FastQCFastQC Report
Fri 27 May 2016
SRR939879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244908
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98000.18684789132621582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65610.1250927566317655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA158750.034.6691781
GGTATCA102450.030.9822861
TACATGG175600.029.0457922
ATCAACG193300.029.0386943
TATCAAC194800.029.0326442
GTACATG173900.028.9750521
TCAACGC195800.028.6681964
CAACGCA197450.028.188325
AACGCAG204450.027.3160346
ACATGGG190550.025.9218463
ACGCAGA229900.023.9825577
CGCAGAG239250.022.8868668
CATGGGG125500.022.7604644
GCAGAGT251400.020.5350659
AGAGTAC188950.020.06353810-11
GAGTACT114800.019.57014312-13
GAGTACA130800.019.15251
CATGGGA69000.018.8421234
AGTACTT122000.018.78465312-13
TAAGGTG21900.018.6329965
Produced by FastQC (version 0.11.5)