FastQCFastQC Report
Fri 27 May 2016
SRR939883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791984
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90720.23924151578698644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73580.19404090312617353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48590.12813872632373977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.053.9649241
GTATCAA93000.043.1537741
ATCAACG116750.033.883293
TATCAAC120100.033.1362152
TCAACGC119750.033.0729374
CAACGCA122250.032.3966035
AACGCAG127350.031.1152956
ACGCAGA145050.027.1559777
CGCAGAG147200.026.7273278
GCAGAGT164850.023.1511699
GTACATG115950.022.9393221
TACATGG112150.022.6617222
ACATGGG113500.021.10323
TGGTATC21800.020.9806372
GAGTACT100850.020.86066812-13
AGAGTAC150100.020.3723410-11
CAGAGTA158650.019.85355410-11
GTACTTT108550.019.29435714-15
GTGGTAT26550.019.0060311
AGTACTT107800.018.88198312-13
Produced by FastQC (version 0.11.5)