##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939883_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3791984 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41409088224001 31.0 31.0 34.0 30.0 34.0 2 31.639462877480497 31.0 31.0 34.0 30.0 34.0 3 31.698296459056788 31.0 31.0 34.0 30.0 34.0 4 35.50514849218773 37.0 35.0 37.0 33.0 37.0 5 35.23186121038486 37.0 35.0 37.0 33.0 37.0 6 35.24846755682513 37.0 35.0 37.0 32.0 37.0 7 35.165997008426196 37.0 35.0 37.0 32.0 37.0 8 35.101177378385565 37.0 35.0 37.0 32.0 37.0 9 36.720587428638936 39.0 35.0 39.0 32.0 39.0 10-11 36.57242752079122 38.5 35.0 39.0 32.0 39.0 12-13 36.51393584466601 38.0 35.0 39.0 32.0 39.0 14-15 37.57421352516255 39.0 36.0 41.0 32.0 41.0 16-17 37.49372439335187 39.0 36.0 41.0 32.0 41.0 18-19 37.45454635357112 39.0 36.0 41.0 32.0 41.0 20-21 37.36715964518838 39.0 36.0 41.0 31.5 41.0 22-23 37.22126662454272 39.0 36.0 40.0 31.0 41.0 24-25 37.06557780834518 39.0 36.0 40.0 31.0 41.0 26-27 36.76490591732455 39.0 36.0 40.0 30.0 41.0 28-29 36.53573841556293 38.0 35.0 40.0 30.0 41.0 30-31 36.287489873374994 38.0 35.0 40.0 30.0 41.0 32-33 36.25503601808447 38.0 35.0 40.0 30.0 41.0 34-35 36.35273975312132 38.0 35.0 40.0 30.0 41.0 36-37 36.32595522554948 38.0 35.0 40.0 30.0 41.0 38-39 36.19075278271217 38.0 35.0 40.0 30.0 41.0 40-41 36.01486148148304 38.0 35.0 40.0 29.0 41.0 42-43 35.81292444799345 38.0 35.0 40.0 28.5 41.0 44-45 35.578863993097016 38.0 34.0 40.0 27.5 41.0 46-47 35.29252773745881 38.0 34.0 40.0 27.0 41.0 48-49 34.99364831180722 38.0 33.5 40.0 26.0 41.0 50-51 34.72002756340744 38.0 33.0 40.0 26.0 41.0 52-53 34.42241778973751 38.0 33.0 40.0 24.5 41.0 54-55 34.09737409229575 37.0 33.0 40.0 24.0 41.0 56-57 32.767243348073194 36.5 31.5 39.5 16.0 41.0 58-59 29.33560175886818 34.5 24.0 38.5 7.5 40.0 60-61 29.29978159190545 34.0 24.0 38.0 9.0 40.0 62-63 31.18044656833995 35.0 29.0 38.0 11.5 40.0 64-65 30.710823278790205 35.0 29.0 38.0 4.5 40.0 66-67 25.23966609563753 28.5 18.0 36.0 2.0 39.0 68-69 17.544315192258196 13.0 7.0 29.0 2.0 35.5 70-71 16.36257602880181 14.0 7.5 26.0 2.0 33.5 72-73 18.489674798205897 18.5 7.0 30.0 2.0 34.5 74-75 19.208424666348805 18.0 7.5 30.5 2.0 35.0 76-77 20.208407920497557 23.0 8.5 31.0 2.0 34.5 78-79 23.287972865919265 28.0 12.0 34.0 2.0 36.0 80-81 25.95577130599707 31.5 22.0 34.5 2.0 36.0 82-83 26.944940300381013 32.5 26.0 35.0 2.0 36.0 84-85 26.942458881683045 33.0 26.0 35.0 2.0 36.0 86-87 26.655468219275186 33.0 25.0 35.0 2.0 35.5 88-89 26.216176808762906 33.0 24.5 35.0 2.0 35.0 90-91 24.575497417710622 31.5 11.5 34.5 2.0 35.0 92-93 22.450232384946773 30.5 2.0 34.0 2.0 35.0 94-95 18.026510924096726 23.0 2.0 33.0 2.0 35.0 96-97 16.69370176667412 14.0 2.0 32.5 2.0 34.5 98-99 15.598796962223469 2.0 2.0 32.0 2.0 34.0 100-101 14.954792003341787 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3570218680807571 1101 2 0.4252844981908055 1101 3 0.41108139583540293 1101 4 0.3550354349869451 1101 5 0.4711728952390217 1101 6 0.4527175092345246 1101 7 0.36785446204348915 1101 8 0.3734736145338857 1101 9 0.4832629062245246 1101 10-11 0.5034301988198138 1101 12-13 0.606016326846607 1101 14-15 0.6284691769673287 1101 16-17 0.6504832365349102 1101 18-19 0.6263679059876281 1101 20-21 0.6741447553571476 1101 22-23 0.6752431870736118 1101 24-25 0.4849338738094815 1101 26-27 0.6814926441877702 1101 28-29 0.6967704537857387 1101 30-31 0.7535020621697441 1101 32-33 0.6158625344271584 1101 34-35 0.8846524527755832 1101 36-37 0.9515636950552349 1101 38-39 0.8061115540079911 1101 40-41 0.8346444137092064 1101 42-43 0.7208861834392479 1101 44-45 0.7116878160607101 1101 46-47 0.650330172576659 1101 48-49 0.6573166241505319 1101 50-51 0.8324869000541071 1101 52-53 0.7231939053196115 1101 54-55 0.8310714146073224 1101 56-57 1.420770590996895 1101 58-59 -8.021152710980648 1101 60-61 -9.361591445178085 1101 62-63 -2.367071770150396 1101 64-65 0.13627033723176396 1101 66-67 -0.7753687189744021 1101 68-69 -3.42989634116252 1101 70-71 -4.002786017407567 1101 72-73 -6.510533615964299 1101 74-75 -7.249827412521103 1101 76-77 -8.258166902293539 1101 78-79 -11.406056999404907 1101 80-81 -7.327186782089168 1101 82-83 -3.4859172167109342 1101 84-85 -2.5331849802474657 1101 86-87 -2.431321193770774 1101 88-89 -2.5207678439051016 1101 90-91 -8.334137921238918 1101 92-93 -14.150209001062937 1101 94-95 -12.363947983277761 1101 96-97 -12.35404851874895 1101 98-99 -11.708938968692085 1101 100-101 -11.50139073689133 1102 1 0.4806645563488736 1102 2 0.4664998486407299 1102 3 0.5254424271356015 1102 4 0.3184660697461865 1102 5 0.42235115089824404 1102 6 0.5305772216461975 1102 7 0.47722333492909286 1102 8 0.5062759920084119 1102 9 0.5636043486950939 1102 10-11 0.6033004399191384 1102 12-13 0.6024318423117379 1102 14-15 0.6368605141173873 1102 16-17 0.7463604907805248 1102 18-19 0.7119207797969551 1102 20-21 0.8067711876095487 1102 22-23 0.7378347890103001 1102 24-25 0.7653451562758278 1102 26-27 0.7531872113535556 1102 28-29 0.7214735217009363 1102 30-31 0.8293193190385608 1102 32-33 0.6699104516146335 1102 34-35 0.757990726102193 1102 36-37 0.8084216911742388 1102 38-39 0.7077040292248853 1102 40-41 0.6799426573958627 1102 42-43 0.7211657115764751 1102 44-45 0.5952694195556276 1102 46-47 0.7007883364013381 1102 48-49 0.6606755580088048 1102 50-51 0.6078915068033623 1102 52-53 0.630994853484431 1102 54-55 0.434603154811505 1102 56-57 1.16993906905671 1102 58-59 -8.501286315004688 1102 60-61 -9.62104988813854 1102 62-63 -2.5934706159407703 1102 64-65 0.1888456730634971 1102 66-67 -1.1232671852970633 1102 68-69 -3.569202750411442 1102 70-71 -4.059162476284365 1102 72-73 -6.568732640894822 1102 74-75 -7.380929247754764 1102 76-77 -8.567442505714466 1102 78-79 -11.664885929530158 1102 80-81 -7.711425980151137 1102 82-83 -3.372256154806692 1102 84-85 -2.512736546939056 1102 86-87 -2.2452520308754274 1102 88-89 -2.46177395462448 1102 90-91 -8.296504084601732 1102 92-93 -14.318008839410812 1102 94-95 -12.637082397804479 1102 96-97 -12.418592221434594 1102 98-99 -11.909605531476895 1102 100-101 -11.561685578260375 1103 1 0.304194850698142 1103 2 0.28621494485639687 1103 3 0.2563615776795132 1103 4 0.1967773726865687 1103 5 0.17718517858478577 1103 6 0.1744374626887364 1103 7 0.26807754096813596 1103 8 0.3004982321973486 1103 9 0.4514536469694832 1103 10-11 0.3886172989808614 1103 12-13 0.3986566028512257 1103 14-15 0.5353383529906495 1103 16-17 0.5352898782078483 1103 18-19 0.5425037799469905 1103 20-21 0.5113938385798704 1103 22-23 0.418665159778584 1103 24-25 0.4240243899711018 1103 26-27 0.40397534564890947 1103 28-29 0.42035335726399836 1103 30-31 0.5102369147702888 1103 32-33 0.5253433540836028 1103 34-35 0.5644676849517509 1103 36-37 0.32398361195810566 1103 38-39 0.479015140145961 1103 40-41 0.6265162011782124 1103 42-43 0.6053649716413503 1103 44-45 0.45371795799329817 1103 46-47 0.4002523537717053 1103 48-49 0.46548498079255296 1103 50-51 0.42274241197614515 1103 52-53 0.399749308993087 1103 54-55 0.3466875826678333 1103 56-57 1.0709826589936569 1103 58-59 -6.457460901755045 1103 60-61 -8.530411684381956 1103 62-63 -2.3937965045742366 1103 64-65 -0.08416438112948299 1103 66-67 -1.716323471830293 1103 68-69 -4.028504569774917 1103 70-71 -4.750714948474608 1103 72-73 -7.685197169020988 1103 74-75 -8.299969050169233 1103 76-77 -9.138451244536338 1103 78-79 -12.146526707278309 1103 80-81 -8.467372670305831 1103 82-83 -4.6472958996931055 1103 84-85 -3.7058903201961293 1103 86-87 -3.4384793856190434 1103 88-89 -3.589581342936487 1103 90-91 -9.097128069600902 1103 92-93 -14.857086322698525 1103 94-95 -12.669631185867253 1103 96-97 -12.629374221995882 1103 98-99 -12.140737380327383 1103 100-101 -11.724053176285452 1104 1 0.14712600332129 1104 2 0.13870764829720628 1104 3 0.15796610110646014 1104 4 0.1666053575196429 1104 5 0.051107818400083715 1104 6 0.15288086428319758 1104 7 0.13005757034360244 1104 8 0.18522359080214557 1104 9 0.15144693075026794 1104 10-11 0.1848145929841536 1104 12-13 0.14544194582215653 1104 14-15 0.17002829819749365 1104 16-17 0.2078270845964667 1104 18-19 0.2547984388736566 1104 20-21 0.19991554675960543 1104 22-23 0.24239155673219415 1104 24-25 0.2457303243104576 1104 26-27 0.2408267874106187 1104 28-29 0.3126657781918496 1104 30-31 0.2786321927884501 1104 32-33 0.2603465296293095 1104 34-35 0.21673500193678308 1104 36-37 0.17367928993778747 1104 38-39 0.15618737065832988 1104 40-41 0.1483857900576382 1104 42-43 0.15975971769231023 1104 44-45 0.0807748667574586 1104 46-47 0.06215644589477165 1104 48-49 0.09385541679511533 1104 50-51 0.021452911212442416 1104 52-53 4.547518757718194E-4 1104 54-55 -0.1240030165708319 1104 56-57 0.43687761785814416 1104 58-59 -6.805427486432311 1104 60-61 -8.743107584103473 1104 62-63 -2.664200018755988 1104 64-65 -0.5093511959651877 1104 66-67 -1.9604748642064749 1104 68-69 -4.34423889906315 1104 70-71 -4.941532197751082 1104 72-73 -7.832330043021782 1104 74-75 -8.689295585285066 1104 76-77 -9.30748245789863 1104 78-79 -12.251049901021776 1104 80-81 -8.735873788742897 1104 82-83 -4.961926017245602 1104 84-85 -3.99049893785163 1104 86-87 -3.876519331665417 1104 88-89 -3.9839658307621413 1104 90-91 -9.54676452092124 1104 92-93 -15.094489047458 1104 94-95 -12.664869466397867 1104 96-97 -12.396881899673861 1104 98-99 -11.776200856754386 1104 100-101 -11.291960715534692 1105 1 0.12029484051095807 1105 2 0.193304913623777 1105 3 0.1755729109257267 1105 4 0.13088450266998564 1105 5 0.16555426713510712 1105 6 0.1346021778966744 1105 7 0.1698960053404477 1105 8 0.19649712026374289 1105 9 0.22509613884484025 1105 10-11 0.16123387004657985 1105 12-13 0.3046221031302849 1105 14-15 0.2699786099874757 1105 16-17 0.24892604447184397 1105 18-19 0.2816141056314905 1105 20-21 0.26500417745715055 1105 22-23 0.3450708544631951 1105 24-25 0.2506693969244864 1105 26-27 0.24139217483613606 1105 28-29 0.26857718944457076 1105 30-31 0.22288105979507122 1105 32-33 0.43161602810040733 1105 34-35 0.3493225549695822 1105 36-37 0.26275059974616255 1105 38-39 0.4306941592066593 1105 40-41 0.22432401048963158 1105 42-43 0.1873888431717461 1105 44-45 0.25121480540250474 1105 46-47 0.22104069918941605 1105 48-49 0.2852091774887384 1105 50-51 0.2940386934598962 1105 52-53 0.24288252682549683 1105 54-55 0.2973448361267188 1105 56-57 1.0090459277114263 1105 58-59 -6.174599458059944 1105 60-61 -8.228923586732357 1105 62-63 -2.2181935094727443 1105 64-65 0.1267388010740973 1105 66-67 -1.4046140823556712 1105 68-69 -3.9605739165921783 1105 70-71 -4.420099778960479 1105 72-73 -5.840637641479194 1105 74-75 -6.519533035902743 1105 76-77 -8.530079823529366 1105 78-79 -11.977906227270086 1105 80-81 -7.989726961191572 1105 82-83 -3.9508903068110897 1105 84-85 -3.0720736877164363 1105 86-87 -2.834985070531989 1105 88-89 -2.949212016985296 1105 90-91 -8.390749423934103 1105 92-93 -11.317982562242378 1105 94-95 -11.623163773880961 1105 96-97 -11.920781854738669 1105 98-99 -11.349611539927945 1105 100-101 -10.930094107437053 1106 1 0.14198656053029524 1106 2 0.1814580501877927 1106 3 0.0921819901360088 1106 4 0.10898392277835 1106 5 0.061452649714141216 1106 6 0.05086011408022273 1106 7 0.05375376376134966 1106 8 0.09239578375719759 1106 9 0.12983510895077188 1106 10-11 0.19951035613303247 1106 12-13 0.139437505836554 1106 14-15 0.23167701717461853 1106 16-17 0.08515342840291851 1106 18-19 0.11992752494149528 1106 20-21 0.15989527114969349 1106 22-23 0.20027980141896506 1106 24-25 0.21649717587742856 1106 26-27 0.2211460404292609 1106 28-29 0.2377646697630027 1106 30-31 0.21236808337474855 1106 32-33 0.22540637267847075 1106 34-35 0.16418260480941882 1106 36-37 0.12696106307797805 1106 38-39 0.22429309572084577 1106 40-41 0.2724097121422133 1106 42-43 0.3126984108641153 1106 44-45 0.3068179174286527 1106 46-47 0.06545319195937793 1106 48-49 0.07475845170419149 1106 50-51 0.24588529143174753 1106 52-53 0.2681337485925397 1106 54-55 0.02268799416102496 1106 56-57 0.8083572238702317 1106 58-59 -6.397356377473745 1106 60-61 -8.762064009109661 1106 62-63 -2.396650751348208 1106 64-65 -0.018387382874848868 1106 66-67 -1.3338148695902419 1106 68-69 -3.891361448935962 1106 70-71 -4.410569054928841 1106 72-73 -5.857152530083987 1106 74-75 -6.5147738540041935 1106 76-77 -8.441098916439845 1106 78-79 -11.934477279095145 1106 80-81 -7.962414810772014 1106 82-83 -3.8977090633546396 1106 84-85 -2.8231654265008572 1106 86-87 -2.7058146150320646 1106 88-89 -2.756937648150842 1106 90-91 -8.27642174905996 1106 92-93 -11.613186959789095 1106 94-95 -11.612068286293585 1106 96-97 -12.004959792394208 1106 98-99 -11.601484396375339 1106 100-101 -11.329686268857222 1107 1 0.07834322749760148 1107 2 0.12727971502354052 1107 3 0.06647707656645352 1107 4 0.07862430803903919 1107 5 0.09946963721664304 1107 6 0.09993880928496424 1107 7 0.10243149587038403 1107 8 0.1301903718898103 1107 9 0.1113179805579918 1107 10-11 0.2166010754689438 1107 12-13 0.15467347324860725 1107 14-15 0.17107367905531135 1107 16-17 0.24333329963536698 1107 18-19 0.26471028501290306 1107 20-21 0.15576561735884553 1107 22-23 0.18306198672525653 1107 24-25 0.3194673138602653 1107 26-27 0.3888725586690853 1107 28-29 0.31660678693778266 1107 30-31 0.3562395382195547 1107 32-33 0.24157780393885986 1107 34-35 0.20457231909065854 1107 36-37 0.20848527743959977 1107 38-39 0.13646684228751838 1107 40-41 0.007618403419961339 1107 42-43 0.19208715711987878 1107 44-45 0.2920106430085525 1107 46-47 0.16455879076548285 1107 48-49 0.14283065531221695 1107 50-51 0.21899282404798726 1107 52-53 0.21669850238187394 1107 54-55 0.08075481704877774 1107 56-57 0.8222109679944367 1107 58-59 -6.6067911805662725 1107 60-61 -8.878180778862454 1107 62-63 -2.6929411214554904 1107 64-65 -0.34052523743238083 1107 66-67 -1.881188876425803 1107 68-69 -4.278717148438 1107 70-71 -4.7307996388196205 1107 72-73 -6.053435099407327 1107 74-75 -6.730947862184234 1107 76-77 -9.120758533363933 1107 78-79 -12.488804767489203 1107 80-81 -8.42460796826606 1107 82-83 -4.275966135738631 1107 84-85 -3.358020995756412 1107 86-87 -3.113973688842325 1107 88-89 -3.264424790162085 1107 90-91 -8.507514441420009 1107 92-93 -11.683023723133196 1107 94-95 -11.944313477957587 1107 96-97 -12.398359979052824 1107 98-99 -11.699533248049525 1107 100-101 -11.182144244059412 1108 1 -0.05309715308267471 1108 2 -0.038922854565178966 1108 3 -0.09711313811720501 1108 4 -0.0797600604685087 1108 5 -0.17944017394029288 1108 6 -0.08643854608522616 1108 7 -0.05900826453562047 1108 8 -0.05284863707170473 1108 9 0.001025800955524403 1108 10-11 -0.01200000518471711 1108 12-13 -0.08997048211952574 1108 14-15 0.005855248509661237 1108 16-17 -0.004973366204403362 1108 18-19 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5.7747457331271015 2312 78-79 5.915114207530305 2312 80-81 3.5370968506956935 2312 82-83 1.955949518125923 2312 84-85 1.6150332737173265 2312 86-87 1.397607804240831 2312 88-89 1.6158574349630364 2312 90-91 3.148825580079844 2312 92-93 5.058062116791518 2312 94-95 9.146933610857744 2312 96-97 9.826247794738542 2312 98-99 10.485286225021484 2312 100-101 9.585196981480854 2313 1 -0.305120731108925 2313 2 -0.25156262121564055 2313 3 -0.28429849435336507 2313 4 -0.3238837843382214 2313 5 -0.32942599471488165 2313 6 -0.35554403952227176 2313 7 -0.3391256145059103 2313 8 -0.3865722367043958 2313 9 -0.36421416555450037 2313 10-11 -0.39936800334817235 2313 12-13 -0.4058738338183261 2313 14-15 -0.6199759241797977 2313 16-17 -0.5761827866116818 2313 18-19 -0.5231852031509518 2313 20-21 -0.5680156611859317 2313 22-23 -0.508122695192398 2313 24-25 -0.45797579262509913 2313 26-27 -0.6341660896630827 2313 28-29 -0.6014136314600265 2313 30-31 -0.5209255085194613 2313 32-33 -0.4620912486902711 2313 34-35 -0.6430393344166703 2313 36-37 -0.8140452223636245 2313 38-39 -0.88488949435731 2313 40-41 -1.0548782383705415 2313 42-43 -0.7498564196358402 2313 44-45 -0.7075442580894489 2313 46-47 -0.5735586538682327 2313 48-49 -0.6558855889340549 2313 50-51 -0.4718509542245073 2313 52-53 -0.655075861760821 2313 54-55 -0.5798458946254001 2313 56-57 0.6143426407869583 2313 58-59 4.068234220809348 2313 60-61 3.775418096264435 2313 62-63 1.1251935002685265 2313 64-65 1.0814665813641966 2313 66-67 1.6912517564811154 2313 68-69 0.026104925756765596 2313 70-71 2.699292725858454 2313 72-73 8.378368802456958 2313 74-75 10.003375834358518 2313 76-77 8.815909629067395 2313 78-79 6.500002774069092 2313 80-81 3.438954817302765 2313 82-83 1.997978563566683 2313 84-85 1.550378485227771 2313 86-87 1.450208713607438 2313 88-89 1.4117302402784233 2313 90-91 2.857054838865473 2313 92-93 4.827196246244775 2313 94-95 8.785003582037099 2313 96-97 9.573516619878454 2313 98-99 9.96977036942585 2313 100-101 9.205655556311674 2314 1 -0.37430255491665676 2314 2 -0.3308354535053688 2314 3 -0.3021090336844168 2314 4 -0.2473095138781929 2314 5 -0.42782926749466554 2314 6 -0.29539555298600817 2314 7 -0.33603995876539017 2314 8 -0.3804013940492439 2314 9 -0.39585366219802154 2314 10-11 -0.367833054878389 2314 12-13 -0.5118121544589798 2314 14-15 -0.5896367161455061 2314 16-17 -0.5941867166228576 2314 18-19 -0.6569454493408031 2314 20-21 -0.5181091450686353 2314 22-23 -0.6158769347849287 2314 24-25 -0.5609517587346176 2314 26-27 -0.743759142538515 2314 28-29 -0.6640721336800723 2314 30-31 -0.684655918629602 2314 32-33 -0.6098187558304886 2314 34-35 -0.7900386768102265 2314 36-37 -0.7657116171112719 2314 38-39 -0.6253577170258566 2314 40-41 -0.6995365768140758 2314 42-43 -0.8433050622598657 2314 44-45 -0.8251234474707374 2314 46-47 -0.6877290883323042 2314 48-49 -0.7509809043632316 2314 50-51 -0.6008040941390291 2314 52-53 -0.5584258415387993 2314 54-55 -0.45124398344102445 2314 56-57 0.800069580335304 2314 58-59 3.892114930249189 2314 60-61 3.7332371275951353 2314 62-63 1.1589630292110265 2314 64-65 1.1173827859248107 2314 66-67 1.9373758165633426 2314 68-69 -0.26031612615101096 2314 70-71 2.7507390224777186 2314 72-73 8.483214524358193 2314 74-75 10.064572850000424 2314 76-77 9.0061465174137 2314 78-79 6.612140301331319 2314 80-81 3.636226594421686 2314 82-83 2.026248921080807 2314 84-85 1.585853368349479 2314 86-87 1.5673855722364287 2314 88-89 1.5723059991043833 2314 90-91 2.748191904177922 2314 92-93 4.880907909570276 2314 94-95 8.78537442331703 2314 96-97 9.367536401830659 2314 98-99 9.79025381018282 2314 100-101 9.080970763585851 2315 1 -0.7281542942823513 2315 2 -0.5252066461883373 2315 3 -0.4928733771240523 2315 4 -0.3484835202666332 2315 5 -0.6450640862271015 2315 6 -0.6626364808804439 2315 7 -0.6287155971728211 2315 8 -0.7001563293793964 2315 9 -1.0780787935450036 2315 10-11 -0.9021997093267231 2315 12-13 -0.7219847819222807 2315 14-15 -0.7483845124834971 2315 16-17 -0.910444418530659 2315 18-19 -0.745342928875111 2315 20-21 -0.8071611776741108 2315 22-23 -0.8658586072989394 2315 24-25 -0.8889641865156008 2315 26-27 -0.9236872289744369 2315 28-29 -0.848633408574905 2315 30-31 -0.791110684648686 2315 32-33 -0.7189535239441582 2315 34-35 -0.8796202864871319 2315 36-37 -0.905073471503762 2315 38-39 -0.830788587494169 2315 40-41 -0.8324384287629059 2315 42-43 -0.8710979093038986 2315 44-45 -0.8578008313530248 2315 46-47 -0.8894536174904673 2315 48-49 -0.7388226247032605 2315 50-51 -0.7142672908016792 2315 52-53 -0.743298160053051 2315 54-55 -0.5442086724846789 2315 56-57 0.7350488964582524 2315 58-59 4.302473276237219 2315 60-61 3.9383803438071006 2315 62-63 1.389357681076067 2315 64-65 1.1449099716957 2315 66-67 1.9619346477933775 2315 68-69 6.0906007717910775 2315 70-71 4.968668191108042 2315 72-73 9.119862165348923 2315 74-75 10.166972179738082 2315 76-77 9.046725316876092 2315 78-79 6.495675586105545 2315 80-81 3.4407445815400806 2315 82-83 1.7278453323845042 2315 84-85 1.1630469938753016 2315 86-87 1.164070217294114 2315 88-89 1.150606604784155 2315 90-91 2.539732285738726 2315 92-93 4.455894905782593 2315 94-95 8.57831327933281 2315 96-97 9.29707332735125 2315 98-99 9.382351696412767 2315 100-101 8.567528861716445 2316 1 -0.43963636876894796 2316 2 -0.4557071682906688 2316 3 -0.5624758832152459 2316 4 -0.48414241340968545 2316 5 -0.5748225268814693 2316 6 -0.5231836441244369 2316 7 -0.6080180684571914 2316 8 -0.6760839311893534 2316 9 -0.7523565519856561 2316 10-11 -0.639632358125894 2316 12-13 -0.634387148786189 2316 14-15 -0.7796055358040661 2316 16-17 -1.023345915224013 2316 18-19 -0.9818009866544273 2316 20-21 -0.8844859253246469 2316 22-23 -0.9400625752766629 2316 24-25 -2.2062715307550675 2316 26-27 -1.5926089136246233 2316 28-29 -1.1464336174158376 2316 30-31 -0.8443748684287158 2316 32-33 -0.6803485813754122 2316 34-35 -0.8217079996789209 2316 36-37 -0.7307079998713206 2316 38-39 -1.127388657107815 2316 40-41 -0.929452699559981 2316 42-43 -0.9628793224608785 2316 44-45 -0.9201224463895628 2316 46-47 -0.8809920790289283 2316 48-49 -1.0995925516089287 2316 50-51 -1.1254291425246166 2316 52-53 -0.9588672515949881 2316 54-55 -0.8960297654188949 2316 56-57 0.31259014254248285 2316 58-59 3.7382790541696913 2316 60-61 3.6571920388993426 2316 62-63 1.1217878933976841 2316 64-65 0.7491668460430745 2316 66-67 5.963893575743256 2316 68-69 8.726975989333713 2316 70-71 5.499132514115354 2316 72-73 9.279841281255639 2316 74-75 10.149282656591541 2316 76-77 8.698693294599725 2316 78-79 6.121024002395135 2316 80-81 3.0226944597162024 2316 82-83 1.8442818099341025 2316 84-85 1.4732767189014062 2316 86-87 1.2881384386655057 2316 88-89 1.4159139490236292 2316 90-91 2.7866832081195128 2316 92-93 4.66028056537187 2316 94-95 8.872185886363788 2316 96-97 9.451422091709063 2316 98-99 9.654172789346985 2316 100-101 8.619736310376384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 31.0 4 66.0 5 146.0 6 334.0 7 900.0 8 2220.0 9 3754.0 10 6134.0 11 9581.0 12 13224.0 13 17865.0 14 23769.0 15 32288.0 16 43804.0 17 55682.0 18 62252.0 19 65458.0 20 69134.0 21 68550.0 22 66287.0 23 63921.0 24 65777.0 25 70608.0 26 92967.0 27 134714.0 28 188318.0 29 247979.0 30 292196.0 31 310248.0 32 320889.0 33 330122.0 34 333397.0 35 320490.0 36 265871.0 37 161889.0 38 49027.0 39 2089.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94367593285467 19.213349514499086 14.621974504904598 24.221000047741647 2 16.003298686749236 20.162972214867192 39.394094432951846 24.439634665431726 3 19.46226049860806 23.66017412145462 29.68828293206774 27.18928244786958 4 13.477957712901741 16.738440879497382 36.27214671791864 33.51145468968223 5 13.970789833466638 37.034191996930524 34.356817697834394 14.638200471768439 6 33.27526343743193 36.02970689206858 16.708649988317458 13.986379682182035 7 29.142791741737305 31.935076730281565 20.287532858788435 18.634598669192698 8 26.268928775430663 35.80012858724391 19.05696710265724 18.873975534668187 9 26.021866126017407 16.519531727981978 19.549871518445226 37.90873062755539 10-11 25.29655979561095 26.133074401157813 27.272833957105302 21.297531846125935 12-13 26.026256967328976 23.188679066156396 27.584438647420452 23.200625319094172 14-15 22.640895923746324 24.85623255114189 25.51447801138958 26.988393513722205 16-17 22.659998037966407 27.95446921716969 26.30173281321862 23.08379993164528 18-19 22.575343159367115 26.692649014018837 28.238364412245097 22.49364341436895 20-21 23.091065257659317 26.006241060088865 28.399223203473433 22.503470478778393 22-23 22.86561599416031 25.85223197144292 28.327848957168595 22.954303077228175 24-25 22.686857328511934 26.11027630918274 28.389624006852348 22.813242355452974 26-27 22.551189952371843 26.369775996943023 28.18716451394318 22.891869536741957 28-29 22.579015200043674 26.568139904644035 28.124142085083008 22.728702810229287 30-31 22.761396311483246 26.298263346988403 28.00372926437359 22.93661107715476 32-33 22.472197531919058 26.27351528867085 28.094374052357157 23.15991312705294 34-35 22.947078998650955 26.45960403692617 27.897125076593287 22.69619188782958 36-37 23.070386404777057 26.11328693002905 27.952216246871735 22.864110418322163 38-39 22.715960870724118 26.352159526276786 27.972992881042035 22.958886721957057 40-41 23.071001158928585 26.20550268840569 27.91602693905555 22.807469213610176 42-43 22.766612529375656 26.216987749074843 27.878590200578795 23.137809520970702 44-45 22.816096380762254 26.35581609321534 27.993275589005854 22.834811937016546 46-47 22.97486191940599 26.582947805034262 27.489565807820938 22.952624467738815 48-49 22.836548797527975 26.645434236047965 27.42637036755661 23.091646598867445 50-51 22.869573566526828 26.782952157067974 27.363513539178363 22.983960737226834 52-53 23.081086570329408 26.512471661309956 27.341326415549716 23.06511535281092 54-55 22.788050980240627 26.60966998783164 27.477702441887747 23.124576590039986 56-57 22.716566399186945 26.613620600675187 27.599105806709932 23.070707193427932 58-59 21.262844382214276 26.30780553190189 29.218530184215176 23.210819901668657 60-61 22.63049589982731 26.454925477676767 27.8038037974491 23.110774825046825 62-63 22.592658667918787 26.580547807820658 27.62454382202806 23.202249702232493 64-65 21.791350484722454 26.5557313631846 28.067938124224 23.584980027868948 66-67 21.825673432357245 26.19119856284511 28.77597806560395 23.207149939193695 68-69 22.5180803617996 25.359663256732873 29.434392799771764 22.687863581695762 70-71 21.70113271578237 25.666972019394883 29.412474939111373 23.219420325711372 72-73 21.557520317423865 25.928061244702832 29.28681024335248 23.227608194520826 74-75 22.097918727276937 26.04785003171337 28.58021982848135 23.274011412528342 76-77 20.568719671479414 26.325802470090437 29.186345736402274 23.919132122027875 78-79 21.656425286246947 26.522414860705705 27.896696769215513 23.92446308383183 80-81 22.286568646492743 26.597225271425216 27.06170624301403 24.05449983906801 82-83 22.472164937578786 26.666517228466397 26.863076809476844 23.998241024477977 84-85 22.443876989954862 26.53830462847539 26.86657614235085 24.151242239218902 86-87 22.35289525772875 26.51324961638265 26.884570901729216 24.249284224159382 88-89 22.33071719994525 26.54675667058634 26.697258400156333 24.42526772931208 90-91 21.999550445451472 26.514137909116776 27.010088284214678 24.47622336121708 92-93 21.84076743550221 26.369368619728206 27.21725382667718 24.5726101180924 94-95 20.465657685596533 26.164514773437286 28.606735208368583 24.7630923325976 96-97 20.324751845017094 25.911888543759154 28.784976759479676 24.978382851744076 98-99 20.553288579701345 26.072219658974927 28.101725631341125 25.272766129982603 100-101 20.50471895798249 26.113050078483834 28.003545473101717 25.378685490431963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 217.0 1 157.0 2 114.5 3 144.0 4 173.0 5 199.5 6 266.5 7 419.5 8 648.5 9 915.5 10 1126.0 11 1280.5 12 1505.5 13 1808.5 14 2123.0 15 2407.0 16 2695.5 17 3078.0 18 3566.0 19 4230.5 20 5048.5 21 6030.5 22 7452.0 23 9313.5 24 11515.5 25 14584.0 26 18617.0 27 23253.0 28 28020.5 29 33257.5 30 39152.0 31 46288.5 32 54437.5 33 61926.5 34 68308.0 35 74637.0 36 82356.5 37 90603.0 38 97353.5 39 103329.0 40 109541.5 41 116189.5 42 122846.5 43 128690.0 44 134447.5 45 139364.0 46 143454.5 47 146951.5 48 149281.5 49 150375.0 50 149987.5 51 148822.0 52 146714.5 53 144002.0 54 139664.5 55 131685.5 56 120898.5 57 108412.0 58 95389.0 59 81916.0 60 67717.5 61 54218.0 62 42422.0 63 31956.0 64 23440.0 65 17256.0 66 12475.0 67 8885.0 68 6362.0 69 4519.5 70 3273.0 71 2434.5 72 1843.5 73 1414.0 74 989.0 75 651.5 76 423.0 77 244.5 78 151.0 79 92.5 80 53.5 81 34.0 82 19.5 83 10.0 84 7.0 85 6.0 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01964670737007329 2 0.004535884117654505 3 0.003902970054725969 4 0.0 5 0.06363423474360651 6 5.274283857737796E-5 7 0.0 8 1.5822851573213387E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5052848324254534E-4 16-17 0.0 18-19 0.0013317566740787936 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1867138679910042E-4 28-29 0.0022943134781159415 30-31 0.009269553879974177 32-33 2.5052848324254534E-4 34-35 0.0010416710619032148 36-37 0.02496054835674412 38-39 0.02398480584306263 40-41 0.025909919451136923 42-43 0.026503276385132425 44-45 0.02689884767446276 46-47 0.028533875670361482 48-49 0.02861298992822755 50-51 0.02405073439128435 52-53 0.020807049818775604 54-55 0.01565143734783691 56-57 0.15237406065004494 58-59 0.7431202241359668 60-61 0.3025461077894843 62-63 4.0875699897467927E-4 64-65 0.140625593356934 66-67 1.3884947826784078 68-69 2.6661768615057446 70-71 2.1498244718332145 72-73 1.8166347748302736 74-75 1.5230681353085878 76-77 1.0969191853130182 78-79 0.40594844282043385 80-81 2.5052848324254534E-4 82-83 1.0548567715475592E-4 84-85 2.637141928868898E-5 86-87 0.0031513846049983337 88-89 2.637141928868898E-5 90-91 0.21726093780986416 92-93 0.16848699783543392 94-95 1.2564794577192309 96-97 1.2313738665563991 98-99 0.9752546424246515 100-101 0.38958497715180235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3791984.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.938352073636636 #Duplication Level Percentage of deduplicated Percentage of total 1 69.86508158954199 33.492168788928126 2 15.088145472056397 14.466016595553718 3 6.044518462698784 8.692927625413537 4 2.957636015135987 5.671367863970266 5 1.645999051916279 3.9453241031817345 6 1.0308388006680549 2.9650028014554333 7 0.6752013355051738 2.2657627539425675 8 0.4755675531297583 1.8238339837385802 9 0.3484504745775859 1.5033727377473474 >10 1.702212660192674 14.605096553575986 >50 0.10984301728741584 3.6374173103009597 >100 0.0523077592574 4.343072549503373 >500 0.0024303099315008325 0.803752471430726 >1k 0.001657029469660181 1.349843409085548 >5k 1.104686313106785E-4 0.435040452171983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9072 0.23924151578698644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7358 0.19404090312617353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4859 0.12813872632373977 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2742838577377965E-5 2 0.0 0.0 0.0 0.0 5.2742838577377965E-5 3 0.0 0.0 0.0 2.6371419288688983E-5 5.2742838577377965E-5 4 0.0 0.0 0.0 2.6371419288688983E-5 5.2742838577377965E-5 5 0.0 0.0 0.0 2.6371419288688983E-5 5.2742838577377965E-5 6 2.6371419288688983E-5 0.0 0.0 2.6371419288688983E-5 5.2742838577377965E-5 7 2.6371419288688983E-5 0.0 0.0 2.6371419288688983E-5 5.2742838577377965E-5 8 2.6371419288688983E-5 0.0 0.0 2.6371419288688983E-5 1.0548567715475593E-4 9 2.6371419288688983E-5 0.0 0.0 5.2742838577377965E-5 1.0548567715475593E-4 10-11 2.6371419288688983E-5 0.0 0.0 5.2742838577377965E-5 1.0548567715475593E-4 12-13 2.6371419288688983E-5 0.0 0.0 6.592854822172245E-5 2.900856121755788E-4 14-15 2.6371419288688983E-5 0.0 0.0 7.911425786606695E-5 3.691998700416458E-4 16-17 7.911425786606695E-5 0.0 0.0 1.318570964434449E-4 5.537998050624686E-4 18-19 7.911425786606695E-5 0.0 0.0 1.582285157321339E-4 6.592854822172245E-4 20-21 7.911425786606695E-5 0.0 0.0 2.1097135430951186E-4 6.856569015059135E-4 22-23 7.911425786606695E-5 0.0 0.0 3.4282845075295676E-4 7.25214030438947E-4 24-25 7.911425786606695E-5 0.0 0.0 6.329140629285356E-4 7.515854497276359E-4 26-27 7.911425786606695E-5 0.0 0.0 0.001371313803011827 8.306997075937029E-4 28-29 1.1867138679910042E-4 0.0 0.0 0.004760041181608362 8.834425461710809E-4 30-31 1.450428060877894E-4 0.0 0.0 0.018367693534571877 8.966282558154254E-4 32-33 1.582285157321339E-4 0.0 0.0 0.05001339668099865 9.757425136814923E-4 34-35 1.582285157321339E-4 0.0 0.0 0.09702045156308675 0.0010548567715475592 36-37 1.582285157321339E-4 0.0 0.0 0.1761610808484424 0.0010548567715475592 38-39 1.582285157321339E-4 0.0 0.0 0.3007792226971422 0.0010680424811919038 40-41 1.582285157321339E-4 0.0 0.0 0.439980759412487 0.0010812281908362483 42-43 1.845999350208229E-4 0.0 0.0 0.581872180895278 0.0011075996101249372 44-45 2.3734277359820083E-4 0.0 0.0 0.7487637078637462 0.0011998995776353488 46-47 2.900856121755788E-4 0.0 0.0 0.940207026189984 0.0013185709644344492 48-49 2.900856121755788E-4 0.0 0.0 1.1281561314604702 0.0013449423837231381 50-51 2.900856121755788E-4 1.3185709644344491E-5 0.0 1.3221179203287776 0.001371313803011827 52-53 3.032713218199233E-4 3.9557128933033474E-5 0.0 1.5068365267363997 0.0013844995126561714 54-55 3.164570314642678E-4 5.2742838577377965E-5 0.0 1.7084328414887826 0.001397685222300516 56-57 3.164570314642678E-4 5.2742838577377965E-5 0.0 1.8833940227595898 0.0014108709319448605 58-59 3.164570314642678E-4 5.2742838577377965E-5 0.0 2.0067858936113656 0.001424056641589205 60-61 3.164570314642678E-4 5.2742838577377965E-5 0.0 2.10304157401508 0.001424056641589205 62-63 3.4282845075295676E-4 5.2742838577377965E-5 0.0 2.1859533162587184 0.0014372423512335497 64-65 3.4282845075295676E-4 5.2742838577377965E-5 0.0 2.2744426136819142 0.001450428060877894 66-67 3.4282845075295676E-4 5.2742838577377965E-5 0.0 2.3494561158485903 0.001450428060877894 68-69 3.4282845075295676E-4 5.2742838577377965E-5 0.0 2.4337523576048845 0.001450428060877894 70-71 3.4282845075295676E-4 5.2742838577377965E-5 0.0 2.5857176612559547 0.001450428060877894 72-73 3.4282845075295676E-4 6.592854822172245E-5 0.0 2.7796135215760405 0.001450428060877894 74-75 3.4282845075295676E-4 7.911425786606695E-5 0.0 2.9964393309676414 0.001450428060877894 76-77 3.4282845075295676E-4 7.911425786606695E-5 0.0 3.308769235313229 0.001450428060877894 78-79 3.4282845075295676E-4 7.911425786606695E-5 0.0 3.747826995050612 0.001450428060877894 80-81 3.8238557968599024E-4 7.911425786606695E-5 0.0 4.2365421373085965 0.0014899851898109275 82-83 3.9557128933033475E-4 7.911425786606695E-5 0.0 4.706362157646234 0.001582285157321339 84-85 4.351284182633682E-4 7.911425786606695E-5 0.0 5.0937055641584985 0.001582285157321339 86-87 4.7468554719640166E-4 7.911425786606695E-5 0.0 5.438617357035262 0.0015954708669656836 88-89 4.7468554719640166E-4 7.911425786606695E-5 0.0 5.7883287482225665 0.001608656576610028 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 53.964924 1 GTATCAA 9300 0.0 43.153774 1 ATCAACG 11675 0.0 33.88329 3 TATCAAC 12010 0.0 33.136215 2 TCAACGC 11975 0.0 33.072937 4 CAACGCA 12225 0.0 32.396603 5 AACGCAG 12735 0.0 31.115295 6 ACGCAGA 14505 0.0 27.155977 7 CGCAGAG 14720 0.0 26.727327 8 GCAGAGT 16485 0.0 23.151169 9 GTACATG 11595 0.0 22.939322 1 TACATGG 11215 0.0 22.661722 2 ACATGGG 11350 0.0 21.1032 3 TGGTATC 2180 0.0 20.980637 2 GAGTACT 10085 0.0 20.860668 12-13 AGAGTAC 15010 0.0 20.37234 10-11 CAGAGTA 15865 0.0 19.853554 10-11 GTACTTT 10855 0.0 19.294357 14-15 GTGGTAT 2655 0.0 19.006031 1 AGTACTT 10780 0.0 18.881983 12-13 >>END_MODULE