FastQCFastQC Report
Fri 27 May 2016
SRR939881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820765
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81620.28935412911036545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.23989945989829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43300.15350445712421984No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36370.12893665370918883No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34410.12198818405645276No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32650.11574874191930204No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28590.10135548335292022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55800.043.0328061
GTATCAA92450.035.5665661
TCAACGC114600.028.4767994
ATCAACG114650.028.4257683
TATCAAC116250.028.1567442
CAACGCA116700.028.0451875
AACGCAG121100.027.0502766
TAAGGTG15250.024.7394395
GTACATG106550.024.3957541
TACATGG104850.024.290772
AGGTAAG12700.023.7679752
ACATGGG103800.023.4455593
GGTAAGG14500.023.4192373
GTAAGGT15650.023.2031064
ACGCAGA141050.023.1574737
CGCAGAG141350.023.0749788
GAGTACA77250.020.8243731
GCAGAGT156450.020.6068469
GAGTACT87550.020.53889712-13
GTACTTT89200.020.1593314-15
Produced by FastQC (version 0.11.5)