FastQCFastQC Report
Fri 27 May 2016
SRR939881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820765
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68980.2445435901253738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51280.18179465499607375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.11943568500034564No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29510.10461700992461265No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28410.10071735858889344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55350.037.9703031
GTATCAA92600.036.272731
TATCAAC110450.031.0543772
ATCAACG114250.029.8969273
TCAACGC114450.029.8468024
CAACGCA115750.029.5115915
AACGCAG120600.028.3641036
TACATGG101700.026.4025422
GTACATG101350.026.0260941
ACATGGG106200.024.4797133
ACGCAGA139000.024.3363727
CGCAGAG140450.023.9825088
TAAGGTG12750.021.9544685
GCAGAGT150000.021.127269
GAGTACT77850.020.62694512-13
AGAGTAC118150.020.43744510-11
GTAAGGT13300.020.3331344
GTACTTT79150.020.1715114-15
CATGGGG73750.019.685234
GTATAGG5600.018.6377141
Produced by FastQC (version 0.11.5)