##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939882_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3969090 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.908184999584286 31.0 30.0 33.0 26.0 34.0 2 29.989687560624727 31.0 30.0 33.0 25.0 34.0 3 29.935873714125908 31.0 30.0 34.0 25.0 34.0 4 33.57208352544286 35.0 33.0 37.0 28.0 37.0 5 33.423277123975524 35.0 33.0 37.0 28.0 37.0 6 33.38520013403576 35.0 33.0 37.0 26.0 37.0 7 33.36212658317146 35.0 33.0 37.0 26.0 37.0 8 33.189561335217896 35.0 33.0 37.0 26.0 37.0 9 34.484895026315854 37.0 34.0 39.0 27.0 39.0 10-11 34.27689042072616 37.0 33.0 39.0 26.5 39.0 12-13 34.179546697101856 37.0 33.0 39.0 26.0 39.0 14-15 34.78901209093269 38.0 33.0 40.0 25.0 41.0 16-17 34.55987128031866 38.0 33.0 40.0 25.0 41.0 18-19 34.55247726808916 38.0 33.0 40.0 25.0 41.0 20-21 34.65124726322658 38.0 33.0 40.0 25.0 41.0 22-23 34.562694219581815 38.0 33.0 40.0 24.5 41.0 24-25 34.4052999554054 38.0 33.0 40.0 24.0 41.0 26-27 34.284907875608766 38.0 33.0 40.0 23.5 41.0 28-29 34.15240168401321 38.0 33.0 40.0 22.5 41.0 30-31 33.96074515821007 38.0 32.5 40.0 21.5 41.0 32-33 33.68777515752981 38.0 32.0 40.0 19.0 41.0 34-35 33.40391046814257 37.5 31.0 40.0 18.0 41.0 36-37 33.18932236356444 37.0 31.0 40.0 18.0 41.0 38-39 32.90055844034779 37.0 31.0 40.0 16.0 41.0 40-41 32.60057658052601 37.0 30.5 40.0 15.5 41.0 42-43 32.28450891766123 37.0 30.0 40.0 14.0 41.0 44-45 31.89194714657516 36.5 30.0 39.0 9.0 41.0 46-47 31.52045607431426 36.0 30.0 39.0 9.0 40.0 48-49 31.17878619532437 36.0 29.0 39.0 8.0 40.0 50-51 30.43319564434165 34.5 28.0 38.5 7.0 40.0 52-53 30.53806313285917 35.0 28.0 38.5 4.0 40.0 54-55 30.60398479248392 35.0 28.0 39.0 2.0 40.0 56-57 30.372601906230393 35.0 28.0 39.0 2.0 40.0 58-59 29.81561214283375 35.0 27.0 38.0 2.0 40.0 60-61 29.303384402973983 34.0 26.0 38.0 2.0 40.0 62-63 28.853354169343604 34.0 26.0 38.0 2.0 40.0 64-65 28.31004625745448 34.0 25.5 37.5 2.0 39.5 66-67 27.761689455265564 33.0 24.5 37.0 2.0 39.0 68-69 27.216033901977532 32.5 23.0 36.0 2.0 39.0 70-71 26.664630683607577 32.0 22.0 36.0 2.0 39.0 72-73 26.170685094064382 32.0 20.5 35.0 2.0 38.0 74-75 25.569527020047417 31.0 19.0 35.0 2.0 37.5 76-77 24.91544182671595 31.0 17.5 35.0 2.0 37.0 78-79 24.259740393893814 30.5 11.5 34.0 2.0 36.0 80-81 23.595265161535767 30.0 7.0 34.0 2.0 36.0 82-83 22.838825650212016 29.5 2.0 34.0 2.0 35.0 84-85 22.08361752945889 29.0 2.0 34.0 2.0 35.0 86-87 21.29493750456654 28.5 2.0 33.5 2.0 35.0 88-89 20.432801599359045 27.0 2.0 33.0 2.0 34.5 90-91 19.477606453872298 26.0 2.0 32.5 2.0 34.0 92-93 18.4918910883855 25.0 2.0 32.0 2.0 34.0 94-95 17.12975556109839 20.5 2.0 32.0 2.0 34.0 96-97 14.61940621149936 2.0 2.0 30.5 2.0 34.0 98-99 13.251961154823901 2.0 2.0 29.5 2.0 34.0 100-101 12.012078461309772 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7533761014956859 1101 2 0.7269791182605694 1101 3 0.7861580178680754 1101 4 0.783253590765149 1101 5 0.959487505797938 1101 6 0.9743728624706876 1101 7 0.7483495637352533 1101 8 0.6780837537975799 1101 9 0.892122579042649 1101 10-11 0.9373919448593568 1101 12-13 0.6359532392372813 1101 14-15 0.8492837693921302 1101 16-17 0.8005188693332741 1101 18-19 0.8014321276335821 1101 20-21 0.9000049261230387 1101 22-23 0.7705410657803498 1101 24-25 0.7162244522428978 1101 26-27 0.8235877499461068 1101 28-29 1.0483330270419842 1101 30-31 1.0448031481643199 1101 32-33 1.1145914552454244 1101 34-35 0.804992864296473 1101 36-37 0.8478827003146918 1101 38-39 0.8456160682683063 1101 40-41 0.8607416388665357 1101 42-43 0.796496246649177 1101 44-45 0.739170367704677 1101 46-47 0.6151480240794882 1101 48-49 0.6303525888770061 1101 50-51 0.7427307626271649 1101 52-53 0.8972716014464872 1101 54-55 0.6562568325927316 1101 56-57 0.416161859823184 1101 58-59 0.3308972820049476 1101 60-61 0.407822269992149 1101 62-63 0.12636824351065812 1101 64-65 0.28289256932959717 1101 66-67 0.04979769618491048 1101 68-69 0.18005321994478507 1101 70-71 0.12314762979240257 1101 72-73 0.016957040217281616 1101 74-75 -0.069933315800224 1101 76-77 -0.3636017402599343 1101 78-79 -0.43068642418250747 1101 80-81 -0.8193738079636219 1101 82-83 -0.7938166945875231 1101 84-85 -0.7950140061901223 1101 86-87 -0.7148610575992791 1101 88-89 -0.6548801967413702 1101 90-91 -0.9026930102562574 1101 92-93 -0.8669649918631457 1101 94-95 -0.6292018767539247 1101 96-97 0.7863904855505233 1101 98-99 0.688543007345082 1101 100-101 0.04346010734001382 1102 1 0.9604136960640126 1102 2 0.9243278453073351 1102 3 1.0084641237901444 1102 4 1.0915243403773829 1102 5 1.1510494583130537 1102 6 1.0653585898492821 1102 7 0.9685486170301942 1102 8 0.8890297453336231 1102 9 1.2644923135759143 1102 10-11 1.0758171488553785 1102 12-13 0.9118203652399401 1102 14-15 1.2123702038553574 1102 16-17 1.2119127203917515 1102 18-19 1.176768888206425 1102 20-21 1.023694408801994 1102 22-23 0.9004344246436347 1102 24-25 1.0889762355412316 1102 26-27 1.2193140686378072 1102 28-29 1.2908693944128729 1102 30-31 1.1737788972466063 1102 32-33 1.4030090044112598 1102 34-35 1.2136196154687653 1102 36-37 1.1153583312839785 1102 38-39 1.0382934438461504 1102 40-41 1.1501056852721163 1102 42-43 1.1773570236456194 1102 44-45 1.1358215699979404 1102 46-47 1.2260422329406566 1102 48-49 1.055246720150759 1102 50-51 1.2250247940071226 1102 52-53 1.3011139105589216 1102 54-55 1.1170740082305564 1102 56-57 0.9562478502369807 1102 58-59 0.9605238302030159 1102 60-61 1.2837012419311336 1102 62-63 1.0393729687230326 1102 64-65 0.7510819006438112 1102 66-67 0.5633405109317842 1102 68-69 0.41687146379511475 1102 70-71 0.6587846398226525 1102 72-73 0.625930925816018 1102 74-75 0.6048889085618114 1102 76-77 0.30076426314975535 1102 78-79 0.1604509450359295 1102 80-81 0.03546830510202881 1102 82-83 -0.05842938442558321 1102 84-85 -0.04629922100682293 1102 86-87 -0.13137256097329342 1102 88-89 -0.2927438617909388 1102 90-91 -0.3259657722921787 1102 92-93 -0.365062506779676 1102 94-95 -0.20290164669337685 1102 96-97 0.8760575039006397 1102 98-99 0.9146289714345759 1102 100-101 0.4692794685877306 1103 1 0.8147856682055448 1103 2 0.7074800442681521 1103 3 0.8027101490423085 1103 4 0.812646360443658 1103 5 0.8248249728110366 1103 6 0.858250832987693 1103 7 0.7130506973313544 1103 8 0.6828291186717053 1103 9 0.8706934745903538 1103 10-11 0.9159831922221002 1103 12-13 0.872748642440655 1103 14-15 1.0102513625186447 1103 16-17 1.045890527395649 1103 18-19 0.9420343378406955 1103 20-21 0.8380689257974154 1103 22-23 0.8393634419225862 1103 24-25 0.896042914052714 1103 26-27 1.0094456128698468 1103 28-29 1.1615847722101265 1103 30-31 1.0743156543546633 1103 32-33 1.1376575240209945 1103 34-35 0.9764847201784832 1103 36-37 0.8792190683297747 1103 38-39 0.9903975236944476 1103 40-41 1.1324980683440984 1103 42-43 1.0006564968408256 1103 44-45 1.1380187941701756 1103 46-47 0.9897760946595753 1103 48-49 0.8046438369181423 1103 50-51 1.055921791547096 1103 52-53 1.1521448435584638 1103 54-55 1.1045062373021395 1103 56-57 0.9583504940128726 1103 58-59 0.533236383947191 1103 60-61 0.7991534143747074 1103 62-63 0.7209987563085569 1103 64-65 0.7070898598246238 1103 66-67 0.469095287208404 1103 68-69 0.20918446879999664 1103 70-71 0.44368634233658 1103 72-73 0.24515956791271165 1103 74-75 0.19868995175152193 1103 76-77 0.1317753449531125 1103 78-79 0.07908685659791459 1103 80-81 -0.18938913966428927 1103 82-83 0.029336547795807633 1103 84-85 -0.10792723283471517 1103 86-87 -0.30744485619771567 1103 88-89 -0.3150917199390477 1103 90-91 -0.5333492884520012 1103 92-93 -0.7029771893842955 1103 94-95 -0.6959928025579956 1103 96-97 0.9574356190923492 1103 98-99 1.1502259667703463 1103 100-101 0.5798844291157774 1104 1 0.48815780123895536 1104 2 0.46270477952632305 1104 3 0.5829807198164545 1104 4 0.6369901550594648 1104 5 0.6724580883935758 1104 6 0.7282240763447518 1104 7 0.7184478614353367 1104 8 0.5389569548244992 1104 9 0.7474463941971692 1104 10-11 0.6493369396928017 1104 12-13 0.7220228492422862 1104 14-15 0.7432758517800409 1104 16-17 0.7109001823838881 1104 18-19 0.8535737178671923 1104 20-21 0.6192097294211152 1104 22-23 0.5695055577002819 1104 24-25 0.7039275857317548 1104 26-27 0.9945747483375484 1104 28-29 1.029812991994639 1104 30-31 0.9547416421934898 1104 32-33 0.8449921695083162 1104 34-35 0.9104792661372514 1104 36-37 0.7804780685249284 1104 38-39 0.46822513850150216 1104 40-41 0.6297632158908115 1104 42-43 0.26603161624426974 1104 44-45 0.44503567242488984 1104 46-47 0.18941704978442786 1104 48-49 0.15420775756235372 1104 50-51 0.34392206630340993 1104 52-53 0.397853637814773 1104 54-55 0.26774243015539767 1104 56-57 0.19665332169187622 1104 58-59 0.13195010070681334 1104 60-61 0.4443028741062207 1104 62-63 0.09972799551602662 1104 64-65 0.06805077509696389 1104 66-67 0.09351733601744883 1104 68-69 0.0015289105054385743 1104 70-71 0.08459800689763952 1104 72-73 0.16524874816312618 1104 74-75 0.08758395487398474 1104 76-77 -0.2536067203926713 1104 78-79 -0.14929174733186912 1104 80-81 -0.09173760000180309 1104 82-83 -0.12608103044554397 1104 84-85 -0.1463031148795224 1104 86-87 -0.3188853111120018 1104 88-89 -0.4301946508442569 1104 90-91 -0.4132106777431801 1104 92-93 -0.3477593230339835 1104 94-95 -0.12519233726973056 1104 96-97 1.1063512303866307 1104 98-99 1.2683418446039312 1104 100-101 1.2233935679729964 1105 1 0.5445971248102879 1105 2 0.6790600039554988 1105 3 0.6169098334358161 1105 4 0.6135114478905734 1105 5 0.8168556862458161 1105 6 0.804895971585502 1105 7 0.6549192505980557 1105 8 0.769678148477503 1105 9 0.8284093282512188 1105 10-11 0.7918280056727127 1105 12-13 0.4744056083313666 1105 14-15 0.6912521951040986 1105 16-17 0.7517094028519296 1105 18-19 0.7148792358855474 1105 20-21 0.6614579938041842 1105 22-23 0.5303182501512538 1105 24-25 0.6820834009848156 1105 26-27 0.8028755341717542 1105 28-29 0.7370714276272423 1105 30-31 0.8104946405085585 1105 32-33 0.7398788225617068 1105 34-35 0.8459329685370065 1105 36-37 0.7885510218652669 1105 38-39 0.6876116062825943 1105 40-41 0.5155884741484087 1105 42-43 0.49851317765529046 1105 44-45 0.5382259228571051 1105 46-47 0.5095608118508466 1105 48-49 0.6121529117178959 1105 50-51 0.5313855174719251 1105 52-53 0.7437528401950466 1105 54-55 0.7756078260837995 1105 56-57 0.48206292828537656 1105 58-59 0.4478504529239373 1105 60-61 0.6037824050127334 1105 62-63 0.6620671307188637 1105 64-65 0.4121016553151762 1105 66-67 0.25442136621433775 1105 68-69 0.06209428815630602 1105 70-71 0.1517410818085736 1105 72-73 0.35227295790934576 1105 74-75 0.21256226278356394 1105 76-77 0.2198023973016845 1105 78-79 0.10829836192385756 1105 80-81 0.008712228409081035 1105 82-83 -0.055163687859586474 1105 84-85 -0.08624960813930471 1105 86-87 -0.19220440819169227 1105 88-89 -0.14768438720826538 1105 90-91 -0.13812463349156445 1105 92-93 -0.06065389435583057 1105 94-95 0.10855919459760344 1105 96-97 1.3819990841743248 1105 98-99 1.5699873410388125 1105 100-101 0.9904441440649805 1106 1 1.0889990021501568 1106 2 1.0628350059377283 1106 3 1.0111297435683113 1106 4 0.9827230867863719 1106 5 0.9847452009924993 1106 6 1.0404328345530018 1106 7 1.010508871159253 1106 8 1.1218917670567805 1106 9 1.3585367864961952 1106 10-11 1.3088651292757163 1106 12-13 1.2060235768646876 1106 14-15 1.4563795148317382 1106 16-17 1.6455791029316345 1106 18-19 1.4549063852846231 1106 20-21 1.3779491043343413 1106 22-23 1.1932187523482725 1106 24-25 1.3342456248536791 1106 26-27 1.7231900417776345 1106 28-29 1.599695627530373 1106 30-31 1.588100525871603 1106 32-33 1.7588669708905371 1106 34-35 1.7186511341470663 1106 36-37 1.8342937152346863 1106 38-39 1.707346251635208 1106 40-41 1.7962485554565006 1106 42-43 1.6668977247242864 1106 44-45 1.7331609327491542 1106 46-47 1.7435226848728007 1106 48-49 1.5260671220368955 1106 50-51 1.8479262713437983 1106 52-53 1.9400552869207992 1106 54-55 1.9177959733936483 1106 56-57 1.836336124606479 1106 58-59 1.7959872672989192 1106 60-61 1.971059227303897 1106 62-63 1.8967097766739478 1106 64-65 1.8660826914949524 1106 66-67 1.8012266945784532 1106 68-69 1.7505295867057562 1106 70-71 1.839900619396289 1106 72-73 1.9610931372431324 1106 74-75 1.9686166965963139 1106 76-77 1.8157695683731525 1106 78-79 1.5823944725797894 1106 80-81 1.5553379300312606 1106 82-83 1.7632602013189 1106 84-85 1.829220040482003 1106 86-87 1.747375346113568 1106 88-89 1.7644765756751397 1106 90-91 1.6518718032692163 1106 92-93 1.9230084150116689 1106 94-95 2.2248074698974776 1106 96-97 3.4666514581864334 1106 98-99 3.546160256535911 1106 100-101 2.94160640009534 1107 1 0.5216171854956642 1107 2 0.7091018988185986 1107 3 0.6840644783096721 1107 4 0.3114010191919405 1107 5 0.6240452521579769 1107 6 0.526890910680109 1107 7 0.48315729559344334 1107 8 0.34075560070024835 1107 9 0.6265976912342239 1107 10-11 0.7019050650770424 1107 12-13 0.5637276405746192 1107 14-15 0.7276534716032756 1107 16-17 0.6484321192765847 1107 18-19 0.606410612164062 1107 20-21 0.5776832856739631 1107 22-23 0.5244892599047262 1107 24-25 0.6572655399342509 1107 26-27 0.828128146822138 1107 28-29 0.7968825126218704 1107 30-31 0.8507763276550122 1107 32-33 0.9730049662220068 1107 34-35 0.9596496055465806 1107 36-37 0.5530719230683516 1107 38-39 0.6509807633867197 1107 40-41 0.7097940749133826 1107 42-43 0.7724950944114042 1107 44-45 0.7546725798957219 1107 46-47 0.6797179651756124 1107 48-49 0.6935352963457184 1107 50-51 0.8493296309556548 1107 52-53 0.8159325139555165 1107 54-55 0.7025831848241673 1107 56-57 0.6686207141244331 1107 58-59 0.52091974668636 1107 60-61 0.7637254451059192 1107 62-63 0.7850258469615703 1107 64-65 0.872878186132759 1107 66-67 0.8280446600252098 1107 68-69 0.6018481796296484 1107 70-71 0.7078906306730595 1107 72-73 0.6259673681699915 1107 74-75 0.594152724984248 1107 76-77 0.6263474900874222 1107 78-79 0.4325435461282545 1107 80-81 0.29663722135120096 1107 82-83 0.5271102635575033 1107 84-85 0.4262749708231297 1107 86-87 0.41058151244464725 1107 88-89 0.37407735766692696 1107 90-91 0.3884512383106973 1107 92-93 0.47865742746702367 1107 94-95 0.7374735349667318 1107 96-97 2.162176419812326 1107 98-99 2.046867727459409 1107 100-101 1.596670223302068 1108 1 0.3995633341734717 1108 2 0.39621632819871166 1108 3 0.46853308786147707 1108 4 0.4647349797497 1108 5 0.7025479722103043 1108 6 0.6291125658826857 1108 7 0.5880583648961561 1108 8 0.6180622760101357 1108 9 0.6134811476808721 1108 10-11 0.5946650808003398 1108 12-13 0.3858672755102859 1108 14-15 0.48347064980265486 1108 16-17 0.5948860764059631 1108 18-19 0.5650059184236369 1108 20-21 0.48590122284775106 1108 22-23 0.43039771403623206 1108 24-25 0.5670349955494274 1108 26-27 0.564541184946215 1108 28-29 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94-95 0.7100845980234745 1108 96-97 2.1976997762051873 1108 98-99 2.2537914477737946 1108 100-101 1.791923012808832 1109 1 0.42092841544375403 1109 2 0.4084493907242397 1109 3 -0.6187396822625644 1109 4 0.2253315692575626 1109 5 0.3680191386746188 1109 6 0.19313188113586932 1109 7 0.3471750157008415 1109 8 0.2782103646392926 1109 9 0.5511173786990398 1109 10-11 0.43849018173672505 1109 12-13 0.2744830578137609 1109 14-15 0.31385305517562756 1109 16-17 0.3729325974080382 1109 18-19 0.26712169501728056 1109 20-21 0.29103659313977914 1109 22-23 0.3575275586730413 1109 24-25 0.4910815029451143 1109 26-27 0.3860052041642845 1109 28-29 0.46248850281457266 1109 30-31 0.3913905730480636 1109 32-33 0.32051761647883836 1109 34-35 0.5108061682541845 1109 36-37 0.3914407966130611 1109 38-39 0.45439711865058285 1109 40-41 0.4280697664371971 1109 42-43 0.25353381306783973 1109 44-45 0.5537050582299337 1109 46-47 0.2385236564021227 1109 48-49 0.08992503830582521 1109 50-51 0.13660840986348077 1109 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0.7742343530055216 2312 50-51 0.5489998757770138 2312 52-53 0.4450177707048333 2312 54-55 0.391324443459137 2312 56-57 0.33362428245801823 2312 58-59 0.6659632705350305 2312 60-61 0.7666838765918129 2312 62-63 0.8514540245439228 2312 64-65 0.7931802095602976 2312 66-67 0.8194908312687446 2312 68-69 0.7341731430460463 2312 70-71 0.8433846531297071 2312 72-73 0.9601809469653908 2312 74-75 0.9562386194036634 2312 76-77 0.918901768042371 2312 78-79 1.1483205437520176 2312 80-81 1.01509029378931 2312 82-83 1.0748596412036484 2312 84-85 1.1717483783198084 2312 86-87 1.0415386536248548 2312 88-89 1.0548899182011553 2312 90-91 1.4528736312641968 2312 92-93 1.501912919216597 2312 94-95 1.9466208505556715 2312 96-97 3.55370203090699 2312 98-99 3.8189354505024706 2312 100-101 3.4492664490527165 2313 1 -0.023292931268539263 2313 2 -0.4104257708441743 2313 3 -0.8328523306954523 2313 4 0.03225360321829385 2313 5 -0.059258213433452056 2313 6 0.07950667151416013 2313 7 0.06981318187201424 2313 8 0.33335143415025215 2313 9 0.4040340122751118 2313 10-11 -0.5451148109718318 2313 12-13 0.1658934018753584 2313 14-15 0.22547895998751954 2313 16-17 -0.3688681868677648 2313 18-19 0.14166481464869207 2313 20-21 -2.033350511542743 2313 22-23 -0.13595324935894837 2313 24-25 0.32909197568594806 2313 26-27 0.4033330208642738 2313 28-29 0.24242930230629867 2313 30-31 0.5406079949283722 2313 32-33 0.5782438381547337 2313 34-35 0.4554309036733173 2313 36-37 -0.465652934359845 2313 38-39 0.510013771908369 2313 40-41 0.6832737368478732 2313 42-43 0.6880885057742177 2313 44-45 0.6519616156343453 2313 46-47 0.5947924245228151 2313 48-49 0.419889145084273 2313 50-51 0.7675592205542152 2313 52-53 0.6510134602029147 2313 54-55 0.6522176549984273 2313 56-57 0.6034509346790351 2313 58-59 0.7009701951691696 2313 60-61 0.4697041270051372 2313 62-63 0.6638139166654149 2313 64-65 0.494955930931571 2313 66-67 0.7954755208695374 2313 68-69 0.5883656942601725 2313 70-71 0.8985753953245847 2313 72-73 0.7076146908069383 2313 74-75 0.9416534016752749 2313 76-77 1.0888596238575694 2313 78-79 0.9595711691226825 2313 80-81 1.0579947802233214 2313 82-83 1.1843762918011898 2313 84-85 1.1920241377931404 2313 86-87 1.2037987349313255 2313 88-89 1.4966468701693785 2313 90-91 1.7676635232862878 2313 92-93 1.7049653078840556 2313 94-95 2.1109897148825425 2313 96-97 3.600592986142047 2313 98-99 3.8822358007100473 2313 100-101 3.4433349532323447 2314 1 -0.3225340248890838 2314 2 -0.2252156079057599 2314 3 -1.2153461169423458 2314 4 -0.36810044262539776 2314 5 -0.550520295731161 2314 6 -0.5771865071657629 2314 7 -0.351194826965326 2314 8 -1.801805152428038 2314 9 -1.4530507709339417 2314 10-11 -0.8448096466525783 2314 12-13 -0.6534457309608754 2314 14-15 -0.6865414763560338 2314 16-17 -0.6841810838575526 2314 18-19 -1.8224464805736318 2314 20-21 -1.3130563938956854 2314 22-23 -1.8821045887679446 2314 24-25 -1.717921280320688 2314 26-27 -1.8434239523475497 2314 28-29 -2.87652264466967 2314 30-31 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2314 96-97 3.0575123454350575 2314 98-99 3.0726044833943877 2314 100-101 2.717495682312709 2315 1 -2.4834471901551893 2315 2 -1.749745019670467 2315 3 -1.6294217471944457 2315 4 -1.7432292941660101 2315 5 -1.8619208559552511 2315 6 -2.8318447704710827 2315 7 -2.178925919402296 2315 8 -1.192004031979863 2315 9 -1.011123600065595 2315 10-11 -3.591714408557344 2315 12-13 -1.8918799046303505 2315 14-15 -1.2970260701935672 2315 16-17 -1.945053632877162 2315 18-19 -1.3305585253915524 2315 20-21 -0.9812076543998813 2315 22-23 -1.2341340005326487 2315 24-25 -2.9737266024495383 2315 26-27 -4.185786697445586 2315 28-29 -5.997040851952583 2315 30-31 -8.742502333710128 2315 32-33 -6.920849052522669 2315 34-35 -6.067627446427288 2315 36-37 -3.935270640597274 2315 38-39 -6.415963759949449 2315 40-41 -4.077465699771842 2315 42-43 -4.015171051569844 2315 44-45 -3.1510866741040893 2315 46-47 -2.408334671385969 2315 48-49 -3.0921799360693143 2315 50-51 -2.809100739204336 2315 52-53 -2.0978161843309557 2315 54-55 -3.506682412070653 2315 56-57 -3.448903190069455 2315 58-59 -2.016274942322447 2315 60-61 -2.2489293404663577 2315 62-63 -3.0332367204908586 2315 64-65 -2.263930722321277 2315 66-67 -2.6645814878494463 2315 68-69 -1.9548049965513634 2315 70-71 -2.4990688031998936 2315 72-73 -1.5919342696223033 2315 74-75 -2.222907295843168 2315 76-77 -1.3631893365716685 2315 78-79 -1.2229707490530366 2315 80-81 -0.9424210480798294 2315 82-83 -0.6644416156434794 2315 84-85 -0.6225667408118234 2315 86-87 -0.9158323851223322 2315 88-89 -1.2576924993812604 2315 90-91 -1.1914981131992874 2315 92-93 -0.49332946072761885 2315 94-95 0.05816107638488788 2315 96-97 1.5347462389924829 2315 98-99 1.6528075646268796 2315 100-101 1.4221679512202723 2316 1 -3.6650205305612076 2316 2 -2.2981263146344943 2316 3 -2.4742695740021254 2316 4 -3.180284571603046 2316 5 -2.664332972345086 2316 6 -5.876344930711554 2316 7 -3.5704997018509914 2316 8 -4.63517121252114 2316 9 -6.527274734457624 2316 10-11 -5.8773740467454125 2316 12-13 -3.7314522835935335 2316 14-15 -5.039205285346789 2316 16-17 -4.618893464237878 2316 18-19 -2.7754580159373674 2316 20-21 -3.633043015604006 2316 22-23 -4.3133258083767 2316 24-25 -3.213771552664479 2316 26-27 -4.248833614750453 2316 28-29 -4.01861170586853 2316 30-31 -3.6176843171556285 2316 32-33 -3.5794384667056107 2316 34-35 -5.4760229972677195 2316 36-37 -5.840137254218821 2316 38-39 -5.701606572888039 2316 40-41 -5.097337866554547 2316 42-43 -4.459989869326698 2316 44-45 -3.811690670169906 2316 46-47 -3.8282463457331275 2316 48-49 -4.393337756356864 2316 50-51 -5.391315152005891 2316 52-53 -3.249564242105933 2316 54-55 -3.646812925171691 2316 56-57 -2.807210940182724 2316 58-59 -3.4161642935143064 2316 60-61 -3.4153319695151936 2316 62-63 -2.5268528230005565 2316 64-65 -3.0628770030846866 2316 66-67 -3.714255149841513 2316 68-69 -3.120758510238108 2316 70-71 -2.3915879215457743 2316 72-73 -2.4576027762916226 2316 74-75 -3.3845781619152895 2316 76-77 -3.746896251224282 2316 78-79 -3.742662037496938 2316 80-81 -3.8332287153100992 2316 82-83 -2.8715348811232637 2316 84-85 -2.7444545393287676 2316 86-87 -2.3257360198182617 2316 88-89 -2.4192905389219774 2316 90-91 -1.945535731031292 2316 92-93 -1.597177248856557 2316 94-95 -1.6737277996110898 2316 96-97 -0.13642155488301633 2316 98-99 0.3527213386767638 2316 100-101 0.196422169965091 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37610.0 3 12598.0 4 12350.0 5 13560.0 6 15232.0 7 17909.0 8 22090.0 9 26700.0 10 31100.0 11 36260.0 12 41441.0 13 44965.0 14 48481.0 15 52723.0 16 57161.0 17 60366.0 18 58634.0 19 60493.0 20 65387.0 21 71437.0 22 79014.0 23 87983.0 24 97690.0 25 109158.0 26 121481.0 27 136377.0 28 152647.0 29 171562.0 30 192405.0 31 217882.0 32 247626.0 33 282013.0 34 319072.0 35 346302.0 36 335130.0 37 234028.0 38 51764.0 39 459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04292743861355 18.357473543054954 13.652522746300644 23.94707627203085 2 15.864832605883766 19.30112376328529 40.39796838772558 24.436075243105375 3 19.734411697186953 23.3904385183765 29.01254531373375 27.862604470702795 4 13.090475216093903 15.970802124114941 36.55746940977267 34.38125325001848 5 14.308875112934263 36.75822594118613 34.05393445183141 14.878964494048198 6 33.30998736491969 35.48953871864946 17.115412174081534 14.085061742349318 7 29.397455325896015 31.84862050363615 20.273976612171097 18.479947558296736 8 26.556551692120127 35.319021061173075 19.201920010334792 18.922507236372006 9 26.51200171588264 16.63628965612955 19.599719907392803 37.25198872059501 10-11 25.43484793852997 25.939570550521847 27.254226228670152 21.37135528227804 12-13 26.282505702135023 23.151254848186767 27.453148124808475 23.113091324869735 14-15 23.107849303583993 24.729460288002148 25.40289243427335 26.759797974140508 16-17 22.98204931669428 27.784957131604536 26.14437246027905 23.08862109142213 18-19 22.918168118153886 26.551806889618334 28.11248369852671 22.41754129370107 20-21 23.439074092912296 25.92499710344817 28.262760541366166 22.373168262273367 22-23 23.101321029809956 25.834091784116087 28.118558165075076 22.946029020998882 24-25 22.84527923301401 25.99919522732177 28.320738401027544 22.834787138636678 26-27 22.738513371384936 26.24608560322682 28.1184936292648 22.896907396123446 28-29 22.729039271811747 26.50250696660198 28.093644767062337 22.674808994523943 30-31 22.90292823380214 26.21125061174273 27.96625149810682 22.919569656348312 32-33 22.583779397933057 26.26738723887181 28.031619922531036 23.1172134406641 34-35 23.149755783226137 26.419767579095122 27.787343644778357 22.643132992900387 36-37 23.231226972618053 26.04547721772178 27.864053505816354 22.859242303843814 38-39 22.86090449787461 26.344272176165966 27.837189120274676 22.957634205684748 40-41 23.243234237706822 26.1778854466977 27.83357276121051 22.745307554384965 42-43 22.919557323000955 26.154551227300153 27.77207342306006 23.153818026638824 44-45 22.982267581109234 26.344705802906788 27.864227115321626 22.808799500662357 46-47 23.145990105853606 26.54284991058404 27.368090146947704 22.943069836614654 48-49 23.00927787862047 26.60814773893142 27.307243311498695 23.07533107094941 50-51 22.94669404521603 26.815111817563785 27.273768612528148 22.964425524692032 52-53 23.158694941486 26.559484896915215 27.240362912618316 23.04145724898047 54-55 22.836865260716504 26.669151623771263 27.393229051868452 23.100754063643787 56-57 22.753939125170877 26.82967821544011 27.26038014505648 23.15600251433253 58-59 22.837301692521798 26.552097009330172 27.268608762385576 23.341992535762454 60-61 22.70606916674546 26.660234543179325 27.407119964320774 23.226576325754444 62-63 22.715094412975418 26.606918989986404 27.497113258713114 23.18087333832507 64-65 22.4783677990373 26.639280224941064 27.390156581820698 23.492195394200937 66-67 22.50409541238089 26.709370858837634 27.251063292736795 23.53547043604468 68-69 22.75080273029272 26.4261210456698 27.397904318588 23.425171905449478 70-71 22.613530181628345 26.415849512231727 27.201918230829147 23.76870207531078 72-73 22.713716884276238 26.544512368738214 27.088871955165338 23.652898791820213 74-75 22.662051796312777 26.74937670078948 26.981092594297408 23.607478908600335 76-77 22.74088719775663 26.453050492221035 27.087439806461177 23.718622503561154 78-79 22.837194353041614 26.568172619058895 26.955190196817604 23.63944283108189 80-81 22.878730662315306 26.584127216674265 26.780355223590302 23.756786897420128 82-83 22.875228118034112 26.601259148401436 26.85993394838376 23.663578785180693 84-85 22.93327390002071 26.585758229325034 26.77712760315384 23.703840267500418 86-87 22.86874270443829 26.496722560638787 26.817806189477217 23.816728545445706 88-89 22.938601419969217 26.515999590903412 26.547868720398892 23.99753026872848 90-91 22.939377161144257 26.566974401192972 26.54413264130419 23.949515796358575 92-93 23.09569179209175 26.27423422194778 26.67191980876542 23.95815417719505 94-95 22.97790298955393 26.12594307152221 26.74996176758404 24.14619217133982 96-97 23.236743893510138 25.791331595225696 26.682698212023194 24.28922629924098 98-99 23.082713331994746 25.925623595259285 26.423903378226488 24.567759694519477 100-101 23.218092557846283 25.938229306509324 26.48816598594373 24.355512149700658 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4689.0 1 2648.5 2 573.0 3 517.5 4 449.5 5 440.0 6 557.0 7 893.5 8 1370.5 9 1663.0 10 1614.0 11 1578.0 12 1680.5 13 1788.0 14 2031.0 15 2390.0 16 2713.5 17 3006.0 18 3449.5 19 4092.0 20 4855.5 21 5885.0 22 7239.0 23 9029.5 24 11520.0 25 14566.0 26 19004.5 27 24504.0 28 29278.5 29 34032.0 30 39914.5 31 47909.0 32 57452.5 33 63695.5 34 68116.0 35 74608.0 36 83053.5 37 91439.0 38 97245.5 39 102059.0 40 108835.0 41 116071.5 42 121343.0 43 126803.5 44 132822.0 45 138341.0 46 142118.0 47 146507.5 48 149668.0 49 149602.0 50 149564.0 51 149419.0 52 148851.5 53 148484.0 54 146667.5 55 139581.0 56 129295.0 57 118789.0 58 109727.5 59 98089.5 60 82212.0 61 67871.5 62 55706.0 63 43598.0 64 32601.0 65 24513.5 66 18249.5 67 13414.0 68 9930.0 69 7406.5 70 5607.0 71 4187.5 72 3149.5 73 2419.5 74 1815.5 75 1385.0 76 992.0 77 668.5 78 474.5 79 334.5 80 237.5 81 157.0 82 106.0 83 82.5 84 58.0 85 35.5 86 20.5 87 15.5 88 11.0 89 8.0 90 7.0 91 4.5 92 6.5 93 7.5 94 3.5 95 1.5 96 1.0 97 2.0 98 2.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12141322066267078 2 0.1147366272873631 3 0.12748514143040346 4 0.1423752043919387 5 0.13859600059459473 6 0.13912508912622287 7 0.14693544364073377 8 0.14557493027368995 9 0.15406554147172272 10-11 0.14922816061112243 12-13 0.14818258089385727 14-15 0.13703392969169254 16-17 0.1401832661894792 18-19 0.15766838242519066 20-21 0.18853188010349978 22-23 0.20571466003542374 24-25 0.22588301096724941 26-27 0.22748287390812502 28-29 0.22990156433842518 30-31 0.2290701395030095 32-33 0.23132506443542472 34-35 0.20048676144909788 36-37 0.18901057925116335 38-39 0.20288025718741576 40-41 0.19054745546208324 42-43 0.19378497338180792 44-45 0.1975893718711342 46-47 0.21837499275652608 48-49 0.23689309136351155 50-51 0.25269016323640936 52-53 0.2744205850711372 54-55 0.26717711112622794 56-57 0.23660335240571514 58-59 0.23030467941014188 60-61 0.20764205397206917 62-63 0.198433394052541 64-65 0.18943888901486236 66-67 0.18492903915003187 68-69 0.19173160598525102 70-71 0.19479276106109966 72-73 0.23198012642696436 74-75 0.2447790299539693 76-77 0.26204999130783124 78-79 0.2511280923335072 80-81 0.25635599091983297 82-83 0.25358457480178076 84-85 0.23859373307231632 86-87 0.22832689608953183 88-89 0.230405458178071 90-91 0.23273596718643316 92-93 0.24073528189081125 94-95 0.24573642824929645 96-97 8.70751230130836 98-99 0.3067831669223928 100-101 0.318322335850283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3969090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91759532740394 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48494102096292 41.45451444302194 2 12.770166577328087 14.026136807144859 3 4.825588742904571 7.950291893979272 4 2.2954836842338144 5.042497762056435 5 1.3189592838434305 3.6217036101718003 6 0.7880373395043846 2.596626942827149 7 0.5131404523223513 1.9726307794731928 8 0.3773806646693021 1.6579870901356364 9 0.24762451620450807 1.2239048676657016 >10 1.2634089593920566 12.225704830021503 >50 0.07826132914629676 2.9600082848499016 >100 0.033886144119332856 3.185165359208996 >500 0.0019566266892431026 0.744804398218778 >1k 0.0010714859855315347 0.9786942238106536 >5k 9.317269439404511E-5 0.35932870741413986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8083 0.2036486952928757 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5962 0.1502107535984319 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5194691982293172E-5 2 2.5194691982293172E-5 0.0 0.0 0.0 2.5194691982293172E-5 3 2.5194691982293172E-5 0.0 0.0 0.0 2.5194691982293172E-5 4 2.5194691982293172E-5 0.0 0.0 0.0 2.5194691982293172E-5 5 2.5194691982293172E-5 0.0 0.0 0.0 2.5194691982293172E-5 6 5.0389383964586344E-5 0.0 0.0 0.0 1.0077876792917269E-4 7 5.0389383964586344E-5 0.0 0.0 7.558407594687951E-5 1.0077876792917269E-4 8 1.0077876792917269E-4 0.0 0.0 7.558407594687951E-5 1.0077876792917269E-4 9 1.0077876792917269E-4 0.0 0.0 7.558407594687951E-5 1.0077876792917269E-4 10-11 1.0077876792917269E-4 0.0 0.0 7.558407594687951E-5 1.5116815189375902E-4 12-13 1.0077876792917269E-4 0.0 0.0 1.0077876792917269E-4 3.65323033743251E-4 14-15 1.2597345991146585E-4 0.0 0.0 1.0077876792917269E-4 4.7869914766357025E-4 16-17 1.2597345991146585E-4 1.2597345991146586E-5 0.0 1.2597345991146585E-4 5.542832236104497E-4 18-19 1.2597345991146585E-4 2.5194691982293172E-5 0.0 1.2597345991146585E-4 5.542832236104497E-4 20-21 1.2597345991146585E-4 2.5194691982293172E-5 0.0 1.5116815189375902E-4 5.920752615838895E-4 22-23 1.2597345991146585E-4 2.5194691982293172E-5 0.0 2.8973895779637145E-4 6.172699535661827E-4 24-25 1.5116815189375902E-4 2.5194691982293172E-5 0.0 7.054513755042087E-4 6.550619915396224E-4 26-27 1.5116815189375902E-4 2.5194691982293172E-5 0.0 0.0011589558311854858 6.67659337530769E-4 28-29 1.637654978849056E-4 2.5194691982293172E-5 0.0 0.004862575552582582 6.928540295130622E-4 30-31 2.3934957383178512E-4 5.0389383964586344E-5 0.0 0.017825244577472418 7.180487214953553E-4 32-33 2.519469198229317E-4 5.0389383964586344E-5 0.0 0.04696290585499447 8.062301434333815E-4 34-35 2.519469198229317E-4 5.0389383964586344E-5 0.0 0.08964271407299909 8.062301434333815E-4 36-37 2.519469198229317E-4 5.0389383964586344E-5 0.0 0.16597003343335626 8.062301434333815E-4 38-39 2.7714161180522487E-4 5.0389383964586344E-5 0.0 0.2833269086868778 8.314248354156747E-4 40-41 2.7714161180522487E-4 5.0389383964586344E-5 0.0 0.4166068292732087 8.314248354156747E-4 42-43 2.8973895779637145E-4 5.0389383964586344E-5 0.0 0.5531746571632289 8.314248354156747E-4 44-45 3.0233630378751804E-4 5.0389383964586344E-5 0.0 0.7151639292633828 9.070089113625542E-4 46-47 3.6532303374325095E-4 5.0389383964586344E-5 0.0 0.911644734687296 9.825929873094337E-4 48-49 3.7792037973439753E-4 5.0389383964586344E-5 0.0 1.119954447996896 9.825929873094337E-4 50-51 3.7792037973439753E-4 5.0389383964586344E-5 0.0 1.3445651270190395 0.0010077876792917268 52-53 3.7792037973439753E-4 5.0389383964586344E-5 0.0 1.5603324691553984 0.00103298237127402 54-55 3.7792037973439753E-4 5.0389383964586344E-5 0.0 1.7872358651479305 0.0010707744092474597 56-57 3.7792037973439753E-4 5.0389383964586344E-5 0.0 2.0471065155992934 0.0010833717552386063 58-59 3.7792037973439753E-4 5.0389383964586344E-5 0.0 2.3274352559402787 0.0010833717552386063 60-61 3.7792037973439753E-4 5.0389383964586344E-5 0.0 2.630300648259425 0.0010833717552386063 62-63 3.7792037973439753E-4 5.0389383964586344E-5 0.0 2.9302938456925895 0.001184150523167779 64-65 3.7792037973439753E-4 5.0389383964586344E-5 0.0 3.2243033037799593 0.0012093452151500721 66-67 3.7792037973439753E-4 5.0389383964586344E-5 0.0 3.5646332030767756 0.0012093452151500721 68-69 3.7792037973439753E-4 5.0389383964586344E-5 0.0 3.942415012005271 0.0012093452151500721 70-71 3.9051772572554417E-4 5.0389383964586344E-5 0.0 4.331735989861656 0.001272331945105805 72-73 4.0311507171669075E-4 5.0389383964586344E-5 0.0 4.7311600392029405 0.0013857080590261243 74-75 4.0311507171669075E-4 5.0389383964586344E-5 0.0 5.1200904993336005 0.0013983054050172709 76-77 4.0311507171669075E-4 5.0389383964586344E-5 0.0 5.532993708885412 0.001423500096999564 78-79 4.0311507171669075E-4 5.0389383964586344E-5 0.0 5.989710487794431 0.0014360974429907106 80-81 4.283097636989839E-4 5.0389383964586344E-5 0.0 6.466595617635276 0.0014612921349730038 82-83 4.283097636989839E-4 5.0389383964586344E-5 0.0 6.954175894222605 0.0015998629408756164 84-85 4.283097636989839E-4 5.0389383964586344E-5 0.0 7.422331063291586 0.001612460286866763 86-87 4.283097636989839E-4 5.0389383964586344E-5 0.0 7.840562950197652 0.0016628496708313493 88-89 4.283097636989839E-4 5.0389383964586344E-5 0.0 8.262435470095161 0.0017132390547959357 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4925 0.0 55.113728 1 GTATCAA 9355 0.0 43.24589 1 ATCAACG 11885 0.0 33.922535 3 TCAACGC 12080 0.0 33.493046 4 TATCAAC 12120 0.0 33.031487 2 CAACGCA 12225 0.0 32.90378 5 AACGCAG 12905 0.0 31.316608 6 ACGCAGA 14580 0.0 27.299356 7 TGGTATC 2255 0.0 26.703281 2 CGCAGAG 15010 0.0 26.48595 8 GTGGTAT 2715 0.0 25.123978 1 GTACATG 11425 0.0 24.540323 1 TACATGG 11190 0.0 24.173468 2 GCAGAGT 16245 0.0 23.256018 9 ACATGGG 11755 0.0 22.411558 3 AGAGTAC 14565 0.0 20.414333 10-11 GAGTACT 10225 0.0 19.761763 12-13 CAGAGTA 15565 0.0 19.616617 10-11 CATGGGG 7880 0.0 19.04529 4 GTGTAGC 2735 0.0 18.57619 1 >>END_MODULE