FastQCFastQC Report
Fri 27 May 2016
SRR939882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3969090
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80830.2036486952928757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59620.1502107535984319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49250.055.1137281
GTATCAA93550.043.245891
ATCAACG118850.033.9225353
TCAACGC120800.033.4930464
TATCAAC121200.033.0314872
CAACGCA122250.032.903785
AACGCAG129050.031.3166086
ACGCAGA145800.027.2993567
TGGTATC22550.026.7032812
CGCAGAG150100.026.485958
GTGGTAT27150.025.1239781
GTACATG114250.024.5403231
TACATGG111900.024.1734682
GCAGAGT162450.023.2560189
ACATGGG117550.022.4115583
AGAGTAC145650.020.41433310-11
GAGTACT102250.019.76176312-13
CAGAGTA155650.019.61661710-11
CATGGGG78800.019.045294
GTGTAGC27350.018.576191
Produced by FastQC (version 0.11.5)