##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939880_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2987283 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.357421442829487 31.0 31.0 34.0 30.0 34.0 2 31.5873353813482 31.0 31.0 34.0 30.0 34.0 3 31.63119296029201 31.0 31.0 34.0 30.0 34.0 4 35.44943549037704 37.0 35.0 37.0 33.0 37.0 5 35.164745690314575 37.0 35.0 37.0 32.0 37.0 6 35.194898508109205 37.0 35.0 37.0 32.0 37.0 7 35.0935157465831 37.0 35.0 37.0 32.0 37.0 8 35.048090522391085 37.0 35.0 37.0 32.0 37.0 9 36.6394666993385 39.0 35.0 39.0 32.0 39.0 10-11 36.49492900404816 38.0 35.0 39.0 32.0 39.0 12-13 36.406607274904985 38.0 35.0 39.0 32.0 39.0 14-15 37.44178020629448 39.0 36.0 41.0 32.0 41.0 16-17 37.348532596342565 39.0 36.0 41.0 32.0 41.0 18-19 37.29974160466216 39.0 36.0 41.0 31.5 41.0 20-21 37.19369239539742 39.0 36.0 40.0 31.0 41.0 22-23 37.06633536226732 39.0 36.0 40.0 31.0 41.0 24-25 36.8943883120548 39.0 36.0 40.0 30.5 41.0 26-27 36.57555628308399 38.0 35.0 40.0 30.0 41.0 28-29 36.34280397940202 38.0 35.0 40.0 30.0 41.0 30-31 36.05903508305039 38.0 34.5 40.0 29.5 41.0 32-33 36.02818698462784 38.0 35.0 40.0 29.5 41.0 34-35 35.873593998292094 38.0 34.0 40.0 29.0 41.0 36-37 35.935014359201986 38.0 35.0 40.0 29.0 41.0 38-39 35.82164495295558 38.0 34.0 40.0 29.0 41.0 40-41 35.72246268599259 38.0 34.0 40.0 28.0 41.0 42-43 35.47537511511297 38.0 34.0 40.0 27.0 41.0 44-45 35.21594070598601 38.0 34.0 40.0 27.0 41.0 46-47 34.92162744540775 38.0 33.0 40.0 26.0 41.0 48-49 34.58310059676301 38.0 33.0 40.0 25.0 41.0 50-51 34.314846132756756 37.5 33.0 40.0 24.0 41.0 52-53 34.026944551286235 37.0 33.0 40.0 24.0 41.0 54-55 33.713977383461824 37.0 32.0 40.0 23.0 41.0 56-57 33.295268643780986 36.0 31.5 39.0 22.5 41.0 58-59 32.87148489112012 36.0 31.0 39.0 22.0 40.0 60-61 32.51321317732535 35.0 31.0 39.0 21.0 40.0 62-63 32.17442840199607 35.0 31.0 38.5 20.0 40.0 64-65 31.686416050973406 35.0 30.0 38.0 18.5 40.0 66-67 30.74919215889489 34.0 29.0 37.0 12.5 39.5 68-69 28.85710426497925 33.0 26.0 36.0 7.5 39.0 70-71 28.182469655536487 33.0 25.0 36.0 7.5 39.0 72-73 28.727761146165264 33.0 26.0 35.0 7.5 38.0 74-75 28.831831466921614 33.0 26.5 35.0 7.0 37.5 76-77 28.434063495155968 32.5 26.5 34.5 7.0 36.0 78-79 28.982969306891917 33.0 28.0 35.0 7.0 36.5 80-81 28.82993074308661 33.0 28.0 35.0 3.5 36.0 82-83 28.419986991523736 33.0 27.5 35.0 2.0 36.0 84-85 28.08950708720935 33.0 27.0 35.0 2.0 35.0 86-87 27.727882828643956 33.0 26.0 35.0 2.0 35.0 88-89 27.275623869583164 32.5 25.5 35.0 2.0 35.0 90-91 26.7913194029491 32.0 25.0 34.0 2.0 35.0 92-93 26.29914524335324 32.0 24.5 34.0 2.0 35.0 94-95 25.48661894437186 32.0 21.5 34.0 2.0 35.0 96-97 24.92551994571656 31.0 19.5 34.0 2.0 35.0 98-99 24.225688694375457 31.0 10.0 34.0 2.0 35.0 100-101 22.722235389147933 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30054120854383726 1101 2 0.33292816282821036 1101 3 0.3634794007486839 1101 4 0.2243978264562827 1101 5 0.34792513701330563 1101 6 0.3471626674717996 1101 7 0.33262546125898496 1101 8 0.33454741189522963 1101 9 0.4092129561098048 1101 10-11 0.37341637965030827 1101 12-13 0.37963780828132343 1101 14-15 0.48289359270351895 1101 16-17 0.5108372045754237 1101 18-19 0.45512901592832833 1101 20-21 0.457648261567563 1101 22-23 0.4683680611911427 1101 24-25 0.5288896442430868 1101 26-27 0.36432158096315703 1101 28-29 0.38201517831417675 1101 30-31 0.31050215124207625 1101 32-33 0.23181939690005748 1101 34-35 0.4185098987946745 1101 36-37 0.5869653487212929 1101 38-39 0.5680408962313592 1101 40-41 0.5724159113371812 1101 42-43 0.4589303080885614 1101 44-45 0.3723649248689327 1101 46-47 0.2725772958730417 1101 48-49 0.31924507198676366 1101 50-51 0.3028878159485231 1101 52-53 0.20111632336999463 1101 54-55 0.09671165956667238 1101 56-57 0.015506465108536815 1101 58-59 0.02757798631552788 1101 60-61 0.004557231585671673 1101 62-63 0.0744026538151843 1101 64-65 -0.19828570300229487 1101 66-67 -0.1879672733909814 1101 68-69 -8.561853518473665 1101 70-71 -6.911108798578152 1101 72-73 -1.8150275944711574 1101 74-75 -0.693827500306039 1101 76-77 -0.6561386707639016 1101 78-79 -0.654564268219243 1101 80-81 -0.5349858393673301 1101 82-83 -0.4923016044367152 1101 84-85 -0.3141229402543573 1101 86-87 -0.4210782714113712 1101 88-89 -0.4255533069519899 1101 90-91 -0.7382861755772296 1101 92-93 -1.0310471990447745 1101 94-95 -0.7328771759345614 1101 96-97 -1.1033831895589294 1101 98-99 -0.9350002322257644 1101 100-101 -1.0116283579450531 1102 1 0.16523796371189547 1102 2 0.1353365113055638 1102 3 0.2319662885325222 1102 4 0.13224856685522468 1102 5 0.34228941596177265 1102 6 0.2026187194624285 1102 7 0.13679111526306542 1102 8 0.05751726751613262 1102 9 0.15436618380140033 1102 10-11 0.20906074917866846 1102 12-13 0.2771064901803655 1102 14-15 0.24869707448400646 1102 16-17 0.28067385799191413 1102 18-19 0.31212885873602403 1102 20-21 0.24994096639463947 1102 22-23 0.21860527075516245 1102 24-25 0.2189275386087033 1102 26-27 0.2142578629765879 1102 28-29 0.11339470607601498 1102 30-31 -0.01811288017028545 1102 32-33 -0.1348501506587354 1102 34-35 -0.09438300220376306 1102 36-37 -0.09596310325935065 1102 38-39 0.11902920657652061 1102 40-41 0.07034439522033864 1102 42-43 -0.11731097455422201 1102 44-45 -0.09205665676404351 1102 46-47 -0.21286855311910102 1102 48-49 -0.09167196238034592 1102 50-51 -0.22689815310141626 1102 52-53 -0.25056311106254725 1102 54-55 -0.5357300473213158 1102 56-57 -0.5522756675211653 1102 58-59 -0.6342984141315711 1102 60-61 -0.7103673944155631 1102 62-63 -0.6228570957328117 1102 64-65 -0.6031517038155201 1102 66-67 -0.42886604212862167 1102 68-69 -5.238632213174309 1102 70-71 -0.5059925081772647 1102 72-73 -0.10963270826736249 1102 74-75 -0.31120513827998764 1102 76-77 -0.535590736682579 1102 78-79 -0.7005463807793753 1102 80-81 -0.6246709501241021 1102 82-83 -0.6162130477462675 1102 84-85 -0.5937233237803738 1102 86-87 -0.5579937524169161 1102 88-89 -0.6501400391096475 1102 90-91 -0.7528571479015227 1102 92-93 -0.8785291929856207 1102 94-95 -0.6603755039537091 1102 96-97 -1.1213272998650048 1102 98-99 -1.0321827777317765 1102 100-101 -1.0452397263707418 1103 1 0.18098753436054338 1103 2 0.22676255395866818 1103 3 0.17820462037927243 1103 4 0.17947297373126503 1103 5 0.21991155467034673 1103 6 0.1272671557980516 1103 7 0.007741970870313253 1103 8 0.18392014010024127 1103 9 0.2306666770108876 1103 10-11 0.2900348092488798 1103 12-13 0.3512880857394194 1103 14-15 0.32951481397493154 1103 16-17 0.3644461538809196 1103 18-19 0.31683951375766384 1103 20-21 0.2856977368470126 1103 22-23 0.19728210045688144 1103 24-25 0.1787044822690902 1103 26-27 0.13057395958235674 1103 28-29 0.152062237942026 1103 30-31 0.21388520293929503 1103 32-33 0.20539251024197824 1103 34-35 0.13135130898826475 1103 36-37 0.24112465638643243 1103 38-39 0.20308009359693102 1103 40-41 0.29319735184036944 1103 42-43 0.16857867125671078 1103 44-45 0.26945047539820877 1103 46-47 0.11116789989988263 1103 48-49 0.08247019476339545 1103 50-51 0.031913237263232475 1103 52-53 0.07842429615973145 1103 54-55 -0.04032244442029054 1103 56-57 0.13920036103041156 1103 58-59 0.01600878131558403 1103 60-61 0.003950470040102516 1103 62-63 -0.3007600038573557 1103 64-65 -0.37241886009941183 1103 66-67 -0.04576230763170486 1103 68-69 -4.148223887999986 1103 70-71 0.27804783135997724 1103 72-73 0.5774280521144561 1103 74-75 0.306764448281605 1103 76-77 0.10462693126889278 1103 78-79 -0.031910697726658555 1103 80-81 -0.09226042602883311 1103 82-83 -0.2230695423590241 1103 84-85 -0.14648384088220112 1103 86-87 -0.2411042510606478 1103 88-89 -0.17434548115237547 1103 90-91 -0.29722123784862475 1103 92-93 -0.3743582939502552 1103 94-95 -0.31464327932900815 1103 96-97 -0.6515788573384675 1103 98-99 -0.41345165142108087 1103 100-101 -0.5050881521588799 1104 1 0.1808425890005516 1104 2 0.22243395843528546 1104 3 0.2095602548007207 1104 4 0.17638695524853887 1104 5 0.20080160702637073 1104 6 0.18022140577169665 1104 7 0.1496882795951464 1104 8 0.15850506929663055 1104 9 0.30071876949480725 1104 10-11 0.2460467474757948 1104 12-13 0.24097829797456427 1104 14-15 0.25829437404379973 1104 16-17 0.2737604305596477 1104 18-19 0.24817406521766117 1104 20-21 0.3515450962060882 1104 22-23 0.26145599975059497 1104 24-25 0.2089499670015087 1104 26-27 0.32719519539528363 1104 28-29 0.25858741451182965 1104 30-31 0.1923836568554549 1104 32-33 0.19227671207619323 1104 34-35 0.13482143267497548 1104 36-37 0.2044374484732927 1104 38-39 0.16500953595259915 1104 40-41 0.13147149183124185 1104 42-43 0.01673215521608995 1104 44-45 0.13983835796611999 1104 46-47 0.09711918068979486 1104 48-49 0.182405188965582 1104 50-51 0.14458124211230938 1104 52-53 0.018084640866142365 1104 54-55 0.04434497186277753 1104 56-57 0.14376438295668947 1104 58-59 0.0021118155051240706 1104 60-61 -0.07906940065106483 1104 62-63 0.17404453952083543 1104 64-65 0.13507942690997155 1104 66-67 0.12306543578865714 1104 68-69 -4.09594187703685 1104 70-71 0.6139681465502491 1104 72-73 0.8250759842273183 1104 74-75 0.5962510781702122 1104 76-77 0.4980268662630962 1104 78-79 0.10526976363881602 1104 80-81 -0.0634103698076025 1104 82-83 -0.022788875434681444 1104 84-85 0.1341723223701976 1104 86-87 0.2083561568946557 1104 88-89 0.2889710443755753 1104 90-91 0.0824501754125535 1104 92-93 -0.06420860492394098 1104 94-95 0.33680222121563475 1104 96-97 0.11852067180872794 1104 98-99 0.17520769184467255 1104 100-101 0.21222513186375025 1105 1 0.16930310047199626 1105 2 0.197409768030397 1105 3 0.15903429057590657 1105 4 0.14209346102933296 1105 5 0.07048551334532505 1105 6 0.17028015819073516 1105 7 0.11188900114319722 1105 8 0.21345474381011798 1105 9 0.3311071338885938 1105 10-11 0.21597256857584313 1105 12-13 0.18690345968752808 1105 14-15 0.217802920318519 1105 16-17 0.30409481192921817 1105 18-19 0.24078536683745 1105 20-21 0.1745820888515155 1105 22-23 0.27454843929390194 1105 24-25 0.2447882020936092 1105 26-27 0.3106172018380491 1105 28-29 0.27688454738004253 1105 30-31 0.28216944620533013 1105 32-33 0.20902765522294686 1105 34-35 0.2629161195433838 1105 36-37 0.055878365931427254 1105 38-39 0.20256132960565054 1105 40-41 0.17952910105510256 1105 42-43 0.1985800359906591 1105 44-45 0.2570929745342525 1105 46-47 0.21655892919543618 1105 48-49 0.20236581462776115 1105 50-51 0.22699931455559152 1105 52-53 0.2533890772189764 1105 54-55 0.32014220346733424 1105 56-57 0.2420183165268739 1105 58-59 0.2763384416068888 1105 60-61 0.2750061137653006 1105 62-63 0.3519699042872446 1105 64-65 0.10960121029444636 1105 66-67 0.7723022574914182 1105 68-69 2.0001677069998003 1105 70-71 2.1470406907197415 1105 72-73 1.3364820713660102 1105 74-75 0.7922096707222295 1105 76-77 0.5227769023233257 1105 78-79 0.4956001376005794 1105 80-81 0.469366501933024 1105 82-83 0.4794493272890712 1105 84-85 0.23998575746779238 1105 86-87 0.24759575180089044 1105 88-89 0.336718439023894 1105 90-91 0.31982979219532837 1105 92-93 0.31892727002604815 1105 94-95 0.55845533810745 1105 96-97 0.13094374331616265 1105 98-99 0.352455478433356 1105 100-101 0.375776792008093 1106 1 0.33015822354877855 1106 2 0.35100214376388195 1106 3 0.34075522904880273 1106 4 0.34792149797222294 1106 5 0.3712849015675985 1106 6 0.42474679284939754 1106 7 0.36187885267076325 1106 8 0.34825203738768806 1106 9 0.441008028077448 1106 10-11 0.37035085313589633 1106 12-13 0.3905913645212209 1106 14-15 0.5614696229079996 1106 16-17 0.5015073462769308 1106 18-19 0.4379715207398931 1106 20-21 0.5216781966092796 1106 22-23 0.4372694354879769 1106 24-25 0.46218221673031934 1106 26-27 0.594455072734128 1106 28-29 0.5588737836599478 1106 30-31 0.7535416898385066 1106 32-33 0.6634716907627336 1106 34-35 0.6782015096022818 1106 36-37 0.7440944379754626 1106 38-39 0.8899773127657511 1106 40-41 0.6121893432104386 1106 42-43 0.5092040908620348 1106 44-45 0.694723688249347 1106 46-47 0.6015701748150519 1106 48-49 0.5952334793257918 1106 50-51 0.7131913863798687 1106 52-53 0.7172284295140017 1106 54-55 0.8117681288829104 1106 56-57 0.7658473190482553 1106 58-59 0.7810207521577084 1106 60-61 0.8487103818291146 1106 62-63 0.9848716856472208 1106 64-65 0.9646541096935586 1106 66-67 1.4969436716446332 1106 68-69 2.9773071189845197 1106 70-71 3.0933164015270904 1106 72-73 2.210541085539507 1106 74-75 1.5993087301562454 1106 76-77 1.1134183313794992 1106 78-79 1.0496359987988377 1106 80-81 1.1280455700886094 1106 82-83 1.1393580010466557 1106 84-85 1.1090254205896706 1106 86-87 1.1534292160912258 1106 88-89 1.1763543351885062 1106 90-91 1.080703254812974 1106 92-93 1.150847213092284 1106 94-95 1.3510335739325932 1106 96-97 1.2322124858764987 1106 98-99 1.5344020744466356 1106 100-101 1.6279391395405014 1107 1 0.08794848757135298 1107 2 0.04986026737904936 1107 3 0.0736616148119289 1107 4 0.134560297437595 1107 5 0.08519070894850955 1107 6 0.010132442047307677 1107 7 0.07808272954559214 1107 8 -0.015467030842259533 1107 9 -0.08132051654686734 1107 10-11 0.10679105006143175 1107 12-13 -0.023255562810653885 1107 14-15 0.1873946227651686 1107 16-17 0.1322339939688959 1107 18-19 -0.004070783499905417 1107 20-21 -0.021325075736825738 1107 22-23 0.020134870714713315 1107 24-25 0.22586216602357467 1107 26-27 0.11964208914559293 1107 28-29 0.04906128233665896 1107 30-31 0.1861988100059051 1107 32-33 -0.06539778204910363 1107 34-35 0.016470415996110432 1107 36-37 -0.022743760523567857 1107 38-39 0.15641390362434748 1107 40-41 0.2019940207463904 1107 42-43 0.18994332450056106 1107 44-45 0.22718417190592533 1107 46-47 0.1292340517369297 1107 48-49 0.2401209132411637 1107 50-51 0.0881363123613923 1107 52-53 0.04792812412415515 1107 54-55 -0.0754451543486141 1107 56-57 -0.038183275724257726 1107 58-59 0.0839888510681206 1107 60-61 0.2830860130168489 1107 62-63 0.41284007862181227 1107 64-65 0.2761757791983648 1107 66-67 0.7649394747607694 1107 68-69 2.1023971446302774 1107 70-71 2.3832497279373115 1107 72-73 1.305303348939674 1107 74-75 0.8015158245972245 1107 76-77 0.4284116384789485 1107 78-79 0.47000627768257885 1107 80-81 0.4288338973205299 1107 82-83 0.5522691321653319 1107 84-85 0.4424752033350572 1107 86-87 0.4452292618644158 1107 88-89 0.4851753193389534 1107 90-91 0.3802623938065217 1107 92-93 0.4002662263070249 1107 94-95 0.8056204877072624 1107 96-97 0.5430551191993125 1107 98-99 0.73273407524918 1107 100-101 0.8420991864025673 1108 1 -0.17169017876593173 1108 2 -0.083303676182787 1108 3 -0.14962971808171943 1108 4 -0.1403568433126594 1108 5 -0.003927760443254158 1108 6 0.03576934247556096 1108 7 -0.057835592647201395 1108 8 -0.03965619914305307 1108 9 -0.03709447018267298 1108 10-11 -0.06975960549721805 1108 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-0.22505149546222825 2316 84-85 -0.3252233589642408 2316 86-87 -0.4614740495053731 2316 88-89 -0.3803072616886496 2316 90-91 -0.4256739076093723 2316 92-93 -0.4430779823841 2316 94-95 -0.006577820190649675 2316 96-97 -0.08320235387413888 2316 98-99 -0.11890336697318915 2316 100-101 -0.23366760600223202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 28.0 4 73.0 5 128.0 6 396.0 7 951.0 8 2162.0 9 3443.0 10 5567.0 11 8228.0 12 11105.0 13 14023.0 14 17254.0 15 20981.0 16 25156.0 17 29561.0 18 28099.0 19 25978.0 20 25186.0 21 26305.0 22 28357.0 23 32583.0 24 38967.0 25 47429.0 26 58960.0 27 72919.0 28 88829.0 29 106549.0 30 125609.0 31 148941.0 32 177677.0 33 215469.0 34 267293.0 35 335486.0 36 408743.0 37 399226.0 38 178598.0 39 11020.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.891937681853335 18.03422609580762 13.610381000532376 24.46345522180666 2 17.064779564654003 19.694166256499887 37.39971003719962 25.841344141646495 3 20.47378838898089 22.604754889309113 28.732731381660194 28.188725340049803 4 14.225334526390704 15.535889970920064 33.89387614096154 36.3448993617277 5 14.771181705918055 36.43605242623481 33.19009280339358 15.602673064453551 6 35.56718113656495 33.95333952402217 15.624135920211083 14.855343419201802 7 30.878058757740728 31.190315748457714 18.979453905103735 18.952171588697823 8 28.062490229415825 34.23157431016747 18.181303880482698 19.524631579934006 9 26.88874806973427 15.722614830935001 18.484790359667965 38.90384673966276 10-11 26.639139981046323 25.12150673371087 25.881227188719645 22.35812609652316 12-13 27.17070997290849 22.163032427794754 26.606702478472915 24.059555120823838 14-15 23.514695460724678 23.87453749778645 24.318837552384558 28.291929489104312 16-17 23.765275002347458 26.97222836401543 25.23502610824683 24.027470525390285 18-19 23.93224880267454 25.32127019770139 27.21026096288835 23.53622003673572 20-21 24.25299176542698 24.63650079353044 27.432118081882432 23.678389359160146 22-23 23.935094197637117 24.52325407401977 27.562035468350338 23.979616259992774 24-25 24.014684928315237 24.85913190944071 27.33094531966449 23.795237842579564 26-27 23.52212595268761 25.15222498114919 27.2925553326655 24.033093733497697 28-29 23.564358954574235 25.64394270976562 27.143785124965667 23.647913210694476 30-31 23.60354986206474 25.164807612031183 27.050876870561797 24.180765655342285 32-33 23.425479781448935 25.139615619140137 27.10075372767217 24.334150871738753 34-35 24.065529789912528 25.248308552553507 26.973391620570773 23.712770036963196 36-37 24.236287112116216 24.854770025384354 27.002764617784596 23.90617824471483 38-39 23.78123280201237 25.059619690455072 27.062573241962546 24.096574265570016 40-41 23.889990491867824 25.06087298894627 27.21247533959111 23.836661179594795 42-43 23.7681884484608 25.123440014770914 26.84121232754839 24.267159209219894 44-45 23.696131657182683 25.15732131982204 27.165351362535574 23.981195660459704 46-47 24.15648127378125 25.467382758741426 26.634698390133032 23.741437577344293 48-49 23.933745795703302 25.64228552914457 26.350605969887713 24.073362705264415 50-51 23.781637291450316 25.79438225267751 26.582117953964485 23.84186250190769 52-53 24.242882058753132 25.327756013822256 26.477131823131263 23.952230104293353 54-55 23.64257607080604 25.5863491238342 26.61893721269546 24.152137592664296 56-57 23.65417306181248 25.701127593895418 26.397119258537572 24.24758008575453 58-59 23.65779875933109 25.342345706933024 26.38693891052349 24.612916623212396 60-61 23.246580506409124 25.569887699732707 26.86316200378173 24.320369790076438 62-63 23.409383899360712 25.507637026094137 26.809414630243538 24.273564444301616 64-65 23.108872989184427 25.64831933019264 26.626884858446786 24.615922822176152 66-67 22.937001816931303 25.73543475349226 26.750629824259377 24.57693360531706 68-69 23.042758541877088 25.156552763768424 27.378541574585867 24.42214711976862 70-71 22.90237450171355 25.274456614884976 26.931109944624758 24.892058938776717 72-73 22.943460829701717 25.535704404719784 26.677688666511283 24.84314609906721 74-75 23.19087106570701 25.907973653373535 26.097448229341087 24.80370705157837 76-77 23.212943308899046 25.563249382110833 26.308031437424006 24.915775871566115 78-79 23.324636013132352 25.680679127469304 26.18464163057011 24.810043228828235 80-81 23.441051566698068 25.64474273559723 26.00336832887102 24.910837368833686 82-83 23.240470226702698 25.763750164237898 26.022865262994046 24.972914346065362 84-85 22.978564716759877 25.875205162169753 25.986778568131246 25.15945155293912 86-87 23.22577470382922 25.737629652913736 25.95627523533733 25.080320407919714 88-89 23.037898580161087 25.771643760342826 25.63324005435722 25.557217605138867 90-91 22.910784401844698 25.789355374910848 25.781254315195163 25.51860590804929 92-93 22.97690188145271 25.51905404803742 25.98994447332681 25.51409959718306 94-95 22.649956976758403 25.17250753723307 26.434544957821814 25.742990528186716 96-97 23.006646478745683 24.695544801833552 26.351029096821833 25.946779622598932 98-99 22.73362280346527 25.278955537032832 25.916795389191794 26.070626270310104 100-101 22.535960294277654 24.914738154822185 26.26970429950033 26.279597251399828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 644.0 1 463.0 2 345.5 3 461.5 4 458.0 5 446.0 6 721.5 7 1398.5 8 2226.5 9 2671.0 10 2405.5 11 2101.5 12 2112.0 13 2192.0 14 2436.5 15 2637.5 16 2815.0 17 3016.5 18 3262.0 19 3665.0 20 4154.5 21 4770.5 22 5724.0 23 6858.5 24 8156.5 25 9828.5 26 12071.5 27 14785.0 28 17181.0 29 19317.0 30 21914.0 31 26335.5 32 33759.5 33 36120.0 34 34223.0 35 35848.5 36 39566.0 37 44085.5 38 49911.0 39 54359.5 40 58175.0 41 64560.0 42 69632.0 43 75367.5 44 82835.0 45 91335.5 46 99450.5 47 107114.5 48 114691.5 49 117216.0 50 117475.5 51 120515.0 52 126545.5 53 134020.5 54 137341.0 55 132283.5 56 124755.0 57 116731.5 58 108933.0 59 98143.0 60 82826.0 61 68963.5 62 56396.5 63 43142.5 64 31558.5 65 23694.0 66 17582.5 67 13342.5 68 10143.5 69 7511.0 70 5518.5 71 3881.0 72 2838.0 73 2318.0 74 1762.0 75 1239.0 76 829.5 77 539.0 78 354.0 79 202.0 80 140.0 81 104.0 82 59.5 83 41.5 84 25.5 85 10.0 86 4.0 87 3.0 88 2.5 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022528833056660518 2 0.023198337753738096 3 0.0 4 0.0 5 0.0 6 3.3475234853878926E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8453949625797085E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.368808713469731E-5 26-27 3.514899659657287E-4 28-29 8.368808713469731E-5 30-31 4.0170281824654716E-4 32-33 0.0021256774132213116 34-35 0.0041007162696001685 36-37 0.09923733372432407 38-39 0.0917388811170552 40-41 0.10027506600479433 42-43 0.10176471395579192 44-45 0.09592328547379006 46-47 0.08896043662418324 48-49 0.08996469366979962 50-51 0.08879306044991386 52-53 0.07021430510601105 54-55 0.05941854186563509 56-57 0.03242076495598174 58-59 0.014277187665179361 60-61 0.009741293342478767 62-63 0.002008514091232735 64-65 0.005205399019778173 66-67 0.1508561458690054 68-69 0.4046486389136884 70-71 0.38781059578218735 72-73 0.17969506069562208 74-75 0.02900629100088609 76-77 0.030328562777614306 78-79 0.005222136637205113 80-81 0.0015063855684245516 82-83 1.6737617426939463E-5 84-85 4.0170281824654716E-4 86-87 9.540441933355494E-4 88-89 2.510642614040919E-4 90-91 6.527670796506391E-4 92-93 0.002343266439771525 94-95 0.07593857026602435 96-97 0.017189533097466826 98-99 0.01879634437045302 100-101 0.010310372334994709 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2987283.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.97489486620117 #Duplication Level Percentage of deduplicated Percentage of total 1 71.9641728707763 32.365811089962904 2 14.015981780609932 12.607346140590456 3 5.225450666629634 7.050422830805658 4 2.5612374641058353 4.607655427021426 5 1.5172294604565717 3.411861773596872 6 0.9496705032639189 2.562679862509627 7 0.6593667377174668 2.0758464794979177 8 0.4757279551320816 1.711665181358259 9 0.3576379255878016 1.4476255293129887 >10 2.0397357310711817 16.865583841177266 >50 0.1490177748863016 4.610746146407465 >100 0.07708950623945332 6.172561864109834 >500 0.004400153280501377 1.343079474145974 >1k 0.0031323125046330163 2.6444665305868496 >5k 1.4915773831585794E-4 0.5226478289164812 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8495 0.28437212008370144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7087 0.23723898940943997 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4685 0.15683147529042277 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3794 0.12700504103561663 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3644 0.1219837558075348 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3457 0.11572388688985945 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2990 0.10009095221309798 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6737617426939463E-4 2 6.695046970775785E-5 0.0 0.0 0.0 1.6737617426939463E-4 3 6.695046970775785E-5 0.0 0.0 0.0 1.6737617426939463E-4 4 6.695046970775785E-5 0.0 0.0 6.695046970775785E-5 1.6737617426939463E-4 5 6.695046970775785E-5 0.0 0.0 6.695046970775785E-5 1.6737617426939463E-4 6 1.0042570456163678E-4 0.0 0.0 6.695046970775785E-5 2.3432664397715248E-4 7 3.0127711368491036E-4 0.0 0.0 6.695046970775785E-5 2.3432664397715248E-4 8 3.0127711368491036E-4 0.0 0.0 6.695046970775785E-5 2.678018788310314E-4 9 3.0127711368491036E-4 0.0 0.0 1.0042570456163678E-4 2.678018788310314E-4 10-11 3.3475234853878926E-4 0.0 0.0 1.1716332198857624E-4 2.678018788310314E-4 12-13 4.35178053100426E-4 0.0 0.0 1.339009394155157E-4 7.531927842122758E-4 14-15 5.021285228081839E-4 0.0 0.0 1.5063855684245515E-4 8.870937236277915E-4 16-17 5.356037576620628E-4 0.0 0.0 2.0085140912327355E-4 9.707818107624888E-4 18-19 6.52767079650639E-4 0.0 0.0 2.17589026550213E-4 0.0010377322804702466 20-21 7.364551667853364E-4 0.0 0.0 3.012771136849103E-4 0.0011716332198857624 22-23 8.034056364930942E-4 0.0 0.0 6.52767079650639E-4 0.0011716332198857624 24-25 9.707818107624888E-4 0.0 0.0 0.0012385836895935202 0.0011716332198857624 26-27 9.707818107624888E-4 0.0 0.0 0.0018244002995364014 0.0012887965418743386 28-29 0.001305534159301278 0.0 0.0 0.006276606535102299 0.001372484629009036 30-31 0.0014729103335706728 0.0 0.0 0.02465451046988183 0.0013892222464359754 32-33 0.0014896479509976122 0.0 0.0 0.06350252051780833 0.0014394350987167938 34-35 0.0016068112729861884 0.0 0.0 0.11908814799267428 0.0016235488904131278 36-37 0.0018411379169633408 0.0 0.0 0.21447582970880227 0.0016737617426939462 38-39 0.0020085140912327356 0.0 0.0 0.3647461589678648 0.0017574498298286436 40-41 0.0020085140912327356 0.0 0.0 0.530013393441465 0.001774187447255583 42-43 0.002242840735209888 0.0 0.0 0.6928201981533052 0.0017909250646825224 44-45 0.002527380231467859 0.0 0.0 0.8866585455746911 0.0018411379169633408 46-47 0.0028621325800066485 0.0 0.0 1.1214538428397978 0.0019080883866710988 48-49 0.0029792959019952245 0.0 0.0 1.369940511160141 0.0019415636215249776 50-51 0.0029792959019952245 0.0 0.0 1.6388972855936315 0.0020085140912327356 52-53 0.0029792959019952245 0.0 0.0 1.8868818253911666 0.0020754645609404932 54-55 0.0029792959019952245 0.0 0.0 2.1459131926904815 0.0021424150306482512 56-57 0.003146672076264619 0.0 0.0 2.4456839208069674 0.002209365500356009 58-59 0.003146672076264619 0.0 0.0 2.7574722582359956 0.0022763159700637673 60-61 0.003146672076264619 0.0 0.0 3.0746668460939253 0.0023934792920523433 62-63 0.003146672076264619 0.0 0.0 3.3744040989755573 0.0025106426140409197 64-65 0.003146672076264619 1.6737617426939463E-5 0.0 3.6762168164181297 0.002611068318602556 66-67 0.003213622545972377 3.3475234853878926E-5 0.0 4.022384220042092 0.0026445435534564353 68-69 0.003213622545972377 3.3475234853878926E-5 0.0 4.403600194558065 0.0026445435534564353 70-71 0.003247097780826256 3.3475234853878926E-5 0.0 4.834995546120003 0.0026445435534564353 72-73 0.003364261102814832 3.3475234853878926E-5 0.0 5.269972747811305 0.0029123454322874665 74-75 0.0034814244248034085 3.3475234853878926E-5 0.0 5.69684894266797 0.0032638353982531953 76-77 0.0034814244248034085 3.3475234853878926E-5 0.0 6.164581661663792 0.0033475234853878925 78-79 0.0034981620422303477 3.3475234853878926E-5 0.0 6.691029942593319 0.0034144739550956505 80-81 0.0038161767733421977 3.3475234853878926E-5 0.0 7.240710036511439 0.003481424424803408 82-83 0.0038496520081960765 3.3475234853878926E-5 0.0 7.801955824071573 0.003665538216499742 84-85 0.003899864860476895 3.3475234853878926E-5 0.0 8.341007530923585 0.003715751068780561 86-87 0.003950077712757713 3.3475234853878926E-5 0.0 8.879490158783081 0.003866389625623016 88-89 0.003950077712757713 3.3475234853878926E-5 0.0 9.454845757834125 0.004017028182465471 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6015 0.0 40.007687 1 GTATCAA 9755 0.0 35.276203 1 TCAACGC 12270 0.0 27.604351 4 ATCAACG 12320 0.0 27.49232 3 TATCAAC 12475 0.0 27.348644 2 CAACGCA 12560 0.0 26.966988 5 AACGCAG 13015 0.0 26.097128 6 TACATGG 11135 0.0 23.991955 2 GTACATG 11465 0.0 23.515022 1 ACGCAGA 14955 0.0 22.584862 7 CGCAGAG 15180 0.0 22.187605 8 ACATGGG 11410 0.0 22.118204 3 GAGTACA 8180 0.0 20.366898 1 TAAGGTG 1430 0.0 20.235674 5 AGAGTAC 13355 0.0 19.980322 10-11 GAGTACT 8870 0.0 19.948448 12-13 GCAGAGT 16715 0.0 19.781092 9 GTACTTT 9180 0.0 19.093943 14-15 AGGTAAG 1255 0.0 18.904871 2 AGTACTT 9490 0.0 18.795137 12-13 >>END_MODULE