FastQCFastQC Report
Fri 27 May 2016
SRR939882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3969090
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92560.23320206898810558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74710.18822954379971227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49810.12549476076380228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.055.0763171
GTATCAA95500.043.436691
ATCAACG123850.033.135093
TCAACGC124750.032.934074
TATCAAC127900.032.3157422
CAACGCA128150.032.1343275
AACGCAG135100.030.4812286
ACGCAGA153650.026.6468757
CGCAGAG157550.025.896928
TGGTATC23500.023.4237632
GTACATG118000.022.9266951
TACATGG114350.022.9070662
GCAGAGT175100.022.5426649
GTGGTAT26300.022.3776571
TAAGGTG20500.021.2912925
AGAGTAC157000.021.06207810-11
ACATGGG118550.020.8098953
GAGTACT110950.020.16148212-13
CAGAGTA167400.020.022810-11
GTAAGGT18700.019.2814664
Produced by FastQC (version 0.11.5)