2314 48-49 -2.3691651968679253 2314 50-51 -3.156720098811725 2314 52-53 -2.0969843305438225 2314 54-55 -2.5986840646728915 2314 56-57 -1.6721175877811802 2314 58-59 -0.9973881094312311 2314 60-61 -1.4580027597772762 2314 62-63 -1.0717252737375986 2314 64-65 -1.7287944992468773 2314 66-67 -1.208571674714154 2314 68-69 -1.0479877622090399 2314 70-71 -1.0054626995592137 2314 72-73 -2.022516201320844 2314 74-75 -2.0940759723344797 2314 76-77 -1.1838902354444016 2314 78-79 -2.0886437929023742 2314 80-81 -0.9760783614685167 2314 82-83 -0.5528232688860442 2314 84-85 -1.6802317935142277 2314 86-87 -0.3365526623994306 2314 88-89 0.8848833799967153 2314 90-91 1.9193459973784854 2314 92-93 2.9097713154310476 2314 94-95 2.762320978137911 2314 96-97 2.1679076549515734 2314 98-99 2.2301258870187812 2314 100-101 2.362816173529678 2315 1 -3.005684174254011 2315 2 -2.062673579219428 2315 3 -2.8375864244051385 2315 4 -4.238443237499215 2315 5 -4.086597167716171 2315 6 -3.385369907539914 2315 7 -5.936866446095955 2315 8 -3.227604660351627 2315 9 -2.667945454093836 2315 10-11 -4.094209918420944 2315 12-13 -3.1775313827745464 2315 14-15 -2.2728422265914787 2315 16-17 -2.6966309285565018 2315 18-19 -2.172405563786363 2315 20-21 -1.4655969890801046 2315 22-23 -0.8269318899184839 2315 24-25 -2.2338003629544048 2315 26-27 -3.964618780787262 2315 28-29 -3.233700556355558 2315 30-31 -3.288626276684191 2315 32-33 -4.022626475594464 2315 34-35 -3.894980356472754 2315 36-37 -3.0674345246181822 2315 38-39 -1.9114764900692052 2315 40-41 -1.7180828740224747 2315 42-43 -3.0995729018279334 2315 44-45 -6.607655995183343 2315 46-47 -3.5486610172677295 2315 48-49 -2.5354108549992205 2315 50-51 -3.4817407826887745 2315 52-53 -3.3673189380738044 2315 54-55 -2.0325916592339937 2315 56-57 -1.4008983735939573 2315 58-59 -2.7217343418237547 2315 60-61 -2.9875026812309073 2315 62-63 -4.938176548806982 2315 64-65 -4.240251796204518 2315 66-67 -3.5701453948447153 2315 68-69 -1.870870588481928 2315 70-71 -3.5158419912455194 2315 72-73 -2.6394169361657625 2315 74-75 -1.7746415061879652 2315 76-77 -3.163719528003444 2315 78-79 -1.8669580132860268 2315 80-81 -1.5902306716044912 2315 82-83 -1.2103405054502474 2315 84-85 -2.6165872420139955 2315 86-87 -2.0046055851970763 2315 88-89 -1.4550869469307237 2315 90-91 -0.5443599948366966 2315 92-93 -0.05872984530973113 2315 94-95 -0.05676858217697145 2315 96-97 -0.329233606328561 2315 98-99 -0.5807138609346474 2315 100-101 -1.1177846189159055 2316 1 -7.0837113235752796 2316 2 -5.919535396135544 2316 3 -3.3480549242311035 2316 4 -4.001566105581361 2316 5 -2.1325867256695297 2316 6 -2.890011745340125 2316 7 -3.1624132612717304 2316 8 -2.428279912701086 2316 9 -6.79585495635628 2316 10-11 -6.601186597850116 2316 12-13 -5.950959854755055 2316 14-15 -4.739898265575118 2316 16-17 -4.822080284630296 2316 18-19 -3.968316458321695 2316 20-21 -2.8593018272283786 2316 22-23 -3.4807341871687427 2316 24-25 -2.9976158867970746 2316 26-27 -3.7937952513754993 2316 28-29 -4.094172536863738 2316 30-31 -6.820421612570023 2316 32-33 -4.697078268145805 2316 34-35 -5.272575901199524 2316 36-37 -4.111712979195282 2316 38-39 -6.200794276355317 2316 40-41 -3.7241100233437407 2316 42-43 -4.461816549582892 2316 44-45 -2.9892390094541383 2316 46-47 -4.007740028753982 2316 48-49 -5.132983427223376 2316 50-51 -4.798253835247074 2316 52-53 -6.241687890388459 2316 54-55 -5.138025700306041 2316 56-57 -4.334954064509379 2316 58-59 -2.9636104295369456 2316 60-61 -3.1851831546036884 2316 62-63 -4.122931856847355 2316 64-65 -3.014418172814331 2316 66-67 -3.9414597004486147 2316 68-69 -3.061456735366715 2316 70-71 -2.600574326783285 2316 72-73 -2.079054249079089 2316 74-75 -2.261676661078326 2316 76-77 -2.957788445511273 2316 78-79 -4.930211058882964 2316 80-81 -5.750163146369541 2316 82-83 -3.373861563577641 2316 84-85 -1.9774386168834681 2316 86-87 -1.975587833717789 2316 88-89 -1.0726755302930648 2316 90-91 -0.8294494483695942 2316 92-93 -0.8220128247737044 2316 94-95 -1.4575705313590106 2316 96-97 -2.8516401500111215 2316 98-99 -4.334401295671855 2316 100-101 -3.7978987852925155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35326.0 3 12657.0 4 12293.0 5 13462.0 6 15009.0 7 17525.0 8 20685.0 9 24777.0 10 28737.0 11 33552.0 12 38001.0 13 41580.0 14 44863.0 15 48640.0 16 53314.0 17 57065.0 18 54952.0 19 55920.0 20 60075.0 21 65558.0 22 72465.0 23 81016.0 24 90583.0 25 100526.0 26 113846.0 27 127551.0 28 143430.0 29 162067.0 30 182939.0 31 206316.0 32 229117.0 33 253210.0 34 287061.0 35 328333.0 36 344156.0 37 261703.0 38 72502.0 39 1172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97854258250065 19.122441903545663 13.397761569051209 23.50125394490248 2 15.81353446668874 19.31129463491244 40.422190995364026 24.452979903034798 3 19.718691437508564 23.39436617114949 29.030069813553265 27.856872577788682 4 13.017573029055285 15.97024014501235 36.61316202552505 34.39902480040732 5 14.241893423377656 36.792625209523095 34.05369494704756 14.91178642005169 6 33.28989959623526 35.47634018915595 17.131070500772328 14.102689713836462 7 29.374932473074423 31.844416906813766 20.318544815556724 18.462105804555083 8 26.479630890617962 35.34074947081045 19.251194803561987 18.928424835009604 9 26.539142967410424 16.693958707173532 19.60845277508409 37.158445550331955 10-11 25.394717900155978 25.967581531536887 27.25289601551057 21.384804552796567 12-13 26.28447836767056 23.168580922833772 27.46246794111188 23.084472768383783 14-15 23.007558714246002 24.751022014499366 25.483451982321842 26.757967288932793 16-17 22.92170299957451 27.837929849610575 26.168052871492197 23.072314279322715 18-19 22.88383396455683 26.588546843167105 28.135631052894 22.39198813938207 20-21 23.423183765185403 25.945373836275255 28.263676386810925 22.367766011728417 22-23 23.13238821600935 25.833799972569803 28.135690145557092 22.89812166586376 24-25 22.845827089957215 25.95921120553965 28.359654411455686 22.835307293047443 26-27 22.6344914038221 26.3020098106081 28.162673374847074 22.900825410722724 28-29 22.685454175378865 26.534730280913877 28.116288352396634 22.663527191310624 30-31 22.88662025494115 26.223395833834427 27.997429813476458 22.89255409774797 32-33 22.658697512447674 26.213482143742134 28.01811892277652 23.109701421033677 34-35 23.083800683979344 26.45210823153811 27.819260837161114 22.644830247321433 36-37 23.207288319692466 26.068955801537946 27.8444905784404 22.879265300329184 38-39 22.85877610823222 26.35287631902204 27.840482638910913 22.94786493383483 40-41 23.191902106403784 26.236104870893566 27.848587361577295 22.723405661125355 42-43 22.892168204498283 26.18544334648933 27.813594960931738 23.108793488080654 44-45 22.950150368220797 26.411202548061212 27.894655965887733 22.74399111783026 46-47 23.164776091001354 26.5663983212111 27.370351086772665 22.898474501014885 48-49 22.979985526991737 26.63071857666398 27.316943810409413 23.072352085934874 50-51 22.95449221424972 26.820649860423895 27.27229582202368 22.95256210330271 52-53 23.156158718806168 26.601310516188438 27.176890601048758 23.06564016395664 54-55 22.810685943714788 26.713999994976444 27.373566089535316 23.10174797177345 56-57 22.760532360307888 26.8405436843109 27.27268762285678 23.12623633252443 58-59 22.847802426189684 26.589093446697376 27.237451899471132 23.325652227641804 60-61 22.62405211541836 26.67863039530513 27.457853885718997 23.239463603557514 62-63 22.711817964064263 26.625539320791052 27.51993622416456 23.14270649098013 64-65 22.489465742408022 26.643040968957482 27.402228890758828 23.465264397875668 66-67 22.50144947298378 26.72489927682265 27.27367741205782 23.499973838135755 68-69 22.71222433747446 26.447086219489897 27.458778126483157 23.38191131655249 70-71 22.67387967845366 26.396187533901838 27.188491246801416 23.741441540843084 72-73 22.674989356292635 26.585013580722922 27.11984913627025 23.620147926714193 74-75 22.65551204988885 26.79976386918268 26.9925123901266 23.55221169080187 76-77 22.73883686416086 26.497414510417443 27.097099497655915 23.666649127765783 78-79 22.88262413288291 26.594256754702332 26.961766075293937 23.56135303712082 80-81 22.8844866519281 26.610970205504763 26.820387242365413 23.684155900201723 82-83 22.921076012629108 26.652916382405984 26.85614293949346 23.56986466547145 84-85 22.933685642265438 26.61716012388091 26.801289567011615 23.64786466684203 86-87 22.71344536359958 26.491945381907485 27.053171834276068 23.74143742021687 88-89 22.86784615167009 26.477723813115624 26.815061335837555 23.839368699376735 90-91 22.666987686046227 26.457823960070698 27.046611113362484 23.82857724052059 92-93 22.974236813577825 26.071277857335534 27.24871205287221 23.70577327621443 94-95 22.770243961749244 26.07969714381501 27.166575003119032 23.983483891316713 96-97 23.145834534463216 25.8745063025599 26.75713180234013 24.222527360636757 98-99 23.026792237048817 26.024611170568292 26.518601762974725 24.429994829408166 100-101 23.291160140518215 26.00356971198211 26.54245307294625 24.162817074553423 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4719.0 1 2681.0 2 614.0 3 554.5 4 467.5 5 418.5 6 537.0 7 884.5 8 1370.0 9 1669.0 10 1653.0 11 1561.0 12 1660.5 13 1860.5 14 2084.5 15 2338.0 16 2613.5 17 2972.5 18 3413.5 19 3992.0 20 4773.5 21 5685.5 22 6918.0 23 8699.0 24 10973.5 25 13863.0 26 18235.5 27 23367.5 28 27957.5 29 32596.0 30 37924.5 31 45277.0 32 54476.0 33 60817.5 34 65216.5 35 71130.5 36 78744.5 37 86869.0 38 92758.5 39 97998.0 40 104120.5 41 110188.0 42 115835.5 43 121688.5 44 127387.0 45 132084.5 46 135534.0 47 139630.5 48 142524.0 49 143064.5 50 143184.5 51 142104.0 52 141449.5 53 141999.5 54 140195.5 55 133276.5 56 124226.5 57 114459.0 58 104804.0 59 93345.5 60 78656.0 61 65078.0 62 53384.0 63 41686.0 64 31206.0 65 23466.5 66 17391.5 67 12804.0 68 9419.5 69 7052.5 70 5339.5 71 3970.0 72 3029.5 73 2278.0 74 1715.0 75 1284.0 76 947.5 77 676.0 78 468.0 79 331.0 80 208.5 81 139.5 82 103.0 83 72.0 84 45.5 85 23.5 86 23.5 87 21.5 88 16.0 89 14.5 90 11.0 91 8.0 92 5.5 93 4.5 94 4.0 95 3.5 96 4.0 97 2.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13541723804741793 2 0.12252161401524903 3 0.12914084025670994 4 0.13903012248996832 5 0.12689926961717138 6 0.14169363583812591 7 0.17028025434706476 8 0.15838674424786603 9 0.16843425499685652 10-11 0.16294899978480923 12-13 0.15262458913328747 14-15 0.15708135899307593 16-17 0.1515565466520956 18-19 0.1673662125156646 20-21 0.17308881050131014 22-23 0.2065673272883008 24-25 0.2278754340735615 26-27 0.24103477229861728 28-29 0.23665711669669495 30-31 0.22682057730201394 32-33 0.22802047687964927 34-35 0.21740598061595195 36-37 0.22575253482082203 38-39 0.2141359246241545 40-41 0.21846083738749955 42-43 0.21177568259781687 44-45 0.20499822784062377 46-47 0.2189882657732733 48-49 0.25767513786978 50-51 0.2589145945763484 52-53 0.26072103679762365 54-55 0.25845309473879635 56-57 0.23390130338102694 58-59 0.2344814746053781 60-61 0.20485318503453603 62-63 0.19721865915046055 64-65 0.19898554424280268 66-67 0.20701564141620848 68-69 0.20643547019185737 70-71 0.21312062498154002 72-73 0.24347412858282103 74-75 0.24617719905991164 76-77 0.26147262224735124 78-79 0.2575696521926253 80-81 0.2715069472866974 82-83 0.27014881919332995 84-85 0.24575525635129264 86-87 0.24959229785779685 88-89 0.2523876683023979 90-91 0.4354185038755438 92-93 0.5758726830071014 94-95 0.3366179815104705 96-97 0.2853914995421922 98-99 0.31520438904805503 100-101 0.3345873822252415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3791984.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.85700556460791 #Duplication Level Percentage of deduplicated Percentage of total 1 75.79065927588705 42.33439276918525 2 12.868160551526206 14.375538310657346 3 4.688243963366433 7.856138074499949 4 2.2706956730689085 5.073370433845645 5 1.2185299431799574 3.403171690842212 6 0.7393875515566688 2.4779984749021597 7 0.4923343430394067 1.9250225497159805 8 0.35127912166395797 1.5697119882811423 9 0.2508180452269579 1.260895045315164 >10 1.22500051972126 12.034195390064328 >50 0.07067884910250437 2.7337927093406456 >100 0.03137278121133528 3.009489799783258 >500 0.0018287541696409937 0.7251753478616146 >1k 9.14377062666328E-4 0.851517184152242 >5k 9.625021712277134E-5 0.3695902315531513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7999 0.21094498289022318 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5750 0.15163566090996164 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2742838577377965E-5 2 0.0 0.0 0.0 0.0 5.2742838577377965E-5 3 0.0 0.0 0.0 0.0 5.2742838577377965E-5 4 0.0 0.0 0.0 0.0 5.2742838577377965E-5 5 0.0 0.0 0.0 0.0 5.2742838577377965E-5 6 0.0 0.0 0.0 0.0 1.582285157321339E-4 7 2.6371419288688983E-5 0.0 0.0 0.0 1.582285157321339E-4 8 2.6371419288688983E-5 0.0 0.0 0.0 1.582285157321339E-4 9 2.6371419288688983E-5 0.0 0.0 0.0 1.582285157321339E-4 10-11 2.6371419288688983E-5 0.0 0.0 0.0 1.582285157321339E-4 12-13 2.6371419288688983E-5 0.0 0.0 3.9557128933033474E-5 3.691998700416458E-4 14-15 2.6371419288688983E-5 0.0 0.0 5.2742838577377965E-5 4.4831412790771275E-4 16-17 2.6371419288688983E-5 0.0 0.0 1.318570964434449E-4 5.933569339955021E-4 18-19 2.6371419288688983E-5 0.0 0.0 2.637141928868898E-4 6.4609977257288E-4 20-21 2.6371419288688983E-5 0.0 0.0 3.1645703146426774E-4 7.515854497276359E-4 22-23 2.6371419288688983E-5 0.0 0.0 4.8787125684074617E-4 8.438854172380474E-4 24-25 2.6371419288688983E-5 0.0 0.0 6.856569015059135E-4 8.966282558154254E-4 26-27 2.6371419288688983E-5 0.0 0.0 0.0013844995126561714 9.229996751041144E-4 28-29 7.911425786606695E-5 0.0 0.0 0.004760041181608362 9.493710943928033E-4 30-31 7.911425786606695E-5 0.0 0.0 0.01781389372950941 9.493710943928033E-4 32-33 7.911425786606695E-5 0.0 0.0 0.047850940299326156 0.0010021139329701814 34-35 7.911425786606695E-5 0.0 0.0 0.09233952463934447 0.0010284853522588703 36-37 1.318570964434449E-4 0.0 0.0 0.16699701264562297 0.0010284853522588703 38-39 1.8459993502082287E-4 0.0 0.0 0.2851277853493053 0.0010548567715475592 40-41 1.8459993502082287E-4 0.0 0.0 0.4166816104709302 0.0011075996101249372 42-43 2.1097135430951186E-4 0.0 0.0 0.5518878771640386 0.0011207853197692818 44-45 2.1097135430951186E-4 0.0 0.0 0.7107888640880342 0.0011339710294136262 46-47 2.1097135430951186E-4 0.0 0.0 0.9005839687087287 0.0011471567390579707 48-49 2.1097135430951186E-4 0.0 0.0 1.103604340102701 0.0011603424487023153 50-51 2.1097135430951186E-4 0.0 0.0 1.324082591065785 0.0011603424487023153 52-53 2.1097135430951186E-4 2.6371419288688983E-5 0.0 1.5386668298178474 0.0011867138679910042 54-55 2.1097135430951186E-4 5.2742838577377965E-5 0.0 1.7714341621694607 0.0011867138679910042 56-57 2.2415706395385635E-4 5.2742838577377965E-5 0.0 2.031601399162022 0.0012130852872796931 58-59 2.3734277359820083E-4 5.2742838577377965E-5 0.0 2.3162808703834195 0.0012262709969240377 60-61 2.3734277359820083E-4 5.2742838577377965E-5 0.0 2.625578061510808 0.0012394567065683823 62-63 2.5052848324254534E-4 5.2742838577377965E-5 0.0 2.9267660412069247 0.001318570964434449 64-65 2.900856121755788E-4 5.2742838577377965E-5 0.0 3.2328063620521608 0.0013449423837231381 66-67 3.164570314642678E-4 5.2742838577377965E-5 0.0 3.570650614559555 0.0013581280933674827 68-69 3.4282845075295676E-4 5.2742838577377965E-5 0.0 3.9460213967147544 0.001371313803011827 70-71 3.4282845075295676E-4 5.2742838577377965E-5 0.0 4.344335313651113 0.001371313803011827 72-73 3.4282845075295676E-4 5.2742838577377965E-5 0.0 4.7448380583884315 0.0014899851898109275 74-75 3.4282845075295676E-4 5.2742838577377965E-5 0.0 5.130704665420529 0.001529542318743961 76-77 3.4282845075295676E-4 5.2742838577377965E-5 0.0 5.541360406583994 0.0015559137380326499 78-79 3.4282845075295676E-4 7.911425786606695E-5 0.0 5.992469904936307 0.0015559137380326499 80-81 3.4282845075295676E-4 7.911425786606695E-5 0.0 6.453587356908679 0.0015822851573213388 82-83 3.4282845075295676E-4 7.911425786606695E-5 0.0 6.899857699821518 0.0017800708019865062 84-85 3.4282845075295676E-4 7.911425786606695E-5 0.0 7.327575749264764 0.0017932565116308508 86-87 3.4282845075295676E-4 7.911425786606695E-5 0.0 7.759209954472382 0.0018591850598525732 88-89 3.4282845075295676E-4 7.911425786606695E-5 0.0 8.217940792999126 0.0018987421887856066 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4525 0.0 53.15691 1 GTATCAA 8820 0.0 44.538143 1 ATCAACG 11245 0.0 35.69332 3 TCAACGC 11455 0.0 35.122734 4 TATCAAC 11635 0.0 34.53722 2 CAACGCA 11760 0.0 34.131577 5 AACGCAG 12250 0.0 32.882504 6 ACGCAGA 13990 0.0 28.45362 7 CGCAGAG 14230 0.0 27.807018 8 TACATGG 10790 0.0 24.886587 2 GTACATG 10855 0.0 24.781271 1 GCAGAGT 15625 0.0 23.927618 9 ACATGGG 11255 0.0 22.678423 3 AGAGTAC 14010 0.0 21.114187 10-11 GAGTACT 9845 0.0 20.939133 12-13 CAGAGTA 14640 0.0 20.578262 10-11 TGGTATC 2270 0.0 20.272915 2 GTGGTAT 2485 0.0 19.855337 1 AGTACTT 10430 0.0 19.83293 12-13 GTACTTT 10555 0.0 19.46526 14-15 >>END_MODULE