FastQCFastQC Report
Fri 27 May 2016
SRR939880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987283
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73840.247181134161042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56170.18803039417423792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.11813410379933874No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31920.10685294965358154No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31550.10561436596398802No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30770.10300329764538546No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30550.10226684247860011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.038.8094671
GTATCAA96350.035.8799931
ATCAACG119250.028.8335133
TATCAAC118900.028.7992252
TCAACGC120550.028.562554
CAACGCA122200.027.8313835
AACGCAG126750.026.7214166
GTACATG107600.025.3094671
TACATGG106400.025.1094952
ACATGGG108900.023.4536553
ACGCAGA145000.023.0662277
CGCAGAG147400.022.626458
GTATAGG5250.022.3973921
TAAGGTG13300.022.2821245
GAGTACT79000.020.12464312-13
GCAGAGT157400.020.1130569
GAGTACA73900.019.9212531
AGAGTAC122250.019.7764910-11
TATAGGG6000.019.5983732
CATGGGG71900.019.299514
Produced by FastQC (version 0.11.5)