FastQCFastQC Report
Fri 27 May 2016
SRR939880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987283
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.28437212008370144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70870.23723898940943997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46850.15683147529042277No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37940.12700504103561663No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36440.1219837558075348No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34570.11572388688985945No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA29900.10009095221309798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60150.040.0076871
GTATCAA97550.035.2762031
TCAACGC122700.027.6043514
ATCAACG123200.027.492323
TATCAAC124750.027.3486442
CAACGCA125600.026.9669885
AACGCAG130150.026.0971286
TACATGG111350.023.9919552
GTACATG114650.023.5150221
ACGCAGA149550.022.5848627
CGCAGAG151800.022.1876058
ACATGGG114100.022.1182043
GAGTACA81800.020.3668981
TAAGGTG14300.020.2356745
AGAGTAC133550.019.98032210-11
GAGTACT88700.019.94844812-13
GCAGAGT167150.019.7810929
GTACTTT91800.019.09394314-15
AGGTAAG12550.018.9048712
AGTACTT94900.018.79513712-13
Produced by FastQC (version 0.11.5)