##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939879_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5244908 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.419231185751972 31.0 31.0 34.0 30.0 34.0 2 31.634073657726695 31.0 31.0 34.0 30.0 34.0 3 31.700269289756847 31.0 31.0 34.0 30.0 34.0 4 35.51014698446569 37.0 35.0 37.0 33.0 37.0 5 35.234198197566094 37.0 35.0 37.0 33.0 37.0 6 35.25052679665687 37.0 35.0 37.0 32.0 37.0 7 35.16079271552523 37.0 35.0 37.0 32.0 37.0 8 35.09432653537488 37.0 35.0 37.0 32.0 37.0 9 36.71185500298575 39.0 35.0 39.0 32.0 39.0 10-11 36.566181904429975 38.5 35.0 39.0 32.0 39.0 12-13 36.50255247565829 38.0 35.0 39.0 32.0 39.0 14-15 37.56615406790739 39.0 36.0 41.0 32.0 41.0 16-17 37.486713970960025 39.0 36.0 41.0 32.0 41.0 18-19 37.43802093382763 39.0 36.0 41.0 32.0 41.0 20-21 37.34468469227677 39.0 36.0 41.0 31.5 41.0 22-23 37.2004326863312 39.0 36.0 40.0 31.0 41.0 24-25 37.030336280445724 39.0 36.0 40.0 31.0 41.0 26-27 36.727950995517936 39.0 35.5 40.0 30.0 41.0 28-29 36.50341950707238 38.0 35.0 40.0 30.0 41.0 30-31 36.25541820752623 38.0 35.0 40.0 30.0 41.0 32-33 36.21266788664358 38.0 35.0 40.0 30.0 41.0 34-35 36.30396863014566 38.0 35.0 40.0 30.0 41.0 36-37 36.27265616479831 38.0 35.0 40.0 30.0 41.0 38-39 36.13581305906605 38.0 35.0 40.0 29.5 41.0 40-41 35.962147572464566 38.0 35.0 40.0 29.0 41.0 42-43 35.75712481515405 38.0 34.5 40.0 28.5 41.0 44-45 35.52404093646638 38.0 34.0 40.0 27.5 41.0 46-47 35.24147306301655 38.0 34.0 40.0 27.0 41.0 48-49 34.93439332014975 38.0 33.5 40.0 26.0 41.0 50-51 34.644341426007855 38.0 33.0 40.0 26.0 41.0 52-53 34.32803616383738 37.0 33.0 40.0 24.5 41.0 54-55 33.97805032995812 37.0 33.0 40.0 24.0 41.0 56-57 32.53804528125183 36.5 31.0 39.5 16.0 40.5 58-59 28.960616182400152 34.0 23.0 38.0 7.0 40.0 60-61 28.926462485137968 33.5 24.0 38.0 8.5 40.0 62-63 30.89210306453421 34.5 29.0 38.0 9.0 40.0 64-65 30.44134434769876 35.0 29.0 38.0 4.0 40.0 66-67 24.949028085907322 28.5 17.0 35.5 2.0 38.0 68-69 17.269625511067115 13.0 7.0 29.0 2.0 35.0 70-71 16.12169746352081 12.5 7.5 25.5 2.0 33.0 72-73 18.20870204015018 17.5 7.0 30.0 2.0 34.0 74-75 18.9140248980535 17.5 7.5 30.5 2.0 35.0 76-77 19.838533869421543 22.5 7.5 30.5 2.0 34.5 78-79 22.889981864314876 27.5 11.0 33.5 2.0 35.0 80-81 25.596999699518086 31.5 21.0 34.5 2.0 36.0 82-83 26.690299334135126 32.5 25.0 35.0 2.0 36.0 84-85 26.7346686538639 33.0 25.0 35.0 2.0 35.0 86-87 26.480787365574383 33.0 25.0 35.0 2.0 35.0 88-89 26.054311248166794 33.0 24.0 35.0 2.0 35.0 90-91 24.36585894356965 31.5 11.0 34.0 2.0 35.0 92-93 22.16991451518311 30.5 2.0 34.0 2.0 35.0 94-95 17.748832772662553 20.0 2.0 33.0 2.0 35.0 96-97 16.42401163185322 13.5 2.0 32.5 2.0 34.5 98-99 15.336426301471828 2.0 2.0 32.0 2.0 34.0 100-101 14.716173381878194 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32778349612225455 1101 2 0.2916315539942751 1101 3 0.3497101618159526 1101 4 0.3244232931713853 1101 5 0.403449553826313 1101 6 0.3770571774657796 1101 7 0.38434873776861167 1101 8 0.39924184164091514 1101 9 0.473602440277503 1101 10-11 0.3704976254219261 1101 12-13 0.4136602236658504 1101 14-15 0.4443870494875881 1101 16-17 0.42785217119075014 1101 18-19 0.4855850661845693 1101 20-21 0.5285039572429895 1101 22-23 0.4493068366726547 1101 24-25 0.3552641673871406 1101 26-27 0.4456690261152545 1101 28-29 0.5098039265756782 1101 30-31 0.49795871952356663 1101 32-33 0.3347198675452674 1101 34-35 0.5893593631458884 1101 36-37 0.591992332224919 1101 38-39 0.5431039381904839 1101 40-41 0.5042286341887134 1101 42-43 0.4359900623644464 1101 44-45 0.26848057098348477 1101 46-47 0.12938055528500314 1101 48-49 0.15191667357240135 1101 50-51 0.1405467947476069 1101 52-53 0.26669913768663633 1101 54-55 0.1914379332832894 1101 56-57 0.737910492135704 1101 58-59 -8.365730804111188 1101 60-61 -9.736583055905676 1101 62-63 -3.078906472162384 1101 64-65 -0.4996096436597597 1101 66-67 -1.456477469974267 1101 68-69 -3.7428238239416594 1101 70-71 -4.458472590678385 1101 72-73 -7.025582275084494 1101 74-75 -7.828749600921592 1101 76-77 -8.84753019305865 1101 78-79 -11.962483628502964 1101 80-81 -8.210667485392023 1101 82-83 -4.414091725732575 1101 84-85 -3.438890487540256 1101 86-87 -3.3205217789646113 1101 88-89 -3.4693534441329597 1101 90-91 -9.129471222963613 1101 92-93 -14.543005629008174 1101 94-95 -12.404198176482739 1101 96-97 -12.273416727812055 1101 98-99 -11.816300166991393 1101 100-101 -11.594638437835004 1102 1 0.45770268552630355 1102 2 0.48345520700666 1102 3 0.4927962602656031 1102 4 0.3392785600056527 1102 5 0.4936996617756009 1102 6 0.43069710300889597 1102 7 0.3746902657235154 1102 8 0.4415956432902206 1102 9 0.6394072134285622 1102 10-11 0.5941157266562982 1102 12-13 0.647446726840947 1102 14-15 0.8204377921174881 1102 16-17 0.896650861868288 1102 18-19 0.8143628635522262 1102 20-21 0.8729735597640484 1102 22-23 0.7836536713733651 1102 24-25 0.7983945026738937 1102 26-27 0.8928699040068793 1102 28-29 0.8036317704958975 1102 30-31 0.7649384718422851 1102 32-33 0.6634111066157828 1102 34-35 0.6740149477338875 1102 36-37 0.6739248629105958 1102 38-39 0.6700155669313759 1102 40-41 0.6376360340020639 1102 42-43 0.828690638845849 1102 44-45 0.689854839378782 1102 46-47 0.6564528663690297 1102 48-49 0.7065273969940975 1102 50-51 0.6479294482032003 1102 52-53 0.6847156349444887 1102 54-55 0.5565630197042069 1102 56-57 1.2246845256304155 1102 58-59 -8.561763472070783 1102 60-61 -9.818841985684028 1102 62-63 -2.755710193478368 1102 64-65 -0.0116561879505781 1102 66-67 -1.164322624193261 1102 68-69 -3.61595586684464 1102 70-71 -4.029140584711827 1102 72-73 -6.614345401501108 1102 74-75 -7.632520240483267 1102 76-77 -8.63464597482896 1102 78-79 -11.621271867815617 1102 80-81 -7.69240967678958 1102 82-83 -3.49570269810096 1102 84-85 -2.5556280362417283 1102 86-87 -2.322519449217168 1102 88-89 -2.522587660366156 1102 90-91 -8.218384413417143 1102 92-93 -14.114862151147081 1102 94-95 -12.264890734965455 1102 96-97 -12.152107132175892 1102 98-99 -11.619518199334994 1102 100-101 -11.455501208446776 1103 1 0.273771072003548 1103 2 0.29730722248112684 1103 3 0.272269795162714 1103 4 0.21342158265976252 1103 5 0.24405474774047065 1103 6 0.2249626979785262 1103 7 0.30109998266727445 1103 8 0.3087540643568545 1103 9 0.34708665899052704 1103 10-11 0.2895596960974416 1103 12-13 0.30346542228031836 1103 14-15 0.4472118804540983 1103 16-17 0.4046131746957613 1103 18-19 0.48874267628520585 1103 20-21 0.39003174253579687 1103 22-23 0.4283235557441216 1103 24-25 0.3647513673348186 1103 26-27 0.27787180308687454 1103 28-29 0.42467700639475936 1103 30-31 0.3470379281081506 1103 32-33 0.2831741718895415 1103 34-35 0.32606336447508966 1103 36-37 0.1574496440381239 1103 38-39 0.10525734734454062 1103 40-41 0.12849641989325988 1103 42-43 0.2505797755016843 1103 44-45 0.11415556266229743 1103 46-47 0.03634632191539566 1103 48-49 0.24051066362632412 1103 50-51 0.15364109460290365 1103 52-53 0.16406862258926225 1103 54-55 0.07880657741360864 1103 56-57 0.5005085723692488 1103 58-59 -6.877716432279225 1103 60-61 -8.784362310272726 1103 62-63 -2.6610015109499514 1103 64-65 -0.41930939867893 1103 66-67 -1.9046709117317029 1103 68-69 -4.1392044904782495 1103 70-71 -4.760006945472634 1103 72-73 -7.698626058461469 1103 74-75 -8.359506085571532 1103 76-77 -9.09974145609176 1103 78-79 -12.221412234090756 1103 80-81 -8.75601036745745 1103 82-83 -4.8088890440424095 1103 84-85 -3.893632138161202 1103 86-87 -3.636956295008609 1103 88-89 -3.7222979690109845 1103 90-91 -9.24705603029012 1103 92-93 -14.735137198122482 1103 94-95 -12.649094857135557 1103 96-97 -12.469628524822113 1103 98-99 -12.160796301169542 1103 100-101 -11.83367009405077 1104 1 0.16419028186550122 1104 2 0.17660763459686635 1104 3 0.23816775828447945 1104 4 0.18184072206089752 1104 5 0.105848848408165 1104 6 0.16491626589835562 1104 7 0.19221038917016386 1104 8 0.18292865186078444 1104 9 0.2570179116478215 1104 10-11 0.26710681477028686 1104 12-13 0.1905626646198968 1104 14-15 0.26390785241709835 1104 16-17 0.3619418408003412 1104 18-19 0.32540738526199675 1104 20-21 0.2231225665350749 1104 22-23 0.3480202379201742 1104 24-25 0.3573699957348495 1104 26-27 0.3753918824193576 1104 28-29 0.31422106082253976 1104 30-31 0.25796734237854935 1104 32-33 0.2230267526372458 1104 34-35 0.21775867586788422 1104 36-37 0.15352805892585764 1104 38-39 0.2479524217327551 1104 40-41 0.3407053986837454 1104 42-43 0.2600507508476042 1104 44-45 0.05871720827478555 1104 46-47 0.12201967515391487 1104 48-49 0.14952612577325652 1104 50-51 0.1764801092403374 1104 52-53 0.09959196633959522 1104 54-55 -0.029949400746737354 1104 56-57 0.5789962229091898 1104 58-59 -6.663320665853647 1104 60-61 -8.862642581803115 1104 62-63 -2.681441754968592 1104 64-65 -0.3559768300056554 1104 66-67 -1.9955365469480206 1104 68-69 -4.482055739676557 1104 70-71 -5.044530245985639 1104 72-73 -7.89643861025618 1104 74-75 -8.701379090349254 1104 76-77 -9.229286779615025 1104 78-79 -12.138313403179914 1104 80-81 -8.622694989623302 1104 82-83 -4.780361118737286 1104 84-85 -3.8177271169468874 1104 86-87 -3.5954681824698653 1104 88-89 -3.760853650275049 1104 90-91 -9.204925963616567 1104 92-93 -15.014632758690365 1104 94-95 -12.689666178299987 1104 96-97 -12.444479416111474 1104 98-99 -11.770593378006268 1104 100-101 -11.368166070978054 1105 1 0.15351404654582623 1105 2 0.19222635541974853 1105 3 0.12932701212799813 1105 4 0.1191601449112838 1105 5 0.04122463861651937 1105 6 0.07869627940120694 1105 7 0.11203141942285555 1105 8 0.11022056617467513 1105 9 0.16425480888872102 1105 10-11 0.18791491406651062 1105 12-13 0.23319714167276828 1105 14-15 0.1912499833866974 1105 16-17 0.27477890082634104 1105 18-19 0.23671746338946065 1105 20-21 0.3048399493422096 1105 22-23 0.32615427012990494 1105 24-25 0.268984323773644 1105 26-27 0.2255165524744669 1105 28-29 0.2656538445885488 1105 30-31 0.21565377590315649 1105 32-33 0.21583916694543603 1105 34-35 0.2347936203580332 1105 36-37 0.14109866562216666 1105 38-39 0.18114640217037703 1105 40-41 0.14416984509037434 1105 42-43 0.0804181637914283 1105 44-45 0.16219543241538759 1105 46-47 0.16940837553479327 1105 48-49 0.2698198898568762 1105 50-51 0.27123602210069464 1105 52-53 0.222141591323755 1105 54-55 0.17727733918663802 1105 56-57 0.8265849183287557 1105 58-59 -6.6081648768923245 1105 60-61 -8.774834434904982 1105 62-63 -2.68677430789889 1105 64-65 -0.321694844376065 1105 66-67 -1.497583227653898 1105 68-69 -3.8257120820938084 1105 70-71 -4.637618684287872 1105 72-73 -5.949720426262083 1105 74-75 -6.622624523751176 1105 76-77 -8.433733266695132 1105 78-79 -11.680759551012653 1105 80-81 -7.886916158793092 1105 82-83 -3.9091541691275857 1105 84-85 -3.0088841583075805 1105 86-87 -2.8846005003406248 1105 88-89 -2.933290203977709 1105 90-91 -8.176680502726224 1105 92-93 -11.409158238111928 1105 94-95 -11.582569270881665 1105 96-97 -11.959318739574453 1105 98-99 -11.488835667671225 1105 100-101 -11.124230117662783 1106 1 0.02339741850816779 1106 2 0.07370920153358895 1106 3 0.10642131493275286 1106 4 0.10673285732622162 1106 5 0.031810139085145295 1106 6 -0.05873371838946184 1106 7 0.06825116975459622 1106 8 0.1090854360093374 1106 9 0.06951988071946147 1106 10-11 0.1511458633794973 1106 12-13 0.11349007030035807 1106 14-15 0.15533687738129487 1106 16-17 0.1173197288730563 1106 18-19 0.08252724537914702 1106 20-21 0.11473617934329639 1106 22-23 0.08180336780296926 1106 24-25 0.11913585514273706 1106 26-27 0.16278263428562667 1106 28-29 0.15659455352344764 1106 30-31 0.20337225621197064 1106 32-33 0.09607353454811829 1106 34-35 0.05479154139019471 1106 36-37 0.18154148577292517 1106 38-39 0.18935355656427078 1106 40-41 0.2255002678687248 1106 42-43 0.2310522832902322 1106 44-45 0.21088756952572396 1106 46-47 0.08016559325830741 1106 48-49 0.06195161408282246 1106 50-51 0.014200534188212544 1106 52-53 0.03691560820524131 1106 54-55 -0.25345680405219184 1106 56-57 0.6102289304210657 1106 58-59 -6.6787475848199875 1106 60-61 -9.089440556264556 1106 62-63 -2.9246335130607655 1106 64-65 -0.6395599022097933 1106 66-67 -1.8498944155198593 1106 68-69 -4.134852164731065 1106 70-71 -4.666167475536172 1106 72-73 -6.35325161832715 1106 74-75 -6.858432446333982 1106 76-77 -8.579361361990077 1106 78-79 -12.136032410396208 1106 80-81 -8.218452660116633 1106 82-83 -4.2431721209806135 1106 84-85 -3.3502283105072372 1106 86-87 -3.221897526524643 1106 88-89 -3.2869891886454887 1106 90-91 -8.454688968173503 1106 92-93 -11.84725803405746 1106 94-95 -11.672863029724141 1106 96-97 -12.050847151448858 1106 98-99 -11.69319210018985 1106 100-101 -11.425352657379303 1107 1 0.11442506417499487 1107 2 0.13591120556727532 1107 3 0.09505016134259492 1107 4 0.054467399327194244 1107 5 0.05284994171864099 1107 6 0.0697635806579413 1107 7 0.16852073580150062 1107 8 0.028418011889961292 1107 9 0.050700193408204086 1107 10-11 0.1444444695009679 1107 12-13 0.09733518967650667 1107 14-15 0.17175868816548245 1107 16-17 0.17416816926273526 1107 18-19 0.1466590610117393 1107 20-21 0.20818175055856614 1107 22-23 0.18466295531284516 1107 24-25 0.15817606608421642 1107 26-27 0.2720190318557556 1107 28-29 0.3529845559076179 1107 30-31 0.2705759482986281 1107 32-33 0.24692980077306714 1107 34-35 0.17855430305026943 1107 36-37 0.12280584219526247 1107 38-39 0.21852174471758445 1107 40-41 0.027279070645654713 1107 42-43 0.1819318077369374 1107 44-45 0.20668997953343649 1107 46-47 0.17408798176641227 1107 48-49 0.18619840186322278 1107 50-51 0.23939324565314735 1107 52-53 0.1997911270542403 1107 54-55 0.16039102448613818 1107 56-57 0.9245833716083709 1107 58-59 -6.473659987897726 1107 60-61 -8.85061445937276 1107 62-63 -2.680699224050631 1107 64-65 -0.2640805406249136 1107 66-67 -1.6393105710552156 1107 68-69 -4.169994723789511 1107 70-71 -4.63956220365448 1107 72-73 -6.026415813231049 1107 74-75 -6.7880877239352415 1107 76-77 -8.97778126503016 1107 78-79 -12.310392490413175 1107 80-81 -8.397796346751036 1107 82-83 -4.241405941284665 1107 84-85 -3.2639239564568534 1107 86-87 -3.035277300311975 1107 88-89 -3.116728183632489 1107 90-91 -8.372519003900791 1107 92-93 -11.77505126303818 1107 94-95 -12.047968075062068 1107 96-97 -12.4319765545498 1107 98-99 -11.818774352945386 1107 100-101 -11.373010107331043 1108 1 -0.011748612403472691 1108 2 0.03295673903023655 1108 3 0.028516950901870786 1108 4 0.04203342666793475 1108 5 -0.10592226965253104 1108 6 0.05438706202301802 1108 7 0.01966065772911918 1108 8 -0.028013536435267383 1108 9 -0.029556972835941053 1108 10-11 0.08556854113656698 1108 12-13 0.0017087382265188467 1108 14-15 0.14256044890537822 1108 16-17 0.082411829429752 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88-89 1.4541332841109487 2312 90-91 2.9527070100672574 2312 92-93 4.874247021397327 2312 94-95 8.866825251421233 2312 96-97 9.538053433251395 2312 98-99 10.03095235088631 2312 100-101 9.261605790428478 2313 1 -0.2073897714158015 2313 2 -0.2891022824794156 2313 3 -0.3083360001736146 2313 4 -0.2801368147419723 2313 5 -0.30795344794093893 2313 6 -0.3043099670134808 2313 7 -0.3387184690340277 2313 8 -0.3510860817952377 2313 9 -0.285553974335194 2313 10-11 -0.41525482269172187 2313 12-13 -0.4635829271652696 2313 14-15 -0.528985691289833 2313 16-17 -0.4480337386025539 2313 18-19 -0.44291428496926954 2313 20-21 -0.5732568063464925 2313 22-23 -0.5289520145281372 2313 24-25 -0.45742175691983533 2313 26-27 -0.4586132518470336 2313 28-29 -0.45887225650138674 2313 30-31 -0.5377835049942874 2313 32-33 -0.38571319458174713 2313 34-35 -0.4510451838997298 2313 36-37 -0.6172187860955916 2313 38-39 -0.7696283444673995 2313 40-41 -0.9236768946635934 2313 42-43 -0.6259649708889157 2313 44-45 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68-69 -0.3485253920699769 2314 70-71 2.3804843213257083 2314 72-73 8.178247416616955 2314 74-75 9.634321612813505 2314 76-77 8.643069627909636 2314 78-79 6.310640165119189 2314 80-81 3.2640750281507778 2314 82-83 1.7847120945657018 2314 84-85 1.489200633613713 2314 86-87 1.4843742111685359 2314 88-89 1.6032800488814303 2314 90-91 2.7230893939769487 2314 92-93 4.757228511958463 2314 94-95 8.749752011692141 2314 96-97 9.402684708592549 2314 98-99 9.597738418632169 2314 100-101 8.867745420452929 2315 1 -0.7242022895264952 2315 2 -0.5528619362659661 2315 3 -0.480658893348874 2315 4 -0.3755144620527773 2315 5 -0.5757380220404471 2315 6 -0.6310036159873107 2315 7 -0.5578225094507943 2315 8 -0.6461199094192409 2315 9 -0.9793194865370793 2315 10-11 -0.8343959664055802 2315 12-13 -0.6319934382962629 2315 14-15 -0.6807732323932285 2315 16-17 -0.7829986826011464 2315 18-19 -0.728442455757822 2315 20-21 -0.8504988155939657 2315 22-23 -0.8318155512471677 2315 24-25 -0.7943518842931212 2315 26-27 -0.8812637438279296 2315 28-29 -0.8241497438081495 2315 30-31 -0.8970603686632828 2315 32-33 -0.6606179480873493 2315 34-35 -0.8434566462357864 2315 36-37 -0.9024458469349241 2315 38-39 -0.8325554733399301 2315 40-41 -0.7652891872567906 2315 42-43 -0.7150983663760471 2315 44-45 -0.6040018557902513 2315 46-47 -0.6892227808576834 2315 48-49 -0.6163890210963956 2315 50-51 -0.6301983505714617 2315 52-53 -0.6336158221455364 2315 54-55 -0.6637570043104688 2315 56-57 0.6739664909047391 2315 58-59 4.293404329107204 2315 60-61 3.8534518277637133 2315 62-63 1.4298220437334628 2315 64-65 1.3149265694389989 2315 66-67 2.3044991081334274 2315 68-69 6.243585697292801 2315 70-71 5.1670076217517025 2315 72-73 9.0660023958572 2315 74-75 10.139881849850262 2315 76-77 8.868141939708252 2315 78-79 6.358248409320076 2315 80-81 3.3652876046539433 2315 82-83 1.6830343449503893 2315 84-85 1.2867064575899896 2315 86-87 1.1858051207199836 2315 88-89 1.277264885212194 2315 90-91 2.512479951297067 2315 92-93 4.601427579434574 2315 94-95 8.619445023544614 2315 96-97 9.26963902210452 2315 98-99 9.544210113528017 2315 100-101 8.775126241452428 2316 1 -0.4824527161339205 2316 2 -0.4699661759295175 2316 3 -0.576379240458234 2316 4 -0.40561939091420385 2316 5 -0.5238638657479129 2316 6 -0.5030027238096864 2316 7 -0.5859662340404128 2316 8 -0.6361326061521098 2316 9 -0.7365209511642377 2316 10-11 -0.7440324180210425 2316 12-13 -0.70738104794615 2316 14-15 -0.8362749633204771 2316 16-17 -0.9751241418136516 2316 18-19 -1.024207388417544 2316 20-21 -0.775438889907889 2316 22-23 -0.8245527260771226 2316 24-25 -2.311013254821681 2316 26-27 -1.532529964125807 2316 28-29 -1.2389288155234581 2316 30-31 -0.8450910218707648 2316 32-33 -0.8365807370010074 2316 34-35 -0.804744326391976 2316 36-37 -0.82913586327129 2316 38-39 -1.1203134384283473 2316 40-41 -0.9080542892996064 2316 42-43 -1.0123716485024943 2316 44-45 -0.9666221329532618 2316 46-47 -0.8586999003744893 2316 48-49 -1.0323222776240613 2316 50-51 -0.9937420689083751 2316 52-53 -0.9867135496589938 2316 54-55 -1.03971620024938 2316 56-57 0.050851811083120424 2316 58-59 3.6531782236725263 2316 60-61 3.511186219204781 2316 62-63 1.0895941651424117 2316 64-65 0.8023215274221904 2316 66-67 5.870215782942616 2316 68-69 8.731750228472588 2316 70-71 5.4319048183448135 2316 72-73 9.077365295173184 2316 74-75 10.028889376899063 2316 76-77 8.596069992488584 2316 78-79 5.957770427022712 2316 80-81 3.041005301361146 2316 82-83 1.6290472161991723 2316 84-85 1.3083565802410817 2316 86-87 1.174784837210943 2316 88-89 1.3354508949150912 2316 90-91 2.6289006158480746 2316 92-93 4.627457514980339 2316 94-95 8.498476796173708 2316 96-97 9.302648311110808 2316 98-99 9.688600803337177 2316 100-101 8.73396451605929 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 56.0 4 115.0 5 260.0 6 564.0 7 1550.0 8 3380.0 9 5703.0 10 9068.0 11 13644.0 12 19396.0 13 25512.0 14 33151.0 15 45629.0 16 60310.0 17 78144.0 18 87896.0 19 93123.0 20 98231.0 21 97905.0 22 94172.0 23 90589.0 24 93103.0 25 100739.0 26 132975.0 27 193281.0 28 270435.0 29 353131.0 30 411191.0 31 434011.0 32 441771.0 33 453312.0 34 455448.0 35 433118.0 36 352196.0 37 203775.0 38 56009.0 39 2006.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31209996737094 18.451440951784694 13.444986651832616 24.79147242901176 2 16.60385345065924 20.311631853412525 37.696433508432406 25.388081187495832 3 19.755429523681503 23.369302944588878 29.351736656303835 27.523530875425788 4 13.786361934279876 15.692382020809518 35.15003504351268 35.37122100139793 5 14.394456841603986 36.87765962318062 33.61086393108332 15.11701960413207 6 34.37183323625499 34.75569147582272 16.262067663761307 14.61040762416097 7 29.925615473140805 31.747134554123736 19.91140740695547 18.41584256577999 8 27.313570396548876 34.246512136928395 19.21580231623012 19.224115150292608 9 26.8956481219499 15.682162585120654 19.152843100393753 38.26934619253569 10-11 26.092545379251646 25.298222580834594 26.748724667811143 21.86050737210262 12-13 26.556786124751852 22.453463435392955 27.297304356911507 23.692446082943686 14-15 23.33120618597585 23.9866147965565 24.93791106630141 27.74426795116624 16-17 23.406559276158895 27.27677015497698 25.65367209491568 23.662998473948445 18-19 23.53293512501654 25.91184149865029 27.43361743554505 23.121605940788122 20-21 23.92962850825982 25.15575106369835 27.460405406539067 23.454215021502762 22-23 23.820484553780545 25.032031067084493 27.42947064085776 23.718013738277204 24-25 23.694695884084144 25.336221340774713 27.41828836654523 23.55079440859592 26-27 23.344298589515393 25.564674785474917 27.387054794853555 23.703971830156135 28-29 23.45017107960953 25.953107093804974 27.194436449723682 23.402285376861816 30-31 23.44215850856031 25.571100495839783 27.01610811915714 23.970632876442767 32-33 23.198881424793463 25.58563469390615 27.107764867743082 24.1077190135573 34-35 23.888571858380725 25.64399827564215 26.983025828569772 23.484404037407355 36-37 23.94495278119685 25.332785857893125 27.096220016470035 23.62604134443999 38-39 23.512329606254735 25.464111206276247 27.123598531291115 23.899960656177903 40-41 23.73767767994757 25.404102295436637 27.187512485978754 23.670707538637043 42-43 23.493973548361566 25.39303888693138 27.033194951502786 24.07979261320427 44-45 23.354507621046174 25.416011148684923 27.3368115440078 23.8926696862611 46-47 23.636975335478517 25.76146201775455 26.83916818664563 23.762394460121307 48-49 23.463888901603752 25.821329232027416 26.661692612614264 24.05308925375457 50-51 23.36188880739082 25.97818661583018 26.81378657448358 23.846138002295422 52-53 23.799811030577516 25.55045282386663 26.698028712710887 23.95170743284496 54-55 23.209234376567263 25.77378702650298 26.862113640428873 24.15486495650088 56-57 23.0631046062854 25.827945132240654 26.925491263118452 24.183458998355494 58-59 21.307328343189344 25.4684622682227 28.72346228839124 24.50074710019671 60-61 22.780494684780088 25.775089822076986 27.225352415693944 24.219063077448983 62-63 22.87563979000493 25.728409679144537 27.003308938136872 24.392641592713662 64-65 21.957593035458594 25.865233607369998 27.420079905536156 24.75709345163525 66-67 21.48350108072368 25.70423937546158 28.167757701364533 24.644501842450207 68-69 21.904361799951232 25.16253558449647 28.59363998429239 24.33946263125991 70-71 21.211541741793756 25.394035468525626 28.598835523233767 24.79558726644685 72-73 21.082912798875917 25.687095178139096 28.47462307244792 24.755368950537072 74-75 21.682092177470334 25.886369114516626 27.689454991098994 24.742083716914046 76-77 20.53803606312029 26.015839058754953 28.183772636796167 25.262352241328596 78-79 21.70623608401569 26.34071115825023 26.900356287488204 25.052696470245877 80-81 22.700564510716703 26.146571829069632 26.085216942337848 25.067646717875817 82-83 22.86538950291359 26.13633655379327 25.895369275656765 25.102904667636373 84-85 22.616187474820812 26.270885260691944 25.79142013385557 25.321507130631677 86-87 22.76229594543514 26.195584456317654 25.734429600549504 25.307689997697707 88-89 22.70099051355644 26.303143116687234 25.396358428192844 25.599507941563488 90-91 22.103777540856566 26.325021781029452 25.827461762696984 25.743738915416998 92-93 21.894511821361633 26.196271453722815 26.056785431116086 25.852431293799466 94-95 20.132826435907646 26.18548100945002 27.485108077772548 26.196584476869788 96-97 20.00310418807673 25.90642822718526 27.668248007253144 26.422219577484867 98-99 20.40170745161691 26.201103066115618 26.91685415324898 26.480335329018494 100-101 20.414058273159515 26.089093716110508 26.82878018345442 26.668067827275564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 379.0 1 296.0 2 245.0 3 331.0 4 378.5 5 437.5 6 623.5 7 901.5 8 1274.5 9 1623.0 10 1815.5 11 1989.5 12 2270.0 13 2615.5 14 2944.0 15 3224.0 16 3596.0 17 4067.0 18 4588.5 19 5290.0 20 6017.0 21 6998.5 22 8471.5 23 10216.0 24 12535.0 25 15510.0 26 19247.5 27 23665.0 28 28536.0 29 34116.0 30 40499.0 31 49156.5 32 58760.5 33 65633.5 34 70712.5 35 76307.5 36 83808.5 37 92579.5 38 101795.0 39 111663.0 40 122925.5 41 135459.5 42 148548.0 43 162390.5 44 176677.5 45 190518.5 46 204060.5 47 216834.5 48 226501.0 49 231445.5 50 233147.5 51 233614.0 52 232780.0 53 230629.5 54 224284.5 55 212180.5 56 195533.5 57 176114.0 58 155810.0 59 133374.0 60 109364.0 61 86959.0 62 67326.0 63 50279.0 64 36685.0 65 26509.0 66 18940.5 67 13816.0 68 10077.5 69 7379.5 70 5481.0 71 4041.0 72 3049.5 73 2363.0 74 1717.0 75 1159.0 76 779.5 77 485.5 78 291.0 79 178.0 80 102.5 81 61.5 82 37.5 83 25.5 84 15.5 85 10.0 86 6.0 87 4.5 88 5.0 89 1.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02129684638891664 2 0.004919056730833029 3 0.003870420606043042 4 0.0 5 0.06795162088639115 6 5.719833407945382E-5 7 0.0 8 1.1439666815890764E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.287933363178153E-4 16-17 0.0 18-19 0.0015634211315050713 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.620619465584525E-4 28-29 0.0024309291983767877 30-31 0.008999204561834069 32-33 4.099213942360858E-4 34-35 0.0010867683475096225 36-37 0.02756959702629674 38-39 0.026520960901506758 40-41 0.02910441899076209 42-43 0.02984799733379499 44-45 0.030486712064348886 46-47 0.0321931290310526 48-49 0.032412389311690507 50-51 0.027264539244539657 52-53 0.022926998910181077 54-55 0.01704510355567724 56-57 0.1481532183214653 58-59 0.73936473242238 60-61 0.3016354147679997 62-63 4.3852056127581263E-4 64-65 0.14010731932762216 66-67 1.3813016357960903 68-69 2.652153288484755 70-71 2.13953228540901 72-73 1.8134159836550041 74-75 1.5207702403931584 76-77 1.0910772886769415 78-79 0.4117898731493479 80-81 2.287933363178153E-4 82-83 9.53305567990897E-5 84-85 1.906611135981794E-5 86-87 0.0026787886460544206 88-89 4.766527839954485E-5 90-91 0.22058537537741366 92-93 0.16967885804669977 94-95 1.2649506912228012 96-97 1.2356651441741209 98-99 0.976242099956758 100-101 0.3940488565290373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5244908.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.885847206209135 #Duplication Level Percentage of deduplicated Percentage of total 1 62.70780526317673 19.9949149725837 2 14.343759152443452 9.147258253949515 3 6.7844984385544755 6.489884417475375 4 4.186750750019767 5.339923788224472 5 2.5849507034121637 4.121167158229154 6 1.769618156781676 3.385546449628437 7 1.3481720413276388 3.009132540221931 8 0.9374620430821132 2.3913417173869527 9 0.7538005644557488 2.163201265997119 >10 4.142151731369698 23.740441110178633 >50 0.2762607447946679 6.048602751319089 >100 0.15129513356663277 8.89377833772772 >500 0.008384618894318883 1.800511090960234 >1k 0.004851097738823029 2.874266139592701 >5k 1.7967028662287424E-4 0.37988596543077613 >10k+ 5.9890095540958084E-5 0.22014404109415306 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11528 0.21979413175598123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8597 0.16391135936035484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5812 0.11081223922326187 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5484 0.10455855469724158 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.9066111359817943E-5 0.0 0.0 0.0 5 0.0 1.9066111359817943E-5 0.0 0.0 0.0 6 0.0 1.9066111359817943E-5 0.0 0.0 0.0 7 0.0 1.9066111359817943E-5 0.0 0.0 0.0 8 0.0 1.9066111359817943E-5 0.0 0.0 0.0 9 0.0 1.9066111359817943E-5 0.0 0.0 7.626444543927177E-5 10-11 0.0 1.9066111359817943E-5 0.0 0.0 7.626444543927177E-5 12-13 0.0 3.8132222719635886E-5 0.0 0.0 7.626444543927177E-5 14-15 0.0 1.8112805791827046E-4 0.0 2.8599167039726914E-5 9.53305567990897E-5 16-17 0.0 2.8599167039726914E-4 0.0 7.626444543927177E-5 1.1439666815890764E-4 18-19 0.0 3.1459083743699606E-4 0.0 7.626444543927177E-5 1.715950022383615E-4 20-21 0.0 3.43190004476723E-4 0.0 1.2392972383881663E-4 1.715950022383615E-4 22-23 0.0 3.43190004476723E-4 0.0 4.289875055959037E-4 1.9066111359817943E-4 24-25 0.0 3.9085528287626783E-4 0.0 9.723716793507151E-4 2.0972722495799735E-4 26-27 0.0 4.3852056127581263E-4 0.0 0.0021354044722996096 2.383263919977243E-4 28-29 0.0 4.480536169557216E-4 0.0 0.01000970846390442 2.4785944767763326E-4 30-31 0.0 4.575866726356306E-4 0.0 0.03949544968186287 2.4785944767763326E-4 32-33 0.0 4.575866726356306E-4 0.0 0.10405330274620642 2.4785944767763326E-4 34-35 0.0 5.529172294347203E-4 0.0 0.19601869089028826 2.669255590374512E-4 36-37 0.0 6.387147305539011E-4 0.0 0.3482615900984345 2.669255590374512E-4 38-39 0.0 8.007766771123535E-4 0.0 0.5904107374238023 2.669255590374512E-4 40-41 0.0 9.342394566310791E-4 0.0 0.8626938737533623 2.764586147173602E-4 42-43 0.0 0.0010677022361498048 0.0 1.1362353734326702 2.8599167039726914E-4 44-45 0.0 0.0010867683475096227 0.0 1.4551351520369853 2.8599167039726914E-4 46-47 0.0 0.0011439666815890766 0.0 1.8089354474854469 2.8599167039726914E-4 48-49 0.0 0.0012106980713484394 0.0 2.1491034733116385 3.050577817570871E-4 50-51 0.0 0.0012583633497479842 0.0 2.506459598528706 3.050577817570871E-4 52-53 0.0 0.0012774294611078022 0.0 2.836846709227311 3.33656948796814E-4 54-55 0.0 0.001306028628147529 0.0 3.1947080863954143 3.6225611583654086E-4 56-57 0.0 0.001315561683827438 0.0 3.5014246198408054 3.6225611583654086E-4 58-59 0.0 0.001315561683827438 0.0 3.709054572549223 3.813222271963588E-4 60-61 0.0 0.001353693906547074 1.9066111359817943E-5 3.865501549312209 3.813222271963588E-4 62-63 9.533055679908971E-6 0.001353693906547074 3.8132222719635886E-5 3.9974009076994292 3.908552828762678E-4 64-65 1.9066111359817943E-5 0.001363226962226983 3.8132222719635886E-5 4.139119313436956 4.0038833855617675E-4 66-67 1.9066111359817943E-5 0.0013918261292667098 3.8132222719635886E-5 4.256147104963519 4.0038833855617675E-4 68-69 1.9066111359817943E-5 0.0013918261292667098 3.8132222719635886E-5 4.384299972468535 4.0038833855617675E-4 70-71 1.9066111359817943E-5 0.0013918261292667098 3.8132222719635886E-5 4.61214953627404 4.0038833855617675E-4 72-73 3.8132222719635886E-5 0.0013918261292667098 3.8132222719635886E-5 4.907769592908017 4.0038833855617675E-4 74-75 3.8132222719635886E-5 0.0013918261292667098 3.8132222719635886E-5 5.251569712948254 4.0038833855617675E-4 76-77 3.8132222719635886E-5 0.0014013591849466188 3.8132222719635886E-5 5.744991141884662 4.099213942360857E-4 78-79 3.8132222719635886E-5 0.0014108922406265278 3.8132222719635886E-5 6.4321814605709005 4.194544499159947E-4 80-81 3.8132222719635886E-5 0.0014204252963064368 3.8132222719635886E-5 7.19565529080777 4.3852056127581263E-4 82-83 3.8132222719635886E-5 0.0014299583519863455 3.8132222719635886E-5 7.909528632342074 4.957188953552665E-4 84-85 3.8132222719635886E-5 0.0014299583519863455 3.8132222719635886E-5 8.490596975199566 4.957188953552665E-4 86-87 3.8132222719635886E-5 0.0014299583519863455 3.8132222719635886E-5 9.003237044386669 4.957188953552665E-4 88-89 3.8132222719635886E-5 0.0014299583519863455 3.8132222719635886E-5 9.51724987359168 5.147850067150845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9775 0.0 36.82048 1 GTATCAA 15565 0.0 35.367424 1 ATCAACG 18980 0.0 28.60078 3 TCAACGC 19065 0.0 28.521654 4 CAACGCA 19335 0.0 28.098976 5 TATCAAC 19720 0.0 27.648952 2 AACGCAG 19960 0.0 27.295149 6 TACATGG 17605 0.0 26.603636 2 GTACATG 17985 0.0 26.385735 1 ACATGGG 18665 0.0 24.20674 3 ACGCAGA 23235 0.0 23.12333 7 CGCAGAG 23630 0.0 22.657019 8 CATGGGG 12370 0.0 21.121378 4 GAGTACA 13655 0.0 20.312681 1 GCAGAGT 25435 0.0 20.30799 9 AGAGTAC 20375 0.0 20.135355 10-11 GAGTACT 12440 0.0 19.852045 12-13 TAAGGTG 2710 0.0 19.490856 5 AGTACTT 13000 0.0 19.087515 12-13 GTACTTT 13480 0.0 18.233288 14-15 >>END_MODULE