FastQCFastQC Report
Fri 27 May 2016
SRR939878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5443119
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118840.21833070340736624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88620.16281106475901042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60080.11037789179328984No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA56010.10290056124071512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA164200.035.0297281
GGTATCA106400.033.4969751
ATCAACG201350.028.2943153
TCAACGC202750.028.0755424
TATCAAC205850.027.8204352
GTACATG189650.027.7764951
CAACGCA205150.027.7702165
TACATGG185050.027.7425082
AACGCAG211600.026.946146
ACATGGG200550.024.882893
ACGCAGA243050.023.3422837
CGCAGAG252700.022.4321278
GAGTACA140200.021.190131
CATGGGG132800.020.7889584
GAGTACT124200.020.41427212-13
AGAGTAC204400.020.19045610-11
GCAGAGT273150.020.0580439
CATGGGA73800.019.540024
GTATAGA12200.019.0609071
AGTACTT130600.018.99618112-13
Produced by FastQC (version 0.11.5)