##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939879_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5244908 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.765621627681554 31.0 28.0 33.0 16.0 34.0 2 29.559656527817076 31.0 30.0 33.0 25.0 34.0 3 29.835370992208063 31.0 30.0 33.0 25.0 34.0 4 33.61227327533676 35.0 33.0 37.0 28.0 37.0 5 33.54637793456053 35.0 33.0 37.0 28.0 37.0 6 33.56124854811562 35.0 33.0 37.0 28.0 37.0 7 33.4534037584644 35.0 33.0 37.0 28.0 37.0 8 33.31152920127484 35.0 33.0 37.0 26.0 37.0 9 34.57013850385936 37.0 34.0 39.0 27.0 39.0 10-11 34.384286912182255 37.0 33.0 39.0 27.0 39.0 12-13 34.245408975715115 37.0 33.0 39.0 27.0 39.0 14-15 34.892325852045445 38.0 33.0 40.0 25.5 41.0 16-17 34.69438996832737 38.0 33.0 40.0 25.0 41.0 18-19 34.481981857451075 38.0 33.0 40.0 25.0 41.0 20-21 34.63734807169163 38.0 33.0 40.0 25.0 41.0 22-23 34.52221106642862 38.0 33.0 40.0 24.5 41.0 24-25 34.285472976837724 38.0 32.5 40.0 23.5 41.0 26-27 34.15421271450329 38.0 32.5 40.0 23.5 41.0 28-29 34.21105060374748 38.0 33.0 40.0 24.0 41.0 30-31 33.99831798765584 38.0 32.5 40.0 22.5 41.0 32-33 33.65291402632801 38.0 32.0 40.0 20.0 41.0 34-35 33.457093146343084 37.5 31.0 40.0 19.0 41.0 36-37 33.23684914968956 37.0 31.0 40.0 18.0 41.0 38-39 32.99162616389077 37.0 31.0 40.0 17.0 41.0 40-41 32.73948675553508 37.0 31.0 40.0 15.5 41.0 42-43 32.33225101374514 37.0 30.0 40.0 15.0 41.0 44-45 31.992903784012988 36.5 30.0 39.0 11.0 41.0 46-47 31.61196306970494 36.0 30.0 39.0 9.0 40.5 48-49 31.24475415012046 36.0 29.0 39.0 8.0 40.0 50-51 30.583991368390066 34.5 28.0 38.5 7.5 40.0 52-53 30.71840335426284 35.0 28.5 38.5 6.5 40.0 54-55 30.79073121968965 35.0 28.5 39.0 2.0 40.0 56-57 30.43936852657854 35.0 28.0 39.0 2.0 40.0 58-59 29.99846470138275 35.0 27.5 38.0 2.0 40.0 60-61 29.540253804261198 34.0 27.0 38.0 2.0 40.0 62-63 29.055792589688892 34.0 26.0 38.0 2.0 40.0 64-65 28.47218263885658 34.0 26.0 37.0 2.0 39.5 66-67 27.83817085066125 33.0 25.0 36.5 2.0 39.0 68-69 27.23525446013543 32.5 23.5 36.0 2.0 39.0 70-71 26.622247139511312 32.0 22.0 35.5 2.0 38.5 72-73 25.950018284400794 31.5 20.5 35.0 2.0 37.5 74-75 25.565802679475027 31.0 20.0 35.0 2.0 37.0 76-77 24.978795624251177 31.0 18.0 35.0 2.0 36.5 78-79 24.29117050289538 31.0 12.5 34.0 2.0 36.0 80-81 23.536789682488234 30.0 7.0 34.0 2.0 35.5 82-83 22.76697379629919 29.5 2.0 34.0 2.0 35.0 84-85 21.941381335954794 29.0 2.0 34.0 2.0 35.0 86-87 20.95108274539801 28.0 2.0 33.5 2.0 35.0 88-89 19.893119097608576 26.5 2.0 33.0 2.0 35.0 90-91 18.45172841925921 24.0 2.0 32.5 2.0 34.0 92-93 16.886632043879512 19.0 2.0 31.5 2.0 34.0 94-95 16.335041434473208 17.5 2.0 31.0 2.0 34.0 96-97 15.978121923206277 11.5 2.0 31.0 2.0 34.0 98-99 15.062073920076386 2.0 2.0 31.0 2.0 34.0 100-101 13.48557105291456 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.699860181924283 1101 2 1.0821815504595733 1101 3 1.0401752665669903 1101 4 0.8753285210214727 1101 5 0.9872643033226822 1101 6 1.0513497063886845 1101 7 0.8083347909731131 1101 8 1.0918574780708994 1101 9 1.0758034730841999 1101 10-11 1.1694416928560543 1101 12-13 1.166634945575531 1101 14-15 1.2773519004244633 1101 16-17 1.1335887515855418 1101 18-19 1.1898351646196446 1101 20-21 1.0559317364717344 1101 22-23 1.2780640869148385 1101 24-25 1.4003956714804886 1101 26-27 1.35900292808833 1101 28-29 1.3587931254605152 1101 30-31 1.341472094606111 1101 32-33 1.5701068861743153 1101 34-35 1.5367335190984335 1101 36-37 1.2625022321831736 1101 38-39 1.2775831295749 1101 40-41 1.2697335583768634 1101 42-43 1.4235784125891868 1101 44-45 1.3732354600577779 1101 46-47 1.1806365538656571 1101 48-49 1.079051896741305 1101 50-51 1.12230235919559 1101 52-53 0.8878466919486918 1101 54-55 1.0447231875872944 1101 56-57 1.0221587684830844 1101 58-59 0.9307944166474904 1101 60-61 0.8713593459837128 1101 62-63 0.4302744472781619 1101 64-65 0.5924413751662918 1101 66-67 0.7138066686760354 1101 68-69 0.5527563377626699 1101 70-71 0.6012860700805298 1101 72-73 0.47554726021914107 1101 74-75 0.49248224801350915 1101 76-77 0.3241549649725961 1101 78-79 0.1339339791407852 1101 80-81 0.027823841650388204 1101 82-83 -0.1050926249006352 1101 84-85 -0.0161262669701685 1101 86-87 -0.4074293253420045 1101 88-89 -4.322767562663145 1101 90-91 -7.591844941461773 1101 92-93 -9.901978112643217 1101 94-95 -8.032509605965016 1101 96-97 -4.826268828303295 1101 98-99 -3.366534270710577 1101 100-101 -2.5367028815547332 1102 1 1.9830196895551495 1102 2 1.2630391987460001 1102 3 1.177649048535013 1102 4 1.1787264993776105 1102 5 1.1100760831428715 1102 6 1.141514093364144 1102 7 1.0372931047798062 1102 8 1.3421701529112013 1102 9 1.5480171868805002 1102 10-11 1.4408330400302418 1102 12-13 1.3672227570055142 1102 14-15 1.5264811234318643 1102 16-17 1.6030799871392887 1102 18-19 1.658688699973979 1102 20-21 1.5338760909998683 1102 22-23 1.5420849479877745 1102 24-25 1.7494087734446353 1102 26-27 1.6829663780885298 1102 28-29 1.520396759736741 1102 30-31 1.6623763315614895 1102 32-33 1.8561947227501605 1102 34-35 1.7376944888946682 1102 36-37 1.7531778259723012 1102 38-39 1.654675005241387 1102 40-41 1.5513513444636828 1102 42-43 1.681794712981862 1102 44-45 1.5425546884706378 1102 46-47 1.5935193434124564 1102 48-49 1.2885462115515338 1102 50-51 1.4715818869703625 1102 52-53 1.5939045272303467 1102 54-55 1.6050836008461218 1102 56-57 1.6307551011430554 1102 58-59 1.4705415785008817 1102 60-61 1.4206345144440036 1102 62-63 1.5972803345506854 1102 64-65 1.5577219996252687 1102 66-67 1.5483028609221208 1102 68-69 1.412369037005714 1102 70-71 1.4334518868902038 1102 72-73 1.5143077935501275 1102 74-75 1.4886024842914942 1102 76-77 1.2913939578212776 1102 78-79 1.2723112329600106 1102 80-81 1.3338790655299668 1102 82-83 1.1519065475622696 1102 84-85 1.0973694013731858 1102 86-87 0.9321277536535355 1102 88-89 0.3775110671347086 1102 90-91 -5.062023436288344 1102 92-93 -9.684716812978236 1102 94-95 -7.568273176005986 1102 96-97 -4.03682612505523 1102 98-99 -2.4793185020705426 1102 100-101 -1.607208905860448 1103 1 2.107841388340532 1103 2 1.3904278051059116 1103 3 1.1189244323988063 1103 4 1.015892988034686 1103 5 1.0917184653824137 1103 6 1.020977922889287 1103 7 0.994065354694996 1103 8 1.0118104895569573 1103 9 1.1463070583449806 1103 10-11 1.100211403956493 1103 12-13 0.995292314193243 1103 14-15 1.1654896963549035 1103 16-17 1.1418653715023481 1103 18-19 1.097653750369588 1103 20-21 0.9672888987313115 1103 22-23 1.0840097635507107 1103 24-25 1.0129353440001267 1103 26-27 1.050766146932709 1103 28-29 0.9707454415559198 1103 30-31 1.1232703899607657 1103 32-33 1.1045778217780793 1103 34-35 1.043708308317818 1103 36-37 1.1157566996172221 1103 38-39 1.0831551043295065 1103 40-41 0.9662079942532671 1103 42-43 0.9877432077859893 1103 44-45 0.8777025297486105 1103 46-47 1.0581817574464232 1103 48-49 1.073162368756158 1103 50-51 1.0570298715425537 1103 52-53 1.0097712006161181 1103 54-55 0.9069800445271987 1103 56-57 0.9343385531105604 1103 58-59 0.9299703823651093 1103 60-61 0.9520175112855576 1103 62-63 0.8664503106092099 1103 64-65 0.8529453584958624 1103 66-67 0.9095436297368948 1103 68-69 0.7452637038571517 1103 70-71 0.6907825923074356 1103 72-73 0.880537963445466 1103 74-75 0.6841299175985576 1103 76-77 0.4548671838262628 1103 78-79 0.23751578300636922 1103 80-81 0.12970440243002557 1103 82-83 -0.16825011020624814 1103 84-85 -0.09079775546142699 1103 86-87 -0.2312535512803855 1103 88-89 -1.0237986564814925 1103 90-91 -6.3869677470506225 1103 92-93 -9.568711663065233 1103 94-95 -8.399325921903017 1103 96-97 -5.196254823933465 1103 98-99 -3.5771263711674735 1103 100-101 -2.731162720421585 1104 1 2.057308368237578 1104 2 1.3275090936742018 1104 3 1.2109953079786067 1104 4 0.947916310566697 1104 5 0.9614063799903008 1104 6 0.9977552771014402 1104 7 0.964119705610706 1104 8 1.1027934260277164 1104 9 1.1899285123841068 1104 10-11 1.2155460608323096 1104 12-13 1.0581472705916113 1104 14-15 1.0922512769512736 1104 16-17 1.1255157585164213 1104 18-19 1.1969189360778927 1104 20-21 1.1955047946927095 1104 22-23 1.1601555926303746 1104 24-25 1.281187799519735 1104 26-27 1.1440810963970804 1104 28-29 1.0740539174322947 1104 30-31 1.2192583467650806 1104 32-33 1.2652300968218952 1104 34-35 1.2930716587619244 1104 36-37 1.2583136547809204 1104 38-39 1.2013888637105978 1104 40-41 1.1382936385814517 1104 42-43 1.1367889640828182 1104 44-45 1.0705313796118077 1104 46-47 1.0984218927311993 1104 48-49 1.0923780557371643 1104 50-51 1.1721670579618717 1104 52-53 1.0762137538280498 1104 54-55 1.0994595928733304 1104 56-57 1.0777507465159104 1104 58-59 0.9640947385093348 1104 60-61 1.0596536227769988 1104 62-63 0.977083609272615 1104 64-65 0.904607853561604 1104 66-67 0.9515043502308238 1104 68-69 0.757935542188001 1104 70-71 0.6757880490176653 1104 72-73 0.6464201729687282 1104 74-75 0.6439764571314832 1104 76-77 0.8077003494147981 1104 78-79 0.5402708211552998 1104 80-81 0.34944007826540613 1104 82-83 0.11541414878211143 1104 84-85 0.04956550610595656 1104 86-87 0.16593049799094572 1104 88-89 -0.2831145980022143 1104 90-91 -3.027470529052046 1104 92-93 -7.491822792592242 1104 94-95 -5.374044799237932 1104 96-97 -2.920111456979111 1104 98-99 -2.019320673289064 1104 100-101 -1.4926908715216154 1105 1 1.9458351697852443 1105 2 1.242056503493739 1105 3 1.0173422507631287 1105 4 0.9955493897326875 1105 5 0.9133784061619608 1105 6 0.9044683522715502 1105 7 0.9436722745379313 1105 8 0.8536047032538292 1105 9 1.037525253195767 1105 10-11 0.9979344293407664 1105 12-13 0.9213192871088012 1105 14-15 1.0950236855472326 1105 16-17 0.9578328247373733 1105 18-19 1.0582607599816924 1105 20-21 0.8728414279777041 1105 22-23 0.8799513469428746 1105 24-25 1.0827354647804697 1105 26-27 0.8477754734054983 1105 28-29 0.9598309045268181 1105 30-31 1.101349404512895 1105 32-33 1.2095786446621446 1105 34-35 1.1973750049075491 1105 36-37 1.1162459756016574 1105 38-39 1.0642739614319439 1105 40-41 0.9023213495936488 1105 42-43 0.9468104720977024 1105 44-45 1.0150864928942838 1105 46-47 0.9867471236842285 1105 48-49 0.8695298796610729 1105 50-51 0.9264774103031144 1105 52-53 0.7717019314595781 1105 54-55 0.7989805232961089 1105 56-57 0.7246481751415494 1105 58-59 0.7540208013559138 1105 60-61 0.6145248730376345 1105 62-63 0.7318211048895513 1105 64-65 0.7957446808746376 1105 66-67 0.6791448982024804 1105 68-69 0.7446862228616951 1105 70-71 0.6458152424429748 1105 72-73 0.7536387334201251 1105 74-75 0.6040871684313025 1105 76-77 0.5920808575751728 1105 78-79 0.44753480454960837 1105 80-81 0.44944423505132747 1105 82-83 -0.02010622382681504 1105 84-85 -0.03226189743816832 1105 86-87 0.1950820711668051 1105 88-89 -0.13924116408806952 1105 90-91 -3.2462571900601205 1105 92-93 -7.738703359416933 1105 94-95 -5.615000605228666 1105 96-97 -3.1193080910552347 1105 98-99 -2.014325423422653 1105 100-101 -1.406050814989328 1106 1 1.7655010391711734 1106 2 1.1194048794973703 1106 3 0.8498017314740132 1106 4 0.8075180693616844 1106 5 0.938362385073134 1106 6 0.8322165350615407 1106 7 0.9008662580187092 1106 8 -0.09531416485452837 1106 9 -0.32882613765430335 1106 10-11 -0.4576471190305682 1106 12-13 -0.22742708842002912 1106 14-15 0.6938175068273651 1106 16-17 0.827137687875414 1106 18-19 0.9920945893855659 1106 20-21 0.8782868485535857 1106 22-23 0.8997049721001673 1106 24-25 0.930128075456345 1106 26-27 1.022386713279964 1106 28-29 0.7624100596836882 1106 30-31 0.845920142251444 1106 32-33 0.8403554307562544 1106 34-35 0.9623178092773585 1106 36-37 0.9596195228971496 1106 38-39 0.8415565720666578 1106 40-41 0.901483793142468 1106 42-43 0.9538044492661086 1106 44-45 0.7846886209888879 1106 46-47 0.8308664578107319 1106 48-49 0.6463357685604869 1106 50-51 0.6936663919107673 1106 52-53 0.761628405982961 1106 54-55 0.8349659128864495 1106 56-57 0.8102708431640053 1106 58-59 0.8106055031715478 1106 60-61 0.5556231855744258 1106 62-63 0.7056334516257117 1106 64-65 0.7590552896485683 1106 66-67 0.7616227328373952 1106 68-69 0.7985019522088983 1106 70-71 0.7370312259319896 1106 72-73 0.6499458209851561 1106 74-75 0.4564694391582016 1106 76-77 0.43100109107825446 1106 78-79 0.45442757783782284 1106 80-81 0.3165729403140638 1106 82-83 0.036100044089700134 1106 84-85 -0.0025133084604078704 1106 86-87 0.1528038586125824 1106 88-89 -0.08875147453354515 1106 90-91 -3.497578809816215 1106 92-93 -8.227607811975984 1106 94-95 -5.820449799508554 1106 96-97 -3.0723003981880836 1106 98-99 -2.0534972943702083 1106 100-101 -1.5225005965947833 1107 1 1.8291543073923435 1107 2 1.1253806752060527 1107 3 0.9013688321901192 1107 4 1.0257987874138195 1107 5 0.7034663543451387 1107 6 0.726495738479656 1107 7 0.6932690562243309 1107 8 0.5707103224977601 1107 9 0.6269802217731524 1107 10-11 0.695181702899788 1107 12-13 0.7327562335895053 1107 14-15 0.778039357831716 1107 16-17 0.8191994457817557 1107 18-19 0.8575595665608944 1107 20-21 0.7103089385721262 1107 22-23 0.6967051025036142 1107 24-25 0.5902932108662853 1107 26-27 0.6959532455673028 1107 28-29 0.6914522985955571 1107 30-31 0.9082396586541179 1107 32-33 0.9941149207959938 1107 34-35 0.8842676983937139 1107 36-37 0.6809807171104936 1107 38-39 0.689289156658937 1107 40-41 0.6014521217949849 1107 42-43 0.7796713709861862 1107 44-45 0.5404757998466536 1107 46-47 0.5311380999999535 1107 48-49 0.6277987860202998 1107 50-51 0.6581371683079311 1107 52-53 0.5776776170692344 1107 54-55 0.6064177042512462 1107 56-57 0.4419723218522691 1107 58-59 0.45522981436384313 1107 60-61 0.09734835399638797 1107 62-63 0.25519263097686107 1107 64-65 0.4838449852521798 1107 66-67 0.6135317897979249 1107 68-69 0.5342127500622489 1107 70-71 0.4275630128242547 1107 72-73 0.3767759540061668 1107 74-75 0.23414253345292124 1107 76-77 0.31015022078788945 1107 78-79 0.2354833762658437 1107 80-81 0.24605398479548413 1107 82-83 0.09764105800975997 1107 84-85 0.07876226930761021 1107 86-87 0.21824133707938742 1107 88-89 0.20846122299002445 1107 90-91 -2.9883944582843434 1107 92-93 -4.346256003825648 1107 94-95 -2.658169746771824 1107 96-97 -1.3210447222117505 1107 98-99 -0.7400649385470981 1107 100-101 -0.32746025633541365 1108 1 1.7033657541478995 1108 2 0.9766339316682533 1108 3 -0.42281769491569676 1108 4 -0.2009064488914447 1108 5 0.3750378589811021 1108 6 0.6021603584318882 1108 7 0.602990930876139 1108 8 0.5321829383733174 1108 9 0.8110494213617869 1108 10-11 0.6879280656283555 1108 12-13 0.6226359851293282 1108 14-15 0.641307064284895 1108 16-17 0.5897535669955332 1108 18-19 0.5504665243898401 1108 20-21 0.5851388921695246 1108 22-23 0.4699100484175176 1108 24-25 0.3403900431271225 1108 26-27 0.5415021322760722 1108 28-29 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3.3981371553714617 2311 94-95 3.097835934250785 2311 96-97 2.287245330206254 2311 98-99 1.9664848248673419 2311 100-101 1.9287986769654193 2312 1 1.2044715471474206 2312 2 0.5272758831489419 2312 3 -0.15534661889404688 2312 4 -0.7088555504616139 2312 5 -0.12693430405104067 2312 6 -0.38983505604618784 2312 7 0.007910220077057772 2312 8 0.05849420550156026 2312 9 0.047526557439958594 2312 10-11 0.08329515565701229 2312 12-13 0.1412156607317172 2312 14-15 0.22232183769664005 2312 16-17 -0.06501470654681896 2312 18-19 -0.4298728344574769 2312 20-21 0.2538891215535628 2312 22-23 0.36825362577229726 2312 24-25 0.39866211742811686 2312 26-27 0.3441412901289951 2312 28-29 0.37988658226156957 2312 30-31 0.2024489209145699 2312 32-33 0.40219475933797355 2312 34-35 0.29729703937428553 2312 36-37 0.372168689817336 2312 38-39 0.31588110509219547 2312 40-41 0.3187540875362984 2312 42-43 0.35586077030703933 2312 44-45 0.23198702403648497 2312 46-47 0.3508926156573544 2312 48-49 0.41703861263603414 2312 50-51 0.23827397776369708 2312 52-53 0.26547699238320277 2312 54-55 0.2882614028835313 2312 56-57 0.30697653371489864 2312 58-59 0.21331267385865615 2312 60-61 0.3518904089952599 2312 62-63 0.4090186439156902 2312 64-65 0.35664112709833873 2312 66-67 0.5039753946440761 2312 68-69 0.4327218383653708 2312 70-71 0.584488170766722 2312 72-73 0.5551515924326118 2312 74-75 0.5695604195394282 2312 76-77 0.84120270931923 2312 78-79 0.8546147624283051 2312 80-81 0.9956036214012229 2312 82-83 1.0000556887734042 2312 84-85 1.3338772570646533 2312 86-87 1.714361016874907 2312 88-89 2.0900283884919233 2312 90-91 2.672926186030576 2312 92-93 3.666190575976078 2312 94-95 3.467197778665014 2312 96-97 2.619147034913553 2312 98-99 2.235816501474728 2312 100-101 2.1924688678544637 2313 1 -7.189856716249558 2313 2 -2.6699786803930614 2313 3 -1.6381730784484212 2313 4 -0.8181028506905434 2313 5 -0.6663028992112316 2313 6 -0.2984298566646473 2313 7 -0.17407742270217597 2313 8 -0.14930912171908517 2313 9 -0.203842265447868 2313 10-11 -0.18565789649261433 2313 12-13 -0.46872198384537 2313 14-15 -0.34449381078776753 2313 16-17 -0.590785474708099 2313 18-19 -0.7706867510132724 2313 20-21 -0.5709953366344465 2313 22-23 -1.2203204995737948 2313 24-25 -0.4129545090865747 2313 26-27 -0.5036280577309711 2313 28-29 -0.683536104205416 2313 30-31 -0.2265062586906268 2313 32-33 -0.5902087860217762 2313 34-35 -0.42046679507971163 2313 36-37 -0.2586498112553741 2313 38-39 -0.25873148824617687 2313 40-41 -0.3067619253123368 2313 42-43 -0.16893861962260814 2313 44-45 -0.37927356829508696 2313 46-47 -1.3431183871832495 2313 48-49 -0.8488850926124911 2313 50-51 -0.9511522984099976 2313 52-53 -1.301372515605241 2313 54-55 -1.0622641385688922 2313 56-57 -0.7929977869975282 2313 58-59 -0.599008709766526 2313 60-61 -0.4938975885596619 2313 62-63 -0.44504692366548326 2313 64-65 -0.47905820649868147 2313 66-67 -0.3371115553637125 2313 68-69 -0.40814346672973656 2313 70-71 -0.4045920492900912 2313 72-73 -0.39543657412018973 2313 74-75 -0.3219152855173242 2313 76-77 -0.10298861785347313 2313 78-79 -0.1394395086634681 2313 80-81 -0.03651886011088834 2313 82-83 0.22133300110785115 2313 84-85 0.27480467786936913 2313 86-87 0.8206154066247642 2313 88-89 1.1677729859533628 2313 90-91 1.9220119794216792 2313 92-93 2.6670936958447164 2313 94-95 2.4867431324219176 2313 96-97 1.7145304263773617 2313 98-99 1.323124700868254 2313 100-101 1.1525483627785338 2314 1 0.3494761042694279 2314 2 -0.08430206592929324 2314 3 -1.5873321029793743 2314 4 -0.4478187194901935 2314 5 -0.28289909396850277 2314 6 -3.827604626198667 2314 7 -3.015994286682176 2314 8 -2.8363557317357895 2314 9 -3.5407596702867536 2314 10-11 -2.7291571332806406 2314 12-13 -1.3887894409672796 2314 14-15 -1.4150745730382255 2314 16-17 -2.431558392100918 2314 18-19 -3.5905312540841514 2314 20-21 -3.2291064771255478 2314 22-23 -4.436898605606174 2314 24-25 -3.436684151759138 2314 26-27 -2.178185094305043 2314 28-29 -2.052893263374173 2314 30-31 -2.1276348809427645 2314 32-33 -2.4505033137317724 2314 34-35 -1.803455821653909 2314 36-37 -2.289935372726994 2314 38-39 -3.6369806378958174 2314 40-41 -2.9275751667397216 2314 42-43 -2.0529455726640364 2314 44-45 -2.5301631609682538 2314 46-47 -2.0597745571812105 2314 48-49 -2.630407400036681 2314 50-51 -3.380356364466895 2314 52-53 -2.2237306002436483 2314 54-55 -2.775623262858261 2314 56-57 -1.842645297243802 2314 58-59 -1.137770022731516 2314 60-61 -1.59315889551511 2314 62-63 -1.2984832705843914 2314 64-65 -1.9378111962464004 2314 66-67 -1.2687075998824113 2314 68-69 -1.2649750552923749 2314 70-71 -1.1645773860047903 2314 72-73 -2.263893894344708 2314 74-75 -2.4144139033224015 2314 76-77 -1.4521658513001867 2314 78-79 -2.2615208538396097 2314 80-81 -1.2705512034721487 2314 82-83 -0.8876742223630174 2314 84-85 -1.8877992271317723 2314 86-87 -0.5661844215022995 2314 88-89 0.3765102006503014 2314 90-91 1.561528631865766 2314 92-93 2.618185629835896 2314 94-95 2.367080297840605 2314 96-97 1.591146903342782 2314 98-99 1.4428186348353922 2314 100-101 1.268285403198723 2315 1 -3.1059718939548198 2315 2 -2.20219064163933 2315 3 -2.8789166562393547 2315 4 -4.26173947775516 2315 5 -4.066869338680572 2315 6 -3.415799869137402 2315 7 -6.07262888226651 2315 8 -3.3819253136231318 2315 9 -2.774863204558148 2315 10-11 -4.170522271028041 2315 12-13 -3.126314899572378 2315 14-15 -2.122519335840373 2315 16-17 -2.6489540899802932 2315 18-19 -2.1933483928814184 2315 20-21 -1.538539274749425 2315 22-23 -0.9471211118726899 2315 24-25 -2.4935183028199575 2315 26-27 -4.203795117058448 2315 28-29 -3.591311481222789 2315 30-31 -3.602604963539882 2315 32-33 -4.153875209112748 2315 34-35 -3.961307005226736 2315 36-37 -3.074749366519761 2315 38-39 -2.019086363329638 2315 40-41 -1.6105758481828332 2315 42-43 -3.1814134221373145 2315 44-45 -6.6098216765938815 2315 46-47 -3.3614556051288993 2315 48-49 -2.3788220674024068 2315 50-51 -3.6111991342357825 2315 52-53 -3.681185487270671 2315 54-55 -2.058601715578707 2315 56-57 -1.5728693113194012 2315 58-59 -2.9014514799780606 2315 60-61 -3.2275534917005295 2315 62-63 -4.983209113578933 2315 64-65 -4.460344632613353 2315 66-67 -3.9106597707518205 2315 68-69 -2.3185996042072183 2315 70-71 -3.6303762580260575 2315 72-73 -2.627430167659554 2315 74-75 -1.7466674997463443 2315 76-77 -3.4522488826790436 2315 78-79 -2.024131658386924 2315 80-81 -1.6888934081309976 2315 82-83 -1.3365170234596953 2315 84-85 -2.6155005581680726 2315 86-87 -2.102814758544749 2315 88-89 -1.4680970832137348 2315 90-91 -0.7111595650581783 2315 92-93 -0.16933493857756687 2315 94-95 -0.09807383934429836 2315 96-97 -0.29899019776434876 2315 98-99 -0.46858261774134924 2315 100-101 -0.8907669018580542 2316 1 -7.501126557519399 2316 2 -6.302658848245642 2316 3 -3.7613951182221825 2316 4 -4.444118176361041 2316 5 -2.293564347851987 2316 6 -3.0992818909649564 2316 7 -3.5424089315509235 2316 8 -2.81700935890208 2316 9 -7.30173772248126 2316 10-11 -7.147039031322031 2316 12-13 -6.382858546516417 2316 14-15 -5.1990075856463775 2316 16-17 -5.121153564472742 2316 18-19 -4.4648999420368085 2316 20-21 -3.3397701952368912 2316 22-23 -3.86983062546323 2316 24-25 -3.7022228907687484 2316 26-27 -4.414806674771654 2316 28-29 -4.608583540804574 2316 30-31 -7.261992939953167 2316 32-33 -5.3576444729402155 2316 34-35 -6.051951925530357 2316 36-37 -4.798686300947036 2316 38-39 -7.12902112517374 2316 40-41 -4.480613868388417 2316 42-43 -5.159407968281613 2316 44-45 -3.784695024233784 2316 46-47 -4.8860959460228806 2316 48-49 -6.002221618793847 2316 50-51 -5.362447226342859 2316 52-53 -7.069994117502706 2316 54-55 -5.988617450008409 2316 56-57 -5.127110631368993 2316 58-59 -3.807206118998419 2316 60-61 -3.8478640180433317 2316 62-63 -4.815482519359701 2316 64-65 -3.7055328461629777 2316 66-67 -5.017329125150248 2316 68-69 -3.9161885314989426 2316 70-71 -3.3471189604376903 2316 72-73 -2.8057481048627544 2316 74-75 -2.8967059259114123 2316 76-77 -3.5207946751422874 2316 78-79 -5.411650783978192 2316 80-81 -6.3101413742506445 2316 82-83 -3.935766068405009 2316 84-85 -2.7629908886330945 2316 86-87 -2.9035193315616965 2316 88-89 -1.8859133851468037 2316 90-91 -1.6596017771920764 2316 92-93 -1.7164950653302533 2316 94-95 -2.4903080443706376 2316 96-97 -3.9051069573117463 2316 98-99 -4.943741746860264 2316 100-101 -4.41889161167868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43993.0 3 15877.0 4 15938.0 5 18260.0 6 20634.0 7 24266.0 8 28828.0 9 34105.0 10 39765.0 11 46108.0 12 51721.0 13 56338.0 14 61459.0 15 66317.0 16 72614.0 17 77924.0 18 77355.0 19 79675.0 20 86769.0 21 95542.0 22 106165.0 23 119121.0 24 133311.0 25 148407.0 26 168116.0 27 188951.0 28 212807.0 29 239200.0 30 267184.0 31 297466.0 32 326278.0 33 354937.0 34 397828.0 35 447102.0 36 446847.0 37 306109.0 38 70776.0 39 815.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.43558756568679 18.317539865500173 12.311611945570048 23.935260623242982 2 16.48879319839905 19.518181118834264 38.301191776734804 25.69183390603188 3 19.997445743038693 23.118526304455976 28.772711983283173 28.111315969222154 4 13.51472250934655 15.013015100724317 35.40588518531388 36.06637720461525 5 14.688108607332707 36.53696151912208 33.379657963872134 15.395271909673081 6 34.39857813770253 34.200588127701835 16.665202902529728 14.735630832065905 7 30.143597141922424 31.63014048414213 19.914814575026828 18.311447798908617 8 27.54319796408583 33.690029508686955 19.42314364808948 19.343628879137743 9 27.35906494129528 15.73030167262327 19.168955537654554 37.741677848426896 10-11 26.183615489602925 25.10371871084589 26.68259200434487 22.03007379520632 12-13 26.80026216912009 22.37323201728445 27.12123316868998 23.705272644905484 14-15 23.71382234480778 23.83923299666437 24.851760503941723 27.59518415458612 16-17 23.65881739328687 27.1013075736197 25.515839831325422 23.72403520176801 18-19 23.8163152591651 25.736019262544602 27.34030826488138 23.107357213408918 20-21 24.223645189235768 25.081617522356726 27.349872269102722 23.344865019304784 22-23 24.07513033723042 24.926185142916967 27.304299282575663 23.69438523727695 24-25 23.834687746153367 25.15180532533999 27.41429515434497 23.59921177416168 26-27 23.4315081491799 25.415447731399578 27.38489100630747 23.76815311311305 28-29 23.530801233297268 25.834867852065862 27.170780635661984 23.463550278974886 30-31 23.516612576659888 25.496548555315744 26.984710874446566 24.0021279935778 32-33 23.357653214294587 25.489572962509293 27.075451507061878 24.077322316134246 34-35 24.05984314947613 25.5202770798544 26.920331707837164 23.499548062832307 36-37 24.095581929333495 25.226546412634548 27.024891728566995 23.652979929464966 38-39 23.678995175676214 25.422435231215314 27.01574508952928 23.882824503579194 40-41 23.88396207352661 25.353233155904288 27.13834163156062 23.624463139008483 42-43 23.6178055410535 25.319623438017206 26.99436800149792 24.068203019431376 44-45 23.487610772390827 25.388925469002643 27.250340086798193 23.873123671808337 46-47 23.82342049841044 25.722468137111594 26.74621545201526 23.707895912462707 48-49 23.582125442496675 25.814911164244002 26.573474160029274 24.02948923323005 50-51 23.47060439888654 26.034230553265715 26.722626429160247 23.772538618687502 52-53 23.841246580938357 25.64100775079698 26.560821113853773 23.956924554410893 54-55 23.26457272239835 25.81791611670603 26.793919832703644 24.123591328191978 56-57 23.216630989365033 25.977799013546417 26.68757395081453 24.117996046274015 58-59 23.32833946793022 25.53214096555475 26.65757139142901 24.481948175086025 60-61 22.994449303421394 25.786036637577762 27.01915328473847 24.200360774262375 62-63 23.109480923142492 25.682385440329845 26.97501760604916 24.2331160304785 64-65 22.891543939253847 25.815271587174355 26.755165020070166 24.53801945350164 66-67 22.893394818234324 25.715027533133277 26.771924247249697 24.619653401382706 68-69 23.229463461213125 25.41732296401827 26.871759728349492 24.481453846419114 70-71 23.233362100704653 25.434362150571495 26.500117788915766 24.83215795980809 72-73 23.20167398546357 25.642749890177434 26.445751856710913 24.709824267648084 74-75 23.052043614957523 25.980237569641545 26.372179696683006 24.595539118717927 76-77 23.33339354698859 25.545366067005563 26.416017291067934 24.705223094937914 78-79 23.39035448471942 25.789499740896936 26.303854203443024 24.51629157094062 80-81 23.556674813531615 25.590437417729618 26.25739447716541 24.595493291573362 82-83 23.450284488052937 25.62142657436238 26.342977171655864 24.58531176592882 84-85 23.239903260282897 25.75542068092406 26.28704380426988 24.71763225452316 86-87 23.26642595607156 25.54720376054399 26.534186007707554 24.6521842756769 88-89 23.415750992765844 25.57202541052416 26.09455588159289 24.917667715117105 90-91 23.116028275792672 25.477166687523116 26.487372049989222 24.91943298669499 92-93 23.437779708862596 25.14838143163188 26.65059091291316 24.76324794659237 94-95 23.331398889705245 24.98960957940821 26.659097880966385 25.01989364992016 96-97 23.852702739134866 24.70035902423877 26.15649418576526 25.2904440508611 98-99 23.85486289683775 24.912226644560395 25.83518930957684 25.39772114902502 100-101 24.156183403540588 24.712666766142885 25.874386403896406 25.25676342642012 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6431.0 1 3711.5 2 958.5 3 922.0 4 840.0 5 812.5 6 1033.0 7 1573.5 8 2312.0 9 2661.5 10 2495.0 11 2373.5 12 2394.0 13 2547.0 14 2811.0 15 3089.5 16 3430.0 17 3799.5 18 4247.0 19 4806.0 20 5540.0 21 6620.5 22 7918.0 23 9628.0 24 11779.5 25 14455.0 26 18275.0 27 23007.0 28 27762.5 29 32867.5 30 38808.0 31 48019.5 32 60071.5 33 65512.0 34 66052.5 35 69945.5 36 76939.5 37 85902.0 38 96016.0 39 104641.0 40 113551.5 41 124533.0 42 135569.5 43 147912.0 44 160236.5 45 174017.0 46 187954.5 47 202074.5 48 215416.5 49 219586.5 50 219265.0 51 221195.0 52 224459.5 53 228394.5 54 227582.0 55 217981.5 56 204518.5 57 189202.0 58 174096.0 59 155299.0 60 130298.5 61 106177.0 62 85594.5 63 66175.5 64 49244.0 65 36946.5 66 26891.5 67 19688.0 68 14895.0 69 11333.0 70 8687.5 71 6610.5 72 5022.0 73 3813.0 74 2983.5 75 2317.5 76 1690.5 77 1204.0 78 857.0 79 593.0 80 379.5 81 246.0 82 171.0 83 125.0 84 86.5 85 58.0 86 43.5 87 31.0 88 21.5 89 13.0 90 17.5 91 16.0 92 8.5 93 5.5 94 4.0 95 5.0 96 5.0 97 4.5 98 3.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1331386556256087 2 0.11933479100110049 3 0.1257219383066395 4 0.136246431777259 5 0.12419664939785408 6 0.13861062958587644 7 0.1678008460777577 8 0.15529347702571714 9 0.16591330105313573 10-11 0.15994560819751272 12-13 0.1492971850030544 14-15 0.15466429535084314 16-17 0.14950691222801238 18-19 0.16457867325794848 20-21 0.16968839110237968 22-23 0.2039597262716524 24-25 0.22669606406823534 26-27 0.23910810256347678 28-29 0.23391258721792643 30-31 0.22516124210377 32-33 0.22646727073191752 34-35 0.21595231031697792 36-37 0.22429373403689826 38-39 0.21196749304277598 40-41 0.21660055810321174 42-43 0.20926010522968183 44-45 0.2017671234652734 46-47 0.21716300838832636 48-49 0.2549425080478056 50-51 0.25683005307242757 52-53 0.25882246170952855 54-55 0.2568777183508271 56-57 0.231128934959393 58-59 0.23173905052290716 60-61 0.20229144152766834 62-63 0.19450293503718272 64-65 0.19584709588804988 66-67 0.20341634209789763 68-69 0.20367373460125518 70-71 0.209136175505843 72-73 0.24031880063482525 74-75 0.2432454487285573 76-77 0.25808841642217556 78-79 0.2554668261102006 80-81 0.26809812488608 82-83 0.26731641432032743 84-85 0.2425972009423235 86-87 0.2459337704302916 88-89 0.2490034143592223 90-91 0.43284839314626683 92-93 0.5762255505720977 94-95 0.33818515024477075 96-97 0.2840659931499275 98-99 0.315115155499391 100-101 0.33419079991488887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5244908.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.280298305497254 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46430167288123 28.383230912722052 2 12.162400135853463 9.798102111659956 3 5.484149028121273 6.627094764135831 4 3.2051672651367094 5.1642037423488585 5 1.9783516890811619 3.984429809468678 6 1.3546838778063757 3.2740242424613153 7 0.9805400519181776 2.7647512054226366 8 0.6871000697370587 2.214127661978935 9 0.5438257596642834 1.9714917442941886 >10 2.8678639635272285 20.572429059067456 >50 0.17219065014198878 4.734466571070229 >100 0.09138737297821552 6.625501677916559 >500 0.004871797930876486 1.322326303668882 >1k 0.0030692293690382936 2.2427564107438713 >5k 9.7435852985171E-5 0.32106378304058836 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9800 0.18684789132621582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6561 0.1250927566317655 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.719833407945382E-5 2 0.0 5.719833407945382E-5 0.0 0.0 5.719833407945382E-5 3 0.0 5.719833407945382E-5 0.0 0.0 5.719833407945382E-5 4 0.0 5.719833407945382E-5 0.0 0.0 5.719833407945382E-5 5 0.0 1.1439666815890764E-4 0.0 0.0 5.719833407945382E-5 6 3.8132222719635886E-5 1.1439666815890764E-4 0.0 0.0 7.626444543927177E-5 7 3.8132222719635886E-5 1.1439666815890764E-4 0.0 0.0 7.626444543927177E-5 8 3.8132222719635886E-5 1.1439666815890764E-4 0.0 0.0 7.626444543927177E-5 9 3.8132222719635886E-5 1.1439666815890764E-4 0.0 0.0 9.53305567990897E-5 10-11 3.8132222719635886E-5 1.334627795187256E-4 0.0 0.0 9.53305567990897E-5 12-13 3.8132222719635886E-5 1.6206194655845252E-4 0.0 0.0 1.1439666815890764E-4 14-15 3.8132222719635886E-5 3.0505778175708703E-4 0.0 2.8599167039726914E-5 2.0019416927808837E-4 16-17 3.8132222719635886E-5 4.0038833855617675E-4 0.0 5.719833407945382E-5 2.0972722495799735E-4 18-19 3.8132222719635886E-5 4.2898750559590366E-4 0.0 5.719833407945382E-5 2.4785944767763326E-4 20-21 3.8132222719635886E-5 4.3852056127581263E-4 0.0 1.0486361247899869E-4 2.669255590374512E-4 22-23 3.8132222719635886E-5 4.3852056127581263E-4 0.0 3.3365694879681395E-4 3.1459083743699606E-4 24-25 3.8132222719635886E-5 4.6711972831553955E-4 0.0 8.293758441520804E-4 3.5272306015663194E-4 26-27 3.8132222719635886E-5 4.766527839954485E-4 0.0 0.0019447433587014302 3.6225611583654086E-4 28-29 3.8132222719635886E-5 4.766527839954485E-4 0.0 0.009399592900390245 3.6225611583654086E-4 30-31 3.8132222719635886E-5 4.766527839954485E-4 0.0 0.03732191298684362 3.6225611583654086E-4 32-33 3.8132222719635886E-5 5.052519510351755E-4 0.0 0.09892451879041539 3.6225611583654086E-4 34-35 3.8132222719635886E-5 5.624502851146293E-4 0.0 0.186514234377419 3.813222271963588E-4 36-37 3.8132222719635886E-5 6.959130646333549E-4 0.0 0.3309400279280399 3.813222271963588E-4 38-39 3.8132222719635886E-5 8.675080668717163E-4 0.0 0.5605532070343274 4.0038833855617675E-4 40-41 3.8132222719635886E-5 0.001000970846390442 0.0 0.8195377306904144 4.0038833855617675E-4 42-43 3.8132222719635886E-5 0.0012011650156685304 0.0 1.0794564938031326 4.194544499159947E-4 44-45 3.8132222719635886E-5 0.0012202311270283484 0.0 1.382998519707114 4.480536169557216E-4 46-47 3.8132222719635886E-5 0.0012583633497479842 0.0 1.732346878153058 4.766527839954486E-4 48-49 3.8132222719635886E-5 0.001306028628147529 0.0 2.1015525915802526 4.957188953552665E-4 50-51 3.8132222719635886E-5 0.001363226962226983 0.0 2.5146961586361476 4.957188953552665E-4 52-53 3.8132222719635886E-5 0.0014299583519863455 0.0 2.9054275117885764 5.147850067150845E-4 54-55 3.8132222719635886E-5 0.0014871566860657993 0.0 3.318838004403509 5.338511180749024E-4 56-57 3.8132222719635886E-5 0.0014871566860657993 0.0 3.7827450929549196 5.338511180749024E-4 58-59 3.8132222719635886E-5 0.0015062227974256173 0.0 4.275289480768777 5.338511180749024E-4 60-61 3.8132222719635886E-5 0.0016396855769443429 0.0 4.7993978159388115 5.338511180749024E-4 62-63 5.719833407945382E-5 0.0016778177996639788 0.0 5.3073380886757215 5.529172294347203E-4 64-65 5.719833407945382E-5 0.0016778177996639788 0.0 5.821827570664729 5.529172294347203E-4 66-67 5.719833407945382E-5 0.0017922144678228864 0.0 6.392142626715283 5.529172294347203E-4 68-69 5.719833407945382E-5 0.0018017475235027954 0.0 7.007787362523803 5.529172294347203E-4 70-71 5.719833407945382E-5 0.0018970780803018853 0.0 7.647207539198018 5.719833407945383E-4 72-73 5.719833407945382E-5 0.001982875581421066 0.0 8.292709805396015 6.57780841913719E-4 74-75 5.719833407945382E-5 0.002001941692780884 0.0 8.92267319083576 6.67313897593628E-4 76-77 6.67313897593628E-5 0.00204007391550052 0.0 9.581397805261789 6.67313897593628E-4 78-79 7.626444543927177E-5 0.00204007391550052 0.0 10.28398400887108 6.67313897593628E-4 80-81 7.626444543927177E-5 0.002068673082540247 9.533055679908971E-6 10.996618053166994 6.76846953273537E-4 82-83 7.626444543927177E-5 0.0021163383609397917 1.9066111359817943E-5 11.662406661851838 7.054461203132639E-4 84-85 7.626444543927177E-5 0.0021258714166197006 1.9066111359817943E-5 12.299815363777592 7.054461203132639E-4 86-87 7.626444543927177E-5 0.0021449375279795186 1.9066111359817943E-5 12.937185933480626 7.054461203132639E-4 88-89 7.626444543927177E-5 0.0021544705836594276 1.9066111359817943E-5 13.607712470838383 7.054461203132639E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15875 0.0 34.669178 1 GGTATCA 10245 0.0 30.982286 1 TACATGG 17560 0.0 29.045792 2 ATCAACG 19330 0.0 29.038694 3 TATCAAC 19480 0.0 29.032644 2 GTACATG 17390 0.0 28.975052 1 TCAACGC 19580 0.0 28.668196 4 CAACGCA 19745 0.0 28.18832 5 AACGCAG 20445 0.0 27.316034 6 ACATGGG 19055 0.0 25.921846 3 ACGCAGA 22990 0.0 23.982557 7 CGCAGAG 23925 0.0 22.886866 8 CATGGGG 12550 0.0 22.760464 4 GCAGAGT 25140 0.0 20.535065 9 AGAGTAC 18895 0.0 20.063538 10-11 GAGTACT 11480 0.0 19.570143 12-13 GAGTACA 13080 0.0 19.1525 1 CATGGGA 6900 0.0 18.842123 4 AGTACTT 12200 0.0 18.784653 12-13 TAAGGTG 2190 0.0 18.632996 5 >>END_MODULE