##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939878_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5443119 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.382964620101085 31.0 31.0 34.0 30.0 34.0 2 31.618485467615166 31.0 31.0 34.0 30.0 34.0 3 31.668265749839385 31.0 31.0 34.0 30.0 34.0 4 35.483599384837994 37.0 35.0 37.0 33.0 37.0 5 35.20912109399041 37.0 35.0 37.0 32.0 37.0 6 35.22519404775093 37.0 35.0 37.0 32.0 37.0 7 35.12118750297394 37.0 35.0 37.0 32.0 37.0 8 35.07956816670736 37.0 35.0 37.0 32.0 37.0 9 36.67105845012758 39.0 35.0 39.0 32.0 39.0 10-11 36.53447279032481 38.0 35.0 39.0 32.0 39.0 12-13 36.44960573156677 38.0 35.0 39.0 32.0 39.0 14-15 37.50046590934352 39.0 36.0 41.0 32.0 41.0 16-17 37.41508094899267 39.0 36.0 41.0 32.0 41.0 18-19 37.368666659685374 39.0 36.0 41.0 32.0 41.0 20-21 37.26425428141475 39.0 36.0 40.5 31.0 41.0 22-23 37.13536448495798 39.0 36.0 40.0 31.0 41.0 24-25 36.97165421149161 39.0 36.0 40.0 31.0 41.0 26-27 36.65945278800629 38.5 35.5 40.0 30.0 41.0 28-29 36.43957425880272 38.0 35.0 40.0 30.0 41.0 30-31 36.17603436559076 38.0 35.0 40.0 30.0 41.0 32-33 36.16201969863235 38.0 35.0 40.0 30.0 41.0 34-35 36.012750869492294 38.0 35.0 40.0 29.5 41.0 36-37 36.06668814332371 38.0 35.0 40.0 30.0 41.0 38-39 35.9668832336754 38.0 35.0 40.0 29.0 41.0 40-41 35.87943493427206 38.0 35.0 40.0 29.0 41.0 42-43 35.65241344163154 38.0 34.0 40.0 28.0 41.0 44-45 35.421889545314 38.0 34.0 40.0 27.0 41.0 46-47 35.157261856667105 38.0 34.0 40.0 26.5 41.0 48-49 34.85331553471457 38.0 33.0 40.0 26.0 41.0 50-51 34.6087017388376 38.0 33.0 40.0 26.0 41.0 52-53 34.3368414322744 37.0 33.0 40.0 24.5 41.0 54-55 34.04246692016103 37.0 33.0 40.0 24.0 41.0 56-57 33.639619949517915 36.5 32.0 39.5 23.5 41.0 58-59 33.24156131806047 36.0 31.5 39.0 23.0 40.5 60-61 32.89324741935644 36.0 31.0 39.0 22.0 40.0 62-63 32.57057093552429 35.0 31.0 39.0 22.0 40.0 64-65 32.10108717079307 35.0 31.0 38.0 20.5 40.0 66-67 31.174419298935042 34.5 29.5 37.5 15.5 40.0 68-69 29.27006675768066 34.0 26.0 36.5 8.5 39.0 70-71 28.610416288896126 33.0 25.5 36.0 8.5 39.0 72-73 29.17164230655255 33.0 26.5 35.5 9.0 38.5 74-75 29.274332051164045 33.0 27.5 35.0 8.0 37.5 76-77 28.81081196277355 32.5 27.0 35.0 8.0 36.5 78-79 29.35293744266844 33.5 29.0 35.0 7.5 37.0 80-81 29.190719512103264 34.0 29.0 35.0 7.0 36.0 82-83 28.77797160047392 33.0 28.5 35.0 4.5 36.0 84-85 28.450180953236554 33.0 27.5 35.0 2.0 35.5 86-87 28.092220655106015 33.0 27.0 35.0 2.0 35.0 88-89 27.652220721244564 33.0 26.5 35.0 2.0 35.0 90-91 27.1788133788734 32.5 25.5 35.0 2.0 35.0 92-93 26.699550202742216 32.0 25.0 34.0 2.0 35.0 94-95 25.893463288236028 32.0 23.5 34.0 2.0 35.0 96-97 25.356052660248654 32.0 21.5 34.0 2.0 35.0 98-99 24.664777951758907 31.5 18.5 34.0 2.0 35.0 100-101 23.178296579589755 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28779956621045955 1101 2 0.35534900841415507 1101 3 0.31687571118868973 1101 4 0.22917147028228868 1101 5 0.25692421325327786 1101 6 0.19093395438937932 1101 7 0.21484194712357407 1101 8 0.19851350299155968 1101 9 0.3494940555743824 1101 10-11 0.3658022272872685 1101 12-13 0.44593707555014106 1101 14-15 0.4691898151325731 1101 16-17 0.442918176839072 1101 18-19 0.3573061403671218 1101 20-21 0.42732291914407483 1101 22-23 0.25847071914509456 1101 24-25 0.2695684137068284 1101 26-27 0.3684837482123413 1101 28-29 0.30845325038500704 1101 30-31 0.17188147804593967 1101 32-33 0.15806984789669087 1101 34-35 0.16044662096493312 1101 36-37 0.31901537218331555 1101 38-39 0.3448358664524207 1101 40-41 0.26492753719189466 1101 42-43 0.17019847074563899 1101 44-45 0.0659850783292768 1101 46-47 0.02084557398298159 1101 48-49 0.19437317472428361 1101 50-51 0.1391778020300336 1101 52-53 -0.13997585747275565 1101 54-55 -0.2235483612109519 1101 56-57 -0.1846689886843791 1101 58-59 -0.14288869799982962 1101 60-61 -0.25103864237267004 1101 62-63 -0.13953270252884664 1101 64-65 -0.2697809505910236 1101 66-67 -0.43258935095641604 1101 68-69 -8.94432536299508 1101 70-71 -6.706362081224764 1101 72-73 -1.8634483491421499 1101 74-75 -0.7139477826698517 1101 76-77 -0.5379723251829525 1101 78-79 -0.7107923748303158 1101 80-81 -0.8730226076381378 1101 82-83 -0.6693661490179998 1101 84-85 -0.6613654495618562 1101 86-87 -0.7132876040924643 1101 88-89 -0.5690274523895269 1101 90-91 -0.86346460036415 1101 92-93 -0.981297142081548 1101 94-95 -0.6571855623535612 1101 96-97 -1.0967539316932537 1101 98-99 -1.1159615923977348 1101 100-101 -1.1612257221316185 1102 1 0.18796351119807042 1102 2 0.08595165061349519 1102 3 0.23500109293430782 1102 4 0.15500107569515365 1102 5 0.2222665292035373 1102 6 0.249214886676711 1102 7 0.29855094039989893 1102 8 0.1919037716289722 1102 9 0.2624072889681983 1102 10-11 0.30385958277251035 1102 12-13 0.21072543791323284 1102 14-15 0.337018624325772 1102 16-17 0.33521503035351685 1102 18-19 0.24734108441631264 1102 20-21 0.17056329433643924 1102 22-23 0.29512578368169784 1102 24-25 0.22794855683640236 1102 26-27 0.2819153537829564 1102 28-29 0.1267728965132804 1102 30-31 0.17222743232360216 1102 32-33 0.12160590567602725 1102 34-35 0.009911702176168546 1102 36-37 -0.0250948082917688 1102 38-39 0.14067667524310679 1102 40-41 0.11287931579437327 1102 42-43 0.17691425116136372 1102 44-45 0.1226960785361797 1102 46-47 0.04269907809974427 1102 48-49 0.03805346334564064 1102 50-51 0.05218087512957936 1102 52-53 -0.010554990267856112 1102 54-55 -0.1183538091209968 1102 56-57 -0.09627473688376398 1102 58-59 -0.18054433698744532 1102 60-61 -0.09299007159660988 1102 62-63 -0.08157168976055829 1102 64-65 -0.17398389671390646 1102 66-67 0.20134361695651037 1102 68-69 -4.518521258912212 1102 70-71 0.17065250663726417 1102 72-73 0.4857425325458564 1102 74-75 0.25650212962071706 1102 76-77 -0.0012502035053323368 1102 78-79 -0.1028065455855689 1102 80-81 -0.14315814462593934 1102 82-83 -0.16576555738714305 1102 84-85 -0.21712018430879 1102 86-87 -0.40317320877408136 1102 88-89 -0.5243108702477812 1102 90-91 -0.5697448070508848 1102 92-93 -0.6120032453447948 1102 94-95 -0.34075296302261293 1102 96-97 -0.8953260671436531 1102 98-99 -0.609141036274643 1102 100-101 -0.6069687345262977 1103 1 0.18293002277547998 1103 2 0.17395948072474 1103 3 0.13001101949862104 1103 4 0.13272703086035165 1103 5 0.16461251501071672 1103 6 0.19069899508113508 1103 7 0.11305324287472018 1103 8 0.17123703451942873 1103 9 0.21814803538012484 1103 10-11 0.301155221864839 1103 12-13 0.2646247689560255 1103 14-15 0.2860717591453934 1103 16-17 0.30753806070607936 1103 18-19 0.25714826089607357 1103 20-21 0.22619374896950006 1103 22-23 0.17963245402059158 1103 24-25 0.07139412757818775 1103 26-27 0.0930005513343346 1103 28-29 0.061490770420114416 1103 30-31 -0.004379674281146606 1103 32-33 0.07711294668506952 1103 34-35 -0.042373408680433045 1103 36-37 0.039133868895774526 1103 38-39 0.08507422537230269 1103 40-41 0.14867824112810268 1103 42-43 0.022421543655539722 1103 44-45 0.06493741407906839 1103 46-47 -0.09796049371166049 1103 48-49 -0.08022362756900492 1103 50-51 -0.1411533728795149 1103 52-53 -0.13090379135643104 1103 54-55 -0.19298269785349476 1103 56-57 -0.11571761348967158 1103 58-59 -0.18508555340615374 1103 60-61 -0.36188764122942274 1103 62-63 -0.39313290066687756 1103 64-65 -0.5363262765487988 1103 66-67 -0.31493477221632205 1103 68-69 -4.701146245839382 1103 70-71 -0.12231068587724181 1103 72-73 0.19896842629325207 1103 74-75 0.057948635589735176 1103 76-77 -0.10226594992233018 1103 78-79 -0.3466904373341997 1103 80-81 -0.2872871802947117 1103 82-83 -0.40825784737356585 1103 84-85 -0.4009097603447458 1103 86-87 -0.4084424983089825 1103 88-89 -0.46552787710555066 1103 90-91 -0.5757945962394153 1103 92-93 -0.5556636465839127 1103 94-95 -0.7560815361209947 1103 96-97 -1.0125798772278962 1103 98-99 -0.6854384477287212 1103 100-101 -0.8341392928915674 1104 1 0.1729163046026443 1104 2 0.18528793323812565 1104 3 0.2064112869207939 1104 4 0.14224070245350617 1104 5 0.2550067800472746 1104 6 0.2482226182862135 1104 7 0.1799096989098956 1104 8 0.18659417280993296 1104 9 0.24153103544040277 1104 10-11 0.2863807862553145 1104 12-13 0.13464941965408173 1104 14-15 0.32664836993470914 1104 16-17 0.3245627059241727 1104 18-19 0.21800443197319908 1104 20-21 0.27923932947106067 1104 22-23 0.27363025107565875 1104 24-25 0.1676747856851577 1104 26-27 0.26576344796945506 1104 28-29 0.15882883296110606 1104 30-31 0.2515921148718121 1104 32-33 0.21736893400078827 1104 34-35 0.1722790064303723 1104 36-37 0.29179005239414124 1104 38-39 0.267420663122671 1104 40-41 0.2920082590841133 1104 42-43 0.2878063100904029 1104 44-45 0.22901525511996823 1104 46-47 0.20836347436572566 1104 48-49 0.2545598408454097 1104 50-51 0.2572147856156164 1104 52-53 0.15658280556858273 1104 54-55 0.15778604955493591 1104 56-57 0.290473191498549 1104 58-59 0.0999836950535169 1104 60-61 0.05507882505327899 1104 62-63 0.19319728716796902 1104 64-65 0.23468298641450502 1104 66-67 0.38427625018028166 1104 68-69 -4.102651226211982 1104 70-71 0.6389335122124429 1104 72-73 0.9206893080557528 1104 74-75 0.6285860374299084 1104 76-77 0.48557853422139985 1104 78-79 0.2217905992555096 1104 80-81 0.12127530680454157 1104 82-83 0.10265504131560732 1104 84-85 0.027174864889751404 1104 86-87 0.23966833027961698 1104 88-89 0.14488537600113105 1104 90-91 0.12202725565911621 1104 92-93 0.06241698741121482 1104 94-95 0.4879019216988212 1104 96-97 -0.036439405695386284 1104 98-99 0.11154994753731984 1104 100-101 0.195739061073823 1105 1 0.15167490033888953 1105 2 0.17570430079017285 1105 3 0.1402352323853826 1105 4 0.11197367088303878 1105 5 0.22091775327162821 1105 6 0.2366035308921397 1105 7 0.16454755690919853 1105 8 0.17782201507244366 1105 9 0.2257772377272076 1105 10-11 0.2251675965996398 1105 12-13 0.2093567845136164 1105 14-15 0.21339752808342638 1105 16-17 0.2938756147809798 1105 18-19 0.1758966955379222 1105 20-21 0.2173631799246536 1105 22-23 0.23452402160069852 1105 24-25 0.22087006759707606 1105 26-27 0.2432419453972301 1105 28-29 0.3168072383289413 1105 30-31 0.28104112809828763 1105 32-33 0.2230036439991423 1105 34-35 0.2660065808529879 1105 36-37 0.18231636261241135 1105 38-39 0.2532720924896026 1105 40-41 0.19765575162148252 1105 42-43 0.20479183454659022 1105 44-45 0.18939159744694933 1105 46-47 0.12854622137025018 1105 48-49 0.09671612526765472 1105 50-51 0.1018193993904788 1105 52-53 0.03318379853289599 1105 54-55 0.03801426839714139 1105 56-57 0.041071484893272725 1105 58-59 0.10583936495158497 1105 60-61 0.19631191184559782 1105 62-63 0.27995648530342265 1105 64-65 0.2535649937959832 1105 66-67 0.7176168809480643 1105 68-69 1.829852669495331 1105 70-71 2.1603567428813015 1105 72-73 1.2249772965748882 1105 74-75 0.7308517865765083 1105 76-77 0.45663015707458854 1105 78-79 0.3209773763684467 1105 80-81 0.33573579063567394 1105 82-83 0.3951138244435555 1105 84-85 0.3228135652214874 1105 86-87 0.30062922116324486 1105 88-89 0.39196786170938225 1105 90-91 0.3075923369060263 1105 92-93 0.31936868384820727 1105 94-95 0.6191955018169537 1105 96-97 0.25301718082459956 1105 98-99 0.4059676507604486 1105 100-101 0.40111627588527554 1106 1 0.4317568058833352 1106 2 0.3631527833679158 1106 3 0.3495497028671437 1106 4 0.2432026792808557 1106 5 0.34158355139531693 1106 6 0.4070840856078348 1106 7 0.3450878080500388 1106 8 0.33943412324455124 1106 9 0.40022379270206443 1106 10-11 0.4049046129034082 1106 12-13 0.4608901278248112 1106 14-15 0.6346687950813745 1106 16-17 0.5916914167049683 1106 18-19 0.6003183728169574 1106 20-21 0.5756384959936085 1106 22-23 0.555982775352021 1106 24-25 0.5938288660478932 1106 26-27 0.5929119225050599 1106 28-29 0.5439098137898881 1106 30-31 0.6840775630407734 1106 32-33 0.6867017620332589 1106 34-35 0.7153178235960098 1106 36-37 0.6718073107137883 1106 38-39 0.8444339813328341 1106 40-41 0.7471016285703058 1106 42-43 0.6638797606273599 1106 44-45 0.823448777976985 1106 46-47 0.8595937196885757 1106 48-49 0.8796655451954507 1106 50-51 0.8591891572866288 1106 52-53 0.8533156804552462 1106 54-55 0.8007300690152945 1106 56-57 0.8863419938413699 1106 58-59 0.8885226471065124 1106 60-61 1.0467049493051164 1106 62-63 0.9851382113467579 1106 64-65 1.0436784534999717 1106 66-67 1.4446631886191987 1106 68-69 2.934542516951197 1106 70-71 3.304962669130145 1106 72-73 2.1653220217500113 1106 74-75 1.6535515288596514 1106 76-77 1.318060035287722 1106 78-79 1.2074161861096542 1106 80-81 1.2033602820742964 1106 82-83 1.3012205956300242 1106 84-85 1.2862439215685306 1106 86-87 1.273581641146965 1106 88-89 1.353086601310892 1106 90-91 1.284224697129705 1106 92-93 1.1867277573934416 1106 94-95 1.6321670438088631 1106 96-97 1.4313612135761034 1106 98-99 1.6304546864005083 1106 100-101 1.8796296979546376 1107 1 0.07774579165815965 1107 2 0.03654283781068912 1107 3 0.03851448802891966 1107 4 0.06506307863926253 1107 5 0.1179162362104691 1107 6 0.08608252441812425 1107 7 0.08696171699701694 1107 8 0.12901633203220086 1107 9 0.08393337465015094 1107 10-11 0.14102068640600152 1107 12-13 0.0724929158402503 1107 14-15 0.14752980975776353 1107 16-17 0.04504976271955741 1107 18-19 0.08954721662411913 1107 20-21 0.16813896976505305 1107 22-23 0.1686240168972546 1107 24-25 0.15316298237140558 1107 26-27 0.12270206932908678 1107 28-29 0.12167522690354104 1107 30-31 0.1538790369295313 1107 32-33 0.18848839008072815 1107 34-35 0.24095131534336645 1107 36-37 0.08738269318187264 1107 38-39 0.25499913468271274 1107 40-41 0.08856199437199308 1107 42-43 0.1251807219739547 1107 44-45 0.11004993661973117 1107 46-47 0.13453530458858864 1107 48-49 0.2364007870060547 1107 50-51 0.1444394907649098 1107 52-53 0.13599733712786843 1107 54-55 0.0998675543133416 1107 56-57 0.07286248600216538 1107 58-59 0.18997447666644263 1107 60-61 0.2908005872989463 1107 62-63 0.20564567988983384 1107 64-65 0.07219048696043728 1107 66-67 0.5789963941651486 1107 68-69 1.9234462549494005 1107 70-71 2.2736577738929604 1107 72-73 1.3393039980470682 1107 74-75 0.642562972570758 1107 76-77 0.28517219194547394 1107 78-79 0.3484232687838613 1107 80-81 0.4008154024657209 1107 82-83 0.4970399261065843 1107 84-85 0.4561416370710347 1107 86-87 0.3689008795430766 1107 88-89 0.36853719889795045 1107 90-91 0.420295950349594 1107 92-93 0.40730529731808574 1107 94-95 0.7970776589296449 1107 96-97 0.43697188742114434 1107 98-99 0.6434066952749902 1107 100-101 0.8273049925535751 1108 1 -0.1308988262026638 1108 2 -0.12509998875406225 1108 3 -0.10301767199352696 1108 4 -0.07891104761561962 1108 5 -0.031450432608622236 1108 6 -0.04025080333484965 1108 7 -0.07209230010599299 1108 8 -0.04523428519565442 1108 9 -0.12900381678574746 1108 10-11 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1.719348065779048 2316 72-73 0.7444303892939992 2316 74-75 0.211727808754528 2316 76-77 -0.1348066096237872 2316 78-79 -0.3015139032879297 2316 80-81 -0.35434607969786214 2316 82-83 -0.198424472999438 2316 84-85 -0.4012824498457306 2316 86-87 -0.1745937121393375 2316 88-89 -0.2868863860348583 2316 90-91 -0.35276709499841985 2316 92-93 -0.40479387488798935 2316 94-95 -0.08296296340256504 2316 96-97 -0.10685722700881328 2316 98-99 -0.3629425224933236 2316 100-101 -0.44914028959658125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 46.0 4 137.0 5 251.0 6 586.0 7 1569.0 8 3608.0 9 5867.0 10 9139.0 11 13555.0 12 19214.0 13 23611.0 14 28465.0 15 34544.0 16 41568.0 17 48102.0 18 45946.0 19 42979.0 20 43318.0 21 45326.0 22 49469.0 23 57226.0 24 68006.0 25 84407.0 26 103023.0 27 127680.0 28 155911.0 29 188012.0 30 222940.0 31 266141.0 32 316162.0 33 384615.0 34 477109.0 35 603607.0 36 752632.0 37 774071.0 38 379148.0 39 25121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31696090665842 18.443227175643386 13.43752417595927 24.802287741738922 2 16.60856396623103 20.355025845303786 37.61624138773175 25.420168800733435 3 19.79203467717682 23.38653628553776 29.33781164806428 27.483617389221145 4 13.809141413222822 15.708603835411278 35.14828538564011 35.33396936572579 5 14.449711645106417 36.815950560698745 33.60033098670082 15.134006807494012 6 34.34334879383471 34.80551037107775 16.271519375475602 14.579621459611936 7 29.927289849808535 31.684536016941756 19.928169859964477 18.460004273285225 8 27.317517768764567 34.26619921409031 19.217970432026195 19.198312585118938 9 26.8682349219262 15.699822105671398 19.11332454792923 38.31861842447317 10-11 26.0677655586806 25.301587196605475 26.737537797722226 21.893109446991694 12-13 26.542732209235183 22.43898213505896 27.288021812493902 23.73026384321195 14-15 23.3346910107973 23.988801273681503 24.941600578638827 27.734907136882363 16-17 23.377318644413435 27.286603877749926 25.641504251709275 23.694573226127368 18-19 23.495508733136276 25.91317588316552 27.454002016123475 23.13731336757473 20-21 23.9471431728757 25.12941568979109 27.48516980797223 23.43827132936098 22-23 23.826789383072462 25.01462856130832 27.481284168139624 23.677297887479586 24-25 23.64898641715767 25.320703192674344 27.461388391982023 23.568921998185964 26-27 23.33884734497951 25.548313158872798 27.4305942768307 23.682245219316997 28-29 23.44181867134389 25.887283507527354 27.246991682063026 23.423906139065735 30-31 23.437239492454715 25.580824752363107 27.013105857589803 23.968829897592375 32-33 23.190008972068455 25.599331325067126 27.130195672297475 24.080464030566947 34-35 23.93239738550948 25.61730924125686 26.97858823685038 23.471705136383278 36-37 23.92159477053266 25.3456113144517 27.112717402148633 23.62007651286701 38-39 23.540323934599627 25.47818868926548 27.09215194426408 23.889335431870812 40-41 23.72704152520538 25.398057852478367 27.233384134034406 23.641516488281848 42-43 23.479300344090056 25.36980025857329 27.056632331223916 24.09426706611274 44-45 23.372774237030683 25.409311436942595 27.34260072739711 23.875313598629614 46-47 23.674804904604194 25.73764254849504 26.84575902080031 23.741793526100462 48-49 23.460357710600668 25.83007781074419 26.676489165982463 24.03307531267268 50-51 23.359033689412854 26.013453037249196 26.803274912901692 23.824238360436254 52-53 23.77260627993101 25.583167770657862 26.703996365695126 23.940229583716004 54-55 23.19432493060947 25.781807372722437 26.834104094165607 24.189763602502488 56-57 23.108506656973017 25.88938210690632 26.747577169098474 24.254534067022192 58-59 23.16431087339168 25.513299506730853 26.737326531019107 24.585063088858362 60-61 22.90116189605635 25.835883043299518 26.985735251223563 24.277219809420565 62-63 22.96093358083167 25.75273982663419 26.90792089912924 24.378405693404893 64-65 22.664227246738093 25.85372960801231 26.680662464926296 24.801380680323305 66-67 22.406088486300206 25.821381315715243 26.963492303279086 24.809037894705465 68-69 22.495206081978235 25.505700816900788 27.27813837789993 24.72095472322105 70-71 22.43718784825704 25.57376529103176 26.875271366128562 25.113775494582637 72-73 22.552246205142914 25.841019582877 26.613505031940875 24.993229180039208 74-75 22.75391813063241 26.15777861169847 26.049462850119582 25.03884040754954 76-77 22.819274759200585 25.900233698768876 26.136229667825774 25.144261874204766 78-79 22.871595400083983 26.20369867671069 25.97772283708216 24.94698308612317 80-81 23.043969195144328 26.098485808844252 25.823106507799505 25.03443848821191 82-83 22.842545916246152 26.151771126727574 25.910135681448697 25.09554727557758 84-85 22.639886814436707 26.242422282755573 25.771458785320196 25.346232117487528 86-87 22.765891952534435 26.172439903535903 25.740109679084096 25.321558464845566 88-89 22.727651438080176 26.28302858247793 25.408528124789008 25.58079185465288 90-91 22.503427518715284 26.242718388632163 25.565234005946085 25.688620086706464 92-93 22.541391223525604 26.044303189902795 25.67347633549064 25.74082925108096 94-95 22.246820952246647 25.784184111596314 26.046114314349524 25.922880621807522 96-97 22.519553969304404 25.47632782919864 25.958946793670883 26.045171407826075 98-99 22.380241432757053 25.867133888695438 25.648063843060438 26.104560835487067 100-101 22.130644598373248 25.682291576233574 25.85345185477531 26.33361197061787 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 639.0 1 462.5 2 423.0 3 584.5 4 542.5 5 567.5 6 952.5 7 1796.5 8 2761.0 9 3128.0 10 2770.0 11 2482.5 12 2595.0 13 2839.0 14 3152.0 15 3465.0 16 3832.5 17 4301.5 18 4842.0 19 5392.0 20 6101.5 21 7220.0 22 8729.0 23 10573.0 24 12745.0 25 15679.5 26 19716.0 27 24477.0 28 29336.5 29 34739.5 30 40753.0 31 50769.0 32 64263.5 33 69234.0 34 68427.0 35 71856.5 36 79631.5 37 88993.5 38 99829.5 39 108906.0 40 117693.5 41 129484.0 42 140788.5 43 154276.0 44 168350.0 45 183109.0 46 197712.5 47 213784.5 48 227734.0 49 230710.0 50 229325.0 51 230505.5 52 233053.0 53 238035.5 54 238838.0 55 227344.0 56 211711.5 57 195344.0 58 181110.0 59 162564.5 60 134142.0 61 107367.0 62 86287.5 63 65672.0 64 47659.0 65 35169.5 66 24994.5 67 17892.0 68 13343.5 69 10217.5 70 7780.0 71 5601.0 72 4219.0 73 3589.0 74 2808.0 75 2009.0 76 1417.5 77 906.0 78 559.0 79 332.0 80 191.0 81 103.0 82 75.5 83 49.0 84 28.0 85 15.5 86 9.5 87 9.5 88 4.5 89 1.5 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022891287146211574 2 0.024214058153055263 3 0.0 4 0.0 5 0.0 6 1.8371819539495645E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.5720547355293904E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.674363907899129E-5 26-27 2.939491126319303E-4 28-29 4.5929548848739115E-5 30-31 3.3987866148066945E-4 32-33 0.0021219451568117473 34-35 0.004096915757307529 36-37 0.10052141061035041 38-39 0.09270420139629502 40-41 0.10159616205341092 42-43 0.10268009940624116 44-45 0.09620403301856895 46-47 0.08969122299181774 48-49 0.09046283941247656 50-51 0.08942483160849506 52-53 0.07053860112189353 54-55 0.059818644420597825 56-57 0.03227928693089385 58-59 0.013879909662088961 60-61 0.009305326596754544 62-63 0.0021954324349697297 64-65 0.005419686764151215 66-67 0.15436002777084243 68-69 0.412419790932368 70-71 0.39457156824974804 72-73 0.18146764750136823 74-75 0.02978071947352244 76-77 0.0314617409613863 78-79 0.005153295380828528 80-81 0.0014881173826991473 82-83 3.674363907899129E-5 84-85 4.041800298689042E-4 86-87 0.0010563796235209997 88-89 2.1127592470419993E-4 90-91 7.624305108890693E-4 92-93 0.0027098433820756077 94-95 0.07836499624571867 96-97 0.017223580818277167 98-99 0.01881274320844354 100-101 0.010279033032347814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5443119.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.910587328546164 #Duplication Level Percentage of deduplicated Percentage of total 1 63.04905175771249 20.119322720965116 2 14.282262865103867 9.115107928122978 3 6.686160103123797 6.400778875901195 4 4.012783548969691 5.122011194798029 5 2.6014259575093734 4.1506515097924845 6 1.77931908783639 3.4067470282651335 7 1.29020002306345 2.8819728355080945 8 0.9503490416253723 2.4260956868309274 9 0.7568024999355044 2.173501103818857 >10 4.145172820667748 23.73786865405516 >50 0.28135140622025984 6.175279593567686 >100 0.15167109221960595 8.96921351458529 >500 0.008312323557282205 1.797297046043159 >1k 0.004848850407098144 2.833173835197511 >5k 2.3089763843250343E-4 0.47207283787781146 >10k+ 5.772440960812586E-5 0.2189056346706692 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11884 0.21833070340736624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8862 0.16281106475901042 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6008 0.11037789179328984 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5601 0.10290056124071512 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.8371819539495645E-5 3 0.0 0.0 0.0 0.0 1.8371819539495645E-5 4 0.0 1.8371819539495645E-5 0.0 0.0 3.674363907899129E-5 5 0.0 7.348727815798258E-5 0.0 0.0 3.674363907899129E-5 6 0.0 7.348727815798258E-5 0.0 0.0 3.674363907899129E-5 7 0.0 7.348727815798258E-5 0.0 0.0 3.674363907899129E-5 8 0.0 7.348727815798258E-5 0.0 0.0 3.674363907899129E-5 9 0.0 7.348727815798258E-5 0.0 0.0 5.511545861848694E-5 10-11 0.0 7.348727815798258E-5 0.0 0.0 5.511545861848694E-5 12-13 0.0 1.1023091723697387E-4 0.0 0.0 5.511545861848694E-5 14-15 0.0 2.1127592470419993E-4 0.0 9.185909769747822E-6 7.348727815798258E-5 16-17 0.0 2.755772930924347E-4 0.0 4.5929548848739115E-5 9.185909769747823E-5 18-19 0.0 2.939491126319303E-4 0.0 8.267318792773041E-5 9.185909769747823E-5 20-21 0.0 3.12320932171426E-4 0.0 1.6534637585546082E-4 1.0104500746722605E-4 22-23 0.0 3.12320932171426E-4 0.0 3.12320932171426E-4 1.194168270067217E-4 24-25 0.0 3.3069275171092164E-4 0.0 8.359177890470519E-4 1.2860273677646952E-4 26-27 0.0 3.4906457125041725E-4 0.0 0.002121945156811747 1.6534637585546082E-4 28-29 0.0 3.674363907899129E-4 0.0 0.009672762987544457 1.6534637585546082E-4 30-31 0.0 3.949941200991564E-4 0.0 0.03996789340817278 1.7453228562520863E-4 32-33 0.0 4.2255184940839986E-4 0.0 0.10713526564456885 2.2046183447394776E-4 34-35 0.0 4.776673080268868E-4 0.0 0.19958226156731096 2.388336540134434E-4 36-37 0.0 6.24641864342852E-4 0.0 0.3500015340469316 2.388336540134434E-4 38-39 0.0 9.277768867445302E-4 0.0 0.5924085069608068 2.388336540134434E-4 40-41 0.0 0.0011298669016789821 0.0 0.8682154477974853 2.5720547355293904E-4 42-43 0.0 0.0013227710068436866 0.0 1.145143069626073 2.5720547355293904E-4 44-45 0.0 0.0013411428263831822 0.0 1.4649413323500735 2.5720547355293904E-4 46-47 0.0 0.0013595146459226778 0.0 1.8351886115295293 2.755772930924347E-4 48-49 0.0 0.0014421878338504081 0.0 2.2220623873922287 2.939491126319303E-4 50-51 0.0 0.0014789314729293994 0.0 2.653708287472679 2.939491126319303E-4 52-53 0.0 0.0014881173826991473 0.0 3.0612871039563894 3.3069275171092164E-4 54-55 0.0 0.001506489202238643 0.0 3.491141751631739 3.766223005596608E-4 56-57 0.0 0.0015248610217781385 0.0 3.965309228036352 4.041800298689042E-4 58-59 0.0 0.0015524187510873822 0.0 4.4478818118802845 4.041800298689042E-4 60-61 0.0 0.0016167201194756168 0.0 4.939649491403734 4.2255184940839986E-4 62-63 0.0 0.0016167201194756168 0.0 5.414561026499696 4.2255184940839986E-4 64-65 0.0 0.0016167201194756168 0.0 5.9029115475888 4.2255184940839986E-4 66-67 0.0 0.0016902073976335994 0.0 6.4278128036517295 4.2255184940839986E-4 68-69 0.0 0.0016902073976335994 0.0 6.998450704458234 4.2255184940839986E-4 70-71 0.0 0.0016993933074033471 0.0 7.629743167474384 4.317377591781477E-4 72-73 0.0 0.001708579217173095 0.0 8.287702326552111 4.960391275663825E-4 74-75 0.0 0.001717765126942843 0.0 8.945845203825233 4.960391275663825E-4 76-77 0.0 0.001818810134410069 0.0 9.645113399137518 5.052250373361303E-4 78-79 0.0 0.001818810134410069 1.8371819539495645E-5 10.41031805477705 5.144109471058781E-4 80-81 0.0 0.001818810134410069 1.8371819539495645E-5 11.216188732967257 5.327827666453738E-4 82-83 0.0 0.0018279960441798166 1.8371819539495645E-5 12.024943786825164 5.69526405724365E-4 84-85 0.0 0.0018371819539495645 1.8371819539495645E-5 12.805994136817512 5.69526405724365E-4 86-87 0.0 0.0018371819539495645 1.8371819539495645E-5 13.598306412187572 5.69526405724365E-4 88-89 0.0 0.0018371819539495645 1.8371819539495645E-5 14.42590176698323 5.878982252638606E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16420 0.0 35.029728 1 GGTATCA 10640 0.0 33.496975 1 ATCAACG 20135 0.0 28.294315 3 TCAACGC 20275 0.0 28.075542 4 TATCAAC 20585 0.0 27.820435 2 GTACATG 18965 0.0 27.776495 1 CAACGCA 20515 0.0 27.770216 5 TACATGG 18505 0.0 27.742508 2 AACGCAG 21160 0.0 26.94614 6 ACATGGG 20055 0.0 24.88289 3 ACGCAGA 24305 0.0 23.342283 7 CGCAGAG 25270 0.0 22.432127 8 GAGTACA 14020 0.0 21.19013 1 CATGGGG 13280 0.0 20.788958 4 GAGTACT 12420 0.0 20.414272 12-13 AGAGTAC 20440 0.0 20.190456 10-11 GCAGAGT 27315 0.0 20.058043 9 CATGGGA 7380 0.0 19.54002 4 GTATAGA 1220 0.0 19.060907 1 AGTACTT 13060 0.0 18.996181 12-13 >>END_MODULE