FastQCFastQC Report
Fri 27 May 2016
SRR939878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5443119
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101110.18575746736384047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66940.12298095999738386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163350.035.938771
GGTATCA104750.031.5695931
ATCAACG204500.028.4783633
TATCAAC204800.028.4590912
GTACATG182100.028.3893531
TCAACGC206250.028.2828664
TACATGG181750.027.7977452
CAACGCA209450.027.7392225
AACGCAG214200.027.3217546
ACATGGG197400.024.450513
ACGCAGA244500.023.6472537
CGCAGAG253400.022.723888
CATGGGG131700.021.7176824
GCAGAGT262000.020.3978659
GAGTACA133850.020.1725711
GAGTACT120200.020.11668212-13
AGAGTAC196550.020.02094710-11
TAAGGTG22850.019.9706955
AGTACTT126100.018.63451612-13
GTATAGG12450.018.5137521
Produced by FastQC (version 0.11.5)