FastQCFastQC Report
Fri 27 May 2016
SRR939879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244908
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115280.21979413175598123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85970.16391135936035484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58120.11081223922326187No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA54840.10455855469724158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97750.036.820481
GTATCAA155650.035.3674241
ATCAACG189800.028.600783
TCAACGC190650.028.5216544
CAACGCA193350.028.0989765
TATCAAC197200.027.6489522
AACGCAG199600.027.2951496
TACATGG176050.026.6036362
GTACATG179850.026.3857351
ACATGGG186650.024.206743
ACGCAGA232350.023.123337
CGCAGAG236300.022.6570198
CATGGGG123700.021.1213784
GAGTACA136550.020.3126811
GCAGAGT254350.020.307999
AGAGTAC203750.020.13535510-11
GAGTACT124400.019.85204512-13
TAAGGTG27100.019.4908565
AGTACTT130000.019.08751512-13
GTACTTT134800.018.23328814-15
Produced by FastQC (version 0.11.5)