##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939882_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3969090 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.378786321297827 31.0 31.0 34.0 30.0 34.0 2 31.620708273181005 31.0 31.0 34.0 30.0 34.0 3 31.666980340581848 31.0 31.0 34.0 30.0 34.0 4 35.47461030110176 37.0 35.0 37.0 33.0 37.0 5 35.20243456308625 37.0 35.0 37.0 32.0 37.0 6 35.22004464499419 37.0 35.0 37.0 32.0 37.0 7 35.12761262657183 37.0 35.0 37.0 32.0 37.0 8 35.08535382165685 37.0 35.0 37.0 32.0 37.0 9 36.678353476489576 39.0 35.0 39.0 32.0 39.0 10-11 36.539373004895324 38.0 35.0 39.0 32.0 39.0 12-13 36.45849338261415 38.0 35.0 39.0 32.0 39.0 14-15 37.50651282787742 39.0 36.0 41.0 32.0 41.0 16-17 37.41787865732448 39.0 36.0 41.0 32.0 41.0 18-19 37.37992612916311 39.0 36.0 41.0 32.0 41.0 20-21 37.2832319246981 39.0 36.0 40.5 31.0 41.0 22-23 37.1578565867743 39.0 36.0 40.0 31.0 41.0 24-25 37.0062558420192 39.0 36.0 40.0 31.0 41.0 26-27 36.697984676588334 38.5 35.5 40.0 30.0 41.0 28-29 36.46762419093545 38.0 35.0 40.0 30.0 41.0 30-31 36.20536407589649 38.0 35.0 40.0 30.0 41.0 32-33 36.19805824508892 38.0 35.0 40.0 30.0 41.0 34-35 36.05997533439655 38.0 35.0 40.0 29.5 41.0 36-37 36.118017983971136 38.0 35.0 40.0 30.0 41.0 38-39 36.01966206359644 38.0 35.0 40.0 29.0 41.0 40-41 35.93043380220655 38.0 35.0 40.0 29.0 41.0 42-43 35.709418657677205 38.0 34.0 40.0 28.0 41.0 44-45 35.47403825562031 38.0 34.0 40.0 27.0 41.0 46-47 35.21389298302634 38.0 34.0 40.0 27.0 41.0 48-49 34.91574025784247 38.0 33.5 40.0 26.0 41.0 50-51 34.68856463320307 38.0 33.0 40.0 26.0 41.0 52-53 34.430179713737914 38.0 33.0 40.0 24.0 41.0 54-55 34.151686910601676 37.0 33.0 40.0 24.0 41.0 56-57 33.770750852210455 37.0 32.5 40.0 23.5 41.0 58-59 33.38868720537957 36.5 32.0 39.0 23.0 41.0 60-61 33.063102877485775 36.0 31.0 39.0 22.0 40.0 62-63 32.7540652895248 36.0 31.0 39.0 22.0 40.0 64-65 32.30333993434263 35.0 31.0 38.5 20.5 40.0 66-67 31.40023116129894 35.0 29.5 38.0 15.5 40.0 68-69 29.533240490893377 34.0 26.5 37.0 8.5 39.5 70-71 28.87750088811289 33.0 25.5 36.0 8.5 39.0 72-73 29.415947610157495 33.5 27.0 36.0 9.0 39.0 74-75 29.499378446948796 34.0 28.0 36.0 8.0 38.5 76-77 29.0558649463731 32.5 27.5 35.0 9.0 37.0 78-79 29.593902002726068 34.0 29.0 35.0 8.0 37.0 80-81 29.419088128513085 34.0 29.0 35.0 7.0 37.0 82-83 28.979617116265945 33.5 28.5 35.0 6.0 36.5 84-85 28.624567848045775 33.0 28.0 35.0 2.0 36.0 86-87 28.24304286372947 33.0 27.0 35.0 2.0 35.5 88-89 27.785832772751437 33.0 26.5 35.0 2.0 35.0 90-91 27.28788815572335 32.5 26.0 35.0 2.0 35.0 92-93 26.790290343630403 32.0 25.0 34.0 2.0 35.0 94-95 25.97016205225883 32.0 24.0 34.0 2.0 35.0 96-97 25.424421592858817 32.0 22.0 34.0 2.0 35.0 98-99 24.73797621117183 31.5 19.0 34.0 2.0 35.0 100-101 23.240208082961082 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2651421654581618 1101 2 0.34037759164160164 1101 3 0.32737324070006935 1101 4 0.1937044060074058 1101 5 0.2739675298779858 1101 6 0.18372647147182875 1101 7 0.1509039242006196 1101 8 0.18339811433345687 1101 9 0.29852510134512755 1101 10-11 0.3608762904181333 1101 12-13 0.296344829978203 1101 14-15 0.29126255253567024 1101 16-17 0.42093274063617514 1101 18-19 0.2437718050230373 1101 20-21 0.3617109739213191 1101 22-23 0.3378096936186168 1101 24-25 0.2986229602136987 1101 26-27 0.2883608782414697 1101 28-29 0.23582122624783608 1101 30-31 0.10435509401695953 1101 32-33 0.11609224301589904 1101 34-35 0.17958023084175778 1101 36-37 0.4125207666429205 1101 38-39 0.33154187037323624 1101 40-41 0.27923233757193344 1101 42-43 0.1699804895803183 1101 44-45 0.006744778276782881 1101 46-47 -0.01745292225776751 1101 48-49 0.09327057695886509 1101 50-51 0.16064930672183664 1101 52-53 -8.851024938749674E-4 1101 54-55 -0.23772059312337035 1101 56-57 -0.1468816021341226 1101 58-59 -0.14182129776397545 1101 60-61 -0.2681163314822115 1101 62-63 -0.1319670542630007 1101 64-65 -0.22893481471029276 1101 66-67 -0.2350487216756285 1101 68-69 -8.942504992866336 1101 70-71 -6.962402789645708 1101 72-73 -1.738469294339847 1101 74-75 -0.41479226698489313 1101 76-77 -0.219851359696289 1101 78-79 -0.44822718967791175 1101 80-81 -0.46237726450364036 1101 82-83 -0.43674035087230934 1101 84-85 -0.3956277477259462 1101 86-87 -0.5016723613442906 1101 88-89 -0.3991882168464542 1101 90-91 -0.8013958021989573 1101 92-93 -0.9804888861984331 1101 94-95 -0.6711384818159409 1101 96-97 -1.178444705810648 1101 98-99 -1.0753343306210716 1101 100-101 -1.0738281940186205 1102 1 0.2464528802114785 1102 2 0.22794677087061643 1102 3 0.33193932959446926 1102 4 0.17538580851084618 1102 5 0.3124202704803878 1102 6 0.27644634694402015 1102 7 0.32728485903204074 1102 8 0.30223329163802504 1102 9 0.35036652097322474 1102 10-11 0.37741145102755524 1102 12-13 0.3261804485612174 1102 14-15 0.483223248965885 1102 16-17 0.5532836810026183 1102 18-19 0.36927634838989576 1102 20-21 0.28083769288098637 1102 22-23 0.30464339260574747 1102 24-25 0.3150296166600697 1102 26-27 0.35465995141817785 1102 28-29 0.2650889672693424 1102 30-31 0.11850710855350854 1102 32-33 0.156126139019662 1102 34-35 0.13770221350512912 1102 36-37 0.24192189567842348 1102 38-39 0.3921824307206663 1102 40-41 0.27318786196936173 1102 42-43 0.31425897161017247 1102 44-45 0.35186037314022656 1102 46-47 0.333741508351018 1102 48-49 0.39769084306986 1102 50-51 0.24113063274624835 1102 52-53 0.3058039375211479 1102 54-55 0.1037301392118124 1102 56-57 0.2161043150565547 1102 58-59 0.05995559268467332 1102 60-61 0.2901065494633528 1102 62-63 0.28648389213032743 1102 64-65 0.21846967829402075 1102 66-67 0.41186357613813485 1102 68-69 -4.481319623359713 1102 70-71 0.2944291636244962 1102 72-73 0.6824298122271664 1102 74-75 0.4822814057333602 1102 76-77 0.26129867289735387 1102 78-79 0.05927398972013265 1102 80-81 0.13618733360391744 1102 82-83 0.15191200414832196 1102 84-85 0.12121810893268048 1102 86-87 0.0028127110440756553 1102 88-89 -0.09851718958209688 1102 90-91 -0.16649334413253314 1102 92-93 -0.36356177057308514 1102 94-95 0.002925083062731204 1102 96-97 -0.45009869640178835 1102 98-99 -0.268535040268862 1102 100-101 -0.1744055275468419 1103 1 0.20644617408733268 1103 2 0.21878295909361967 1103 3 0.2168296437202777 1103 4 0.2302128486186774 1103 5 0.2813192838652512 1103 6 0.18170824981342548 1103 7 0.15605700376943332 1103 8 0.22526722502697538 1103 9 0.33872671999282034 1103 10-11 0.2527196221459391 1103 12-13 0.28984988740423745 1103 14-15 0.3725012318385552 1103 16-17 0.387509293988451 1103 18-19 0.27534866365254373 1103 20-21 0.2407567451533481 1103 22-23 0.26380506697265815 1103 24-25 0.2799505091089358 1103 26-27 0.17497616788171655 1103 28-29 0.11574348231437881 1103 30-31 0.1973666199282107 1103 32-33 0.3687562956049817 1103 34-35 0.20974569109805685 1103 36-37 0.39334901767578145 1103 38-39 0.41515832006694353 1103 40-41 0.3843942362605972 1103 42-43 0.19590022202179824 1103 44-45 0.2632498967582677 1103 46-47 0.2784692524015 1103 48-49 0.2255716210069778 1103 50-51 0.17081368154799748 1103 52-53 0.23899190470388731 1103 54-55 0.18410774610068614 1103 56-57 0.2639781263726704 1103 58-59 0.10158335737452262 1103 60-61 -0.01354327864212479 1103 62-63 -0.02318117570906253 1103 64-65 0.11376600682464044 1103 66-67 0.3375261899635724 1103 68-69 -4.254104849046843 1103 70-71 0.36502942185035536 1103 72-73 0.6481975532318458 1103 74-75 0.44096356550609883 1103 76-77 0.2091910195665676 1103 78-79 0.04961009077688061 1103 80-81 0.12306440380247707 1103 82-83 0.09002058992426143 1103 84-85 -0.055689345886143826 1103 86-87 -0.16811968067105099 1103 88-89 -0.1506589746802085 1103 90-91 -0.39432218968395105 1103 92-93 -0.33066899976155995 1103 94-95 -0.29471334596757615 1103 96-97 -0.6894696232161976 1103 98-99 -0.34456001189808205 1103 100-101 -0.42349001469199266 1104 1 0.1411797374969801 1104 2 0.14269921234190974 1104 3 0.1522042623995965 1104 4 0.16268044278049132 1104 5 0.20485806815842977 1104 6 0.12349496187908215 1104 7 0.09870187994934554 1104 8 0.20031858134739622 1104 9 0.21163163890118852 1104 10-11 0.22715926049472301 1104 12-13 0.10139973128102753 1104 14-15 0.17754814583725675 1104 16-17 0.15532435217516394 1104 18-19 0.035658316586527405 1104 20-21 0.18122610340476797 1104 22-23 0.15919410393893685 1104 24-25 0.14235684801955983 1104 26-27 0.11842842961406319 1104 28-29 0.07603233593859215 1104 30-31 0.20600000256360573 1104 32-33 0.10889402041728147 1104 34-35 0.1898582127961035 1104 36-37 0.14899744257743208 1104 38-39 0.2483882346100259 1104 40-41 0.1655152677009326 1104 42-43 0.14859687821176948 1104 44-45 0.09541211339977451 1104 46-47 0.08979736536486627 1104 48-49 0.05673797440008599 1104 50-51 0.10071840273453603 1104 52-53 0.05106141034794831 1104 54-55 0.19366502894047954 1104 56-57 0.13481441269443195 1104 58-59 -0.09201957096286861 1104 60-61 0.0910031038186716 1104 62-63 0.17391629345146242 1104 64-65 0.08851262757875844 1104 66-67 0.2548848188525348 1104 68-69 -4.1339888993144065 1104 70-71 0.714102568657534 1104 72-73 0.9059505601444044 1104 74-75 0.4875333484742903 1104 76-77 0.3691007351552642 1104 78-79 0.1252638567358595 1104 80-81 0.0249084166826421 1104 82-83 -0.08105161449443798 1104 84-85 0.10132445098933829 1104 86-87 0.00343533735501822 1104 88-89 -0.06024071596760905 1104 90-91 -0.16256767803119843 1104 92-93 -0.28282628619630046 1104 94-95 -0.010090776405437651 1104 96-97 -0.549772856610133 1104 98-99 -0.2965744635666496 1104 100-101 -0.31591803880892044 1105 1 0.11964383012713853 1105 2 0.17559060132484916 1105 3 0.11419316553764247 1105 4 0.05673021810661538 1105 5 0.1562428787262462 1105 6 0.06982498478093646 1105 7 0.03376050539866782 1105 8 0.11022293389811466 1105 9 0.12720714660222399 1105 10-11 0.10051899513086227 1105 12-13 0.0896688048396257 1105 14-15 0.15842770735615375 1105 16-17 0.21670919145768863 1105 18-19 0.13212819260889574 1105 20-21 0.1967987458771674 1105 22-23 0.17572868918367845 1105 24-25 0.1201619692755358 1105 26-27 0.049713973430719705 1105 28-29 0.27178332047859044 1105 30-31 0.2649724629098458 1105 32-33 0.16490477694750894 1105 34-35 0.1640238382424286 1105 36-37 0.07514583120779861 1105 38-39 0.24570593740329372 1105 40-41 0.2083134811965479 1105 42-43 -0.0022929594277698584 1105 44-45 0.1093706146517377 1105 46-47 0.11942841755173106 1105 48-49 3.913494022143027E-4 1105 50-51 0.17009510751436352 1105 52-53 0.1429778200803824 1105 54-55 0.08409887324582854 1105 56-57 0.1037860465564151 1105 58-59 0.09245102717202514 1105 60-61 0.07139061381887757 1105 62-63 0.12842401596049768 1105 64-65 0.13343420021598718 1105 66-67 0.7396364029057274 1105 68-69 1.9004743391155614 1105 70-71 2.0833982667445845 1105 72-73 1.1007173168004734 1105 74-75 0.5599765033369657 1105 76-77 0.42048683164268397 1105 78-79 0.35540638656669543 1105 80-81 0.2713906462724758 1105 82-83 0.3135153689329293 1105 84-85 0.15005908737122553 1105 86-87 0.12893647146119847 1105 88-89 0.23434214794264818 1105 90-91 0.20446292885293715 1105 92-93 0.2032549137589541 1105 94-95 0.5364616044684674 1105 96-97 0.08796829701206832 1105 98-99 0.21749212359772585 1105 100-101 0.23364754508846985 1106 1 0.5114332226282876 1106 2 0.40322974677156154 1106 3 0.4082486062281774 1106 4 0.33945341173698296 1106 5 0.44533322101055717 1106 6 0.4165409614985549 1106 7 0.41457696800965493 1106 8 0.38241148561491656 1106 9 0.533901769358998 1106 10-11 0.5385093664242007 1106 12-13 0.5090056029109533 1106 14-15 0.650471143847227 1106 16-17 0.5856116023281501 1106 18-19 0.5959308407420494 1106 20-21 0.6080331073488381 1106 22-23 0.617637126991454 1106 24-25 0.7009574154621916 1106 26-27 0.8088929445238975 1106 28-29 0.7277673811128906 1106 30-31 0.7297870200316865 1106 32-33 0.7707771161806107 1106 34-35 0.7505567045865646 1106 36-37 0.7972247950089226 1106 38-39 0.8477380345781498 1106 40-41 0.7338321331139284 1106 42-43 0.7855177692021087 1106 44-45 0.8422921109829389 1106 46-47 0.9803841362640853 1106 48-49 1.0270187132325006 1106 50-51 1.044891606432806 1106 52-53 1.0345851339234287 1106 54-55 1.0229426387394724 1106 56-57 1.0319867126496902 1106 58-59 1.0644136523272252 1106 60-61 1.0881556384128714 1106 62-63 1.1194644913647593 1106 64-65 1.249617643031094 1106 66-67 1.654938779895165 1106 68-69 3.1005021699562754 1106 70-71 3.45866012024673 1106 72-73 2.3055531960400977 1106 74-75 1.865792544027311 1106 76-77 1.3928384517064885 1106 78-79 1.261043723964054 1106 80-81 1.1539578088736882 1106 82-83 1.147333661564982 1106 84-85 1.2918909744455185 1106 86-87 1.279982627668641 1106 88-89 1.4078881696131305 1106 90-91 1.283758407360299 1106 92-93 1.365555428884253 1106 94-95 1.6825674043328789 1106 96-97 1.5528210658145234 1106 98-99 1.7143340874106272 1106 100-101 1.8951145679304595 1107 1 0.09491116419782131 1107 2 0.05048392974032723 1107 3 0.06215869244582706 1107 4 0.026007009484686705 1107 5 -0.0053151296027920125 1107 6 0.14739024480002882 1107 7 0.04306831187984983 1107 8 0.14038101797262925 1107 9 -0.01704250099837168 1107 10-11 0.020511845786195693 1107 12-13 0.03669134505221194 1107 14-15 0.11219478408008854 1107 16-17 -0.025945209171609918 1107 18-19 -0.017373392755807515 1107 20-21 0.09279858617880876 1107 22-23 0.08413716644742664 1107 24-25 0.10017195536743628 1107 26-27 0.01658991252384112 1107 28-29 0.1570367711745675 1107 30-31 0.03573195223106751 1107 32-33 0.10138011788867374 1107 34-35 0.12098630368849683 1107 36-37 -0.02963208547513574 1107 38-39 0.17119651544709313 1107 40-41 0.03450514370594959 1107 42-43 0.029993488509823862 1107 44-45 0.023576809959372724 1107 46-47 0.06028093982174454 1107 48-49 0.16547211573681153 1107 50-51 0.11167999263289374 1107 52-53 0.17287670332901683 1107 54-55 0.008877668764192492 1107 56-57 0.09258735336366186 1107 58-59 0.24779794961646928 1107 60-61 0.369145244947795 1107 62-63 0.2798719251232811 1107 64-65 0.02688365642575974 1107 66-67 0.6985568728737839 1107 68-69 2.1554673850676522 1107 70-71 2.409185584617994 1107 72-73 1.4439474782342572 1107 74-75 0.8274885969033932 1107 76-77 0.3926986057629449 1107 78-79 0.32998233302670954 1107 80-81 0.35699496366580163 1107 82-83 0.4445156146607516 1107 84-85 0.40657053472787297 1107 86-87 0.31690991159631565 1107 88-89 0.548449761648623 1107 90-91 0.5174750575233453 1107 92-93 0.39673016026549135 1107 94-95 0.8371667926381328 1107 96-97 0.4275509515449549 1107 98-99 0.5496553862501123 1107 100-101 0.6971261858032456 1108 1 -0.19740801290999954 1108 2 -0.25184900033038815 1108 3 -0.16304508981600563 1108 4 -0.07090877036544896 1108 5 -0.06738128568906632 1108 6 0.010264288939552557 1108 7 -0.05883784286659477 1108 8 -0.11208116630525211 1108 9 -0.1585467953511852 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2316 64-65 -0.6503674401509656 2316 66-67 -0.05409457167313647 2316 68-69 1.2854438502496421 2316 70-71 1.5573931713094957 2316 72-73 0.5924054770503631 2316 74-75 0.16104213180906513 2316 76-77 -0.31627408974190985 2316 78-79 -0.4323433416096236 2316 80-81 -0.513178806892558 2316 82-83 -0.41927206999944744 2316 84-85 -0.4129722053505702 2316 86-87 -0.38420072557854823 2316 88-89 -0.3977006450317404 2316 90-91 -0.5486497762080802 2316 92-93 -0.5564894291370592 2316 94-95 -0.1201601150270406 2316 96-97 -0.18723760304073522 2316 98-99 -0.43509617631692876 2316 100-101 -0.6667579265567092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 29.0 4 72.0 5 151.0 6 382.0 7 1000.0 8 2264.0 9 4060.0 10 6366.0 11 9739.0 12 13240.0 13 17122.0 14 21179.0 15 25817.0 16 31227.0 17 35731.0 18 33879.0 19 30832.0 20 30902.0 21 32329.0 22 35391.0 23 40687.0 24 47870.0 25 59334.0 26 73277.0 27 90819.0 28 111115.0 29 134473.0 30 159907.0 31 190351.0 32 226844.0 33 274745.0 34 339538.0 35 430219.0 36 537800.0 37 576464.0 38 318974.0 39 24951.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.991116823224786 19.253334341353394 14.583555412752712 24.17199342266911 2 16.027849627334326 20.175683085998376 39.331474877071734 24.464992409595563 3 19.52375457535837 23.639737894448295 29.669284228517988 27.167223301675346 4 13.464849625480904 16.74129334431822 36.269825073253564 33.52403195694731 5 13.984994041455344 37.08104376569945 34.26969406085526 14.664268131989951 6 33.2332096273957 36.09074120264343 16.713100483990033 13.962948685970838 7 29.15554950882948 31.945634893640605 20.287924939973646 18.61089065755627 8 26.322885094568278 35.77762157068749 19.075027273254072 18.824466061490167 9 26.014426480629062 16.52509769241816 19.59335767140579 37.86711815554699 10-11 25.260324154907043 26.15176274662454 27.2730525132965 21.314860585171918 12-13 25.976407690427784 23.22221214434543 27.61750174473242 23.18387842049437 14-15 22.617337979234534 24.867526309557103 25.52702256688561 26.988113144322757 16-17 22.626887685083776 27.944466237902425 26.315524928818533 23.113121148195262 18-19 22.578525556235814 26.71404528493937 28.26102456734415 22.44640459148067 20-21 23.134647992360968 26.035564827202208 28.377436641648334 22.452350538788487 22-23 22.855402623775223 25.907273455628367 28.280222922634657 22.95710099796175 24-25 22.682115221697682 26.10152837396505 28.36790572140322 22.84845068293405 26-27 22.56525441664063 26.37337871748617 28.17564775716287 22.88571910871033 28-29 22.575904086782657 26.568256990018867 28.11923041262879 22.736608510569685 30-31 22.740271511453226 26.30700710681512 28.00192236105241 22.950799020679238 32-33 22.444107669031233 26.297809406112094 28.092876948058905 23.165205976797772 34-35 22.986234228623097 26.44440068799252 27.883765394701882 22.685599688682505 36-37 23.040869276621674 26.13936701748298 27.975604429303942 22.84415927659141 38-39 22.709521008019806 26.406189597073 27.918196611472233 22.966092783434963 40-41 23.071257458300476 26.238212171704266 27.914049005690238 22.77648136430502 42-43 22.790698554167644 26.203031884979026 27.853822893732712 23.152446667120614 44-45 22.79899889759707 26.392456704237638 27.997125657416344 22.81141874074895 46-47 22.980392413972055 26.565761201353922 27.474538279766364 22.97930810490766 48-49 22.80327972819475 26.648062661362932 27.417649664297016 23.131007946145303 50-51 22.83429929081305 26.789231978067757 27.378615658194285 22.997853072924915 52-53 23.084250013299222 26.498406488306642 27.34107301916576 23.076270479228377 54-55 22.763359567613353 26.62736146333839 27.475219445298755 23.134059523749503 56-57 22.670538444032413 26.76731960485491 27.364959289127967 23.197182661984712 58-59 22.77158777882713 26.506455192627705 27.318847408700524 23.40310961984464 60-61 22.56829907833457 26.652253190213166 27.506277496923143 23.273170234529125 62-63 22.620942805827493 26.609048669386315 27.551914965289242 23.21809355949695 64-65 22.33789067076586 26.61287945232332 27.427284209698833 23.62194566721199 66-67 22.168390836716263 26.718696009958105 27.53529194408494 23.577621209240693 68-69 22.238608898888682 26.329363347890368 27.980935469714158 23.451092283506792 70-71 22.15738223658371 26.31208977634062 27.69104065078894 23.83948733628673 72-73 22.278569696180444 26.55119388418752 27.41315123794929 23.757085181682747 74-75 22.473723651353673 26.699928011039397 27.006029154205947 23.820319183400983 76-77 22.363519723300072 26.483045160945622 27.153347916162886 24.000087199591423 78-79 22.503518601851493 26.55889305516432 27.01222956653546 23.925358776448725 80-81 22.50374712871302 26.564515994929756 26.874515468353927 24.057221408003297 82-83 22.497586347291872 26.620536002225197 26.885710268958512 23.996167381524423 84-85 22.447953802779995 26.572018130191704 26.85372140073115 24.126306666297143 86-87 22.336613178074945 26.484694848715918 26.897187594126994 24.281504379082143 88-89 22.357820361942725 26.533648469255226 26.696041224656287 24.41248994414576 90-91 22.283129297291303 26.494884192014755 26.767781873335565 24.454204637358373 92-93 22.309508599350742 26.276057784452295 26.86973504478405 24.544698571412916 94-95 21.985242040668854 26.123027706346562 27.150351312147787 24.7413789408368 96-97 22.202382437304976 25.768592175749923 27.119936035592673 24.909089351352428 98-99 21.992497733973963 25.960393115514286 26.886786390790647 25.160322759721105 100-101 21.90458324603623 25.855271206015328 27.0350199238975 25.20512562405094 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 360.0 1 250.5 2 183.0 3 240.5 4 241.0 5 242.5 6 407.5 7 899.0 8 1517.0 9 1859.5 10 1811.5 11 1749.0 12 1916.0 13 2175.0 14 2416.5 15 2728.5 16 3063.5 17 3367.5 18 3794.5 19 4340.5 20 5117.5 21 6103.5 22 7481.5 23 9370.0 24 11825.0 25 14879.0 26 19548.5 27 25296.5 28 29648.5 29 34503.0 30 40172.0 31 47766.5 32 58231.0 33 64324.5 34 68189.5 35 74457.0 36 82744.5 37 91565.5 38 97248.0 39 101975.5 40 108519.5 41 115129.0 42 120942.0 43 127294.5 44 132933.5 45 138191.0 46 142506.5 47 146999.0 48 149998.0 49 149670.5 50 149743.0 51 149773.5 52 148972.0 53 149213.5 54 149191.0 55 142690.0 56 131466.0 57 120288.0 58 111064.5 59 99021.5 60 82571.0 61 67687.5 62 54881.0 63 42101.0 64 30733.5 65 22612.5 66 16525.0 67 12094.5 68 8769.0 69 6380.5 70 4644.0 71 3370.0 72 2536.5 73 2104.0 74 1607.5 75 1093.0 76 756.5 77 458.0 78 285.0 79 186.5 80 106.0 81 58.5 82 37.0 83 21.5 84 14.5 85 10.5 86 3.0 87 1.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02305314316379825 2 0.023229506007674303 3 5.0389383964586344E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.2675222784063851E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.298672995573293E-5 26-27 2.519469198229317E-4 28-29 5.0389383964586344E-5 30-31 3.149336497786647E-4 32-33 0.0018896018986719878 34-35 0.0038169958353174156 36-37 0.09730190043561622 38-39 0.08901284677344178 40-41 0.09767982081535062 42-43 0.09825929873094337 44-45 0.09225036469316644 46-47 0.08644298819124788 48-49 0.08708545283679635 50-51 0.08621623596340723 52-53 0.06784930550831551 54-55 0.057191950799805505 56-57 0.03101466583020289 58-59 0.012647735375111172 60-61 0.008830739539793755 62-63 0.0019021992446631346 64-65 0.004509849864830478 66-67 0.14324442126532783 68-69 0.39492679682244547 70-71 0.3778447956584507 72-73 0.17059325941210707 74-75 0.028230652366159494 76-77 0.029767528577079382 78-79 0.004635823324741943 80-81 0.0012849292910969516 82-83 5.0389383964586344E-5 84-85 5.038938396458634E-4 86-87 0.001272331945105805 88-89 2.2675222784063851E-4 90-91 5.038938396458634E-4 92-93 0.0024816771602558774 94-95 0.07455109357560549 96-97 0.015948240024791577 98-99 0.017359142775799995 100-101 0.009158270535563569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3969090.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.485567568676196 #Duplication Level Percentage of deduplicated Percentage of total 1 69.58043966309889 33.040666690802794 2 15.157398333749178 14.395153254851733 3 6.053935784244171 8.624237302174599 4 2.991854000939252 5.682795412688603 5 1.715242169701901 4.072462397301119 6 1.0506631597313343 2.993480187800466 7 0.693264884814628 2.304405357159888 8 0.49528015562083827 1.881492743612622 9 0.3487489593499079 1.4904488046344007 >10 1.7395318584721975 14.645741568508196 >50 0.11493076520353962 3.7558610203753076 >100 0.05419473761624765 4.433934097659245 >500 0.0026030688270601305 0.8185637679093425 >1k 0.0018062109291359508 1.4387993811793984 >5k 1.0624770171387847E-4 0.421958013342234 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9256 0.23320206898810558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7471 0.18822954379971227 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4981 0.12549476076380228 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.0389383964586344E-5 2 0.0 0.0 0.0 0.0 5.0389383964586344E-5 3 0.0 0.0 0.0 0.0 5.0389383964586344E-5 4 0.0 0.0 0.0 0.0 5.0389383964586344E-5 5 0.0 0.0 0.0 0.0 5.0389383964586344E-5 6 2.5194691982293172E-5 0.0 0.0 0.0 1.0077876792917269E-4 7 2.5194691982293172E-5 0.0 0.0 5.0389383964586344E-5 1.0077876792917269E-4 8 2.5194691982293172E-5 0.0 0.0 5.0389383964586344E-5 1.0077876792917269E-4 9 2.5194691982293172E-5 0.0 0.0 1.0077876792917269E-4 1.0077876792917269E-4 10-11 2.5194691982293172E-5 0.0 0.0 1.0077876792917269E-4 1.2597345991146585E-4 12-13 3.779203797343976E-5 0.0 0.0 1.0077876792917269E-4 2.8973895779637145E-4 14-15 5.0389383964586344E-5 0.0 0.0 1.0077876792917269E-4 3.779203797343976E-4 16-17 7.558407594687951E-5 0.0 0.0 1.5116815189375902E-4 4.535044556812771E-4 18-19 7.558407594687951E-5 1.2597345991146586E-5 0.0 1.7636284387605218E-4 4.7869914766357025E-4 20-21 1.0077876792917269E-4 2.5194691982293172E-5 0.0 2.0155753585834538E-4 5.1649118563701E-4 22-23 1.0077876792917269E-4 2.5194691982293172E-5 0.0 3.65323033743251E-4 5.668805696015963E-4 24-25 1.0077876792917269E-4 2.5194691982293172E-5 0.0 8.062301434333815E-4 6.298672995573292E-4 26-27 1.0077876792917269E-4 2.5194691982293172E-5 0.0 0.0013227213290703914 7.054513755042087E-4 28-29 1.1337611392031927E-4 2.5194691982293172E-5 0.0 0.005492442852139911 7.054513755042087E-4 30-31 1.3857080590261244E-4 2.5194691982293172E-5 0.0 0.01939991282636574 7.054513755042087E-4 32-33 1.5116815189375902E-4 2.5194691982293172E-5 0.0 0.05101925126414367 7.432434134776485E-4 34-35 1.5116815189375902E-4 2.5194691982293172E-5 0.0 0.09669722782804119 7.810354514510883E-4 36-37 1.7636284387605218E-4 2.5194691982293172E-5 0.0 0.17691712709966265 7.810354514510883E-4 38-39 1.7636284387605218E-4 2.5194691982293172E-5 0.0 0.3007364408466424 8.062301434333815E-4 40-41 1.7636284387605218E-4 2.5194691982293172E-5 0.0 0.441637755757617 8.062301434333815E-4 42-43 2.0155753585834538E-4 2.5194691982293172E-5 0.0 0.5856884071663782 8.062301434333815E-4 44-45 2.2675222784063854E-4 2.5194691982293172E-5 0.0 0.7555384231650075 8.944115653714076E-4 46-47 2.519469198229317E-4 2.5194691982293172E-5 0.0 0.9605854238629006 0.0010581770632563132 48-49 2.519469198229317E-4 2.5194691982293172E-5 0.0 1.1776754873283297 0.0011463584851943392 50-51 2.519469198229317E-4 2.5194691982293172E-5 0.0 1.412666379447178 0.0011589558311854858 52-53 2.519469198229317E-4 2.5194691982293172E-5 0.0 1.6390784789460557 0.001247137253123512 54-55 2.519469198229317E-4 2.5194691982293172E-5 0.0 1.8761731278454254 0.0013101239830792448 56-57 2.519469198229317E-4 2.5194691982293172E-5 0.0 2.1438667301572902 0.001335318675061538 58-59 2.519469198229317E-4 2.5194691982293172E-5 0.0 2.421222496844365 0.0013731107130349777 60-61 2.519469198229317E-4 2.5194691982293172E-5 0.0 2.7103693793791526 0.0013857080590261243 62-63 2.645442658140783E-4 2.5194691982293172E-5 0.0 2.9909752613319425 0.0013857080590261243 64-65 3.0233630378751804E-4 5.0389383964586344E-5 0.0 3.275120997508245 0.0014360974429907106 66-67 3.0233630378751804E-4 7.558407594687951E-5 0.0 3.587900501122424 0.001486486826955297 68-69 3.0233630378751804E-4 7.558407594687951E-5 0.0 3.942087481009501 0.0015116815189375901 70-71 3.149336497786646E-4 7.558407594687951E-5 0.0 4.32870002947779 0.0015620709029021767 72-73 3.275309957698112E-4 7.558407594687951E-5 0.0 4.737269752008647 0.0016502523248402028 74-75 3.275309957698112E-4 7.558407594687951E-5 0.0 5.1448946735901675 0.0017132390547959357 76-77 3.275309957698112E-4 7.558407594687951E-5 0.0 5.58262725209053 0.0017762257847516686 78-79 3.275309957698112E-4 7.558407594687951E-5 0.0 6.071202719011159 0.0018140178227251083 80-81 3.6532303374325095E-4 7.558407594687951E-5 0.0 6.583473793741135 0.0018770045526808412 82-83 4.0311507171669075E-4 7.558407594687951E-5 0.0 7.107044687825169 0.0020155753585834536 84-85 4.0311507171669075E-4 7.558407594687951E-5 0.0 7.607549841399415 0.002091159434530333 86-87 4.0311507171669075E-4 7.558407594687951E-5 0.0 8.116709371669577 0.002179340856468359 88-89 4.0311507171669075E-4 7.558407594687951E-5 0.0 8.660083293651693 0.0022675222784063853 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5205 0.0 55.076317 1 GTATCAA 9550 0.0 43.43669 1 ATCAACG 12385 0.0 33.13509 3 TCAACGC 12475 0.0 32.93407 4 TATCAAC 12790 0.0 32.315742 2 CAACGCA 12815 0.0 32.134327 5 AACGCAG 13510 0.0 30.481228 6 ACGCAGA 15365 0.0 26.646875 7 CGCAGAG 15755 0.0 25.89692 8 TGGTATC 2350 0.0 23.423763 2 GTACATG 11800 0.0 22.926695 1 TACATGG 11435 0.0 22.907066 2 GCAGAGT 17510 0.0 22.542664 9 GTGGTAT 2630 0.0 22.377657 1 TAAGGTG 2050 0.0 21.291292 5 AGAGTAC 15700 0.0 21.062078 10-11 ACATGGG 11855 0.0 20.809895 3 GAGTACT 11095 0.0 20.161482 12-13 CAGAGTA 16740 0.0 20.0228 10-11 GTAAGGT 1870 0.0 19.281466 4 >>END_MODULE