##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939881_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2820765 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.385351491528006 31.0 31.0 34.0 30.0 34.0 2 31.597379434302397 31.0 31.0 34.0 30.0 34.0 3 31.65652580062501 31.0 31.0 34.0 30.0 34.0 4 35.47066061866196 37.0 35.0 37.0 33.0 37.0 5 35.18843292511074 37.0 35.0 37.0 32.0 37.0 6 35.21488142401086 37.0 35.0 37.0 32.0 37.0 7 35.12487605312743 37.0 35.0 37.0 32.0 37.0 8 35.05661903774331 37.0 35.0 37.0 32.0 37.0 9 36.67142459581 39.0 35.0 39.0 32.0 39.0 10-11 36.51873693838374 38.0 35.0 39.0 32.0 39.0 12-13 36.45490638177941 38.0 35.0 39.0 32.0 39.0 14-15 37.50312415249054 39.0 36.0 41.0 32.0 41.0 16-17 37.41713347265724 39.0 36.0 41.0 32.0 41.0 18-19 37.36616892935072 39.0 36.0 41.0 32.0 41.0 20-21 37.27210508496808 39.0 36.0 40.0 31.0 41.0 22-23 37.126906885188944 39.0 36.0 40.0 31.0 41.0 24-25 36.94769876256973 39.0 36.0 40.0 31.0 41.0 26-27 36.63188886702721 38.5 35.0 40.0 30.0 41.0 28-29 36.39710060923189 38.0 35.0 40.0 30.0 41.0 30-31 36.12940337107132 38.0 35.0 40.0 29.5 41.0 32-33 36.07291054022579 38.0 35.0 40.0 30.0 41.0 34-35 36.166794646133226 38.0 35.0 40.0 30.0 41.0 36-37 36.13776493256262 38.0 35.0 40.0 30.0 41.0 38-39 35.984736410158234 38.0 35.0 40.0 29.0 41.0 40-41 35.78917935382778 38.0 34.5 40.0 28.5 41.0 42-43 35.56418577938963 38.0 34.0 40.0 27.5 41.0 44-45 35.30198120013542 38.0 34.0 40.0 27.0 41.0 46-47 34.98772921530152 38.0 33.5 40.0 26.0 41.0 48-49 34.64373423521633 38.0 33.0 40.0 25.5 41.0 50-51 34.32799541968225 37.5 33.0 40.0 24.0 41.0 52-53 33.99615051236101 37.0 33.0 40.0 24.0 41.0 54-55 33.632626255643416 37.0 32.5 40.0 23.0 41.0 56-57 32.26606682938848 35.5 30.5 39.0 15.0 40.5 58-59 28.829017482845963 34.0 23.0 38.0 7.0 40.0 60-61 28.78283391207704 33.5 24.0 38.0 7.5 40.0 62-63 30.566083491535096 34.0 28.5 38.0 7.5 40.0 64-65 30.049429144221513 34.0 29.0 37.5 2.0 40.0 66-67 24.63611857067143 28.0 16.5 35.5 2.0 38.0 68-69 17.056962916088366 13.0 7.0 29.0 2.0 34.5 70-71 15.891509572757744 12.5 7.5 25.5 2.0 33.0 72-73 17.965212805745956 17.0 7.0 29.0 2.0 34.0 74-75 18.68306115539579 17.5 7.5 30.5 2.0 34.5 76-77 19.688745251731355 22.0 7.5 30.5 2.0 34.0 78-79 22.755838752962404 27.5 11.0 33.5 2.0 35.0 80-81 25.404856129454245 31.5 21.0 34.0 2.0 35.5 82-83 26.36227335492322 32.5 25.0 35.0 2.0 35.5 84-85 26.371685695192618 33.0 25.0 35.0 2.0 35.0 86-87 26.105681791996144 33.0 24.0 35.0 2.0 35.0 88-89 25.67129679360032 32.0 22.0 35.0 2.0 35.0 90-91 24.106016452983496 31.5 10.0 34.0 2.0 35.0 92-93 22.041483427368107 30.5 2.0 34.0 2.0 35.0 94-95 17.64328701611088 20.0 2.0 33.0 2.0 35.0 96-97 16.304820323564705 13.5 2.0 32.5 2.0 34.5 98-99 15.217397585406795 2.0 2.0 31.5 2.0 34.0 100-101 14.577483413187558 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4034635084602627 1101 2 0.4311632585631635 1101 3 0.43146641394243446 1101 4 0.37844462539608514 1101 5 0.36718815703011387 1101 6 0.5238798183123237 1101 7 0.5025345500275904 1101 8 0.450259868027409 1101 9 0.6266409295349149 1101 10-11 0.532840645106468 1101 12-13 0.6867036713165433 1101 14-15 0.6760298404280078 1101 16-17 0.7597236247163863 1101 18-19 0.746688396888139 1101 20-21 0.7330869468942325 1101 22-23 0.6278172274382783 1101 24-25 0.6199646949022366 1101 26-27 0.8888884846705594 1101 28-29 0.6177628233269772 1101 30-31 0.787043406077153 1101 32-33 0.7336094429414146 1101 34-35 0.9186459335049832 1101 36-37 0.8033495266439417 1101 38-39 0.8859382379668048 1101 40-41 0.8307793373649304 1101 42-43 0.8218365409079809 1101 44-45 0.5658233105255306 1101 46-47 0.2929890644044164 1101 48-49 0.43726042196541925 1101 50-51 0.34987164964880435 1101 52-53 0.4764500428299314 1101 54-55 0.39405876069408663 1101 56-57 1.1274492908094018 1101 58-59 -7.816123215843337 1101 60-61 -9.176463852083767 1101 62-63 -2.601542405879524 1101 64-65 0.06771843963368696 1101 66-67 -1.0725850462619455 1101 68-69 -3.0324079074491213 1101 70-71 -3.9446599951541614 1101 72-73 -6.421858818797952 1101 74-75 -7.507932808652011 1101 76-77 -8.678680997357667 1101 78-79 -11.53833567547095 1101 80-81 -7.801345441975858 1101 82-83 -3.915352711666749 1101 84-85 -3.0388920673896678 1101 86-87 -3.0044968200811866 1101 88-89 -2.9662034512632367 1101 90-91 -8.34487350967782 1101 92-93 -13.250252139483784 1101 94-95 -11.875567748088574 1101 96-97 -11.629439620416871 1101 98-99 -11.422570261527795 1101 100-101 -11.20167564187009 1102 1 0.499303570896938 1102 2 0.5351616976462701 1102 3 0.4937949540384885 1102 4 0.4506617150853387 1102 5 0.5630491413950836 1102 6 0.5302927419519889 1102 7 0.511501594464356 1102 8 0.6051338545480434 1102 9 0.663632779385992 1102 10-11 0.7235281537749714 1102 12-13 0.7612944595560691 1102 14-15 0.8404033227066705 1102 16-17 0.9053525872473287 1102 18-19 0.8275404835566391 1102 20-21 0.8871616687383721 1102 22-23 0.9277700943301497 1102 24-25 0.8903124455598643 1102 26-27 0.965493302355938 1102 28-29 0.962344631592785 1102 30-31 0.8900668597938477 1102 32-33 0.7694917253592379 1102 34-35 0.6767649346122013 1102 36-37 0.7925024471335647 1102 38-39 0.7184652542882617 1102 40-41 0.7938862202091883 1102 42-43 0.9362489285701514 1102 44-45 0.7833185090605284 1102 46-47 0.7474092331432942 1102 48-49 0.8232412020007303 1102 50-51 0.7609111403761588 1102 52-53 0.753250092666427 1102 54-55 0.5231458828946032 1102 56-57 1.1690286848257259 1102 58-59 -8.495683314670067 1102 60-61 -9.743446933531189 1102 62-63 -2.912444611144796 1102 64-65 -0.026035894614100386 1102 66-67 -1.1416926437428536 1102 68-69 -3.6966391676309307 1102 70-71 -4.2210122216939805 1102 72-73 -6.570681313867194 1102 74-75 -7.470075872327548 1102 76-77 -8.408454899485356 1102 78-79 -11.63843123461536 1102 80-81 -7.972331664063304 1102 82-83 -3.7712823152549753 1102 84-85 -2.7103625498399317 1102 86-87 -2.507164953633609 1102 88-89 -2.6273287454043945 1102 90-91 -8.241476282185936 1102 92-93 -13.965800893453663 1102 94-95 -12.089378689081684 1102 96-97 -11.804947564872677 1102 98-99 -11.39597590480657 1102 100-101 -11.155153148446391 1103 1 0.26427384446808944 1103 2 0.2978198865381536 1103 3 0.31929862967036016 1103 4 0.15559243373966325 1103 5 0.3029095598411544 1103 6 0.28212427184617184 1103 7 0.32427800022624353 1103 8 0.31444633708136394 1103 9 0.34331914781665773 1103 10-11 0.3164292353146152 1103 12-13 0.37414455605991037 1103 14-15 0.43998744867055706 1103 16-17 0.41468951986331604 1103 18-19 0.537395202658324 1103 20-21 0.5186498131329387 1103 22-23 0.5103150532783687 1103 24-25 0.35136792956804186 1103 26-27 0.2679717314112793 1103 28-29 0.4981788665802114 1103 30-31 0.49747520865239636 1103 32-33 0.3614596571179689 1103 34-35 0.44864880627951464 1103 36-37 0.2920875029077763 1103 38-39 0.22716618354855456 1103 40-41 0.3467348054438446 1103 42-43 0.40384713421957485 1103 44-45 0.3185169371310579 1103 46-47 0.13741444008109482 1103 48-49 0.09501329999068986 1103 50-51 0.24783813821769485 1103 52-53 0.2558424955136971 1103 54-55 0.24306331049969998 1103 56-57 0.8356740051427636 1103 58-59 -6.7649007915420185 1103 60-61 -9.051845242271693 1103 62-63 -3.1296003711363944 1103 64-65 -0.8030973037403335 1103 66-67 -2.140333221765335 1103 68-69 -4.242924077752322 1103 70-71 -4.769873557640212 1103 72-73 -7.4648760365781115 1103 74-75 -8.184807970401321 1103 76-77 -9.024891444645457 1103 78-79 -12.06549069244951 1103 80-81 -8.658312062614058 1103 82-83 -4.951405498097856 1103 84-85 -3.997216367680398 1103 86-87 -3.716044092173515 1103 88-89 -3.9106654574850914 1103 90-91 -9.16379580272859 1103 92-93 -14.405774434456857 1103 94-95 -12.289114321744272 1103 96-97 -12.106907919332087 1103 98-99 -11.7214507694144 1103 100-101 -11.449535745225896 1104 1 0.11939019013771812 1104 2 0.0977243806196455 1104 3 0.15279932509732674 1104 4 0.14887923464648622 1104 5 0.02712342300252857 1104 6 0.09929730588151386 1104 7 0.0842143511346336 1104 8 0.15432450823756483 1104 9 0.18073254225655688 1104 10-11 0.1947314181620996 1104 12-13 0.040720816452115116 1104 14-15 0.18983273000489476 1104 16-17 0.29814186282501964 1104 18-19 0.3097307789127157 1104 20-21 0.1489678925739426 1104 22-23 0.2308961982610569 1104 24-25 0.33172822806669444 1104 26-27 0.23383758871409555 1104 28-29 0.16205930986417627 1104 30-31 0.17937910830062265 1104 32-33 0.2255792571829076 1104 34-35 0.26059287974759826 1104 36-37 0.14879703579114079 1104 38-39 0.15391600323559373 1104 40-41 0.24928423932571775 1104 42-43 0.19816757571424404 1104 44-45 0.16358400571269982 1104 46-47 0.13040638779310143 1104 48-49 0.10662140798662278 1104 50-51 0.028643696745156433 1104 52-53 0.051462942726729466 1104 54-55 -0.10470695372566041 1104 56-57 0.4671387551118684 1104 58-59 -6.622334516152936 1104 60-61 -8.930887930327504 1104 62-63 -2.809538582819197 1104 64-65 -0.6267768225860344 1104 66-67 -2.0634495738911802 1104 68-69 -4.684277806126296 1104 70-71 -5.046264273229028 1104 72-73 -7.925907627316576 1104 74-75 -8.83425328899726 1104 76-77 -9.372408129358792 1104 78-79 -12.34409254759779 1104 80-81 -8.81989783901123 1104 82-83 -5.399688132437927 1104 84-85 -4.567928562370433 1104 86-87 -4.429780031451859 1104 88-89 -4.600139561592538 1104 90-91 -10.0262431269027 1104 92-93 -14.900046327493918 1104 94-95 -12.540525178429132 1104 96-97 -12.177553303689223 1104 98-99 -11.5679520984731 1104 100-101 -11.128524431625227 1105 1 0.12849278272463494 1105 2 0.120737037101879 1105 3 0.07468557764471129 1105 4 0.12232992646267604 1105 5 0.17303879618488338 1105 6 0.12288264520088177 1105 7 0.16307986451894152 1105 8 0.15816665462985213 1105 9 0.21764951665246457 1105 10-11 0.2573966151343612 1105 12-13 0.23899501086548725 1105 14-15 0.25451333003860555 1105 16-17 0.32763215594670214 1105 18-19 0.28146581637651735 1105 20-21 0.3163550414944609 1105 22-23 0.27786816170129214 1105 24-25 0.21973042686234123 1105 26-27 0.3293061160573032 1105 28-29 0.18713525258822727 1105 30-31 0.15001379027969364 1105 32-33 0.22032790879254804 1105 34-35 0.3208892957873104 1105 36-37 0.2377563235958675 1105 38-39 0.19406317697875863 1105 40-41 0.3759223229259163 1105 42-43 0.40106297586441286 1105 44-45 0.38010360717141367 1105 46-47 0.3860340979811099 1105 48-49 0.30594520171354844 1105 50-51 0.29346306223555274 1105 52-53 0.47444667776802163 1105 54-55 0.19645766098712159 1105 56-57 0.8398192938551077 1105 58-59 -6.290456671892027 1105 60-61 -8.285526565541947 1105 62-63 -2.291308019559768 1105 64-65 -0.06759704231237151 1105 66-67 -1.522519031317291 1105 68-69 -4.1074631293569155 1105 70-71 -4.55886233798241 1105 72-73 -6.07776386668651 1105 74-75 -6.4756542359350355 1105 76-77 -8.391694516745213 1105 78-79 -11.552800008180355 1105 80-81 -7.9518341965185435 1105 82-83 -4.020495002333583 1105 84-85 -3.1574203111407932 1105 86-87 -2.991006817686017 1105 88-89 -3.087485952349933 1105 90-91 -8.265378912110243 1105 92-93 -11.527351902628373 1105 94-95 -11.458963363158611 1105 96-97 -11.773591162046944 1105 98-99 -11.314349367982624 1105 100-101 -10.915605645329775 1106 1 0.2336498832559073 1106 2 0.23314522055763476 1106 3 0.21643227753800076 1106 4 0.15228716429746925 1106 5 0.050436163358760666 1106 6 0.11407600879491753 1106 7 0.22067306550578536 1106 8 0.2269440258896509 1106 9 0.1889654993422667 1106 10-11 0.25116124381855087 1106 12-13 0.17441277290106427 1106 14-15 0.3813631423870092 1106 16-17 0.2482977617258939 1106 18-19 0.30121700300455956 1106 20-21 0.29325397044404156 1106 22-23 0.23748119703206072 1106 24-25 0.22549621547091903 1106 26-27 0.3466597410672847 1106 28-29 0.39621739047871785 1106 30-31 0.36386292181418156 1106 32-33 0.32553122222515896 1106 34-35 0.4398583501248652 1106 36-37 0.44896120865602285 1106 38-39 0.4443735098983126 1106 40-41 0.40880620500666964 1106 42-43 0.32237102523145467 1106 44-45 0.33946023543663983 1106 46-47 0.3280252401410664 1106 48-49 0.4824506793358694 1106 50-51 0.4945286552592094 1106 52-53 0.42478770694294354 1106 54-55 0.16867165173883336 1106 56-57 1.0319306673362334 1106 58-59 -6.474505935752266 1106 60-61 -8.801202072419049 1106 62-63 -2.21179240335837 1106 64-65 0.1551570495710628 1106 66-67 -0.9820078816284514 1106 68-69 -3.8305095356654117 1106 70-71 -4.423050917212452 1106 72-73 -5.744958514130683 1106 74-75 -6.063198711801661 1106 76-77 -7.905048554495384 1106 78-79 -11.420168138726552 1106 80-81 -7.557853657011119 1106 82-83 -3.464504539950493 1106 84-85 -2.4517688816389978 1106 86-87 -2.1984742910627197 1106 88-89 -2.4109795673214087 1106 90-91 -7.708066812600453 1106 92-93 -11.171882395106234 1106 94-95 -11.480231992207846 1106 96-97 -11.699924880639024 1106 98-99 -11.282752187889976 1106 100-101 -11.036940177994843 1107 1 -0.01178863061772617 1107 2 0.08287979987406402 1107 3 0.05485225394505022 1107 4 -0.030838244433176953 1107 5 0.016412824904236345 1107 6 0.022337035265536542 1107 7 0.05286273529836194 1107 8 0.10192733768477069 1107 9 -0.07638634538233191 1107 10-11 0.07704342404908004 1107 12-13 0.09404505091404047 1107 14-15 0.09571720194929156 1107 16-17 -0.020322168058484635 1107 18-19 -0.04433587722314769 1107 20-21 -0.008383507509279298 1107 22-23 0.08861139266997498 1107 24-25 0.1430993772599365 1107 26-27 0.18214161559075848 1107 28-29 0.20091845779371198 1107 30-31 0.2585840775558097 1107 32-33 0.11264168301779165 1107 34-35 -0.037758921019865 1107 36-37 -0.07757029919630298 1107 38-39 0.08198451253609562 1107 40-41 -0.0562707276178358 1107 42-43 -0.12369745533878529 1107 44-45 -0.11029764217831683 1107 46-47 -0.1512616337513819 1107 48-49 -0.1340583489829683 1107 50-51 -0.01975481841149218 1107 52-53 0.011148906449399476 1107 54-55 -0.14364797223418435 1107 56-57 0.5187403362735452 1107 58-59 -6.975111469873905 1107 60-61 -8.956710392532194 1107 62-63 -3.123878744479878 1107 64-65 -0.8358444226415109 1107 66-67 -2.2426209280498206 1107 68-69 -4.5216467168815875 1107 70-71 -5.024429871404374 1107 72-73 -6.5011818455970225 1107 74-75 -6.973675032281946 1107 76-77 -9.17978399429738 1107 78-79 -12.267921124089954 1107 80-81 -8.596683002906499 1107 82-83 -4.72176169267156 1107 84-85 -3.7736885910347695 1107 86-87 -3.5512860840956044 1107 88-89 -3.5683689610285008 1107 90-91 -8.918959438604222 1107 92-93 -12.160872025994433 1107 94-95 -11.994654056205025 1107 96-97 -12.281216386029836 1107 98-99 -11.530371576350706 1107 100-101 -11.040070976494157 1108 1 -0.04416494200819798 1108 2 -0.015592671326402296 1108 3 -0.07265139563477518 1108 4 -0.025269325994663916 1108 5 -0.2552095173317994 1108 6 -0.08977030398276042 1108 7 -0.008703596287709559 1108 8 -0.006884033876495721 1108 9 0.05390176341674646 1108 10-11 0.08090952039798793 1108 12-13 -0.08512000842472389 1108 14-15 -0.018751491085112093 1108 16-17 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3.390797348154546 2312 82-83 2.0272538277317196 2312 84-85 1.8100909840061128 2312 86-87 1.661058757041598 2312 88-89 1.79273799695439 2312 90-91 3.2407758201745622 2312 92-93 5.087115990411302 2312 94-95 8.927141472870886 2312 96-97 9.772837393341938 2312 98-99 10.271440304256016 2312 100-101 9.343549374386207 2313 1 -0.32729108182079614 2313 2 -0.30512120677271426 2313 3 -0.3296772749660626 2313 4 -0.3987521961214 2313 5 -0.4172511513141899 2313 6 -0.22729955434601123 2313 7 -0.31536738342781234 2313 8 -0.3576409852853075 2313 9 -0.4776422916848162 2313 10-11 -0.47666363447088855 2313 12-13 -0.4397405315056204 2313 14-15 -0.5732597705514451 2313 16-17 -0.454444268497852 2313 18-19 -0.3991834083946699 2313 20-21 -0.4336804137966439 2313 22-23 -0.4431836093469599 2313 24-25 -0.44395648981907954 2313 26-27 -0.5829951084826348 2313 28-29 -0.5982845832244195 2313 30-31 -0.6898866006769993 2313 32-33 -0.4720576948236257 2313 34-35 -0.5631974210798276 2313 36-37 -0.8022864756829264 2313 38-39 -0.8702585926603135 2313 40-41 -0.9898602047066305 2313 42-43 -0.5487856277842766 2313 44-45 -0.591907940865319 2313 46-47 -0.47717283987412173 2313 48-49 -0.4632275292381607 2313 50-51 -0.3337579746307213 2313 52-53 -0.4600671737334707 2313 54-55 -0.5049801075246094 2313 56-57 0.5070594178628127 2313 58-59 4.095432619074419 2313 60-61 3.8656870893972233 2313 62-63 1.2123215182658313 2313 64-65 1.200112536849133 2313 66-67 1.8071840114241766 2313 68-69 0.22446433769902185 2313 70-71 2.7216759591519004 2313 72-73 8.110074200643314 2313 74-75 9.982144031136798 2313 76-77 8.796590242086541 2313 78-79 6.413407486119699 2313 80-81 3.555852375094819 2313 82-83 2.0720374358703317 2313 84-85 1.6609727195684556 2313 86-87 1.7042838615018603 2313 88-89 1.4965672513630714 2313 90-91 2.8986939478354827 2313 92-93 4.810717685494058 2313 94-95 8.692624717721007 2313 96-97 9.485033055226694 2313 98-99 9.756810530411995 2313 100-101 8.9382718085377 2314 1 -0.4108040754321607 2314 2 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3.615616714799639 2314 62-63 1.1357605998487905 2314 64-65 0.9933534947468168 2314 66-67 1.4731135328887248 2314 68-69 -0.5219189985411603 2314 70-71 2.256989194025101 2314 72-73 7.9584583309845485 2314 74-75 9.535358004559413 2314 76-77 8.610132991849433 2314 78-79 6.270508716090497 2314 80-81 3.322697839171493 2314 82-83 1.8263363395226833 2314 84-85 1.3197527724744056 2314 86-87 1.278267790109144 2314 88-89 1.516103190439729 2314 90-91 2.6567068585721465 2314 92-93 4.793858983118959 2314 94-95 8.651046716485563 2314 96-97 9.338303790929626 2314 98-99 9.364448132927608 2314 100-101 8.630268868355905 2315 1 -0.7142016261187578 2315 2 -0.5187642208363314 2315 3 -0.5257710348722142 2315 4 -0.4658411094519934 2315 5 -0.699123756198226 2315 6 -0.7074905214482783 2315 7 -0.7089976480813505 2315 8 -0.7878473727520259 2315 9 -1.0319070450264718 2315 10-11 -0.9179258506104091 2315 12-13 -0.741809058868995 2315 14-15 -0.8953903147757813 2315 16-17 -1.0015202483556607 2315 18-19 -0.8879851867676294 2315 20-21 -0.8538830443390282 2315 22-23 -0.9292526189546209 2315 24-25 -0.9161804560922491 2315 26-27 -1.0098047721019086 2315 28-29 -1.0087939092726828 2315 30-31 -0.9969071306221551 2315 32-33 -0.9008475663846696 2315 34-35 -0.9469146308201033 2315 36-37 -0.9630243462573915 2315 38-39 -0.9928603067340305 2315 40-41 -0.9071732552841922 2315 42-43 -0.9512648603982328 2315 44-45 -0.893402806129366 2315 46-47 -0.9370665988804134 2315 48-49 -0.8707717073552317 2315 50-51 -0.7652781096591141 2315 52-53 -0.7640922365295353 2315 54-55 -0.9192114882525857 2315 56-57 0.38234522634188295 2315 58-59 3.9336776997940923 2315 60-61 3.6187272940095063 2315 62-63 1.0783086833688493 2315 64-65 1.103847690658064 2315 66-67 1.5149411091888787 2315 68-69 5.367300269520555 2315 70-71 4.537977237487842 2315 72-73 8.785910274806682 2315 74-75 9.95419630780841 2315 76-77 8.785837298821619 2315 78-79 6.231496524612936 2315 80-81 3.2551310844798493 2315 82-83 1.642829128425074 2315 84-85 1.069770671482278 2315 86-87 1.11456316029658 2315 88-89 1.1270167345354416 2315 90-91 2.523509885129819 2315 92-93 4.371999215946822 2315 94-95 8.402302305305199 2315 96-97 9.13506675915324 2315 98-99 9.572078143270046 2315 100-101 8.94786106647458 2316 1 -0.3940677398148722 2316 2 -0.45335402186260865 2316 3 -0.477891593527378 2316 4 -0.30767232586279647 2316 5 -0.5422457862989916 2316 6 -0.4770447927672876 2316 7 -0.5339200147378378 2316 8 -0.6726424874895613 2316 9 -0.7370433311692821 2316 10-11 -0.6322708417812137 2316 12-13 -0.5533133557109338 2316 14-15 -0.6603126604041094 2316 16-17 -0.9360142661065822 2316 18-19 -0.9001554980527615 2316 20-21 -0.6361886350437373 2316 22-23 -0.7647392184958903 2316 24-25 -2.1535020781415355 2316 26-27 -1.446374689950133 2316 28-29 -1.0688679494291122 2316 30-31 -0.8124071461304254 2316 32-33 -0.7092399581855844 2316 34-35 -0.694244764017732 2316 36-37 -0.7858542005690268 2316 38-39 -1.0366357869515355 2316 40-41 -0.8059861630005258 2316 42-43 -0.7740415134614338 2316 44-45 -0.6142605868908149 2316 46-47 -0.7735859440003807 2316 48-49 -0.9087264347750548 2316 50-51 -0.8056981787261179 2316 52-53 -0.8268178752730861 2316 54-55 -0.7830492538842648 2316 56-57 0.46750060605528176 2316 58-59 4.1328679578674645 2316 60-61 3.8767798970067595 2316 62-63 1.2994919274989734 2316 64-65 0.9227055355553979 2316 66-67 5.961634930221351 2316 68-69 8.62691037586523 2316 70-71 5.441983154812467 2316 72-73 9.30131098482255 2316 74-75 10.152378160399945 2316 76-77 8.780930324779309 2316 78-79 6.315010603755578 2316 80-81 3.3112408430712996 2316 82-83 1.8821363871886945 2316 84-85 1.5272109344184095 2316 86-87 1.3977335867108955 2316 88-89 1.4767533305282114 2316 90-91 2.8378622887275284 2316 92-93 4.691235935742895 2316 94-95 8.684486142955176 2316 96-97 9.208572800281171 2316 98-99 9.388933686635108 2316 100-101 8.531641467281855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 23.0 4 82.0 5 172.0 6 346.0 7 849.0 8 1985.0 9 3370.0 10 5279.0 11 8031.0 12 11067.0 13 14933.0 14 19420.0 15 26331.0 16 35562.0 17 45464.0 18 50174.0 19 52689.0 20 54679.0 21 53436.0 22 51534.0 23 49392.0 24 51128.0 25 55206.0 26 72882.0 27 105021.0 28 145565.0 29 188741.0 30 219512.0 31 234306.0 32 239665.0 33 244879.0 34 243449.0 35 227565.0 36 181180.0 37 100293.0 38 25680.0 39 872.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82885639740752 18.07008685328978 13.602181154389223 24.49887559491348 2 17.025387653720472 19.67781585841142 37.49090183268023 25.805894655187878 3 20.45079312632127 22.60825696562487 28.707120605614335 28.233829302439524 4 14.206536170152424 15.522633044581877 33.86343775536069 36.40739302990501 5 14.726799062370521 36.528883755512545 33.14147023934525 15.602846942771684 6 35.625619291220644 33.92561946847753 15.633773107649876 14.814988132651957 7 30.952950706634546 31.10925582244533 19.007326026804787 18.930467444115337 8 28.099448375615083 34.198088458429645 18.183078319318195 19.519384846637077 9 26.91209654118652 15.709709954569062 18.47236476629567 38.90582873794875 10-11 26.61742470570927 25.11843418363458 25.87112006849206 22.393021042164094 12-13 27.156852839566568 22.19731172217466 26.619356805689236 24.026478632569535 14-15 23.52388967788091 23.855201332407812 24.321813498357532 28.299095491353743 16-17 23.76204682063199 26.961054891137685 25.257953073013883 24.01894521521644 18-19 23.919356988692282 25.37401692045458 27.19982217356654 23.5068039172866 20-21 24.217951513153345 24.625589157551232 27.41610875064034 23.740350578655082 22-23 23.94660668293885 24.488906378234272 27.581507144338502 23.982979794488372 24-25 23.991009531102378 24.850864924940574 27.401485058131392 23.756640485825653 26-27 23.542645568821474 25.122041490916285 27.331267224955525 24.004045715306717 28-29 23.592353960113474 25.643120046002732 27.111353840285147 23.653172153598643 30-31 23.609274869040338 25.205963428794288 27.018586965192647 24.166174736972724 32-33 23.429641961486947 25.137888468276966 27.13015437694407 24.302315193292014 34-35 24.049974811583198 25.25134352994479 26.939658763424223 23.759022895047792 36-37 24.19434079850076 24.83022306852866 27.02376424928571 23.95167188368487 38-39 23.777829114948553 25.040587588787844 27.09524063648715 24.086342659776456 40-41 23.871021732778456 25.056115588538162 27.22047522190923 23.852387456774153 42-43 23.818724036502704 25.098783184075547 26.824789499532812 24.25770327988894 44-45 23.68723259508848 25.159177452696223 27.18460648404662 23.968983468168677 46-47 24.16550989973229 25.42912669696335 26.661325060048746 23.744038343255614 48-49 23.911419211759423 25.656825775300867 26.305796946243298 24.125958066696406 50-51 23.787111874115404 25.761784394631682 26.591938658331856 23.859165072921066 52-53 24.244247119238143 25.316203448847475 26.460849207552258 23.97870022436213 54-55 23.632375732489656 25.564637968306737 26.60319811711112 24.199788182092487 56-57 23.56541872407418 25.63463780100403 26.71294598268955 24.08699749223224 58-59 22.00865218515719 25.108536308315273 28.47680534262253 24.406006163905005 60-61 23.187269385815927 25.45573416745612 27.199280526419106 24.157715920308842 62-63 23.348292097025812 25.522674872981675 26.874848555590358 24.254184474402148 64-65 22.5591122004628 25.582743589980268 27.266006144644102 24.592138064912834 66-67 22.343274772529057 25.24762505935217 28.297836497793384 24.111263670325393 68-69 22.85629966981669 24.232685870431517 29.3502630080838 23.560751451667993 70-71 21.99733163390945 24.64966143497067 29.163831625846004 24.189175305273874 72-73 21.83752252237611 24.9146145075703 28.999087367497644 24.24877560255595 74-75 22.428209776509007 25.211832403628705 28.16312773966998 24.196830080192314 76-77 21.195920365424094 25.377224950092607 28.590692712974203 24.836161971509096 78-79 22.316342094616015 25.67943506004703 27.231104662805162 24.773118182531796 80-81 23.163447736266914 25.702425692924713 26.209966033988703 24.924160536819674 82-83 23.25079880982388 25.76194917750861 26.018741764184742 24.968510248482765 84-85 22.96227192349307 25.920867538014527 25.946250732071167 25.17060980642124 86-87 23.248351642118266 25.75715623787972 25.91720595628156 25.07728616372046 88-89 23.046429434289887 25.81603320660055 25.61302086721525 25.524516491894307 90-91 22.567126389530166 25.756625855773642 26.148082837875698 25.5281649168205 92-93 22.448105894676022 25.602849863158767 26.380837504210536 25.56820673795468 94-95 20.811792454862914 25.1553349316318 28.32372941735844 25.709143196146844 96-97 20.76053326727181 24.807178445992463 28.442177768323322 25.990110518412408 98-99 21.037075734095666 25.278248826277633 27.454128867411626 26.230546572215076 100-101 21.07801664811695 25.14609902522346 27.329549357605636 26.446334969053954 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 326.0 1 260.0 2 218.5 3 257.5 4 311.0 5 375.5 6 478.5 7 692.5 8 985.5 9 1314.5 10 1521.0 11 1640.5 12 1816.0 13 1983.0 14 2232.0 15 2576.5 16 2825.5 17 3011.5 18 3279.5 19 3656.0 20 4152.5 21 4807.5 22 5621.5 23 6637.0 24 7852.5 25 9496.5 26 11538.5 27 13686.0 28 16028.5 29 18522.5 30 21433.5 31 25397.5 32 29952.5 33 33019.5 34 34851.0 35 37501.0 36 41079.0 37 45038.5 38 49609.5 39 54316.0 40 59376.0 41 65651.5 42 72221.0 43 78722.0 44 85626.0 45 92995.0 46 100218.0 47 107032.0 48 113172.5 49 117306.0 50 120056.0 51 123141.5 52 125821.5 53 127920.0 54 127293.0 55 122140.5 56 114319.0 57 104354.0 58 92975.5 59 80268.0 60 67123.0 61 55019.5 62 43487.5 63 32944.5 64 24466.0 65 18105.0 66 13355.5 67 9907.0 68 7330.5 69 5302.0 70 3760.0 71 2681.5 72 1973.5 73 1485.0 74 1089.5 75 742.0 76 475.5 77 301.0 78 193.0 79 115.5 80 64.5 81 40.5 82 24.5 83 11.5 84 8.0 85 5.5 86 3.0 87 1.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02151898509801419 2 0.005034095360655708 3 0.003261526571692431 4 0.0 5 0.061720845231701335 6 0.0 7 0.0 8 1.7725687889632777E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.4815963045485886E-4 16-17 0.0 18-19 0.0013117009038328255 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0635412733779666E-4 28-29 0.0022334366740937297 30-31 0.008508330187023733 32-33 2.4815963045485886E-4 34-35 8.508330187023733E-4 36-37 0.02366379333265976 38-39 0.022316641053047665 40-41 0.02456780341503103 42-43 0.024815963045485886 44-45 0.02554271624896083 46-47 0.027102576783248514 48-49 0.027049399719579616 50-51 0.02221028692570987 52-53 0.01940962823914789 54-55 0.014818675075733003 56-57 0.15300813786331013 58-59 0.7530226729273796 60-61 0.3087460316616237 62-63 3.722394456822883E-4 64-65 0.14191185724440003 66-67 1.4072600872458358 68-69 2.6970520408470753 70-71 2.176661295783236 72-73 1.8382247369064777 74-75 1.5466903481856873 76-77 1.1113474536163062 78-79 0.416855002100494 80-81 1.4180550311706223E-4 82-83 2.1270825467559331E-4 84-85 3.545137577926556E-5 86-87 0.002800658686561979 88-89 1.772568788963278E-5 90-91 0.22261691420589805 92-93 0.1740839807640835 94-95 1.2811063665353193 96-97 1.25790344108779 98-99 0.9905468906484588 100-101 0.39698450597621565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2820765.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.2674397554234 #Duplication Level Percentage of deduplicated Percentage of total 1 71.92128069776783 32.55692241119102 2 14.103250207874035 12.768360582812011 3 5.225881400301626 7.0968681437142465 4 2.609122757739282 4.724332290018685 5 1.460709386180761 3.306128706955955 6 0.9509865512322906 2.5829235849675354 7 0.6404556968820493 2.0294252772238117 8 0.4668489958308727 1.6906447034923153 9 0.36943285419526656 1.5050951523862515 >10 2.025780131457385 16.862725574269568 >50 0.14440465606901973 4.519547846994478 >100 0.07423891934632168 5.956625723679608 >500 0.004392100277402646 1.344478895037417 >1k 0.0030587841217376366 2.5258905961824247 >5k 1.5686072419167366E-4 0.5300305110746704 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8162 0.28935412911036545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6767 0.23989945989829 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4330 0.15350445712421984 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3637 0.12893665370918883 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3441 0.12198818405645276 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3265 0.11574874191930204 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2859 0.10135548335292022 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7725687889632777E-4 2 3.545137577926556E-5 0.0 0.0 0.0 1.7725687889632777E-4 3 3.545137577926556E-5 0.0 0.0 0.0 1.7725687889632777E-4 4 3.545137577926556E-5 0.0 0.0 0.0 1.7725687889632777E-4 5 7.090275155853111E-5 0.0 0.0 0.0 1.7725687889632777E-4 6 7.090275155853111E-5 0.0 0.0 0.0 1.7725687889632777E-4 7 1.4180550311706223E-4 0.0 0.0 0.0 1.7725687889632777E-4 8 1.4180550311706223E-4 0.0 0.0 0.0 1.7725687889632777E-4 9 1.4180550311706223E-4 0.0 0.0 0.0 2.1270825467559331E-4 10-11 1.4180550311706223E-4 0.0 0.0 1.772568788963278E-5 2.1270825467559331E-4 12-13 1.7725687889632777E-4 0.0 0.0 3.545137577926556E-5 8.153816429231078E-4 14-15 2.8361100623412446E-4 0.0 0.0 7.090275155853111E-5 9.749128339298028E-4 16-17 2.8361100623412446E-4 0.0 0.0 1.2407981522742943E-4 0.0010812669612675994 18-19 3.7223944568228834E-4 0.0 0.0 1.4180550311706223E-4 0.0011698954007157633 20-21 4.608678851304522E-4 0.0 0.0 2.4815963045485886E-4 0.0012230724643846615 22-23 4.963192609097177E-4 0.0 0.0 5.494963245786162E-4 0.0013117009038328255 24-25 7.444788913645767E-4 0.0 0.0 9.217357702609043E-4 0.0013117009038328255 26-27 7.444788913645767E-4 0.0 0.0 0.0021270825467559333 0.0013648779675017237 28-29 0.0010989926491572323 0.0 0.0 0.00650532745549523 0.0013826036553913567 30-31 0.0013117009038328255 0.0 0.0 0.024479174975582864 0.0014003293432809894 32-33 0.0013294265917224582 0.0 0.0 0.06324525439020975 0.0015244091585084189 34-35 0.0014712320948395206 0.0 0.0 0.11737950520514825 0.0016484889737358483 36-37 0.0015953119100669498 0.0 0.0 0.21425038952199138 0.001666214661625481 38-39 0.0017371174131840122 0.0 0.0 0.36545050722055894 0.001754843101073645 40-41 0.001754843101073645 0.0 0.0 0.5241840422722205 0.0017725687889632777 42-43 0.0018789229163010744 0.0 0.0 0.6808968489044638 0.0017902944768529105 44-45 0.001985277043638871 0.0 0.0 0.8719797643547051 0.001896648604190707 46-47 0.0021093568588663007 0.0 0.0 1.0971314519288207 0.002020728419418137 48-49 0.0021270825467559333 0.0 0.0 1.3193584010011468 0.0020916311709766678 50-51 0.0021270825467559333 0.0 0.0 1.5455736298486404 0.0021802596104248317 52-53 0.0021270825467559333 0.0 0.0 1.756668846926277 0.0022866137377626286 54-55 0.0021625339225351987 0.0 0.0 1.981607826245717 0.0023575164893211596 56-57 0.0022334366740937297 0.0 0.0 2.1827412067293803 0.0024106935529900576 58-59 0.0022334366740937297 0.0 0.0 2.317952753951499 0.0024106935529900576 60-61 0.0022334366740937297 0.0 0.0 2.422445684060884 0.0024106935529900576 62-63 0.0022511623619833627 0.0 0.0 2.513112577616356 0.0024284192408796906 64-65 0.0022866137377626286 3.545137577926556E-5 0.0 2.6015105831219545 0.002446144928769323 66-67 0.0023397908014315266 3.545137577926556E-5 0.0 2.682570153841245 0.002446144928769323 68-69 0.0023397908014315266 3.545137577926556E-5 0.0 2.7754173070071415 0.002446144928769323 70-71 0.0023929678651004246 3.545137577926556E-5 0.0 2.9404789126354025 0.002446144928769323 72-73 0.0024638706166589556 3.545137577926556E-5 0.0 3.1482239747018985 0.0025524990561071196 74-75 0.0024815963045485886 3.545137577926556E-5 0.0 3.3795973787252747 0.002694304559224182 76-77 0.00255249905610712 3.545137577926556E-5 0.0 3.718955673372294 0.0027297559350034474 78-79 0.0025879504318863855 3.545137577926556E-5 0.0 4.206146205018851 0.0027474816228930804 80-81 0.0027829329986723463 3.545137577926556E-5 0.0 4.734158995875232 0.002800658686561979 82-83 0.0028361100623412443 3.545137577926556E-5 0.0 5.238720701653629 0.003084269692796103 84-85 0.0028538357502308773 3.545137577926556E-5 0.0 5.648166366216257 0.003137446756465001 86-87 0.00287156143812051 3.545137577926556E-5 0.0 6.009912204667883 0.0031906238201339 88-89 0.00287156143812051 3.545137577926556E-5 0.0 6.383871042075466 0.003243800883802798 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5580 0.0 43.032806 1 GTATCAA 9245 0.0 35.566566 1 TCAACGC 11460 0.0 28.476799 4 ATCAACG 11465 0.0 28.425768 3 TATCAAC 11625 0.0 28.156744 2 CAACGCA 11670 0.0 28.045187 5 AACGCAG 12110 0.0 27.050276 6 TAAGGTG 1525 0.0 24.739439 5 GTACATG 10655 0.0 24.395754 1 TACATGG 10485 0.0 24.29077 2 AGGTAAG 1270 0.0 23.767975 2 ACATGGG 10380 0.0 23.445559 3 GGTAAGG 1450 0.0 23.419237 3 GTAAGGT 1565 0.0 23.203106 4 ACGCAGA 14105 0.0 23.157473 7 CGCAGAG 14135 0.0 23.074978 8 GAGTACA 7725 0.0 20.824373 1 GCAGAGT 15645 0.0 20.606846 9 GAGTACT 8755 0.0 20.538897 12-13 GTACTTT 8920 0.0 20.15933 14-15 >>END_MODULE