##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939881_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2820765 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.57208168706007 31.0 28.0 33.0 16.0 34.0 2 29.382852878563085 31.0 30.0 33.0 23.0 34.0 3 29.647445285232905 31.0 30.0 33.0 25.0 34.0 4 33.41194675912386 35.0 33.0 37.0 28.0 37.0 5 33.33369103771495 35.0 33.0 37.0 28.0 37.0 6 33.34622983481432 35.0 33.0 37.0 26.0 37.0 7 33.23406026379369 35.0 33.0 37.0 26.0 37.0 8 33.079397255709004 35.0 33.0 37.0 26.0 37.0 9 34.31530028201569 37.0 33.0 39.0 26.0 39.0 10-11 34.116942389741794 37.0 33.0 39.0 25.5 39.0 12-13 33.962249779758324 37.0 33.0 39.0 25.0 39.0 14-15 34.577218768667365 38.0 33.0 40.0 25.0 41.0 16-17 34.369148617484974 38.0 33.0 40.0 24.5 41.0 18-19 34.15033563590019 38.0 32.0 40.0 24.0 41.0 20-21 34.30142727238887 38.0 33.0 40.0 24.0 41.0 22-23 34.17559722273923 38.0 32.0 40.0 23.5 41.0 24-25 33.93066047685646 38.0 32.0 40.0 21.0 41.0 26-27 33.78160250853935 38.0 32.0 40.0 20.0 41.0 28-29 33.83044262815229 38.0 32.0 40.0 21.0 41.0 30-31 33.592539789737884 38.0 32.0 40.0 18.5 41.0 32-33 33.20951550377291 37.0 31.0 40.0 17.0 41.0 34-35 33.00143294460899 37.0 31.0 40.0 16.0 41.0 36-37 32.766524861163546 37.0 30.5 40.0 15.5 41.0 38-39 32.491432111501666 37.0 30.0 40.0 15.0 41.0 40-41 32.22068800484975 37.0 30.0 40.0 14.0 41.0 42-43 31.779847665438275 36.0 30.0 39.0 9.0 41.0 44-45 31.407101265082346 36.0 29.5 39.0 8.5 40.5 46-47 30.983398475236328 35.5 28.5 39.0 7.5 40.0 48-49 30.568951153321883 35.0 28.0 39.0 4.5 40.0 50-51 29.90407300856328 34.5 27.0 38.5 2.0 40.0 52-53 30.030565821683126 35.0 27.5 38.5 2.0 40.0 54-55 30.07928345679275 35.0 28.0 39.0 2.0 40.0 56-57 29.707119168027113 35.0 27.0 38.5 2.0 40.0 58-59 29.246295774373266 34.0 26.0 38.0 2.0 40.0 60-61 28.77184770797993 34.0 26.0 38.0 2.0 40.0 62-63 28.260919112368452 34.0 25.0 37.5 2.0 40.0 64-65 27.662990890768995 33.0 23.5 37.0 2.0 39.0 66-67 27.018949469381532 32.5 22.0 36.0 2.0 39.0 68-69 26.44534372767671 32.0 21.5 36.0 2.0 39.0 70-71 25.82368453238749 31.5 19.5 35.0 2.0 38.0 72-73 25.13806059703662 31.0 18.0 35.0 2.0 37.0 74-75 24.759072627460988 31.0 15.5 35.0 2.0 37.0 76-77 24.18907087261789 31.0 9.5 34.0 2.0 36.0 78-79 23.52015144827733 30.0 6.5 34.0 2.0 36.0 80-81 22.78639340746216 29.5 2.0 34.0 2.0 35.0 82-83 22.043894475434854 29.0 2.0 34.0 2.0 35.0 84-85 21.27863912803796 28.5 2.0 34.0 2.0 35.0 86-87 20.399395376786085 27.0 2.0 33.0 2.0 35.0 88-89 19.441549898697694 25.5 2.0 33.0 2.0 34.5 90-91 18.066694495996654 22.5 2.0 32.0 2.0 34.0 92-93 16.592961483852783 17.0 2.0 31.0 2.0 34.0 94-95 16.005225001019227 12.5 2.0 31.0 2.0 34.0 96-97 15.562695403551874 4.5 2.0 31.0 2.0 34.0 98-99 14.63519045365353 2.0 2.0 31.0 2.0 34.0 100-101 13.107717055479629 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.78422055237575 1101 2 1.1232282600500518 1101 3 0.7724677728065394 1101 4 0.7667628945250939 1101 5 0.9799237246618588 1101 6 0.9787747411687633 1101 7 0.9629019398716636 1101 8 0.9978439493159996 1101 9 1.110267649141555 1101 10-11 1.118468145571839 1101 12-13 1.074577667977536 1101 14-15 1.2680598788624309 1101 16-17 0.9536037418776999 1101 18-19 1.1785539244753664 1101 20-21 1.081125998842417 1101 22-23 1.1007802195591267 1101 24-25 1.1176721946692894 1101 26-27 1.223186983196804 1101 28-29 1.0579678644596058 1101 30-31 0.8413460124206082 1101 32-33 1.1733913918741408 1101 34-35 0.7989937279207382 1101 36-37 0.8806089144905371 1101 38-39 0.9162331991370891 1101 40-41 1.2880911940480715 1101 42-43 0.8178597589634791 1101 44-45 0.6047846127585892 1101 46-47 0.8183424929653995 1101 48-49 0.9390941139826232 1101 50-51 0.7464419921476662 1101 52-53 0.4445755178976256 1101 54-55 0.38487950960860573 1101 56-57 0.5214247932283946 1101 58-59 0.3709601107553233 1101 60-61 0.45083048493912514 1101 62-63 0.06641542885610363 1101 64-65 0.34013801731599713 1101 66-67 0.40833306121018964 1101 68-69 0.07850019664574148 1101 70-71 0.16730895249133937 1101 72-73 -0.05165448317167787 1101 74-75 -0.15586325696277825 1101 76-77 -0.3102566897594805 1101 78-79 -0.6448831047118233 1101 80-81 -0.8160552171748812 1101 82-83 -0.5334709099606627 1101 84-85 -0.47529072676713824 1101 86-87 -0.9954857070257681 1101 88-89 -4.873594415909558 1101 90-91 -8.233498989995839 1101 92-93 -9.974613278852958 1101 94-95 -8.041986612534938 1101 96-97 -5.196462494950158 1101 98-99 -3.9814813272455734 1101 100-101 -3.045657969218107 1102 1 1.7742179900272284 1102 2 1.1078588704766936 1102 3 0.9430884488434081 1102 4 0.9623135761333117 1102 5 1.0177289438601917 1102 6 0.9759737886333326 1102 7 0.9985080058204048 1102 8 1.0902565767575751 1102 9 1.3927360135404285 1102 10-11 1.3452371651326658 1102 12-13 1.210678925408935 1102 14-15 1.4110401017632412 1102 16-17 1.3153074450590125 1102 18-19 1.4143987119767516 1102 20-21 1.2771192144957766 1102 22-23 1.264511939318126 1102 24-25 1.4150450822009972 1102 26-27 1.4696408338894642 1102 28-29 1.3739759605406263 1102 30-31 1.4453761870129185 1102 32-33 1.6944537791836964 1102 34-35 1.7241179077929019 1102 36-37 1.6352193787457807 1102 38-39 1.403825202729081 1102 40-41 1.3140855145673171 1102 42-43 1.4798613057757137 1102 44-45 1.4597862465496227 1102 46-47 1.3981661140575241 1102 48-49 1.240773627653578 1102 50-51 1.5388088969481934 1102 52-53 1.5632010882552478 1102 54-55 1.4376010058320787 1102 56-57 1.5066395721024186 1102 58-59 1.3443046337941276 1102 60-61 1.1402673794667457 1102 62-63 1.1939815973928134 1102 64-65 1.1877546805740344 1102 66-67 1.1510180263363914 1102 68-69 1.1389451755004885 1102 70-71 1.0281217106361957 1102 72-73 1.0005151678082456 1102 74-75 0.8801500813542091 1102 76-77 0.7176570413725862 1102 78-79 0.7747044041917519 1102 80-81 0.773352527699835 1102 82-83 0.3853132204041074 1102 84-85 0.28434276336311726 1102 86-87 0.4380048933865588 1102 88-89 0.06588244185188685 1102 90-91 -5.394485000513221 1102 92-93 -9.781571465556485 1102 94-95 -7.944072575801673 1102 96-97 -4.711643234552078 1102 98-99 -3.295612326726051 1102 100-101 -2.5687872784888732 1103 1 2.0700715860228094 1103 2 1.4831997245349768 1103 3 1.2029197524400956 1103 4 1.0851165063716053 1103 5 1.2072253350175117 1103 6 0.9909009111480174 1103 7 0.8884696688241434 1103 8 0.962043198122835 1103 9 1.0737389148105478 1103 10-11 1.1293743653244945 1103 12-13 1.1499168031050857 1103 14-15 1.1812974601642665 1103 16-17 1.1395587578595112 1103 18-19 1.0512360813416777 1103 20-21 0.8574053891592044 1103 22-23 1.0443803934207239 1103 24-25 1.0107555005030164 1103 26-27 1.0208256479653741 1103 28-29 0.9128608900963613 1103 30-31 0.9371598067933107 1103 32-33 1.0631453762913878 1103 34-35 1.0349816544360877 1103 36-37 1.0392579771366073 1103 38-39 0.8517566759703143 1103 40-41 0.8173443687843189 1103 42-43 0.9366890339243099 1103 44-45 0.7895844672331656 1103 46-47 0.8804417685064827 1103 48-49 0.7870693419089818 1103 50-51 0.5099391377062048 1103 52-53 0.654951431302873 1103 54-55 0.7113441707440238 1103 56-57 0.7613527282374761 1103 58-59 0.9916823198655429 1103 60-61 0.9640317204813584 1103 62-63 0.7770369698237545 1103 64-65 0.8432380863409001 1103 66-67 0.6594413220922917 1103 68-69 0.5947653690424701 1103 70-71 0.4510616503897289 1103 72-73 0.4408303813647194 1103 74-75 0.3663268794174783 1103 76-77 0.39545425786097965 1103 78-79 -0.05905958847139203 1103 80-81 -0.06907349934114748 1103 82-83 -0.5919436191365577 1103 84-85 -0.746041539029008 1103 86-87 -0.7547717638169971 1103 88-89 -1.60682419408651 1103 90-91 -6.5593702291797715 1103 92-93 -9.540650604986912 1103 94-95 -8.454385678295843 1103 96-97 -5.6738784645151235 1103 98-99 -4.2857423246857085 1103 100-101 -3.3815826733347905 1104 1 1.9205431436132017 1104 2 1.1982880686624924 1104 3 1.0374316530954957 1104 4 0.7098567588650866 1104 5 0.8317566827254623 1104 6 0.8733239484114677 1104 7 0.8326892088462401 1104 8 0.9544299329917649 1104 9 0.9135442229289623 1104 10-11 1.0027238215264305 1104 12-13 0.9854569718521944 1104 14-15 0.9492330658405734 1104 16-17 1.0272123121000476 1104 18-19 0.9527236402085535 1104 20-21 1.0643150878751584 1104 22-23 1.0555455587091416 1104 24-25 1.0074982806060575 1104 26-27 1.2027728965095008 1104 28-29 0.9071504800895909 1104 30-31 1.069853025273595 1104 32-33 0.9919396843968897 1104 34-35 1.2310640440854854 1104 36-37 1.3308299718129888 1104 38-39 1.2252648859745037 1104 40-41 0.9937888439214291 1104 42-43 1.2173285177588653 1104 44-45 1.12373280208217 1104 46-47 0.8632458610111797 1104 48-49 0.9436765091416426 1104 50-51 0.9865970254528555 1104 52-53 0.9380464110395685 1104 54-55 0.9251623691639104 1104 56-57 0.9597251731889926 1104 58-59 1.0174648250812481 1104 60-61 0.789693183128449 1104 62-63 0.8007567132167388 1104 64-65 0.86815424773161 1104 66-67 0.7712657941119652 1104 68-69 0.7825032691666145 1104 70-71 0.7725721103860757 1104 72-73 0.5581734791137158 1104 74-75 0.5905863396222699 1104 76-77 0.5546637999675141 1104 78-79 0.39352340621790916 1104 80-81 0.21903836571281943 1104 82-83 -0.04880126459055134 1104 84-85 -0.2675466841740253 1104 86-87 -0.35538403238462024 1104 88-89 -0.8985645116033361 1104 90-91 -3.6450731292209078 1104 92-93 -7.579430803949121 1104 94-95 -5.541388526410534 1104 96-97 -3.4590604168940544 1104 98-99 -2.521025374529559 1104 100-101 -2.0876705876626147 1105 1 2.0564020912558156 1105 2 1.2691848631708673 1105 3 1.0109273551012272 1105 4 0.9828856956286458 1105 5 0.946029892660647 1105 6 0.8642816886318059 1105 7 0.8339351291992756 1105 8 0.880883283282131 1105 9 1.0475826520541958 1105 10-11 0.9372794847659449 1105 12-13 0.8423264844397238 1105 14-15 1.2098449362725106 1105 16-17 0.9458410718873651 1105 18-19 0.9240607684435389 1105 20-21 0.9570395259181552 1105 22-23 1.0143119156035567 1105 24-25 1.0070247159522694 1105 26-27 0.8850004795197819 1105 28-29 0.970262427134621 1105 30-31 1.2646260015388577 1105 32-33 1.3019789677580604 1105 34-35 1.3106267365758697 1105 36-37 1.353980053022866 1105 38-39 1.3847814765388335 1105 40-41 1.2607169846749926 1105 42-43 1.4340353424527947 1105 44-45 1.4203513876513334 1105 46-47 1.2624670889657637 1105 48-49 1.0414276276927374 1105 50-51 1.1837627242408324 1105 52-53 1.0627557601347704 1105 54-55 0.9980788762199744 1105 56-57 1.284917567807689 1105 58-59 1.2143283810011418 1105 60-61 1.0463307495903038 1105 62-63 0.920801282881019 1105 64-65 0.9697949170235525 1105 66-67 1.0483638435427274 1105 68-69 0.9274241086928399 1105 70-71 0.6674608681814576 1105 72-73 0.6648375728574933 1105 74-75 0.6063528344202425 1105 76-77 0.5499664599739482 1105 78-79 0.4684663294669136 1105 80-81 0.4310794533122255 1105 82-83 -0.006851289106741376 1105 84-85 -0.004515276638361598 1105 86-87 0.24893665851221414 1105 88-89 -0.18682460920841137 1105 90-91 -3.064246799600756 1105 92-93 -7.570304269772022 1105 94-95 -5.3846917763377515 1105 96-97 -2.789018145622432 1105 98-99 -1.8377247294473538 1105 100-101 -1.3577441745341954 1106 1 1.8820412681813572 1106 2 1.1380784083498448 1106 3 0.956631797865672 1106 4 0.9116518461626697 1106 5 0.9951436419904667 1106 6 0.9823099571867075 1106 7 1.156523087970882 1106 8 -0.0854549282669268 1106 9 -0.2690817590599224 1106 10-11 -0.3813116737791873 1106 12-13 -0.06500005579155754 1106 14-15 0.8600119119758105 1106 16-17 0.974235172917453 1106 18-19 1.1529659076811285 1106 20-21 0.8933993052162279 1106 22-23 0.9844010202995648 1106 24-25 0.9030044657570429 1106 26-27 0.9737772130026556 1106 28-29 0.7948581517477322 1106 30-31 0.9965235071570007 1106 32-33 0.9756506806402214 1106 34-35 1.0841265478353748 1106 36-37 1.041892080251273 1106 38-39 0.92943843551474 1106 40-41 0.8856261792405107 1106 42-43 1.0740122113725263 1106 44-45 0.8821012997625637 1106 46-47 0.8865525531481069 1106 48-49 0.6066812814286067 1106 50-51 0.6812259405861774 1106 52-53 0.9119428408482904 1106 54-55 0.9717701890760004 1106 56-57 0.944486503197254 1106 58-59 0.8709696351090983 1106 60-61 0.5488640980643567 1106 62-63 0.648266044409489 1106 64-65 0.45007931368139253 1106 66-67 0.21047973849931267 1106 68-69 0.4819563045017645 1106 70-71 0.7383488662567181 1106 72-73 0.6864364329479002 1106 74-75 0.5844283451343735 1106 76-77 0.5244912902371404 1106 78-79 0.2836158907489832 1106 80-81 0.17996360294817393 1106 82-83 0.03810965094534069 1106 84-85 -0.03059976963681521 1106 86-87 -0.22044590082956006 1106 88-89 -0.4432275265971164 1106 90-91 -3.5507675397953236 1106 92-93 -8.194468419034639 1106 94-95 -6.005000745555423 1106 96-97 -3.2983517905849773 1106 98-99 -2.111936400868828 1106 100-101 -1.6125138903967962 1107 1 1.7886496408623493 1107 2 1.0261912957117616 1107 3 0.7517688288880038 1107 4 0.8234016200896406 1107 5 0.5963729890331564 1107 6 0.804654759488848 1107 7 0.7526592731098276 1107 8 0.6148731514109116 1107 9 0.620062276836407 1107 10-11 0.8149218635674558 1107 12-13 0.5673265876576465 1107 14-15 0.7116533142648009 1107 16-17 0.7821317707139883 1107 18-19 0.6568558891259357 1107 20-21 0.5421917310658344 1107 22-23 0.4774280233795807 1107 24-25 0.5676611163717453 1107 26-27 0.6495170480606092 1107 28-29 0.4545082757098058 1107 30-31 0.5269822189736075 1107 32-33 0.83056362296891 1107 34-35 0.8251959462568834 1107 36-37 0.4937211205439027 1107 38-39 0.4736366246359154 1107 40-41 0.45566364165293294 1107 42-43 0.2808764611699246 1107 44-45 0.20638115157129988 1107 46-47 0.3795987867767181 1107 48-49 0.3457135454652125 1107 50-51 0.3531624294504496 1107 52-53 0.3976442434919143 1107 54-55 0.3115346741770857 1107 56-57 0.3167324278854764 1107 58-59 0.10047436830121015 1107 60-61 0.07787569480223056 1107 62-63 0.0158495062941455 1107 64-65 0.1164262969233043 1107 66-67 0.043920828093067854 1107 68-69 0.13235503525947934 1107 70-71 0.08936309190836766 1107 72-73 0.0878891281451395 1107 74-75 -0.22241018135971657 1107 76-77 -0.13610139135921528 1107 78-79 -0.1608858542114433 1107 80-81 -0.25895867538813633 1107 82-83 -0.43754251888634954 1107 84-85 -0.22976052242087874 1107 86-87 -0.15449626635339797 1107 88-89 -0.30057746834485144 1107 90-91 -3.244071514925725 1107 92-93 -4.462745002041247 1107 94-95 -2.802585532066347 1107 96-97 -1.6807828005029872 1107 98-99 -1.1503346565342127 1107 100-101 -0.7801810670967235 1108 1 1.6654876675931582 1108 2 1.030602938060504 1108 3 -0.4025289768257636 1108 4 -0.4142125001914323 1108 5 0.37659453554733346 1108 6 0.48219169021112407 1108 7 0.48680534706960543 1108 8 0.5180571734369792 1108 9 0.673811321894533 1108 10-11 0.6102244004981756 1108 12-13 0.6857961089386748 1108 14-15 0.5476661577602329 1108 16-17 0.5934172926606252 1108 18-19 0.6170305583945819 1108 20-21 0.46093705090518 1108 22-23 0.4290871639697116 1108 24-25 0.47988352138812473 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2.204720691323047 2311 88-89 2.8039163544804673 2311 90-91 3.347127412611986 2311 92-93 3.9923302236942586 2311 94-95 3.5088512664763183 2311 96-97 2.737277082752268 2311 98-99 2.566530860085498 2311 100-101 2.4753422437325696 2312 1 1.1211309179078732 2312 2 0.5281769677958152 2312 3 -0.1508128610110866 2312 4 -0.7239782955328451 2312 5 -0.2721963875308546 2312 6 -0.3612141301428409 2312 7 0.04987296322573087 2312 8 0.16808473660599788 2312 9 0.23216399058524217 2312 10-11 0.1518283301010186 2312 12-13 0.17265732221177643 2312 14-15 0.20866972451657517 2312 16-17 -0.09877307421749038 2312 18-19 -0.37551257590799025 2312 20-21 0.23750105505200025 2312 22-23 0.38870640141612967 2312 24-25 0.4475810300067522 2312 26-27 0.4228021699743678 2312 28-29 0.3858177306888919 2312 30-31 0.3575385775509261 2312 32-33 0.4394260849132081 2312 34-35 0.42918547133976404 2312 36-37 0.4262049948629638 2312 38-39 0.4054430622606944 2312 40-41 0.4462494284687253 2312 42-43 0.677085571050398 2312 44-45 0.4342539619567809 2312 46-47 0.3747570171977941 2312 48-49 0.3574016151715149 2312 50-51 0.42373283588988286 2312 52-53 0.3829973145984873 2312 54-55 0.3889877425734589 2312 56-57 0.32871489290133127 2312 58-59 0.3613577349097561 2312 60-61 0.5189136330639066 2312 62-63 0.5540724517378521 2312 64-65 0.692259908804818 2312 66-67 0.6046698140271403 2312 68-69 0.4942045876250347 2312 70-71 0.6469313211550265 2312 72-73 0.5094953950274146 2312 74-75 0.45322118196045835 2312 76-77 0.8539377258388008 2312 78-79 0.8308781913921663 2312 80-81 1.042095253044554 2312 82-83 1.0654125008529292 2312 84-85 1.3321179387284268 2312 86-87 1.8236831352347807 2312 88-89 2.3023724697722834 2312 90-91 3.099310745018446 2312 92-93 3.809385349471496 2312 94-95 3.295247099626712 2312 96-97 2.4754880341478014 2312 98-99 2.380191664009997 2312 100-101 2.256882703873437 2313 1 -7.145152431303686 2313 2 -2.7632659872141296 2313 3 -1.6763136180268248 2313 4 -0.9735187494260416 2313 5 -0.8818768992181489 2313 6 -0.3662657771956148 2313 7 -0.28412376362005887 2313 8 -0.2787012078057245 2313 9 -0.21221610642345468 2313 10-11 -0.17320665469936358 2313 12-13 -0.3958140594308901 2313 14-15 -0.5790820375709629 2313 16-17 -0.7994365994994439 2313 18-19 -0.6938639814272918 2313 20-21 -0.39327884904616184 2313 22-23 -1.1245578781144019 2313 24-25 -0.3346391805345448 2313 26-27 -0.4848221446595531 2313 28-29 -0.7497340408860609 2313 30-31 -0.2979198419267135 2313 32-33 -0.39252333931999317 2313 34-35 -0.23967212668685534 2313 36-37 -0.07705827663095022 2313 38-39 -0.2550261886692766 2313 40-41 -0.3249807122215671 2313 42-43 -0.07265915849930238 2313 44-45 -0.22460994124007883 2313 46-47 -1.2201914345765488 2313 48-49 -0.7796460036878621 2313 50-51 -0.6845845533348012 2313 52-53 -1.0371647025816202 2313 54-55 -0.7966928007385121 2313 56-57 -0.6158717527876334 2313 58-59 -0.521793921235119 2313 60-61 -0.14712235186693334 2313 62-63 -0.39651566845173747 2313 64-65 -0.5170924968449313 2313 66-67 -0.33197883974983355 2313 68-69 -0.28075558504571063 2313 70-71 -0.35074960165308866 2313 72-73 -0.201623347733495 2313 74-75 -0.11347042208275582 2313 76-77 0.13451987687036038 2313 78-79 0.0014311686579731031 2313 80-81 0.3384214536952328 2313 82-83 0.7079888052720804 2313 84-85 0.7691035929837255 2313 86-87 1.2652379625442691 2313 88-89 1.7677311924515244 2313 90-91 2.411468358462095 2313 92-93 3.244833649052074 2313 94-95 2.817581336898767 2313 96-97 2.1298165631654236 2313 98-99 1.919328389353618 2313 100-101 1.6460434442071552 2314 1 0.38951576870899274 2314 2 -0.2016606550440585 2314 3 -1.3547787746285138 2314 4 -0.4838032832745469 2314 5 -0.536171383542623 2314 6 -3.7061111066255066 2314 7 -2.9712758030660567 2314 8 -2.830099861101676 2314 9 -3.4838061471285187 2314 10-11 -2.7656510705784 2314 12-13 -1.5870213489399774 2314 14-15 -1.659227877359406 2314 16-17 -2.592475833288386 2314 18-19 -3.5723065787873445 2314 20-21 -3.2431188477233235 2314 22-23 -4.508300470080712 2314 24-25 -3.5798909129307503 2314 26-27 -2.2498433318425946 2314 28-29 -2.2524881483019072 2314 30-31 -2.250528800504714 2314 32-33 -2.438747737238238 2314 34-35 -1.9272856856965888 2314 36-37 -2.4154180622827184 2314 38-39 -3.740043633695393 2314 40-41 -2.9828370862309583 2314 42-43 -2.1289040624926017 2314 44-45 -2.65659121943175 2314 46-47 -2.1605693373072405 2314 48-49 -2.7152508354600933 2314 50-51 -3.6203998199177114 2314 52-53 -2.4862866736687828 2314 54-55 -3.0105556909435087 2314 56-57 -2.0609636216574145 2314 58-59 -1.474037177970704 2314 60-61 -1.8019026177667712 2314 62-63 -1.5486012270331244 2314 64-65 -2.1216236760397216 2314 66-67 -1.4908631258010168 2314 68-69 -1.2062800677284429 2314 70-71 -1.2201920261770702 2314 72-73 -2.3416651059527567 2314 74-75 -2.361976462880733 2314 76-77 -1.3839917427138957 2314 78-79 -2.3102555126098636 2314 80-81 -1.2342569030943302 2314 82-83 -0.8815951046736288 2314 84-85 -1.7823327574742898 2314 86-87 -0.5350422759931348 2314 88-89 0.4517023985126798 2314 90-91 1.6551762296689034 2314 92-93 2.8091399878052687 2314 94-95 2.4159156636786374 2314 96-97 1.7212924671000636 2314 98-99 1.7738878168134509 2314 100-101 1.6322351115398384 2315 1 -3.0124084531196154 2315 2 -2.0906089316202383 2315 3 -2.878964714079334 2315 4 -4.3399372859311995 2315 5 -4.0498661245963845 2315 6 -3.1861240161899964 2315 7 -6.168417754299941 2315 8 -3.347501393013708 2315 9 -2.7363980601795426 2315 10-11 -3.853013165938034 2315 12-13 -2.987206101132138 2315 14-15 -1.963359667121459 2315 16-17 -2.5098536078751152 2315 18-19 -1.975491680655736 2315 20-21 -1.445973240737331 2315 22-23 -0.8935213985222532 2315 24-25 -2.3585091480045257 2315 26-27 -3.9478473215744856 2315 28-29 -3.180555453432344 2315 30-31 -3.1156154463865917 2315 32-33 -4.0381654093464405 2315 34-35 -3.8062083406151643 2315 36-37 -2.9183920318087324 2315 38-39 -1.8068664782776267 2315 40-41 -1.5066570960356636 2315 42-43 -2.733289608033605 2315 44-45 -6.422839493202822 2315 46-47 -3.2376314270667876 2315 48-49 -2.313148534959094 2315 50-51 -3.290639081297808 2315 52-53 -3.372925294674534 2315 54-55 -1.7443008854269912 2315 56-57 -1.345628025133358 2315 58-59 -2.589604616014565 2315 60-61 -3.089373342635575 2315 62-63 -4.977541963240839 2315 64-65 -4.523052447254347 2315 66-67 -3.7928290090261676 2315 68-69 -2.2475978134478964 2315 70-71 -3.567261130727431 2315 72-73 -2.826451658131937 2315 74-75 -2.0211705933428803 2315 76-77 -3.603747710627939 2315 78-79 -2.059301599289263 2315 80-81 -1.6245209972592143 2315 82-83 -1.5303011253935423 2315 84-85 -2.796424264088717 2315 86-87 -2.1961843349755057 2315 88-89 -1.7023557546803438 2315 90-91 -0.7409865369718815 2315 92-93 -0.18342658207342133 2315 94-95 -0.09664518661892352 2315 96-97 -0.11613952806259853 2315 98-99 -0.27611061860566366 2315 100-101 -0.6333628738065702 2316 1 -7.073130423500398 2316 2 -5.914826644586885 2316 3 -3.3961988169207125 2316 4 -4.081675820720452 2316 5 -1.9648344430426583 2316 6 -3.061151129375652 2316 7 -3.5429048117294286 2316 8 -2.5515897933015808 2316 9 -7.13002284733512 2316 10-11 -6.987117321575866 2316 12-13 -6.316250986268436 2316 14-15 -5.085047219133294 2316 16-17 -4.9582633268810525 2316 18-19 -4.129962767196609 2316 20-21 -2.889710449701006 2316 22-23 -3.4407695744606706 2316 24-25 -3.2905382446715095 2316 26-27 -4.309069867338337 2316 28-29 -4.403564099686626 2316 30-31 -6.938679344135053 2316 32-33 -4.901228970644322 2316 34-35 -5.656474741135398 2316 36-37 -4.461465907419797 2316 38-39 -6.642222860929181 2316 40-41 -4.130194127416516 2316 42-43 -4.664008965665328 2316 44-45 -3.2463819393352438 2316 46-47 -4.249185560651341 2316 48-49 -5.397164924308118 2316 50-51 -4.849762385705155 2316 52-53 -6.536445535068477 2316 54-55 -5.396277190894683 2316 56-57 -4.605078159739875 2316 58-59 -3.3529586813627326 2316 60-61 -3.3721892868184256 2316 62-63 -4.365770503607813 2316 64-65 -3.075044471272715 2316 66-67 -3.9946404799933752 2316 68-69 -3.067658101186744 2316 70-71 -2.5904558236400455 2316 72-73 -2.111298922709839 2316 74-75 -2.188302420464055 2316 76-77 -2.9133918037385342 2316 78-79 -4.8033848266494275 2316 80-81 -5.790447437596274 2316 82-83 -3.3479683032197656 2316 84-85 -2.076410028023428 2316 86-87 -2.2426368578554587 2316 88-89 -1.252780129263396 2316 90-91 -0.8829832450132109 2316 92-93 -1.0787578045298165 2316 94-95 -1.6213630018350056 2316 96-97 -2.945524753991794 2316 98-99 -4.231587955241819 2316 100-101 -3.7688533635577066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29094.0 3 11018.0 4 10770.0 5 12074.0 6 13420.0 7 15825.0 8 18314.0 9 21566.0 10 24807.0 11 28240.0 12 31482.0 13 34309.0 14 36434.0 15 39645.0 16 43611.0 17 46267.0 18 45523.0 19 46511.0 20 49003.0 21 53270.0 22 58073.0 23 64710.0 24 71527.0 25 79584.0 26 88637.0 27 100101.0 28 111193.0 29 124790.0 30 139704.0 31 155670.0 32 170763.0 33 187673.0 34 209807.0 35 232824.0 36 228641.0 37 150829.0 38 34635.0 39 421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.668169865890675 18.049679769929934 12.496729538805509 23.78542082537388 2 16.78594373946224 18.765853225663324 38.58557103558435 25.862631999290087 3 20.590372449995616 22.327121159574215 28.058399000675514 29.02410738975465 4 13.706124196485215 14.756413474233387 34.14837106647406 37.389091262807334 5 14.978636278605503 36.198879793437285 32.957706682512416 15.864777245444792 6 35.60420912118264 33.33523860917231 16.09040948046323 14.97014278918182 7 31.18742420233781 31.047219613016868 18.9525140408788 18.812842143766524 8 28.262134726094057 33.68937075436827 18.409978847961334 19.63851567157634 9 27.482727945837393 15.82164551050883 18.48243852462655 38.21318801902723 10-11 26.76066740941635 24.96251481430518 25.77674009827377 22.500077678004697 12-13 27.493145988883395 22.113739214352705 26.390503677279263 24.002611119484634 14-15 23.928347348861852 23.691527940571273 24.24529015392995 28.13483455663693 16-17 24.033546998001356 26.8575351502819 25.02085103466935 24.088066817047395 18-19 24.299639750046296 25.256559793164783 26.989928662021832 23.453871794767082 20-21 24.577622361976488 24.61684723306623 27.212613894624845 23.592916510332444 22-23 24.270903219744103 24.41744819546293 27.370569257066705 23.941079327726257 24-25 24.21970580198399 24.70776166954549 27.31210026767564 23.760432260794882 26-27 23.700740585951007 25.028564036571915 27.245310966848656 24.025384410628416 28-29 23.697496102958304 25.58845640546078 27.05304756389807 23.660999927682845 30-31 23.77100558158971 25.14298648450814 26.939947097843405 24.146060836058748 32-33 23.60882263165918 25.10357232580146 26.99082906542033 24.296775977119033 34-35 24.18088470421034 25.254876532243735 26.88289216557115 23.681346597974773 36-37 24.341057147674444 24.804606821363258 26.892223768118207 23.96211226284409 38-39 23.92271586629863 25.023084977231232 26.95692924148041 24.097269914989727 40-41 24.03472415297032 25.052358927576883 27.108165221307406 23.804751698145385 42-43 23.899238118212917 25.08084857384093 26.751964835733283 24.267948472212872 44-45 23.846747944662376 25.165425281345545 27.065622306710157 23.922204467281922 46-47 24.344456041393705 25.454535764251297 26.53652816948324 23.664480024871757 48-49 24.109888344388235 25.63305458233024 26.19222125107033 24.064835822211194 50-51 23.89334967450651 25.848283368170293 26.458535902210308 23.799831055112893 52-53 24.32157004786701 25.391553133660267 26.353994068564297 23.932882749908426 54-55 23.691603800142747 25.694932195148308 26.473198671485566 24.140265333223383 56-57 23.656114009632994 25.879382163536334 26.346586697312585 24.117917129518087 58-59 23.79387617019704 25.428136469210276 26.27552543375114 24.50246192684154 60-61 23.310536446243468 25.665841758269863 26.768739311183726 24.25488248430294 62-63 23.544333610000074 25.60858837974828 26.75605968541695 24.0910183248347 64-65 23.286500653725025 25.769412418342096 26.531165037283017 24.41292189064986 66-67 23.337121618520275 25.83355300413739 26.35413728347623 24.475188093866105 68-69 23.666435566478786 25.360039349203394 26.658605693164674 24.314919391153143 70-71 23.56140540363857 25.486939276393066 26.23899408162091 24.712661238347458 72-73 23.550785543874564 25.67514978944716 26.217728943561035 24.556335723117243 74-75 23.49757606634511 26.0348120267483 26.050716287010943 24.416895619895644 76-77 23.731556873733474 25.602857273866984 26.139449211267436 24.526136641132105 78-79 23.784379958914926 25.8175206389495 26.03053460890539 24.367564793230187 80-81 23.928043651446796 25.781129188616752 25.858252953863314 24.432574206073138 82-83 23.793365342350317 25.847781856173423 25.92781921049362 24.431033590982636 84-85 23.62088729993921 26.017645207612578 25.83559204240195 24.525875450046264 86-87 23.711721266204112 25.8231336085168 26.025149646116525 24.439995479162565 88-89 23.64523886997545 25.85124235418641 25.59480471198392 24.908714063854216 90-91 23.46114888894705 25.803932586370177 26.038700575382762 24.696217949300014 92-93 23.74605340322482 25.393465022369472 26.392892122818395 24.467589451587312 94-95 23.63752591974404 25.220226667250074 26.325886731830213 24.816360681175674 96-97 24.100653751627714 24.839681968276825 25.90778146651912 25.15188281357635 98-99 23.866918468490095 25.363983447942285 25.4159975957905 25.35310048777712 100-101 24.23166681873877 25.11338264738143 25.594250642927058 25.06069989095274 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3708.0 1 2179.0 2 636.0 3 603.5 4 577.0 5 606.0 6 735.5 7 1190.0 8 1854.5 9 2176.5 10 1977.0 11 1796.0 12 1886.5 13 2010.0 14 2144.5 15 2274.0 16 2496.0 17 2764.0 18 3029.5 19 3325.5 20 3803.5 21 4475.5 22 5386.5 23 6550.5 24 7784.5 25 9350.5 26 11428.0 27 13851.5 28 16169.0 29 18392.0 30 21057.0 31 25091.5 32 31014.0 33 33231.0 34 32353.0 35 33970.5 36 37180.0 37 41507.5 38 46830.0 39 51244.5 40 55369.5 41 60808.5 42 65709.5 43 71234.5 44 77700.5 45 85174.0 46 92799.5 47 99914.0 48 106873.5 49 110586.5 50 111543.5 51 113861.0 52 118769.0 53 124199.0 54 126339.5 55 122644.0 56 116771.5 57 109692.0 58 102118.0 59 91997.5 60 78081.5 61 65583.5 62 54260.0 63 42165.0 64 31587.5 65 24076.0 66 18221.5 67 13873.0 68 10626.5 69 8075.5 70 6099.5 71 4438.0 72 3242.0 73 2491.0 74 1962.5 75 1498.5 76 1074.0 77 759.0 78 530.5 79 366.5 80 264.0 81 182.5 82 117.0 83 86.0 84 62.0 85 40.5 86 33.0 87 20.0 88 9.0 89 11.0 90 9.0 91 6.0 92 4.5 93 3.0 94 4.5 95 5.0 96 2.0 97 1.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13769314352666742 2 0.12461158586411843 3 0.12982293810367046 4 0.13904029580627952 5 0.12783766106003158 6 0.14269178751154388 7 0.1730027138028159 8 0.1591057744973438 9 0.17016660374047465 10-11 0.16493752581303298 12-13 0.15416030757613625 14-15 0.15896396899422674 16-17 0.15449709564603928 18-19 0.1702906835557021 20-21 0.17546658441947485 22-23 0.2102089326831551 24-25 0.2317810948448382 26-27 0.24503990938628353 28-29 0.24005899108929668 30-31 0.23089481045035654 32-33 0.23213560860263086 34-35 0.2221028692570987 36-37 0.23055802238045353 38-39 0.21852228030339285 40-41 0.22325503896992482 42-43 0.21588115280783757 44-45 0.2091276657218875 46-47 0.2243185802433028 48-49 0.2617020560025383 50-51 0.2631023853458193 52-53 0.2640950238676387 54-55 0.2622338266392273 56-57 0.23706334983594873 58-59 0.23784328010309258 60-61 0.20941127672812165 62-63 0.20226782450859962 64-65 0.2036149767882117 66-67 0.21082933175929228 68-69 0.21159153633854644 70-71 0.2177778014120283 72-73 0.24752150569083212 74-75 0.2500562790590496 76-77 0.2642545550586455 78-79 0.2600181156530232 80-81 0.2748899677924251 82-83 0.27309967331557217 84-85 0.24909909191300944 86-87 0.2526619551788256 88-89 0.2557994019352906 90-91 0.4417418678975384 92-93 0.588794174629932 94-95 0.3433111230464076 96-97 0.2890173410404624 98-99 0.320125923286768 100-101 0.34028003041728044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2820765.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.154903912890425 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1405678495769 42.316949435925316 2 11.428711238018598 12.378415178861363 3 4.0139215014816365 6.521205996798689 4 1.968868475827127 4.264955325021483 5 1.1321157092695626 3.065480872688348 6 0.680649363410703 2.2116300524325996 7 0.4886399265136016 1.8523573787842154 8 0.34240018796286115 1.4834119423107492 9 0.25415772891877336 1.2387498649483164 >10 1.4108323789164505 13.75114827044269 >50 0.09069720181671456 3.379257270844186 >100 0.0436617206687226 4.265377659251102 >500 0.0026238308044423137 1.0025229187361264 >1k 0.0020183313880066823 1.8303803747268397 >5k 1.3455542586711215E-4 0.4381574582281335 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6898 0.2445435901253738 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5128 0.18179465499607375 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3369 0.11943568500034564 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2951 0.10461700992461265 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2841 0.10071735858889344 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7725687889632777E-4 2 7.090275155853111E-5 0.0 0.0 0.0 1.7725687889632777E-4 3 7.090275155853111E-5 0.0 0.0 0.0 1.7725687889632777E-4 4 7.090275155853111E-5 0.0 0.0 0.0 1.7725687889632777E-4 5 1.4180550311706223E-4 0.0 0.0 0.0 1.7725687889632777E-4 6 1.4180550311706223E-4 0.0 0.0 0.0 1.7725687889632777E-4 7 2.4815963045485886E-4 0.0 0.0 0.0 2.1270825467559331E-4 8 2.4815963045485886E-4 0.0 0.0 0.0 2.1270825467559331E-4 9 2.4815963045485886E-4 0.0 0.0 0.0 2.1270825467559331E-4 10-11 2.4815963045485886E-4 0.0 0.0 3.545137577926556E-5 2.3043394256522609E-4 12-13 3.5451375779265554E-4 0.0 0.0 7.090275155853111E-5 5.140449487993506E-4 14-15 4.608678851304522E-4 3.545137577926556E-5 0.0 1.0635412733779667E-4 6.203990761371471E-4 16-17 5.317706366889833E-4 3.545137577926556E-5 0.0 1.59531191006695E-4 6.735761398060455E-4 18-19 7.444788913645766E-4 3.545137577926556E-5 0.0 2.1270825467559331E-4 7.267532034749438E-4 20-21 8.862843944816389E-4 3.545137577926556E-5 0.0 3.5451375779265554E-4 9.571871460401699E-4 22-23 9.217357702609045E-4 3.545137577926556E-5 0.0 6.913018276956783E-4 0.0010280898975987011 24-25 9.926385218194354E-4 3.545137577926556E-5 0.0 0.0010458155854883339 0.0010635412733779666 26-27 9.926385218194354E-4 3.545137577926556E-5 0.0 0.0021802596104248317 0.0011521697128261306 28-29 0.0010635412733779666 3.545137577926556E-5 0.0 0.006292619200819635 0.0012053467764950288 30-31 0.0011344440249364978 3.545137577926556E-5 0.0 0.022866137377626283 0.0012053467764950288 32-33 0.0011521697128261306 3.545137577926556E-5 0.0 0.05911516911192531 0.0013648779675017237 34-35 0.0012053467764950288 3.545137577926556E-5 0.0 0.11153002820156943 0.0014889577827291534 36-37 0.00127624952805356 3.545137577926556E-5 0.0 0.20405811898545254 0.0015066834706187861 38-39 0.001347152279612091 3.545137577926556E-5 0.0 0.34793752758560176 0.001577586222177317 40-41 0.0014003293432809892 3.545137577926556E-5 0.0 0.4995807874814102 0.0015953119100669498 42-43 0.0015066834706187861 3.545137577926556E-5 0.0 0.6488665308878975 0.0016130375979565826 44-45 0.0015953119100669498 3.545137577926556E-5 0.0 0.8320969666030313 0.0017016660374047465 46-47 0.0017902944768529105 3.545137577926556E-5 0.0 1.0537921450386687 0.0017725687889632777 48-49 0.001843471540521809 3.545137577926556E-5 0.0 1.2899514847922462 0.0018966486041907071 50-51 0.001843471540521809 3.545137577926556E-5 0.0 1.5463181087400049 0.001985277043638871 52-53 0.001843471540521809 3.545137577926556E-5 0.0 1.7887523420065126 0.0021270825467559333 54-55 0.001843471540521809 3.545137577926556E-5 0.0 2.04324004303797 0.0022157109862040968 56-57 0.001843471540521809 3.545137577926556E-5 0.0 2.337238302375419 0.0022866137377626286 58-59 0.001843471540521809 3.545137577926556E-5 0.0 2.6528087238745517 0.002304339425652261 60-61 0.001843471540521809 7.090275155853111E-5 0.0 2.9889232176377685 0.0023575164893211596 62-63 0.001843471540521809 7.090275155853111E-5 0.0 3.31273608400558 0.002446144928769323 64-65 0.001843471540521809 7.090275155853111E-5 0.0 3.6280406201863675 0.0025170476803278545 66-67 0.0019498256678596054 7.090275155853111E-5 0.0 3.9989329135890443 0.0026056761197760184 68-69 0.0019498256678596054 7.090275155853111E-5 0.0 4.408183595584886 0.002623401807665651 70-71 0.002003002731528504 7.090275155853111E-5 0.0 4.839715467257996 0.0026765788713345494 72-73 0.0020916311709766678 7.090275155853111E-5 0.0 5.276511868234326 0.002782932998672346 74-75 0.0021625339225351987 7.090275155853111E-5 0.0 5.6871451538855595 0.0028892871260101424 76-77 0.0021625339225351987 7.090275155853111E-5 0.0 6.129383341044008 0.0029424641896790412 78-79 0.0021979852983144642 7.090275155853111E-5 0.0 6.622015658872682 0.0030133669412375722 80-81 0.002446144928769323 7.090275155853111E-5 0.0 7.115800146414182 0.003101995380685736 82-83 0.0025170476803278545 1.0635412733779666E-4 0.0 7.589731863519294 0.003261526571692431 84-85 0.0025347733682174875 1.0635412733779666E-4 0.0 8.037181402917295 0.003261526571692431 86-87 0.00255249905610712 1.0635412733779666E-4 0.0 8.488973735848255 0.003367880699030228 88-89 0.00255249905610712 1.0635412733779666E-4 0.0 8.97373584825393 0.003491960514257657 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5535 0.0 37.970303 1 GTATCAA 9260 0.0 36.27273 1 TATCAAC 11045 0.0 31.054377 2 ATCAACG 11425 0.0 29.896927 3 TCAACGC 11445 0.0 29.846802 4 CAACGCA 11575 0.0 29.511591 5 AACGCAG 12060 0.0 28.364103 6 TACATGG 10170 0.0 26.402542 2 GTACATG 10135 0.0 26.026094 1 ACATGGG 10620 0.0 24.479713 3 ACGCAGA 13900 0.0 24.336372 7 CGCAGAG 14045 0.0 23.982508 8 TAAGGTG 1275 0.0 21.954468 5 GCAGAGT 15000 0.0 21.12726 9 GAGTACT 7785 0.0 20.626945 12-13 AGAGTAC 11815 0.0 20.437445 10-11 GTAAGGT 1330 0.0 20.333134 4 GTACTTT 7915 0.0 20.17151 14-15 CATGGGG 7375 0.0 19.68523 4 GTATAGG 560 0.0 18.637714 1 >>END_MODULE